| [isolation and separation of nucleic acids from streptomyces hydrogenans (author's transl)]. | different methods for homogenization of cells of streptomyces hydrogenans, for extraction of nucleic acids and for fractionation of the rna and dna obtained were critically examined. the only way to prepare high molecular weight rapidly labelled rna and polysomes was to grind freeze-dried cells together with kieselguhr with a mortar and pestle. the best results for extraction of nucleic acids from the cell homogenate were obtained in the presence of diethyl pyrocarbonate (diethyl oxydiformate), ... | 1975 | 171211 |
| the functioning of a nicotinamide--adenine dinucleotide-dependent dehydrogenase and the structure adjacent to the reacting carbon atom of the substrate. | | 1975 | 172381 |
| septamycin, a polyether antibiotic. taxonomy, fermentation, isolation and characterization. | septamycin is a metal complexing polyether antibiotic produced by a strain of streptomyces hygroscopicus nrrl 5678. the metabolite, a monocarboxylic acid, was isolated as the sodium salt c48h81nao16. the crystal structure and absolute configuration were established by x-ray analysis of the p-bromophenacyl derivative. septamycin has a thirty-carbon backbone and contains seven heterocyclic rings. supported by direct comparison septamycin proved to be identical with antibiotic a28695 a isolated fro ... | 1975 | 172482 |
| effect of desdanine on nucleoside diphosphate kinase and pyruvate kinase of escherichia coli. | kinetic analysis demonstrated that the irreversible inhibition of nucleoside diphosphate kinase of escherichia coli by desdanine proceeds via a reversible enzyme-inhibitor complex. it is known that pyruvate kinase of e. coli becomes inactive upon prolonged dialysis in the absence of a reducing reagent, such as dithiothreitol and that the inactive enzyme is reactivated if dithiothretiol is added. desdanine inhibits this reactivation process. the effect is discussed in relation to inhibition in re ... | 1975 | 173700 |
| receptor for 5alpha-dihydrotestosterone from streptomyces hydrogenans. | streptomyces hydrogenans exhibits binding activity for 5alpha-dihydrotestosterone (17 beta-hydroxy-5alpha-androstan-3-one) in vivo. a cell free homogenate from the microorganism binds 5alpha-dihydrotestosterone in vitro as well. the association constant is 10(8) m-1 at 4 degrees c. progesterone, but neither testerone nor cyproterone, competes with 5alpha-dihydrotestosterone for binding. the binding fraction from streptomyces hydrogenans permits a simple assay for 5 alpha-dihydrotestosterone in b ... | 1975 | 175617 |
| [studies of tryptophan metabolism of streptomycetes. iv. studies of the inhibition of enzyme of tryptophan metabolism]. | | 1975 | 176831 |
| 17beta-hydroxysteroid dehydrogenase activity in streptomyces hydrogenans. | streptomyces hydrogenans converts 17beta-hydroxyandrost-4-ene-3-one (testosterone) to androst-4-ene-3,17-dione (androstenedione) in good yields. time-dependence of the conversion, steroid uptake and release have been studied in vivo. steroid analysis was done by thin-layer chromatography and recrystallization to constant specific radioactivity. after sonification of the cells the postulated 17beta-hydroxysteroid dehydrogenase activity was recovered in the 105 000 g supernatant. the enzyme was en ... | 1975 | 179288 |
| [the possible role of high molecular weight polyphosphates in chlortetracycline biosynthesis by streptomyces aureofaciens]. | the content of condensed inorganic polyphosphates in high productive and low productive streptomyces aureofaciens strains has been determined. at all the stages of growing the quantity of these compounds in low productive strain is 8-10-fold higher than in high productive strains. maximum accumulation of condensed inorganic polyphosphates in both the strains str. aureofaciens corresponds to the end of logarithmic phase of growth. in both strains the presence of two enzymes of polyphosphate biosy ... | 1976 | 179612 |
| classification of micrococci on the basis of deoxyribonucleic acid homology. | the dna homology relationships of 25 micrococci (15 strains of micrococcus, eight strains of sarcina and two strains of staphylococcus) were studied by the deoxyribonucleic acid hybridization method using nuclease s1, an endonuclease specific for single-stranded dna molecules. nineteen of the strains were classified into three groups. group i contained micrococcus lysodeikticus iami056, m. luteus iami1010, m. flavus iami2005 and iami2006, sarcina flava iami2007 and iami1006. s. subflava iami2009 ... | 1976 | 180238 |
| [mutagenic action of n-nitrosodimethylurea on actinomyces rimosus and penicillium chrysogenum]. | high mutagenic activity of n-nitrozodimethylurea (ndmu), an agent of the group of the nitrozo compounds not studied in detail was shown with respect to prototrophic and auxotrophic strains of actinomyces rimosus, an organism producing oxytetracycline and penicillium chrysogenum, an organism producing penicillin. the rate of direct and back mutations in the auxotrophic strain of act. rimosus under the effect of ndmu was many times higher than that of spontaneous mutations. ndmu was used at one of ... | 1976 | 182067 |
