| [actinomycetomas in senegal: study of 90 cases]. | mycetoma is a pathological process during which eumycetomic (fungal) or actinomycotic causative agents from exogenous source produce grains. medical treatment is available for actinomycetomas and surgery is still the main treatment for eumycetoma. we report 90 cases of actinomycetoma occuring in male adult patients coming mainly from central senegal. patients living far from health structures consult after a long evolution period for tumors and pains which prevent them from carrying out their ac ... | 2005 | 15915967 |
| antibiotics ge23077, novel inhibitors of bacterial rna polymerase. ii. structure elucidation. | during the screening program for new antibacterial agents produced by actinomycetes, ge23077 was isolated from fermentation broths of an actinomadura sp. strain as a complex of factors a1, a2, b1, b2. nmr, ms and gc/ms analysis carried out on the isolated components led to the conclusion that ge23077 is a novel cyclic heptapeptide consisting of common and unusual amino acids. the chemical structures of the complex components were elucidated. components a and b differ in the structure of the acyl ... | 2005 | 15981412 |
| crystal structure of the actinomadura r39 dd-peptidase reveals new domains in penicillin-binding proteins. | actinomadura sp. r39 produces an exocellular dd-peptidase/penicillin-binding protein (pbp) whose primary structure is similar to that of escherichia coli pbp4. it is characterized by a high beta-lactam-binding activity (second order rate constant for the acylation of the active site serine by benzylpenicillin: k2/k = 300 mm(-1) s(-1)). the crystal structure of the dd-peptidase from actinomadura r39 was solved at a resolution of 1.8 angstroms by single anomalous dispersion at the cobalt resonance ... | 2005 | 15987687 |
| antibiotics ge23077, novel inhibitors of bacterial rna polymerase. part 3: chemical derivatization. | ge23077 is a novel rna polymerase inhibitor that is isolated from the fermentation broth of an actinomadura sp. it is a cyclic heptapeptide complex made up of four factors, differing in the structure of acyl group connected to the side chain of an alpha,beta-diaminopropanoic acid moiety and in the configuration of the stereocenter of an alpha-amino-malonic acid residue. although ge23077 shows strong inhibitory activity on both rifampicin-sensitive and -resistant polymerases, it exhibits poor ant ... | 2005 | 15990299 |
| intracranial actinomadura granuloma. | | 2005 | 16230821 |
| isolation of novel bacteria and actinomycetes using soil-extract agar medium. | novel bacteria were discovered using an isolation technique consisting of (i) selection of microorganisms that grew on soil-extract agar medium, but not on conventional media, and (ii) detection of small microbial colonies with a microscope. three bacterial strains thus isolated were provisionally designated shinshu-th1, -th2, -th 3, and five actinomycete strains, shinshu-ms-01, -02, -03, -04, -05, respectively. sequence analysis of their 16s rdna showed that th1 had 95--96% homology with three ... | 2005 | 16233821 |
| [cranial and cervical vertebral mycetoma. report of three cases]. | mycetomas inflammatory-like tumors presenting as fistulas found in the skin and soft tissues. inoculation, generally in tropical areas, occurs by skin injury. the foot is the main infection site. other uncommon infection sites include the cranial and cervical areas which are rare. we present three cases of cranial and cervical mycetoma. clinical sign were dominated by headache, cervical pain and cervico-occipital tumefaction with formation of pus and granules (red in two cases, black in one). a ... | 2005 | 16327680 |
| actinomadura hallensis sp. nov., a novel actinomycete isolated from mt. halla in korea. | a novel actinomycete, strain h647-1t, which was isolated from soil of mt. halla, jeju island, republic of korea, was subjected to phenotypic and genotypic characterization. the organism, which produces warty spore chains arranged in hooks or spirals on the aerial mycelium, was shown to have chemotaxonomic characteristics consistent with assignment to the genus actinomadura. a comparative analysis of 16s rrna gene sequences indicated that the organism formed a distinct clade within the evolutiona ... | 2006 | 16403895 |
| a rare european case of madura foot due to actinomycetes. | we present a case of mycetoma by actinomadura spp. on the foot of an albanian young man arrived to our observation approximately 5 years after the first clinical manifestations (hard tumefaction, slightly painful upon weight-bearing and palpation and cutaneous fistulas that discharged an abundant granulomatous secretion). direct microscopic analysis and culture of the white-yellowish grains included gram staining, which showed extensively branched gram-positive hyphae less than 1 mm in diameter, ... | 2006 | 16563843 |
| identification of poly(cis-1,4-isoprene) degradation intermediates during growth of moderately thermophilic actinomycetes on rubber and cloning of a functional lcp homologue from nocardia farcinica strain e1. | the enrichment and isolation of thermophilic bacteria capable of rubber [poly(cis-1,4-isoprene)] degradation revealed eight different strains exhibiting both currently known strategies used by rubber-degrading mesophilic bacteria. taxonomic characterization of these isolates by 16s rrna gene sequence analysis demonstrated closest relationships to actinomadura nitritigenes, nocardia farcinica, and thermomonospora curvata. while strains related to n. farcinica exhibited adhesive growth as describe ... | 2006 | 16672480 |
| antimicrobial compounds produced by actinomadura sp. ac104 isolated from an algerian saharan soil. | during a search for nonpolyenic antifungal antibiotics, an actinomycete designated ac104 was isolated from a saharan soil sample by a dilution agar plating method using a chitin - vitamins b medium supplemented with rifampicin. isolate ac104 presented the morphological and the chemical characteristics of the genus actinomadura. on the basis of 76 physiological tests and 16s rdna analysis, this isolate was determined to be quite different from the known species of actinomadura. it is active again ... | 2006 | 16699588 |
