| nondestructive application of laser-induced fluorescence spectroscopy for quantitative analyses of phenolic compounds in strawberry fruits (fragaria x ananassa). | laser-induced fluorescence spectroscopy (lifs) was nondestructively applied on strawberries (ex = 337 nm, em = 400-820 nm) to test the feasibility of quantitatively determining native phenolic compounds in strawberries. eighteen phenolic compounds were identified in fruit skin by uv and ms spectroscopy and quantitatively determined by use of rp-hplc for separation and diode-array or chemical reaction detection. partial least-squares calibration models were built for single phenolic compounds by ... | 2008 | 18416555 |
| pectin esterase gene family in strawberry fruit: study of fape1, a ripening-specific isoform. | pectin esterases (pe, ec 3.1.1.11) catalyse the demethylation of pectin. as a result of its activity, structural interactions among cell wall components during cell wall turnover and loosening are affected. in plants, pes are typically encoded by a gene family. this family has been studied in strawberry (fragaria x ananassa duch.) in order to investigate the role of distinct pe genes during fruit ripening and senescence. by a combination of a pcr-based library screening and rt-pcr four different ... | 2004 | 15020638 |
| cloning and partial characterization of a beta-1,3-glucanase gene from strawberry. | plant pathogenesis-related proteins, including beta-1,3-glucanses, are believed to be involved in plant defense mechanisms. we have cloned a beta-1,3-glucanse gene from strawberry (fragaria x ananassa duch). this gene, designated fabg2-1, is a class ii glucanase gene composed of two exons and one intron. the location of a 397-base intron in the gene was confirmed by sequencing a partial cdna clone obtained by using a rapid amplification of cdna ends procedure. also, based on the cdna sequence, t ... | 2003 | 15018349 |
| the development of a bin mapping population and the selective mapping of 103 markers in the diploid fragaria reference map. | we have identified a set of plants (the bin set) to permit "selective" or "bin" mapping using the diploid strawberry mapping population fv x fn, derived from the f2 cross f. vesca 815 x f. nubicola 601, which has been used to develop the fragaria reference map. the bin set consists of 8 plants: the f. vesca 815 parent, the f1 hybrid individual, and 6 seedlings of the f2 population. this bin set divides the 578 cm of the diploid fragaria genome into 46 bins, the largest mapping bin being 26 cm in ... | 2008 | 18356946 |
| transgenic strawberry: state of the art for improved traits. | strawberry (fragaria x ananassa duch.), a member of the rosaceae family, is one of the most important fruit crops cultivated worldwide. strawberry is unique within the rosaceae because it is a rapidly growing herbaceous perennial with a small genome, short reproductive cycle, and facile vegetative and generative propagation for genetic transformation. for these reasons, strawberry has been recognized as excellent germplasm for genetic and molecular studies for the rosaceae family. although tradi ... | 2008 | 18280082 |
| [spatial distribution of phytophagous mites (acari: tetranychidae) on strawberry plants]. | many phytophagous mites can attack strawberry plants, fragaria x ananassa, among them the southern red mite, oligonychus ilicis mcgregor, and the two-spotted spider mite, tetranychus urticae koch. they are found together feeding on the same plant on the upper and underside of the leaves, respectively. here we studied the choice for feeding sites of o. ilicis and t. urticae on strawberry plants. the first hypothesis tested whether the feeding site choice would be related to the fitness of the spe ... | 2007 | 18060306 |
| isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (fragaria x ananassa duch.). | strawberry (fragaria spp.) is a kind of herbaceous perennial plant that propagates vegetatively. the conserved domains of reverse transcriptase (rt) genes of ty1-copia and ty3-gypsy groups of ltr retrotransposons were amplified from the cultivated strawberry (fragaria x ananassa duch.). sequence analysis of clones demonstrated that 5 of 19 ty1-copia group unique sequences and 2 of 10 ty3-gypsy unique sequences in f. x ananassa genome possessed either stop codon or frameshift. ty1-copia group seq ... | 2008 | 18026732 |
| apoplastic antioxidative system responses to ozone stress in strawberry leaves. | cell wall polysaccharides, pectin composition, as well as apoplastic superoxide dismutase and peroxidase activities were investigated in strawberry (fragaria x ananassa) cultivars (cvs) korona and elsanta differing in their ozone sensitivity. plants were exposed to 140-170 microg m(-3) ozone either short-term for 7 days or long-term for 2 months in order to investigate whether differences in ozone sensitivity were due to differences in the apoplastic antioxidative systems. cell wall polysacchari ... | 2008 | 17913290 |
| resveratrol content in strawberry fruit is affected by preharvest conditions. | this study investigated the occurrence of resveratrol in fragaria x ananassa duchesne and the effect of preharvest conditions on resveratrol content. both cis- and trans-resveratrol were detected in strawberry achenes (seeds) and pulp (receptacle tissue). resveratrol was found to be higher in achenes than in fruit pulp. the levels of resveratrol were affected by genotype variations, fruit maturation, cultural practices, and environmental conditions. high growing temperature (25 and 30 degrees c) ... | 2007 | 17803264 |
| characterization of phenolic compounds in strawberry (fragaria x ananassa) fruits by different hplc detectors and contribution of individual compounds to total antioxidant capacity. | phenolic compounds in strawberry (fragaria x ananassa) fruits were identified and characterized by using the complementary information from different high-performance liquid chromatography detectors: diode array, mass spectrometer in positive and negative mode, and coulometric array. electrochemical profiles obtained from the coulometric array detector contributed to the structural elucidation suggested from the uv-vis and mass spectra. about 40 phenolic compounds including glycosides of quercet ... | 2007 | 17472391 |
| fagt2: a multifunctional enzyme from strawberry (fragaria x ananassa) fruits involved in the metabolism of natural and xenobiotic compounds. | fragaria x ananassa udp-glucose:cinnamate glucosyltransferase (fagt2) catalyzes the formation of cinnamic acid and p-coumaric acid glucose esters during strawberry fruit ripening. here, the ripening and oxidative stress induced enzyme was further characterized by testing a range of structurally different substrates of natural and unnatural origin in vitro and comparing their kinetic parameters to elucidate its additional biological functions. the accepted substrates ranged from derivatives of ci ... | 2007 | 17323078 |
| assessment of the differences in the phenolic composition of five strawberry cultivars (fragaria x ananassa duch.) grown in two different soilless systems. | the phenolics from different strawberry cultivars (aromas, camarosa, diamante, medina, and ventana) cultivated in two different soilless systems (with and without recycling nutrient solution) were quantified to assess differences in their profiles as a function of both the variety and the cultivation system. considering groups of phenols, it was found that either anthocyanins (including pelargonidin-3-glucoside, cyanidin-3-glucoside, pelargonidin-3-rutinoside, pelargonidin-3-acetylglucoside, and ... | 2007 | 17279770 |
