| a rapeseed cold-inducible transcript encodes a phosphoenolpyruvate carboxykinase. | we have isolated a clone corresponding to a new cold-regulated gene from a cdna library made from rapeseed (brassica napus cv samourai) cold-acclimated etiolated seedlings. sequence analysis and homology searches showed that this clone encodes a protein highly homologous to the atp-dependent phosphoenolpyruvate carboxykinase (pepck; ec 4.1.1.49) from saccharomyces cerevisiae, trypanosoma, rhizobium sp., and escherichia coli; we refer to the b. napus clone as bnpepck. a potential atp-binding site ... | 1995 | 7480349 |
| pollen allergen homologues in barley and other crop species. | pollen from 10 agricultural plant species was surveyed for the presence of proteins crossreactive with group i, group iv and group ix allergens. barley (hordeum vulgare), maize (zea mays), rye (secale cerale), triticale (xtriticosecale cereale), oats (avena sativa), canola (brassica napus) and sunflower (helianthus annus) pollens contained numerous allergen cognate proteins. northern blot analysis of barley pollen rna revealed the presence of group i and group ix allergen transcripts. the barley ... | 1995 | 7537171 |
| phenotypic alterations of petal and sepal by ectopic expression of a rice mads box gene in tobacco. | floral organ development is controlled by a group of regulatory factors containing the mads domain. in this study, we have isolated and characterized a cdna clone from rice, osmads3, which encodes a mads-domain containing protein. the osmads3 amino acid sequence shows over 60% identity to ag of arabidopsis, ple of antirrhinum majus, and ag/ple homologues of petunia, tobacco, tomato, brassica napus, and maize. homology in the mads box region is most conserved. rna blot analysis indicated that the ... | 1995 | 7579155 |
| a competitive polymerase chain reaction to quantify dna of leptosphaeria maculans during blackleg development in oilseed rape. | an assay based on the competitive polymerase chain reaction technique was developed to quantify leptosphaeria maculans during blackleg disease development in oilseed rape leaves. by means of primers specific to the highly virulent type of l. maculans, a heterologous internal control template was prepared by amplifying and cloning dna from leptosphaeria korrae under low-stringency annealing conditions. coamplification of l. maculans with the internal control dna provided accurate quantification o ... | 1995 | 7579620 |
| agrobacterium-mediated transformation of protoplasts from oilseed rape (brassica napus l.). | | 1995 | 7581685 |
| agrobacterium-mediated transformation of stem disks from oilseed rape (brassica napus l.). | | 1995 | 7581686 |
| trans mono- and polyunsaturated fatty acids in human milk. | the aim of the present study was to identify the trans isomers of c18 fatty acids in some human milk samples. | 1995 | 7588503 |
| rapid purification of malate synthase from cotyledons of brassica napus l. | a rapid and efficient method for the purification of malate synthase, an enzyme uniquely confined to glyoxysomes, from cotyledons of brassica napus l. has been developed. the two step purification procedure is based on the consequent utilization of the tendency of malate synthase to form high molecular weight aggregates. malate synthase was purified 75-fold to apparent homogeneity with a specific activity of 180 nkat/mg protein. the estimated molecular weight of malate synthase subunits was 63 k ... | 1995 | 7589540 |
| molecular cloning and sequence of the cytoplasmic ribosomal protein s15a gene from agaricus bisporus. | we have isolated an agaricus bisporus cdna which encodes an open reading frame of 130 amino acids. a comparison with the genbank database shows that the deduced amino acid sequence of this open reading frame is highly homologous to the small subunit ribosomal proteins s15a of brassica napus and drosophila melanogaster and to the small subunit ribosomal proteins s24 of strongylocentrotus purpuratus and saccharomyces cerevisiae. | 1995 | 7614376 |
| dietary lipid and iron status modulate lipid peroxidation in rats with induced adjuvant arthritis. | we studied the effect of dietary lipids on iron metabolism and lipid peroxidation during induced adjuvant arthritis and/or iron overload in rats. we compared a control diet containing corn oil and rapeseed oil with a diet devoid of polyunsaturated fatty acids containing only tripalmitin as lipids. four subgroups of rats were used with each diet: without further treatment, with induction of adjuvant arthritis, with iron overload, and with induction of adjuvant arthritis and iron overload. the pro ... | 1995 | 7616310 |
| horizontal transfer in the phytopathogenic fungal genus leptosphaeria and host-range expansion. | all isolates examined of the phytopathogenic fungus leptosphaeria maculans that are aggressive to brassica napus and brassica rapa have a repetitive element, lmr1. horizontal transfer of lmr1 to an isolate of a closely related species of leptosphaeria correlates with the expansion of the host range of this isolate to include brassica juncea. | 1995 | 7627459 |
| organization, inheritance and expression of acetohydroxyacid synthase genes in the cotton allotetraploid gossypium hirsutum. | the acetohydroxyacid synthase (ahas) gene family of the cotton ad allotetraploid gossypium hirsutum has been cloned and characterized. we have identified six different ahas genes from an analysis of genomic clones and southern blots of genomic dna. four of the six genes are organized as tandem pairs, in which the genes are separated by only 2-3 kb. conservation of restriction fragment length polymorphisms between g. hirsutum and a-genome and d-genome-containing diploid cottons was sufficient to ... | 1995 | 7640356 |
| functional dissection of the promoter of the pollen-specific gene ntp303 reveals a novel pollen-specific, and conserved cis-regulatory element. | regulatory elements within the promoter of the pollen-specific ntp303 gene from tobacco were analysed by transient and stable expression analyses. analysis of precisely targeted mutations showed that the ntp303 promoter is not regulated by any of the previously described pollen-specific cis-regulatory elements. however, two adjacent regions from -103 to -86 bp and from -86 to -59 bp were shown to contain sequences which positively regulated the ntp303 promoter. both of these regions were capable ... | 1995 | 7655507 |
| identification of 1,3-butadiene, benzene, and other volatile organics from wok oil emissions. | as part of a program to determine the underlying factors responsible for genotoxicity and perhaps lung cancer risk in chinese women, we qualitatively identified the volatile components emitted during the heating of cooking oils to 265 degrees c. 1,3-butadiene, benzene, and a series of aldehydes, olefins, and saturated hydrocarbons were elucidated in vapors from chinese rapeseed oil. on a relative basis, the intensity of 1,3-butadiene vapors from this were 15.7-, 6.3-, and 1.4-fold greater than i ... | 1995 | 7663151 |
| [glycerol as a feed component in fattening pigs]. | the production of motor fuel from rapeseed oil forms large amounts of glycerol as a waste product (about 100 g per kg oil). the aim of this experiment was to test glycerol as a component in diets of fattening pigs. in two experiments received 48 pigs (pietrain x f1, landrace x german breed) up to 30% glycerol in barley-soya bean oil meal diets. six pigs per group with an initial weight of 32 kg (experiment 1) and 31.2 kg (experiment 2) in average received the diets semi ad libitum during the who ... | 1995 | 7668991 |
