| a novel and remarkably thermostable ferredoxin from the hyperthermophilic archaebacterium pyrococcus furiosus. | the archaebacterium pyrococcus furiosus is a strict anaerobe that grows optimally at 100 degrees c by a fermentative-type metabolism in which h2 and co2 are the only detectable products. a ferredoxin, which functions as the electron donor to the hydrogenase of this organism was purified under anaerobic reducing conditions. it had a molecular weight of approximately 12,000 and contained 8 iron atoms and 8 cysteine residues/mol but lacked histidine or arginine residues. reduction and oxidation of ... | 1989 | 2542225 |
| effect of rice fiber on human fecal microflora. | the effects of brown rice, containing fourfold as much dietary fiber as polished rice, on the human fecal microflora were determined. significantly increased numbers of bifidobacterium adolescentis and enterococcus faecalis were observed during the brown rice intake, whereas the total counts and the numbers of bacteroides, eubacterium aerofaciens, and escherichia coli during the intake were lower than those before and after the intake. lower numbers of clostridia and low incidences of clostridiu ... | 1989 | 2547140 |
| purification and properties of ferredoxin and rubredoxin from butyribacterium methylotrophicum. | a ferredoxin and a rubredoxin from butyribacterium methylotrophicum, which displays a carbonyl-dependent acetyl-coenzyme a synthesis, were purified to electrophoretic homogeneity. the two electron carriers showed absorption spectra similar to those in clostridium species. the ferredoxin displayed absorption peaks at 280 and 391 nm, while rubredoxin displayed absorption peaks at 279, 382, and 482 nm. minimum molecular weights calculated from the respective amino acid compositions were 5,727 for f ... | 1989 | 2548997 |
| metabolism of leukotriene e4 to 5-hydroxy-6-mercapto7,9-trans-11,14-cis-eicosatetraenoic acid by microfloral cysteine-conjugate beta-lyase and rat cecum contents. | leukotriene e4 was incubated with cysteine-conjugate beta-lyase isolated from the intestinal bacterium eubacterium limosum. the reaction was terminated by addition of iodoacetic acid or dimethyl sulfate, and the products formed were isolated by reverse-phase high-performance liquid chromatography. the structures of two adducts of a metabolite were determined by uv spectroscopy, by gas-liquid radiochromatography, and by comparisons with chemically synthesized reference compounds. they were 5-hydr ... | 1989 | 2556967 |
| epidural abscess and subdural empyema. | epidural abscess and subdural empyema are serious intracranial infections that result in significant morbidity and mortality. frequently, they are secondary to sinusitis or middle ear disease, and the bacteria involved are inhabitants of the upper respiratory tract. symptoms may be mild and mimic the symptoms of the underlying infection. however, especially with subdural empyema, alteration in the level of consciousness and focal neurologic deficits are common. morbidity and mortality are minimi ... | 1989 | 2568981 |
| nucleotide sequence and expression of the glutamine synthetase structural gene, glna, of the archaebacterium methanococcus voltae. | the sequence of a 2,746-bp dna fragment of methanococcus voltae carrying the glna gene for glutamine synthetase (gs), was established. a 1,338-bp open reading frame (orf), encoding a 446-amino-acid polypeptide of 50,142 da, was defined as glna on the basis of its similarity to other glna genes and on the ability of a dna fragment carrying this orf to complement an escherichia coli gln- mutant. no sequence homology was found between sequences flanking the m. volae glna gene and other eubacterial ... | 1989 | 2575777 |
| cell wall fragments from major residents of the human intestinal flora induce chronic arthritis in rats. | to investigate the involvement of human intestinal flora in joint inflammation, cell wall fragments of 9 anaerobic gram positive bacteria of the human fecal flora were prepared and tested for arthropathic properties in the rat. a single intraperitoneal injection of cell wall fragments from eubacterium aerofaciens or bifidobacterium species induced persistent chronic arthritis, in contrast to those from eubacterium rectale, clostridium species and lactobacillus leichmanii. the results show that c ... | 1989 | 2585403 |
| primary structures of five ribosomal proteins from the archaebacterium halobacterium marismortui and their structural relationships to eubacterial and eukaryotic ribosomal proteins. | the complete amino acid sequences of ribosomal proteins l9, l20, l21/22, l24 and l32 from the archaebacterium halobacterium marismortui were determined. the comparison of the sequences of these proteins with those from other organisms revealed that proteins l21/22 and l24 are homologous to ribosomal protein yrp29 from yeast and l19 from rat, respectively, and that h. marismortui l20 is homologous to l30 from eubacteria. h. marismortui ribosomal protein l9 showed sequence homology to both l29 fro ... | 1989 | 2591382 |
| conserved sequence elements upstream and downstream from the transcription initiation site of the caulobacter crescentus rrna gene cluster. | the nucleotide sequence and in vivo transcription start sites for rrna, one of the two rrna gene clusters of the eubacterium caulobacter crescentus, have been determined. two transcription start sites, a major and minor, for the rrna gene cluster are located more than 700 nucleotides upstream from the 16 s rrna gene. transcription was detected from only the major start site in swarmer cells. but after the swarmer-to-stalked cell transition, transcription was detected from both rrna start sites a ... | 1989 | 2600967 |
| identification of phenolyl cobamide from the homoacetogenic bacterium sporomusa ovata. | phenolyl cobamide was isolated from cyanide extractions of the anaerobic eubacterium sporomusa ovata. the proposed corrinoid structure [co alpha,co beta-(monocyano,monoaquo)-phenolyl cobamide] has been deduced from 1h nmr, fast-atom-bombardment mass spectroscopy and ultraviolet/visible spectroscopy data. the complete corrinoid resembled p-cresolyl cobamide [co alpha,co beta-(monocyano,monoaquo)-p-cresolyl cobamide], which recently has been obtained from cyanide extractions of the same bacterium. ... | 1989 | 2606109 |
| ferredoxin and rubredoxin from butyribacterium methylotrophicum: complete primary structures and construction of phylogenetic trees. | complete amino acid sequences of ferredoxin and rubredoxin from butyribacterium methylotrophicum, a methylotrophic hetero-acetogen, were determined by combination of protease digestion, edman degradation, carboxypeptidase digestion, and/or partial acid hydrolysis. the ferredoxin was composed of 55 amino acids with a molecular weight of 5,732 excluding iron and sulfur atoms and showed a typical 2[4fe-4s]-type ferredoxin sequence with an internal repeat at the 14-23 and 42-51 positions. the rubred ... | 1989 | 2606914 |