| lipoxygenase involvement in ripening strawberry. | the enzymatic activity, subcellular localization, and immunolocalization of plant lipoxygenase (lox) in strawberry fruits (fragaria x ananassa, duch) were investigated. chemical and enzymatic properties of lox have been characterized, and the lox capability of oxygenating free and esterified unsaturated fatty acids into c6 volatile aldehydes has been confirmed. fruits at unripe, turning, and ripe stages were analyzed for lox activity and protein localization by western blots, two-dimensional ele ... | 2006 | 16939347 |
| stable isotope and trace element profiling combined with classification models to differentiate geographic growing origin for three fruits: effects of subregion and variety. | classifications of geographic growing origin of three fresh fruits combining elemental profiles with various modeling approaches were determined. elemental analysis (ca, cd, cr, cu, fe, k, mg, mn, na, ni, p, v, and zn) of strawberry, blueberry, and pear samples was performed using inductively coupled plasma argon atomic emission spectrometer. bulk stable carbon and nitrogen isotope analyses in pear were performed using mass spectrometry as an alternative fingerprinting technique. each fruit, str ... | 2006 | 16786991 |
| identification of a zinc transporter gene in strawberry. | we have cloned a zrt (zn-regulated transporter), irt (fe-regulated transporter)-like protein (zip) gene from the strawberry plant (fragaria x ananassa duch). this gene, designated as fazip1, is composed of three exons and two introns. the locations of the two introns (546 and 157 base pairs) in the gene were confirmed by sequence analysis of cdna clones obtained by using 3' and 5' rna ligase mediated-rapid amplification of cdna ends (rlm-race) pcr. also, based on the cdna sequence, the transcrip ... | 2006 | 16753813 |
| influence of fertilization, mulch color, early forcing, fruit order, planting date, shading, growing environment, and genotype on the contents of selected phenolics in strawberry (fragaria x ananassa duch.) fruits. | the influence of agricultural practices (fertilization, mulch color, early forcing, and planting date), environment (light and growing area), cultivar, and fruit order on the selected phenolic content and antioxidant activity in strawberry (fragaria x ananassa duch.) fruits was studied. three different levels of fertilization were given to plants in the fertilization experiment. the lowest fertilization level increased the contents of flavonols and ellagic acid from 19 to 57%. between cultivars, ... | 2006 | 16569052 |
| tdz, auxin and genotype effects on leaf organogenesis in fragaria. | the different types of organogenic (roots and adventitious shoots) and callus formation responses of leaves from 30-day-old proliferating shoots of different fragaria spp. genotypes were studied in response to ms medium supplemented with 4.54 microm 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron; tdz) alone and in combination with 0.98 microm indole-3-butyric acid (iba), 0.84 microm 3-benzo[b]selenienyl acetic acid (bsaa) or 0.90 microm 2,4-dichlorophenoxy acetic acid (2,4-d). the study in ... | 2006 | 16231183 |
| seasonal patterns of photosynthetic response and acclimation to elevated carbon dioxide in field-grown strawberry. | strawberry (fragaria x ananassa) plants were grown in field plots at the current ambient [co(2)], and at ambient + 300 and ambient + 600 mumol mol(-1) [co(2)]. approximately weekly measurements were made of single leaf gas exchange of upper canopy leaves from early spring through fall of two years, in order to determine the temperature dependence of the stimulation of photosynthesis by elevated [co(2)], whether growth at elevated [co(2)] resulted in acclimation of photosynthesis, and whether any ... | 2001 | 16228346 |
| in vitro antileukaemic activity of extracts from berry plant leaves against sensitive and multidrug resistant hl60 cells. | the aim of the present study was to determine in vitro antileukaemic activity of extracts obtained from selected berry plant leaves (fragaria x ananassa duch. cv elsanta, raspberry rubus ideus l. cv polana and blueberry vaccinium corymbosum l. cv bluecrop) against promyelocytic hl60 cell line and its multidrug resistant sublines exhibiting two different mdr phenotypes: hl60/vinc (overexpressing p-glycoprotein) and hl60/dox (overexpressing mrp1 protein). it was found that the blueberry extract wa ... | 2006 | 16039042 |
| expressed sequence tags (ests) and simple sequence repeat (ssr) markers from octoploid strawberry (fragaria x ananassa). | cultivated strawberry (fragaria x ananassa) represents one of the most valued fruit crops in the united states. despite its economic importance, the octoploid genome presents a formidable barrier to efficient study of genome structure and molecular mechanisms that underlie agriculturally-relevant traits. many potentially fruitful research avenues, especially large-scale gene expression surveys and development of molecular genetic markers have been limited by a lack of sequence information in pub ... | 2005 | 15985176 |
| genetic variance and breeding values for resistance to a wind-borne disease [sphaerotheca macularis (wallr. ex fr.)] in strawberry (fragaria x ananassa duch.) estimated by exploring mixed and spatial models and pedigree information. | a mixed model approach was used to estimate variance components and heritabilities for resistance to powdery mildew, a wind-borne disease in strawberry. in order to improve precision in the statistical computations, spatial error control effects were included to account for systematic environmental variations in the large field trials. pedigree information was included where feasible. seedling families obtained from an incomplete 63-by-63 diallel cross were grown at six locations and scored subj ... | 2005 | 15937703 |
| caps markers improved by cluster-specific amplification for identification of octoploid strawberry (fragaria x ananassa duch.) cultivars, and their disomic inheritance. | cleavage amplified polymorphic sequence (caps) markers of strawberry (fragaria x ananassa duch.) can be useful for identifying mislabeled or patent-infringing cultivars in the marketplace. however, caps markers in octoploid strawberry tend to give unclear bands because multiple homologous sites are simultaneously amplified by the non-selective pcr. to overcome this problem, we used "cluster-specific amplification" based on the nucleotide sequences of pcr products and were able to improve the ban ... | 2005 | 15843913 |
| apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods. | this paper presents results from a study to develop cryopreservation procedures for apices of several strawberry genotypes. five fragaria x ananassa duch. cultivars and two wild species (f. chiloensis and f. virginiana) have been screened using the encapsulation-dehydration method and/or a protocol which compromises vitrification and encapsulation-dehydration. apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 m, conventional protocol), or a ... | 2009 | 15772709 |
| a strawberry fruit-specific and ripening-related gene codes for a hyprp protein involved in polyphenol anchoring. | a strawberry (fragaria x ananassa cv. chandler) fruit cdna (fahyprp -cdna) and its corresponding gene (fahyprp) showing sequence homology to higher plant hyprp genes have been isolated. the cdna contains an open reading frame encoding a 16 kda protein with 156 amino acids. the peptide has an amino-terminal signal sequence, a repetitive proline-rich sequence, and a cysteine-rich carboxy-terminal region homologous to other hyprp proteins. northern blot and qrt-pcr analysis have shown that the stra ... | 2004 | 15604715 |