| [clinical and microbiological study of mycetomas at the muñiz hospital of buenos aires between 1989 and 2004]. | this work presents clinical, microbiological and outcome data collected from 76 patients with mycetomas at the muñiz hospital from 1989 to 2004. forty-nine patients were male and 27 female; the mean age was 43.4 years. the majority of the patients acquired the infection in argentina: the most affected provinces were santiago del estero with 31 cases, and chaco with 11; 8 cases came from other countries (bolivia 6 and paraguay 2). the mean evolution of the disease was 9.2 years. the most frequent ... | 2006 | 16784127 |
| biosensor analysis of beta-lactams in milk using the carboxypeptidase activity of a bacterial penicillin binding protein. | the applicability of a beta-lactam receptor protein for detection of beta-lactam antibiotics in milk using surface plasmon resonance (spr) biosensor technology was investigated. the advantage of using a receptor protein instead of antibodies for detection of beta-lactams is that a generic assay, specific for the active form of the beta-lactam structure, is obtained. two assays based on the enzymatic activity of the dd-carboxypeptidase from actinomadura r39 were developed, using a biacore spr bio ... | 2006 | 16792082 |
| majority of actinomadura clinical isolates from sputa or bronchoalveolar lavage fluid in japan belongs to the cluster of actinomadura cremea and actinomadura nitritigenes, and the description of actinomadura chibensis sp. nov. | in japan during 1996-2004, 21 actinomycete strains that have madurose as the diagnostic cell-wall sugar and show true branching in their substrate and aerial mycelia were isolated from sputa or bronchoalveolar lavage (bal) fluid of patients with pulmonary infections or who were suspected of having related infections. chemotaxonomic studies showed that all the isolates belong to the genus actinomadura. among them, six and seven strains were classified respectively into clusters of actinomadura ni ... | 2006 | 17039274 |
| reactivity of penicillin-binding proteins with peptidoglycan-mimetic beta-lactams: what's wrong with these enzymes? | beta-lactams exert their antibiotic action through their inhibition of bacterial dd-peptidases (penicillin-binding proteins). bacteria, in general, carry several such enzymes localized on the outside of their cell membrane to catalyze the final step in cell wall (peptidoglycan) synthesis. they have been classified into two major groups, one of high molecular weight, the other of low. members of the former group act as transpeptidases in vivo, and their inhibition by beta-lactams leads to cessati ... | 2006 | 17176110 |
| actinomadura rudentiformis sp. nov., isolated from soil. | as part of an antibiotic-screening programme, the actinomycete strain hmc1(t) was isolated from soil collected from the banks of the gamka river in the swartberg nature reserve, western cape province, south africa. the isolate produced branching vegetative mycelia, which entwined to form rope-like structures. it exhibited antibiosis against enterococcus faecium vana (a vancomycin-resistant clinical strain) and mycobacterium aurum a+. organic solvent extracts of the culture filtrate and mycelial ... | 2007 | 17220439 |
| biotransformations of lipoglycopeptides to obtain novel antibiotics. | the emergence of resistance among gram-positive pathogens towards glycopeptide antibiotics has stimulated the research of second-generation molecules with improved activity and expanded antimicrobial spectrum. in this paper we investigate biotransformations as a way to generate novel teicoplanin- and a40926-like molecules. a range of commercial enzymes, fungi and actinomycetes were tested on a40926 and on its semi-synthetic derivatives (mdl 63,246 and dalbavancin). oxidation of dalbavancin to md ... | 2007 | 17456978 |
| polyester-degrading thermophilic actinomycetes isolated from different environment in taiwan. | thermophilic actinomycetes strains were isolated from various environment in taiwan and screened for degradation of poly(ethylene succinate) (pes), poly(epsilon-caprolactone) (pcl) and/or poly(beta-hydroxybutyrate) (phb) by the clear-zone method. out of 341 strains of thermophilic actinomycetes, 105 isolates were phb-degraders (30.8%), 198 isolates were pcl-decomposers (58.1%), and 99 isolates could degrade pes (29.0%). furthermore, 77 isolates could degrade both phb and pcl (22.6%), 35 isolates ... | 2007 | 17653512 |
| [cloning and functional studies of the chromosomal replication origin of actinomadura yumaensis]. | the chromosomal replication origins (oric) have been investigated in gram-positive eubacteria actinomycetes, including streptomyces, mycobacterium and amycolatopsis, and reveal various dnaa-boxes and at-rich sequences between the conserved dnaa and dnan genes. actinomadura yumaensis nrrl12515 is a producer of anthelmintic polyether maduramicin. in this paper,cloning, sequencing and functional studies of its oric have been carried out. a pair of oligonucleotide primers, based on the conserved seq ... | 2007 | 17672320 |
| actinomadura alba sp. nov., isolated from soil in yunnan, china. | a novel actinomycete, strain yim 45681(t), which was isolated from soil in a suburb of kunming, yunnan province, china, was subjected to phenotypic and genotypic characterization. the micro-organism, which produces short spore chains arranged in spirals on the aerial mycelium, was shown to have meso-diaminopimelic acid in the cell wall. the sugars present in whole-cell hydrolysates were ribose, xylose, galactose, madurose and glucose. the predominant menaquinones were mk-9(h(4)), mk-9(h(6)) and ... | 2007 | 17684247 |