| duodenal infusion of rapeseed oil in midlactation cows. 6. interaction with niacin on dairy performance and nutritional balance. | rapeseed oil and niacin, alone or in combination, were infused continuously into the proximal duodenum of ruminally and duodenally fistulated midlactation cows in a 4 x 4 latin square design. although niacin in plasma was higher for cows infused with niacin no significant effect occurred on milk production and composition. when cows were infused with oil, milk production and fat content were unchanged, milk protein content was decreased, and lactose content was increased. the magnitude of decrea ... | 1995 | 7673524 |
| conservative amino acid substitutions of the c-terminal tripeptide (ala-arg-met) on cottonseed isocitrate lyase preserve import in vivo into mammalian cell peroxisomes. | the purpose of this research was twofold, a) to directly demonstrate import in vivo of a native plant peroxisomal protein into peroxisomes of transiently transfected mammalian cells, and b) to identify the targeting signal and amino acid substitutions thereof which preserve translocation of this plant protein into these peroxisomes. the protein selected for study was cottonseed isocitrate lyase (icl), a glyoxylate cycle enzyme which participates in storage oil mobilization in oilseed cotyledons. ... | 1994 | 7720722 |
| plasma thyroxine concentration in non-pregnant and lactating mink, and effect of dietary rapeseed oil in the reproduction period. | effect of dietary rapeseed oil from 00-varieties of rapeseed (0, 1.5% or 3% respectively in the wet compounded diets) on plasma thyroxine (t4), reproductive performance and kit weight gain during lactation was investigated with 3 groups of each 20 mink females. plasma t4, which has not previously been reported for female mink, was significantly lower in lactating than in non-pregnant females. unlike in an earlier experiment with growing male mink, it was not affected by dietary rapeseed oil. rep ... | 1994 | 7733807 |
| is dietary erucic acid hepatotoxic in pregnancy? an experimental study in rats and hamsters. | the hypothesis that dietary erucic acid may contribute to the pathogenesis of intrahepatic cholestasis of pregnancy has been examined in pregnant rats and hamsters after prolonged feeding of diets containing 25% rapeseed oil rich in erucic acid (40% of fatty acids) or corn oil, without erucic acid. both dietary oils were well tolerated, although weight gain was 17% to 20% less in animals receiving rapeseed oil. rats and hamsters were studied on the last day of pregnancy and compared with age- an ... | 1995 | 7737644 |
| foreign dna sequences are received by a wild-type strain of aspergillus niger after co-culture with transgenic higher plants. | different transgenic plants of brassica napus, brassica nigra, datura innoxia and vicia narbonensis expressing the hph gene under the control of the 35s promoter were co-cultivated with mycelial material of aspergillus niger in microcosms under sterile conditions. a significantly higher number of hygromycin b-resistant colonies of re-isolated fungi was obtained if compared with co-cultures with non-transgenic plants. the hph gene and other foreign sequences could be detected in some of the resis ... | 1994 | 7750149 |
| decrease of 12-hydroxyeicosatetraenoic acid production in mouse lungs following dietary oleic anilide consumption: implications for the toxic oil syndrome. | a study was performed to examine the ability of dietary oleic anilide to alter 12-hydroxyeicosatetraenoic acid (12-hete) production. the structure of oleic anilide, synthesized by reacting oleic acid with aniline, was confirmed by mass spectrometry. the purity of oleic anilide, 75%, was measured by gas chromatography. oleic acid, which constituted the remaining 25%, is a major component of the rapeseed oil vehicle. balb/c mice were fed oleic anilide as 0.75% of their diet by weight for three wee ... | 1995 | 7755405 |
| further characterization of auxin-regulated mrnas in hypocotyl sections of mung bean [vigna radiata (l.) wilczek]: sequence homology to genes for fatty-acid desaturases and atypical late-embryogenesis-abundant protein, and the mode of expression of the mrnas. | the amino-acid sequence deduced from the nucleotide sequence of an auxin-regulated gene, arg1, isolated from auxin-treated sections of mung-bean hypocotyls (yamamoto et al. 1992, plant cell physiol. 33, 13-20), is 69% identical to that of a delta 15 fatty-acid desaturase from brassica napus l. that is localized in the endoplasmic reticulum. the arg1 message is present at high levels in the hook portion of the hypocotyl, the plumule and the root tip of 3-d-old etiolated seedlings. the amino-acid ... | 1994 | 7764402 |
| simultaneous induction of postabscission and germination mrnas in cultured dicotyledonous embryos. | cloned mrnas identify three programs of gene expression in cotton (gossypium hirsutum l.) embryos that are associated with the maturation (reserve accumulation) stage, the postabscission stage, which is marked by expression of late-embryogenesis-abundant (lea) mrnas, and germination (broadly defined as including all events through early postgerminative growth). in order to test if the regulation of these programs is the same in other dicotyledonous species, their expression was studied in normal ... | 1994 | 7764404 |
| oleosins in the gametophytes of pinus and brassica and their phylogenetic relationship with those in the sporophytes of various species. | oleosins, which are structural proteins on the surface of intracellular oil bodies, have been found in the sporophytic seeds of angiosperms. here, we report an oleosin from the female gametophyte of gymnosperm pinus ponderosa laws. seed and another oleosin from the male gametophyte of brassica napus l. with the pine seed gametophyte, we identified two putative oleosins of 15 and 10 kda, which are similar to the oleosins in angiosperm seeds in terms of their presence in the oil bodies in massive ... | 1994 | 7764875 |
| modification of brassica napus seed oil by expression of the escherichia coli fabh gene, encoding 3-ketoacyl-acyl carrier protein synthase iii. | the escherichia coli fabh gene encoding 3-ketoacyl-acyl carrier protein synthase iii (kas iii) was isolated and the effect of overproduction of bacterial kas iii was compared in both e. coli and brassica napus. the change in fatty acid profile of e. coli was essentially the same as that reported by tsay et al. (j biol chem 267 (1992) 6807-6814), namely higher c14:0 and lower c18:1 levels. in our study, however, an arrest of cell growth was also observed. this and other evidence suggests that in ... | 1995 | 7766878 |
| petroselinic acid from dietary triacylglycerols reduces the concentration of arachidonic acid in tissue lipids of rats. | studies in vitro have revealed that triacylglycerols containing petroselinoyl [18:1(n-12)] moieties are hydrolyzed by pancreatic lipase at much lower rates than other triacylglycerols. to assess the lipolysis and absorption in vivo of such unusual triacylglycerols, diets containing 120 g seed oil triacylglycerols of coriander (coriandrum sativum) per kg diet at a level of 72 g 18:1(n-12) moieties/100 g oil were fed to a group of weaned male wistar rats without restriction for a period of 10 wk. ... | 1995 | 7782911 |
| mutagens from heated chinese and u.s. cooking oils. | the lung cancer incidence in chinese women is among the highest in the world, but tobacco smoking accounts for only a minority of the cancers. epidemiologic investigations of lung cancer among chinese women have implicated exposure to indoor air pollution from wok cooking, where the volatile emissions from unrefined cooking oils are mutagenic. | 1995 | 7791233 |