| vancomycin. | | 1976 | 183412 |
| purification and properties of guanosine 5', 3'-polyphosphate synthetase from bacillus brevis. | a ribosome-independent guanosine 5',3'-polyphosphate synthetase has been highly purified from bacillus brevis (atcc 8185). the enzyme has a molecular weight of 55,000, as measured by sucrose density gradient centrifugation. like the ribosome-connected stringent factor of escherichia coli, it catalyzes the synthesis of the guanosine 5', 3'-polyphosphates by a pyrophosphoryl transfer mechanism from adenosine triphosphate (atp) to guanosine di- or triphosphates (gdp, gtp). it has an apparent km of ... | 1976 | 184817 |
| a morphological and genetic mapping study of bald colony mutants of streptomyces coelicolor. | twelve bld mutations of streptomyces coelicolor resulting in a lack of visible aerial mycelium were mapped genetically. the mutants were classified into three groups on the basis of colony morphology, production of antibiotics and morphology on different carbon sources. four map locations were found for the bld genes and three of these were very near the loci of whi genes, which are also involved in differentiation. closely linked bld mutations had similar phenotypes. | 1976 | 186556 |
| [effect of actinomyces rimosus ribonuclease on the reproduction of viruses]. | antiviral activity of rna-ase isolated from the fermentation broth of actinomyces rimosus was studied. the effect of the enzyme on multiplication of the viruses of vesicular stomatitis, newcastle and cariolovaccine diseases was investigated. it was found that the enzyme was capable of suppressing reproduction of the vesicular stomatitis virus (vsv) in the culture of chick fibroblast cells. the suppression level directly depended on the enzyme concentration and decreased with an increase in the i ... | 1977 | 190944 |
| proteolytic activity of subcellular fractions from streptomyces griseus no. 45-h. | subcellular fractions were prepared from streptomyces griseus no. 45-h at different stages of life cycle, and their proteolytic activity was examined. the highest proteolytic activity was found in the 24- and 72- h-old vegetative hyphae, the lowest in the resting spores. spores contained about 9--30% of the proteolytic activity of vegetative cells. at the age of 16 h about 80%, at 26 h 70%, at 72 h 40%, and in spores about 60% of the proteolytic activity was particulate. the greatest part of the ... | 1976 | 193328 |
| mannitol oxidation in two micromonospora isolates and in representative species of other actinomycetes. | mannitol kinase and mannitol-1-phosphate dehydrogenase activities were detected in two micromonospora isolates. the presence of these enzyme activities indicates that mannitol is catabolized first to mannitol-1-phosphate and then to fructose-6-phosphate. mannitol-oxidizing enzymes were also surveyed in representative species of four other genera of actinomycetes. mannitol-1-phosphate dehydrogenase was detected in cell-free extracts of streptomyces lactamdurans. in contrast, cell-free extracts of ... | 1977 | 194534 |
| [features of secondary metabolism in microorganisms]. | | 1977 | 194631 |
| the substrate specificities of endo-beta-n-acetylglucosaminidases cii and h. | | 1977 | 199169 |
| effect of exogenous nucleotides on the candicidin fermentation. | addition of cyclic-amp (c-amp) to streptomyces griseus fermentations inhibited candicidin formation. in a phosphate-free resting cell system, c-amp inhibited net candicidin formation and incorporation of labeled propionate and p-aminobenzoic acid into the antibiotic but did not inhibit protein synthesis. all nucleotides tested, regardless of the position of the phosphate ester, were effective inhibitors; nucleosides and free bases were not. inhibition occurred whether the nucleotide was added ea ... | 1977 | 200324 |
| [biosynthesis and biology of nucleoside polyphosphate (author's transl)]. | | 1977 | 200684 |
| effect of dibutyryl cyclic adenosine monophosphate on active amino acid transport in streptomyces hydrogenans. | the active uptake of 2-aminoisobutyric acid (aib) and several other amino acids in resting cells of streptomyces hydrogenans was found to be stimulated by exogenously added adenosine cyclic monophosphate (camp). the uptake of glycerol, sorbose, and pyrimidine nucleosides remained unaffected. among the various camp derivatives tested, the dibutyryl derivative was found to be most effective, followed by monobutyryl camp, and camp. dibutyryl cgmp was also found to stimulate aib transport, and its e ... | 1977 | 202211 |
| beta-lactam antibiotics and related substances. | | 1977 | 206739 |
| intracellular cyclic adenosine 3',5'-monophosphate levels and streptomycin production in cultures of streptomyces griseus. | changes in the amount of cyclic 3',5'-adenosine monophosphate within the mycelium of streptomyces griseus were measured as cultures progressed through trophophase and idiophase in a complex medium supporting growth and streptomycin synthesis. concentrations were highest before the cultures entered stationary phase and had declined 90% by 5 h before the antibiotic was produced. this low conentration was maintained while the antibiotic accumulated during the idiophase. the results indicate that th ... | 1978 | 210919 |
