| [species diversity of bifidobacteria in the intestinal microbiota studied using maldi-tof mass-spectrometry]. | the members of genus bifidobacterium represent a significant part of intestinal microbiota in adults and predominate in infants. species repertoire of the intestinal bifidobacteria is known to be subjected to major changes with age; however, many details of this process are still to be elucidated. | 2015 | 26710526 |
| beneficial effects of probiotic bifidobacterium and galacto-oligosaccharide in patients with ulcerative colitis: a randomized controlled study. | background/aim: in previous studies, we described the beneficial effects of bifidobacteria-fermented milk in patients with ulcerative colitis (uc). here, we examined the effects of a live bifidobacterium breve strain yakult, a probiotic contained in bifidobacteria-fermented milk, and galacto-oligosaccharide (gos) as synbiotics in uc patients. methods: forty-one patients with mild to moderate uc were assigned to two groups; one group was treated with the synbiotics and the other was not (control) ... | 2011 | 21525768 |
| cholesterol-lowering probiotics: in vitro selection and in vivo testing of bifidobacteria. | thirty-four strains of bifidobacteria belonging to bifidobacterium adolescentis, bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium longum, and bifidobacterium pseu-docatenulatum were assayed in vitro for the ability to assimilate cholesterol and for bile salt hydrolase (bsh) against glycocholic and taurodeoxycholic acids (gca and tdca). cholesterol assimilation was peculiar characteristic of two strains belonging to the species b. bifidum (b. bifidum mb 10 ... | 2013 | 23872958 |
| isolation of a bifidogenic peptide from the pepsin hydrolysate of bovine lactoferrin. | lactoferrin is an iron-binding glycoprotein found in the milk of most mammals for which various biological functions have been reported, such as antimicrobial activity and bifidogenic activity. in this study, we compared the bifidogenic activity of bovine lactoferrin (blf) and pepsin hydrolysate of blf (blfh), isolated bifidogenic peptide from blfh, and investigated the bifidogenic spectra of blf, blfh, and its active peptide against 42 bifidobacterial strains comprising nine species. against bi ... | 2013 | 23315725 |
| production and evaluation of dry alginate-chitosan microcapsules as an enteric delivery vehicle for probiotic bacteria. | this study investigates the production of alginate microcapsules, which have been coated with the polysaccharide chitosan, and evaluates some of their properties with the intention of improving the gastrointestinal viability of a probiotic ( bifidobacterium breve ) by encapsulation in this system. the microcapsules were dried by a variety of methods, and the most suitable was chosen. the work described in this article is the first report detailing the effects of drying on the properties of these ... | 2011 | 21574635 |
| analysis of infant isolates of bifidobacterium breve by comparative genome hybridization indicates the existence of new subspecies with marked infant specificity. | a total of 20 bifidobacterium strains were isolated from fecal samples of 4 breast- and bottle-fed infants and all were characterized as bifidobacterium breve based on 16s rrna gene sequence and metabolic analysis. these isolates were further characterized and compared to the type strains of b. breve and 7 other bifidobacterium spp. by comparative genome hybridization. for this purpose, we constructed and used a dna-based microarray containing over 2000 randomly cloned dna fragments from b. brev ... | 2011 | 21726634 |
| toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: finding new anti-inflammatory immunobiotics. | a total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the nf-+¦b-reporter assay using a toll-like receptor 2-expressing transfectant (hek(ptlr2) system) and the mitogenic assay using porcine peyer's patches immunocompetent cells. among the four preselected strains from different immunomodulatory groups, bifidobacterium breve mcc-117 was able to efficiently modulate the inflammatory ... | 2011 | 21711398 |
| comparative genomics revealed genetic diversity and species/strain-level differences in carbohydrate metabolism of three probiotic bifidobacterial species. | strains of bifidobacterium longum, bifidobacterium breve, and bifidobacterium animalis are widely used as probiotics in the food industry. although numerous studies have revealed the properties and functionality of these strains, it is uncertain whether these characteristics are species common or strain specific. to address this issue, we performed a comparative genomic analysis of 49 strains belonging to these three bifidobacterial species to describe their genetic diversity and to evaluate spe ... | 2015 | 26236711 |
| bifidobacterium breve - ht-29 cell line interaction: modulation of tnf-+¦ induced gene expression. | to provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of bifidobacterium breve. to select an appropriate test system reflecting inflammatory conditions, the responsiveness to tnf-+¦ was compared in t84, caco-2 and ht-29 cells. the highest tnf-+¦ response was observed in ht-29 cells and this cell line was selected for exposure to the b. breve strain ... | 2011 | 21831793 |