| antimicrobial susceptibilities of eubacterium, peptostreptococcus, and bacteroides isolated from root canals of teeth with periapical pathosis. | eubacterium, peptostreptococcus, and bacteroides were isolated in high frequency from root canals with acute periapical inflammation. the antimicrobial susceptibilities of these strains were studied by determining minimum inhibitory concentrations of different agents. although all three kinds of isolates were susceptible to penicillins, the isolates other than black-pigmented bacteroides were less susceptible to cephems, tetracyclines, and macrorides with several resistant strains. all strains w ... | 1989 | 2607278 |
| characterization of anaerobic bacteria by using a commercially available rapid tube test for glutamic acid decarboxylase. | a rapid glutamic acid decarboxylase microdilution test for presumptive identification of certain anaerobic bacteria was marketed recently by carr-scarborough microbiologicals, inc., stone mountain, ga. the test was evaluated with 474 clinical isolates, representing 11 genera and 54 species, and was found to be a useful aid in the presumptive identification of bacteroides fragilis, b. distasonis, b. vulgatus, b. thetaiotaomicron, b. ovatus, b. uniformis, b. eggerthii, clostridium perfringens, c. ... | 1989 | 2644301 |
| evidence for several higher order structural elements in ribosomal rna. | comparative analysis of small subunit ribosomal rna sequences suggests the existence of two new higher order interactions: (i) a double-helical structure involving positions 505-507 and 524-526 (escherichia coli numbering) and (ii) an interaction between the region of position 130 and the helix located approximately between positions 180 and 195. in the first of these, one of the strands of the helix exists in the bulge loop, and the other strand exists in the terminal loop of a previously recog ... | 1989 | 2654936 |
| [the influence of cefpodoxime proxetil on the intestinal bacterial flora]. | the influence of cefpodoxime proxetil (cpdx-pr, cs-807), a new oral cephalosporin, on the intestinal bacterial flora was studied in tetra-contaminated mice and in pediatric patients. cpdx-pr dry syrup was administered at a dose of 10 mg/kg once a day for 5 consecutive days to mice contaminated with 4 different species of bacteria: escherichia coli, enterococcus faecalis, bacteroides fragilis and bifidobacterium breve. no notable changes were observed in fecal viable cell counts except that sligh ... | 1989 | 2681865 |
| development of a differential medium for bile salt hydrolase-active lactobacillus spp. | an agar plate assay was developed to detect bile salt hydrolase activity in lactobacilli. on lactobacillus-selective mrs or rogosa sl medium supplemented with taurodeoxycholic, taurocholic, or taurochenodeoxycholic acids, bile salt hydrolysis was manifested at two intensities: (i) the formation of precipitate halos around colonies or (ii) the formation of opaque granular white colonies. sixty-six lactobacilli were tested for bile salt hydrolase activity by both the plate assay and a sensitive ra ... | 1989 | 2705765 |
| nucleotide sequence of the gene coding for the elongation factor tu from the extremely thermophilic eubacterium thermotoga maritima. | | 1989 | 2714630 |
| thermus thermophilus 16s rrna is transcribed from an isolated transcription unit. | a cloned 16s rrna gene from the extreme thermophilic eubacterium thermus thermophilus hb8 was used to characterize the in vivo expression of the 16s rrna genes in this organism by nuclease s1 mapping. the gene represents an isolated transcription unit encoding solely 16s rrna. under exponential growth conditions, transcription was initiated at a single promoter, which represents the structural equivalent of escherichia coli rrn p2 promoters. the promoter-leader region was very similar to the e. ... | 1989 | 2722737 |
| isolation and characterization of xylose- and xylan-utilizing anaerobic bacteria. | by enrichment with xylose, nine mesophilic strains of anaerobic bacteria were obtained from various sources. two isolates appear to belong to the genus eubacterium. six other strains belong to the genus clostridium. three of the isolated strains utilized larch wood xylan. the percentage of utilization of xylose and xylan and the yield of fermentation end products--viz. acetic acid and butyric acid--are equivalent to that of clostridium acetobutylicum (atcc 824) and reported thermophilic strains. | 1989 | 2742371 |
| chemical composition of eubacterium alactolyticum cell wall peptidoglycan. | the mechanism of lysis of eubacterium alactolyticum cell walls by streptomyces albus g enzyme was studied. the analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of s. albus g enzyme was mainly due to d-alanyl-a2pm endopeptidase, n-acetylmuramyl-l-alanine amidase, n-acetylmuramidase and n-acetylglucosaminidase. e. alactolyticum cell wall peptidoglycan is composed mainly of g ... | 1989 | 2742450 |
| chemical composition of eubacterium nodatum cell wall peptidoglycan. | the structure of eubacterium nodatum cell wall peptidoglycan was investigated. the peptide subunit of e. nodatum peptidoglycan has the following structure: l-ala-d-glu (gly)-l-orn-d-ala. the carboxyl group of alanine occupying position 4 is attached to the delta-amino group of ornithine of an other subunit by the cross-linking bridge l-ala-l-ala-l-orn. all glycine molecules are connected with the alpha-carboxyl group of glutamic acid with the ratio being 0.5-1. the hydrolysis of e. nodatum pepti ... | 1989 | 2742451 |
| the effect of subminimal inhibitory concentrations of antimicrobial agents on three bacterial mixtures. | a test tube technique was developed to screen bacterial mixtures to detect interbacterial interactions that play a role in determining sensitivity to antimicrobial agents. we found 3 mixtures where these bacterial interactions change the sensitivity to antimicrobials or change the proportions of each bacterial species in the mixture. the mixtures were: fusobacterium nucleatum 102.3 and bacteroides endodontalis atcc 35406; f. nucleatum 102.3 and b. endodontalis bn11 a-f; and capnocytophaga ochrac ... | 1989 | 2762019 |
| megabase-sized linear dna in the bacterium borrelia burgdorferi, the lyme disease agent. | using pulsed-field gel electrophoresis we examined the genome of borrelia burgdorferi, a eubacterium of the spirochete phylum and the agent of lyme disease. a population of this species' cells was lysed in situ in agarose blocks. an abundant dna form that behaved as a linear duplex molecule under different electrophoretic conditions was found. the estimated size of the molecule was 950 kilobases. dna from two other genera of spirochetes did not enter the gel under these conditions. these studies ... | 1989 | 2762306 |
| impaired bacterial flora in human excluded colon. | we compared the rectal microflora of 16 patients with surgically excluded colorectum with 16 healthy controls. the cause of diversion was inflammatory bowel disease (n = 10), colon cancer (n = 3), miscellaneous (n = 3). six patients had a diversion colitis. in the excluded colorectum, the total bacterial count was only slightly lower than controls but the variety of the flora was significantly reduced. this reduction was confined to strict anaerobes, mainly the genus eubacterium and bifidobacter ... | 1989 | 2767506 |