| [actinomycete growth in conditions of low moisture]. | actinomycete communities demonstrated a replacement of the generic composition in time as a function of soil moisture. representatives of the genera streptomyces, micromonospora, actinomadura, saccharopolyspora, and microbispora were repeatedly isolated from soil under different moisture conditions (field capacity, maximum molecular capacity, and maximum adsorption capacity). representatives of some rare genera (thermomonospora and kibdelosporangium) were isolated from soil with low moisture lev ... | 2007 | 17853691 |
| characterization of the maduropeptin biosynthetic gene cluster from actinomadura madurae atcc 39144 supporting a unifying paradigm for enediyne biosynthesis. | the biosynthetic gene cluster for the enediyne antitumor antibiotic maduropeptin (mdp) from actinomadura madurae atcc 39144 was cloned and sequenced. cloning of the mdp gene cluster was confirmed by heterologous complementation of enediyne polyketide synthase (pks) mutants from the c-1027 producer streptomyces globisporus and the neocarzinostatin producer streptomyces carzinostaticus using the mdp enediyne pks and associated genes. furthermore, mdp was produced, and its apoprotein was isolated a ... | 2007 | 17918933 |
| mycetomas in iran: a review article. | mycetomas are the subcutaneous and relatively rare chronic pustular infections. the etiologic agents of mycetomas are a group of saprophytic fungi and actinomycetes living in soil. we retrospectively discussed the overall prevalence of mycetomas and the prevalence of infective agents in iran between 1972 and 2005. seventy-six cases of mycetomas have been reported from various geographical locations in iran during 33 years. analysis of the records revealed that 84.5% were actinomycetoma and only ... | 2008 | 17922219 |
| rare actinomycete bacteria from the shallow water sediments of the trondheim fjord, norway: isolation, diversity and biological activity. | actinomycete bacteria produce a wide variety of secondary metabolites with diverse biological activities, some of which have been developed for human medicine. rare actinomycetes are promising sources in search for new drugs, and their potential for producing biologically active molecules is poorly studied. in this work, we have investigated the diversity of actinomycetes in the shallow water sediments of the trondheim fjord (norway). due to the use of different selective isolation methods, an u ... | 2007 | 17922759 |
| [haloalkaliphilic actinomycetes in soils of mongolian desert steppes]. | the abundance of actinomycetes isolated from the soils of mongolian desert steppes varies from several thousand to hundreds of thousands of cfu/g soil, depending on soil type and isolation medium. eight actinomycete genera have been found in these soils: streptomyces, micromonospora, saccharopolyspora, actinomadura, microtetraspora, thermomonospora, nocardia, and dactylosporangium. the streptomycete complexes of brown desert-steppe and gray-brown desert alkaline soils include halophilic, alkalip ... | 2007 | 17966911 |
| elucidation of the kijanimicin gene cluster: insights into the biosynthesis of spirotetronate antibiotics and nitrosugars. | the antibiotic kijanimicin produced by the actinomycete actinomadura kijaniata has a broad spectrum of bioactivities as well as a number of interesting biosynthetic features. to understand the molecular basis for its formation and to develop a combinatorial biosynthetic system for this class of compounds, a 107.6 kb segment of the a. kijaniata chromosome containing the kijanimicin biosynthetic locus was identified, cloned, and sequenced. the complete pathway for the formation of tdp-l-digitoxose ... | 2007 | 17985890 |
| [echinomycin production by actinomadura sp. ina 654]. | an actinomycete strain designated as actinomadura sp. ina 654 was isolated from a chernozem soil sample in the voronezh region by the soil sample treatment with millimetric waves (ehf band). the strain produced an antibiotic complex of 2 components, named a-654-i and a-654-ii. investigation of their physico-chemical properties showed that a-654-i was identical to echinomycin, a heteropeptide lactone of the quinoxaline group with antitumor activity, while a-654-ii proved to be likely a new natura ... | 2006 | 18030784 |
| cloning, sequencing, and characterization of the pradimicin biosynthetic gene cluster of actinomadura hibisca p157-2. | pradimicins are potent antifungal antibiotics having an unusual dihydrobenzo[alpha]naphthacenequinone aglycone substituted with d-alanine and sugars. pradimicins are polyketide antibiotics produced by actinomadura hibisca p157-2. the gene cluster involved in the biosynthesis of pradimicins was cloned and sequenced. the pradimicin gene cluster was localized to a 39-kb dna segment and its involvement in the biosynthesis of pradimicin was proven by gene inactivation of prma and prmb (ketosynthases ... | 2007 | 18051306 |
| utility of helical computed tomography to evaluate the invasion of actinomycetoma; a report of 21 cases. | actinomycetoma is a chronic infection caused by several aerobic actinomycetes; it is a relatively frequent condition in tropical countries like mexico. it is important to be aware of the extension and depth of the disease (bone and visceral) to make the prognosis and select treatment. | 2008 | 18294320 |
| enzyme production by genetically engineered streptomyces strains: influence of culture conditions. | production of various extracellular enzymes (the beta-lactamases from streptomyces albus g, streptomyces cacaoi, actinomadura r39, and the dd-carboxypeptidase from streptomyces r61) by genetically engineered streptomyces lividans tk24 in lennox broth medium reached a maximum after 36 to 48 h. subsequently, the enzyme activity drastically decreased probably due to an increased ph value and the production of an inactivator by streptomyces lividans. protease activity did not seem to play a major ro ... | 1990 | 18592568 |