| changes in cell wall composition of three fragaria x ananassa cultivars with different softening rate during ripening. | fleshy fruit soften during ripening mainly as a consequence of solubilization and depolymerization of cell wall components. we have performed a comparative study of the polysaccharide content of fruit cell walls during final steps of development and ripening of three strawberry (fragaria x ananassa duch.) cultivars with different softening rates. the three chosen varieties showed very different firmness; camarosa was the firmest, toyonaka the softest, and pajaro intermediate between them. cell w ... | 2004 | 15596102 |
| antioxidants, low molecular weight carbohydrates, and total antioxidant capacity in strawberries (fragaria x ananassa): effects of cultivar, ripening, and storage. | four cultivars of strawberries (senga sengana, bfr77111, elsanta, and honeoye) were studied for their content of antioxidants, total antioxidant capacity, and low molecular weight carbohydrates in relation to harvest year, ripening stage, and cold storage. for ascorbic acid, chlorogenic acid, ellagic acid, and total antioxidative capacity, measured in both water-soluble and water-insoluble extracts, there was a 2-5-fold variation among cultivars. unripe berries contained lower concentrations of ... | 2004 | 15113146 |
| identification, cloning and expression analysis of strawberry (fragaria x ananassa) mitochondrial citrate synthase and mitochondrial malate dehydrogenase. | salt-extractable proteins from the cell walls of immature and ripe strawberry (fragaria x ananassa duch. cv. elsanta) fruit were separated using two-dimensional polyacrylamide gel electrophoresis. seven polypeptides (enzymes) were characterized from their n-terminal sequences: (1) glyceraldhyde-3-phosphate dehydrogenase (ec 1.2.1.12); (2) triose phosphate isomerase (tpi; ec 5.3.1.1); (3) mitochondrial malate dehydrogenase (mmdh; ec 1.1.1.37); (4) nadh glutamate dehydrogenase (ec 1.4.1.3); (5) ch ... | 2004 | 15086813 |
| adventitious shoot regeneration from seven commercial strawberry cultivars (fragaria x ananassa duch.) using a range of explant types. | the parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars calypso, pegasus, bolero, tango and emily were achieved with leaf discs, petioles, roots and stipules. regeneration from cv. elsanta proved to be difficult from all explant material, although unpollinated ovaries proved to be a promising explant sour ... | 2003 | 12789440 |
| characterization of mixed disomic and polysomic inheritance in the octoploid strawberry (fragaria x ananassa) using aflp mapping. | a two-way pseudo-testcross strategy, combined with single dose restriction fragment (sdrf) marker analysis, was used for genetic mapping in the octoploid cultivated strawberry fragaria x ananassa (2n = 8 x = 56). based on a 113 full-sib progeny from a cross between the variety capitola and the clone cf1116, we generated two parental maps using amplified fragment length polymorphism (aflp) markers. ninety two percent of the markers (727 out of 789) showed ratios corresponding to simplex markers ( ... | 2003 | 12768242 |
| detection of phloridzin in strawberries (fragaria x ananassa duch.) by hplc-pda-ms/ms and nmr spectroscopy. | the phenolic profile of strawberry fruits (fragaria x ananassa duch., rosaceae) was investigated by high-performance liquid chromatography with photodiode array detection. a peak displaying retention time and uv spectral data identical to those of phloridzin (phloretin 2'-o-beta-d-glucoside), a dihydrochalcone glucoside so far considered characteristic of apples, was monitored. for further characterization, crude extracts of strawberries were purified on polyamide, and the target compound was is ... | 2003 | 12720368 |
| cloning and characterization of two ripening-related strawberry (fragaria x ananassa cv. chandler) pectate lyase genes. | two genomic clones corresponding to putative pectate lyase genes (pla and plb) were isolated and characterized in strawberry (fragaria x ananassa cv. chandler). the corresponding orfs for the pla and plb genes revealed deduced proteins of 451 and 439 amino acids, respectively, that differ from that of the previously isolated strawberry plc gene. southern blot analysis has shown that while the plb gene is a single copy gene, the pla gene is probably encoded by a small multigene family. by using s ... | 2003 | 12554706 |
| folate content in strawberries (fragaria x ananassa): effects of cultivar, ripeness, year of harvest, storage, and commercial processing. | folate concentrations in strawberries and folate retention during storage and commercial processing of strawberries were investigated. no previous study has focused on the effects of cultivar, ripeness, and year of harvest of strawberries with respect to the folate content. this study showed the folate concentration in strawberries to significantly depend on all of these different factors. total folate was quantified using a modified and validated radioprotein-binding assay with external calibra ... | 2003 | 12502396 |
| comparison of economic and plant phenology-based thresholds for management of lygus lineolaris (hemiptera: miridae) in minnesota strawberries. | field studies were conducted in southeastern minnesota, 2000-2002, to assess damage potential and management options for adult lygus lineolaris (palisot de beauvois) in june-bearing strawberries (fragaria x ananassa). the first study was designed to assess the efficacy of a published economic threshold for l. lineolaris nymphs compared with a plant phenology-based threshold management program, targeted at l. lineolaris adults. l. lineolaris nymphs were sampled using the standard white pan beat m ... | 2003 | 14650524 |
| compost as a soil supplement increases the level of antioxidant compounds and oxygen radical absorbance capacity in strawberries. | compost as a soil supplement significantly enhanced levels of ascorbic acid (asa) and glutathione (gsh) and ratios of asa/dehydroascorbic acid (dhasa) and gsh/oxidized glutathione (gssg) in fruit of two strawberry (fragaria x ananassa duch.) cultivars, allstar and honeoye. the peroxyl radical (roo(*)) as well as the superoxide radical (o(2)(*)(-)), hydrogen peroxide (h(2)o(2)), hydroxyl radical (oh(*)), and singlet oxygen ((1)o(2)) absorbance capacity in strawberries increased significantly with ... | 2003 | 14582984 |
| elevated carbon dioxide increases contents of antioxidant compounds in field-grown strawberries. | the effects of elevated co2 concentrations on the antioxidant capacity and flavonoid content in strawberry fruit (fragaria x ananassa duch.) were studied under field conditions. increased co(2) (300 and 600 micromol mol(-1) above ambient) concentrations resulted in increases in ascorbic acid (asa), glutathione (gsh), and ratios of asa to dehydroascorbic acid (dhasa) and gsh to oxidized glutathione (gssg), and a decrease in dhasa in strawberry fruit. high anthocyanin and phenolic content were als ... | 2003 | 12848504 |
| fruit plant germplasm characterisation using molecular markers generated in rapd and issr-pcr. | the genotypes of the strawberry (fragaria x ananassa), apple (malus domestica) and ribes species (r. nigrum, r. rubrum and r. glossularia), maintained in our institute's collection and used in breeding programs, were screened for dna markers. twenty primers for rapd (among 60 tested) and seven for issr (among 10 tested) were chosen as creating polymorphic dna bands differentiating the investigated genotypes. based on those identity markers, the genetic distance between genotypes was determined, ... | 2002 | 12378239 |
| novel insight into vascular, stress, and auxin-dependent and -independent gene expression programs in strawberry, a non-climacteric fruit. | using cdna microarrays, a comprehensive investigation of gene expression was carried out in strawberry (fragaria x ananassa) fruit to understand the flow of events associated with its maturation and non-climacteric ripening. we detected key processes and novel genes not previously associated with fruit development and ripening, related to vascular development, oxidative stress, and auxin response. microarray analysis during fruit development and in receptacle and seed (achene) tissues establishe ... | 2002 | 12114557 |
