| ribosomal protein s1 in archaea. | cell extracts and ribosomes of thermoacidophilic and halophilic archaebacteria were analysed by immunoblotting to detect ribosomal protein s1 homologues. antisera to e. coli s1, the n-terminal ribosome binding domain (f2a) and the central and cooh-terminal nucleic acid binding domain (s1f1) of the protein were used. the results show that the thermoacidophilic archaebacterium sulfolobus acidocaldarius contains a protein with molecular weight of 66,000 that cross-reacts with anti-s1f1. however, th ... | 1994 | 7833831 |
| a seven-iron ferredoxin from the thermoacidophilic archaeon desulfurolobus ambivalens. | a seven-iron ferredoxin was isolated from aerobically grown cells of the hyperthermoacidophilic archaeon desulfurolobus ambivalens (dsm 3772). the protein is monomeric, with an apparent molecular mass of 15 kda and contains 7 iron atoms/molecule. the n-terminal sequence shows a large similarity (70% identity) with that of the ferredoxin isolated from the archaeon sulfolobus acidocaldarius. the epr characteristics in both the native (oxidized) and dithionite-reduced states of this protein allowed ... | 1995 | 7851403 |
| introduction of additional charges as an aid in protein purification: isolation of elongation factor 2 from sulfolobus acidocaldarius by preparative isoelectric focusing before and after adp-ribosylation. | diphtheria toxin catalyzes the adp-ribosylation of elongation factor 2 (ef-2) in eukaryotes and archae-bacteria. as the reaction is strictly ef-2 specific and introduces two negative charges into the molecule, the resulting shift in the isoelectric point (pi) by 0.2 ph units was used to establish a new purification method for ef-2 from sulfolobus acidocaldarius. the cells were lysed with dithiothreitol at ph 9 and ef-2 was purified by ammonium sulfate precipitation, gel filtration on sephadex g- ... | 1994 | 7858424 |
| a physical map of the sulfur-dependent archaebacterium sulfolobus acidocaldarius 7 chromosome. | a chromosomal map of the sulfur-dependent thermoacidophilic archaebacterium sulfolobus acidocaldarius 7 was constructed with four restriction enzymes: noti, bsshii, rsrii, and eagi. the map indicated that the chromosome is a single circular dna of 2,760 +/- 20 kb (mean +/- standard error of the mean). rrna genes were also mapped. they were located at one site in the genome. | 1993 | 7680342 |
| purification and characterization of the rieske iron-sulfur protein from the thermoacidophilic crenarchaeon sulfolobus acidocaldarius. | the previously detected rieske iron-sulfur protein from the membranes of the thermoacidophile sulfolobus acidocaldarius [anemüller, s., et al. (1993) febs lett. 318, 61-64] was purified to electrophoretic homogeneity and the n-terminal amino acids determined. the apparent molecular weight was estimated to be 32 kda. the reduced protein displays a rhombic epr spectrum with gxyz = 1.768, 1.895, 2.035. the average g-value of 1.902 is typical for nitrogen ligand-containing clusters. epr spin quantif ... | 1995 | 7867807 |
| expression of a synthetic gene encoding p2 ribonuclease from the extreme thermoacidophilic archaebacterium sulfolobus solfataricus in mesophylic hosts. | this work reports the molecular cloning and expression of a synthetic gene encoding p2, a 7-kda ribonuclease (rnase) previously isolated in our laboratory from the archaebacterium sulfolobus solfataricus [fusi et al., eur. j. biochem. 211 (1993) 305-310]. the p2-encoding synthetic gene was expressed in e. coli and in saccharomyces cerevisiae. the recombinant (re-) protein was produced to approx. 1.5% of the total protein content in s. cerevisiae using the galactose-inducible gal1 promoter and to ... | 1995 | 7867957 |
| [preservation of acidophilic bacteria]. | | 1994 | 7873107 |
| an 8.5-kda ribonuclease from the extreme thermophilic archaebacterium sulfolobus solfataricus. | protein p3, a ribonuclease we previously isolated from the archaebacterium sulfolobus solfataricus [p. fusi et al. (1993) eur. j. biochem. 211, 305-310], was subjected to complete amino acid sequencing. it consisted of 75 residues, with a calculated m(r) of 8582, a pi of 10.1, and had some degree of monomethylation at lys-4 and lys-6. p2, a previously sequenced, 62-residue ribonuclease from the same organism, had an identical sequence for 57 consecutive residues starting from the n-terminus. p2 ... | 1995 | 7875327 |
| characterization of the rnase p rna of sulfolobus acidocaldarius. | rnase p is the ribonucleoprotein enzyme that cleaves precursor sequences from the 5' ends of pre-trnas. in bacteria, the rna subunit is the catalytic moiety. eucaryal and archaeal rnase p activities copurify with rnas, which have not been shown to be catalytic. we report here the analysis of the rnase p rna from the thermoacidophilic archaeon sulfolobus acidocaldarius. the holoenzyme was highly purified, and extracted rna was used to identify the rnase p rna gene. the nucleotide sequence of the ... | 1993 | 7688716 |
| chromosomal organization and nucleotide sequence of the fus-gene encoding elongation factor 2 (ef-2) of the hyperthermophilic archaeum pyrococcus woesei. | a pyrococcus woesei ecori dna fragment (3400 bp) harbouring the gene fus for elongation factor 2 (ef-2) was cloned and almost completely sequenced. unlike methanococcus vannielii (which displays the 'str operon'-like fus and tuf gene context, 5'-rps12-rps7-fus-tuf-3'), and similar to sulfolobus acidocaldarius and desulfurococcus mobilis, the pyrococcus fus gene (732 codons) is unlinked to the rps and tuf genes, and is immediately followed (57 bp intergenic spacing) by an orf of 106 codons. both ... | 1995 | 7896082 |
| chromosomal organization and nucleotide sequence of the genes for elongation factors ef-1 alpha and ef-2 and ribosomal proteins s7 and s10 of the hyperthermophilic archaeum desulfurococcus mobilis. | the desulfurococcus mobilis genes fus (encoding ef-2) and tuf (for ef-1 alpha) were cloned and sequenced together with genes for ribosomal proteins s10 (rps10) and s7 (rps7). unlike methanococcus, which displays the bacterial-like fus and tuf gene context 5'-rps12-rps7-fus-tuf-3', and similar to sulfolobus and pyrococcus, the desulfurococcus fus gene (734 codons) has a distinct chromosomal location. moreover, tuf (441 codons) is the promoter-proximal unit of a three-gene cluster comprising the g ... | 1995 | 7898436 |
| purification and characterization of a maltase from the extremely thermophilic crenarchaeote sulfolobus solfataricus. | a soluble maltase (alpha-glucosidase) with an apparent subunit mass of 80 kda was purified to homogeneity from sulfolobus solfataricus. the enzyme liberates glucose from maltose and malto-oligomers. maximal activity was observed at 105 degrees c, with half-lives of 11 h (85 degrees c), 3.0 h (95 degrees c), and 2.75 h (100 degrees c). the enzyme was generally resistant to proteolysis and denaturants including aliphatic alcohols. n-propanol treatment at 85 degrees c increased both km and vmax for ... | 1995 | 7814342 |
| evolutionary relationships of bacterial and archaeal glutamine synthetase genes. | glutamine synthetase (gs), an essential enzyme in ammonia assimilation and glutamine biosynthesis, has three distinctive types: gsi, gsii and gsiii. genes for gsi have been found only in bacteria (eubacteria) and archaea (archaebacteria), while gsii genes only occur in eukaryotes and a few soil-dwelling bacteria. gsiii genes have been found in only a few bacterial species. recently, it has been suggested that several lateral gene transfers of archaeal gsi genes to bacteria may have occurred. in ... | 1994 | 7916055 |
