| mutual conversion of substrate specificities of thermoactinomyces vulgaris r-47 alpha-amylases tvai and tvaii by site-directed mutagenesis. | thermoactinomyces vulgaris r-47 produces two alpha-amylases, tvai and tvaii, differing in substrate specificity from each other. tvai favors high-molecular-weight substrates like starch, and scarcely hydrolyzes cyclomaltooligosaccharides (cyclodextrins) with a small cavity. tvaii favors low-molecular-weight substrates like oligosaccharides, and can efficiently hydrolyze cyclodextrins with various sized cavities. to understand the relationship between the structure and substrate specificity of th ... | 2003 | 12860426 |
| crystallization and preliminary x-ray analysis of substrate complexes of leucine dehydrogenase from thermoactinomyces intermedius. | leucine dehydrogenase is an octameric enzyme which belongs to the superfamily of amino-acid dehydrogenases and catalyses the reversible oxidative deamination of leucine to 2-ketoisocaproate, with the corresponding reduction of the cofactor nad(+). catalysis by this enzyme is thought to involve a large-scale motion of the enzyme's two domains between an 'open' and 'closed' form, with the latter representing a conformation of the enzyme in which the partners involved in the hydride-transfer reacti ... | 2002 | 12037317 |
| crystal structures and structural comparison of thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) at 1.6 a resolution and alpha-amylase 2 (tvaii) at 2.3 a resolution. | the x-ray crystal structures of thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) and alpha-amylase 2 (tvaii) have been determined at 1.6 a and 2.3 a resolution, respectively. the structures of tvai and tvaii have been refined, r-factor of 0.182 (r(free)=0.206) and 0.179 (0.224), respectively, with good chemical geometries. both tvai and tvaii have four domains, n, a, b and c, and all very similar in structure. however, there are some differences in the structures between them. domain n of ... | 2002 | 12051850 |
| improved molecular identification of thermoactinomyces spp. associated with mushroom worker's lung by 16s rdna sequence typing. | mushroom worker's lung (mwl) is a hypersensitivity pneumonitis or allergic alveolitis caused by a type iii igg-mediated immunopathogenic inflammatory reaction in the host due to the inhalation of several thermophilic organisms, including thermoactinomyces spp. it is difficult to distinguish phenotypically the eight species of this genus; therefore, this study sought to develop an improved molecular means of identifying thermoactinomyces spp. associated with mwl by partial 16s rdna pcr amplificat ... | 2002 | 12466412 |
| transfer of 'thermoactinomyces glaucus' ifo 12530 and 'thermoactinomyces monosporus' ifo 14050 to the genus saccharomonospora as members of saccharomonospora glauca. | two available strains of 'thermoactinomyces glaucus' and 'thermoactinomyces monosporus', 't. glaucus' ifo 12530 and 't. monosporus' ifo 14050, were considered not to be members of the genus thermoactinomyces and that they belonged to the genus saccharomonospora on the basis of the colors of colonies and 16s rdna sequences. some chemotaxonomic characteristics also showed that the two strains belong to the genus saccharomonospora. the two strains contained meso-diaminopimelic acid, galactose, and ... | 2000 | 12483576 |
| 3d structure of sulfolobus solfataricus carboxypeptidase developed by molecular modeling is confirmed by site-directed mutagenesis and small angle x-ray scattering. | sulfolobus solfataricus carboxypeptidase (cpsso) is a thermostable zinc-metalloenzyme with a m(r) of 43,000. taking into account the experimentally determined zinc content of one ion per subunit, we developed two alternative 3d models, starting from the available structures of thermoactinomyces vulgaris carboxypeptidase (model a) and pseudomonas carboxypeptidase g2 (model b). the former enzyme is monomeric and has one metal ion in the active site, while the latter is dimeric and has two bound zi ... | 2003 | 12885660 |