| comparative study of the products of the peroxidase-catalyzed and the polyphenoloxidase-catalyzed (+)-catechin oxidation. their possible implications in strawberry (fragaria x ananassa) browning reactions. | the peroxidase- and polyphenoloxidase-catalyzed oxidations of (+)-catechin yield several products showing different degrees of polymerization, which are apparently responsible for the pigment decay and the associated browning reaction that occurs in processed strawberry fruits and their derived foods. in this work, we have purified both peroxidase and polyphenoloxidase from oso grande cv. strawberry fruits, and comparatively analyzed the products of their enzyme-mediated (+)-catechin oxidation. ... | 2002 | 11853507 |
| volatile compounds of selected strawberry varieties analyzed by purge-and-trap headspace gc-ms. | six strawberry (fragaria x ananassa duch.) varieties, senga sengana, jonsok, polka, korona, bounty, and honeoye, were studied in relation to composition of their volatile headspace compounds collected by dynamic purge-and-trap concentration. also, the influence of cultivation technique on the volatile compounds was tested by comparing conventionally and organically cultivated strawberry varieties. in addition, the effect of geographical origin on strawberry volatiles was investigated. the emphas ... | 2002 | 11853494 |
| antioxidant capacity, vitamin c, phenolics, and anthocyanins after fresh storage of small fruits. | fresh strawberries (fragaria x ananassa duch.), raspberries (rubus idaeus michx.), highbush blueberries (vaccinium corymbosum l.), and lowbush blueberries (vaccinium angustifolium aiton) were stored at 0, 10, 20, and 30 degrees c for up to 8 days to determine the effects of storage temperature on whole fruit antioxidant capacity (as measured by the oxygen radical absorbing capacity assay, cao et al., clin. chem. 1995, 41, 1738-1744) and total phenolic, anthocyanin, and ascorbate content. the fou ... | 1999 | 10552863 |
| purification and properties of an endo-beta-1,4-glucanase from strawberry and down-regulation of the corresponding gene, cel1. | an endo-beta-1,4-glucanase (eg) was purified from ripe strawberry (fragaria x ananassa duch.) fruit using cellulose affinity chromatography. the purified enzyme gave a single protein band of 54 kda on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the n-terminal amino acid sequence of this protein showed strong homology with the proteins encoded by recently identified eg genes from different strawberry cultivars and from arabidopsis, pepper and tomato. the enzyme specifically cleave ... | 2001 | 11762160 |
| cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries. | anthocyanins from tart cherries, prunus cerasus l. (rosaceae) cv. balaton and montmorency; sweet cherries, prunus avium l. (rosaceae); bilberries, vaccinum myrtillus l. (ericaceae); blackberries, rubus sp. (rosaceae); blueberries var. jersey, vaccinium corymbosum l. (ericaceae); cranberries var. early black, vaccinium macrocarpon ait. (ericaceae); elderberries, sambucus canadensis (caprifoliaceae); raspberries, rubus idaeus (rosaceae); and strawberries var. honeoye, fragaria x ananassa duch. (ro ... | 2001 | 11695879 |
| antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. | fruits and leaves from different cultivars of thornless blackberry (rubus sp.), red raspberry (rubus idaeus l.), black raspberry (rubus occidentalis l.), and strawberry (fragaria x ananassa d.) plants were analyzed for total antioxidant capacity (oxygen radical absorbance capacity, orac) and total phenolic content. in addition, fruits were analyzed for total anthocyanin content. blackberries and strawberries had the highest orac values during the green stages, whereas red raspberries had the hig ... | 2000 | 10691606 |
| effect of plant growth temperature on antioxidant capacity in strawberry. | the influence of four day/night growing temperature combinations (18/12, 25/12, 25/22, and 30/22 degrees c) on phenolic acid, flavonol, and anthocyanin content and their antioxidant activities against peroxyl radicals (roo(*)), superoxide radicals (o(2)(*)(-)), hydrogen peroxide (h(2)o(2)), hydroxyl radicals (oh(*)), and singlet oxygen ((1)o(2)) in fruit juice of earliglow and kent strawberry (fragaria x ananassa duch.) cultivars was studied. pelargonidin-based anthocyanins such as pelargonidin ... | 2001 | 11600054 |
| a fruit-specific and developmentally regulated endopolygalacturonase gene from strawberry (fragaria x ananassa cv. chandler). | a fruit-specific and developmentally regulated polygalacturonase gene (spg gene) from strawberry (fragaria x ananassa cv. chandler) has been cloned and characterized at a molecular and physiological level. comparison analysis of the corresponding deduced spg protein have shown that this strawberry gene is similar to clade a endopolygalacturonase genes. moreover, the spatio-temporal and hormonal gene expression pattern suggests a close relationship between the expression of this gene and the onse ... | 2001 | 11520883 |
| an expansin gene expressed in ripening strawberry fruit. | tissue softening accompanies the ripening of many fruit and initiates the processes of irreversible deterioration. expansins are plant cell wall proteins proposed to disrupt hydrogen bonds within the cell wall polymer matrix. expression of specific expansin genes has been observed in tomato (lycopersicon esculentum) meristems, expanding tissues, and ripening fruit. it has been proposed that a tomato ripening-regulated expansin might contribute to cell wall polymer disassembly and fruit softening ... | 1999 | 10594114 |
| effects of ozone treatment on postharvest strawberry quality. | the effect of ozone treatment on the postharvest quality of strawberry was evaluated. strawberry fruits (fragaria x ananassa duch. cv. camarosa) were stored at 2 degrees c in an atmosphere containing ozone (0.35 ppm). after 3 days at 2 degrees c, fruits were moved to 20 degrees c to mimic retail conditions (shelf life). the changes in several quality parameters such as fungal decay, color, sugar and acids distribution, and aroma were evaluated during the strawberries' shelf life. ozone treatment ... | 1999 | 10564033 |
| carbohydrate differences in strawberry crowns and fruit (fragaria x ananassa) during plant development. | carbohydrates accumulation and mobilization are highly relevant in plants because they have been related to yield and quality. therefore, the aims of this work were to determine soluble carbohydrates and starch in strawberry (fragaria x ananassa cv. camarosa) crown sections (basal, middle, and upper) at three different plant growth stages (vegetative, blooming, and fruiting), and in fruit varieties (cv. camarosa, seascape, and oso grande) grown on the same field and in a different geoclimate. th ... | 2002 | 12010004 |
| cloning and sequence determination of a gene encoding an osmotin-like protein from strawberry (fragaria x ananassa duch.). | osmotin and osmotin-like proteins (olps) are pathogenesis-related (pr) proteins, whose synthesis is normally stimulated upon infection of plants by pathogens. a strawberry genomic clone containing an osmotin-like protein (olp) gene was isolated and sequenced. this clone contains an open reading frame of 681 nucleotides without any intron. the predicted amino acid sequence of the protein shares high degrees of homology with a number of other olps and related proteins, of which several are known t ... | 2001 | 11913795 |