| the thermosome of thermoplasma acidophilum and its relationship to the eukaryotic chaperonin tric. | a high molecular-mass protein complex from the archaebacterium thermoplasma acidophilum, referred to here as the 'thermosome', is built from two subunits (m(r) 58 and 60). the thermosome has been purified to homogeneity. the molecular mass of the native complex was determined to be 1061 +/- 30 da by scanning transmission electron microscopy. it shows a weak atpase activity and is able to bind denatured polypeptides. averages obtained from electron micrographs of negatively stained molecules in t ... | 1995 | 7867646 |
| acquired thermotolerance and heat shock proteins in thermophiles from the three phylogenetic domains. | thermophilic organisms from each of the three phylogenetic domains (bacteria, archaea, and eucarya) acquired thermotolerance after heat shock. bacillus caldolyticus grown at 60 degrees c and heat shocked at 69 degrees c for 10 min showed thermotolerance at 74 degrees c, sulfolobus shibatae grown at 70 degrees c and heat shocked at 88 degrees c for 60 min showed thermotolerance at 95 degrees c, and thermomyces lanuginosus grown at 50 degrees c and heat shocked at 55 degrees c for 60 min showed th ... | 1994 | 7928981 |
| purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon sulfolobus solfataricus. purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds. | 5'-methylthioadenosine phosphorylase from sulfolobus solfataricus, a thermoacidophilic archaeon optimally growing at 87 degrees c, has been purified to homogeneity. reducing agents are not required for catalytic activity. the enzyme has a molecular mass of 160 kda and is composed of six apparently identical subunits of 27 kda. the nh2-terminal sequence shows high homology (50%) with the nh2-terminal sequence of escherichia coli purine nucleoside phosphorylase. physicochemical and kinetic feature ... | 1994 | 7929153 |
| the molecular chaperonin tf55 from the thermophilic archaeon sulfolobus solfataricus. a biochemical and structural characterization. | the purification and characterization of a new type of thermostable chaperonin from the archaebacterium sulfolobus solfataricus is described. the chaperonin forms a hetero-oligomeric complex of two different, but closely related, subunits, which we have assigned tf55-alpha and tf55-beta. their n-terminal sequences and amino acid residue compositions are reported. two-dimensional projections of the chaperonin have been reconstructed from electron microscopy images, showing a 9-fold symmetrical co ... | 1994 | 7932699 |
| indole-3-glycerol-phosphate synthase from sulfolobus solfataricus as a model for studying thermostable tim-barrel enzymes. | indole-3-glycerol-phosphate synthase, a thermophilic and thermostable enzyme from the archaeon sulfolobus solfataricus, was purified and characterized. the sequence of the thermophilic enzyme was compared to the sequence of a homologous mesophilic enzyme from escherichia coli. the secondary structure of the thermophilic enzyme was predicted taking into account the patterns of hydropathy, chain flexibility and amphipathicity and the cd spectrum. from this analysis it turned out that indole-3-glyc ... | 1994 | 7947963 |
| effects of unusual polyamines on phenylalanyl-trna formation. | the effects of novel polyamines on aminoacyl-trna formation catalyzed by escherichia coli., sulfolobus acidocaldarius, and thermus thermophilus hb8 s-100 extracts were investigated. these effects were diverse and differed depending on the amino acid and the enzyme used. a quaternary polyamine, tetrakis(3-aminopropyl)ammonium, inhibited phenylalanyl-trna synthesis catalyzed by the t. thermophilus extract, but not inhibit the other aminoacyl-trna formations tested. the inhibition was observed in h ... | 1994 | 7961592 |
| purification of a dna topoisomerase ii from the hyperthermophilic archaeon sulfolobus shibatae. a thermostable enzyme with both bacterial and eucaryal features. | a type ii dna topoisomerase has been purified to homogeneity from the hyperthermophilic archaeon sulfolobus shibatae. the enzyme is composed of two subunits of 60 and 47 kda. it has a stokes radius of 69 a and has a sedimentation coefficient of 7.8 s which gives a calculated native molecular mass of approximately 230 kda, indicating a heterotetrameric structure. this enzyme is atp and mg2+ dependent and can relax both negatively and positively supercoiled dna, but presents no supercoiling activi ... | 1994 | 7961685 |
| in the thermophilic archaeon sulfolobus solfataricus a dna-binding protein is in vitro (adpribosyl)ated. | a small protein with high affinity for homologous dna was isolated from sulfolobus solfataricus homogenate by mineral acid extraction. it was purified using a two-step procedure including cm-cellulose and rp-hpl chromatographies. the protein was electrophoretically homogeneous, had a molecular weight of 7.147 kda and an amino acid composition with a high content of lysine and glutamic acid residues. the protein was able to protect dna against thermal denaturation and dnase i digestion in a dose- ... | 1995 | 7887965 |
| structure and transcription of the l11-l1-l10-l12 ribosomal protein gene operon from the extreme thermophilic archaeon sulfolobus acidocaldarius. | we have cloned and sequenced four ribosomal protein genes from the extreme thermophilic archaeon sulfolobus acidocaldarius p1. these genes code for proteins equivalent to l11, l1, l10 and l12 from escherichia coli. the genes for the sulfolobus l11, l1, l10 and l12 proteins are arranged in the same order as the equivalent genes in e. coli, i.e. l11-l1-l10-l12, and are transcribed as a single unit. sequences resembling the consensus sequence for archaeal promoters have been detected upstream of th ... | 1994 | 7966335 |
| occurrence of tertiary and quaternary branched polyamines in thermophilic archaebacteria. | polyamines of thermophilic archaebacteria were analysed by high-performance liquid chromatography and gas chromatography. thermoplasma acidophilum and thermoplasma volcanium ubiquitously contained spermidine and spermine. four species of sulfolobus, s. acidocaldarius, s. solfataricus, s. metallicus and s. shibatae, two species of acidianus, a. brierleyi and a. infernus, and metallosphaera sedula, contained norspermidine and norspermine in addition to spermidine and spermine, but quantitative dis ... | 1994 | 7968661 |
| the yeast type i topoisomerase top3 interacts with sgs1, a dna helicase homolog: a potential eukaryotic reverse gyrase. | we have previously shown that cells mutant for top3, a gene encoding a prokaryotic-like type i topoisomerase in saccharomyces cerevisiae, display a pleiotropic phenotype including slow growth and genome instability. we identified a mutation, sgs1 (slow growth suppressor), that suppresses both the growth defect and the increased genomic instability of top3 mutants. here we report the independent isolation of the sgs1 gene in a screen for proteins that interact with top3. dna sequence analysis rev ... | 1994 | 7969174 |
| sulfolobus acidocaldarius terminal oxidase. a kinetic investigation and its structural interpretation. | the thermoacidophilic archaebacterium sulfolobus acidocaldarius possesses a very unusual terminal oxidase. we report original kinetic experiments on membranes of this microorganism carried out by stopped flow, using time-resolved optical spectroscopy combined with singular value decomposition analysis. the reduced-oxidized kinetic difference spectrum of the sulfolobus membranes is characterized by three significant peaks in the visible region at 605, 586, and 560 nm. the 605-nm peak and part of ... | 1994 | 7983037 |