| cellular basis of chronic obstructive pulmonary disease in horses. | chronic obstructive pulmonary disease (copd) is an inflammatory obstructive disease of the airways characterized with hypersensitivity of the airway tissues to various allergens, most commonly the fungi contained in the poor-quality hay and straw bedding-saccharopolyspora rectivirgula, aspergillus fumigatus, and thermoactinomyces vulgaris. it is manifested clinically in middle-aged horses with recurrent episodes of dyspnea, chronic cough, and their reduced athletic and working capacity. pulmonar ... | 2007 | 17280899 |
| antigenic structure of the actinomycetales. vii. chemical and serological similarities of cell walls from 100 actinomycetales strains. | kwapinski, j. b. (the university of new england, armidale, australia). antigenic structure of the actinomycetales. vii. chemical and serological similarities of cell walls from 100 actinomycetales strains. j. bacteriol. 88:1211-1219. 1964.-cell walls prepared mechanically from 100 strains of actinomycetales were studied by chromatographic and serological methods. the cell walls of actinomyces were found to be serologically related to those of the corynebacteria and to some strains of mycobacteri ... | 1964 | 14234773 |
| purification, characterization, and subsite affinities of thermoactinomyces vulgaris r-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylases. | a maltooligosaccharide-metabolizing enzyme from thermoactinomyces vulgaris r-47 (tga) homologous to glucoamylases does not degrade starch efficiently unlike most glucoamylases such as fungal glucoamylases (uotsu-tomita et al., appl. microbiol. biotechnol., 56, 465-473 (2001)). in this study, we purified and characterized tga, and determined the subsite affinities of the enzyme. the optimal ph and temperature of the enzyme are 6.8 and 60 degrees c, respectively. activity assays with 0.4% substrat ... | 2004 | 14981306 |
| complex structures of thermoactinomyces vulgaris r-47 alpha-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism. | thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii) has the unique ability to hydrolyze cyclodextrins (cds), with various sized cavities, as well as starch. to understand the relationship between structure and substrate specificity, x-ray structures of a tvaii-acarbose complex and inactive mutant tvaii (d325n/d421n)/alpha-, beta- and gamma-cds complexes were determined at resolutions of 2.9, 2.9, 2.8, and 3.1 a, respectively. in all complexes, the interactions between ligands and enzymes at ... | 2004 | 15138257 |
| the crystal structure of thermoactinomyces vulgaris r-47 alpha-amylase ii (tva ii) complexed with transglycosylated product. | alphan alpha-amylase (tva ii) from thermoactinomyces vulgaris r-47 efficiently hydrolyzes alpha-1,4-glucosidic linkages of pullulan to produce panose in addition to hydrolyzing starch. tva ii also hydrolyzes alpha-1,4-glucosidic linkages of cyclodextrins and alpha-1,6-glucosidic linkages of isopanose. to clarify the basis for this wide substrate specificity of tva ii, we soaked 4(3)-alpha-panosylpanose (4(3)-p2) (a pullulan hydrolysate composed of two panosyl units) into crystals of d325n inacti ... | 2004 | 15182368 |
| ca2+ dependence and inhibitory effects of trifluoperazine on plasma membrane atpase of thermoactinomyces vulgaris. | ca2+ enhanced the plasma membrane ca(2+)-atpase (pmca) specific activities in wild-type strain 1227 and mutant strains 1278, 1286, and 1261 of thermoactinomyces vulgaris. the ca(2+)-atpase specific activities showed marked increase with increasing concentrations of ca2+ added in the form of cacl2 in the culture medium and reached the optimum values at 0.6 mm in strains 1227, 1278, and 1286 and at 0.7 mm in strain 1261 of t. vulgaris. trifluoperazine, a specific blocker of calmodulin, when added ... | 2004 | 15297926 |
| exposure to bioaerosols in a municipal sewage treatment plant. | microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ... | 2003 | 14677919 |
| cellulolytic enzyme system of thermoactinomyces sp. grown on microcrystalline cellulose. | the carboxymethyl-cellulase and avicelase activities of thermoactinomyces sp., strain yx, were produced simultaneously with cell growth. throughout the growth phase these activities were primarily extracellular, with up to 50% adsorbed to residual cellulosic substrate at any one time. on the other hand, the beta-glucosidase activity was associated with the culture solids throughout the entire fermentation and appears to be intracellular. preparative isoelectric focusing of the culture filtrate, ... | 1978 | 16345322 |
