| cell-surface carbohydrates of entamoeba invadens. | cell-surface carbohydrates of entamoeba invadens trophozoites were analyzed using (a) a panel of highly purified lectins specific for molecules containing n-acetylglucosamine or sialic acid, n-acetylgalactosamine, galactose, mannose-like residues, and fucose; (b) escherichia coli k-12 with mannose-sensitive fimbria; (c) enzymatic digestion; and (d) scanning electron microscopy. the presence of galactose (d-gal) and n-acetylgalactosamine (d-galnac) was detected in the amoeba. previous trypsinizat ... | 1997 | 9342747 |
| a study of glycoconjugates in nasopharyngeal carcinoma with correlation to clinical transformation. | little is known about the glycoconjugate changes in human nasopharyngeal epithelium following neoplastic changes. glycoconjugate histochemistry (glycine maximus (sba), griffonia simplicifolia ii (gsa-ii), ulex europaeus (uea-i), arachis hypogaea (pna) and canavalia ensiformis (cona)) were performed on the following nasopharyngeal biopsies: 10 adenoid tissues (benign controls), 10 chronic inflammation, 20 squamous metaplasia, 20 undifferentiated carcinoma and 5 squamous cell carcinoma. these resu ... | 1997 | 9408149 |
| lectin binding sites on cd34+ human haematopoietic stem cells and lymphocytes from peripheral blood: an ultrastructural post-embedding study. | this study was performed to obtain a better insight into the glycosylation pattern of human cd34+ haematopoietic stem cells and lymphocytes from peripheral blood using an ultrastructural post-embedding technique. lectins applied were derived from canavalia ensiformis (con a), triticum vulgare (wga), lycopersicon esculentum (lea), limulus polyphemus (lpa), ulex europaeus-i (uea-i), bauhinia purpurea (bpa), glycine max (sba), helix pomatia (hpa), arachis hypogaea (pna) and erythrina cristagalli (e ... | 1997 | 9413743 |
| anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen paracoccidioides brasiliensis. | the surface anionogenic groups and sialoglycoconjugate structures of paracoccidioides brasiliensis yeast forms were analysed by cell microelectrophoresis, binding assays with lectins and viral particles, ultrastructural cytochemistry, enzymic digestion and flow cytofluorimetry. p. brasiliensis yeast forms, particularly the budding primordia, reacted strongly with cationized ferritin. binding assays showed that the reaction with sialic-acid-specific limax flavus lectin (lfa) was distributed over ... | 1998 | 9493368 |
| lectins for drug delivery within the oral cavity--investigation of lectin binding to oral mucosa. | the aim of this study was to identify receptors present on the buccal mucosa in order to select appropriate lectins that will allow the retention of a dosage form within the oral cavity. studies using human buccal cells, the avidin-biotin-complex/diaminobenzidine method for identifying lectin binding and a microdensitometer to allow a semi-quantitative analysis of stain intensity, showed a wide diversity of lectin receptors. kinetic studies of lectin binding to buccal cells revealed significant ... | 1997 | 9524313 |
| evaluation of stimulus-induced acrosome reaction by two-colour flow cytometric analysis. | acrosome status in human spermatozoa from 20 normozoospermic men was evaluated by flow cytometry following the induction of the acrosome reaction with the ionophore a23187. dual fluorescence staining of methanol fixed spermatozoa incubated with and without (control) the ionophore a23187 was performed with probes which targeted the outer acrosomal membrane (oam) (rhodamine-labelled arachis hypogaea agglutinin) or constituents of the acrosomal vesicle (fluorescein-labelled pisum sativum agglutinin ... | 1998 | 9570270 |
| identification and molecular characterization of beta-hemolytic streptococci isolated from harbor porpoises (phocoena phocoena) of the north and baltic seas. | the present study was designed to identify and comparatively investigate 35 beta-hemolytic streptococci isolated from stranded harbor porpoises or from animals caught in fishing nets of the north and baltic seas. according to biochemical and serological data and to lectin agglutination tests with the lectin of arachis hypogaea, all 35 isolates could be classified in lancefield's serological group l and could be identified as streptococcus dysgalactiae subsp. dysgalactiae. all 35 group l streptoc ... | 1998 | 9650933 |
| mesenchymal changes associated with retinoic acid induced cleft palate in cd-1 mice. | retinoic acid (ra) is teratogenic in many species and is an effective inducer of cleft palate in mice. the pathogenesis of cleft formation varies with the timing of exposure. it has been demonstrated, before formation of the palatal shelves, that ra exposure results in insufficient mesenchymal tissue, and palatal shelves fail to make contact. however, at the palatal shelf outgrowth stage, ra exposure affects shelf elevation and growth in rats, and possibly medial edge epithelium (mee) differenti ... | 1998 | 9672841 |
| a phytoalexin is modified to less fungitoxic substances by crown rot pathogen in groundnut arachis hypogaea l. | phytoalexins from four different treatments viz. control, af 1-treated, a. niger-treated, and dual inoculated were separated by tlc showed that one phytoalexin with an rf value of 0.628 (p1) appeared on 2nd day only in dual-inoculated seeds of groundnut (a. hypogaea). by 3rd day three additional phytoalexins were visualized in response to a. niger-treatment with lower rf values 0.485 (p2), 0.388 (p3) and 0.314 (p4) as compared to p1. in dual inoculated seedlings, p1 and p3 could be visualized wh ... | 1998 | 9731478 |
| a flow cytometric technique using peanut agglutinin for evaluating acrosomal loss from human spermatozoa. | flow cytometric methods for the quantification of acrosome-reacted ejaculated human spermatozoa are described in which fluorescence-labeled peanut agglutinin (arachis hypogaea) binding to the outer acrosomal membrane is used after incubation with ethidium homodimer (ehd) as vital dye and membrane permeation using methanol. fluorescein-labeled fucoidan (f-fucoidin) was shown to bind to sperm that were also stained by propidium iodide (pi) and ehd; therefore, the use of f-fucoidin as vital dye was ... | 1998 | 9796613 |
| characterisation of cytotrophoblastic-like cells present in subinvolutioned placental sites of the bitch. | this paper describes an approach to study the cells present in the subinvolution of placental sites (sips), a pathological post partum condition of the bitch that causes persistent hemorrhage of the genital tract. the expression of intermediate filament proteins was examined to determine the fetal or maternal origin of the cytotrophoblastic-like cells found in this entity. lectin binding on tissue sections were also studied to characterise cellular glycoconjugates. image processing and morphomet ... | 1998 | 9810494 |
| lectin histochemistry of dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats. | a lectin histochemical study was carried out on the dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats (htrs) and wistar rats (wrs) at 3, 7 and 24 weeks of age to clarify the lectinhistochemical characteristics of the skin during their development. the lectins examined were concanavalia ensiformis (con a), dolichos biflorus agglutinin (dba), griffonia simpliciolia (gs-i), helix pomatia agglutinin (hpa), arachis hypogaea (pna), glycine maximus agglutinin (sba), ulex europeus agglutinin ( ... | 1998 | 9816494 |