| characterisation of cdna and genomic clones encoding homologues of the 65 kda regulatory subunit of protein phosphatase 2a in arabidopsis thaliana. | two cdna species encoding sequences homologous to the 65 kda regulatory subunit (pr 65) of protein phosphatase 2a (pp2a) have been isolated from an arabidopsis thaliana cdna library. these were designated pdf1 and pdf2. pdf1 is 1795 bp long and by comparison with the human and porcine pp2a regulatory subunit sequences represents a full-length clone. it encodes a predicted polypeptide of 587 amino acid residues. pdf2 is truncated at the 5' end by 237 bp. the complete nucleotide sequences have bee ... | 1994 | 7811971 |
| efficacy of feeding glucosinolate-extracted crambe meal to broiler chicks. | glucosinolates and their breakdown products (nitriles) have long been implicated as toxic factors when feeding rapeseed (brassica napus) meals and crambe (crambe abyssinica) meals to poultry. accordingly, various methods have been developed to remove these compounds from the meals to enhance their value as feed supplements. glucosinolates and nitriles were extracted from commercially processed, defatted crambe meal by washing with water or various solvent-water mixtures: 50% isopropanol, 50% ace ... | 1994 | 7816729 |
| solubilization and characterization of diacylglycerol acyltransferase from microspore-derived cultures of oilseed rape. | particulate fractions prepared from microspore-derived (md) embryos of oilseed rape (brassica napus l. cv. reston) and an embryogenic md cell suspension culture of oilseed rape (b. napus l. cv. jet neuf) were used as a source of diacylglycerol acyltransferase (dgat, ec 2.3.1.20) for enzyme characterization and development of a solubilization procedure. dgat activity in the 1500-100,000 g fraction from md embryos was stimulated 4-5-fold by 3 to 4 mg of bsa/ml of reaction mixture. dgat activity fr ... | 1994 | 7818502 |
| decrease of brain phospholipid synthesis in free-moving n-3 fatty acid deficient rats. | the autoradiographic method with [14c]-docosahexaenoic acid ([14c]22:6 n-3) was used to determine whether a diet deficient in n-3 fatty acids, inducing a decrease in 22:6 n-3 circulating level, was associated with changes in local rates of phospholipid synthesis in the rat brain. as compared with rats fed a normal diet (peanut plus rapeseed oil), a n-3 fatty acid deficiency [peanut oil group (p group)] induced a generalized decrease (-35 to -76%) of 22:6 n-3 incorporation rates into phospholipid ... | 1995 | 7830085 |
| cloning and expression in escherichia coli of a novel thioesterase from arabidopsis thaliana specific for long-chain acyl-acyl carrier proteins. | an arabidopsis thaliana partial cdna was previously identified with a sequence similar to the lauroyl-acyl carrier protein (acp) thioesterase from umbellularia california (grellet et al., 1993, plant physiol. biochem. 31, 599-602). using this dna fragment, we isolated a 1.8-kb cdna coding for a 412-amino-acid preprotein. the deduced amino acid sequence is 51% identical to the lauroyl-acp thioesterase but only 39% identical to safflower oleoyl-acp thioesterase. the cdna was expressed in escherich ... | 1995 | 7840673 |
| processing of the 2s storage protein pronapin in brassica napus and in transformed tobacco. | the 2s protein napin in brassica napus is synthesized as a proprotein from which an n-terminal an an internal propeptide are removed. in order to investigate the mechanism of 2s storage-protein processing, n-terminal sequences were determined for the light and heavy chains of all major napin isoforms isolated. mutants of a napin gene, with deletions of different portions of the propeptides, were transformed into tobacco and napin protein was isolated. napin light and heavy chains were separated ... | 1995 | 7851402 |
| functional complementation of a yeast vesicular transport mutation ypt1-1 by a brassica napus cdna clone encoding a small gtp-binding protein. | a cdna clone (bra) encoding a small gtp-binding protein was isolated from brassica napus by screening a root cdna library with a degenerate oligonucleotide probe that corresponds to a highly conserved gtp-binding domain of the ras superfamily. sequence analysis shows that the clone contains an open reading frame of 219 amino acid residues with the estimated molecular mass of 24379 da and this coding region contains all the conserved motifs of the ras superfamily. the deduced amino acid sequence ... | 1994 | 7858213 |
| classification and expression of a family of cyclin gene homologues in brassica napus. | in order to investigate the role of cell division in plant development, we isolated several plant genes which encode homologues of animal and yeast cell cycle regulators known as cyclins. through the use of degenerate primers and the polymerase chain reaction (pcr) we isolated a brassica sequence which showed homology to the 'cyclin box' functional domain found within cyclin proteins. southern blot analysis indicated that brassica napus has a large number of genes containing cyclin box-related s ... | 1995 | 7888617 |
| cloning and characterization of a dihydrolipoamide acetyltransferase (e2) subunit of the pyruvate dehydrogenase complex from arabidopsis thaliana. | a cdna encoding a dihydrolipoamide acetyltransferase (e2) subunit of the pyruvate dehydrogenase complex has been isolated from arabidopsis thaliana. a cell culture cdna expression library was screened with a monoclonal antibody (jim 63) raised against nuclear matrix proteins, and four clones were isolated. one of these was 2175 base pairs in length, and it contained an open reading frame with an amino acid sequence and domain structure with strong similarity to the e2s of other eukaryotic and pr ... | 1995 | 7890655 |
| rapeseed meal-glucosinolates and their antinutritional effects. part 7. processing. | this paper surveys the effect of some technological processes based on modified traditional way of obtaining oil from rapeseeds and employing polar solvents and enzymes on the content of glucosinolates and nutritional quality of the end products. special emphasis has been put on the technologies introduced after 1980 tested mostly on a pilot scale. | 1995 | 7898576 |
| characterization of three related low-temperature-regulated cdnas from winter brassica napus. | a cdna clone, pbn115, encoding a low-temperature-regulated transcript in winter brassica napus has been isolated. northern blot analyses show that levels of transcripts hybridizing to pbn115 increase within 24 h of exposure of b. napus to low temperature, peak at 3 d, and then remain at an elevated level for the duration of the cold treatment (up to 10 weeks). transferring plants from 2 degrees c to room temperature results in the loss of detectable transcripts hybridizing to pbn115 within 1 d. ... | 1993 | 7904076 |
| comparison of the effects of two diets rich in monounsaturated fatty acids differing in their linoleic/alpha-linolenic acid ratio on platelet aggregation. | the effect of dietary linoleic/alpha-linolenic acid ratio on human platelet aggregation in vitro was investigated using low-erucic acid rapeseed oil and high-oleic acid sunflower oil as the major fat sources. in a cross-over study 20 healthy male subjects, average age 29 year (range 20-46 yr), followed experimental rapeseed oil (ro) and trisun-sunflower oil (tso) diets after their habitual diet for six weeks. subjects were provided most of the fat containing foods but were allowed to eat other f ... | 1994 | 7909389 |