| survival of microorganisms in a rock bed under conditions simulating solar heat storage. | a laboratory-scale unit containing about 360 kg of washed river gravel was designed to [ill] the use of rocks for heat storage. the unit was operated under varying conditions of temperature, relative humidity, and the addition of volatile nutrients over a 4-month period. effluent air and rock surfaces were monitored for the presence of microorganisms. after 2 weeks, virtually no microorganisms were detected in the effluent air except when dry soil or compost was added as the inoculum. a small nu ... | 1981 | 16345765 |
| use of epifluorescence microscopy in studies of the germination and recovery of thermoactinomycetes. | spore germination and growth of thermoactinomycetes were observed by epifluorescence microscopy. each of the principal stages in germination was recognized and found to correspond to changes in phase-contrast appearance commonly monitored during endospore germination. the effects of novobiocin and nalidixic acid on germination of thermoactinomyces vulgaris and t. thalpophilus were studied by using epifluorescence microscopy. outgrowth but not initiation was inhibited by 100 mug of nalidixic acid ... | 1982 | 16345954 |
| partial purification and characterization of a thermostable actinomycete beta-amylase. | a thermostable amylase, possibly a beta-amylase from thermoactinomyces sp. no. 2 isolated from soil, is reported. the enzyme was purified 36-fold by acetone precipitation, ion-exchange chromatography, and sephadex g-200 gel filtration, and the molecular weight was estimated at 31,600. the enzyme was characterized by demonstration of optimum activity at 60 degrees c and ph 7 and by retention of 70% activity at 70 degrees c (30 min). it was stimulated by mn and fe but strongly inhibited by hg. mal ... | 1984 | 16346495 |
| identification and characteristics of actinomycetes useful for semicontinuous treatment of domestic animal feces. | selected strains of actinomycetes useful for practicing semicontinuous treatment of swine and poultry feces were identified as streptomyces antibioticus s-4, s. puniceus n-50-2, s. nigrifaciens n-9-3, thermoactinomyces vulgaris hir-60, and thermomonospora viridis hir-50. these five obligately aerobic strains grew preferably on nonsterilized fresh swine feces in 24 h without any additives. they assimilated offensive volatile fatty acids in the swine and poultry feces. cultures of these five strai ... | 1988 | 16347716 |
| temperature impacts the multiple attack action of amylases. | the action pattern of several amylases was studied at 35, 50, and 70 degrees c using potato amylose, a soluble (red starch) and insoluble (cross-linked amylose) chromophoric substrate. with potato amylose as substrate, bacillus stearothermophilus alpha-amylase (bsta) and porcine pancreatic alpha-amylase displayed a high degree of multiple attack (dma, i.e., the number of bonds broken during the lifetime of an enzyme-substrate complex minus one), the fungal alpha-amylase from aspergillus oryzae a ... | 2007 | 17309295 |
| quantitative assessment of factors affecting the recovery of indigenous and released thermophilic bacteria from compost. | thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-andersen sampler methods. excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result was largely unaffected by the use of modified extraction procedures and diluents. assessment of the actinomycete population was more successfully achieved by applying the sedimentatio ... | 1988 | 16347800 |
| crystallization and preliminary x-ray analysis of thermoactinomyces vulgaris r-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase. | a maltooligosaccharide-metabolizing enzyme from thermoactinomyces vulgaris r-47 (tga) homologous to glucoamylase degrades maltooligosaccharides more efficiently than starch, unlike fungal glucoamylases. tga was crystallized and the state of the protein in solution was analyzed by gel-filtration chromatography. diffraction data were collected to 3.31 a resolution. the tga crystal belongs to the orthorhombic space group p2(1)2(1)2(1) or p2(1)2(1)2, with unit-cell parameters a = 110.2, b = 317.6, c ... | 2005 | 16511025 |