| localization of lectin-binding sites on the surface of trypanosoma cruzi grown in chemically defined conditions. | the transformation of trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (tau 3aag medium). during this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using fitc and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. the pattern of labeling with the lectins from triticum vulgaris (wga), arachis h ... | 1998 | 9826132 |
| s-fimbriae from escherichia coli bind to soluble glycoproteins from human milk. | escherichia coli (e. coli) strains, expressing s-fimbriae, belong to the most common gram-negative pathogens that cause sepsis and meningitis in neonates. the attachment of s-fimbriae to the cell surface is mediated by membrane glycoconjugates, which often carry n-acetylneuraminic acid. | 1999 | 10067725 |
| lectin binding studies of parotid salivary glycoproteins in sjögren's syndrome. | human parotid salivas were collected from patients with secondary sjögren's syndrome and controls without disease or with drug-induced xerostomia. parotid glycoproteins were separated by gradient sodium dodecyl sulphate gel electrophoresis (sds-page), electroblotted onto nitrocellulose membrane and probed with biotinylated lectins of characterised sugar specificities. the binding patterns of lectins from maclura pomifera (mpa) and arachis hypogaea (pna) indicated that many parotid glycoproteins ... | 1999 | 10451125 |
| distribution of glycosaminoglycans in rat renal tubular epithelium. | polyanionic constituents of the glomerular capillary wall have been previously shown to have a primary role in the control of glomerular filtration. in the study presented here, the distribution and biochemical nature of polyanionic constituents in proximal (pt) and distal (dt) tubules have been investigated as possible determinants of tubulointerstitial function. for histochemical localization of sialic acid, paraffin sections were treated with arachis hypogaea lectin (pna) before and after neu ... | 1997 | 10495788 |
| expression, purification and characterization of peanut (arachis hypogaea) agglutinin (pna) from baculovirus infected insect cells. | peanut (arachis hypogaea) seed lectin, pna is widely used to identify tumor specific antigen (t-antigen), galbeta1-3galnac on the eukaryotic cell surface. the functional amino acid coding region of a cdna clone, pbsh-pn was pcr amplified and cloned downstream of the polyhedrin promoter in the autographa californica nucleopolyhedrovirus (acnpv) based transfer vector pvl1393. co-transfection of spodoptera frugiperda cells (sf9) with the transfer vector, pacpna and acrp6 (a recombinant acnpv having ... | 1999 | 10513900 |
| o-linked glycosylation occurs on basic parotid salivary proline-rich proteins. | interactions between salivary glycoproteins and many oral bacteria have been shown to depend on o-linked glycans on salivary glycoproteins. basic proline-rich proteins form the largest group of proteins within human parotid saliva. in the present study human parotid salivary glycoproteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis or two-dimensional electrophoresis, electroblotted onto nitrocellulose and probed with two biotin-labelled lectins from maclura pomife ... | 1999 | 10551158 |
| phylogeny and diversity of bradyrhizobium strains isolated from the root nodules of peanut (arachis hypogaea) in sichuan, china. | twenty-two rhizobial strains isolated from the root nodules of two chinese peanut cultivars (arachis hypogaea l. tianfu no. 3 and a local cultivar) growing at four different sites in the sichuan province, southwest china, were characterized by growth rate, rep-pcr, pcr-rflp of 16s rdna, partial sequencing of ribosomal genes, and fatty acid-methyl ester analysis (fame), and compared with strains representing bradyrhizobium japanicum, b. elkanii and other unclassified bradyrhizobium sp. all peanut ... | 1999 | 10553290 |
| lectin histochemistry of dog major and minor salivary glands. | the distribution of different carbohydrates in dog major and minor salivary glands was investigated using a peroxidase-labelled avidin-biotin method to demonstrate binding of six lectins (canavalia ensiformis agglutinin [con a], dolichos biflorus agglutinin [dba], arachis hypogaea (peanut) agglutinin [pna], glycine max agglutinin [sba], tetragonolobus purpurea agglutinin [tgp] and wheat germ agglutinin [wga]). with pna, there was only weak staining in serous acini of parotid glands. other lectin ... | 1999 | 10596406 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| constitutive accumulation of a resveratrol-glucoside in transgenic alfalfa increases resistance to phoma medicaginis. | alfalfa (medicago sativa) was transformed with a peanut (arachis hypogaea) cdna encoding resveratrol synthase (rs) transcriptionally regulated by an enhanced cauliflower mosaic virus (camv) 35s promoter. transgenic plants accumulated a new compound, not present in wild-type or vector-transformed alfalfa, that was identified as trans-resveratrol-3-o-beta-d-glucopyranoside (rgluc) by high-pressure liquid chromatography (hplc), uv, 1h- and 13c-nuclear magnetic resonance (nmr) analyses. rgluc concen ... | 2000 | 10796021 |
| a peanut seed lipoxygenase responsive to aspergillus colonization. | several lines of evidence have indicated that lipoxygenase enzymes (lox) and their products, especially 9s- and 13s-hydroperoxy fatty acids, could play a role in the aspergillus/seed interaction. both hydroperoxides exhibit sporogenic effects on aspergillus spp. (calvo, a., hinze, l., gardner, h.w. and keller, n.p. 1999. appl. environ. microbiol. 65: 3668-3673) and differentially modulate aflatoxin pathway gene transcription (burow, g.b., nesbitt, t.c., dunlap, j. and keller, n.p. 1997. mol. pla ... | 2000 | 10809442 |
| double electrophoretic separation and lectin analyses of the component chains of secretory immunoglobulin a from human saliva. | a new method is presented for the separation of secretory immunoglobulin a (siga) from salivary samples. salivary proteins (from parotid or stimulated whole mouth saliva) were precipitated with methanol to concentrate siga from salivary samples whilst removing other salivary proteins. siga purified from breast milk and salivary proteins was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) under nonreducing conditions. following completion of electrophoresis t ... | 2000 | 10832871 |
| coculture of chicken thrombocytes and monocytes: morphological changes and lectin binding. | chicken leukocytes were separated from blood on a percoll cushion following adherence on coverslips, resulting in a coculture of thrombocytes and monocytes. this system was characterized by light microscopy, scanning and transmission electron microscopy, by lectin binding and actin localization in thrombocytes. during the first 24 hours nuclear condensation, cytoplasmic shrinkage, detachment and death of thrombocytes were observed. in contrast, monocytes gradually increased their spreading capac ... | 1998 | 10904525 |
| reaction of various lectins to mucin derived from the different layers of rat gastric mucosa: comparison of enzyme-linked lectin binding assay with lectin histochemistry. | the purpose of this study was to demonstrate that the histochemical staining reactivities of lectins in rat stomach actually represent the gastric mucins, and to estimate the utility of the lectins for mucin histochemistry. in this paper, the lectin histochemistry was compared with an enzyme-linked lectin binding assay (ella) of the mucins derived from distinct regions and layers of the sprague-dawley rat stomach and it was examined to determine the definite binding and problematic binding of th ... | 2000 | 11041246 |