| actinomadura bangladeshensis sp. nov. and actinomadura chokoriensis sp. nov. | the taxonomic position of two soil isolates, 3-46-b(3)(t) and 3-45-a(11)(t), was clarified based on data from a polyphasic study. the organisms showed a combination of chemotaxonomic and morphological properties typical of the genus actinomadura. they formed distinct phyletic lines in the actinomadura 16s rrna gene tree and were closely associated with the type strains of actinomadura meyerae (sequence similarity of 98.3-98.5 %), actinomadura napierensis (98.1-98.3 %) and actinomadura latina (96 ... | 2008 | 18599711 |
| crystal structures of complexes of bacterial dd-peptidases with peptidoglycan-mimetic ligands: the substrate specificity puzzle. | the x-ray crystal structures of covalent complexes of the actinomadura r39 dd-peptidase and escherichia coli penicillin-binding protein (pbp) 5 with beta-lactams bearing peptidoglycan-mimetic side chains have been determined. the structure of the hydrolysis product of an analogous peptide bound noncovalently to the former enzyme has also been obtained. the r39 dd-peptidase structures reveal the presence of a specific binding site for the d-alpha-aminopimelyl side chain, characteristic of the ste ... | 2008 | 18602645 |
| rheologies and morphologies of three actinomycetes in submerged culture. | the broth rheologies and morphologies of three actinomycetes (saccharopolyspora erythraea, actinomadura roseorufa, and streptomyces rimosus) in submerged culture have been examined. the rheology of all the broths became pseudoplastic as soon as significant growth occurred with the power law index, n, falling to 0.20 to 0.25. the consistency index, k, rose with biomass concentration although in some instances it fell later in the fermentation. the mean main hyphal lengths of all cultures were in ... | 1995 | 18623054 |
| therapeutic effect of a novel oxazolidinone, da-7867, in balb/c mice infected with nocardia brasiliensis. | mycetoma is a chronic infectious disease of tropical and subtropical countries. it is produced by true fungi and actinobacteria. in méxico, nocardia brasiliensis is the main causative agent of mycetoma, producing about 86% of the cases; the gold standard for the therapy of mycetoma by n. brasiliensis is the use of sulfonamides which give a 70% cure rate. the addition of amikacin to this regime increases to 95% the cure rate; however, the patients have to be monitored for creatinine clearance and ... | 2008 | 18820738 |
| [diversity of actinomycetes in wuling mountain]. | to isolate new actinomycetes for discovering compounds of pharmaceutical importance. | 2008 | 18837376 |
| actinobacterial diversity from marine sediments collected in mexico. | seventeen different media known to support the growth and isolation of members of the class actinobacteria were evaluated as selective isolation media for the recovery of this microbial group from marine sediments samples collected in the gulf of california and the gulf of mexico. a general selective isolation procedure was employed for six sediments and nearly 300 actinomycetes were recovered from the selective isolation plates. full 16s rrna gene sequencing revealed that the isolates belonged ... | 2009 | 19023674 |
| actinomadura maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere soil of maheshkhali, bangladesh. | the taxonomic position of one soil isolate 13-12(50)(t) was clarified by a polyphasic study. the organism showed a combination of chemotaxonomic and morphological properties typical of the genus actinomadura. it formed a distinct phyletic line in the actinomadura 16s rrna gene tree and was closely associated with actinomadura mexicana (sequence similarity 99.5%), actinomadura glauciflava, actinomadura citrea (sequence similarity 99.4%) and actinomadura madurae (sequence similarity 99.2%). the re ... | 2008 | 19164876 |
| mutational analysis of the substrate specificity of escherichia coli penicillin binding protein 4. | escherichia coli pbp4 is the archetypal class c, low molecular mass penicillin binding protein (lmm-pbp) and possesses both dd-carboxypeptidase and dd-endopeptidase activity. in contrast to other classes of pbp, class c lmm-pbps show high dd-carboxypeptidase activity and rapidly hydrolyze synthetic fragments of peptidoglycan. the recently solved x-ray crystal structures of three class c lmm-pbps (e. coli pbp4, bacillus subtilis pbp4a, and actinomadura r39 dd-peptidase) have identified several re ... | 2009 | 19209901 |
| discovery of additional members of the tyrosine aminomutase enzyme family and the mutational analysis of cmdf. | the tyrosine aminomutase (tam) cmdf converts l-tyr preferentially to (r)-beta-tyr--a biosynthetic building block subsequently incorporated into the highly cytotoxic chondramides by the myxobacterium chondromyces crocatus. together with the similar enzymes sgcc4 from streptomyces globisporus and mdpc4 from actinomadura madurae, which preferentially produce (s)-beta-tyr, cmdf belongs to a novel 2,3-aminomutase enzyme family closely related to the aromatic amino acid ammonia lyase. although conside ... | 2009 | 19222035 |
| actinomadura miaoliensis sp. nov., a thermotolerant polyester-degrading actinomycete. | an actinomycete, strain bc 44t-5(t), with the ability to degrade poly(d-3-hydroxybutyrate) was isolated from a soil sample collected from miaoli county, taiwan. the isolate displayed substrate mycelia and short spore chains were borne on the aerial mycelia. spores were non-motile, round, 1 mum in diameter and spiny. the aerial spore mass was blue. strain bc 44t-5(t) had meso-diaminopimelic acid as the diagnostic diamino acid of the cell-wall peptidoglycan. whole-cell sugars of the novel strain w ... | 2009 | 19244432 |
| [actinomadura madurae mycetoma of the foot. report of one case]. | mycetoma is a chronic infection that affects skin, subcutaneous tissue and bone. its etiology can be mycotic or bacterial. it affects mainly the lower extremities of middle age men living in tropical climates. we report a 44-year-old male living in a template zone, consulting for swelling and pain in the left foot, lasting for 10 years. physical examination showed a swollen left foot with hyperpigmented skin and a few crusted papules. radiology showed an extensive bone involvement of the midfoot ... | 2008 | 19301776 |