| evidence for unlinked rrn operons in the planctomycete pirellula marina. | southern hybridization of rrnas to chromosomal bamhi-digested dna of the eubacterium pirellula marina revealed the presence of two sets of 16s and 23s rrna genes. the two copies of the 23s rrna genes, located on 11- and about 13-kilobase (kb) inserts, were isolated from a lambda bacteriophage charon 35 library. the 11-kb fragment was cloned directly into pbr322, while a 5.4-kb bamhi-psti rdna subfragment of the approximately 13-kb insert was cloned into puc18. both recombinant plasmids, ppi1100 ... | 1989 | 2768196 |
| improved assay for quantitating adherence of ruminal bacteria to cellulose. | a quantitative technique suitable for the determination of adherence of ruminal bacteria to cellulose was developed. this technique employs adherence of cells to cellulose disks and alleviates the problem of nonspecific cell entrapment within cellulose particles. by using this technique, it was demonstrated that the adherence of ruminococcus flavefaciens fd1 to cellulose was inhibited by formaldehyde, methylcellulose, and carboxymethyl cellulose. adherence was unaffected by acid hydrolysates of ... | 1989 | 2782879 |
| beta-lactam biosynthesis in a gram-negative eubacterium: purification and characterization of isopenicillin n synthase from flavobacterium sp. strain sc 12.154. | the occurrence, localization, and extraction of isopenicillin n-synthase (ipns) were investigated in the gram-negative low-level beta-lactam producer flavobacterium sp. strain sc 12.154, which forms deacetoxycephalosporin and excretes the cephabacin 7-formamidocephalosporin. ipns was detected with anti-ipns antibodies raised against the cephalosporium acremonium enzyme. the flavobacterium enzyme, whose molecular mass (38 kilodaltons) and cofactor requirements resemble those of the fungal and str ... | 1989 | 2793834 |
| mechanism of intestinal formation of deoxycholic acid from cholic acid in humans: evidence for a 3-oxo-delta 4-steroid intermediate. | 12 alpha-hydroxy-3-oxo-4-cholenoic acid coupled to an adenosine nucleotide has been shown to be a metabolite of cholic acid in the intestinal anaerobic bacteria, eubacterium species vpi 12708 (1987. j. biol. chem. 262: 4701-4707) and it has been suggested that this may be an intermediate in the conversion of cholic acid into deoxycholic acid. the possibility that the intestinal conversion of cholic acid into deoxycholic acid involves a 3-oxo-delta 4-steroid as an intermediate has been studied in ... | 1989 | 2794786 |
| erythromycin-induced digoxin toxicity. | the potential interaction between certain antibiotics and digoxin has been discussed in the literature; however, few cases of actual erythromycin-induced digoxin toxicity have been reported. we present a case in which an 86-year-old woman who was taking digoxin 0.25 mg/d developed probably digoxin toxicity after the administration of erythromycin for the treatment of otitis media and streptococcal pharyngitis. her digoxin concentration increased from a trough of 1.9 to 5.1 nmol/l six days after ... | 1989 | 2800579 |
| survey of metal tolerance in moderately halophilic eubacteria. | the tolerance patterns, expressed as mics, for 250 moderately halophilic eubacteria to 10 heavy metals were surveyed by using an agar dilution method. the moderate halophiles tested included 12 culture collection strains and fresh isolates representative of deleya halophila (37 strains), acinetobacter sp. (24 strains), flavobacterium sp. (28 strains), and 149 moderately halophilic gram-positive cocci included in the genera marinococcus, sporosarcina, micrococcus, and staphylococcus. on the basis ... | 1989 | 2802612 |
| isolation of eubacterium suis from sows with cystitis. | eubacterium suis was isolated from the bladders of 3 of 64 sows (4.7%) examined at slaughter. two of the sows had cystitis similar to other descriptions of e suis infections in sows. there was no histologic evidence of cystitis in the third sow from which e suis was recovered. in all 3 instances, a mixed infection was present. other bacteria present were predominantly gram-positive organisms. eubacterium suis cystitis may be more prevalent than previously suspected. the special culture condition ... | 1989 | 2808100 |
| enzymic activities of carbohydrate, purine, and pyrimidine metabolism in the anaeroplasmataceae (class mollicutes). | cell-free extracts of two strictly anaerobic mollicutes, anaeroplasma intermedium 5la and asteroleplasma anaerobium 161t, were tested for enzymic activities of intracellular carbohydrate metabolism. asteroleplasma anaerobium was also tested for enzymes of purine and pyrimidine metabolism. both organisms had enzymic activities associated with the nonoxidative portion of the pentose phosphate pathway, and with the embden-meyerhoff-parnas pathway. the 6-phosphofructokinase (pfk) of asteroleplasma a ... | 1989 | 2818126 |
| effect of high-fiber and high-oil diets on the fecal flora of swine. | six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. after 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. fifty colonies were randomly selected from each fecal sample, and isolates were characterized to identify a representative cross section of the fecal flora. the bacterial composition of the fecal flora differed between basal and high-f ... | 1987 | 2821900 |
| effect of dietary copper sulfate, aureo sp250, or clinoptilolite on ureolytic bacteria found in the pig large intestine. | the predominant ureolytic bacteria in the pig large intestine were determined while growing pigs were fed a basal diet or basal diet plus copper sulfate, aureo sp250, or clinoptilolite. fecal samples were collected from four pigs fed each diet at 3, 9, and 14 weeks and analyzed for total colony counts and percent ureolytic bacteria. fecal urease activity, ammonia nitrogen, and identity of the ureolytic bacteria were determined at 14 weeks. copper sulfate and aureo sp250 reduced the number of ure ... | 1987 | 2823707 |
| nucleotide sequence and regulation of a gene involved in bile acid 7-dehydroxylation by eubacterium sp. strain vpi 12708. | eubacterium sp. strain vpi 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. at least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. one of these, of molecular weight 27,000 (p-27), was shown to be involved in the 7-dehydroxylation reaction sequence. the gene coding for p-27 was cloned, and the entire dna sequence for the protein-coding region was determined. in addition, sequence information was ... | 1988 | 2834320 |
| in vitro activities of daptomycin (ly146032) and paldimycin (u-70,138f) against anaerobic gram-positive bacteria. | the in vitro activities of daptomycin (ly146032), paldimycin (u-70,138f), vancomycin, and penicillin g against 344 clinical isolates of anaerobic gram-positive bacteria were determined by an agar dilution method in calcium-supplemented (50 micrograms/ml) wilkins-chalgren medium, using an inoculum of 10(5) cfu. daptomycin demonstrated excellent activity against a broad range of anaerobic gram-positive cocci and bacilli, including peptostreptococcus, eubacterium, bifidobacterium, actinomyces, prop ... | 1988 | 2840019 |