| an unusual repetitive element from highly virulent isolates of leptosphaeria maculans and evidence of its transfer to a weakly virulent isolate. | a 5,238-bp repetitive dna element from a highly virulent isolate of leptosphaeria maculans has been cloned and sequenced. the element is present in approximately 80 copies per haploid genome and hybridizes to every chromosome resolved by pulse field gel electrophoresis. the sequence is composed of 66% a+t and has numerous, very short, direct and inverted repeats. no rna complementary to the element was detected in log phase cultures, and no open reading frames of significant length are present i ... | 1994 | 7912119 |
| biochemical and toxicological studies on the effect of high and low erucic acid rapeseed oil on rats. | rats were fed on diets containing rapeseed oil, either containing low or high erucic acid content as well as the hydrogenated ones for 6 weeks. body weight gain, biochemical and pathological parameters were investigated. the data showed high body weight gain for rats fed diets containing low erucic rape oil (lero) compared with those fed either the high erucic rape oil (hero), the hydrogenated or the partially hydrogenated oil diets. all rats showed non significant changes for total lipids, tota ... | 1994 | 7935743 |
| expression of the tobacco tnt1 retrotransposon promoter in heterologous species. | the expression of the tobacco (nicotiana tabacum) retrotransposon tnt1 has previously been shown to be strongly regulated and driven from the 5' long terminal repeat (ltr). we report here that the tnt1 ltr can promote activity of the beta-glucuronidase (gus) reporter gene in two heterologous species of the brassicaceae family, namely rapeseed (brassica napus) and arabidopsis thaliana. the translational ltr-gus fusion was active in transient expression studies performed with tobacco and rapeseed ... | 1994 | 7948885 |
| electron spin resonance assessment of susceptibility of roe deer (capreolus capreolus) and red deer (cervus elaphus) to oilseed rape (brassica napus) poisoning. | ex vivo studies have been carried out on roe and red deer erythrocytes using electron spin resonance spectroscopy. free radical formation in cells challenged with the brassica-derived haemolysin, dimethyldisulphide, was measured using spin trapping techniques. significantly greater amounts of radical were trapped in the roe deer cells which may relate to differences in the antioxidant profile of the two genotypes. results suggest that roe deer have a greater risk of developing oilseed rape poiso ... | 1994 | 7956119 |
| lymphatic transport of stearic acid and its effect on cholesterol transport in rats. | lymphatic transport of stearic acid, given as completely hydrogenated rapeseed oil (r10), 9 to 1 (r9) and 5 to 5 (r5) mixtures of r10, and soybean oil and completely hydrogenated tallow (t) was examined in the rat cannulated thoracic duct. r10, r9, r5, and t contained 91.4, 81.5, 46.5, and 63.6% stearic acid, respectively. a large portion of the remaining fatty acids in t was palmitic acid (31%). these fats were emulsified with bile salt and albumin, and administered via a stomach tube. lymphati ... | 1994 | 7965216 |
| effects of dietary fats on nucleoside triphosphatase activity and nuclear membrane fatty acid composition of rats during development. | the effect of various dietary fats on the nucleoside triphosphatase (ntpase) activity and nuclear membrane lipid composition of rat liver during development was assessed. rats fed a fat-free diet exhibited higher specific activity of ntpase at all ages, compared with control animals. in rats fed a sunflower oil diet, the specific activity of ntpase was also found to be highest at all ages than was observed in the control group. in contrast, animals fed the fish oil diet or peanut-rapeseed oil di ... | 1994 | 7979166 |
| effect of increasing the dietary level of rapeseed meal on intake by growing beef steers. | three experiments were conducted to determine the effects of rapeseed meal on intake by cattle. in the first experiment, the effect of increasing rapeseed meal level on intake, growth, and performance of growing beef steers was evaluated. in the second experiment, the effect of extrusion (glucosinolate reduction) on supplement intake by steers was evaluated. in the third experiment, the effect of increasing dietary rapeseed meal level on supplement intake was evaluated. in the first experiment, ... | 1994 | 7982820 |
| identification and molecular cloning of four cysteine proteinase genes from the pathogenic protozoon trichomonas vaginalis. | the parasitic protozoon trichomonas vaginalis produces multiple forms of cysteine proteinase (cp). the molecular basis for this has now been examined by cloning dna fragments encoding cps. using generic degenerate oligonucleotide primers based on two well-conserved regions within the central region of all eukaryotic cps, several polymerase chain reaction fragments were isolated from t. vaginalis genomic dna and shown to encode different cps. one fragment with a well-represented sequence was used ... | 1994 | 8000542 |
| temporal and tissue-specific regulation of a brassica napus stearoyl-acyl carrier protein desaturase gene. | the nucleotide sequence of a brassica napus stearoyl-acyl carrier protein desaturase gene (bn10) is presented. this gene is one member of a family of four closely related genes expressed in oilseed rape. the expression of the promoter of this gene in transgenic tobacco was found to be temporally regulated in the developing seed tissues. however, the promoter was also particularly active in other oleogenic tissues such as the tapetum and pollen grains. this raises the interesting question of whet ... | 1994 | 8016261 |
| regulation of an arabidopsis oleosin gene promoter in transgenic brassica napus. | progressive deletions of the 5'-flanking sequences of an arabidopsis oleosin gene were fused to beta-glucuronidase (gus) and introduced into brassica napus plants using agrobacterium-mediated transformation. the effect of these deletions on the quantitative level of gene expression, organ specificity and developmental regulation was assessed. in addition, the influence of abscisic acid (aba), jasmonic acid (ja), sorbitol and a combined aba/sorbitol treatment on gene expression was investigated. ... | 1994 | 8018869 |
| production of a toxin stemphol by stemphylium species. | five of 11 isolates of stemphylium botryosum wallr. from oilseed rape produced the phytotoxin stemphol when cultured on rice, with yields varying from 15.0 to 98.4 mg/kg, and three of them also produced the toxin on czapek dox (cd) liquid medium supplemented with cations (0.12-0.31 mg/l) and potato-dextrose (pd) broth (0.37-1.47 mg/l). in liquid cultures about 95% of stemphol was found in the mycelium, and toxin production was slightly increased when cultures were exposed to near uv light rather ... | 1994 | 8032689 |
| isolation of cdna clones for genes that are expressed during leaf senescence in brassica napus. identification of a gene encoding a senescence-specific metallothionein-like protein. | cdna clones representing genes that are expressed during leaf senescence in brassica napus were identified by differential screening of a cdna library made from rna isolated from leaves at different stages of senescence. the expression of these genes at different stages of leaf development was examined by northern blot analysis, and several different patterns of expression were observed. one of the clones, lsc54, represented a gene that is expressed at high levels during leaf senescence. analysi ... | 1994 | 8058836 |