| actinomadura keratinilytica sp. nov., a keratin-degrading actinobacterium isolated from bovine manure compost. | a novel keratinolytic actinobacterium, strain wcc-2265(t), was isolated from bovine hoof keratin 'baited' into composting bovine manure from southern alberta, canada, and subjected to phenotypic and genotypic characterization. strain wcc-2265(t) produced well-developed, non-fragmenting and extensively branched hyphae within substrates and aerial hyphae, from which spherical spores possessing spiny cell sheaths were produced in primarily flexuous or straight chains. the cell wall contained meso-d ... | 2009 | 19329615 |
| isolation and characterization of medically important aerobic actinomycetes in soil of iran (2006 - 2007). | the aerobic actinomycetes are a large group of soil-inhabiting bacteria that occur worldwide. some of them are the main cause of two important diseases, nocardiosis and actinomycetoma. to identify the prevalence and geographic distribution of aerobic actinomycetes in soil of qazvin province, a study was carried out during 2006-2007. in this study, the incidence and diversity of medically important aerobic actinomycetes was determined in 300 soil samples of different parts of qazvin. the suspensi ... | 2009 | 19440253 |
| transfer of actinomadura spadix nonomura and ohara 1971 to actinoallomurus spadix gen. nov., comb. nov., and description of actinoallomurus amamiensis sp. nov., actinoallomurus caesius sp. nov., actinoallomurus coprocola sp. nov., actinoallomurus fulvus sp. nov., actinoallomurus iriomotensis sp. nov., actinoallomurus luridus sp. nov., actinoallomurus purpureus sp. nov. and actinoallomurus yoronensis sp. nov. | ten actinomycete strains that form chains of spiral or looped spores were isolated from soil and dung samples in japan. they contained d- and l-lysine, meso-diaminopimelic acid (a2pm), d-glutamic acid and d- and l-alanine in the cell-wall peptidoglycan, madurose as a characteristic whole-cell sugar, mk-9(h6) and mk-9(h8) as the major isoprenoid quinones and iso-c16:0 as the major cellular fatty acid and showed genomic dna g+c contents of 69-74 mol%. phylogenetic analyses based on 16s rrna gene s ... | 2009 | 19567582 |
| bioweathering and biotransformation of granitic rock minerals by actinomycetes. | actinomycetes inhabiting granitic rocks at st. katherine, egypt were investigated for their bioweathering potential. actinomycete counts ranged between 174 and 360 colony forming units per gram. counts were positively correlated to rock porosity (r = 0.65) and negatively correlated to rock salinity (r = -0.56). sixty-six actinomycete isolates originating from rocks could be assigned into eight genera, with a high frequency of nocardioides and streptomyces. organic acids were produced by 97% of t ... | 2009 | 19590809 |
| actinomadura flavalba sp. nov., an endophytic actinomycete isolated from leaves of maytenus austroyunnanensis. | an actinomycete strain, designated yim 61435(t), was isolated from leaves of maytenus austroyunnanensis collected from a tropical rainforest in xishuangbanna, yunnan province, south-west china. the isolate produced aerial mycelium with long, curved to hooked spore chains. the chemotaxonomic characteristics of the isolate were consistent with its assignment to the genus actinomadura. phylogenetic analysis using 16s rrna gene sequences also indicated that this strain should be classified in the ge ... | 2009 | 19622664 |
| actinomadura sputi sp. nov., isolated from the sputum of a patient with pulmonary infection. | the taxonomic position of an actinomycete, strain immib l-889(t), isolated from the sputum of a 64-year-old man, was determined using a polyphasic taxonomic approach. the strain had chemical and morphological properties that were consistent with its classification in the genus actinomadura. it formed a distinct phyletic line in the 16s rrna gene tree of actinomadura and was most closely related to the type strain of actinomadura hallensis (98.4 % sequence similarity), but could be readily distin ... | 2010 | 19648340 |
| [selective isolation and diversity of acidophilic filamentous actinomycetes from acidic soils]. | to establish effective methods for selective isolation of acidophilic filamentous actinomycetes from acidic soils, and to investigate their genus and species diversity. | 2009 | 19673405 |
| a novel isoquinoline alkaloid, dd-carboxypeptidase inhibitor, with antibacterial activity isolated from streptomyces sp. 8812. part i: taxonomy, fermentation, isolation and biological activities. | a novel isoquinoline alkaloid of molecular formula c10h9no4, labeled js-1, was isolated from the culture broth of streptomyces sp. 8812. it was purified by acetone protein precipitation from the culture supernatant, followed by anion exchange and c18 rp hplc columns. js-1 is an inhibitor of exocellular dd-carboxypeptidases/transpeptidases (dd-peptidases) 64-575 ii from saccharopolyspora erythraea 64-575 ii, and r39 from actinomadura r39. js-1 exhibits activity against gram-negative bacteria, suc ... | 2009 | 19713994 |
| effect of propargyl bromide and 1,3-dichloropropene on microbial communities in an organically amended soil. | abstract in this study we investigated the response of microbial communities in unamended and manure-amended soil treated with the fumigants propargyl bromide (pbr) and 1,3-dichloropropene (1,3-d). the soil fumigants were applied at a rate of 10, 100, and 500 mg kg(-1). after treatment of the soils, the metabolic activity was assessed by monitoring the dehydrogenase activity (dha). pbr and 1,3-d initially inhibited the dha when applied at 500 mg kg(-1); however, after 8 weeks, recovery of the dh ... | 2003 | 19719698 |