| mupirocin-mucin agar for selective enumeration of bifidobacterium bifidum. | bifidobacterium bifidum is a bacterial species exclusively found in the human intestinal tract. this species is becoming increasingly popular as a probiotic organism added to lyophilized products. in this study, porcine mucin was used as the sole carbon source for the selective enumeration of b. bifidum in probiotic food additives. thirty-six bifidobacterial strains were cultivated in broth with mucin. only 13 strains of b. bifidum utilized the mucin to produce acids. b. bifidum was selectively ... | 2014 | 25217723 |
| bile enhances cell surface hydrophobicity and biofilm formation of bifidobacteria. | twenty-four human bifidobacterial strains were analysed for cell surface hydrophobicity (csh) using a salt aggregation test (sat) and a congo red binding (crb) assay. three strains were selected for a systematic study on the csh and biofilm formation: bifidobacterium breve 46, bifidobacterium animalis ssp. lactis 8:8 and a reference strain b. animalis ssp. lactis jcm 10602. crb of the b. breve 46 and b. animalis ssp. lactis jcm 10602 was significantly enhanced (p < 0.05) when grown in deman-rogo ... | 2014 | 24318587 |
| beneficial microbes for premature infants, and children with malignancy undergoing chemotherapy. | this review reports the beneficial effects, observed in our clinical studies, of bifidobacterium breve for premature infants, and children with cancers undergoing chemotherapy. to investigate the protective effects of b. breve (m-16v) as a probiotic on necrotizing enterocolitis (nec) and infection in premature infants, we carried out a clinical study in 338 very low birth weight infants over a five-year period. these patients were supplemented with b. breve starting several hours after birth (bi ... | 2010 | 21831775 |
| in vitro fermentation of caprine milk oligosaccharides by bifidobacteria isolated from breast-fed infants. | this study was conducted to investigate the catabolism and fermentation of caprine milk oligosaccharides (cmo) by selected bifidobacteria isolated from 4 breast-fed infants. seventeen bifidobacterial isolates consisting of 3 different species (bifidobacterium breve, bifidobacterium longum subsp. longum and bifidobacterium bifidum) were investigated. a cmo-enriched fraction (cmof) (50% oligosaccharides, 10% galacto-oligosaccharides (gos), 20% lactose, 10% glucose and 10% galactose) from caprine c ... | 2015 | 26587678 |
| commensal and probiotic bacteria influence intestinal barrier function and susceptibility to colitis in nod1(-/-) ;nod2(-/-) mice. | background: the intestinal microbiota regulates key host functions. it is unknown whether modulation of the microbiota can affect a genetically determined host phenotype. polymorphisms in the nucleotide oligomerization domain (nod)-like receptor family confer genetic risk for inflammatory bowel disease (ibd). we investigated whether the intestinal microbiota and the probiotic strain bifidobacterium breve ncc2950 affect intestinal barrier function and responses to intestinal injury in nod1(-/-) ; ... | 2011 | 22162005 |
| ability of bifidobacterium breve to grow on different types of milk: exploring the metabolism of milk through genome analysis. | we have investigated the occurrence of bifidobacteria in human milk samples, and we provide evidence regarding the predominance of members of the bifidobacterium breve species in this environment. moreover, evaluation of the growth capabilities and transcriptomic analyses of one representative isolate of this species, i.e., b. breve 4l, on different milk types were performed. | 2011 | 21856831 |
| multilocus sequence typing of bifidobacterial strains from infant's faeces and human milk: are bifidobacteria being sustainably shared during breastfeeding? | bifidobacteria are considered to be one of the most important beneficial intestinal bacteria for infants, contributing to the priming of the mucosal immune system. these microbes can also be detected in mother's milk, suggesting a potential role of human milk in the colonisation of infant's gut. however, little is known about the timing of bacteria appearance in human milk, and whether human milk is the first source of inoculation. here, we investigated whether specific strains are shared sustai ... | 2015 | 25691099 |
| genome sequence of bifidobacterium breve dpc 6330, a strain isolated from the human intestine. | the draft genome of bifidobacterium breve dpc 6330, isolated from an elderly patient, was determined. b. breve dpc 6330 was previously identified to synthesize the beneficial metabolite conjugated linoleic acid from free linoleic acid. the sequence will allow identification and characterization of the genetic determinants of its putative beneficial properties. | 2011 | 22072651 |
| discovering novel bile protection systems in bifidobacterium breve ucc2003 through functional genomics. | tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. a transcriptomic approach was employed to study the response of bifidobacterium breve ucc2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. the potential role of a selection of these bile-inducible genes in bile protection was analyzed following heterologous expression in lactococcus lactis. g ... | 2011 | 22156415 |