| metabolic fate of isotopes during the biological transformation of carbohydrates to 2,5-dimethyl-4-hydroxy-3(2h)-furanone in strawberry fruits. | isotopically labeled d-glucose, d-fructose, 1-deoxy-d-fructose, and 6-deoxyhexoses were applied to detached ripening strawberry (fragaria x ananassa) fruits, and the incorporation of the isotopes into the key strawberry aroma compounds 2,5-dimethyl-4-hydroxy-3(2h)-furanone (dmhf, 1) and 2,5-dimethyl-4-methoxy-3(2h)-furanone (dmmf, 2) was determined by gas chromatography-mass spectrometry. in contrast to previous reports the data clearly showed that 6-deoxy-d-fructose/6-deoxy-d-glucose and 1-deox ... | 2001 | 11368615 |
| cytosolic aldolase is a ripening related enzyme in strawberry fruits (fragaria x ananassa). | two aldolase isoenzymes have been isolated from ripe strawberry fruits (fragaria x ananassa cv. camarosa and elsanta) and partially purified by deae anion exchange and sephacryl size exclusion chromatography. the isoenzymes were identified as class i cytosol and plastid aldolase on the basis of their chromatographic behavior on deae-cellulose columns, native molecular weight, ph optimum pattern, km value for d-fructose-1,6-bisphosphate, tendency to be inactivated by lower ph values and sds-page ... | 2001 | 11261572 |
| identification of endogenous gibberellins in strawberry, including the novel gibberellins ga123, ga124 and ga125. | extracts of carboxylic acids from immature fruits of strawberry (fragaria x ananassa duch. cv. elsanta) were analysed for gibberellins by combined gas chromatography-mass spectrometry. the following previously characterised gibberellins were identified by comparison of their mass spectra and kovats retention indices (kris) with those of standards or published data: ga1, ga3, ga5, ga8, ga12, ga17, ga19, ga20, ga29, ga44, ga48, ga49, ga53, ga77, ga97, ga111 and ga112. evidence for endogenous 1-epi ... | 2000 | 11140520 |
| characterization of two divergent endo-beta-1,4-glucanase cdna clones highly expressed in the nonclimacteric strawberry fruit. | two cdnas clones (cel1 and cel2) encoding divergent endo-beta-1, 4-glucanases (egases) have been isolated from a cdna library obtained from ripe strawberry (fragaria x ananassa duch) fruit. the analysis of the amino acid sequence suggests that cel1 and cel2 egases have different secondary and tertiary structures and that they differ in the presence of potential n-glycosylation sites. by in vitro translation we show that cel1 and cel2 bear a functional signal peptide, the cleavage of which yields ... | 1999 | 10198101 |
| isolation of genomic dna containing a cytosolic ascorbate peroxidase gene (apxsc) from the strawberry (fragaria x ananassa). | we isolated a genomic dna harboring a cytosolic ascorbate peroxidase gene (apxsc) from a genomic library of the strawberry (fragaria x ananassa). restriction mapping and sequence analyses showed that the dna is composed of 2.5 kb of the full-length apxsc gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. the apxsc genomic dna contains 10 exons and 9 introns, which is similar to the structure of pea apxi. a primer extension analysis suggested that the transcription of ... | 1998 | 9720218 |
| 2,5-dimethyl-4-hydroxy-3[2h]-furanone 6'o-malonyl-beta-d-glucopyranoside in strawberry fruits. | 2,5-dimethyl-4-hydroxy-3[2h]-furanone 6'-o-malonyl-beta-d-glucopyranoside was isolated from a glycosidic extract of strawberry fruit (fragaria x ananassa, cv. senga sengana) by means of countercurrent chromatography and reverse-phase hplc. identification was achieved by comparison of chromatographic and 1h, 13c and 2d-nmr, as well as mass spectral data with those of the synthesized reference compound. in ripe strawberry fruit, a 1:1 ratio of the malonylated glucoside to its deacylated glucoconju ... | 1996 | 8987510 |
| detection and isolation of nepoviruses on strawberry in the czech republic. | arabis mosaic, strawberry latent ringspot, tomato black ring and raspberry ringspot nepoviruses were monitored using double sandwich enzyme-linked immunosorbent assay (das-elisa) in 18 cultivars of strawberry fragaria x ananassa duch. in the czech republic. arabis mosaic and strawberry latent ringspot viruses were detected, isolated and characterized on differential host plants and by electron microscopy. both viruses were purified and antisera to them were prepared. | 1995 | 8578992 |
| a fruit-specific putative dihydroflavonol 4-reductase gene is differentially expressed in strawberry during the ripening process. | a cdna clone encoding a putative dihydroflavonol 4-reductase gene has been isolated from a strawberry (fragaria x ananassa cv chandler) dna subtractive library. northern analysis showed that the corresponding gene is predominantly expressed in fruit, where it is first detected during elongation (green stages) and then declines and sharply increases when the initial fruit ripening events occur, at the time of initiation of anthocyanin accumulation. the transcript can be induced in unripe green fr ... | 1998 | 9625725 |
| cloning and characterization of cdnas from genes differentially expressed during the strawberry fruit ripening process by a mast-pcr-sbds method. | a vast number of clones carrying cdnas from genes differentially expressed along the strawberry (fragaria x ananassa c.v. chandler) fruit ripening process has been isolated by screening of a subtractive cdna library. the library was constructed and screened using a powerful procedure that combines the differential screening technique with a southern blot screening by means of the polymerase chain reaction (pcr-sbds procedure). several clones have been partially sequenced and characterized and ma ... | 1997 | 9177756 |
| haploid plant regeneration from anther cultures of three north american cultivars of strawberry (fragaria x ananassa duch.). | a study was conducted to maximize plant regeneration frequencies from cultured anthers of 'chandler', 'honeoye', and 'redchief' strawberries (fragaria x ananassa duch.). a comparison of auxins (iaa, naa), cytokinins (ba, bpa, kin) and carbohydrates (sucrose, glucose, maltose) in ms medium showed that the highest shoot regeneration across cultivars (8%) occurred when using a medium containing 2 mg/l iaa, 1 mg/l ba, and 0.2 m glucose. a comparison of ms, nn, and h1 inorganic medium (a new formulat ... | 1996 | 24178272 |
| sequence analysis of 18s ribosomal rna gene in fragaria x ananassa duch. cultivated octoploid strawberry. | | 1992 | 1600161 |
| overwintering hosts for the exotic leafroller parasitoid, colpoclypeus florus: implications for habitat manipulation to augment biological control of leafrollers in pome fruits. | thirty sites of managed and native habitats were surveyed for leafrollers (lepidoptera: tortricidae) in the apple producing region of central washington state and northern oregon from september through november 1997-2000 to discover species that supported overwintering by the parasitoid colpoclypeus florus (walker) (hymenoptera: eulophidae). c. florus, a species introduced from europe, requires medium to large host larvae late in autumn on which to overwinter, and few leafroller species display ... | 2010 | 20673193 |
| the inheritance of vegetative growth traits in strawberries (fragaria x ananassa) grown at low temperatures and their relationship to field productivity. | the genetic relationship between vegetative growth at low temperatures and productivity was investigated for strawberries grown in controlled and field environments. genotypes from 20 biparental crosses were grown in controlled environments with 11°, 14°, and 17 °c days, 11 °c nights, and 11-h daylength to simulate a range of winter growing conditions expected in mediterranean environments. individual plants were scored for two initial runner traits and eight vegetative growth traits. significan ... | 1993 | 24190209 |