| cloning of a higher-plant plastid omega-6 fatty acid desaturase cdna and its expression in a cyanobacterium. | oligomers based on amino acids conserved between known plant omega-3 and cyanobacterium omega-6 fatty acid desaturases were used to screen an arabidopsis cdna library for related sequences. an identified clone encoding a novel desaturase-like polypeptide was used to isolate its homologs from glycine max and brassica napus. the plant deduced amino acid sequences showed less than 27% similarity to known plant omega-6 and omega-3 desaturases but more than 48% similarity to cyanobacterial omega-6 de ... | 1994 | 8066133 |
| molecular cloning of a cdna from brassica napus l. for a homologue of acyl-coa-binding protein. | a cdna encoding an acyl-coa-binding protein (acbp) homologue has been cloned from a lambda gt11 library made from mrna isolated from developing seeds of oilseed rape (brassica napus l.). the derived amino acid sequence reveals a protein 92 amino acids in length which is highly conserved when compared with acbp sequences from yeast, cow, man and fruit fly. southern blot analysis of brassica napus genomic dna revealed the presence of 6 genes, 3 derived from the brassica rapa parent and 3 from bras ... | 1994 | 8075407 |
| molecular cloning and expression of a cdna encoding the proliferating cell nuclear antigen from brassica napus (oilseed rape). | a cdna clone encoding the proliferating cell nuclear antigen (pcna) has been isolated from a brassica napus apical meristem cdna library. the putative full-length cdna contains an open reading frame of 1004 nucleotides, which predicts a protein of 263 amino acids (m(r) = 29,231). sequence analysis has revealed that the plant pcna exhibits 81.6% amino acid similarity with the human pcna. genomic southern blot analysis indicates the presence of at least two copies of pcna per genome. the b. napus ... | 1993 | 8102346 |
| effect of age and alpha-linolenic acid deficiency on delta 6 desaturase activity and liver lipids in rats. | the combined effects of age and of diet deficient in n-3 fatty acids on delta 6 desaturation of linoleic acid and on lipid fatty acid composition were studied in the liver of the rat at 2, 6, 12, 18 and 24 mon of age. the profiles of delta 6 desaturase activity and fatty acid composition were studied in the deficient rats refed, at these different ages, either with 18:3n-3 (mixture of peanut and rapeseed oils) or with 20:5n-3 + 22:6n-3 (fish oil) diets for 2, 4, 8 or 12 wk. results showed that t ... | 1993 | 8102769 |
| a seed-specific brassica napus oleosin promoter interacts with a g-box-specific protein and may be bi-directional. | in brassica napus, oleosins are expressed at high levels in the seed during the latter stages of embryo development. the cis-acting regulatory properties of an 872 bp promoter fragment of a b. napus oleosin gene were examined by analysis of beta-glucuronidase (gus) expression in transgenic tobacco plants containing an oleosin promoter-gus transcriptional fusion. the reporter gene was expressed at high levels only in seeds, specifically in embryo and endosperm tissue and regulated throughout seed ... | 1994 | 8111035 |
| enod8, a novel early nodule-specific gene, is expressed in empty alfalfa nodules. | the alfalfa enod8 nodule-specific gene is expressed in empty nodules elicited by exopolysaccharide-deficient rhizobium meliloti, and it is expressed early in nodule development. an enod8 cdna was sequenced and found to encode a novel product. its deduced polypeptide sequence was found to be similar to the nonproline-rich domains of the putative polypeptides encoded by a class of anther-specific genes from arabidopsis thaliana and brassica napus. the role of the enod8 gene product is predicted to ... | 1993 | 8118054 |
| toxic oil stimulates collagen synthesis acting at a pretranslational level in cultured fat-storing cells. | the toxic oil syndrome appeared in spain in 1981 as a result of ingestion of rapeseed oil denatured with aniline. some patients developed scleroderma-like skin lesions and liver cirrhosis. mechanisms of these fibrotic lesions are not known. the present study was designed to investigate the effect of toxic oils on collagen metabolism. | 1994 | 8119541 |
| backfat and carcass composition of piglets fed milk replacers containing vegetable oil compared with sow-reared piglets. | the carcass composition of piglets fed artificial milk was compared to sow-reared piglets. the artificial milk diets contained 25%, by weight, soybean oil or mixtures of canola and high erucic acid rapeseed oil. both the total lipid and nitrogen (apparent) digestibility of the artificial milk diets was high, even when the dietary oil contained high levels of erucic acid. sow-reared animals were matched with the piglets receiving the artificial milk by sex and live body weight. on both a relative ... | 1994 | 8129839 |
| purification, inhibitory properties, amino acid sequence and identification of the reactive site of a new serine proteinase inhibitor from oil-rape (brassica napus) seed. | a new serine proteinase inhibitor, rapeseed trypsin inhibitor (rti), has been isolated from rapeseed (brassica napus var. oleifera) seed. the protein inhibits the catalytic activity of bovine beta-trypsin and bovine alpha-chymotrypsin with apparent dissociation constants of 3.0 x 10(-10) m and 4.1 x 10(-7) m, at ph 8.0 and 21 degrees c, respectively. the stoichiometry of both proteinase-inhibitor complexes is 1:1. the amino acid sequence of rti consists of 60 amino acid residues, corresponding t ... | 1994 | 8143882 |
| identification of conserved domains in the delta 12 desaturases of cyanobacteria. | cyanobacterial genes for enzymes that desaturate fatty acids at the delta 12 position, designated desa, were isolated from synechocystis pcc6714, synechococcus pcc7002 and anabaena variabilis by cross-hybridization with a dna probe derived from the desa gene of synechocystis pcc6803. the genes of synechocystis pcc6714, synechococcus pcc7002 and a. variabilis encode proteins of 349, 347 and 350 amino acid residues, respectively. the transformation of synechococcus pcc7942 with the desa genes from ... | 1994 | 8155883 |
| effect of body weight on net energy value of feeds for growing pigs. | digestible (de), metabolizable (me), and net (ne) energy values of seven diets were measured in castrated male pigs of 45 (stage 1), 100 (stage 2), or 150 (stage 3) kg bw. diets were prepared from a basal diet supplemented with cornstarch, or sucrose, or a protein mixture (referred to here as protein), or rapeseed oil, or a mixture of fibrous ingredients (referred to as fiber), or rapeseed oil+fiber. diets were fed at similar levels (x maintenance) at the three stages. heat production at differe ... | 1994 | 8181981 |
| suppression of a temperature-sensitive cdc33 mutation of yeast by a multicopy plasmid expressing a drosophila ribosomal protein. | the saccharomyces cerevisiae cdc33ts4-2 mutant produces a temperature-sensitive allele of the cap-binding subunit of eukaryotic initiation factor-4f (also termed eif-4e). from a drosophila cdna library constructed in a multicopy yeast shuttle vector, a clone was isolated which restored the ability to grow at elevated temperature to cdc33ts4-2 cells. the rescuing drosophila clone encodes a small ribosomal subunit protein, which we name s15a based on its molecular weight and similarity with the br ... | 1994 | 8182070 |
| rate of alteration of hepatic mixed-function oxidase system in rats fed different dietary fats. | studies were carried out to evaluate and relate the rate of alteration in mixed-function oxidase system with the changes of the fatty acid composition of rat microsomes induced by different dietary lipids. male weanling rats were fed from day 21 to 120 with a commercial rat diet or a semisynthetic diet containing no fat or 10% fat consisting of peanut-rapeseed oil, sunflower oil, or salmon oil. in rats fed a fat-free diet, the cytochrome p-450 concentration and aniline hydroxylase, aminopyrine n ... | 1993 | 8192891 |