| variation in consumption of human milk oligosaccharides by infant gut-associated strains of bifidobacterium breve. | human milk contains a high concentration of complex oligosaccharides that influence the composition of the intestinal microbiota in breast-fed infants. previous studies have indicated that select species such as bifidobacterium longum subsp. infantis and bifidobacterium bifidum can utilize human milk oligosaccharides (hmo) in vitro as the sole carbon source, while the relatively few b. longum subsp. longum and bifidobacterium breve isolates tested appear less adapted to these substrates. conside ... | 2013 | 23892749 |
| Identification of iron-regulated genes of Bifidobacterium breve UCC2003 as a basis for controlled gene expression. | Iron is an essential growth factor for virtually all organisms. However, iron is not readily available in most environments and microorganisms have evolved specialized mechanisms, such as the use of siderophores and high-affinity transport systems, to acquire iron when confronted with iron-limiting conditions. In general these systems are tightly regulated to prevent iron-induced toxicity and because they are quite costly to the microbe. Because of this tight regulation we chose to explore the r ... | 2012 | 22179149 |
| no detectable beneficial systemic immunomodulatory effects of a specific synbiotic mixture in infants with atopic dermatitis. | background: in a murine model of allergic inflammation, bifidobacterium breve m-16v has been shown to reduce il-4 and ige by inducing il-10 and ifn-γ. however, it remains unknown whether this strain has the same effect in humans with allergic disease. objective: to determine the effects of bifidobacterium breve m-16v combined with a prebiotic oligosaccharide mixture (synbiotic) on atopic markers, ex vivo cytokine production by peripheral blood mononuclear cells (pbmcs) and circulating regulatory ... | 2011 | 22092915 |
| cross-feeding by bifidobacterium breve ucc2003 during co-cultivation with bifidobacterium bifidum prl2010 in a mucin-based medium. | bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. bifidobacterium breve ucc2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and hmo-derived monosaccharide, sialic acid. in the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprot ... | 2014 | 25420416 |
| identification and characterization of an oleate hydratase-encoding gene from bifidobacterium breve. | bifidobacteria are common commensals of the mammalian gastrointestinal tract. previous studies have suggested that a bifidobacterial myosin cross reactive antigen (mcra) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (cla) in bifidobacteria. in order to increase our understanding on the role of mcra in bifidobacteria we created and analyzed an insertion mutant of the mcra-encoding gene of b. breve ncfb 2 ... | 2013 | 23851389 |
| cephalosporinases associated with outer membrane vesicles released by bacteroides spp. protect gut pathogens and commensals against β-lactam antibiotics. | to identify β-lactamase genes in gut commensal bacteroides species and to assess the impact of these enzymes, when carried by outer membrane vesicles (omvs), in protecting enteric pathogens and commensals. | 2014 | 25433011 |
| evaluation of potential probiotics isolated from human milk and colostrum. | several studies have demonstrated a diversity of bacterial species in human milk, even in aseptically collected samples. the present study evaluated potential probiotic bacteria isolated from human milk and associated maternal variables. milk samples were collected from 47 healthy women and cultured on selective and universal agar media under aerobic and anaerobic conditions. bacterial isolates were counted and identified by biotyper matrix-assisted laser desorption ionization-time of flight mas ... | 2017 | 28374172 |
| a conserved two-component signal transduction system controls the response to phosphate starvation in bifidobacterium breve ucc2003. | this work reports on the identification and molecular characterization of the two-component regulatory system (2crs) phorp, which controls the response to inorganic phosphate (p(i)) starvation in bifidobacterium breve ucc2003. the response regulator phop was shown to bind to the promoter region of pstscab, specifying a predicted p(i) transporter system, as well as that of phou, which encodes a putative p(i)-responsive regulatory protein. this interaction is assumed to cause transcriptional modul ... | 2012 | 22635988 |
| ribose utilization by the human commensal bifidobacterium breve ucc2003. | growth of bifidobacterium breve ucc2003 on ribose leads to the transcriptional induction of the rbsacbdk gene cluster. generation and phenotypic analysis of an rbsa insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a laci-type regulator encoded by rbsr, located immediately upstream of rbsa. gel mobility shift assays using purified rbsr(his) indicate that the promoter upstream of rbsabcdk is negatively con ... | 2010 | 21255330 |
| protective effect of a synbiotic against multidrug-resistant acinetobacter baumannii in a murine infection model. | this study investigated the ability of the probiotic bifidobacterium breve strain yakult (bby) to protect against infection, as well as the potentiation of bby activity by the synbiotic combination of bby and prebiotic galactooligosaccharides (gos). the study employed a mouse model of lethal intestinal multidrug-resistant acinetobacter baumannii (mdrab) infection. the endogenous intestinal microbiota was disrupted by the administration of multiple antibiotics, causing the loss of endogenous bifi ... | 2016 | 26953197 |
| enhancement of ginsenoside rg(1) in panax ginseng hairy root by overexpressing the α-l-rhamnosidase gene from bifidobacterium breve. | to improve the production of ginsenoside rg1 in panax ginseng. | 2015 | 26087948 |