| genetic correlations between vegetative growth traits and productivity at different within-season intervals for strawberries (fragaria x ananassa). | genetic and environmental relationships between vegetative growth and production traits at different intervals within a single season were investigated using unselected strawberry genotypes from 20 biparental crosses and their parents. vegetative growth and productivity patterns differed between test locations and larger yields were detected where fall growth was greatest. positive genetic correlations were detected between fall growth increments and mid-season production traits, but fall growth ... | 1993 | 24196151 |
| transient gene expression in strawberry (fragaria x ananassa duch.) protoplasts and the recovery of transgenic plants. | a transient ß-glucuronidase (gus)-assay was performed to evaluate electroporation parameters and optimize dna delivery conditions into strawberry protoplasts. optimal gus-activity was obtained when protoplasts were subjected to 400 v/cm for 20 ms. gus-activity could be further increased by the addition of carrier dna to the electroporation mixture. callus selected on 10 μg/ml hygromycin produced shoots which exhibited gus-activity. the transformed nature of the shoots obtained after selection wa ... | 1992 | 24213496 |
| isolation of nucleic acids from plants by differential solvent precipitation. | the purification of nucleic acids from plant tissue is often made difficult by the presence of contaminating carbohydrate polymers and polyphenols. a procedure for the simultaneous isolation of dna and translatable rna from plants is described. the method removes most of the polysaccharides and polyphenols extracted with nucleic acids in a single step by taking advantage of differences in solubility of these compounds in the solvent 2-butoxyethanol. stepwise addition of 2-butoxyethanol to phenol ... | 1991 | 1716071 |
| photocontrol of petiole elongation in light-grown strawberry plants. | the mode of phytochrome control of elongation growth was studied in fully-green strawberry (fragaria x ananassa duch.) plants. petiole growth showed two distinct types of response to light. in one, the end-of-day response, petioles were lengthened by low-intensity far-red irradiation for 1 h immediately following the 8 h photoperiod. the response was little or no greater with prolonged exposure and less when the start of far-red was delayed. it was already evident in the first leaf to emerge aft ... | 1976 | 24424755 |
| strawberry fruit rot caused by neopestalotiopsis iranensis sp. nov., and n. mesopotamica. | a new species of neopestalotiopsis based on both morphological and molecular characteristics is described. neopestalotiopsis iranensis sp. nov. isolated from rotted strawberry (fragaria ananassa) fruits as well as from stolon and leaf lesions in kurdistan province, iran. initially, light tan and sunken spots developed on fruits and resulted in a soft decay of the fruit flesh. the new species is morphologically distinguished from similar species with different conidium size and by possessing long ... | 2016 | 26659835 |
| feronia/fer-like receptor kinases integrate and modulate multiple signaling pathways in fruit development and ripening. | ripening of fleshy fruits is a complex process that involves dramatic changes in color, texture, flavor, and aroma, which is essentially regulated by multiple hormone signals. although the metabolic mechanisms for the regulation of fruit development and ripening have been studied extensively, little is known about the signaling mechanisms underlying this process. feronia has been increasingly suggested to be implicated in multiple signaling pathways. in a recent publication, we showed that a fer ... | 2017 | 29215944 |
| scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. | the antioxidant activities against superoxide radicals (o(2)(*)(-)), hydrogen peroxide (h(2)o(2)), hydroxyl radicals (oh(*)), and singlet oxygen ('o(2)) was evaluated in fruit juice from different cultivars of thornless blackberries (rubus sp.), blueberries (vaccinium spp.), cranberries (vaccinium macrocarpon aiton), raspberries (rubus idaeus l. and rubus occidentalis l.), and strawberries (fragaria x ananassa duch.). among the different cultivars, juice of 'hull thornless' blackberry, 'earliglo ... | 2000 | 11087538 |
| the influence of photoperiodic growth condition on isolation of rna from strawberry (fragaria x ananassa duch.) tissue. | purification of high-quality rna from different strawberry tissues is often affected by the presence of high levels of contamination by polysaccharides and phenolic compounds. with the protocol detailed here we describe for the first time total rna purification from petiole tissue. treating the plants used as source of material with short-daylight regime prior the extraction we are able to obtain rna suitable for further applications such as in vitro translation, rt-pcr, and rna blot analysis. t ... | 2000 | 10986699 |
| the effect of high hydrostatic pressure on the strawberry anthocyanins. | color stability of fruit juice made from strawberries (fragaria x ananassa, cv. elsanta) that were subjected to high hydrostatic pressure was studied by measuring the anthocyanin content. high hysrostatic pressure is a method of preservation of food alternative to heat treatment. it is therefore essential to assess the impact of high pressure on color molecules. samples were pressurized under 200, 400, 600, and 800 mpa for 15 min at a temperature controlled between 18 and 22 degrees c. after app ... | 2000 | 10898617 |
| sequence analysis of cultivated strawberry (fragaria × ananassa duch.) using microdissected single somatic chromosomes. | cultivated strawberry (fragaria × ananassa duch.) has homoeologous chromosomes because of allo-octoploidy. for example, two homoeologous chromosomes that belong to different sub-genome of allopolyploids have similar base sequences. thus, when conducting de novo assembly of dna sequences, it is difficult to determine whether these sequences are derived from the same chromosome. to avoid the difficulties associated with homoeologous chromosomes and demonstrate the possibility of sequencing allopol ... | 2017 | 29118823 |
| volatile organic compounds from wickerhamomyces anomalus, metschnikowia pulcherrima and saccharomyces cerevisiae inhibit growth of decay causing fungi and control postharvest diseases of strawberries. | the effectiveness of wickerhamomyces anomalus, metschnikowia pulcherrima and saccharomyces cerevisiae as biocontrol agents on postharvest decay of strawberry (fragaria x ananassa, cv. 'alba') fruit, and their inhibitory activities on some decay-causing fungi were evaluated. volatile organic compounds from these yeasts decreased mycelial growth of botrytis cinerea by 69%, and by less for monilinia fructicola, alternaria alternata, aspergillus carbonarius, penicillium digitatum, cladosporium spp., ... | 2018 | 29107842 |
| nucleotide sequence of an alcohol dehydrogenase gene in octoploid strawberry (fragaria x ananassa duch). | | 1990 | 2102862 |
| soft mechanical stimulation induces a defense response against botrytis cinerea in strawberry. | genes associated with plant mechanical stimulation were found in strawberry genome. a soft mechanical stimulation (sms) induces molecular and biochemical changes in strawberry plants, conferring protection against botrytis cinerea. plants have the capacity to induce a defense response after exposure to abiotic stresses acquiring resistance towards pathogens. it was reported that when leaves of arabidopsis thaliana were wounded or treated with a soft mechanical stimulation (sms), they could resis ... | 2018 | 29032427 |