| identification of perivitelline n-linked glycans as mediators of sperm-egg interaction in chickens. | this study demonstrates that carbohydrates play an essential role in sperm-egg interactions in birds. sperm-egg interaction was measured in vitro as the ability of spermatozoa to hydrolyse a small hole in the inner perivitelline layer, the equivalent of the mammalian zona pellucida. preincubation with triticum vulgaris lectin (wga) and succinyl-wga (s-wga) at 10 microgram ml(-1) resulted in complete inhibition of sperm-egg interaction, whereas at the same concentration a range of other lectins ( ... | 2000 | 11058456 |
| chemoenzymatic synthesis of glycopolypeptides carrying alpha-neu5ac-(2-->3)-beta-d-gal-(1-->3)-alpha-d-galnac, beta-d-gal-(1-->3)-alpha-d-galnac, and related compounds and analysis of their specific interactions with lectins. | glycopolypeptide (1) carrying the beta-d-gal-(1-->3)-alpha-d-galnac unit as a kind model of asialo-type mucin was synthesized through three steps: enzymatic synthesis of p-nitrophenyl disaccharide glycoside, reduction of the p-nitrophenyl group, and coupling of the amino group with the carboxyl group of poly(l-glutamic acid)s (pga). in a similar manner, glycopolypeptides (2-7) carrying beta-d-gal-(1-->3)-beta-d-galnac, beta-d-gal-(1-->3)-beta-d-glcnac, beta-d-gal-(1-->6)-alpha-d-galnac, beta-d-g ... | 2000 | 11097173 |
| opposite effects on human colon cancer cell proliferation of two dietary thomsen-friedenreich antigen-binding lectins. | increased cell surface expression of the thomsen-friedenreich antigen (tf antigen, galbeta1-3galnacalpha-) is a common feature in malignant and pre-malignant epithelia. our previous studies have shown that dietary tf-binding lectins from peanut (arachis hypogea) and edible mushroom (agaricus bisporus) produce marked but different effects on human intestinal epithelial cell proliferation. this study investigates the proliferative effects of the other two known dietary tf-binding lectins: jacalin ... | 2001 | 11169464 |
| analysis of glycoconjugate patterns of normal and hormone-induced dysplastic noble rat prostates, and an androgen-independent noble rat prostate tumor, by lectin histochemistry and protein blotting. | alteration of the expression of glycoconjugates is frequently observed in tumors. however, studies on the changes of cellular glycosylation in the early premalignant stage of prostate carcinogenesis are scarce. | 2001 | 11170128 |
| molecular properties of the oleoyl-phosphatidylcholine desaturase from arachis hypogaea l. | deficiencies in the activity of the microsomal oleoyl phosphatidylcholine (oleoyl-pc) desaturase from peanuts are the basis of the high oleate oil. mutation of aspartate-150 to asparagine and the attendant decrease in activity, together with the loss in expression of the higher activity transcript, was the molecular basis of the high oleate trait. the ability of oleoyl-pc desaturase to desaturate palmitoleate, oleate and 10(z) nonadecenoate to methylene-interrupted products was not consistent wi ... | 2000 | 11171148 |
| genetic diversity of bradyrhizobium strains isolated from arachis hypogaea. | rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. knowledge about the indigenous population is necessary for the selection and application of inoculant strains. in this study, we have assessed the genetic diversity of bradyrhizobium strains isolated from the host plant, arachis hypogaea along the coastline of tamil nadu. different populations collected from varying environmental conditions were analysed for salt and ph toleran ... | 2001 | 11261490 |
| peanut (arachis hypogaea) lectin recognizes alpha-linked galactose, but not n-acetyl lactosamine in n-linked oligosaccharide terminals. | peanut (arachis hypogaea) agglutinin (pna) is extensively used as tumour marker as it strongly recognises the cancer specific t antigen (galbeta1-->3galnac-), but not its sialylated version. however, an additional specificity towards galbeta1-->4glcnac (lacnac), which is not tumour specific, had been attributed to pna. for correct interpretation of lectin histochemical results we examined pna sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactos ... | 2001 | 11325423 |
| hypogin, a novel antifungal peptide from peanuts with sequence similarity to peanut allergen. | a protein designated hypogin, with a prominent suppressive action on the growth of the fungi mycosphaerella arachidicola, fusarium oxysporum and coprinus comatus, was isolated from seeds of the peanut arachis hypogaea. the protein inhibited human immunodeficiency virus (hiv) reverse transcriptase and enzymes associated with hiv infection including alpha-glucosidase and beta-glucosidase. the proliferative response of mouse splenocytes was attenuated in the presence of the protein. the protein exh ... | 2001 | 11328490 |
| endoplasmic oleoyl-pc desaturase references the second double bond. | the regiospecificity for the gene product of fad2,(1) the microsomal oleoyl-pc desaturase from higher plants, differs from some previous suggestions. rather than only referencing the carboxyl group (a delta(12) desaturase) or the methyl terminus (an omega-6 desaturase), this desaturase locates the second double bond in its substrates by first referencing the existing double bond. this specificity was demonstrated for the oleoyl-pc desaturase cdna from the developing seeds of peanut (arachis hypo ... | 2001 | 11397429 |
| patient-tailored cloning of allergens by phage display: peanut (arachis hypogaea) profilin, a food allergen derived from a rare mrna. | a peanut cdna phage surface display library was constructed and screened for the presence of ige-binding proteins. we used a serum from a peanut-sensitized individual with a low specific ige level to peanut extract and suffering from mild symptoms after peanut ingestion. a total of 10(11) cdna clones were screened by affinity selection towards serum ige immobilized to solid-phase supports. after five rounds of selective enrichment, sequence determination of 25 inserts derived from different clon ... | 2001 | 11419720 |
| adenovirus-mediated expression of pig alpha(1, 3) galactosyltransferase reconstructs gal alpha(1, 3) gal epitope on the surface of human tumor cells. | gal alpha(1, 3) gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by alpha(1, 3) galactosyltransferase [alpha(1, 3) gt] enzyme on the cells of lower mammalian animals such as pigs and mice. human has no gal epitope due to the inactivation of alpha(1, 3) gt gene but produces a large amount of antibodies (anti-gal) which recognize gal alpha(1, 3) gal structures specifically. in this study, a replication-deficient recombinant adenoviral vector ad5sgt containing pig alpha(1 ... | 2001 | 11453543 |
| final report on the safety assessment of peanut (arachis hypogaea) oil, hydrogenated peanut oil, peanut acid, peanut glycerides, and peanut (arachis hypogaea) flour. | peanut (arachis hypogaea) oil is the refined fixed oil obtained from the seed kernels of arachis hypogaea. hydrogenated peanut oil, peanut acid, and peanut glycerides are all derived from peanut oil. peanut flour is a powder obtained by the grinding of peanuts. the oils and glycerides function in cosmetic formulations as skin-conditioning agents. the acid functions as a surfactant-cleansing agent, and the flour functions as an abrasive, bulking agent and/or viscosity-increasing agent. in 1998, o ... | 2001 | 11558642 |