| effect of different dietary supplemental fats and oils on the tissue fatty acid composition and growth of female broilers. | 1. a study was undertaken to examine the effect of supplementation of diets with fats of different chain length and degree of unsaturation on the performance, carcase characteristics and tissue fatty acid composition of broilers. 2. three hundred and sixty 19-day-old female broilers were fed on diets containing supplemental fat/oil (50 g/kg) in the form of beef tallow (bt) (diet t-t), soyabean oil (sbo) (diet s-s), rapeseed oil (rso) (diet r-r), marine oil (mo) (diet m-m) or binary mixtures (0.5 ... | 1994 | 8199880 |
| effect of substrate on the production of antifungal volatiles from bacillus subtilis. | an antibiotic-producing strain of bacillus subtilis has been shown to produce potent antifungal volatiles (afv). these volatiles are active against a range of fungal species and are produced on a range of growth media and in loam-based compost. in vitro antifungal volatile activity on nutrient agar is enhanced with the addition of d-glucose, complex carbohydrates and peptones. the addition of l-glucose led to significantly less afv activity than comparable levels of d-glucose. growth studies in ... | 1994 | 8200865 |
| dietary diacylglycerol-dependent reduction in serum triacylglycerol concentration in rats. | the effects of dietary diacylglycerol consisting of 1,3 (65.2%) and 1,2 species (32.6%) and triacylglycerol (rapeseed oil) on the serum and hepatic lipid profiles were compared in the rat. the fatty acid composition was similar between these dietary lipids. the dietary acylglycerols were added to the experimental diets so as to provide the same amounts of fatty acids (9.39%). dietary diacylglycerol compared with triacylglycerol significantly reduced concentrations of serum triacylglycerol at 17 ... | 1993 | 8215235 |
| lipolytic activity and degradation of rapeseed oil and rapeseed by spoilage fungi. | aspergillus, eurotium and penicillium spp. from rapeseed were able to grow and produce lipases over a range of water activities (aw) at both 15 and 25 degrees c on tributyrin agar. the ability to produce lipases was not directly related to growth rate. the clearing zone: growth rate ratios gave lipase indices which varied markedly between test fungi and with aw. the fungi with the highest indices were aspergillus candidus and aspergillus versicolor and penicillium expansum and penicillium hordei ... | 1993 | 8217518 |
| alteration in 5'-nucleotidase activities and composition of liver and brain microsomes of developing rats fed different dietary fats. | four groups of male weanling rats were fed during three months, diets different in the nature of fats and the activity of 5' nucleotidase and fatty acid composition of brain and liver microsomes were studied. group a were fed a standard commercial diet, group b a fat free-diet and group c and d a fat free-diet, containing respectively 10% of peanut-rapeseed oil and 10% of salmon oil. in brain and liver microsomes, 5'-nucleotidase activity increased throughout the development for all diets (excep ... | 1993 | 8220257 |
| lipid accumulation in isolated perfused rat hearts has no apparent effect on mechanical function or energy metabolism as measured by 31p nmr. | male sprague-dawley rats were fed diets that contained 20% by weight soybean oil or rapeseed oil (21% and 43% erucic acid) for 7 days. the rapeseed oil diets increased the cardiac triacylglycerol content 5-fold and 25-fold, respectively, above control values. hearts were removed from the animals and perfused with modified krebs-henseleit buffer at 37 degrees c. the calculated rate-pressure product was used as a measure of contractile function. 31p nmr spectra were acquired throughout a protocol ... | 1993 | 8228640 |
| deletion analysis of a 2s seed storage protein promoter of brassica napus in transgenic tobacco. | the promoter and upstream region of the brassica napus 2s storage protein napa gene were studied to identify cis-acting sequences involved in developmental seed-specific expression. fragments generated by successive deletions of the 5' control region of the napa gene were fused to the reporter gene beta-glucuronidase (gus). these constructs were used to transform tobacco leaf discs. analyses of gus activities in mature seeds from the transformed plants indicated that there were both negatively a ... | 1993 | 8251622 |
| molecular characterization of two cdnas from sinapis alba l. expressed specifically at an early stage of tapetum development. | flower formation in the long-day plant sinapis alba is strictly dependent on an inductive light treatment. differential screening of an apex cdna library prepared 10 days after flower induction against cdnas from vegetative apices has identified two cdna clones, psfd10.35 and psfd10.44, which represent transcripts expressed transiently between day 10 and day 20 after induction and which disappear before flowers reach maturity. the corresponding full-length cdnas which were isolated, satap35 and ... | 1993 | 8252070 |
| genes for ribosomal proteins s3, l16, l5 and s14 are clustered in the mitochondrial genome of brassica napus l. | we have cloned and sequenced an 8.9-kb mitochondrial-dna fragment from rapeseed (brassica napus l.). the nucleotide sequence indicates a gene cluster that encodes four ribosomal proteins (s3, l16, l5, s14), two trna genes (trnd, trnk), and the 5' region of the cob gene. the arrangement of these seven genes is trnd-trnk-rps3-rpl16-rpl5-rps14-cob. the rps3 and rpl16 frames overlap by 131 bp. the rpl5 and rps14 genes are separated by a 4-bp spacer. a 1474-basepair intron is located in the rps3 gene ... | 1993 | 8252643 |
| purification and sequencing of radish seed calmodulin antagonists phosphorylated by calcium-dependent protein kinase. | a family of radish (raphanus sativus) calmodulin antagonists (rcas) was purified from seeds by extraction, centrifugation, batch-wise elution from carboxymethyl-cellulose, and high performance liquid chromatography (hplc) on an sp5pw cation-exchange column. this rca fraction was further resolved into three calmodulin antagonist polypeptides (rca1, rca2, and rca3) by denaturation in the presence of guanidinium hcl and mercaptoethanol and subsequent reverse-phase hplc on a c8 column eluted with an ... | 1993 | 8278508 |
| two related, low-temperature-induced genes from brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene. | in order to identify genes involved in cold acclimation, we have constructed a cdna library from brassica napus (cv. samouraï) cold-acclimated etiolated seedlings. by differential screening, a cdna clone named pbnc24 (brassica napus cold), corresponding to a new cold-inducible plant gene, was isolated. northern blot hybridizations using total rna from acclimated and unacclimated seedlings confirmed that bnc24 represents a cold-regulated gene. in contrast with a number of cold-inducible plant gen ... | 1993 | 8292785 |
| transgenic plants that express genes including the 3' untranslated region of the turnip yellow mosaic virus (tymv) genome are partially protected against tymv infection. | in order to evaluate new possibilities for protecting plants against virus infection by interference with viral replication, two chimeric genes were constructed in which the (+) strand 3'-terminal 100 nucleotides (nt) of the noncoding region of the turnip yellow mosaic virus (tymv) genome were placed downstream from the sense or antisense cat coding region. the two chimeric genes were then introduced into the genome of rapeseed (brassica napus) using an agrobacterium rhizogenes vector system. pl ... | 1993 | 8294045 |