| the purified soxabcd quinol oxidase complex of sulfolobus acidocaldarius contains a novel haem. | a respiratory quinol oxidase complex that is encoded by the soxabcd operon has been purified from the thermoacidophilic archaeon sulfolobus acidocaldarius. the enzyme was solubilized with dodecyl maltoside and purified in the presence of this detergent and ethylene glycol. the complex is hydrodynamically homogeneous and contains at least five different polypeptides. in addition to the major subunits soxa, soxb and soxc, it has two small polypeptides. one of these is the translation product of a ... | 1994 | 7984110 |
| pyridine dinucleotide biosynthesis in archaebacteria: presence of nmn adenylyltransferase in sulfolobus solfataricus. | the enzyme nmn adenylyltransferase, leading to nad synthesis, has been observed for the first time in soluble extracts from the extreme acidothermophilic archaeon sulfolobus solfataricus. comparison of its molecular and kinetic properties with those of the enzyme isolated from prokaryotes and eukaryotes revealed significant differences, knowledge of which may contribute to the understanding of metabolic evolutionary mechanisms. the thermophilic enzyme shows a molecular mass of about 66,000 and a ... | 1994 | 7988679 |
| a spontaneous point mutation in the single 23s rrna gene of the thermophilic arachaeon sulfolobus acidocaldarius confers multiple drug resistance. | development of transformable vectors for thermophilic archaea requires the characterization of appropriate selectable marker genes. many antibiotic inhibitors of protein biosynthesis are known to bind to rrna; therefore, we screened 14 for their capacity to inhibit growth of the thermophilic archaeon sulfolobus acidocaldarius. carbomycin, celesticetin, chloramphenicol, puromycin, sparsomycin, tetracycline, and thiostrepton all inhibited growth by different degrees. spontaneous drug-resistant mut ... | 1994 | 8002603 |
| a few amino acid substitutions are responsible for the higher thermostability of a novel nad(+)-dependent bacillar alcohol dehydrogenase. | the gene adh-ht encoding a thermostable and thermophilic nad(+)-dependent alcohol dehydrogenase (adh) from the novel and more thermophilic bacillus stearothermophilus lld-r strain was cloned and its nucleotide sequence determined. the deduced protein sequence shows remarkable amino acid substitutions when compared to the sequence of the protein isolated from strain nca1503 and significant similarity with the highly thermostable adh from the thermoacidophilic archaebacterium sulfolobus solfataric ... | 1994 | 8020473 |
| functional and evolutionary implications of a [3fe-4s] cluster of the dicluster-type ferredoxin from the thermoacidophilic archaeon, sulfolobus sp. strain 7. | the dicluster-type ferredoxin is a key electron carrier in the cytoplasm of the aerobic and thermoacidophilic archaeon, sulfolobus sp. strain 7, and contains 1 aspartate and 7 cysteine residues as possible ligands to two fes clusters. the optical, electron paramagnetic resonance (epr), and cyclic voltammetric studies suggest the presence of one each of [3fe-4s]1+,0 (-280 mv) and [4fe-4s]2+,1+ (-530 mv) clusters in the purified sulfolobus ferredoxin, and the lower potential [4fe-4s] center was sc ... | 1994 | 7961925 |
| evidence for a two proton dependent redox equilibrium in an archaeal rieske iron-sulfur cluster. | the recently detected rieske iron-sulfur center in the membrane of the thermoacidophilic archaeon sulfolobus acidocaldarius (anemüller et al., 1993, febs lett. 318, 61-64) was further characterized by epr spectroscopy, coupled to redox-potentiometry and functional studies. the reduction potential is ph-dependent above ph 6, revealing the influence of two ionization equilibria in the oxidized form, with pkaox-values of 6.2 and 8.5. above ph 9, the slope of the curve is--120 mv/ph-unit. a partiall ... | 1994 | 8037720 |
| unusual thermal stability of liposomes made from bipolar tetraether lipids. | the thermal stability of liposomes made from polar lipids of sulfolobus acidocaldarius, a thermoacidophilic archaebacterium, was studied. while two analogous nonarchaebacterial liposomes examined were unstable above 50 degrees c, liposomes made of the native tetraether lipids showed stability up to at least 80 degrees c. incubation of the archaebacterial liposomes at 100 degrees c, under slight pressure, caused an approximate 15% leakage after 60 minutes. boiling, however, destroys the integrity ... | 1994 | 8048936 |
| archaebacterial elongation factor 1 alpha carries the catalytic site for gtp hydrolysis. | the elongation factor 1 alpha from the archaebacterium sulfolobus solfataricus (aef-1 alpha) possesses an intrinsic gtpase activity that is triggered by nacl up to 5.2 m and requires the presence of at least 1 mm mgcl2 or mncl2. chloride salts of other monovalent cations are inefficient, whereas other sodium salts are much less efficient or not efficient at all as compared with nacl. this aef-1 alpha gtpase (gtpasena) reaches a maximum in a broad ph range and is not affected by other nucleoside ... | 1994 | 8051132 |
| stability and proton-permeability of liposomes composed of archaeal tetraether lipids. | liposomes composed of tetraether lipids originating from the thermoacidophilic archaeon sulfolobus acidocaldarius were analyzed for their stability and proton permeability from 20 degrees c up to 80 degrees c. at room temperature, these liposomes are considerably more stable and have a much lower proton permeability than liposomes composed of diester lipids originating from the mesophilic bacterium escherichia coli or the thermophilic bacterium bacillus stearothermophilus. with increasing temper ... | 1994 | 8054346 |
| the amino acid sequence of glutamate dehydrogenase from pyrococcus furiosus, a hyperthermophilic archaebacterium. | the complete amino acid sequence of glutamate dehydrogenase from the archaebacterium pyrococcus furiosus has been determined. the sequence was reconstructed by automated sequence analysis of peptides obtained after cleavage with cyanogen bromide, asp-n endoproteinase, trypsin, or pepsin. the enzyme subunit is composed of 420 amino acid residues yielding a molecular mass of 47,122 d. in the recently determined primary structure of glutamate dehydrogenase from another thermophilic archaebacterium, ... | 1994 | 8060497 |
| novel prenylated hemes as cofactors of cytochrome oxidases. archaea have modified hemes a and o. | a series of novel hemes with modifications of the isoprenyl side chain has been detected in archaea. heme a(s) was isolated from cytochrome oxidases of the thermoacidophilic archaeon, sulfolobus acidocaldarius. heme a(s) has the same spectroscopic features as heme a but has a hydroxyethylgeranylgeranyl side chain instead of the hydroxyethylfarnesyl group. this variant is also present in other archaeal oxidases as well as in the cytochrome oxidases of a thermophilic eubacterium. other archaea (th ... | 1994 | 8063781 |
| calcium-induced interaction and fusion of archaeobacterial lipid vesicles: a fluorescence study. | the lipids extracted from the membrane of the thermophilic archaeobacterium sulfolobus solfataricus have an unusual bipolar structure. each molecule is formed by two isoprenoid chains (with up to four cyclopentane groups per chain) ether-linked at both ends to glycerol or nonitol groups. these groups can be variably substituted, mainly with complex sugars. fluorescence resonance energy transfer, aqueous contents mixing and calcein release assays were employed to assess whether bipolar lipid vesi ... | 1994 | 8075132 |