| asni: a novel class ii restriction endonuclease from arthrobacter sp., strain n-cm, recognizing 5'-at/taat-3'. | a new class ii restriction endonuclease, asni, with a novel sequence specificity was isolated from the gram-positive eubacterium arthrobacter species, strain n-cm. asni recognizes the unambiguously defined palindromic hexanucleotide (formula: see text) consisting of a- and t-residues. the novel enzyme in the presence of mg2+ cleaves specifically both strands as indicated by the arrows. the staggered cuts generate 5'-protruding ends with single-stranded 5'-ta-3' dinucleotide extensions. the novel ... | 1988 | 2841156 |
| fatal frontal sinusitis due to neisseria sicca and eubacterium lentum. | infectious sinusitis may on occasion be associated with meningitis, subdural empyema, epidural empyema, brain abscess, or osteomyelitis. we report a 29-year-old male patient with frontal sinusitis who developed all of these intracranial complications due to two previously unreported causative organisms, neisseria sicca and eubacterium lentum. the fulminant and fatal course resulting from locally invasive disease underscores the importance of early diagnosis and proper treatment of these complica ... | 1986 | 2873255 |
| haemagglutinating and hydrophobic properties of corynebacterium (eubacterium) suis. | | 1986 | 2886018 |
| the plasma membrane atpase of the thermoacidophilic archaebacterium sulfolobus acidocaldarius. purification and immunological relationships to f1-atpases. | the plasma-membrane-associated atpase of the thermoacidophilic archaebacterium sulfolobus acidocaldarius characterized in a previous work [m. lübben & g. schäfer (1987) eur. j. biochem. 164, 533-540] has been solubilized. it can be easily removed from the membrane by mild treatment with zwitterionic detergents, therefore it appears to be a peripheral membrane protein analogous to the soluble f1-atpase of eubacteria and eukaryotes. further purification has been achieved by subsequent gel permeati ... | 1987 | 2887427 |
| ischaemic mouse thigh model for evaluation of pathogenicity of non-clostridial anaerobes. | a mouse thigh model has been devised in which the growing culture of non-clostridial anaerobe in the ischaemic tissue produces inflammatory swelling and death. the swelling of the right thigh served as an index of pathogenicity of the test strain in comparison to the negatively reacting left thigh which received injection of the control strain of bifidobacterium infantis. actinomyces naeslundii exceptionally caused death in all animals within 24 h. mortality and thigh swelling were pronounced (g ... | 1989 | 2914729 |
| mutation of bacillus firmus of4 to duramycin resistance results in substantial replacement of membrane lipid phosphatidylethanolamine by its plasmalogen form. | mutant strains of alkalophilic bacillus firmus of4 that were selected for resistance to duramycin had greatly reduced levels of membrane diacylphosphatidylethanolamine, as had been found in studies of such mutants of bacillus subtilis. in the b. firmus strains, however, substantial levels of plasmenylethanolamine were found. this is an unusual membrane component for an aerobic eubacterium, but the presence of trace amounts even in the wild type was confirmed in experiments with 32pi-labeled grow ... | 1989 | 2921248 |
| purification and properties of phloroglucinol reductase from eubacterium oxidoreducens g-41. | phloroglucinol reductase was purified 90-fold to homogeneity from the anaerobic rumen organism eubacterium oxidoreducens strain g-41. the enzyme is stable in the presence of air and is found in the soluble fraction after ultracentrifugation of cell extract. ion-exchange, hydrophobic interaction, and affinity chromatography were used to purify the enzyme. the native mr is 78,000, and the subunit mr is 33,000 indicating an alpha 2 homodimer. the enzyme is specific for phloroglucinol and nadph. the ... | 1989 | 2925649 |
| oral flora of mongrel and beagle dogs with periodontal disease. | the plaque flora was studied in adult mongrel and beagle dogs with periodontal disease. gingival plaque from maxillary premolars was removed and cultured on various growth media. the flora in all dogs was composed of mostly anaerobic gram negative rods. bacteroides asaccharolyticus was found in the highest proportion of plaque samples from mongrel dogs, and decomposed hydrogen peroxide suggesting catalase activity. fusobacterium nucleatum was found in higher proportion in the plaque of beagle do ... | 1989 | 2927018 |
| studies on mixed populations of human intestinal bacteria grown in single-stage and multistage continuous culture systems. | mixed intestinal bacteria were grown for 336 h in two identical single-stage chemostats at low growth rates in a carbohydrate-limited medium. complex bacterial populations were maintained and anaerobes always outnumbered aerobes. the predominant organisms belonged to the genera bacteroides, bifidobacterium, lactobacillus, clostridium, eubacterium, propionbacterium, peptococcus, and peptostreptococcus. bacteroides species predominated in both fermentors, particularly b. ovatus and b. thetaiotaomi ... | 1989 | 2930171 |
| establishment of specific pathogen-free rabbits by inoculation of hysterectomy-derived rabbits with fecal suspension and anaerobic bacteria. | establishment of a specific pathogen-free (spf) rabbit colony was attempted by transferring a total of 127 hysterectomy-derived rabbits directly into a barrier room where spf rats had been reared. all rabbits except four died of mucoid enteritis-like diarrhea during 2 and 6 weeks of age. a second experiment consisted of four groups of hysterectomy-derived rabbits including: (1) noninoculated controls; (2) rabbits inoculated with suspension of feces from the survivors; (3) rabbits inoculated with ... | 1987 | 2963932 |
| biosynthesis of vitamin b-12 in anaerobic bacteria. experiments with eubacterium limosum and d-erythrose 14c-labeled in different positions. | in anaerobic microorganisms the origin of c atoms 2 and 4-7 of the 5,6-dimethylbenzimidazole moiety of vitamin b-12 is still unknown. in order to tackle this problem we added several 14c-labeled putative precursors to eubacterium limosum fermentations. the degradation of the isolated vitamin b-12 revealed that only d-erythrose, 14c-labeled in different positions, was efficiently incorporated into the 5,6-dimethylbenzimidazole part. the 5,6-dimethylbenzimidazole obtained from an experiment with d ... | 1988 | 2968906 |
| generation of a large, protonophore-sensitive proton motive force and ph difference in the acidophilic bacteria thermoplasma acidophilum and bacillus acidocaldarius. | the mechanism by which acidophilic bacteria generate and maintain their cytoplasmic ph close to neutrality was investigated. for this purpose we determined the components of proton motive force in the eubacterium bacillus acidocaldarius and the archaebacterium thermoplasma acidophilum. after correction for probe binding, the proton motive force of untreated cells was 190 to 240 mv between external ph 2 and 4. anoxia diminished total proton motive force and the transmembrane ph difference by 60 t ... | 1985 | 2981803 |