| structure of a complex of thermoactinomyces vulgaris r-47 alpha-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft. | thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii) can efficiently hydrolyze both starch and cyclomaltooligosaccharides (cyclodextrins). the crystal structure of an inactive mutant tvaii in a complex with maltohexaose was determined at a resolution of 2.1a. tvaii adopts a dimeric structure to form two catalytic sites, where substrates are found to bind. at the catalytic site, there are many hydrogen bonds between the enzyme and substrate at the non-reducing end from the hydrolyzing site, bu ... | 2006 | 16564038 |
| studies on the thermophilic actinomycetes. i. methods of cultivation. | a total of 1,000 isolates of thermophilic actinomycetes representing two genera, streptomyces and thermoactinomyces, were studied. media for cultivation and for physiological studies were designed. differences between the two genera are noted and taxonomic criteria for the genus thermoactinomyces are suggested. the importance of the nutritional environment to the thermophilic habit is noted. | 1961 | 13775873 |
| structural basis for cyclodextrin recognition by thermoactinomyces vulgaris cyclo/maltodextrin-binding protein. | the crystal structure of a thermoactinomyces vulgaris cyclo/maltodextrin-binding protein (tvucmbp) complexed with gamma-cyclodextrin has been determined. like escherichia coli maltodextrin-binding protein (ecombp) and other bacterial sugar-binding proteins, tvucmbp consists of two domains, an n- and a c-domain, both of which are composed of a central beta-sheet surrounded by alpha-helices; the domains are joined by a hinge region containing three segments. gamma-cyclodextrin is located at a clef ... | 2007 | 17371546 |
| tryptamine derived amides with thiazole ring system from thermoactinomyces strain ta66-2. | a moderately thermophilic actinomycete strain, which was identified as thermoactinomyces strain ta66-2, was isolated from hot-spring water. fermentation, followed by solvent partition and chromatographic separations, resulted in the isolation of two new and two known molecules. the structures of the new compounds were elucidated as 2-(1-propionylaminoethyl)thiazole-4-carboxylic acid [2-(1h-indol-3-yl)ethyl]amide and 2-(1-acetylaminoethyl)thiazole-4-carboxylic acid [2-(1h-indol-3-yl)-ethyl]amide ... | 2008 | 18041008 |
| identification and characterization of a novel spore-associated subtilase from thermoactinomyces sp. cdf. | a gene encoding a spore-associated subtilase, designated protease cdf, was cloned from thermoactinomyces sp. cdf and expressed in escherichia coli. the enzyme gene is translated as a proform consisting of a 94 aa propeptide and a 283 aa mature protease domain. phylogenetic analysis revealed that this enzyme belonged to the subtilisin family, but could not be grouped into any of its six known subfamilies. the mature protease cdf has an unusually high content of charged residues, which are mainly ... | 2009 | 19696109 |
| val326 of thermoactinomyces vulgaris r-47 amylase ii modulates the preference for alpha-(1,4)- and alpha-(1,6)-glycosidic linkages. | thermoactinomyces vulgaris r-47 alpha-amylase ii (tva ii) catalyzes not only the hydrolysis of alpha-(1,4)- and alpha-(1,6)-glycosidic linkages but also transglycosylation. the subsite +1 structure of alpha-amylase family enzymes plays important roles in substrate specificity and transglycosylation activity. we focused on the amino acid residue at the 326th position based on information on the primary structure and crystal structure, and replaced val with ala, ile, or thr. the v326a mutant favor ... | 2007 | 17400040 |
| bioactive rare actinomycetes from indigenous natural substrates of andhra pradesh. | this investigation reports the occurrence of bioactive rare actinomycetes from different indigenous natural substrates of andhra pradesh. during the course of our investigation on 20 terrestrial soil samples, 5 marine samples and 3 fresh water samples, a total of 92 rare actinomycetes belonging to micromonospora, nocardia, actinomadura and thermoactinomyces genera were isolated. the antimicrobial and enzymatic activities were studied for all the isolates. the antibacterial and antifungal activit ... | 2002 | 15061589 |