| trophic interactions of infant bifidobacteria and eubacterium hallii during l-fucose and fucosyllactose degradation. | fucosyllactoses (2'- or 3'-fl) account for up to 20% of human milk oligosaccharides (hmos). infant bifidobacteria, such as bifidobacterium longum subsp. infantis, utilize the lactose moiety to form lactate and acetate, and metabolize l-fucose to 1,2-propanediol (1,2-pd). eubacterium hallii is a common member of the adult gut microbiota that can produce butyrate from lactate and acetate, and convert 1,2-pd to propionate. recently, a swiss cohort study identified e. hallii as one of the first buty ... | 2017 | 28194144 |
| is bifidobacterium breve effective in the treatment of childhood constipation? results from a pilot study. | probiotics are increasingly used in the treatment of functional gastrointestinal disorders. studies in constipated adults with a bifidus yoghurt (containing bifidobacterium breve, bifidobacterium bifidum and lactobacillus acidophilus) showed a significant increase in defecation frequency. the aim of this pilot study was to determine if bifidobacterium breve is effective in the treatment of childhood constipation. | 2011 | 21345213 |
| bacillus subtilis natto: a non-toxic source of poly-γ-glutamic acid that could be used as a cryoprotectant for probiotic bacteria. | it is common practice to freeze dry probiotic bacteria to improve their shelf life. however, the freeze drying process itself can be detrimental to their viability. the viability of probiotics could be maintained if they are administered within a microbially produced biodegradable polymer - poly-γ-glutamic acid (γ-pga) - matrix. although the antifreeze activity of γ-pga is well known, it has not been used for maintaining the viability of probiotic bacteria during freeze drying. the aim of this s ... | 2013 | 23829836 |
| a fermented milk concentrate and a combination of short-chain galacto-oligosaccharides/long-chain fructo-oligosaccharides/pectin-derived acidic oligosaccharides protect suckling rats from rotavirus gastroenteritis. | human milk contains bioactive compounds that confer a protective role against gastrointestinal infections. in order to find supplements for an infant formula able to mimic these benefits of breast-feeding, two different concepts were tested. the products consisted of the following: (1) a bifidobacterium breve- and streptococcus thermophilus-fermented formula and (2) a combination of short-chain galacto-oligosaccharides/long-chain fructo-oligosaccharides with pectin-derived acidic oligosaccharide ... | 2017 | 28166850 |
| a specific mixture of fructo-oligosaccharides and bifidobacterium breve m-16v facilitates partial non-responsiveness to whey protein in mice orally exposed to β-lactoglobulin-derived peptides. | oral tolerance is a promising approach for allergy prevention in early life, but it strongly depends on allergen exposure and proper immune environment. small tolerance-inducing peptides and dietary immunomodulatory components may comprise an attractive method for allergy prevention in at-risk infants. this study aimed to investigate whether early oral exposure to β-lactoglobulin-derived peptides (blg-peptides) and a specific synbiotic mixture of short- and long- chain fructo-oligosaccharides (s ... | 2016 | 28127297 |
| bifidobacterium breve reduces apoptotic epithelial cell shedding in an exopolysaccharide and myd88-dependent manner. | certain members of the microbiota genus bifidobacterium are known to positively influence host well-being. importantly, reduced bifidobacterial levels are associated with inflammatory bowel disease (ibd) patients, who also have impaired epithelial barrier function, including elevated rates of apoptotic extrusion of small intestinal epithelial cells (iecs) from villi-a process termed 'cell shedding'. using a mouse model of pathological cell shedding, we show that mice receiving bifidobacterium br ... | 2017 | 28123052 |
| effect of bifidobacterium breve on the intestinal microbiota of coeliac children on a gluten free diet: a pilot study. | coeliac disease (cd) is associated with alterations of the intestinal microbiota. although several bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to cd subjects. this study evaluated the effect of administration for three months of a food supplement based on two bifidobacterium breve strains (b632 and br03) to restore the gut microbial balance in coeliac children on ... | 2016 | 27782071 |
| molecular monitoring of the development of intestinal microbiota in japanese infants. | the faecal microbiota of 166 healthy japanese newborns was analysed periodically from day 1 after birth until the age of 3 years by using the reverse transcription-quantitative pcr. faecal ph and the organic acid concentration were also examined. colonisation by both facultative anaerobes and strict anaerobes was confirmed in 95% of the meconium tested. bifidobacterium-predominant microbiota was established subsequently in most of the infants by 3 months after birth. bifidobacterium breve, bifid ... | 2012 | 22683836 |