| two new restriction endonucleases draii and draiii from deinococcus radiophilus. | in addition to recently characterized drai (1), two new type ii restriction endonucleases, draii and draiii, with novel site-specificities were isolated and purified from deinococcus radiophilus atcc 27603. draii and draiii recognize the hepta- and nonanucleotide sequences (sequence in text) the cleavage sites within both strands are indicated by arrows. the recognition sequences were established by mapping of the cleavage sites on pbr322 (draii) and fd109 rf dna (draiii). the sequence specifiti ... | 1985 | 2987827 |
| characterization of membrane lipids of a general fatty acid auxotrophic bacterium by electron spin resonance spectroscopy and differential scanning calorimetry. | lipids in the plasma membrane of the general fatty acid auxotroph butyrivibrio s2 pack as a bilayer that is characterized by a high order and high motional anisotropy and a low membrane fluidity compared to mammalian plasma membranes. lipid packing as determined by the electron spin resonance (esr) order parameter and membrane fluidity as measured by esr correlation times are, however, comparable to those of other bacterial membranes. membranes of the organism grown with saturated fatty acids of ... | 1985 | 3000428 |
| does cytomegalovirus play a role in community-acquired pneumonia? | cytomegalovirus (cmv) is recognized as an important pathogen in the immuno-suppressed patient. sporadic case reports of cytomegalovirus community-acquired pneumonia have appeared. we studied 443 patients with community-acquired pneumonia requiring hospitalization to define the role of cytomegalovirus in this illness. four patients (0.9%) had good evidence that cytomegalovirus caused their pneumonia: 2 had the virus isolated from pulmonary tissue and 2 had cytomegalovirus inclusion bodies visuali ... | 1985 | 3000667 |
| [in vitro activity of an ofloxacin-metronidazole combination against anaerobic bacteria. kinetics of the action of metronidazole against bacteroides fragilis]. | the activity of metronidazole combined with ofloxacin was investigated by the checkerboard method in liquid medium against 60 obligate anaerobes. the bacteriostatic effect of the combination was assessed by calculating the fic index. two metronidazole resistant strains of propionibacterium acnes (mic greater than 32 mg/l) were inhibited by 0.125 mg/l of this former antibiotic in presence of an ofloxacin concentration equal to half the value of the mic. on the 58 other anaerobic strains, the comb ... | 1988 | 3043345 |
| the autotrophic pathway of acetate synthesis in acetogenic bacteria. | | 1986 | 3096193 |
| important properties in the differentiation of gram positive non-sporing rods in the genera propionibacterium, eubacterium, actinomyces and bifidobacterium. | | 1986 | 3103235 |
| another polysaccharide antigen of eubacterium saburreum with heptose as the main constituent. | the polysaccharide antigen of eubacterium saburreum strains l76, reacting by precipitation and complement-fixation, has d-glycero-d-galacto-heptose as the main component, and, in addition, o-acyl groups. | 1987 | 3109209 |
| evolution of organisms and organelles as studied by comparative computer and biochemical analyses of ribosomal 5s rna structure. | the results documented in this publication demonstrate that for evolutionary studies the ribosomal 5s rrna is a suitable object for such an investigation and that as many methods as possible should be consulted. in this study the results of biochemical and chemical experiments were combined with those of computer sequence analyses, and they revealed that these methods complement each other nicely. we are currently at a state at which we are able to well define the secondary structures of the 5s ... | 1987 | 3113313 |
| three-dimensional crystals of ribosomes and their subunits from eu- and archaebacteria. | ordered three-dimensional crystals of 70s ribosomes as well as of 30s and 50s ribosomal subunits from various bacteria (e. coli, bacillus stearothermophilus, thermus thermophilus and halobacterium marismortui) have been grown by vapour diffusion in hanging drops using mono- and polyalcohols. a new compact crystal form of 50s subunits has been obtained, and it is suitable for crystallographic studies at medium resolution. in addition, from one crystal form large crystals could be grown in x-ray c ... | 1987 | 3124853 |
| the primary structure of the ribosomal a-protein (l12) from the halophilic eubacterium haloanaerobium praevalens. | the ribosomal a-protein, equivalent to the ribosomal protein l12 from escherichia coli, has been sequenced from the anaerobic halophilic eubacterium haloanaerobium praevalens (dsm 2228). the protein contains 122 amino acids, has a composition of asp6, asn2, thr2, ser6, glu22, pro2, gly13, ala19, val12, met4, ile5, leu11, phe3, lys14, arg1 and has a molecular weight of 12,691. the hydrophilicity profile was determined for this protein. a phylogenetic or cluster tree was calculated from computer a ... | 1987 | 3126821 |
| antitumor effect of normal intestinal microflora on ehrlich ascites tumor. | in order to investigate the antitumor activity of intestinal microflora, the constitution of normal flora was examined in humans, guinea pigs and mice. it was clarified that eubacterium, bifidobacterium and bacteroides were the predominant bacterial genera in humans. in addition, neither clostridium nor enterobacteriaceae was detected in guinea pigs and neither clostridium nor bifidobacterium was present in mice. total bacterial counts in tumor-bearing mice were reduced in comparison with those ... | 1988 | 3128498 |
| antitumor activity and its properties of eubacterium lentum. | in the present study, some basic effects of eubacterium lentum (tyh-11), isolated from normal intestinal flora, upon the immune system and various experimental tumor cell lines were investigated. e. lentum showed no direct cytotoxicity against ehrlich ascites tumor, while serratia marcescens (ty-142) did show direct cytotoxicity. e. lentum presented striking antitumor activity which differed according to the injection route and time. this strain showed antitumor activity against 11 experimental ... | 1988 | 3128499 |
| bacterial surface proteins. some structural, functional and evolutionary aspects. | the structure of several eubacterial and archaebacterial surface (glyco)proteins as determined by three-dimensional electron microscopy is described. particular emphasis is placed on surface proteins which interact with membranes. some structure-function relationships deduced from the structural information, such as shape maintenance and molecular recognition phenomena, are discussed. | 1988 | 3129041 |
| ca2+-stabilized oligomeric protein complexes are major components of the cell envelope of "thermus thermophilus" hb8. | the major cell envelope proteins of the gram-negative thermophilic eubacterium "thermus thermophilus" gave an electrophoretical pattern characterized by two well-defined groups of bands. one of them showed up as four regularly spaced proteins (hmrps) with mrs higher than 310,000, a value corresponding to the smaller hmrp. the second one was formed by two proteins with mrs of 100,000 (p100) and 84,000 (p84). hmrps p100 and p84 were apparently located in the outer layer of the cell envelope, as in ... | 1988 | 3131301 |