| [a case of farmer's lung disease manifested by smoking cessation]. | a 40-year-old man, a cattle hoof-chipper, was admitted to hokkaido university hospital in june 2002 for a workup of the occasional fever, cough and dyspnea that had affected him at work since march 2002. he had not smoked since january 2001. on admission, he had a marked increase in the proportion of lymphocytes (62.9%) and the cd 4/cd 8 ratio (3.0) in the bronchoalveolar lavage fluid. serum precipitating antibody against thermoactinomyces vulgaris was detected. the results of a transbronchial l ... | 2004 | 15114848 |
| structural principles of the wide substrate specificity of thermoactinomyces vulgaris carboxypeptidase t. reconstruction of the carboxypeptidase b primary specificity pocket. | site-directed mutagenesis in the active site of thermoactinomyces vulgaris carboxypeptidase t (cpt), which is capable of hydrolyzing both hydrophobic and positively charged substrates, resulted in five mutants: cpt1 (a243g), cpt2 (d253g/t255d), cpt3 (a243g/d253g/t255d), cpt4 (g207s/a243g/d253g/t255d), and cpt5 (g207s/a243g/t250a/d253g/t255d). these mutants step-by-step reconstruct the primary specificity pocket of carboxypeptidase b (cpb), which is capable of cleaving only positively charged c-t ... | 2007 | 17511606 |
| mouse model of hypersensitivity pneumonitis after inhalation exposure to different microbial antigens associated with organic dusts. | the aim of this study was to reproduce in laboratory conditions hypersensitivity pneumonitis (hp) pathogenesis in a new animal model predictive of the human response, and to select the microbial antigen associated with organic dust that exerts the strongest pathogenic effect on the respiratory organ. to achieve this goal, mice of the strain c57bl/6j prone to fibrosis were exposed for 1 hour daily up to 28 days to the inhalation of aerosols of each of the 5 microbial components of organic dusts w ... | 2011 | 21736281 |
| polyester-degrading thermophilic actinomycetes isolated from different environment in taiwan. | thermophilic actinomycetes strains were isolated from various environment in taiwan and screened for degradation of poly(ethylene succinate) (pes), poly(epsilon-caprolactone) (pcl) and/or poly(beta-hydroxybutyrate) (phb) by the clear-zone method. out of 341 strains of thermophilic actinomycetes, 105 isolates were phb-degraders (30.8%), 198 isolates were pcl-decomposers (58.1%), and 99 isolates could degrade pes (29.0%). furthermore, 77 isolates could degrade both phb and pcl (22.6%), 35 isolates ... | 2007 | 17653512 |
| mg2+ decreases arrhenius energies of activation for high temperature catalysis of phosphatases in thermoactinomyces vulgaris. | the nonspecific acid and alkaline phosphatases of thermoactinomyces vulgaris were found to be optimally active at 65 degrees c and 70 degrees c, respectively, indicating the thermophilic nature of these enzymes in this obligate thermophile. mg(2+), when added in the assay mixture (in the form of mgcl(2)), increased the specific activities of these enzymes without affecting their respective temperature optima. this divalent cation decreased the arrhenius energies of activation (e ( a )) of both a ... | 2007 | 17657541 |
| a novel neutral protease from thermoactinomyces species 27a: sequencing of the gene, purification, and characterization of the enzyme. | the nucleotide sequence of the previously cloned (zabolotskaya, m. v., nosovskaya, e. a., kaplun, m. a., and akimkina, t. v. (2001). mol. gen. mikrobiol. virusol. no 1, 32-34) dna fragment from thermoactinomyces sp. 27a (genbank accession no. ay280367) containing the metalloproteinase gene was determined. a continuous open reading frame encoding a polypeptide of 673 aa was revealed. analysis of this sequence demonstrated that the metalloproteinase from thermoactinomyces sp. 27a is synthesized as ... | 2004 | 15635941 |
| proposal of the genus thermoactinomyces sensu stricto and three new genera, laceyella, thermoflavimicrobium and seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. | phylogenetic analysis based on 16s rrna gene sequences revealed that thermoactinomyces species with validly published names can be assigned to four clusters or lineages. the type strains of thermoactinomyces sacchari and thermoactinomyces putidus were differentiated from the type strains of thermoactinomyces vulgaris and thermoactinomyces intermedius by the predominant menaquinone and fatty acid profiles. the type strains of thermoactinomyces dichotomicus and thermoactinomyces peptonophilus form ... | 2005 | 15653907 |