| monoculture parameters successfully predict coculture growth kinetics of bacteroides thetaiotaomicron and two bifidobacterium strains. | microorganisms rarely live in isolation but are most often found in a consortium. this provides the potential for cross-feeding and nutrient competition among the microbial species, which make it challenging to predict the growth kinetics in coculture. in this paper we developed a mathematical model to describe substrate consumption and subsequent microbial growth and metabolite production for bacteria grown in monoculture. the model characterized substrate utilization kinetics of 18 bifidobacte ... | 2014 | 25282609 |
| glycan cross-feeding activities between bifidobacteria under in vitro conditions. | bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. the glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with different glycan-degrading properties. in this study, we observed an enhanced growth yield of bifidobacterium bifidum prl2010 when co-cultivated with bifidobacterium breve 12l, bifid ... | 2015 | 26441950 |
| cloning and characterization of ginsenoside ra1-hydrolyzing beta-d-xylosidase from bifidobacterium breve k-110. | beta-d-xylosidase (e.c. 3.2.1.37) from bifidobacterium breve k-110, which hydrolyzes ginsenoside ra1 to ginsenoside rb2, was cloned and expressed in escherichia coli. the (his6)-tagged recombinant enzyme, designated as xlybk- 110, was efficiently purified using ni²⁺-affinity chromatography (109.9-fold, 84% yield). the molecular mass of xylbk- 100 was found to be 55.7 kda by sds-page. its sequence revealed a 1,347 bp open reading frame (orf) encoding a protein containing 448 amino acids, which sh ... | 2012 | 22534302 |
| effect of bifidobacterium upon clostridium difficile growth and toxicity when co-cultured in different prebiotic substrates. | the intestinal overgrowth of clostridium difficile, often after disturbance of the gut microbiota by antibiotic treatment, leads to c. difficile infection (cdi) which manifestation ranges from mild diarrhea to life-threatening conditions. the increasing cdi incidence, not only in compromised subjects but also in traditionally considered low-risk populations, together with the frequent relapses of the disease, has attracted the interest for prevention/therapeutic options. among these, probiotics, ... | 2016 | 27242753 |
| prebiotic preferences of human lactobacilli strains in co-culture with bifidobacteria and antimicrobial activity against clostridium difficile. | to evaluate robustness, prebiotic utilization of lactobacillus paracasei f8 and lactobacillus plantarum f44 in mono- and co-cultures with bifidobacterium breve 46 and bifidobacterium animalis sub sp. lactis 8 : 8 and antimicrobial activity of co-culture against clostridium difficile. | 2015 | 26381324 |
| bifidobacterium breve c50 secretes lipoprotein with chap domain recognized in aggregated form by tlr2. | extracellular components secreted by bifidobacterium breve c50 can induce maturation, high il-10 production and prolonged survival of dendritic cells via a tlr2 pathway. in this study, the components were isolated from the supernatant by gel filtration chromatography. antibodies raised against the major compounds with molecular weight above 600 kda (bb c50bc) also recognized compounds of lower molecular weight (200–600 kda). tlr2 and tlr6 bound to the components already recognized by the antibod ... | 2014 | 25457102 |
| a probiotic combination attenuates experimental colitis through inhibition of innate cytokine production. | inflammatory bowel disease (ibd) is a severe immune cell-mediated syndrome characterised by extensive inflammatory and effector mucosal responses leading to tissue destruction in the colon and small intestine. the leading hypothesis is that dysbiosis of the gut flora causes an excessive immune response and inflammation in the gastrointestinal track. lactic acid bacteria (lab) can correct dysbiosis of the normal microbiota. in the current study, the therapeutic potential of seven lab strains in c ... | 2016 | 28008786 |
| in vitro evaluation of bifidobacterium strains of human origin for potential use in probiotic functional foods. | the present study investigated some in vitro properties for probiotic use of four strains of bifidobacteria isolated from faeces of healthy children (bifidobacterium longum 51a, bifidobacterium breve 1101a, bifidobacterium pseudolongum 1191a and bifidobacterium bifidum 1622a). in vitro tests were carried out to compare growth rate, aerotolerance, antagonistic activity against pathogens, antimicrobial susceptibility profile and cell wall hydrophobicity. mean doubling time of b. longum 51a was sho ... | 2013 | 23443950 |
| interaction of bifidobacterium bifidum lmg13195 with ht29 cells influences regulatory-t-cell-associated chemokine receptor expression. | probiotics play an important role in the maintenance of the gastrointestinal barrier. in addition to direct effects on mucosal integrity, the interaction with the intestinal mucosa may have an active immunoregulatory effect. in the present work, we exposed ht29 intestinal epithelial cells to two bifidobacterium species to determine their effect on gene expression profile, enterocyte monolayer integrity, and t-cell response. bifidobacterium breve ipla 20004 triggered a more pronounced increase in ... | 2012 | 22344636 |
| probiotics and antimicrobial protein and peptide levels in preterm infants. | to characterise the secreted and inducible antimicrobial protein and peptide (app) levels in a prospective cohort of preterm infants (<30 weeks gestational age) with or without bifidobacterium breve m16v supplementation during the first month of life. | 2017 | 28294428 |