| characteristic views of prokaryotic 50s ribosomal subunits. | multivariate statistical analysis and classification techniques are powerful tools in sorting noisy electron micrographs of single particles according to their principal features, enabling one to form average images with an enhanced signal-to-noise ratio and a better reproducible resolution. we apply this methodology here to determining the characteristic views of the large (50s) ribosomal subunits from the eubacterium escherichia coli and the archaebacteria methanococcus vannielii, sulfolobus s ... | 1987 | 3131536 |
| a unique type of eubacterial 5s rrna in members of the order planctomycetales. | analysis of the 5s ribosomal rna from members of the eubacterial order planctomycetales, i.e., planctomyces, pirella, gemmata, and isosphaera, reveals several unexpected features. firstly, the primary structures are significantly shorter than those of the majority of eubacteria and vary in length between 109 and 111 nucleotides. secondly, the lack of an insertion at position 66 is a feature not encountered before in prokaryotic 5s rrnas. thirdly, as compared to the proposed eubacterial "minimal" ... | 1988 | 3137349 |
| analysis and characterization of the folates in the nonmethanogenic archaebacteria. | a detailed analysis of the folate coenzymes in the nonmethanogenic archaebacteria has been performed. by using the lactobacillus casei microbiological assay for folates, the levels of folates in sulfolobus solfataricus and sulfolobus acidocaldarius were found to be 3.7 and 8.3 ng/g (dry weight) of cells, respectively, compared with 88,000 and 28,000 ng/g (dry weight) of cells in halobacterium halobium and halobacterium strain gn-1, respectively. the levels of folates found in the sulfolobus spp. ... | 1988 | 3139633 |
| evidence for a multigene family involved in bile acid 7-dehydroxylation in eubacterium sp. strain vpi 12708. | eubacterium sp. strain vpi 12708 is a human intestinal isolate which has an inducible bile acid 7-dehydroxylation activity. at least two cholic acid-induced polypeptides, with molecular masses of 27,000 and 45,000 daltons, respectively, coelute with bile acid 7-dehydroxylation activity. the 45,000-dalton polypeptide appears to be encoded by a cholic acid-induced mrna species of greater than 6 kilobases, which suggests that the gene coding for this polypeptide is part of a larger operon. a gene h ... | 1988 | 3170477 |
| glycyrrhizin stimulates growth of eubacterium sp. strain glh, a human intestinal anaerobe. | eubacterium sp. strain glh was isolated from human feces and produced two kinds of beta-d-glucuronidase (ec 3.2.1.31), one new enzyme specific for glycyrrhizin (gl) and the other for phenyl beta-d-glucuronides. gl or p-nitrophenyl-mono-beta-d-glucuronide (pnpg) stimulated the production of gl or pnpg beta-glucuronidases and the growth of strain glh in a basal medium lacking carbohydrate. d-glucuronic acid also stimulated the growth of the bacterium, but glycyrrhetic acid did not. the increase of ... | 1988 | 3178209 |
| isolation and identification of intestinal steroid-desulfating bacteria from rats and humans. | we isolated 12 strictly anaerobic steroid-3-sulfate-desulfating strains from the intestinal floras of rats and humans. two strains (s1 and s2) of the same atypical clostridium species and an atypical lactobacillus strain (termed r9) were obtained from rats. the human isolates were identified as eubacterium cylindroides (two strains, h1 and h2), peptococcus niger (two strains, h4 and h89), and clostridium clostridiiforme. we also isolated, from different human fecal samples, four strains of pheno ... | 1988 | 3178214 |
| tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca. | tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe. | 1988 | 3190205 |
| amino acid sequences of ribosomal proteins s11 from bacillus stearothermophilus and s19 from halobacterium marismortui. comparison of the ribosomal protein s11 family. | the complete amino acid sequences of ribosomal proteins s11 from the gram-positive eubacterium bacillus stearothermophilus and of s19 from the archaebacterium halobacterium marismortui have been determined. a search for homologous sequences of these proteins revealed that they belong to the ribosomal protein s11 family. homologous proteins have previously been sequenced from escherichia coli as well as from chloroplast, yeast and mammalian ribosomes. a pairwise comparison of the amino acid seque ... | 1988 | 3191988 |
| conversion of iq, a dietary pyrolysis carcinogen to a direct-acting mutagen by normal intestinal bacteria of humans. | the dietary carcinogen, 2-amino-3-methyl-3h-imidazo[4,5-f]quinoline (iq) is mutagenic in the salmonella/microsomal mutagenicity assay when activated by microsomal enzymes. iq is found in many cooked foods, notably fried beef and pork. in laboratory rodents iq is carcinogenic. we showed that mixed and pure cultures of human intestinal anaerobes, notably eubacterium spp., metabolized iq to 2-amino-3,6-dihydro-3-methyl-7h-imidazo[4,5-f]quinoline-7-one (hoiq). unlike iq, both the synthetic and bacte ... | 1988 | 3200256 |
| outer membrane protein pattern of eubacterium plautii. | the outer membrane sds-page pattern of eubacterium plautii was characterized by a large number of surface exposed low- and high-molecular-mass proteins. silver stainable carbohydrate was not present. the pattern was clearly distinct from those of outer membrane preparations of eubacterium saburreum and fusobacterium nucleatum. the results are compatible with a gram-positive cell wall structure in e. plautii. | 1988 | 3221197 |
| central nervous system infection associated with anaerobic bacteria in two dogs and two cats. | central nervous system (cns) infection caused by anaerobic bacteria (including bacteroides, fusobacterium, peptostreptococcus, and eubacterium) was diagnosed in two dogs and two cats. in one dog there was extensive meningomyeloencephalitis, presumably the result of hematogenous spread of bacteria from lung abscesses and bacterial endocarditis. subdural empyema of unknown origin was found in a second dog and two cats. clinical signs in all four animals included mental depression and focal neurolo ... | 1988 | 3230556 |
| heat shock proteins in the moderately halophilic bacterium deleya halophila: protective effect of high salt concentration against thermal shock. | the moderately halophilic eubacterium deleya halophila grown in medium containing 1 m or 2.5 m nacl was heat-shocked at various temperatures and the electrophoretic patterns of pulse-labelled proteins were examined. several polypeptides were induced (heat shock proteins, or hsp) at all temperatures. however, the level of induction of some hsp was dependent on the severity of the thermal shock as well as the salt concentration of the growth medium. time course studies revealed that synthesis of s ... | 1988 | 3252902 |