| [use of the electron paramagnetic resonance method for the comparative evaluation of the effectiveness of the protective media used in the lyophilization of actimomycetes]. | the accumulation of free radicals correlates with an increase in the percentage of killed lyophilized spores of actinomycetes. therefore, the epr method in combination with the method of "accelerated storage" at 37 degrees c was used to evaluate rapidly (during one month) the effectiveness and to select certain protective media for stabilization of actinomycetes cultures. the epr method can be applied for comparative evaluation of only those media which did not contain originally free radicals. | 1978 | 212671 |
| atp and adenylate energy charge during phosphate-mediated control of antibiotic synthesis. | | 1978 | 213073 |
| modeling the role of cyclic amp in catabolite repression of inducible enzyme biosynthesis in microbial cells. | modeling the role of cyclic amp (camp) in catabolite repression of inducible enzyme production in microbial cells was studied. a catabolite repression index, f, was defined based on the postulation that complex formation occurs between rna polymerase (rnap) and dna, and shifting from the inert form to the open form of this complex (the latter form is required for transcription) is accelerated by the camp.crp complex. the catabolite repression index, f, was incorporated into model equations of mr ... | 1978 | 213139 |
| chemical modification of peptide antibiotics : part vii--biological activity of derivatives of polymyxin b. | | 1978 | 214395 |
| physical mapping of bk virus dna with saci, mboii, and alui restriction endonucleases. | a new restriction endonuclease, saci from streptomyces achromogenes cleaves bk virus (strain mm) dna into 3 fragments, whereas mboii from moraxella bovis and alui from arthrobacter luteus give 22 and 30 fragments, respectively. all these specific dna fragments were ordered and mapped on the viral genome by two methods first, by the reciprocal digestion method using uniformly 32p-labeled dna; and second, by the partial digestion technique using the single-end 32p-labeled dna. this study, together ... | 1978 | 215783 |
| [experimental study of the action of the rnase from actinomyces rimosus on the variolovaccine virus]. | the effect of actinomyces rimosus rnaase on the variolovaccine virus was studied. the inhibitory effect of the actinomyces rimosus rnaase on the variolovaccine virus reproduction in the tissue culture cells was shown. in the experiments with the use of chick embryons and rabbits this effect was less pronounced. a 10 time increase in the infection multiplicity both in the tissue culture and the chick embryons had no noticeable effect on the level of the virus inhibition. it was supposed that the ... | 1979 | 220906 |
| phase i and preliminary phase ii study of neocarzinostatin. | neocarzinostatin is a protein antitumor antibiotic isolated from cultures of streptomyces carzinostaticus var.f41. the drug has undergone extensive clinical trial in japan, and has been reported active against a variety of human tumors. a phase i and preliminary phase ii evaluation of the drug has been performed, using an iv bolus daily x 5 schedule. ninety-six patients have been treated at doses from 500 to 2250 units/m2/day. courses were repeated at 4-week intervals if allowed by bone marrow r ... | 1978 | 221112 |
| pantomycin: a new antimicrobial antibiotic. | | 1979 | 223049 |
| phosphate inhibition of secondary metabolism in streptomyces hygroscopicus and its reversal by cyclic amp. | inorganic phosphate inhibited the biosynthesis of the macrolide antibiotic turimycin in different strains of streptomyces hygroscopicus. in the wild type strain a depression was observed with increasing phosphate concentrations. a total inhibition was found at 0.1 m phosphate. in a high producing mutant a minimum of turimycin production occured when the phosphate concentration was between 5 mm and 10 mm. above this concentration the antibiotic synthesis increased again but the production period ... | 1979 | 223512 |
| inverse regulation of spore germination and growth by cyclic amp in streptomyces hygroscopicus. | the cyclic amp level in germinating spores of streptomyces hygroscopicus rises to a miximum ag outgrowth of germ tubes. exogenous cyclic amp results in an inverse effect on germination speed and growth. | 1979 | 223874 |
| streptomycin resistance in a streptomycin-producing microorganism. | cell-free extracts of streptomyces bikiniensis contain an adenosine 5'-triphosphate-dependent kinase which inactivates streptomycin (sm) and dihydrostreptomycin by phosphorylation. the products have been identified as streptomycin 6-phosphate and dihydrostreptomycin 6-phosphate. activity was not present in logarithmic-phase cells, which were susceptible to 25 mug of sm per ml. in stationary-phase cells, activity appeared 12 h before detectable sm in the medium. these cells were resistant to more ... | 1979 | 225985 |
| restriction enzyme map for streptomycete plasmid puc3. | | 1979 | 231272 |