| in vitro refolding of carboxypeptidase t precursor from thermoactinomyces vulgaris obtained in escherichia coli as cytoplasmic inclusion bodies. | carboxypeptidase t precursor from thermoactinomyces vulgaris, which fails to contain its own leader peptide, has been expressed in escherichia coli as insoluble cytoplasmic inclusion bodies. the yield of a washed recombinant protein from 1 l of culture liquid was about 60 mg. the obtained inclusion bodies were denatured in 6 m guanidine-hcl and then renatured by a rapid dilution. the important role of calcium for the complete stabilization of the refolded carboxypeptidase t precursor was establi ... | 2005 | 15721771 |
| novel thermostable serine collagenase from thermoactinomyces sp. 21e: purification and some properties. | a thermophilic actinomycete strain thermoactinomyces sp. 21e producing a highly thermostable serine collagenase was isolated from bulgarian soil. the collagenase, produced extracellular by thermoactinomyces sp. 21e, was purified to homogeneity by heat treatment, ultrafiltration, saturation with ammonium sulfate and gel filtration chromatography with a 101-fold increase in specific activity and 58% recovery. the collagenase has a relative molecular mass of 50000 by sds-page. the optimum temperatu ... | 2006 | 16847831 |
| mechercharmycins a and b, cytotoxic substances from marine-derived thermoactinomyces sp. ym3-251. | a new cytotoxic substance named mechercharmycin a was isolated from marine-derived thermoactinomyces sp. ym3-251. the structure of mechercharmycin a was determined by an x-ray crystallographic analysis to be cyclic peptide-like and bearing four oxazoles and a thiazole. mechercharmycin b, a linear congener of mechercharmycin a, was also isolated from the same bacterium. mechercharmycin a exhibited relatively strong antitumor activity, whereas mechercharmycin b exhibited almost no such activity. | 2005 | 15981418 |
| [the phylogenetic diversity of aerobic organotrophic bacteria from the dagan high-temperature oil field]. | the distribution and species diversity of aerobic organotrophic bacteria in the dagan high-temperature oil field (china), which is exploited via flooding, have been studied. twenty-two strains of the most characteristic thermophilic and mesophilic aerobic organotrophic bacteria have been isolated from the oil stratum. it has been found that, in a laboratory, the mesophilic and thermophilic isolates grow in the temperature, ph, and salinity ranges characteristic of the injection well near-bottom ... | 2005 | 16119855 |
| occurrence and characterization of actinobacteria and thermoactinomycetes isolated from pulp and board samples containing recycled fibres. | the aim of this study was to characterize the actinobacterial population present in pulps and boards containing recycled fibres. a total of 107 isolates was identified on the basis of their pigmentation, morphological properties, fatty acid profiles and growth temperature. of the wet pulp and water sample isolates (n=87), 74.7% belonged to the genus streptomyces, 17.2% to nocardiopsis and 8.0% to thermoactinomycetes, whereas all the board sample isolates (n=20) were thermoactinomycetes. the iden ... | 2006 | 16292555 |
| [a case of hypersensitity pneumonitis due to the contamination of a dehumidifier by thermoactinomyces vulgaris]. | a 22-year-old man was admitted to our hospital because of high fever and shortness of breath. a chest ct showed centrilobular nodules and ground-glass attenuation in both lungs. transbronchial lung biopsy specimens showed alveolitis including the infiltration of mononuclear cells and granulomas. he responded to treatment, but upon returning home the radiological findings and clinical symptoms reappeared. we focused on a dehumidifier because it had been used continuously in his room. thermoactino ... | 2008 | 18260309 |
| structural principles of the broad substrate specificity of thermoactinomyces vulgaris carboxypeptidase t--role of amino acid residues at positions 260 and 262. | an influence of residues at positions 260 and 262 on a broad substrate specificity of thermoactinomyces vulgaris carboxypeptidase t (cpt) has been studied by means of site-directed mutagenesis. the structure of the s1'-site of cpt is similar to those of pancreatic carboxypeptidases a (cpa) and b (cpb); however, the enzyme is capable of cleaving off c-terminal hydrophobic (like cpa), c-terminal positively charged (like cpb), and negatively charged residues. the spatial alteration of the s1' site ... | 2008 | 18515300 |