| ultrastructure of a novel anaerobic gram-positive nonsporing rod from dental root canal. | a novel anaerobic gram-positive rod, strain es4c, was isolated from a dental root canal infection. the isolate did not produce acids from carbohydrates and showed no glycosidase activity. most biochemical reactions were identical to clostridium malenominatum with the exception of the production of three aminopeptidases. in addition, no spores were detected. a tetragonally arranged surface layer was consistently found by electron microscopy. the ultrastructure of closely related eubacterium spp. ... | 1988 | 3277260 |
| cefotetan: a review of the microbiologic properties and antimicrobial spectrum. | cefotetan (formerly ici 156834 and ym09330) is a 7-methoxy cephalosporin possessing some advantageous antimicrobial spectrum, safety, and pharmacokinetic characteristics compared with other so-called second-generation cephalosporins. the published literature was reviewed and the cefotetan quantitative susceptibility testing data from nearly 31,000 isolates was tabulated. against 15,769 enteric bacilli, cefotetan was observed to have a potency and spectrum more closely resembling a third-generati ... | 1988 | 3287964 |
| bacterial metabolism of natural and synthetic sex hormones undergoing enterohepatic circulation. | steroids undergoing enterohepatic circulation are exposed to bacterial metabolism particularly by obligate anaerobes which account for 99.99% of the fecal flora. the most common transformation is hydrolysis of conjugated steroids. the glucuronidases are synthesized by escherichia coli and bacteroides species. the bacterial catabolism of unconjugated steroids may be considered under several headings: 1. reduction of ring-a due to clostridia species synthesizing specific enzymes; c. paraputrificum ... | 1987 | 3320550 |
| 5-aminolevulinic acid formation from glutamate via the c5 pathway in clostridium thermoaceticum. | a cell-free extract of the anaerobic eubacterium, clostridium thermoaceticum, catalyzes the synthesis of 5-aminolevulinic acid (ala) from glutamate via the c5 pathway. the enzyme reaction resembles that of higher plants and algae in cofactor requirements and sensitivity to ribonuclease. from the phylogenetic distribution it is proposed that the c5 pathway evolved earlier than the ala synthase pathway. | 1988 | 3342879 |
| biosynthesis of vitamin b-12 in anaerobic bacteria. experiments with eubacterium limosum on the origin of the amide groups of the corrin ring and of n-3 of the 5,6-dimethylbenzimidazole part. | the pathway of vitamin b-12 biosynthesis in anaerobic bacteria differs in several respects from the pathway found in aerobic or aerotolerant microorganisms. the aim of this investigation was to elucidate the formation of the 5,6-dimethylbenzimidazole part and the amide groups of vitamin b-12 in anaerobic bacteria. [15n]ammonium chloride or l-[amido-15n]glutamine or a mixture of [15n]ammonium sulfate and [15n]glycine was added to fermentations with eubacterium limosum. the vitamin b-12 isolated f ... | 1988 | 3345751 |
| characterization of the pyrogallol-phloroglucinol isomerase of eubacterium oxidoreducens. | cell extracts of eubacterium oxidoreducens, in the presence of dimethyl sulfoxide, catalyzed the conversion of pyrogallol to phloroglucinol with methyl sulfide as a product. the isomerization reaction also proceeded when 1,2,3,5-benzenetetrol was present rather than dimethyl sulfoxide. an assay to quantitate this activity was developed. the assay followed the disappearance of 1,2,4-benzenetriol as determined colorimetrically after incubation with sodium molybdate at neutral ph. the products of t ... | 1988 | 3372475 |
| metabolism of the 18o-methoxy substituent of 3-methoxybenzoic acid and other unlabeled methoxybenzoic acids by anaerobic bacteria. | o-methyl substituents of aromatic compounds can provide c1 growth substrates for facultative and strict anaerobic bacteria isolated from diverse environments. the mechanism of the bioconversion of methoxylated benzoic acids to the hydroxylated derivatives was investigated with a model substrate and cultures of one anaerobic consortium, eight strict anaerobic bacteria, and one facultative anaerobic microorganism. using high-pressure liquid chromatography and gas chromatography-mass spectral analy ... | 1988 | 3389815 |
| the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria. | the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ... | 1988 | 3396488 |
| induction of chronic arthritis in rats by cell wall fragments of anaerobic coccoid rods isolated from the faecal flora of patients with crohn's disease. | crohn's disease and ulcerative colitis are accompanied by seronegative arthritis in about one fifth of the cases. in the present study, cell wall fragments from major residents such as eubacterium, coprococcus and peptostreptococcus species, isolated from the faecal flora of patients with crohn's disease, were tested for properties to induce chronic arthritis in lewis rats. cell wall fragments from eubacterium contortum strains me44 and me47 were found to induce chronic arthritis; peptostreptoco ... | 1988 | 3410168 |
| a comparison of the reactivity of eubacterium species with localized and serum immunoglobulins from rapidly progressive and adult periodontitis patients. | immunoglobulins in sera and in supernatant fluids of explant cultures of diseased gingival tissues from 20 rapidly progressive and 20 adult periodontitis sites were tested by an elisa assay for reactivity with typed strains of eubacterium alactolyticum, e. brachy, e. limosum and e. nodatum. immunoglobulins present in tissue culture fluids from both rapidly progressive and adult periodontitis samples reactive with e. brachy and e. nodatum were significantly greater (p less than 0.05) than those r ... | 1988 | 3422290 |
| effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes. | the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ... | 1987 | 3426214 |
| methanogenic degradation of acetone by an enrichment culture. | an anaerobic enrichment culture degraded 1 mol of acetone to 2 mol of methane and 1 mol of carbon dioxide. two microorganisms were involved in this process, a filament-forming rod similar to methanothrix sp. and an unknown rod with round to slightly pointed ends. both organisms formed aggregates up to 300 micron in diameter. no fluorescing bacteria were observed indicating that hydrogen or formate-utilizing methanogens are not involved in this process. acetate was utilized in this culture by the ... | 1987 | 3442449 |
| purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine. | a novel type of arylsulfotransferase was purified from eubacterium a-44, one of the predominant bacteria of human intestine. the enzyme (mr 315 000) was composed of four identical subunits (mr 80 000) whose n-terminal amino acids were arginine. pi and optimal ph of the enzyme were 3.9 and 8-9, respectively. the apparent km for p-nitrophenylsulfate using tyramine as an acceptor substrate and that for tyramine using p-nitrophenylsulfate as a donor substrate were determined to be 0.104 mm and 3.5 m ... | 1986 | 3460636 |