| a second terminal oxidase in sulfolobus acidocaldarius. | we previously found that the soxabcd operon encodes a quinol oxidase complex in sulfolobus acidocaldarius and this enzyme was purified and characterized. in this study, we have used a cloning procedure based on the conservation of oxidase sequences and the polymerase chain reaction to isolate a new gene (soxm) encoding a subunit of another terminal oxidase. this terminal oxidase is a fusion between two central components of cytochrome oxidases, subunits i and iii. soxm forms a transcriptional un ... | 1994 | 8076636 |
| dna unknotting activity (dna topoisomerase ii) isolated from a thermophilic archaebacterium sulfolobus is inhibited by novobiocin. partial purification, identification of the two subunits and characteristics of the enzyme. | dna topoisomerases ii are enzymes which have been purified from a lot of organisms and have been found to be involved in segregation of chromosomes. the following article reports the analysis of a partially purified dna topoisomerase ii from sulfolobus (strain b12) a thermophilic archaebacterium which grows at 80 degrees c. the enzyme is composed by two subunits: the a subunit with a molecular mass of 85,000 da which contains the nicking-closing activity and the b subunit with a molecular mass o ... | 1994 | 8086447 |
| crystallization of a hyperthermophilic archaeal elongation factor 1 alpha. | intact and fully active elongation factor aef-1 alpha from the hyperthermophilic archaeon sulfolobus solfataricus has been crystallized as a complex with gdp. crystals were stable at temperatures below 8 degrees c and showed significant diffraction beyond 3.0 a. the orthorhombic lattice parameters were a = 62.9 a, b = 81.3 a, c = 115.6 a with one molecule per asymmetric unit. | 1994 | 8089838 |
| stoichiometry of sulfolobus ribosomal protein l12e in 50s subunits determined by quantification of immunoblots. | a monoclonal antibody reactive with sulfolobus solfataricus acidic ribosomal protein ssol12e was prepared and employed to determine the stoichiometry of this protein in 50s ribosomal subunits by quantification of chloronaphthol-stained protein bands from immunoblots. approximately four copies of ssol12e were detected per 50s ribosome. this finding extends previous studies demonstrating the involvement of this protein in a multimeric protein complex in the ribosomal factor binding domain of sulfo ... | 1994 | 8093032 |
| cloning and sequencing of the gene encoding glutamine synthetase i from the archaeum pyrococcus woesei: anomalous phylogenies inferred from analysis of archaeal and bacterial glutamine synthetase i sequences. | the gene glna encoding glutamine synthetase i (gsi) from the archaeum pyrococcus woesei was cloned and sequenced with the sulfolobus solfataricus glna gene as the probe. an operon reading frame of 448 amino acids was identified within a dna segment of 1,528 bp. the encoded protein was 49% identical with the gsi of methanococcus voltae and exhibited conserved regions characteristic of the gsi family. the p. woesei gsi was aligned with available homologs from other archaea (s. solfataricus, m. vol ... | 1993 | 8098326 |
| purification and characterization of reverse gyrase from sulfolobus shibatae. its proteolytic product appears as an atp-independent topoisomerase. | sulfolobus shibatae b12 is a thermophilic archaebacterium that contains an inducible virus named ssv1. the viral dna has been shown to be positively supercoiled before encapsidation. we have previously purified an archaebacterial dna topoisomerase from sulfolobus acidocaldarius dsm 639, reverse gyrase, likely responsible for this positive supercoiling reaction. in order to study an homogeneous system containing both reverse gyrase and one of its preferential substrate, ssv1 dna, we have purified ... | 1994 | 8106509 |
| molecular cloning, nucleotide sequence and expression of a sulfolobus solfataricus gene encoding a class ii fumarase. | | 1994 | 8119400 |
| pcr-mediated cloning and sequencing of the gene encoding glutamate dehydrogenase from the archaeon sulfolobus shibatae: identification of putative amino-acid signatures for extremophilic adaptation. | highly degenerate oligodeoxyribonucleotides (oligos) were used to pcr amplify the most conserved region of the glutamate dehydrogenase (gdh)-encoding gene from the extreme thermophilic archaeon, sulfolobus shibatae. the amplified fragment was cloned and sequenced, and then used as a homologous probe to clone a genomic restriction fragment containing the near-complete gdha gene. the deduced amino acid (aa) sequence shows a very high degree of similarity with the aa sequence previously determined ... | 1994 | 8125334 |
| a subunit of an archaeal dna-dependent rna polymerase contains the s1 motif. | | 1994 | 8127719 |
| evidence that an archaeal alpha-like dna polymerase has a modular organization of its associated catalytic activities. | in this study we report on the evidence that an alpha-like dna polymerase purified from the thermoacidophilic archaeon sulfolobus solfataricus has a modular organization of its associated catalytic activities (polymerase and 3'-5' exonuclease). this enzyme, a monomer of about 100 kda whose complete primary structure is available, has a protease hypersensitive site that is likely to be cleaved by the action of endogenous proteases during the purification procedure. as a consequence of that, two p ... | 1994 | 8132506 |
| the nucleotide sequence of the gene coding for the elongation factor 1 alpha in sulfolobus solfataricus. homology of the product with related proteins. | the cloning and sequencing of the gene coding for the archaebacterial elongation factor 1 alpha (aef-1 alpha) was performed by screening a sulfolobus solfataricus genomic library using a probe constructed from the eptapeptide knmitga that is conserved in all the ef-1 alpha/ef-tu known so far. the isolated recombinant phage contained the part of the aef-1 alpha gene from amino acids 1 to 171. the other part (amino acids 162-435) was obtained through the amplification of the s. solfataricus dna by ... | 1994 | 8148382 |
| sequencing and characterization of the ntp gene cluster for vacuolar-type na(+)-translocating atpase of enterococcus hirae. | we have previously reported the dna and amino acid sequences for the three genes (ntpa, ntpb, and ntpk) encoding the a, b, and k (proteolipid) subunits, respectively, of na(+)-translocating atpase of a eubacterium enterococcus hirae (kakinuma, y., kakinuma, s., takase, k., konishi, k., igarashi, k., and yamato, i. (1993) biochem. biophys. res. commun. 195, 1063-1069). in this paper we report the entire nucleotide sequence of the ntp gene cluster coding for this multisubunit enzyme. the cluster c ... | 1994 | 8157629 |
| the sequence, and its evolutionary implications, of a thermococcus celer protein associated with transcription. | through random search, a gene from thermococcus celer has been identified and sequenced that appears to encode a transcription-associated protein (110 amino acid residues). the sequence has clear homology to approximately the last half of an open reading frame reported previously for sulfolobus acidocaldarius [langer, d. & zillig, w. (1993) nucleic acids res. 21, 2251]. the protein translations of these two archaeal genes in turn are homologs of a small subunit found in eukaryotic rna polymerase ... | 1994 | 8171001 |
| bacterial catalytic processes for transformation of metals. | microorganisms actively participate in the transformation of metals and metalloids by various processes including adsorption, absorption, alkylation, oxidation and reduction reactions. bacteria of the genera thiobacillus and sulfolobus have a chemolithotrophic mode of metabolism and catalyze various metal transformations. these bacteria are primarily involved in oxidation-reduction reactions of metals. metal sulfides can be either directly oxidized by the microbes or oxidized by ferric iron, an ... | 1993 | 8181951 |