| synthesis and evaluation of 3-(dihydroxyboryl)benzoic acids as d,d-carboxypeptidase r39 inhibitors. | penicillin binding proteins (pbps) catalyze steps in the biosynthesis of bacterial cell walls and are the targets for the beta-lactam antibiotics. non-beta-lactam based antibiotics that target pbps are of interest because bacteria have evolved resistance to the beta-lactam antibiotics. boronic acids have been developed as inhibitors of the mechanistically related serine beta-lactamases and serine proteases; however, they have not been explored extensively as pbp inhibitors. here we report aromat ... | 2009 | 19731939 |
| red grain botryomycosis due to staphylococcus aureus--a novel case report. | staphylococcus aureus and some other bacteria are known to cause botryomycosis. these bacteria are known to produce yellowish-white soft grains. only actinomadura pelletieri is known to produce red grains. we report here a case of an intensely inflammatory type of botryomycosis. a 35-year-old male labourer presented with swelling, redness and multiple sinuses on his foot, of eight months duration. the purulent discharge contained bright red coloured grains, 0.5 to 1 mm in size, which were round ... | 2009 | 19736413 |
| a case of actinomycotic mycetoma involving the right foot. | a 45-year-old male presented with history of multiple swellings over the foot with sinuses discharging seropurulent pus. actinomadura madurae was demonstrated and identified by microbiological culture from the pus obtained directly of the lesion. this case is reported to emphasize the importance of laboratory diagnosis in the management and assessment of the prognosis of such cases. | 2009 | 19749452 |
| structural basis of the inhibition of class a beta-lactamases and penicillin-binding proteins by 6-beta-iodopenicillanate. | 6-beta-halogenopenicillanates are powerful, irreversible inhibitors of various beta-lactamases and penicillin-binding proteins. upon acylation of these enzymes, the inhibitors are thought to undergo a structural rearrangement associated with the departure of the iodide and formation of a dihydrothiazine ring, but, to date, no structural evidence has proven this. 6-beta-iodopenicillanic acid (bip) is shown here to be an active antibiotic against various bacterial strains and an effective inhibito ... | 2009 | 19919161 |
| development and characterization of a transdermal patch and an emulgel containing kanamycin intended to be used in the treatment of mycetoma caused by actinomadura madurae. | mycetoma is a chronic, degenerative, and incapacitating infection of the skin and subcutaneous tissue. | 2009 | 19929211 |
| actinomadura scrupuli sp. nov., isolated from rock. | a novel actinomycete, designated strain r-ac121(t), was isolated from a small stone collected from an agricultural field in jeju, republic of korea. the organism formed abundant vegetative mycelium that was branched and twisted. the reverse colour of colonies was brownish-yellow. non-motile, wrinkled arthrospores were produced directly on the substrate mycelium. aerial mycelium and sporangia were not observed. a neighbour-joining tree based on 16s rrna gene sequences indicated that the isolate f ... | 2010 | 20042751 |
| a novel poly (l-lactide) degrading actinomycetes isolated from thai forest soil, phylogenic relationship and the enzyme characterization. | thirteen poly (l-lactide)-degrading microorganisms were isolated and selected based on their ability of clear zone formation on an emulsified pla agar plate and the enzyme activity in culture broth. according to phenotypic properties and 16s rrna gene sequence, these strains were classified to various families such as thermomonosporaceae, micromonosporaceae, streptosporangiaceae, bacillaceae and thermoactinomycetaceae. strain t16-1, identified as actinomadura sp., demonstrated the highest pla-de ... | 2009 | 20118610 |
| x-ray structure of kijd3, a key enzyme involved in the biosynthesis of d-kijanose. | d-kijanose is an unusual nitrosugar found attached to the antibiotic kijanimicin. ten enzymes are required for its production in actinomadura kijaniata, a soil-dwelling actinomycete. the focus of this investigation is on the protein encoded by the kijd3 gene and hereafter referred to as kijd3. on the basis of amino acid sequence analyses, kijd3 has been proposed to be an fad-dependent oxidoreductase, which catalyzes the sixth step in d-kijanose biosynthesis by converting dtdp-3-amino-2,3,6-tride ... | 2010 | 20334431 |
| [filamentous "contaminants" in the mycobacteriology laboratory; their culture, identification and clinical significance]. | frequent "contaminants" detected during mycobacterial culture of decontaminated samples are bacteria of the order actinomycetales. these are usually bacteria classified as the family corynebacterineae, genera corynebacterium, dietzia, gordonia, nocardia, rhodococcus and tsukamurella. these bacteria frequently colonize the airways and, under certain circumstances, they may cause life-threatening diseases. in severely immunocompromised patients, they regularly cause life-threatening infections wit ... | 2010 | 20503156 |
| jbir-65, a new diterpene, isolated from a sponge-derived actinomadura sp. spb081030sc-15. | | 2010 | 20551988 |
| crystal structure of a complex between the actinomadura r39 dd-peptidase and a peptidoglycan-mimetic boronate inhibitor: interpretation of a transition state analogue in terms of catalytic mechanism. | the actinomadura r39 dd-peptidase is a bacterial low molecular weight class c penicillin-binding protein. it has previously been shown to catalyze hydrolysis and aminolysis of small d-alanyl-d-alanine terminating peptides, especially those with a side chain that mimics the amino terminus of the stem peptide precursor to the bacterial cell wall. this paper describes the synthesis of (d-alpha-aminopimelylamino)-d-1-ethylboronic acid, designed to be a peptidoglycan-mimetic transition state analogue ... | 2010 | 20608745 |
| enediyne antitumor antibiotic maduropeptin biosynthesis featuring a c-methyltransferase that acts on a coa-tethered aromatic substrate. | the enediyne antitumor antibiotic maduropeptin (mdp) is produced by actinomadura madurae atcc 39144. the biosynthetic pathway for the 3,6-dimethylsalicylic acid moiety of the mdp chromophore is proposed to be comprised of four enzymes: mdpb, mdpb1, mdpb2, and mdpb3. based on the previously characterized biosynthesis of the naphthoic acid moiety of neocarzinostatin (ncs), we expected a biosynthetic pathway featuring carboxylic acid activation by the mdpb2 coa ligase immediately before its couplin ... | 2010 | 20718468 |