| investigation of lotic microbial aggregates by a combined technique of fluorescent in situ hybridization and lectin-binding-analysis. | a technique combining fluorescent in situ hybridization and lectin-binding-analysis (fish-lba) was developed and applied for the simultaneous detection of cellular components and glycoconjugates in lotic microbial aggregates (river snow). river snow aggregates were directly collected from the bulk water phase into coverslip chambers, in which the complete procedure including fixation, fluorescent in situ hybridization, lectin-binding and optical analysis by confocal laser scanning microscopy was ... | 2002 | 11777585 |
| characterization of trophoblast cell populations by lectin histochemistry in canine placenta during development. | the aim of this study was to identify and characterize populations of trophoblast cells in canine placenta during different stages of fetal development using lectin histochemistry. dogs have endotheliochorial placentation and trophoblast cell invasion continues after chorioallantois villous penetration early in pregnancy, leading to formation of a labyrinth. specialized subpopulations of cells differentiate, such as syncytial trophoblast that invades the maternal epithelium early in placentation ... | 2001 | 11787150 |
| peanut and amaranthus leucocarpus lectins discriminate between memory and naive/quiescent porcine lymphocytes. | lectins are relevant tools to isolate and characterize different cellular sub-populations. in this work, we used the lectins arachis hypogaea (peanut agglutinin, pna) and amaranthus leucocarpus (all), specific for galss1, 3galnac, to characterize naive and memory lymphocytes from pigs, experimentally infected with the porcine rubulavirus (rvp). our results showed that both lectins recognized preferentially lymphocytes with the cd4(+)cd8(+) phenotype (p<0.05). the phenotypic analysis of the cells ... | 2002 | 11825599 |
| in vivo volatile emissions from peanut plants induced by simultaneous fungal infection and insect damage. | peanut plants, arachis hypogaea, infected with white mold. sclerotium rolfsii, emit a blend of organic compounds that differs both quantitatively and qualitatively from the blend emitted from plants damaged by beet armyworm (baw; spodoptera exigua) larvae or from uninfected, undamaged plants. attackby baw induced release of lipoxygenase products (hexenols, hexenals, and hexenyl esters), terpenoids, and indole. the plant-derived compound methyl salicylate and the fungal-derived compound 3-octanon ... | 2002 | 11868672 |
| trypanosoma cruzi i and trypanosoma cruzi ii: recognition of sugar structures by arachis hypogaea (peanut agglutinin) lectin. | epimastigote culture forms of different isolates of trypanosoma cruzi from different mammal hosts, humans, and vectors were tested with fitc-conjugated peanut agglutinin lectin (pna-fitc). the parasites maintained in axenic medium, liver infusion tryptose. were evaluated by flow cytometric analyses; whereas t. cruzi i (tcl), which is associated with the sylvatic transmission cycle, was labeled in high percentages with pna (88-99.2%), t. cruzi ii (tcii) (parasites associated with domiciliar cycle ... | 2002 | 12099431 |
| selection of alternative genetic sources of large-seed size in virginia-type peanut: evaluation of sensory, composition, and agronomic characteristics. | jenkins jumbo, the ancestral source of large-seed size in the virginia market type (arachis hypogaea l.), has been shown to have a deleterious effect on flavor of peanut. the pervasiveness of jenkins jumbo in the ancestry of large-seeded germplasm contributes to the generally less intense roasted peanut flavor of u.s. cultivars of the virginia market type. as a remedy to this problem, alternative sources of large-seed size were sought. nine large-seeded selections, with nc 7 and florunner as che ... | 2002 | 12166976 |
| allelopathic potential and chemical constituents of volatiles from ageratum conyzoides under stress. | the allelopathic potential of ageratum conyzoides was investigated under different environmental stress conditions, including nutrient deficiency. physical damage, 2.4-d treatment, competition with bidens pilosa, infection with erysiphe cichoracearum, and feeding by aphiids gossypii. the inhibitory effects of a. conyzoides volatiles on peanut (arachis hypogaea), redroot amaranth (amaranthus retroflexus), cucumber (cucumis sativus), and ryegrass (lolium multiforum) increased when plants were grow ... | 2002 | 12184395 |
| hypoglycemic and hypolipidemic effects of aqueous extract of arachis hypogaea in normal and alloxan-induced diabetic rats. | the hypoglycemic and hypolipidemic effect of aqueous extract of arachis hypogaea was investigated in normal and alloxan-induced diabetic rats. the extract caused a significant (p < 0.05) decrease of fasting blood glucose of both normal and alloxan-induced diabetic rats from 102.60 +/- 1.65 mg/dl to 88.79 +/- 0.94 mg/dl for normal and 189.0 +/- 30.79 mg/dl to 107.55 +/- 1.54 mg/dl for alloxan-induced diabetic rats. the extract also caused a significant (p < 0.05) decrease in serum triglyceride, t ... | 2002 | 12403165 |
| the atopic dog as a model of peanut and tree nut food allergy. | animal models are needed that mimic human ige-mediated peanut and tree nut allergy. atopic dogs have been previously used in a model of food allergy to cow's milk, beef, wheat, and soy, with the demonstration of specific ige production and positive oral challenges similar to those seen in human subjects. | 2002 | 12464960 |
| lectin histochemical study of lipopigments present in the cerebellum of solanum fastigiatum var. fastigiatum intoxicated cattle. | this study was carried out to investigate the pattern of lectin binding in the cerebellum of calves poisoned with solanum fastigiatum var. fastigiatum. for the experimental reproduction of the illness, s. fastigiatum var. fastigiatum was collected from farms where the intoxication occurs. the dried ground plant was administered to two 1-year-old cattle by a ruminal cannula. the animals received 5 g/kg b.w. daily, 5 days a week, during periods of 107 and 140 days. after these periods the animals ... | 2002 | 12489871 |
| [genetic diversity among chinese peanut rhizobia by rep-pcr analysis]. | repetitive sequences(repetitive extragenic palindromic[rep], and enterobacterial repetitive intergenic consensus [eric]) with the polymerase chain reaction(pcr) were used to fingerprint pure dna extracted from 79 bradyrhizobial strains. these strains included 59 peanut rhizobia [bradyrhizobium sp. (arachis)] isolated from root nodules of 24 peanut (arachis hypogaea l) cultivars from 23 sites in 11 provinces in china, 6 peanut rhizobial strains from other nations, and 14 reference strains of othe ... | 1999 | 12555567 |
| the role of surface carbohydrates on the interaction of microconidia of trichophyton mentagrophytes with epithelial cells. | the presence of carbohydrate-binding adhesins on the microconidia of trichophyton mentagrophytes surface and their role on cellular interactions were investigated. flow cytometry showed that this fungus recognizes the sugars mannose and galactose. the binding was inhibited by the addition of methyl alpha-d-mannopyranoside and methyl alpha-d-galactopyranoside, and showed higher fluorescence intensity at 37 degrees c than 28 degrees c. trypsin treatment and heating of the cells reduced the binding ... | 2003 | 12628546 |