| regulative influence of o-aminobenzoic acid on the biosynthesis of nourseothricin in cultures of streptomyces noursei ja 3890b. iv. bistability of metabolism and the mechanism of action of aminobenzoic acids. | using the semi-continuous cultivation technique we could establish that specifically in streptomyces noursei ja 3890b during growth on a medium supplied with d,l-alanine, nh4+, and maize starch there are two different phenotypes of the organism and stationary states of metabolism, respectively. the expression of either the metabolic state i with an enhanced capacity to oxidative deamination of alanine via the nad+-dependent alanaine dehydrogenase or the metabolic state 2 which may be characteriz ... | 1979 | 231865 |
| studies on the substrate specificity of the dna methylase activity from escherichia coli k-12. | a partially purified extract of dna methylases from e. coli k-12 containing dna-adenine as well as dna-cytosine methylase activities has been examined with respect to different dna species as substrates. the results show that the natural content of 6-map) in the applied dna represses the dna-adenine methylase activity. on the other hand, 5-mc, already present in the substrate does not influence the activity of the dna-cytosine methylase. dna from micrococcus radiodurans, which is completely free ... | 1979 | 232591 |
| the specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro. | all the eight 5'-di(tri)-3'-diphosphates of four common ribonucleosides were prepared by the enzymic pyrophosphoryl transfer catalysed by streptomyces adephospholyticus atp:nucleotide pyrophosphokinase (e.c.2.7.6.4) form datp to the respective 5'-phosphates, and their effects on the translation of mrnas by a wheat germ system in vitro were studied. (p) pppupp decreased the total 14c-leucine incorporation directed by a rat liver mrna whereas (p) pppypp did not. with a silkworm pupa ovary mrna, di ... | 1979 | 232758 |
| detection and changes of nucleotide pyrophosphotransferase activity of streptomyces griseus strains in submerged culture. | nucleotide pyrophosphotransferase (npt) activity of two streptomyces griseus strains was studied in submerged culture during their life cycle. npt activity could be detected only in the culture filtrate but not in the membrane fraction or in cell extract of the sporulating (streptomycin-non-producing) s. griseus no. 45-h. no enzyme could be detected in the non-sporulating (streptomycin-producing) s. griseus no 52--1 cultures. | 1979 | 233058 |
| detection and changes of nucleotide pyrophosphotransferase activity in cultures of streptomyces griseus strains on solid media. | the occurrence of nucleotide pyrophosphotransferase (atp: nucleotide 5'-phosphate pyrophosphotransferase, ec 2.7.4) and the changes in its activity were studied during differentiation in cultures of two streptomyces griseus strains grown on the surface of a solid medium. one of the strains does not sporulate and produce nucleotide pyro photransferase in submerged culture. nucleotide pyrophosphotransferase could be detected, though in much differing amounts, in surface cultures of both strains. | 1979 | 233457 |
| biosynthesis of streptothricin antibiotics. iv. on the incorporation of l-arginine into streptolidine moiety by streptomyces lavendulae op-2. | | 1977 | 233749 |
| hormonal involvement in thermal acclimation in the fiddler crab uca pugilator (bosc). ii. effects of extracts on tissue respiration. | | 1975 | 234333 |
| powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. | agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to ph 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. more actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and gave results comparable to those obtained with media prepared from colloidal suspensions. | 1975 | 234719 |
| enzymic and physicochemical properties of streptomyces griseus trypsin. | streptomyces griseus trypsin has been isolated from pronase by ion-exchange chromatography on cm-sephadex and se-sephadex. the isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. the latter could be partially resolved by chromatography on bio-rex 70. the molar absorbancy at 280 nm was found to be 3.96 times 10-4 m-1/cm and the e1cm1% was found to be 17.3. the molecular weight was 22,800 plus or minus 800. the enzy ... | 1975 | 235280 |
| rsistance to streptomycin in a producing strain of streptomyces griseus. | streptomyces griseus s 104 was sensitive to streptomycin during exponential growth in a medium which, in the subsequent stationary phase, supported production of the antibiotic in yields above 200 mug/ml. when antibiotic production began cultures developed a tolerance toward their lethal metabolite. this was not due to an increase in ph associated with antibiotic production, since ph effects on streptomycin sensitivity in s. griseus were in the reverse direction. however, the degree of tolerance ... | 1975 | 235356 |
| purification and characterization of an endonuclease from calf thymus acting on irradiated dna. | an endonuclease acting on dna exposed to ultraviolet light or gamma-rays has been extensively purified from calf thymus. the enzyme has a ph optimum at ph 7.0-7.5, acts with equal efficiency in the presence of edta or divalent cations (mg-2+ or ca-2+), is inhibited by nacl and trna and is inactivated by incubation at 50 degrees c. its molecular weight, determined by sephadex chromatography or sodium dodecylsulfate gel electrophoresis, is approx. 30 000. the enzyme catalyzes the formation of brea ... | 1975 | 236041 |