| [mycetoma in tunisia: a 15-case series]. | mycetoma is a chronic infection of cutaneous and subcutaneous tissue that can be caused by fungi or bacteria. it is endemic in tropical and subtropical areas but rare in tunisia. | 2010 | 20734597 |
| actinomadura apis sp. nov., isolated from a honey bee (apis mellifera) hive in thailand and the reclassification of actinomadura cremea subsp. rifamycini gauze et al. 1987 as actinomadura rifamycini (gauze et al. 1987) sp. nov., comb. nov. | a gram-positive aerobic actinomycete, designated strain im17-1(t), was isolated from a honey bee (apis mellifera) hive collected from chiang mai province, thailand. this strain formed a branched substrate mycelium and the mature aerial mycelium bore short chains of arthrospores with warty surfaces. the cell wall contained meso-2,6-diaminopimelic acid (type iii) and the whole cell sugars were fucose, galactose, glucose, madurose, mannose and ribose. the major isoprenoid quinone was hexahydrogenat ... | 2010 | 20952541 |
| madura foot caused by actinomadura madurae in a pregnant woman. | | 2010 | 20956671 |
| mycetoma in south india: retrospective analysis of 13 cases and description of two cases caused by unusual pathogens: neoscytalidium dimidiatum and aspergillus flavus. | mycetoma is a chronic suppurative and/or granulomatous inflammatory lesion of skin, subcutaneous tissue, fascia, and tendons caused by the traumatic inoculation of either fungal (eumycotic) or bacterial (actinomycotic) organisms present in the soil. the disease is characterized by triad of tumefaction, discharging sinuses, and grains. | 2010 | 20964650 |
| kinetics of reactions of the actinomadura r39 dd-peptidase with specific substrates. | the actinomadura r39 dd-peptidase catalyzes the hydrolysis and aminolysis of a number of small peptides and depsipeptides. details of its substrate specificity and the nature of its in vivo substrate are not, however, well understood. this paper describes the interactions of the r39 enzyme with two peptidoglycan-mimetic substrates 3-(d-cysteinyl)propanoyl-d-alanyl-d-alanine and 3-(d-cysteinyl)propanoyl-d-alanyl-d-thiolactate. a detailed study of the reactions of the former substrate, catalyzed b ... | 2010 | 21182324 |
| actinomadura pelletieri mycetoma--an atypical case with spine and abdominal wall involvement. | we describe a case of mycetoma caused by actinomadura pelletieri with simultaneous involvement of the spine, abdominal wall and retroperitoneal space in a man who had suffered from 'madura foot' 10 years earlier. the characteristics of this case were analysed and contextualized among those of other cases of mycetoma caused by other micro-organisms found through a review of the international literature. the rarity of the disease in industrialized countries and its possible atypical presentations ... | 2011 | 21252272 |
| [diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province]. | to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china. | 2010 | 21268889 |
| actinomadura meyerae osteitis following wound contamination with hay in a woman in france: a case report. | | 2011 | 21269428 |
| cloning of a thermostable xylanase from actinomadura sp. s14 and its expression in escherichia coli and pichia pastoris. | a thermophilic xylan-degrading actinomadura sp. s14 was isolated from compost in thailand. hemicellulase activities such as endo-1,4-β-xylanase, β-xylosidase and α-arabinofuranosidase were induced with xylan-containing agriculture wastes and oat spelt xylan. the gene encoding xylanase consisting of 687bp was cloned from actinomadura sp. s14. the deduced amino acid sequence contained a signal peptide of 41 amino acids and a probable mature xylanase of 188 amino acids. an open reading frame (xyns1 ... | 2011 | 21269876 |
| molecular analysis of bacterial communities in uranium ores and surrounding soils from banduhurang open cast uranium mine, india: a comparative study. | bacterial community structure of heavy metal rich- uranium ores and surrounding soils was explored using 16s rrna gene based clone library analysis and denaturing gradient gel electrophoresis (dgge) to provide baseline microbial diversity data on autochthonous communities. sequence analysis of major ribotypes and/or dgge bands revealed proteobacteria and acidobacteria as the two most frequently present bacterial phyla across the samples, although relative abundance of each phyla and identity of ... | 2011 | 21308598 |
| characterization and identification of pradimicin analogs from actinomadura hibisca using liquid chromatography-tandem mass spectrometry. | microbial cultures produce complex and potentially interesting mixtures of biosynthetic intermediates and derivatives of metabolites. these mixtures' reliable identification is important and so too is the development of techniques for their analysis. here, a simple and highly selective method of detecting the biosynthetic congeners involved in the pentangular polyphenol pradimicin (pr) pathway from actinomadura hibisca fermentation was developed. solid-phase extraction (spe) cleanup using an oas ... | 2011 | 21376331 |
| actinomadura meridiana sp. nov., isolated from oreum (small mountain). | a new actinomycete, designated strain dls-45t, was from a soil of rock surface collected from the peak of darangshi oreum (small mountain) in jeju, republic of korea. substrate mycelium was well developed but aerial mycelium was scant on most of the media. spherical to subspherical spores on the aerial mycelium were in chains that were arranged in hooks and their surfaces were warty. the combination of morphological and chemical features supported the classification in the genus actinomadura. th ... | 2011 | 21398504 |