| archaebacterial ether-linked lipid biosynthetic gene. expression cloning, sequencing, and characterization of geranylgeranyl-diphosphate synthase. | archaebacterial sulfolobus acidocaldarius geranylgeranyl-diphosphate (ggpp) synthase (ec 2.5.1.29) catalyzes consecutive condensations of isopentenyl diphosphate with allylic diphosphates to produce ggpp which is the important precursor of archaebacterial ether-linked lipids. we developed an expression screening method for cloning the ggpp synthase gene, which utilizes the carotenoid biosynthesis genes of erwinia uredovora to visualize a clone expressing ggpp synthase, and then screened a genomi ... | 1994 | 8182085 |
| an insertion element of the extremely thermophilic archaeon sulfolobus solfataricus transposes into the endogenous beta-galactosidase gene. | three phenotypically stable mutants of the extremely thermophilic archaeon sulfolobus solfataricus have been isolated by screening for beta-galactosidase negative colonies on plates with x-gal (5-bromo-4-chloro-3-indolyl-beta-d- galactopyranoside). from one of these mutants an insertion element, designated isc1217, was isolated and characterized. sequence analysis of isc1217 and of the regions adjacent to the insertion site in the beta-galactosidase gene revealed features typical of a transposab ... | 1994 | 8190076 |
| the molecular chaperone tf55. assessment of symmetry. | tf55-like factor from sulfolobus solfataricus was purified to homogeneity and analyzed by electron microscopy and image analysis to determine the symmetries of these particles. three different procedures were used to analyze the electron micrographs: (1) fuzzy-set based classification of the particles according to their rotational power spectra; (2) multivariate statistical analysis based on singular value decomposition; (3) circular harmonic analysis. averages obtained from the three methods sh ... | 1994 | 7907992 |
| structural features responsible for kinetic thermal stability of a carboxypeptidase from the archaebacterium sulfolobus solfataricus. | investigations were performed on the structural features responsible for kinetic thermal stability of a thermostable carboxypeptidase from the thermoacidophilic archaebacterium sulfolobus solfataricus which had been purified previously and identified as a zinc metalloprotease [colombo, d'auria, fusi, zecca, raia and tortora (1992) eur. j. biochem. 206, 349-357]. removal of zn2+ by dialysis led to reversible activity loss, which was promptly restored by addition of 80 microm zncl2 to the assay mi ... | 1993 | 8240298 |
| biological activities of cell envelope fragments of the archaeobacterium sulfolobus solfataricus: lethal toxicity, local hypersensitivity, pyrogenicity and spleen lymphocyte mitogenicity. | the sensitizing effect and the local and general toxicity related to membrane components of the archaeobacterium sulfolobus solfataricus was studied. cell envelope fragments were biologically active but this activity was lost upon separation of the lipid and protein components. the envelope fragments exerted lethal effects on mice sensitized with d-galactosamine that were prevented by pretreatment with anti-tnf-alpha serum. this lethal activity occurred in both lps-responder (balb/cbyj) and lps- ... | 1993 | 8245842 |
| nucleotide sequence and molecular evolution of the gene coding for glyceraldehyde-3-phosphate dehydrogenase in the thermoacidophilic archaebacterium sulfolobus solfataricus. | a sulfolobus solfataricus genomic library cloned in the embl3 phage was screened using as probes synthetic oligonucleotides designed from the known amino acid sequence of a peptide obtained from the purified glyceraldehyde-3-phosphate dehydrogenase (agapd) protein. the screening led to the isolation of six recombinant phages (lambda g1-lambda g6) and one of them (lambda g4) contained the entire gapd gene. the deduced amino acid sequence accounts for a protein made of 341 amino acids and the init ... | 1993 | 8259927 |
| identification, cloning, and expression of the gene for adenylate kinase from the thermoacidophilic archaebacterium sulfolobus acidocaldarius. | an adenylate kinase gene from a member of the archaebacterial kingdom, the thermoacidophilic archaebacterium (archaeon) sulfolobus acidocaldarius, has been cloned and sequenced for the first time. two degenerate oligonucleotide probes, based on the n-terminal amino acid sequence information, led to the amplification of a gene-specific dna fragment, used to screen subgenomic libraries. comparing the dna-derived amino acid sequence of total 194 residues with those of known procaryotic and eucaryot ... | 1993 | 8274029 |
| molecular cloning, nucleotide sequence and expression of a sulfolobus solfataricus gene encoding a class ii fumarase. | fumarase catalyzes the interconversion of l-malate and fumarate. a sulfolobus solfataricus fumarase gene (fumc) was cloned and sequenced. typical archaebacterial regulatory sites were identified in the region flanking the fumc open reading frame. the fumc gene encodes a protein of 438 amino acids (47,899 da) which shows several significant similarities with class ii fumarases from both eubacterial and eukariotic sources as well as with aspartases. s. solfataricus fumarase expressed in escherichi ... | 1994 | 8276121 |
| an ecto-enzyme from sulfolobus acidocaldarius strain 7 which catalyzes hydrolysis of inorganic pyrophosphate, atp, and adp: purification and characterization. | membranes of sulfolobus acidocaldarius, a thermoacidophilic archaebacterium, show novel enzymatic activities to hydrolyze ppi, atp, and adp at an optimal ph of 3, equal to the growth optimum. the activity increased by about 2-fold on addition of ppi and/or pi to the growth medium, when yeast extract and casamino acids were removed. the enzyme which hydrolyzes ppi at ph 3 was solubilized and purified by successive chromatographies. the final preparation showed a 26 kda single band on sds-page, an ... | 1993 | 8282721 |
| strain identification and 5s rrna gene characterization of the hyperthermophilic archaebacterium sulfolobus acidocaldarius. | a commonly used laboratory sulfolobus strain has been unambiguously identified as sulfolobus acidocaldarius dsm639. the 5s rrna gene from this strain was cloned and sequenced. it differs at 17 of 124 positions from the identical 5s rrna sequences from sulfolobus solfataricus and a strain apparently misidentified as s. acidocaldarius. analysis of the transcripts from the 5s rrna gene failed to identify any precursor extending a significant distance beyond the 5' or 3' boundary of the 5s rrna-codi ... | 1994 | 8288546 |
| sav, an archaebacterial gene with extensive homology to a family of highly conserved eukaryotic atpases. | nucleotide sequencing of a region of the hyperthermophilic archaebacterium sulfolobus acidocaldarius allowed us to identify an open reading frame of 780 amino acids strikingly similar to a family of eukaryotic atpases, involved in a variety of biological functions. sequence analysis of the predicted polypeptide revealed 63 to 66% similarity with s. cerevisiae cdc 48p and its related genes in amphibians (p97atpase) and mammals (valosin containing protein, vcp), all possibly involved in the regula ... | 1994 | 8289263 |