| [genetic diversity and phylogeny of rhizobia isolated from peanut (arachis hypogaea)]. | forty three rhizobium strains isolated from peanut (arachis hypogaea) and 15 reference strains from other genus and species were analyzed by the method of 16s rrna rflp, 16s rrna sequencing and 16s-23s igs pcr rflp. the results of the 16s rrna rflp shown that 43 strains tested were all ascribed to the genus of bradyrhizobium phylogenetically. strains tested were adjacent to the b. japonicum and far from b. elkanii 16s rrna genotype. the genotypes generated by the 4 restriction endonucleases, mbo ... | 2002 | 12693105 |
| "chitin-specific" peroxidases in plants. | the activity of various plant peroxidases and the ability of their individual isoforms to bind chitin was studied. some increase in peroxidase activity was observed in crude extracts in the presence of chitin. activated peroxidases of some species fell in the fraction not sorbed on chitin and those of other species can bind chitin. only anionic isoperoxidases from oat (avena sativa), rice (oryza sativa), horseradish (armoracia rusticana), garden radish (raphanus sativus var. radicula), peanut (a ... | 2003 | 12693984 |
| the use of legume trap crops for control of striga hermonthica (del.) benth. in sorghum (sorghum bicolor l. moench) in northern nigeria. | two different field trials (one trial on sorghum intercropped with groundnut [arachis hypogaea l.], and another trial on sorghum intercropped with bambara groundnut [vigna subterranea l. verdc.]) were conducted during the 1995 and 1996 rainy seasons at the teaching and research farm of the department of crop science, faculty of agriculture, university of maiduguri, maiduguri (11 degrees 51' n; 13 degrees 15' e) to evaluate the effect of intercropping resistant and susceptible sorghum varieties w ... | 2002 | 12696409 |
| histochemical characterization of the lectin-binding sites in the equine vomeronasal organ. | the binding specificities of various lectins, such as the dolichos biflorus agglutinin (dba), soybean agglutinin (sba), and the bandeiraea simplicifolia bs-1 (isolectin b4), triticum vulgaris (wga), arachis hypogaea (pna), and ulex europaeus (uea-i) lectins, were studied in the vomeronasal organ of the horse. the microvilli of the vomeronasal sensory epithelium were positive for dba, sba, isolectin b4, wga, pna, and uea-i. the receptor cells showed intense reactivity for dba and wga. lectins wer ... | 2003 | 12819360 |
| histochemical detection of glycoconjugates in the male reproductive system of the horse. | lectins are glycoproteins of plant and animal origin that have the ability to bind specific carbohydrate residues of cell glycoconjugates, particularly in terminal positions. in this study, the binding of lectins, dolichos biflorus agglutinin (dba), soybean agglutinin (sba), bandeiraea simplicifolia bs-1 (isolectin b4), triticum vulgaris (wga), arachis hypogaea (pna), and ulex europaeus (uea-i), was studied in the reproductive systems of male thoroughbred horses.dba was detected in the stereocil ... | 2003 | 12819361 |
| a quantitative evaluation of radiolabelled lectin retention on oral mucosa in vitro and in vivo. | previous work has identified lectins that bind to the cells present on the oral mucosa for their potential use as a means of retaining a drug delivery system on the mucosal surfaces of the mouth. in this study, a radiolabelling technique was developed to allow the quantification of lectin binding to human buccal cells in vitro, and the retention of the lectins in the oral cavity of a rat model in vivo. lectins were labelled with 99mtc using a cyclic diethylene triamine pentaacetic acid conjugati ... | 2002 | 11976016 |
| characterization of a pinus pinaster cdna encoding an auxin up-regulated putative peroxidase in roots. | as part of a study to identify host plant genes regulated by fungal auxin during ectomycorrhiza formation, we differentially screened a cdna library constructed from roots of auxin-treated pinus pinaster (ait.) sol. seedlings. we identified three cdnas up-regulated by auxin. sequence analysis of one of these cdnas, ppprx75, revealed the presence of an open reading frame of 216 amino acids with the characteristic consensus sequences of plant peroxidases. the deduced amino acid sequence showed hom ... | 2002 | 11874719 |
| factors enhancing agrobacterium tumefaciens-mediated gene transfer in peanut (arachis hypogaea l.). | parameters enhancing agrobacterium-mediated transfer of foreign genes to peanut (arachis hypogaea l.) cells were investigated. an intron-containing beta-glucuronidase uida (gusa) gene under the transcriptional control of camv 35s promoter served as a reporter. transformation frequency was evaluated by scoring the number of sectors expressing gus activity on leaf and epicotyl explants. the 'valencia select' market type cv. new mexico was more amenable to agrobacterium transformation than the 'run ... | 1998 | 11760772 |
| evaluation of aqueous moringa seed extract as a seed treatment biofungicide for groundnuts. | in a search for alternatives to currently used fungicides, the potential of aqueous moringa seed extract (amse) as a seed treatment was evaluated. seeds of groundnut, arachis hypogea l cv dakar, were soaked in amse at concentrations of 1, 5, 10, 15 and 20 g litre(-1) for 24 h. comparison was made with apron plus (metalaxyl+carboxin+furathiocarb), until recently a recommended seed-treatment chemical, and distilled water, which was the medium for extraction of moringa seeds. the results showed tha ... | 2003 | 12974360 |
| dissipation of 14c carbaryl and quinalphos in soil under a groundnut crop (arachis hypogaea l.) in semi-arid india. | the dissipation of 14c carbaryl in undisturbed soil cores, and of quinalphos (25ec and 20af) after seed and soil treatments, was investigated under field use conditions, in a semi-arid groundnut field. residues were analyzed by tlc and hplc and additionally by lsc for 14c carbaryl. the harvested seed kernels were also tested for the presence of insecticide residues. the movement of carbaryl was limited to 15 cm depth in the loamy sand of jaipur and was detected till 120 days (dt50 of 14.93 days) ... | 2003 | 14505725 |
| synthesis and protein binding properties of t-antigen containing glycopamam dendrimers. | allyl o-(beta-d-galactopyranosyl)-(1-3)-2-acetamido-2-deoxy-alpha-d-galactopyranoside (8) was prepared in excellent yield from the corresponding galactosyl bromide (6, 7) and allyl 2-acetamido-4,6-benzylidene-2-deoxy-alpha-d-galactopyranoside (5) using hg(cn)2 as a promoter. compound 5 was obtained from n-acetylglucosamine 1 following sequential protecting group strategy and c-4 epimerization as a key step. carboxylic acid functionalized t-antigen derivative 15, obtained by radical addition of 3 ... | 2002 | 11738602 |
| a calcium-dependent bacterial surface protein is involved in the attachment of rhizobia to peanut roots. | as part of a project to characterize molecules involved in the crack-entry infection process leading to nodule development, a microscopic assay was used to visualize the attachment of cells of bradyrhizobium sp. strains semia 6144 and tal 1000 (labelled by introducing a plasmid expressing constitutively the green fluorescent protein gfp-s65t) to arachis hypogaea l. (peanut). qualitative and quantitative results revealed that attachment was strongly dependent on the growth phase of the bacteria. ... | 2003 | 14569294 |