| degradation of feather keratin by culture filtrates of streptomyces fradiae. | treatment of milled defatted capon feathers with filtrates of steptomyces fradiae atcc 10745 that had been concentrated 20 times showed that the most keratin degradation in 0.5h occurred at ph 8.0 and 60degrees c. if assay time was extended, however, adecrease in reaction rate occurred and appeared to result from keratinase instability at elevated temperatures. exposure of commercial feather meal to unconcentrated culturefiltrates for 15 h produced greatest degradation at 50 degrees c. | 1975 | 236085 |
| a new substrate for investigating the specificity of beta-glucan hydrolases. | | 1975 | 236912 |
| strain development. | | 1975 | 237210 |
| novobiocic acid synthetase. | | 1975 | 237214 |
| s-adenosylmethionine:o-demethylpuromycin o-methyltransferase. | | 1975 | 237215 |
| mannosidostreptomycin hydrolase. | | 1975 | 237218 |
| penicillin acylase (bacterial). | | 1975 | 237220 |
| toyocamycin nitrile hydrolase. | | 1975 | 237224 |
| [isolation of surface antigens of influenza virus a/hong kong 1/68 (h3n2) by density gradient fractionation with a new carboxypolypeptidase (author's transl)]. | isolation of pure neuraminidase and hemagglutinin from influenza virus a/hong kong 1/68 (h3n2) can be achieved using the coupled action of a new carboxypolypeptidase from streptomyces fradiae and sodium dodecyl sulfate (sds). biochemical, physical and physico-chemical studies showed that the two molecules are in pure form and of glycoproteic nature. | 1975 | 238492 |
| two enzymes in streptomyces griseus forb the synthesis of dtdp-l-dihydrostreptose from dtdp-6-deoxy-d-xylo-4-hexosulose. | | 1975 | 238519 |
| studies on the interaction between streptomyces pepsin inhibitor and several acid proteinases by means of a zinc(ii)-dye complex as a probe. | the zinc(ii) complex of pyridine-2-azo-p-dimethylaniline is bound to several acid proteinases, at ph 5.0, accompanied by a change is the visible absorption spectrum. streptomyces pepsin inhibitor, which was discovered by satoi and murao (satoi, s. and murao, s. (1970) agric. biol. chem. 34, 1265-1267 and satoi, s. and murao, s. (1971) agric. biol. chem. 35, 1482-1487), is also bound to acid proteinases. spectrophotometric studies with ten acid proteinases from different sources have revealed tha ... | 1975 | 238604 |
| the microbial colonization of some woods of small dimensions buried in soil. | several species of wood veneer, including some in a green undried state, were buried in various soils, and at intervals the colonists were isolated and identified. in addition, veneers were deteriorated for different periods of time, sterilized, and then reburied in the same soil. isolates were obtained before sterilization and compared with those found afterwards. in each case the colonization involved a small number of microfungi and, because similar species were repeatedly isolated, an absenc ... | 1975 | 238728 |
| affinity chromatography of alpha-chymotrypsin, subtilism, and metalloendopeptidases on carbobenzoxy-l-phenylalanyl-triehtylenetetraminyl-sepharose. | carbobenzoxy-l-phenylalanyl-triethylenetetraminyl-sepharose (z-l-phe-t-sepharose) was found to be an effective affinity adsorbent for bovine pancreatic alpha-chymotrypsin [ec 3.4.21.1] as well as neutral [ec 3.4.24.4] and alkaline [ec 3.4.21.14] proteases of bacillus species. these enzymes were adsorbed in the neutral ph range. alpha-chymotrypsin was recovered by elution with 0.1 a acetic acid while neutral subtilopeptidase was eluted with 0.5 m nacl at ph 0. thermolysin and subtilisin were foun ... | 1975 | 238966 |
| properties of d-xylose isomerase from streptomyces albus. | a partially purified d-xylose isomerase has been isolated from cells of streptomyces albus nrrl 5778 and some of its properties have been determined. d-glucose, d-xylose, d-ribose, l-arabinose, and l-rhamnose served as substrates for the enzyme with respective km values of 86, 93, 350, 153, and 312 mm and vmax values measuring 1.23, 2.9, 2.63, 0.153, and 0.048 mumol min per mg of protein. the hexose d-allose was also isomerized. the enzyme was strongly activated by 1.0 mm mg2+ but only partially ... | 1975 | 239628 |
| spider mite webbing. iii. solubilization and amino acid composition of the silk protein. | | 1975 | 239809 |
| purification and characterization of a streptomyces albus endo-n-acetylmuramidase lytic for group a and other beta haemolytic streptococci. | the purification and characterization of the streptolytic exo-enzyme from the maxted-mccarty strain of streptomyces albus is described. this enzyme was shown to be an endo-n-acetylmuramidase with a molecular weight of 10 to 12,000 and optimal activity at ph 8 and 45 degrees c. the enzyme is lytic for streptococci of various groups, micrococcus lysodeikticus, staphylococcus aureus, as well as escherichia coli. it closely resembles the f1 endo-n-acetylmuramidase described by ghuysen et al. (1966) ... | 1975 | 240102 |