| cloning and sequencing of the gene encoding thermostable elongation factor 2 in sulfolobus solfataricus. | the gene (aef-2) coding for the translation elongation factor 2 (aef-2) in the thermoacidophilic archaebacterium, sulfolobus solfataricus, has been cloned and sequenced. the deduced primary structure of aef-2 is composed of 735 amino acids (aa), excluding the met start residue. there are no cys residues and the calculated m(r) is 81,699. in the coding region of aef-2, the high a + t content greatly influences the codon usage. from the alignment of the primary structure of aef-2 with that of the ... | 1993 | 8294039 |
| comparative studies on thermophilicity and thermostability of aspartate aminotransferases. | aspartate aminotransferase from sulfolobus solfataricus (aspatss) is an extremely thermophilic and thermostable enzyme. in order to investigate the structural features which underlie thermophilicity and thermostability, two isoforms of aspatss differing by a single amino acid residue were compared. the first isoform is the naturally occurring enzyme, whereas the second is a genetically engineered mutant. thermophilicity, short-term and long-term thermostability of the isoenzymes were independent ... | 1993 | 8297515 |
| asymmetric black membranes formed by one monolayer of bipolar lipids at the air/water interface. | in this work a new technique is presented for the formation of black lipid membranes from a single monolayer of bipolar lipids at the air/water interface. the lipid, extracted from the thermophilic archaeobacterium sulfolobus solfataricus, is characterized by two different polar heads. the membrane is formed with a technique similar to that introduced by montal and mueller; however, the lipid is spread only on one side of the teflon partition. conductance in the presence of valinomycin, voltage- ... | 1994 | 8305465 |
| generation of proton-motive force by an archaeal terminal quinol oxidase from sulfolobus acidocaldarius. | the terminal quinol oxidase of the cytochrome aa3 type was isolated from the extreme thermoacidophilic archaeon sulfolobus acidocaldarius. in micellar solution, the enzyme oxidized various quinols and exerted the highest activity with the physiological substrate caldariella quinol. the enzyme was functionally reconstituted into monolayer liposomes composed of archaeal tetraether lipids also derived from s. acidocaldarius. with the electron donor system ascorbate and n,n,n',n'-tetramethyl-p-pheny ... | 1994 | 7925423 |
| s-adenosylhomocysteine hydrolase from the thermophilic archaeon sulfolobus solfataricus: purification, physico-chemical and immunological properties. | s-adenosylhomocysteine hydrolase from sulfolobus solfataricus, a thermoacidophilic archaeon optimally growing at 87 degrees c, has been purified to homogeneity. the specific activity of the homogeneous enzyme is 161 nmol of s-adenosylhomocysteine formed per min per mg of protein, and the overall yield, by immunoaffinity purification, is 51%. the enzyme has a molecular mass of 190 kda, is composed of four identical subunits (subunit mass 47 kda), and contains four molecules of tightly-bound nad+ ... | 1993 | 8329449 |
| aspartate aminotransferase from a thermophilic formate-utilizing methanogen, methanobacterium thermoformicicum strain sf-4: relation to serine and phosphoserine aminotransferases, but not to the aspartate aminotransferase family. | the primary structure of the aspartate aminotransferase (aspat) of an archaebacterium, methanobacterium thermoformicicum strain sf-4, has been determined by cloning and sequencing of the gene for the enzyme. the gene had a consensus promoter and a ribosome binding sequence of methanogens in the 5' untranslated region, followed by an open reading frame starting with atg and terminating with tga. the deduced amino acid sequence was identical with the partial amino acid sequences of the enzyme incl ... | 1994 | 8206881 |
| nucleotide sequence, transcription and phylogeny of the gene encoding the superoxide dismutase of sulfolobus acidocaldarius. | the gene encoding the superoxide dismutase (sod) of the thermophilic archaeon sulfolobus acidocaldarius has been isolated and sequenced. both the start site and the termination sites of the corresponding transcript were mapped. the deduced amino acid sequence of the protein is very similar to the sequence of manganese- or iron-containing sods. phylogenetic sequence analysis corroborated the monophyletic nature of the archaeal domain. | 1993 | 8334170 |
| primary structure of the elongation factor 1 alpha in sulfolobus solfataricus. | | 1993 | 8369039 |
| post-translational modifications in aspartate aminotransferase from sulfolobus solfataricus. detection of n-epsilon-methyllysines by mass spectrometry. | advanced mass spectrometric procedures have been extensively used to provide an accurate structural characterization of aspartate aminotransferase from sulfolobus solfataricus. the amino acid sequence of this enzyme had previously been deduced from the dna sequence. the accurate molecular mass of the protein, determined using electrospray mass spectrometry, demonstrated that the amino acid sequence deduced was correct and ruled out the possible presence of large covalent modifications which had ... | 1994 | 8026489 |
| evolution of glutamate dehydrogenase genes: evidence for two paralogous protein families and unusual branching patterns of the archaebacteria in the universal tree of life. | the existence of two families of genes coding for hexameric glutamate dehydrogenases has been deduced from the alignment of 21 primary sequences and the determination of the percentages of similarity between each pair of proteins. each family could also be characterized by specific motifs. one family (family i) was composed of gdh genes from six eubacteria and six lower eukaryotes (the primitive protozoan giardia lamblia, the green alga chlorella sorokiniana, and several fungi and yeasts). the o ... | 1993 | 8315654 |
| identification of a serine/threonine-specific protein phosphatase from the archaebacterium sulfolobus solfataricus. | we have observed that soluble extracts from the extreme acidothermophilic archaebacterium sulfolobus solfataricus contained protein phosphatase activity that was greatly stimulated by the divalent metal ions mn2+, mg2+, ni2+, or co2+. this activity apparently arose from a single enzyme since (a) stimulation by these divalent metal ions was not additive and (b) protein phosphatase activity eluted as a single peak from both a de52 ion-exchange column and a sephadex g-100 gel filtration column. its ... | 1993 | 8384214 |
| reverse gyrase: a helicase-like domain and a type i topoisomerase in the same polypeptide. | reverse gyrase is a type i dna topoisomerase able to positively supercoil dna and is found in thermophilic archaebacteria and eubacteria. the gene coding for this protein was cloned from sulfolobus acidocaldarius dsm 639. analysis of the 1247-amino acid sequence and comparison of it with available sequence data suggest that reverse gyrase is constituted of two distinct domains: (i) a c-terminal domain of approximately 630 amino acids clearly related to eubacterial topoisomerase i (escherichia co ... | 1993 | 8389456 |
| a gene encoding the 16-kda proteolipid subunit of enterococcus hirae na(+)-atpase complex. | by further sequencing the cloned genome dna of enterococcus hirae that retains the genes encoding two major subunits of na(+)-transport atpase (takase, k., yamato, i., and kakinuma, y. (1993) j. biol. chem. 268, 11610-11616), we found a gene, ntpk, encoding a very hydrophobic protein of 156 amino acids (mr = 16,036). the amino acid sequence deduced from the ntpk gene matched with the partial sequence of a 16-kda protein purified as the proteolipid component of na(+)-atpase. the amino acid sequen ... | 1993 | 8373385 |