| identification of a cdna clone encoding for a galactose-binding lectin from peanut (arachis hypogaea) seedling roots. | a cdna clone obtained from developing peanut (arachis hypogaea) seedling roots, when expressed in escherichia coli and insect cells (sf9) gave a 29 kda subunit protein. the native recombinant protein agglutinates neuraminidase treated human erythrocytes and the agglutination is inhibited by galactose. nucleotide sequence and predicted amino acid sequence analyses indicate that it is different from peanut seed (pna and sgl) and nodule (ngla and nglb) galactose-binding lectins. | 2001 | 11731092 |
| thomsen-friedenreich (t) antigen: a possible tool for differentiating sebaceous carcinoma from its simulators. | the thomsen-friedenreich (t) antigen is a cryptic glycoprotein, referred to as tumor antigen or cancer-associated antigen because it is absent or masked by some carbohydrates in normal tissues, but present in many human cancers. the latter include gastrointestinal, lung, pancreatic, mammary, and some ovarian carcinomas. cancer cells frequently undergo incomplete glycosylation resulting in the appearance of precursor structures that normally would be absent like the case with the t antigen. t ant ... | 2001 | 11556753 |
| synthesis of n,n'-bis(acrylamido)acetic acid-based t-antigen glycodendrimers and their mouse monoclonal igg antibody binding properties. | novel glycodendrimers based on n,n'-bis(acrylamido)acetic acid core with valencies between two and six were synthesized. the breast cancer-associated t-antigen carbohydrate marker, (beta-gal-(1-3)-alpha-galnac-or), was then conjugated by (i) 1,4-conjugate addition of thiolated t-antigen to the n-acrylamido dendritic cores and by (ii) amide bond formation between an acid derivative of the t-antigen and the polyamino dendrimers. the protein-binding ability of these new glycodendrimers was fully de ... | 2001 | 11456798 |
| mechanisms and diversity of resistance to insect pests in wild relatives of groundnut. | the levels of resistance to insect pests in cultivated groundnut (arachis hypogaea) germplasm are quite low, and therefore, we screened 30 accessions of arachis spp. and 12 derived lines for resistance to insect pests under field and greenhouse conditions. accessions belonging to arachis cardenasii, arachis duranensis, arachis kempff-mercadoi, arachis monticola, arachis stenosperma, arachis paraguariensis, arachis pusilla, and arachis triseminata showed multiple resistance to the leaf miner apro ... | 2003 | 14977130 |
| the effects of the site-directed removal of n-glycosylation from cationic peanut peroxidase on its function. | peanut peroxidase has been diffracted. the location of its heme and calcium moieties have been shown and their role demonstrated. however, the structure and role of its glycans is only now being elucidated. the role of three n-linked complex glycans on cationic peroxidase (cprx) of peanut (arachis hypogaea l cv. valencia), as expressed by prxpnc1 in transgenic tobacco, was analyzed by site-directed replacement of each of the three glycosylation sites, n-60, n-144, and n-185 with q, individually. ... | 2001 | 11360996 |
| monoclonal antibodies against the tn-specific isolectin b4 from vicia villosa seeds: characterization of the epitope of the blocking antibody vv34. | vicia villosa isolectin b4 (vvlb4) recognizes the tn antigen (galnac-o-ser/thr) exposed in certain human carcinomas. we have produced anti-vvlb4 monoclonal antibodies (mabs), and their lectin recognition selectivity was assessed by elisa and western blot against the purified gal/galnac-specific lectins from vicia villosa, salvia sclarea, helix pomatia, arachis hypogaea, glycine max, and dolichos biflorus. the antibodies were also tested for their ability to block the binding of vvlb4 to the tn a ... | 2004 | 15000847 |
| the hydrophobic character of peanut (arachis hypogaea) isoagglutinins. | peanut seed lectin (pna) is widely used to identify tumor-specific antigens on the eukaryotic cell surface. in this work pna was purified by affinity chromatography, using a column containing glutaraldehyde-treated human erythrocytes, whereas pna isoforms were purified by hydrophobic interaction chromatography using phenyl-sepharose. the affinity-purified pna and its isoforms consist of four equal subunits of 24.5 kda each, all of which agglutinated human sialidase-treated erythrocytes equally w ... | 2000 | 11312799 |
| transformation of peanut (arachis hypogaea l.) with tobacco chitinase gene: variable response of transformants to leaf spot disease. | fertile transgenic plants of peanut (arachis hypogaea l.) cv. tmv-2 expressing tobacco chitinase and neomycin phosphotransferase (npt ii) genes were generated using an agrobacterium tumefaciens (lba4404/pbi121-pbtex)-mediated transformation system. a tissue culture-independent method wherein embryo in the mature seed is inoculated and reared into single plant transformant was used for transformation. southern blot analysis of genomic dna isolated from t(0) transformants and progeny plants (t(1)) ... | 2001 | 11297785 |
| effects of ddt on the growth of crop plants. | the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ... | 1989 | 15092369 |
| in vitro capacitation and acrosome reaction of dog spermatozoa can be feasibly attained in a defined medium without glucose. | incubation of dog spermatozoa in a medium without glucose and in the presence of lactate and pyruvate (l-ccm) for 4 h at 38.5 degrees c in a 5% co(2) atmosphere induced in vitro capacitation of these cells. this was verified after the combined specific capacitation-like changes in percentages of viability and altered acrosomes, motility characteristics, sperm location of reactivity against pisum sativum, arachis hypogaea and helix pomatia lectins and the tyrosine phosphorylation pattern. further ... | 2004 | 15182287 |
| segmental expression of somatostatin receptor subtypes sst(1) and sst(2) in tubules and glomeruli of human kidney. | somatostatin is known to modulate mesangial and tubular cell function and growth, but the somatostatin receptor (sst) subtypes responsible for these effects have not been defined. there are at least five different sst receptor subtypes (sst(1)-sst(5)). we used rt-pcr to demonstrate that normal human kidney consistently expresses mrna for sst(1) and sst(2) (9 of 9 donors). some donors expressed sst(4) or sst(5) mrna, but none expressed sst(3) mrna. expression of sst(1) and sst(2) was further asse ... | 2001 | 11181407 |
| systemic and oral immunogenicity of hemagglutinin protein of rinderpest virus expressed by transgenic peanut plants in a mouse model. | rinderpest causes a devastating disease, often fatal, in wild and domestic ruminants. it has been eradicated successfully using a live, attenuated vaccine from most part of the world leaving a few foci of disease in parts of africa, the middle east, and south asia. we have developed transgenic peanut (arachis hypogaea l.) plants expressing hemagglutinin (h) protein of rinderpest virus (rpv), which is antigenically authentic. in this work, we have evaluated the immunogenicity of peanut-expressed ... | 2004 | 15193924 |
| sperm binding to oviductal epithelial cells in the rat: role of sialic acid residues on the epithelial surface and sialic acid-binding sites on the sperm surface. | the aim of this study was to assess the participation of carbohydrate residues in the adhesion of spermatozoa to the oviductal epithelium in the rat. we first examined, by lectin labeling, the distribution of glycoconjugates in rat oviducts obtained under various hormonal environments. several classes of glycoconjugates were abundant in the epithelium, and the expression of some of these molecules varied differentially in ampulla and isthmus, along the estrous cycle and with estradiol and proges ... | 2004 | 15201197 |