| specific inhibition of endo-beta-n-acetylglucosaminidase d by mannose and simple mannosides. | | 1975 | 240364 |
| purification and properties of an anti-b hemagglutinin produced by streptomyces sp. | an anti-b hemagglutinin was purified to homogeneity from the culture filtrate of a strain of streptomyces sp. by affinity chromatography. the streptomyces hemagglutinin was adsorbed to insolubilized gum arabic and eluted with 1 m nacl containing 1 m d-galactose. the purified hemagglutinin is thought to be homogeneous judging from sodium dodecyl sulfate-polyacrylamide gel electrophoresis at ph 7.2, disc gel electrophoresis at ph 4.3, isoelectric focusing, and ultracentrifugation. the molecular we ... | 1975 | 240402 |
| the catalytic activity and penicillin sensitivity in the liquid and frozen states of membrane-bound and detergent-solubilised transpeptidase of streptomyces r61. | the km, app. values of the membrane-bound transpeptidase of streptomyces r61 for the donor ac2-l-lys-d-ala-d-ala and the acceptor gly-gly are not affected by temperature variations when the reaction mixtures are incubated in liquid suspensions. at -5 degrees c, the incubation can be carried out either in the liquid or in the frozen state. the enzyme is active in the latter state. in the frozen state, the km, app. value for the acceptor remains unchanged but there is a 3-fold increase in the maxi ... | 1975 | 240708 |
| regulation of biosynthesis of secondary metabolites. xvii. purification and properties of malate dehydrogenase (decarboxylating) in streptomyces aureofaciens. | the process of isolation and purification of malate dehydrogenase (decarboxylating) (ec 1.1.1.40) from the mycelium of the actinomycete streptomyces aureofaciens has been worked out. the enzyme was purified 35 fold. the kinetic characters of the purified enzyme are very similar to the figures for malate dehydrogenase (decarboxylating) from other sources. km for l-malate = 2.1 x 10(-3)m, km for nadp = 4.6 x 10(-5)m (at ph 7.4). the reaction requires metal divalent ions, mn2+ being more effective ... | 1975 | 240762 |
| [precursor formation and biosynthesis of the macrolide antibiotic a 6599 (turimycin) by streptomyces hygroscopicus ja 6599]. | the possible role of some metabolic systems producing acetyl-coa, and methylmalonyl-coa as initial precursors in the biosynthesis of the macrolide antibiotic a 6599 by streptomyces hygroscopicus ja 6599 was studied. the activities of pyruvate decarboxylase exceeded in two higher producing strains about twofold those found in the mycelium of a lower producing one suggesting that in this organism an enhanced production of acetyl-coa should be one of the prerequisites necessary for an improved anti ... | 1975 | 241159 |
| streptovirudins -- new antibiotics with antiviral activity. the antiviral spectrum and inhibition of newcastle disease virus in cell cultures. | streptovirudins are new antibiotics isolated as a mixture of several structurally related compounds from fermentations of streptomyces griseoflavus (krainsky) waksman et henrici var. thuringensis ja 10124. they possess antiviral activity against rna and dna viruses cultivated in chick embryo cells, namely sindbis, fowl plague, newcastle disease (ndv), pseudorabies, vaccinia and sheep abortion viruses. the naturally formed streptovirudin complex, in concentrations of 20-2.5 mug/ml inhibited the v ... | 1975 | 241229 |
| analysis of oligomannosyl cores of cellular glycopeptides by digestion with endo-beta-n-acetylglucosaminidases. | | 1975 | 241347 |
| mutational biosynthesis of a new antibiotic, streptomutin a, by an idiotroph of streptomyces griseus. | microorganisms producing antibiotics have been genetically converted by earlier workers to mutants which cannot produce antibiotic without supplementation with a moiety of the antibiotic. these antibiotics include neomycin, kanamycin, paromomycin, butirosin, sisomicin, ribostamycin and novobiocin. success has not been reported for organisms producing guanidinocyclitol antibiotics such as streptomycin. we mutagenized conidia of the streptomycin-producing streptomyces griseus strain 7-455f3 with n ... | 1975 | 241738 |
| production and property of beta-lactamases in streptomyces. | the production of beta-lactamases by 100 strains of streptomyces was studied. about one-half of the strains produced more than 2.3 u of beta-lactamase per ml, and another half produced less than 1.4 u/ml. the amounts of beta-lactamases produced by two strains were in the order of those produced by bacillus cereus 569/h and bacillus licheniformis 749/c. these streptomyces enzymes function primarily as penicillinases rather than cephalosporinases. properties such as ph optimum, substrate specifici ... | 1975 | 242252 |
| effects of the gastric juice inhibitory substance from streptomyces bottropensis on gastric secretion and experimental ulcerations in rats. | | 1977 | 264182 |
| [effects of a bacteriolytic enzyme from streptomyces globisporus on microflora of human dental plaque and tongue coating]. | | 1977 | 275313 |
| lysis of streptococcus mutans cells with mutanolysin, a lytic enzyme prepared from a culture liquor of streptomyces globisporus 1829. | | 1978 | 281197 |