| bifidobacterium breve with α-linolenic acid alters the composition, distribution and transcription factor activity associated with metabolism and absorption of fat. | this study focused on the mechanisms that fatty acid conjugating strains - bifidobacterium breve ncimb 702258 and bifidobacterium breve dpc 6330 - influence lipid metabolism when ingested with α-linolenic acid (ala) enriched diet. four groups of balb/c mice received ala enriched diet (3% (w/w)) either alone or in combination with b. breve ncimb 702258 or b. breve dpc 6330 (10(9) cfu/day) or unsupplemented control diet for six weeks. the overall n-3 pufa score was increased in all groups receivin ... | 2017 | 28265110 |
| the neonatal gut harbours distinct bifidobacterial strains. | recent studies have described the bifidobacterial composition of neonates at a species level; however, with advancing technologies we can gain insight into the diversity of the bifidobacterial microbiota residing within the infant gut. | 2015 | 25896967 |
| a novel lactobacillus casei lp1 producing 1,4-dihydroxy-2-naphthoic acid, a bifidogenic growth stimulator. | 1,4-dihydroxy-2-naphthoic acid (dhna) is a bifidogenic growth stimulator (bgs) and could be a functional food ingredient since bifidobacteria are beneficial for human health. for that reason, lactic acid bacteria producing dhna have been screened. a lactic acid bacterium lp1 strain isolated from a natural cheese was confirmed to produce dhna, analyzed by a hplc method. the strain was identified as lactobacillus casei by 16s rrna gene sequence analysis. the cell-free supernatant of fermented whey ... | 2015 | 25866754 |
| probiotic bifidobacterium species stimulate human slc26a3 gene function and expression in intestinal epithelial cells. | slc26a3, or downregulated in adenoma (dra), plays a major role in mediating cl(-) absorption in the mammalian intestine. disturbances in dra function and expression have been implicated in intestinal disorders such as congenital cl(-) diarrhea and gut inflammation. we previously showed that an increase in dra function and expression by lactobacillus acidophilus and its culture supernatant (cs) might underlie antidiarrheal effects of this probiotic strain. however, the effects of bifidobacterium ... | 2014 | 25143346 |
| metabolism of sialic acid by bifidobacterium breve ucc2003. | bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. bifidobacterium breve ucc2003 has previously been shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. in the present study, we investigated the ability of this strain to utilize the mucin- and human milk oligosaccharide (hmo)-derived carbohydrate sialic acid. using a combination of transcriptomic and functional g ... | 2014 | 24814790 |
| comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk. | the aim of the study was to compare the compositions of the fecal microbiotas of infants fed goat milk formula to those of infants fed cow milk formula or breast milk as the gold standard. pyrosequencing of 16s rrna gene sequences was used in the analysis of the microbiotas in stool samples collected from 90 australian babies (30 in each group) at 2 months of age. beta-diversity analysis of total microbiota sequences and lachnospiraceae sequences revealed that they were more similar in breast mi ... | 2013 | 23455335 |
| assessment of intestinal microbiota modulation ability of bifidobacterium strains in in vitro fecal batch cultures from preterm neonates. | microbial colonization of the infant gut is essential for the development of the intestine and the immune system. the intestinal microbiota of full-term breast-fed infants is considered as the health standard for newborns. a culture medium containing formula milk was designed, which allowed a balanced growth of intestinal microorganisms and was used to perform fecal batch cultures from preterm babies. sixteen bifidobacterium strains and fructooligosaccharides (fos) were tested for their ability ... | 2013 | 23154045 |
| conjugated linoleic and linolenic acid production kinetics by bifidobacteria differ among strains. | there is great interest in conjugated linoleic acid (cla) and conjugated linolenic acid (clna) isomers because of their supposed health-promoting properties. therefore, the differences in production kinetics of cla and clna isomers from linoleic acid (la) and α-linolenic acid (α-lna), respectively, by bifidobacteria were investigated. laboratory fermentations, supplemented with la or α-lna in the fermentation medium, were performed with bifidobacterium bifidum lmg 10645, bifidobacterium breve lm ... | 2012 | 22405353 |
| prebiotic effects of bovine lactoferrin on specific probiotic bacteria. | bovine lactoferrin (blf) is a natural iron-binding protein and it has been suggested to be a prebiotic agent, but this finding remains inconclusive. this study explores the prebiotic potential of blf in 14 probiotics. initially, blf (1-32 mg/ml) treatment showed occasional and slight prebiotic activity in several probiotics only during the late experimental period (48, 78 h) at 37 °c. we subsequently supposed that blf exerts stronger prebiotic effects when probiotic growth has been temperately r ... | 2017 | 28185076 |