| intradermal exposure of balb/c strain mice to peanut protein elicits a type 2 cytokine response. | there is a growing need for the development of methods to characterize the allergenic properties of novel proteins, particularly those expressed by transgenic crop plants. hence, there is considerable interest in the development of suitable animal models for this purpose. the production of specific ige antibody has been reported following sensitization with food allergen via oral or systemic (intraperitoneal) routes of exposure. we have characterized cytokine profiles induced by intradermal trea ... | 2004 | 15304305 |
| an efficient method for the production of transgenic plants of peanut (arachis hypogaea l.) through agrobacterium tumefaciens-mediated genetic transformation. | cotyledon explants from mature peanut seeds (arachis hypogaea l.) were optimized to obtain adventitious shoot buds with high frequencies (>90%). efficient transformation of these cotyledons by using agrobacterium tumefaciens strain c58 carrying neomycin phosphotransferase ii (nptii) and ß-glucuronidase (gus; uida), or coat protein gene of the indian peanut clump virus (ipcvcp) and nptii on binary vectors (pbi121; prokii:ipcvcp) led to the production of a large percentage (55%) of transgenic plan ... | 2000 | 11011088 |
| fortification of ground-nut milk with calcium and its availability to albino rats. | | 1950 | 15421683 |
| altered glycosylation leads to tr polyagglutination. | polyagglutination refers to red blood cells (rbcs) that are agglutinated by a high proportion of abo-matched adult sera but not by cord sera. polyagglutinable rbcs have been associated with microbial infection, myeloproliferative disorders, and myelodysplasia. lectins aid in the identification of polyagglutination. | 2004 | 15504164 |
| [synthesis and degradation of the peanut storage proteins during seed development and germination]. | three polypeptides, 41 kd and 38.5 kd subunits of arachin and 60.5 kd subunit of conarachin in peanut (arachis hypogaea l. shanyou 523) seeds were purified by gel filtration and sds-page. polyclonal antibodies against these subunits were raised in mice. western blot showed that the subunits appeared in axes and cotyledons at the tissue differentiation stage. the 60.5 kd subunit was firstly synthesized and accumulated in considerable quantity in axes and cotyledons, and then the 41 kd and 38.5 kd ... | 2004 | 15583418 |
| growth promotion and yield enhancement of peanut (arachis hypogaea l.) by application of plant growth-promoting rhizobacteria. | although plant growth-promoting rhizobacteria (pgpr) have been reported to influence plant growth, yield and nutrient uptake by an array of mechanisms, the specific traits by which pgpr promote plant growth, yield and nutrient uptake were limited to the expression of one or more of the traits expressed at a given environment of plant-microbe interaction. we selected nine different isolates of pgpr from a pool of 233 rhizobacterial isolates obtained from the peanut rhizosphere on the basis of acc ... | 2004 | 15646384 |
| cyclic related and pathological changes in the lectin-binding sites on the swine oviduct. | this study was carried out to compare the lectin-binding pattern in the normal and pathological oviduct of sows during the ovarian cycle. lectin-binding patterns showed differences between segments, phases of ovarian cycle and presence of morphologic lesions. in the infundibulum, it was observed that the cysts, in the follicular phase, reduced ricinus communis-i (rca-i) and ulex europaeus-i (uea-i) binding. furthermore, in the pathological oviducts of the luteal-phase group, there is a reduction ... | 2005 | 15654999 |
| aflatoxin production in peanut lines selected to represent a range of linoleic acid concentrations. | to determine whether concentrations of linoleate in peanut (arachis hypogaea l.) seed oil could be used to predict an ability to support aflatoxin production, seeds of genotypes representing a range of linoleate content were inoculated with aspergillus flavus link ex fries and assayed for aflatoxin content. seeds were blanched and quartered, inoculated with conidia of a. flavus, placed on moistened filter paper in petri dishes, and incubated for 8 days at 28 degrees c. multiple regression analys ... | 2005 | 15690813 |
| glycosylation of the cationic peanut peroxidase gene expressed in transgenic tobacco. | the major cationic peanut (arachis hypogaea) peroxidase, secreted into the extracellular space, is a glycoprotein with three n-linked glycans (polysaccharides) which are connected to the peptide backbone at asn-60, asn-144 and asn-185. in this report, a c-terminal histidine-tagged cationic peanut peroxidase gene was expressed in transgenic tobacco (nicotiana tabacum). tissue of the transgenic tobacco was cultured in suspension culture and the his-tagged peroxidase was purified in large quantitie ... | 2000 | 10908805 |
| phylloplane bacteria increase seedling emergence, growth and yield of field-grown groundnut (arachis hypogaea l.). | to isolate and characterize groundnut-associated bacterial isolates for growth promotion of groundnut in field. | 2005 | 15752215 |
| the high oleate trait in the cultivated peanut [arachis hypogaea l]. ii. molecular basis and genetics of the trait. | a peanut variety with high oleate content has previously been described. when this high oleate variety was used in breeding crosses, the f2 segregation ratio of high oleate to normal oleate progeny was 3:1 or 15:1 depending on the normal oleate varieties used in the crosses. these data suggested that the high oleate trait is controlled by two recessive genes, and some peanut varieties differ from the high oleate variety by mutations in one gene, while others differ by mutations in two genes. the ... | 2000 | 10905348 |
| enzymatic hydrolysis of luminal coronary glycosidic structures uncovers their role in sensing coronary flow. | endothelial luminal glycocalyx (elg) is a multifunctional complex structure made off of a diversity of glycosilated proteins, and glycosaminoglycans (gag). coronary elg may participate as a sensor of coronary flow (cf) to induce inotropic and dromotropic effects. in isolated perfused guinea pig heart we tested the role of glycosidic groups of glycans bound to proteins and gag of the elg on cf-induced inotropic and dromotropic effects. to study the role of saccharide related groups of certain gly ... | 2005 | 15769604 |
| mammalian-like nonsialyl complex-type n-glycosylation of equine gonadotropins in mimic insect cells. | recombinant equine luteinizing hormone/chorionic gonadotropin (elh/cg) was expressed in mimic insect cells, that are commercial stably transformed spodoptera frugiperda (sf9) cells expressing five mammalian genes encoding glycosyltransferases involved in the synthesis of complex-type monosialylated n-glycans. we previously showed that it exhibited no in vivo bioactivity although expressing full in vitro bioactivity, and it was suspected that this was because of insufficient sialylation of elh/cg ... | 2005 | 15814822 |