| degradation of abnormal proteins in intact mouse reticulocytes: accumulation of intermediates in the presence of bestatin. | incubation of intact mouse reticulocytes with bestatin (a competitive inhibitor of aminopeptidases) produced the accumulation of low molecular weight intermediates in the degradation of puromycinyl-peptides or analog-containing proteins that had been pulse labeled with l-[1-14c]leucine. a large fraction of the radioactive protein was degraded to trichloroacetic acid-soluble products within 10 min. in the presence of bestatin (0.5 mg/ml), one-fourth of these products appeared to be dipeptides, tr ... | 1979 | 284394 |
| patenting certain forms of life: a moral justification. | | 1979 | 289640 |
| topological organization of proteins in an intracellular secretory organelle: the synaptic vesicle. | intact synaptic vesicles prepared from the electric organ of the marine elasmobranch narcine brasiliensis have eight major polypeptides demonstrable on sodium dodecyl sulfate gels. six of these copurify with the synaptic vesicles during isolation of vesicles by chromatography on cpg-3000 and, by this criterion, are specific to vesicles. the other two are either shared by many membrane or are contaminants. one of these proteins comigrates with actin. three different approaches were used to determ ... | 1979 | 291069 |
| [determination of streptomyces coelicolor a3(2) resistance to erythromycin]. | resistance to erythromycin is genetically unstable in strains of streptomyces coelicolor a3(2). the frequent loss of resistance as well as reversion of sensitive variants to the original unstable resistance phenotype excluded the possibility that plasmid elimination is involved. the spontaneous frequency of occurrence of sensitive clones was 0.14 to 1.5%, the rate of reversion ranging from 1.10(-6) to 1.10(-8). resistance to erythromycin has been mapped on the chromosomes of two s. coelicolor a3 ... | 1979 | 291565 |
| a possible naturally occurring tumor promoter, teleocidin b from streptomyces. | | 1979 | 292437 |
| [genetic characteristics and genome structure of streptomyces coelicolor actinophages]. | actinophage phi c31 of streptomyces coelicolor a3 (2) and two novel temperate actinophages phi c43 and phi c62 isolated from strains of blue actinomycetes group are homoimmune, serologically and functionally related. dna molecules of phages phi c31, phi c43 and phi c62 have cohesive ends; sizes of dnas of these phages and some mutants have been determined. the extent of homology between the dnas of three phages is 93-96% as shown by heteroduplex analysis. the regions of non-homology are of a del ... | 1979 | 292617 |
| [effects of sodium n-lauroyl sarcosinate and sodium lauryl sulfate on the activity of bacteriolytic enzyme derived from streptomyces globisporus]. | | 1979 | 297060 |
| elastases from human and canine granulocytes, ii. interaction with protease inhibitors of animal, plant, and microbial origin. | inhibitors of animal, plant, and microbial origin were tested against human and canine granulocytic elastases. the trypsin-chymotrypsin inhibitors from dog submandibular glands, from soybeans (bowman-birk) and from chickpeas show strong interaction with these proteases (ki = 10(-8) - 10(-9)m). the trypsin-kallikrein inactivator of bovine organs (trasylol) is not active against granulocytic elastases or against human granulocytic cathepsin g. elastatinal, a specific inhibitor of elastases, isolat ... | 1977 | 300070 |
| hyperthermic effects of d-lysergic acid diethylamide (lsd) and its derivatives in rabbits and rats. | several d-lysergic acid diethylamide (lsd) derivatives including metabolites of lsd in animals liver and streptomyces such as d-lysergic acid ethyl, 2-hydroxyethylamide (leo), d-lysergic acid ethylamide (lae), d-norlysergic acid diethylamide (norlsd) and synthetic n6-allyl-d-norlysergic acid diethylamide (allyl-nor lsd) were studied with rabbits and rats. in rabbits, the order of the hyperthermic activities was allyl-norlsd greater than lsd greater than leo, lae, nor-lsd, and this order was para ... | 1977 | 303504 |
| characterization and regulation of anthranilate synthetase from a chloramphenicol-producing streptomycete. | in streptomyces sp. 3022a, anthranilate synthetase is composed of two non-identical subunits. the major subunit (molecular weight, 72,000) converts chorismic acid to anthranilic acid, using ammonia as the source of the amino group. the smaller subunit (molecular weight 28,000 to 29,000) confers on the enzyme the ability to use glutamine instead of ammonia as a substrate. in this study, reactivity with glutamine reached its maximum at ph 7.2 to 7.6, whereas that with ammonia increased linearly th ... | 1978 | 306386 |
| exogenous mycotic infections of the eye and adnexia. | three cases of exogenous mycotic infections of the eye are presented. the first is a case of keratitis in a patient suffering from glaucoma simplex who removed a foreign body from the cornea, caused by fusarium solani, generally known as a saprophytic soil and plant inhabitant. as factors predisposing the patient to infection, trauma to the cornea like injury by a foreign body, as well as the preexisting disease of the eye, are discussed. the other case illustrates the change in the pathogenicit ... | 1978 | 308789 |