| a methodological framework for assessing agreement between cost-effectiveness outcomes estimated using alternative sources of data on treatment costs and effects for trial-based economic evaluations. | a new methodological framework for assessing agreement between cost-effectiveness endpoints generated using alternative sources of data on treatment costs and effects for trial-based economic evaluations is proposed. the framework can be used to validate cost-effectiveness endpoints generated from routine data sources when comparable data is available directly from trial case report forms or from another source. we illustrate application of the framework using data from a recent trial-based econ ... | 2017 | 28180977 |
| fast screening of antibacterial compounds from fusaria. | bio-guided screening is an important method to identify bioactive compounds from fungi. in this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus fusarium. here antibacterial effects could be detected for antibiotic y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. the system was then used in a bio-guided screen of extracts from 14 different fusarium spe ... | 2016 | 27916854 |
| bacteremia induced by bifidobacterium breve in a newborn with cloacal exstrophy. | bifidobacterium breve is an effective probiotic agent used in the field of neonatology. although b. breve has been considered safe, a case of b. breve bacteremia has been reported. the pathogenic mechanism underlying the bacteremia is unknown. herein, we report a second case of b. breve bacteremia that developed in a neonate with multiple abdominal organ anomalies. following surgical repair immediately after birth, b. breve treatment was started. after 1 week, the infant developed b. breve bacte ... | 2016 | 27882741 |
| the effect of oral consumption of probiotics in prevention of heart injury in a rat myocardial infarction model: a histopathological, hemodynamic and biochemical evaluation. | despite the emerging evidence on beneficial effects of probiotics on the cardiovascular system, their impact on the management of ischemic heart diseases and its possible mechanism have not been elucidated. | 2016 | 27874107 |
| preclinical immunomodulation by the probiotic bifidobacterium breve m-16v in early life. | this study aimed to investigate the effect of supplementation with the probiotic bifidobacterium breve m-16v on the maturation of the intestinal and circulating immune system during suckling. in order to achieve this purpose, neonatal lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. the animals were weighed during the study, and faecal samples were obtained and evaluated daily. on day 19, rats were euthanized and intestinal wash samples, mesenteric lym ... | 2016 | 27820846 |
| mechanistic study of utilization of water-insoluble saccharomyces cerevisiae glucans by bifidobacterium breve strain jcm1192. | bifidobacteria exert beneficial effects on hosts and are extensively used as probiotics. however, due to the genetic inaccessibility of these bacteria, little is known about their mechanisms of carbohydrate utilization and regulation. bifidobacterium breve strain jcm1192 can grow on water-insoluble yeast (saccharomyces cerevisiae) cell wall glucans (ycwg), which were recently considered as potential prebiotics. according to the results of (1)h nuclear magnetic resonance (nmr) spectrometry, the y ... | 2017 | 28115383 |
| glycoside hydrolase family 13 α-glucosidases encoded by bifidobacterium breve ucc2003; a comparative analysis of function, structure and phylogeny. | bifidobacterium breve is a noted inhabitant and one of the first colonizers of the human gastro intestinal tract (git). the ability of this bacterium to persist in the git is reflected by the abundance of carbohydrate-active enzymes that are encoded by its genome. one such family of enzymes is represented by the α-glucosidases, of which three, agl1, agl2 and meld, have previously been identified and characterized in the prototype b. breve strain ucc2003. in this report, we describe an additional ... | 2016 | 26967000 |
| benefits of bifidobacterium breve m-16v supplementation in preterm neonates - a retrospective cohort study. | systematic reviews of randomised controlled trials report that probiotics reduce the risk of necrotising enterocolitis (nec) in preterm neonates. | 2016 | 26953798 |
| antagonistic activities of some bifidobacterium sp. strains isolated from resident infant gastrointestinal microbiota on gram-negative enteric pathogens. | the gastrointestinal microbiota contributes to the consolidation of the anti-infectious barrier against enteric pathogens. the purpose of this study was to investigate the influence of bifidobacterium sp. strains, recently isolated from infant gastrointestinal microbiota on the in vitro growth and virulence features expression of enteropathogenic bacterial strains. the antibacterial activity of twelve bifidobacterium sp. strains isolated from human feces was examined in vitro against a wide rang ... | 2016 | 26921694 |
| probiotic streptococcus thermophilus fp4 and bifidobacterium breve br03 supplementation attenuates performance and range-of-motion decrements following muscle damaging exercise. | probiotics have immunomodulatory effects. however, little is known about the potential benefit of probiotics on the inflammation subsequent to strenuous exercise. in a double-blind, randomized, placebo controlled, crossover design separated by a 21-day washout, 15 healthy resistance-trained men ingested an encapsulated probiotic streptococcus (s.) thermophilus fp4 and bifidobacterium (b.) breve br03 at 5 bn live cells (afu) concentration each, or a placebo, daily for 3 weeks prior to muscle-dama ... | 2016 | 27754427 |