| dietary monounsaturated fatty acids enhance cholesterol efflux from human fibroblasts. relation to fluidity, phospholipid fatty acid composition, overall composition, and size of hdl3. | this study was designed to determine whether modifications induced by dietary fats on the high-density lipoprotein3 (hdl3) physicochemical characteristics could affect cholesterol efflux and intracellular cholesterol content, leading to upregulation of low-density lipoprotein (ldl) receptor activity from cultured fibroblasts. serum hdl3s were obtained from 12 healthy women aged 26 to 49 years who adhered to four 7-week isocaloric diets containing 30% of the caloric intake as fat. of the total ca ... | 1993 | 8318513 |
| 1h-nmr spectroscopy can accurately quantitate the lipolysis and oxidation of cardiac triacylglycerols. | triacylglycerol metabolism in isolated, perfused hearts from rats fed a diet containing 20% rapeseed oil (rso) was studied using 1h-nmr spectroscopy. rso-induced elevation in cardiac triacylglycerols is associated with an increase in the peak area of fatty acid 1h-nmr resonances. the ratio of methyl, gamma-methylene or methylene protons adjacent to a carbon-carbon double bond to the number of methylene protons in these hearts measured by 1h-nmr spectroscopy gives values similar to those derived ... | 1993 | 8343541 |
| major chromosomal length polymorphisms are evident after meiosis in the phytopathogenic fungus leptosphaeria maculans. | chromosomal dna of australian field-isolates of the phytopathogenic ascomycete leptosphaeria maculans was resolved by pulsed-field gel electrophoresis. all isolates examined had highly variable karyotypes. ascospores (sexual spores) derived from single pseudothecia (sexual fruiting bodies) isolated from brassica napus (oilseed rape) stubble were analyzed. in two tetrads four distinct karyotypes were observed, with only one chromosomal dna band in common to all the members of each tetrad. althoug ... | 1993 | 8358816 |
| effect of an n-3 fatty acid-deficient diet on the adenosine-dependent melatonin release in cultured rat pineal. | we studied the effect of a diet deficient in n-3 fatty acids on the adenosine-dependent melatonin release from cultured rat pineal gland after stimulation by 5'-n-ethylcarboxamidoadenosine (neca), an a2 adenosine agonist. experiments were conducted with 2-month-old rats raised on semipurified diets containing either peanut oil (n-3 deficients) or peanut plus rapeseed oil (controls). the proportion of docosahexaenoic acid (22:6 n-3) in the pineal total lipid fraction and in phosphatidylcholine an ... | 1993 | 8360673 |
| regulated expression of the rat medium chain hydrolase gene in transgenic rape seed. | medium chain hydrolase (mch) is an enzyme which regulates the chain length of fatty acid synthesis specifically in the mammary gland of the rat. during lactation, mch interacts with fatty acid synthase (fas) to cause premature release of acyl chains, thus providing medium chain fatty acids for synthesis of milk fat. in this study we have investigated the ability of rat mch to interact with the phylogenetically more distant fas structure present in plant systems and to cause a perturbation of fat ... | 1993 | 8364602 |
| ruminal lipid balance and intestinal digestion by dairy cows fed calcium salts of rapeseed oil fatty acids or rapeseed oil. | the effects of supplemental calcium salts of rapeseed oil fatty acids (fa) and rapeseed oil on ruminal metabolism and apparent digestibility of lipids in the small intestine were studied using three multiparous holstein x friesian cows in a 3 x 3 latin square design. cows fitted with ruminal, duodenal, and ileal cannulas were fed restricted amounts of a control diet (c) containing 65% corn silage and 35% concentrate mix or diet c with supplemental calcium salts of rapeseed oil fa (s) or diet c s ... | 1993 | 8376250 |
| electrotransfection of turnip yellow mosaic virus rna into brassica leaf protoplasts and detection of viral rna products with a non-radioactive probe. | we describe here a convenient and efficient system for studying turnip yellow mosaic virus (tymv) replication in leaf protoplasts. inoculation of rapeseed (brassica napus) or chinese cabbage (b. sinensis) protoplasts was achieved via electroporation, and sensitive detection of viral rna products was performed by northern blot analyses using a non-radioactive digoxigenin-labelled cdna probe. virus replication was detected when 1.5 x 10(6) rapeseed protoplasts were inoculated with 20 ng of tymv rn ... | 1993 | 8376966 |
| organic matter and nitrogen digestion by dairy cows fed calcium salts of rapeseed oil fatty acids or rapeseed oil. | the aim of this experiment was to study the effects on digestion of a supply of ca salts of vegetable oil as a fat source for ruminants. total tract digestibility and ruminal digestion were studied using three ruminally and duodenally fistulated dairy cows in a latin square design. diets compared were a control diet (c) based on corn silage and concentrate, and the c diet supplemented with calcium salts of rapeseed oil (s) or supplemented with rapeseed oil (o). organic matter digestibility was l ... | 1993 | 8382674 |
| protein phosphatases in higher plants: multiplicity of type 2a phosphatases in arabidopsis thaliana. | two dna fragments, ap-1 and ap-2, encoding amino acid sequences closely related to ser/thr protein phosphatases were amplified from arabidopsis thaliana genomic dna. fragment ap-1 was used to screen a. thaliana cdna libraries and several positive clones were isolated. clones ep8a and ep14a were sequenced and found to encode almost identical proteins (97% identity). both proteins are 306 amino acids in length and are very similar (79-80% identity) to the mammalian isotypes of the catalytic subuni ... | 1993 | 8382968 |
| molecular cloning and expression of a map kinase homologue from pea. | the cdc2 kinases are important cell cycle regulators in all eukaryotes. map kinases, a closely related family of protein kinases, are involved in cell cycle regulation in yeasts and vertebrates, but previously have not been documented in plants. we used pcr to amplify brassica napus dna sequences using primers corresponding to amino sequences that are common to all known protein kinases. one sequence was highly similar to kss1, a map kinase from saccharomyces cerevisiae. this sequence was used t ... | 1993 | 8388749 |
| isolation and characterization of a phosphoprotein phosphatase type 2a gene from alfalfa. | phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. we report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2a (pp2ams) cdna. the predicted protein sequence shows high similarity to pp2a from brassica napus, rabbit and drosophila. no changes in pp2ams mrna abundance during the cell cycle were found. during growth of a batch cell culture, mrna levels decreased gradually. in planta, all organs contained pp2a transcripts but maxi ... | 1993 | 8393512 |
| morphometric analysis demonstrates that metabolically active cardiac triglycerides are 1h nmr visible. | the relationship between myocardial triglyceride content and 1h nmr visible fatty acid resonance intensity was investigated. hearts from rats fed a 20% rapeseed oil diet contained markedly increased levels of triglycerides as judged by thin layer chromatographic analysis. this elevation in cardiac triglycerides was associated with sharp increases in the cell volume occupied by lipid droplets and in 1h nmr visible fatty acid resonances. spin-lattice and spin-spin relaxation times of the 1h nmr vi ... | 1993 | 8397304 |