| leu254 residue and calcium ions as new structural determinants of carboxypeptidase t substrate specificity. | new determinants of thermoactinomyces vulgaris carboxypeptidase t (cpt) substrate specificity--structural calcium ions and leu254 residue--were found by means of steady-state kinetics and site-directed mutagenesis. the removal of calcium ions shifted the selectivity profile of hydrolysis of tripeptide substrates with c-terminal leu, glu, and arg from 64/1.7/1 to 162/1.3/1. substitution of the hydrophobic leu254 in cpt for polar asn did not change hydrolysis efficiency of substrates with c-termin ... | 2008 | 18991561 |
| desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family thermoactinomycetaceae matsuo et al. 2006. | a novel gram-positive, aerobic, catalase-positive, filamentous micro-organism, designated strain immib l-1269(t), originating from sputum was characterized using phenotypic and molecular taxonomic methods. it showed cell-wall chemotype iii, phospholipid type pii (with phosphatidylethanolamine as the diagnostic phospholipid) and contained an unsaturated menaquinone with seven isoprene units (mk-7) as the predominant isoprenoid quinone. it synthesized long-chain cellular fatty acids of the straigh ... | 2009 | 19244421 |
| detection and molecular characterization of filamentous actinobacteria and thermoactinomycetes present in water-damaged building materials. | in this study the dominant filamentous actinobacteria occurring in water-damaged building materials were detected by culture and characterized by automated ribotyping and 16s rrna gene sequencing. fifty-two samples were taken from 20 water-damaged houses in four different countries. a total of 122 bacterial isolates were analyzed. actinobacteria or thermoactinomycetes were present in 48% of the samples. the dominant genus was streptomyces (58% of isolates), followed by thermoactinomyces (23%), l ... | 2009 | 19302504 |
| degradation of rutin by thermoactinomyces vulgaris and other thermophilic compost isolates. | the mutagenic effects of rutin and quercetin have aroused the interest of many investigators. to develop the microbial degradation of rutin, a thermophilic actinomycete, which could hydrolyze rutin, was isolated from compost soil. the taxonomical characteristics of this thermophilic actinomycete were examined and identified as thermoactinomyces vulgaris pu18-2. after cultivation of t. vulgaris pu18-2 in the rutin-cyc medium for 60 h, the culture filtrate had a rutin-degrading ability, but the ce ... | 2009 | 19489631 |
| crystal structures of open and closed forms of cyclo/maltodextrin-binding protein. | the crystal structures of thermoactinomyces vulgaris cyclo/maltodextrin-binding protein (tvucmbp) complexed with alpha-cyclodextrin (alpha-cd), beta-cyclodextrin (beta-cd) and maltotetraose (g4) have been determined. a common functional conformational change among all solute-binding proteins involves switching from an open form to a closed form, which facilitates transporter binding. escherichia coli maltodextrin-binding protein (ecombp), which is structurally homologous to tvucmbp, has been det ... | 2009 | 19490104 |
| diversity of bacteria and fungi in aerosols during screening in a green waste composting plant. | this article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. the structure of the microbial community was examined using a fingerprint technique and dna libraries. the results show: (i) the very high diversity of bacteria and fungi in aerosols produced during the composting screening stage ... | 2009 | 19533230 |
| the microbial signature of aerosols produced during the thermophilic phase of composting. | the microbial diversity of bioaerosols released during operational activities at composting plants is poorly understood. identification of bacteria and fungi present in such aerosols is the prerequisite for the definition of microbial indicators that could be used in dispersal and exposure studies. | 2010 | 19602015 |