| [fundamental and clinical studies of imipenem/cilastatin sodium in the field of obstetrics and gynecology]. | fundamental and clinical studies of a new carbapenem antibiotic, imipenem/cilastatin sodium (mk-0787/mk-0791), were carried out to evaluate the drug in treating infections of the female genital organs. the following results were obtained: in 4 patients undergoing hysterectomy, the penetration of mk-0787 and mk-0791 into female genital organ tissues was studied following a 30-minute intravenous drip infusion of mk-0787/mk-0791 500 mg/500 mg. plasma levels of mk-0787 and mk-0791 in cubital venous ... | 1986 | 3463786 |
| comparative serum bactericidal activity against test anaerobes in volunteers receiving imipenem, clindamycin, latamoxef and metronidazole. | ten healthy volunteers received on separate days the following regimens: imipenem 500 mg, clindamycin 600 mg, latamoxef 1 g, and metronidazole 500 mg. the antibiotics were given intravenously as an infusion over 15 min. blood samples were obtained before and 30 min, 1 and 6 h after the start of the infusion. serum bacteriostatic and bactericidal activities were measured against the following strains of strict anaerobes: two strains of bacteroides fragilis, one strain each of b. vulgatus, b. thet ... | 1987 | 3471750 |
| in vitro study of the antibacterial activity of ofloxacin against recent clinical isolates. | the in vitro activity of ofloxacin, a new broad-spectrum antimicrobial agent, was studied by a standardized single disc method. a total of 990 clinical isolates were tested, including 20 strains of anaerobic bacteria. ofloxacin was highly active against 683 strains (70.41%), had intermediate activity against 109 (11.23%) and had no activity against 178 (18.35%). ofloxacin was highly active against e. coli, klebsiella sp., citrobacter sp., proteus mirabilis, proteus morganii, salmonella sp., camp ... | 1987 | 3476290 |
| molecular cloning of bile acid 7-dehydroxylase from eubacterium sp. strain vpi 12708. | eubacterium sp. strain vpi 12708 is a human intestinal bacterium which contains an inducible bile acid 7-dehydroxylase. two-dimensional polyacrylamide gel electrophoresis showed that at least four new polypeptides were synthesized after exposure of growing cells to sodium cholate. one of these, of molecular weight 27,000 (pp-27), was implicated in 7-dehydroxylase catalysis. pp-27 was purified to greater than 95% homogeneity by deae-cellulose chromatography, high-pressure liquid chromatographic g ... | 1987 | 3549693 |
| ribosomes of the extremely thermophilic eubacterium thermotoga maritima are uniquely insensitive to the miscoding-inducing action of aminoglycoside antibiotics. | poly(u)- and poly(ug)-programmed cell-free systems were developed from the extreme thermophilic, anaerobic eubacterium thermotoga maritima, and their susceptibility to aminoglycoside and other antibiotics was assayed at a temperature (75 degrees c) close to the physiological optimum (80 degrees c) for cell growth and in vitro polypeptide synthesis, using a bacillus stearothermophilus system as the reference. the synthetic capacity of the thermotoga assay mixture was abolished by the eubacterium- ... | 1988 | 3410830 |
| cecal and fecal bacterial flora of the mongolian gerbil and the chinchilla. | the mongolian gerbil is being increasingly used as a laboratory animal and as a pet. both chinchillas and gerbils are used as animal models for otitis media and other otic research. previously, only incomplete information was available regarding the indigenous bacterial flora of the lower intestinal tracts of these coprophagic animals. using the strict anaerobic methodology of the virginia polytechnic institute anaerobe laboratory, we studied the predominant bacterial flora of the cecum and feca ... | 1988 | 3389814 |
| the three-dimensional structures of s-layers of two novel eubacterium species isolated from inflammatory human processes. | the three-dimensional structures of the crystalline surface layers of two species of eubacteria have been determined by electron microscopy and computerized image processing. the s-layer of eubacterium sp. es4c has tetragonal symmetry, with a unit cell spacing of 10.6 nm and a thickness of 9.5 nm, while that of eubacterium sp. ahn 990 has hexagonal symmetry a = b = 15.7 nm and a thickness of 13 nm. the resolutions in the reconstructions were 2.5 nm and 1.8 nm, respectively. the reconstruction of ... | 1988 | 3367785 |
| additional characteristics of one-carbon-compound utilization by eubacterium limosum and acetobacterium woodii. | growth characteristics of eubacterium limosum and acetobacterium woodii during one-carbon-compound utilization were investigated. e. limosum rf grew with formate as the sole energy source. formate also replaced a requirement for co2 during growth with methanol. growth with methanol required either rumen fluid, yeast extract, or acetate, but their effects were not additive. cultures were adapted to grow in concentrations of methanol of up to 494 mm. growth occurred with methanol in the presence o ... | 1987 | 3579266 |
| duodenal bile acids among children: keto derivatives and aerobic small bowel bacterial overgrowth. | duodenal bile acids, identified by gas-liquid chromatography (glc) and gas chromatography-mass spectrometry (gc-ms), were correlated with quantitative aerobic and anaerobic duodenal culture in 26 children with enteropathies. four patients whose duodenal fluid contained either greater than or equal to 10(6) gram-negative aerobes or greater than or equal to 10(6) aerobic lactobacilli per milliliter had a significantly greater molar percentage of keto-bile acids (32.3 +/- 8.4%) than did 19 controls ... | 1987 | 3320324 |
| cell wall and lipid composition of isosphaera pallida, a budding eubacterium from hot springs. | isosphaera pallida is an unusual gliding, budding eubacterium recently isolated from north american hot springs. electron micrographs of ultrathin sections revealed a cell wall atypical of eubacteria: two electrondense layers separated by an electron-transparent layer, with no evident peptidoglycan layer. growth was not inhibited by penicillin. cell walls were isolated from sheared cells by velocity sedimentation. the rigid-layer fraction, prepared from cell walls by treatment with boiling 10% s ... | 1987 | 3584067 |
| glycyrrhizin beta-d-glucuronidase of eubacterium sp. from human intestinal flora. | | 1987 | 3594680 |
| catabolic enzymes of the acetogen butyribacterium methylotrophicum grown on single-carbon substrates. | when grown on formate, formate-co, and methanol-co, butyribacterium methylotrophicum contained high levels of tetrahydrofolate (h4folate) and required enzymes, carbon monoxide dehydrogenase, formate dehydrogenase, and hydrogenase. the activities of methylene-h4folate reductase were comparable to other h4 folate activities (which ranged from 0.55 to 9.28 mumol/min per mg of protein) when measured by an improved procedure. the h4folate activities in formate-grown cells were twice those found in fo ... | 1987 | 3316188 |
| a putative internal promoter in the 16 s/23 s intergenic spacer of the rrna operon of archaebacteria and eubacteria. | the existence of the internal promoter pi in the 16 s/23 s intergenic spacers of the rrna operons of an eubacterium escherichia coli and archaebacterium halobacterium halobium is proposed. the possible functional significance of these promoters is discussed. | 1987 | 3301400 |