| molecular cloning and characterization of anther-preferential cdna encoding a putative actin-depolymerizing factor. | a cdna clone, lmp131a, which is preferentially expressed in mature anther was isolated from a lily cdna library. northern blot analysis and plaque hybridization experiments showed that the lmp131a mrna is present at ca. 0.3% of the mrna in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. the clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factor ... | 1993 | 8425049 |
| structure and evolution of a highly repetitive dna sequence from brassica napus. | a hind iii family of highly repetitive dna sequences, canrep (canola repeat), was cloned from the nuclear dna of canola (brassica napus cv. westar). the basic units of this family of repeats consists of 176 bp and are arranged in clusters of tandem direct repeats. each canrep repeat is composed of three related subrepeats of ca. 60 bp. each subrepeat contains two inverted repeats of about 23 bp and another unrelated sequence of about 12 bp. based on the internal structure, a possible scheme for ... | 1993 | 8425054 |
| effect of dietary calcium level on mineral and trace element utilization from a rapeseed (brassica napus l.) diet fed to ileum-fistulated pigs. | the bioavailability of intrinsic minerals in rapeseed (brassica napus l.) meal was studied in growing, fistulated pigs. furthermore, the effect on mineral availability of an extrinsic ca supply to this diet, high in phytate, was observed. comparisons between small intestinal and total gastrointestinal absorption were accomplished for minerals and other dietary components. n retention increased as the dietary ca intake increased, but dropped, in general significantly, throughout the experimental ... | 1993 | 8457529 |
| two classes of isocitrate lyase genes are expressed during late embryogeny and postgermination in brassica napus l. | we have analyzed the structure of genes encoding the glyoxylate cycle enzyme isocitrate lyase from brassica napus l. and their expression during embryogeny and postgermination. restriction mapping, nucleotide sequence, and dna gel blot hybridization analyses of cdna and genomic clones indicated that there are approximately six isocitrate lyase genes in the b. napus genome that can be divided into at least two subfamilies based upon their divergence in 5' and 3' untranslated regions. we showed pr ... | 1993 | 8479425 |
| brain phospholipids as dietary source of (n-3) polyunsaturated fatty acids for nervous tissue in the rat. | in a previous work, we calculated the dietary alpha-linolenic requirements (from vegetable oil triglycerides) for obtaining and maintaining a physiological level of (n-3) fatty acids in developing animal membranes as determined by the cervonic acid content [22:6(n-3), docosahexaenoic acid]. the aim of the present study was to measure the phospholipid requirement, as these compounds directly provide the very long polyunsaturated fatty acids found in membranes. two weeks before mating, eight group ... | 1993 | 8492115 |
| [effects of dietary fats of vegetable and animal origin on lipid synthesis in pigs]. | we have compared the effect of dietary fats, unsaturated (rapeseed oil: ro) or saturated (cow milk: cm), on growth performance, lipogenesis and fatty acid composition of backfat. both group had the same food intake and growth performance. nevertheless, the percentage of body fat increases with the diet ro (p < 0.05). lipogenesis enzyme activities of backfat were greater with ro than with mc. then, it appeared that pigs fed diets containing high level of unsaturated fat with long-chain fatty acid ... | 1995 | 8521081 |
| common themes in redox chemistry emerge from the x-ray structure of oilseed rape (brassica napus) enoyl acyl carrier protein reductase. | enoyl acyl carrier protein reductase (enr) catalyzes the nad(p)h-dependent reduction of trans-delta 2-enoyl acyl carrier protein, an essential step in de novo fatty acid biosynthesis. plants contain both nadh-dependent and separate nadph-dependent enr enzymes which form part of the dissociable type ii fatty acid synthetase. highly elevated levels of the nadh-dependent enzyme are found during lipid deposition in maturing seeds of oilseed rape (brassica napus). | 1995 | 8535786 |
| structural requirements of oleosin domains for subcellular targeting to the oil body. | we have investigated the protein domains responsible for the correct subcellular targeting of plant seed oleosins. we have attempted to study this targeting in vivo using "tagged" oleosins in transgenic plants. different constructs were prepared lacking gene sequences encoding one of three structural domains of natural oleosins. each was fused in frame to the escherichia coli uid a gene encoding beta-glucuronidase (gus). these constructs were introduced into brassica napus using agrobacterium-me ... | 1995 | 8539295 |
| deletion analysis of the brassica napus cruciferin gene cru 1 promoter in transformed tobacco: promoter activity during early and late stages of embryogenesis is influenced by cis-acting elements in partially separate regions. | to define sequences in the cruciferin gene cru1 promoter of importance for expression, tobacco (nicotina tabacum l.) plants were transformed with constructs in which the cru1 promoter, in front of the intact cru1 structural gene, was truncated at -1216, -974, -736, -515, -306, -46 and -17 bp relative to the cap-site. cru1 expression in tobacco seeds was studied by northern analysis, western analysis and in-situ hybridizations. comparisons of the northern analysis of rna from tobacco seeds harves ... | 1995 | 8547815 |
| biotransformation of 3-(phenylamino)-1,2-propanediol to 3-(phenylamino)alanine: a chemical link between toxic oil syndrome and eosinophilia-myalgia syndrome. | during late 1989, the eosinophilia-myalgia syndrome (ems) developed as an epidemic in the united states, with numerous additional cases reported in several other countries worldwide. eight years earlier, a closely-related disease, the toxic oil syndrome (tos), occurred in spain as a massive food-borne epidemic. although ems was linked to the ingestion of tainted l-tryptophan, and tos to aniline-denatured rapeseed oil, the etiologic agent(s) responsible for both diseases remains undetermined. con ... | 1995 | 8555405 |
| evaluation of the allergic/irritant potential of air pollutants: detection of proteins modified by volatile organic compounds from oilseed rape (brassica napus ssp. oleifera) using electrospray ionization-mass spectrometry. | upward trends in allergy and asthma rates have been reported in most western societies, including the uk, where around 15-20% of the population now suffer from allergy or asthma. scientific proof of the causes of these increases relies on accurate assessment of exposure and standardized diagnostic tests, such as for specific ige in blood serum and skin testing. for many air pollutants it has proven difficult to assess an individual's exposure outside an occupational environment and reliable test ... | 1995 | 8556570 |
| the combination of gilbert/maxam chemical sequencing and the dideoxynucleotide chain termination approach facilitates the construction of species specific pcr-primers based on diagnostic rapd bands. | the randomly amplified polymorphic dna technique (rapd) is a modification of pcr that uses short, arbitrarily generated single primers to amplify genomic dna. amplified dna-fragments are often polymorphic and can be used as individual, population- or species-specific markers. because the rapd technique requires a very high degree of reproducibility at the instrumentation level and with regard to buffer conditions, we propose to synthesize highly specific conventional pcr primers, the sequence of ... | 1995 | 8564365 |