| role of ethylene and related gene expression in the interaction between strawberry plants and the plant growth-promoting bacterium azospirillum brasilense. | • the induced systemic resistance (isr) is one of the indirect mechanisms of growth promotion exerted by plant growth-promoting bacteria, and can be mediated by ethylene (et). we assessed the et production and the expression of related genes in the azospirillum-strawberry plant interaction. • et production was evaluated by gas chromatography in plants inoculated or not with a. brasilense rec3. also, plants were treated with agno3 , an inhibitor of et biosynthesis; with 1-aminocyclopropane-1-carb ... | 2018 | 29350442 |
| the potassium channel fatpk1 plays a critical role in fruit quality formation in strawberry (fragaria × ananassa). | potassium (k(+) ), an abundant cation in plant cells, is important in fruit development and plant resistance. however, how cellular k(+) is directed by potassium channels in fruit development and quality formation of strawberry (fragaria × ananassa) is not yet fully clear. here, a two-pore k(+) (tpk) channel gene in strawberry, fatpk1, was cloned using reverse transcription-pcr. a green fluorescent protein subcellular localization analysis showed that fatpk1 localized in the vacuole membrane. a ... | 2017 | 28851008 |
| pedigree-based analysis in a multiparental population of octoploid strawberry reveals qtl alleles conferring resistance to phytophthora cactorum. | understanding the genetic architecture of traits in breeding programs can be critical for making genetic progress. important factors include the number of loci controlling a trait, allele frequencies at those loci, and allele effects in breeding germplasm. to this end, multiparental populations offer many advantages for quantitative trait locus (qtl) analyses compared to biparental populations. these include increased power for qtl detection, the ability to sample a larger number of segregating ... | 2017 | 28592652 |
| ultraviolet light-c increases antioxidant capacity of the strawberry (fragaria x ananassa) in vitro and in high-fat diet-induced obese rats. | flavonoids and polyphenols from the strawberry and other fruits have been proposed to reduce the oxidative stress produced by the obesity and her complications. moreover, it has been proposed that irradiation with uv-c to strawberry may increase the antioxidant capacity of this fruit. the aim of the present study was to explore the effects of the uv-c on antioxidant capacity of strawberry in vitro and in vivo. strawberry slices were irradiated with ultraviolet light-c (uv-c) at 1.2 w/m2/16.5 min ... | 2017 | 28948018 |
| anthocyanin management in fruits by fertilization. | anthocyanins are water-soluble vacuolar plant pigments that are mainly synthesized in epidermal layers and the flesh of fruits such as apples, cherries, grapes, and other berries. because of their attractive red to purple coloration and their health-promoting potential, anthocyanins are significant determinants for the quality and market value of fruits and fruit-derived products. in crops, anthocyanin accumulation in leaves can be caused by nutrient deficiency which is usually ascribed to insuf ... | 2018 | 29297687 |
| a leu-rich repeat receptor-like protein kinase, faripk1, interacts with aba receptor, faabar, to regulate strawberry (fragaria × ananassa) fruit ripening. | strawberry (fragaria × ananassa) is a model plant for studying non-climacteric fruit ripening regulated by abscisic acid (aba), however, its defined molecular mechanisms are yet not fully understood. here, a strawberry predicted leu-rich repeat (lrr) receptor-like kinase, red-initial protein kinase 1 (faripk1), was screened and shown to interact with a putative aba receptor, faabar, using a yeast two-hybrid assay. this association was confirmed by bimolecular fluorescence complementation and co- ... | 2017 | 29281111 |
| molecular mechanism of action of pelargonidin-3-o-glucoside, the main anthocyanin responsible for the anti-inflammatory effect of strawberry fruits. | fragaria x ananassa duch., popularly called strawberry, is known for its worldwide consumption and important biological activities, and these effects are related to its high concentration of anthocyanins. pelargonidin-3-o-glucoside (p3g) is a major anthocyanin found in strawberry, and was evaluated for its anti-inflammatory action in experimental models. the effect of strawberry extract and p3g, on leukocyte migration, exudation levels and many inflammatory mediators, was therefore evaluated in ... | 2018 | 29277228 |
| effects of phenolic compounds on growth of colletotrichum spp. in vitro. | colletotrichum acutatum is responsible for anthracnose fruit rot, one of the most devastating diseases in strawberry. phenolic compounds have been described as contributors to anthracnose resistance in strawberry (fragaria x ananassa, duch.). six isolates of colletotrichum acutatum and four isolates of three other colletotrichum species, c. gloeosporioides, c. fragariae, and c. graminicola, associated with disease symptoms were investigated in this study. the potential inhibitory effect of pheno ... | 2017 | 29247336 |
| arabinogalactan proteins: immunolocalization in the developing ovary of a facultative apomict fragaria x ananassa (duch.). | arabinogalactan proteins are present in the extracellular matrix and their occurrence is developmentally regulated. the studies were carried out to localise arabinogalactan proteins in ovary cells of fragaria x ananassa duchesne (strawberry) during megasporogenesis, megagametogenesis, and formation of the embryo. | 2017 | 29223065 |
| optimisation of trans-cinnamic acid and hydrocinnamyl alcohol production with recombinant saccharomyces cerevisiae and identification of cinnamyl methyl ketone as a by-product. | trans-cinnamic acid (tca) and hydrocinnamyl alcohol (hcinoh) are valuable aromatic compounds with applications in the flavour, fragrance and cosmetic industry. they can be produced with recombinant yeasts from sugars via phenylalanine after expression of a phenylalanine ammonia lyase (pal) and an aryl carboxylic acid reductase. here, we show that in saccharomyces cerevisiae a pal enzyme from the bacterium photorhabdus luminescens was superior to a previously used plant pal enzyme for the product ... | 2017 | 29186481 |
| analysis of major paralogs encoding the fra a 1 allergen based on their organ-specificity in fragaria × ananassa. | fra a 1 protein in strawberry causes oral allergic syndrome. over 39 fra a 1 paralogs have been identified in strawberry genome. fra a 1.01 is major accumulating protein in edible organs. strawberry fruits contain allergenic proteins that cause oral allergic syndrome. the hypothesized major allergen is fra a 1, an ortholog of the birch pollen allergen protein bet v 1. we organized fra a 1 genes and analyzed their localizations at the transcriptional and translational levels. in total, 15 new fra ... | 2017 | 29177844 |
| selenium biofortification in fragaria × ananassa: implications on strawberry fruits quality, content of bioactive health beneficial compounds and metabolomic profile. | selenium (se) is an essential nutrient for humans, due to its antioxidant properties, whereas, to date, its essentiality to plants still remains to be demonstrated. nevertheless, if added to the cultivation substrate, plants growth resulted enhanced. however, the concentration of se in agricultural soils is very variable, ranging from 0.01 mg kg-1 up to 10 mg kg-1 in seleniferous areas. therefore several studies have been performed aimed at bio-fortifying crops with se and the approaches exploit ... | 2017 | 29163609 |