| a protein-serine phosphatase from the halophilic archaeon haloferax volcanii. | we have detected a protein phosphatase activity in soluble extracts from the halophilic archaeon haloferax volcanii. this activity was markedly stimulated by the divalent metal ions mn2+ and cd2+. it dephosphorylated phosphoseryl residues in casein, mixed histones, and phosphorylase a, but not phosphotyrosyl residues in reduced, carboxyamidomethylated and maleylated lysozyme. this protein phosphatase activity was inhibited by naf, zn2+, vanadate, molybdate, inorganic phosphate, inorganic pyropho ... | 1993 | 8394705 |
| unusual heme structure in cytochrome aa3 from sulfolobus acidocaldarius: a resonance raman investigation. | well-resolved soret-excited resonance raman spectra have been obtained in the heme marker band region (1440-1700 cm-1) for the fully oxidized and fully reduced forms of the terminal oxidase aa3 from sulfolobus acidocaldarius. the results demonstrate that in both heme groups the structural properties depart from those of other aa3 oxidases. in the fully oxidized form, the formyl stretching vibration of heme a is observed at 1656 cm-1, approximately 7 cm-1 higher in frequency than found for beef h ... | 1993 | 8399237 |
| glutamate dehydrogenase from the thermoacidophilic archaebacterium sulfolobus solfataricus: studies on thermal and guanidine-dependent inactivation. | the hexameric nad(p)-dependent glutamate dehydrogenase isolated from the thermoacidophilic archaebacterium sulfolobus solfataricus shows a remarkable thermal stability which is strictly dependent on protein concentration (half-life at 95 degrees c is 0.25 h and 0.5 h at 0.4 and 0.8 mg/ml, respectively). temperature-dependent inactivation of the enzyme is apparently irreversible; this process is accompanied by a progressive increase in hydrophobic surface area which leads to protein precipitation ... | 1993 | 8399381 |
| tryptophan biosynthesis genes trpegc in the thermoacidophilic archaebacterium sulfolobus solfataricus. | a dna fragment containing the trpegc gene cluster was isolated from the thermoacidophilic archaebacterium sulfolobus solfataricus. the products of trpe, trpg, and trpc from s. solfataricus were compared to the homologous products from a eukaryote, a eubacterium, and two archaebacteria, namely, a methanogen and an extreme halophile. they appeared to be equally related to the proteins from escherichia coli and saccharomyces cerevisiae, the percentages of conserved amino acids being roughly the sam ... | 1993 | 8416906 |
| purification and characterization of kumamolysin, a novel thermostable pepstatin-insensitive carboxyl proteinase from bacillus novosp. mn-32. | we have found a novel type of thermostable, pepstatin-insensitive carboxyl proteinase in the culture filtrate of bacillus novosp. mn-32. the carboxyl proteinase, which was named kumamolysin, was purified about 8,300-fold by column chromatography including deae-sepharose cl-6b, sephadex g-100, and tskgel deae-5pw. the purified kumamolysin gave a single band corresponding to 41 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular mass of kumamolysin was estimated to be 4 ... | 1993 | 8416942 |
| 5s rrna modification in the hyperthermophilic archaea sulfolobus solfataricus and pyrodictium occultum. | the 5s rrnas from sulfolobus solfataricus and pyrodictium occultum were digested to nucleosides and analyzed using directly-combined hplc/mass spectrometry. p. occultum 5s rrna contains two modified nucleoside species, n4-acetylcytidine (ac4c) and n4-acetyl-2'-o-methylcytidine (ac4cm). oligonucleotides were generated from p. occultum 5s rrna by rnase t1 hydrolysis, and their molecular weights were determined using electrospray mass spectrometry and compared with those predicted from the p. occul ... | 1993 | 8422966 |
| ribonucleases from the extreme thermophilic archaebacterium s. solfataricus. | a purification procedure consisting of deae-sephacel chromatography, heparin-sepharose cl-6b chromatography and mono-s chromatography led to the isolation of three proteins endowed with rnase activity from the thermoacidophilic archaebacterium sulfolobus solfataricus. they were referred to as p1, p2 and p3, according to their elution order from the mono-s column. complete amino acid sequence of p2 and partial sequence of p3 displayed high sequence similarity to the 7-kda dna-binding proteins pre ... | 1993 | 8425540 |
| evidence for a rieske-type fes center in the thermoacidophilic archaebacterium sulfolobus acidocaldarius. | a high-potential iron-sulfur cluster with characteristics similar to a rieske-type center was detected in the plasma membrane of sulfolobus acidocaldarius by epr spectroscopy. in the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potential at ph 7.4 was determined to be +325 +/- 10 mv. the the archaebacterial cluster exhibits some unique properties, in comparison to eubacterial and eukaryotic rieske-type centers. f ... | 1993 | 8382170 |
| crystallization and preliminary x-ray analysis of an nad(+)-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium sulfolobus solfataricus. | an nad(+)-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium sulfolobus solfataricus has been crystallized in the holo-enzyme and apo-enzyme forms. crystals of the holo-enzyme grow from 2-methyl-2,4-pentanediol at ph 8.4 with the addition of nadh and at ph 7.0 with the addition of nadh and dimethyl sulphoxide. crystals of the apo-enzyme grow at ph 6.3 from a mixture of polyethylene glycol 4000 and propan-2-ol. the holo-enzyme crystallizes in c2 with a dimer in the asym ... | 1993 | 8433371 |
| sulfolobus acidocaldarius synthesizes ump via a standard de novo pathway: results of biochemical-genetic study. | a genetic approach was used to establish the route of ump biosynthesis in sulfolobus acidocaldarius, a member of the hyperthermophilic division (the crenarchaeota) of the archaea domain. pyrimidine auxotrophs of s. acidocaldarius dg6 were isolated by direct selection and by brute-force methods. enzymatic assay of extracts from wild-type s. acidocaldarius, from pyrimidine auxotrophs, and from phenotypic revertants demonstrated that s. acidocaldarius synthesizes ump via orotate in six enzymatic st ... | 1993 | 8444810 |
| resistance of archaebacterial aef-1 alpha.gdp against denaturation by heat and urea. | the elongation factor aef-1 alpha, isolated as aef-1 alpha.gdp from the thermoacidophilic archaebacterium sulfolobus solfataricus, exchanges gdp for [3h]gdp at a rate which reaches a maximum at 95 degrees c. the rate constants at different temperatures of the heat inactivation of aef-1 alpha.gdp are considerably lower compared to those referred to escherichia coli ef-tu.gdp. the tm values determined for both aef-1 alpha.gdp and ef-tu.gdp are 97 and 53 degrees c, respectively. the addition of gdp ... | 1993 | 8448192 |
| properties of the purified elongation factor 2 in the thermoacidophilic archaebacterium sulfolobus solfataricus. | the elongation factor 2 (aef-2) has been purified to homogeneity from the extreme thermoacidophilic archaebacterium sulfolobus solfataricus. it is the only target protein which is adp-ribosylated by diphtheria toxin in presence of nad and this modification abolishes its property to support poly(phe) synthesis in vitro. the factor is constituted by a single polypeptide chain with a relative molecular mass of 78,000 and an isoelectric point of 5.9. aef-2 is resistant to heat denaturation as shown ... | 1993 | 8468156 |