| property of the beta-lactamase from streptomyces e750-3. | | 1978 | 309466 |
| production of beta-lactamase by non-streptomyces actinomycetales. | supernatants and whole cells from fermentation broths of micromonospora, nocardia, oerskovia, and other genera of the actinomycetales were examined for the presence of beta-lactamase activity by using the chromogenic cephalosporin 87/312. nearly 60% of the 250 isolates examined produced detectable levels of beta-lactamase. all enzyme preparations were active over a range of ph values from 6.5 to 8.2, with maximum activity occurring between 7.0 and 7.8. the preparations varied in their stability ... | 1979 | 311614 |
| physicochemical characterization of lymphocyte-activating factor (laf). | the laf produced by the mouse macrophage cell line, p388d1, is a single polypeptide chain of m.w. 12,000 to 16,000 daltons. native laf was destroyed by streptomyces griseus protease, but not by trypsin, chymotrypsin, and papain, although in the presence of 8 m urea, papain completely destroyed laf activity. laf did not bind to concanavalin a-sepharose, suggesting that laf does not contain significant amounts of mannosyl or glycosyl residues. since laf activity was not inactivated by a treatment ... | 1979 | 312860 |
| mitogenic effects of bacterial cell walls, their fragments, and related synthetic compounds on thymocytes and splenocytes of guinea pigs. | stimulation of [(3)h]thymidine incorporation of thymocytes and splenocytes from guinea pigs by various bacterial cell walls and their peptidoglycans, by enzymatic digests, and by synthetic muramyl dipeptides was studied as an indication of mitogenic activity. cell wall and peptidoglycan preparations, isolated from 19 strains belonging to 18 different species, definitely increased [(3)h]thymidine incorporation of thymocytes as well as splenocytes, regardless of mycolic acid contents as a non-pept ... | 1979 | 314932 |
| effects of experimental conditions on the interaction of filipin and pimaricin with cholesterol. | | 1979 | 316813 |
| four further antibiotics related to olivanic acid produced by streptomyces olivaceus: fermentation, isolation, characterisation and biosynthetic studies. | four beta-lactam antibiotics with beta-lactamase inhibitory activity mm 22380, mm 22381, mm 22382 and mm 22383 containing the carbapenem nucleus have been isolated from a culture of streptomyces olivaceus atcc 31365. fermentation conditions for their production and methods for their isolation are described. evidence for a biosynthetic link between these compounds and the previously described olivanic acid derivatives mm 4550, mm 13902 and mm 17880 is presented. | 1979 | 317283 |
| purification of beta-lactamase from streptomyces cellulosae by affinity chromatography on blue sepharose. | a beta-lactamase from culture supernatant of streptomyces cellulosae was purified about 1,450-fold to apparent homogeneity in polyacrylamide gel electrophoresis and isoelectric focusing on polyacrylamide gel sheet. the methods used were ammonium sulfate precipitation, cm-52 cellulose ion-exchange chromatography and affinity chromatography on blue sepharose cl-6b. the molecular weight was determined to be approximately 27,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. this valu ... | 1979 | 317285 |
| isolation and characterization of plasmids from streptomyces. | | 1979 | 317286 |
| a method of rapid wetting and synchronous germination of streptomycete spores. | | 1977 | 321309 |
| anthracyclines. | | 1977 | 321310 |
| production of polyene macrolide antibiotics. | | 1977 | 322452 |
| dna restriction site mapping of adenovirus type 16 with bami, ecori, hpai and sa/i. | | 1977 | 324798 |
| subunit structure of external invertase from saccharomyces cerevisiae. | because 50% of the mass of the external invertase of saccharomyces cerevisiae consists of carbohydrate, it has been extremely difficult to obtain an accurate molecular weight of this enzyme by centrifugal or electrophoretic techniques. however, on removing almost all of the oligosaccharide chains of this enzyme with the endo-beta-n-acetyl-glucosaminidase h from streptomyces plicatus, it has been possible to show that carbohydrate-free invertase is composed of two 60,000-dalton subunits. terminal ... | 1977 | 325007 |
| release of microorganisms from soil with respect to transmission electron microscopy viewing and plate counts. | | 1977 | 326180 |
| antibiotics produced by streptomyces olivaceus 142. ii. isolation, purification and activity spectrum of antibiotic wr 142-fpg. | a method of isolating antibiotic wr 142-fpg from the fermentation broth and mycelium of mutant fpg of the strain streptomyces olivaceus 142 has been developed. the method of purification of the active substance and activity spectrum agains microorganisms are described. some of the basic properties of two preparations are given: a) the preparation designed i, partly purified, containing a peptide moetye and b) the preparation designed ii, which is a homogeneous chromatographically preparation wit ... | 1977 | 326221 |