| a new perspective on polyploid fragaria (strawberry) genome composition based on large-scale, multi-locus phylogenetic analysis. | the subgenomic compositions of the octoploid (2n = 8× = 56) strawberry (fragaria) species, including the economically important cultivated species fragaria x ananassa, have been a topic of long-standing interest. phylogenomic approaches utilizing next-generation sequencing technologies offer a new window into species relationships and the subgenomic compositions of polyploids. we have conducted a large-scale phylogenetic analysis of fragaria (strawberry) species using the fluidigm access array s ... | 2017 | 29045639 |
| the complete chloroplast genome sequence of strawberry (fragaria × ananassa duch.) and comparison with related species of rosaceae. | compared with other members of the family rosaceae, the chloroplast genomes of fragaria species exhibit low variation, and this situation has limited phylogenetic analyses; thus, complete chloroplast genome sequencing of fragaria species is needed. in this study, we sequenced the complete chloroplast genome of f. × ananassa 'benihoppe' using the illumina hiseq 2500-pe150 platform and then performed a combination of de novo assembly and reference-guided mapping of contigs to generate complete chl ... | 2017 | 29038765 |
| the fruit-specific transcription factor fadof2 regulates the production of eugenol in ripe fruit receptacles. | only a few transcription factors have been described in the regulation of the strawberry (fragaria x ananassa) fruit ripening process. using a transcriptomic approach, we identified and functionally characterized fadof2, a dof-type ripening-related transcription factor, which is hormonally regulated and specific to the receptacle, though high expression levels were also found in petals. the expression pattern of fadof2 correlated with eugenol content, a phenylpropanoid volatile, in both fruit re ... | 2017 | 28981772 |
| spatial and temporal localization of flavonoid metabolites in strawberry fruit (fragaria × ananassa). | flavonoids are important metabolites in strawberries (fragaria × ananassa) because they accomplish an extensive collection of physiological functions and are valuable for human health. however, their localization within the fruit tissue has not been extensively explored. matrix-assisted laser desorption/ionization mass spectrometric imaging (maldi-msi) was employed to shed light on the spatial distribution of flavonoids during fruit development. one wild-type (wt) and two transgenic lines were c ... | 0 | 28409937 |
| plant gnotobiology: epiphytic microbes and sustainable agriculture. | in 1963, a monograph by thomas d. luckey entitled germfree life and gnotobiology was published, with a focus on animals treated with microbes and reference to the work of louis pasteur (1822-1895). here, we review the history and current status of plant gnotobiology, which can be traced back to the experiments of jean-baptiste boussingault (1801-1887) published in 1838. since the outer surfaces of typical land plants are much larger than their internal areas, embryophytes "wear their guts on the ... | 2016 | 27830978 |
| effects of nano-tio2 -ldpe packaging on postharvest quality and antioxidant capacity of strawberry (fragaria ananassa duch.) stored at refrigeration temperature. | nano-tio2 -low-density polyethylene (ntldpe) packaging was prepared, and the effects of ntldpe packaging on quality and antioxidant capacity of strawberry fruits were investigated. | 0 | 27282949 |
| microbial synthesis of a branched-chain ester platform from organic waste carboxylates. | processing of lignocellulosic biomass or organic wastes produces a plethora of chemicals such as short, linear carboxylic acids, known as carboxylates, derived from anaerobic digestion. while these carboxylates have low values and are inhibitory to microbes during fermentation, they can be biologically upgraded to high-value products. in this study, we expanded our general framework for biological upgrading of carboxylates to branched-chain esters by using three highly active alcohol acyltransfe ... | 2016 | 29142826 |
| iodide and iodate effects on the growth and fruit quality of strawberry. | iodine deficiency is an environmental health problem affecting one-third of the global population. an iodine biofortification hydroponic experiment was conducted to explore the iodide and iodate uptake characteristics of strawberry plants, to measure the dosage effects of iodine on plant growth and to evaluate the influence of i- or io3- application on fruit quality. | 2017 | 26992053 |
| genotyping-by-sequencing enables linkage mapping in three octoploid cultivated strawberry families. | genotyping-by-sequencing (gbs) was used to survey genome-wide single-nucleotide polymorphisms (snps) in three biparental strawberry (fragaria × ananassa) populations with the goal of evaluating this technique in a species with a complex octoploid genome. gbs sequence data were aligned to the f. vesca 'fvb' reference genome in order to call snps. numbers of polymorphic snps per population ranged from 1,163 to 3,190. linkage maps consisting of 30-65 linkage groups were produced from the snp sets d ... | 2017 | 28875078 |
| data in support of comparative analysis of strawberry proteome in response to controlled atmosphere and low temperature storage using a label-free quantification. | to elucidate the mechanisms contributing to fruit responses to senescence and stressful environmental stimuli under low temperature (lt) and controlled atmosphere (ca) storage, a label-free quantitative proteomic investigation was conducted in strawberry (fragaria ananassa, duch. cv. 'akihime'). postharvest volatile compounds were characterized following storage under different conditions. the observed post-storage protein expression profiles may be associated with delayed senescence features in ... | 2015 | 26217742 |
| edible active coatings based on pectin, pullulan, and chitosan increase quality and shelf life of strawberries (fragaria ananassa). | edible active coatings (eacs) based on pectin, pullulan, and chitosan incorporated with sodium benzoate and potassium sorbate were employed to improve the quality and shelf life of strawberries. fruits were washed, disinfected, coated by dipping, packed, and stored at 4 °c for 15 d. application of eacs reduced (p < 0.05) weight loss and fruit softening and delayed alteration of color (redness) and total soluble solids content. in contrast, ph and titratable acidity were not affected (p > 0.05) t ... | 2015 | 26189365 |
| procedures of mitochondria purification and gene expression to study alternative respiratory and uncoupling pathways in fruits. | we describe detailed procedures to get intact and well-coupled mitochondria from a variety of fruit species such as papaya (carica papaya), guava (psidium guajava), tomato (solanum lycopersicum), and strawberry (fragaria x ananassa) as well as the protocols to assess the capacities of aox and ucp pathways in intact fruit mitochondria. the procedures presented here were tested for the species mentioned above; their use with other types of fruits must be tested for yield of intact and active mitoc ... | 2017 | 28871541 |
| label-free quantitative proteomics to investigate strawberry fruit proteome changes under controlled atmosphere and low temperature storage. | to elucidate the mechanisms contributing to fruit responses to senescence and stressful environmental stimuli under low temperature (lt) and controlled atmosphere (ca) storage, a label-free quantitative proteomic investigation was conducted in strawberry (fragaria ananassa, duch. cv. 'akihime'). postharvest physiological quality traits including firmness, total soluble solids, total acidity, ascorbic acid and volatile production were characterized following storage under different conditions. th ... | 2015 | 25753123 |