| ssv1-encoded site-specific recombination system in sulfolobus shibatae. | we present evidence for the existence of a conservative site-specific recombination system in archaea by demonstrating integrative recombination of sulfolobus shibatae virus ssv1 dna with the host chromosome, catalysed by the ssv1-encoded integrase in vitro. the putative int gene of ssv1 was expressed in escherichia coli yielding a protein of about 39 kda. this protein alone efficiently recombined linear dna substrates containing chromosomal (atta) and viral (attp) attachment sites; recombinatio ... | 1993 | 8483447 |
| exo-glucosidase activity and substrate specificity of the beta-glycosidase isolated from the extreme thermophile sulfolobus solfataricus. | the enzyme with beta-galactosidase activity from sulfolobus solfataricus strain mt-4, like other enzymes of this type isolated from thermophilic sources, has broad specificity for beta-d-gluco-, fuco- and galacto-sides. the beta-galactosidase activity was purified by a new procedure that improved yields (44%) and final specific activity (182 units mg-1 at 75 degrees c using chromogenic beta-d-galactoside as substrate). the enzyme hydrolysed a large number of beta-linked glycoside dimers and olig ... | 1993 | 8484908 |
| archaebacterial adenylate kinase from the thermoacidophile sulfolobus acidocaldarius: purification, characterization, and partial sequence. | for the first time an adenylate kinase of archaebacterial origin has been purified to homogeneity. the enzyme from the extreme thermoacidophile sulfolobus acidocaldarius (dsm 639) has been found to consist of a 23- to 24-kda polypeptide likely to form a dimer under in vitro conditions. its temperature optimum is about 90 degrees c; the ph optimum is 5.3-6.0, depending on the observed direction of the reaction. the km values for atp, amp, and adp are almost equal (0.6-0.7 mm). the enzyme is absol ... | 1993 | 8489243 |
| putative tfiis gene of sulfolobus acidocaldarius encoding an archaeal transcription elongation factor is situated directly downstream of the gene for a small subunit of dna-dependent rna polymerase. | | 1993 | 8502569 |
| cloning and sequencing of the genes coding for the a and b subunits of vacuolar-type na(+)-atpase from enterococcus hirae. coexistence of vacuolar- and f0f1-type atpases in one bacterial cell. | the eubacterium enterococcus hirae atcc 9790 possesses a h(+)-translocating atpase, and the deduced amino acid sequences of the genes coding for this enzyme have indicated that it is a typical f0f1-type atpase (shibata, c., ehara, t., tomura, k., igarashi, k., and kobayashi, h. (1992) j. bacteriol. 174, 6117-6124). we cloned the ntpa and ntpb genes coding for the a and b subunits, respectively, of na(+)-translocating atpase from the same bacterium, and the full amino acid sequences of the two su ... | 1993 | 8505293 |
| nucleotide sequence of the gene for a 74 kda dna polymerase from the archaeon sulfolobus solfataricus. | | 1993 | 8332474 |
| the phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon sulfolobus solfataricus overlap by 8-bp. isolation, sequencing of the genes and expression in escherichia coli. | the overlapping genes encoding phosphoglycerate kinase (pgk) and glyceraldehyde-3-phosphate dehydrogenase (grap-dh) from the hyperthermophilic archaeon sulfolobus solfataricus have been cloned and sequenced. pcr primers based on highly conserved regions of different pgk sequences were used to isolate an internal region of the pgk gene. this was then used to screen a genomic library to isolate the full length pgk gene. a 2.5-kb bglii fragment of s. solfataricus dna contained both the pgk gene and ... | 1995 | 8521845 |
| identification of the iron-sulfur clusters in a ferredoxin from the archaeon sulfolobus acidocaldarius. evidence for a reduced [3fe-4s] cluster with ph-dependent electronic properties. | a ferredoxin isolated from the archaeon sulfolobus acidocaldarius strain dsm 639 has been shown to contain one [3fe-4s]1 + 10 cluster with a reduction potential of -275 mv and one [4fe-4s]2+/1+ cluster with a reduction potential of -529 mv at ph 6.4, in the temperature range 0-50 degrees c. the monomer molecular mass was confirmed to be 10907.5 +/- 1.0 da by electrospray mass spectrometry, as calculated from the published amino acid sequence [minami, y. wakabayashi. s., wada, k., matsubara, h., ... | 1995 | 8521862 |
| expression of an archaeal chaperonin in e. coli: formation of homo- (alpha, beta) and hetero-oligomeric (alpha+beta) thermosome complexes. | co-expression of the two genes encoding the alpha- and beta-subunit of the thermoplasma acidophilum thermosome in escherichia coli yielded fully assembled hetero-oligomeric complexes (alpha+beta). surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the bacterial cytoplasm. on electron micrographs these complexes were indistinguishable from each other and from the native thermosome. the recombinant alpha-complex as well as the nat ... | 1995 | 8521970 |
| resolution of the aerobic respiratory system of the thermoacidophilic archaeon, sulfolobus sp. strain 7. i. the archaeal terminal oxidase supercomplex is a functional fusion of respiratory complexes iii and iv with no c-type cytochromes. | the aerobic respiratory system of the thermoacidophilic archaeon, sulfolobus sp. strain 7, is unusual in that it consists of only a- and b-type cytochromes but no c-type cytochromes. in previous studies, a novel cytochrome oxidase a583-aa3 subcomplex has been purified, which showed a ferrocytochrome c oxidase but no caldariellaquinol oxidase activity (wakagi, t., yamauchi, t., oshima, t., müller, m., azzi, a., and sone, n. (1989) biochem. biophys. res. commun. 165, 1110-1114). we show here that ... | 1995 | 8537342 |
| resolution of the aerobic respiratory system of the thermoacidophilic archaeon, sulfolobus sp. strain 7. ii. characterization of the archaeal terminal oxidase subcomplexes and implication for the intramolecular electron transfer. | the terminal segment of the aerobic respiratory chain of the thermoacidophilic archaeon sulfolobus sp. strain 7 is an unusual caldariellaquinol oxidase supercomplex, which contains at least one b-type and three spectroscopically distinguishable a-type cytochromes, one copper, and a rieske-type fes center. in this paper, we report the purification and characterization of two different forms of the archaeal a-type cytochromes, namely, a three-subunit cytochrome a583-aa3 subcomplex and a single-sub ... | 1995 | 8537343 |
| resolution of the aerobic respiratory system of the thermoacidophilic archaeon, sulfolobus sp. strain 7. iii. the archaeal novel respiratory complex ii (succinate:caldariellaquinone oxidoreductase complex) inherently lacks heme group. | an active respiratory complex ii (succinate:quinone oxidoreductase) has been purified from tetraether lipid membranes of the thermoacidophilic archaeon, sulfolobus sp. strain 7. it consists of four different subunits with apparent molecular masses of 66, 37, 33, and 12 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the 66-kda subunit contains a covalently bound flavin, the 37-kda subunit is a possible iron-sulfur protein carrying three distinct types of epr-visible fes cluster ... | 1995 | 8537344 |
| expression and extensive characterization of a beta-glycosidase from the extreme thermoacidophilic archaeon sulfolobus solfataricus in escherichia coli: authenticity of the recombinant enzyme. | the gene coding for the beta-glycosidase from the archaeon sulfolobus solfataricus has been overexpressed in escherichia coli. the enzyme was purified to homogeneity with a rapid purification procedure employing a thermal precipitation as a crucial step. the final yield was 64% and the purification from the thermal precipitation was 5.4-fold. the expressed enzyme shows the same molecular mass, thermophilicity, thermal stability, and broad substrate specificity, with noticeable exocellobiase (glu ... | 1995 | 8541022 |