| sandwich immunoassays for the determination of peanut and hazelnut traces in foods. | people suffering from food allergies are dependent on accurate food labeling, as an avoidance diet is the only effective countermeasure. even a small amount of allergenic protein can trigger severe reactions in highly sensitized patients. therefore, sensitive and reliable tests are needed to detect potential cross-contamination. in this paper two fast sandwich immunoassays are described for the determination of peanut (arachis hypogaea) and hazelnut (corylus avellana) traces in complex food matr ... | 2005 | 15853366 |
| dehydrogenase and phosphomonoesterase activities in groundnut (arachis hypogaea l.) field after diazinon, imidacloprid and lindane treatments. | impacts of diazinon, imidacloprid and lindane treatments on dehydrogenase and alkaline phosphomonoesterase enzyme activities were determined in groundnut (arachis hypogaea l.) field for three consecutive years (1997-1999). diazinon was applied as both seed and soil treatments but imidacloprid and lindane were used for seed treatments only at recommended rates. experiments were conducted at agricultural research station durgapura, jaipur, rajasthan, india. diazinon residues were persist up till 6 ... | 2005 | 15910899 |
| determination of the phytoalexin resveratrol (3,5,4'-trihydroxystilbene) in peanuts and pistachios by high-performance liquid chromatographic diode array (hplc-dad) and gas chromatography-mass spectrometry (gc-ms). | the phytoalexin resveratrol (3,5,4'-trihydroxystilbene) in edible peanut (arachis hypogaea l.) and pistachio (pistacia vera l.) varieties grown in turkey was analyzed by high-performance liquid chromatographic diode array and gas chromatography-mass spectrometric detection. trans-resveratrol in six peanut varieties, five pistachio varieties, and four market samples ranged between 0.03 and 1.92 microg/g. the cerezlik 5025 peanut (1.92 +/- 0.01 microg/g) and ohadi pistachio genotype (1.67 +/- 0.01 ... | 2005 | 15941348 |
| endothelial glycoconjugates: a comparative lectin study of the brain, retina and myocardium. | there is evidence that the endothelial cell (ec) glycocalyx is a significant determinant of vascular permeability, acting as a charge-size filter to permeant molecules. we have therefore examined its oligosaccharide composition in 3 classes of microvessel with differing permeabilities. ec in rat brain, retina and myocardium were labelled with a panel of lectins and subjected to a semiquantitative analysis. surprisingly, no substantial differences were evident for any lectin labelling between the ... | 2000 | 10697288 |
| synthesis of bioadhesive lectin-hpma copolymer-cyclosporin conjugates. | an amino group containing cyclosporin a (csa) derivative has been synthesized and conjugated to n-(2-hydroxypropyl)methacrylamide (hpma) copolymer via an aromatic azo bond, which can be specifically cleaved by azoreductase activity in colon to release the drug for the treatment of colon diseases. lectins, peanut (arachis hypogea) agglutinin (pna) and wheat germ agglutinin (wga), have been conjugated to hpma copolymer-csa derivative conjugates (pcsa), respectively, to give bioadhesive conjugates. ... | 2000 | 10639078 |
| influence of palm olein on protein utilisation in the growing rat. | in view of our preliminary findings that refined palm oil (rpol) enhanced protein utilisation, and because of the growing importance of palm oil in human diets, two separate studies were conducted. the first study aimed to investigate the effect of the palm oil source and fraction on protein utilisation, as determined by net protein utilization (npu) in growing rats. the second study aimed to investigate the influence of the dietary concentration of one particular palm oil fraction, refined palm ... | 1999 | 10616647 |
| indirect competitive elisa for determination of traces of peanut (arachis hypogaea l.) protein in complex food matrices. | an indirect competitive elisa was developed allowing the detection of hidden peanut protein residues down to 2 ppm (micorgrams per gram) in various foods. the high-titer, peanut-specific polyclonal antiserum used recognized potentially allergenic proteins in both native and roasted peanuts. in the absence of a food matrix, extractable protein from roasted peanuts was detected at 104 +/- 13%. from various food items, peanut protein at > or =13 ppm was recovered between 84 and 126%, and at 2 ppm o ... | 1999 | 10563939 |
| identification and characterization of phospholipase d and its association with drought susceptibilities in peanut (arachis hypogaea). | preharvest aflatoxin contamination has been identified by the peanut industry as a serious issue in food safety and human health because of the carcinogenic toxicity. drought stress is the most important environmental factor exacerbating aspergillus infection and aflatoxin contamination in peanut. the development of drought-tolerant peanut cultivars could reduce aflatoxin contamination and would represent a major advance in the peanut industry. in this study, we identified a novel pld gene in pe ... | 2006 | 16200410 |
| aflatoxin production in six peanut (arachis hypogaea l.) genotypes infected with aspergillus flavus and aspergillus parasiticus, isolated from peanut production areas of cordoba, argentina. | aflatoxin contamination is one of the main factors affecting peanut seed quality. one of the strategies to decrease the risk of peanut aflatoxin contamination is the use of genotypes with resistance to aspergillus infection. this laboratory study reports the resistance to aspergillus infection and aflatoxin contamination of six peanut genotypes inoculated with 21 aspergillus isolates obtained from the peanut production region of cordoba, argentina. the resistance was investigated in the seed coa ... | 2005 | 16277433 |
| cross-reaction of chalcone synthase and stilbene synthase overexpressed in escherichia coli. | chalcone synthase (chs) and stilbene synthase (sts) are related plant polyketide synthases belonging to the chs superfamily. chs and sts catalyze common condensation reactions of p-coumaroyl-coa and three c(2)-units from malonyl-coa but different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. using purified pueraria lobata chs and arachis hypogaea sts overexpressed in escherichia coli, bisnoryangonin (bny, the derailed lactone after two condensations) and p-c ... | 1999 | 10556516 |
| presence of vicia graminea or vicia unijuga lectin-binding (vgu) glycoproteins with and without thomsen-friedenreich (t) antigen in normal human seminal plasma. | vgu glycoprotein (vicia graminea lectin- or vicia unijuga lectin-binding glycoprotein) has been reported as oncofetal antigen, which is found in many kind of tumor tissues, amniotic fluid and fetal membranes. in autoradiography with an 125i-labeled vicia unijuga lectin (vua) probe and an 125i-labeled arachis hypogaea lectin (pna, anti-t lectin) probe, seminal plasma samples of eight healthy men gave 2-7 vgu glycoproteins without t antigen, 1-2 vgu glycoproteins with thomsen-friedenreich antigen ... | 2001 | 12889521 |
| oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses. | rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. global eradication of rinderpest needs a robust, safe and cost-effective vaccine. the causative agent, rinderpest virus (rpv) is an important member of the genus morbillivirus in the paramyxoviridae family. we have generated transgenic peanut (arachis hypogea l.) plants expressing hemagglutinin protein of rpv and report here, the induction of immune responses in cattle following or ... | 2003 | 12804859 |