| alkalithermophilic actinomycetes in a subtropical area of jujuy, argentina. | the objective of this study was to examine the alkalithermophilic actinomycete communities in the subtropical environment of jujuy, argentina, characterized by sugarcane crops. laceyella putida, laceyella sacchari, thermoactinomyces intermedius, thermoactinomyces vulgaris and thermoflavimicrobium dichotomicum were isolated on the media with novobiocin, from sugar cane plants and renewal rhizospheres, and grass and wood soils. soil ph was almost neutral or lightly alkaline, except for grass soil ... | 2009 | 19623902 |
| respiratory health effects and immunological response to thermoactinomyces among sugar cane workers in nicaragua. | specific sensitization and respiratory effects associated with the inhalation of sugar cane dust were evaluated in a group of 51 nicaraguan workers exposed to bagasse. a questionnaire interview, lung function test, serum precipitin tests for thermoactinomyces sacchari and t. vulgaris, and immunoglobulin e tests for specific environmental allergens were performed for each worker. twenty-one workers reported at least one respiratory symptom and 16 reported possible symptoms of bagassosis. six work ... | 2009 | 19650579 |
| inoculation of food waste with the thermo-tolerant lipolytic actinomycete thermoactinomyces vulgaris a31 and maturity evaluation of the compost. | the ability of the thermo-tolerant lipolytic actinomycete, thermoactinomyces vulgaris a31, to efficiently decompose food waste into mature compost was studied. using a range of chemical parameters (ph, total organic carbon content (toc), total nitrogen content, c/n ratio), co(2) evolution, enzymatic activities (dehydrogenase, polyphenol oxidase, urease) and germination assays, the composition, stability and maturity of the compost produced were assessed. inoculation reduced crude fat and decreas ... | 2010 | 20462753 |
| [case of bird-related hypersensitivity pneumonitis in a farmer]. | a 48-year-old female farmer presented to our hospital with shortness of breath and cough. her partial pressure of arterial oxygen (pao2) level on room air was 58.4torr, and chest computed tomography (ct) showed centrilobular ground-glass micronodules. she was admitted for further evaluation. after admission, symptoms, pao2, and radiological findings improved without any treatment; however, her symptoms recurred when she returned to work. exposure to her house did not elicit any symptomatic respo ... | 2010 | 21226310 |
| production and partial characterization of high molecular weight extracellular alpha-amylase from thermoactinomyces vulgaris isolated from egyptian soil. | optimizing production of alpha-amylase production by thermoactinomyces vulgaris isolated from egyptian soil was studied. the optimum incubation period, temperature and initial ph of medium for organism growth and enzyme yield were around 24 h, 55 degrees c and 7.0, respectively. maximum alpha-amylase activity was observed in a medium containing starch as carbon source. the other tested carbohydrates (cellulose, glucose, galactose, xylose, arabinose, lactose and maltose) inhibited the enzyme prod ... | 2011 | 21630576 |
| enzymatic synthesis of novel branched sugar alcohols mediated by the transglycosylation reaction of pullulan-hydrolyzing amylase ii (tva ii) cloned from thermoactinomyces vulgaris r-47. | transglycosylation reactions are useful for preserving a specific sugar structure during the synthesis of branched oligosaccharides. we have previously reported a panosyl unit transglycosylation reaction by pullulan-hydrolyzing amylase ii (tva ii) cloned from thermoactinomyces vulgaris r-47 (tonozuka et al., carbohydr. res., 1994, 261, 157-162). the acceptor specificity of the tva ii transglycosylation reaction was investigated using pullulan as the donor and sugar alcohols as the acceptor. tva ... | 2011 | 21722879 |
| involvement of propeptides in formation of catalytically active metalloproteinase from thermoactinomyces sp. | the metalloproteinase from thermoactinomyces sp. 27a (mpr) represents secretory thermolysin-like metalloproteinases of the m4 family. the thermoactinomyces enzyme is synthesized as a precursor consisting of a signal peptide, n-terminal propeptide, mature region, and c-terminal propeptide. the functional molecule lacks the signal peptide, n-terminal propeptide, and c-terminal propeptide, which indicates the processing of these regions. until now, it remained unclear if the n-terminal propeptide i ... | 2011 | 21675948 |