| enhancement of pradimicin production in actinomadura hibisca p157-2 by metabolic engineering. | actinomadura hibisca p157-2 produces potent antifungal antibiotic pradimicins. to enhance pradimicin production, accase from streptomyces coelicolor and two regulatory genes metk1-sp and afsr-sp from streptomyces peuticus were overexpressed into a. hibisca using an integration vector pset152 under the control of the strong erme* promoter. the constructed plasmids pacc152, psam152, pafs152, psa152 and pasa152 were transformed into a. hibisca by the conjugal method. the recombinant strains a. hibi ... | 2011 | 21570270 |
| unexpected tricovalent binding mode of boronic acids within the active site of a penicillin binding protein. | boronic acids bearing appropriate side chains are good inhibitors of serine amidohydrolases. the boron usually adopts a tetrahedral conformation, bound to the nucleophilic serine of the active site and mimicking the transition state of the enzymatic reaction. we have solved the structures of complexes of a penicillin-binding protein, the dd-peptidase from actinomadura sp. r39, with four amidomethylboronic acids (2,6 dimethoxybenzamidomethylboronic acid, phenylacetamidomethylboronic acid, 2-chlor ... | 2011 | 21574608 |
| a combined structural and functional investigation of a c-3''-ketoreductase involved in the biosynthesis of dtdp-l-digitoxose. | l-digitoxose is an unusual dideoxysugar found attached to various pharmacologically active natural products including the antitumor antibiotic tetrocarcin a, and the antibiotics kijanimicin and jadomycin b. six enzymes are required for its production starting from glucose-1-phosphate. here we describe a combined structural and functional investigation of kijd10, an nadph-dependent c-3'' ketoreductase that catalyzes the third step of l-digitoxose biosynthesis in the african soil-dwelling bacteriu ... | 2011 | 21598943 |
| actinomadura sediminis sp. nov., a novel marine actinomycete isolated from mangrove sediment in little andaman, india. | in the study, the taxonomic position of the actinobacterium, strain yim m 10931(t), which was isolated from a mangrove sediment sample collected in dugong creek, little andaman, india, was determined by a polyphasic approach. this gram-positive, aerobic strain produced a branched substrate mycelium and aerial hyphae, which differentiated into short, hooked or spiral spore chains. the organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. the wh ... | 2011 | 21705447 |
| actinomadura rupiterrae sp. nov., isolated from cliff soil. | a novel actinomycete strain, designated cs5-ac15(t), was isolated from a soil sample collected from a cliff on mara island, jeju, republic of korea and subjected to polyphasic taxonomic analysis. the isolate produces well-developed, yellow-coloured substrate mycelia and white aerial mycelia that differentiate into straight or flexuous chains of smooth-surfaced spores. 16s rrna gene sequence analyses showed that the organism belonged to the family thermomonosporaceae and formed a tight cluster wi ... | 2011 | 21685258 |
| bendigoles d-f, bioactive sterols from the marine sponge-derived actinomadura sp. sbms009. | marine derived actinomycetes have become an important source of bioactive natural products. here we report the structure and bioactivity of the bendigoles d-f (1-3), 3-keto sterols isolated from the new marine sponge derived bacterium, actinomadura sp. sbms009. the isolation of these compounds was guided by a novel high-content screen for nf-+¦b and glucocorticoid receptor (gr) activity, and cytotoxicity assays. the structures of 1-3 were determined by detailed analysis of nmr, ms, and single cr ... | 2011 | 21684166 |
| Purification and Biochemical Characterization of a Highly Thermostable Xylanase from Actinomadura sp. Strain Cpt20 Isolated from Poultry Compost. | An extracellular thermostable xylanase from a newly isolated thermophilic Actinomadura sp. strain Cpt20 was purified and characterized. Based on matrix-assisted laser desorption-ionization time-of-flight mass spectrometry analysis, the purified enzyme is a monomer with a molecular mass of 20,110.13 Da. The 19 residue N-terminal sequence of the enzyme showed 84% homology with those of actinomycete endoxylanases. The optimum pH and temperature values for xylanase activity were pH 10 and 80 °C, res ... | 2011 | 22161140 |
| A key cytochrome P450 hydroxylase in pradimicin biosynthesis. | Pradimicins A-C (1-3) are a group of antifungal and antiviral polyketides from Actinomadura hibisca. The sugar moieties in pradimicins are required for their biological activities. Consequently, the 5-OH that is used for glycosylation plays a critical role in pradimicin biosynthesis. A cytochrome P450 monooxygenase gene, pdmJ, was amplified from the genomic DNA of A. hibisca and expressed in Escherichia coli BL21(DE3). PdmJ introduced a hydroxyl group to G-2A (4), a key pradimicin biosynthetic i ... | 2012 | 22082563 |
| substrate specificity of low-molecular mass bacterial dd-peptidases. | the bacterial dd-peptidases or penicillin-binding proteins (pbps) catalyze the formation and regulation of cross-links in peptidoglycan biosynthesis. they are classified into two groups, the high-molecular mass (hmm) and low-molecular mass (lmm) enzymes. the latter group, which is subdivided into classes a-c (lmma, -b, and -c, respectively), is believed to catalyze dd-carboxypeptidase and endopeptidase reactions in vivo. to date, the specificity of their reactions with particular elements of pep ... | 2011 | 22029692 |
| Actinomadura geliboluensis sp. nov., isolated from soil in Canakkale, Turkey. | A novel actinobacterium strain, A8036T, isolated from soil, was determined using polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generate spiral chains spores with irregular-surfaces. The cell wall contained meso-diaminopimelic acid (type III) and cell wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9 (H6) and MK-9 (H4). The phospholipids were diphosphatidyl glycerol, phosphatidyl inositol and phosphatidyl ... | 2011 | 22021584 |