| the association of bifidobacterium breve br03 and b632 is effective to prevent colics in bottle-fed infants: a pilot, controlled, randomized, and double-blind study. | to assess the effectiveness of bifidobacterium breve b632 and br03 association in the reduction of infants crying over time. the second endpoint was to observe the effect of the same strains on daily evacuations and on the number of regurgitations and vomits. | 2016 | 27741166 |
| can probiotics reduce diarrhea and infant mortality in africa?: the project of a pilot study. | diarrhea accounts for 9% of the mortality among children under 5 years of age worldwide, and it is significantly associated with malnutrition. each year, diarrhea kills around 760,000 children under 5 years of age and most of these are in sub-saharan africa.in uganda, the infant mortality rate of 58 per 1000 is unacceptably high, and the major contributors include malnutrition, diarrhea, pneumonia, malaria, prematurity, sepsis, and newborn illnesses.there is an urgent need for intervention to pr ... | 2016 | 27741153 |
| glycosulfatase-encoding gene cluster in bifidobacterium breve ucc2003. | bifidobacteria constitute a specific group of commensal bacteria typically found in the gastrointestinal tract (git) of humans and other mammals. bifidobacterium breve strains are numerically prevalent among the gut microbiota of many healthy breastfed infants. in the present study, we investigated glycosulfatase activity in a bacterial isolate from a nursling stool sample, b. breve ucc2003. two putative sulfatases were identified on the genome of b. breve ucc2003. the sulfated monosaccharide n- ... | 2016 | 27590817 |
| screening of immune-active lactic acid bacteria. | the purpose of this study was to investigate the effect of lactic acid bacteria (lab) cell wall extract on the proliferation and cytokine production of immune cells to select suitable probiotics for space food. ten strains of lab (lactobacillus bulgaricus, l. paracasei, l. casei, l. acidophilus, l. plantarum, l. delbruekii, lactococcus lactis, streptococcus thermophilus, bifidobacterium breve, and pedicoccus pentosaceus) were sub-cultured and further cultured for 3 d to reach 7-10 log colony-for ... | 2015 | 26761877 |
| in vitro and in vivo evaluation of the prebiotic effect of raw and roasted almonds (prunus amygdalus). | almonds contain considerable amounts of potential prebiotic components, and the roasting process may alter these components. the aim of this study was to compare the in vitro fermentation properties and in vivo prebiotic effect of raw and roasted almonds. | 2016 | 26749248 |
| interaction of mouse splenocytes and macrophages with bacterial strains in vitro: the effect of age in the immune response. | probiotics influence the immune system, both at the local and systemic level. recent findings suggest the relation between microbiota and the immune system alters with age. our objective was to address direct effects of six bacterial strains on immune cells from young and aged mice: lactobacillus plantarum wcfs1, lactobacillus casei bl23, lactococcus lactis mg1363, bifidobacterium breve atcc15700, bifidobacterium infantis atcc15697, and akkermansia muciniphila atcc baa-835. we used splenocytes a ... | 2016 | 26689225 |
| supplementation with lactobacillus plantarum wcfs1 prevents decline of mucus barrier in colon of accelerated aging ercc1(-/δ7) mice. | although it is clear that probiotics improve intestinal barrier function, little is known about the effects of probiotics on the aging intestine. we investigated effects of 10-week bacterial supplementation of lactobacillus plantarum wcfs1, lactobacillus casei bl23, or bifidobacterium breve dsm20213 on gut barrier and immunity in 16-week-old accelerated aging ercc1(-/δ7) mice, which have a median lifespan of ~20 weeks, and their wild-type littermates. the colonic barrier in ercc1(-/δ7) mice was ... | 2016 | 27774093 |
| the composition of human milk and infant faecal microbiota over the first three months of life: a pilot study. | human milk contains a diverse array of bioactives and is also a source of bacteria for the developing infant gut. the aim of this study was to characterize the bacterial communities in human milk and infant faeces over the first 3 months of life, in 10 mother-infant pairs. the presence of viable bifidobacterium and lactobacillus in human milk was also evaluated. miseq sequencing revealed a large diversity of the human milk microbiota, identifying over 207 bacterial genera in milk samples. the ph ... | 2017 | 28094284 |
| specific probiotic dietary supplementation leads to different effects during remission and relapse in murine chronic colitis. | although interest in using probiotics to prevent and treat intestinal diseases is increasing, the effects of specific probiotic strains still remain unclear. here, we assess the therapeutic effects of two probiotic strains, lactobacillus rhamnosus nutres 1 and bifidobacterium breve nutres 204 on a dextran sodium sulphate (dss)-induced chronic murine colitis model. the chronic colitis was induced by two dss treatment cycles with a rest period of 10 days (the remission or resolution phase). the pr ... | 2016 | 26645352 |
| bifidobacterium breve bbg-001 in very preterm infants: a randomised controlled phase 3 trial. | probiotics may reduce necrotising enterocolitis and late-onset sepsis after preterm birth. however, there has been concern about the rigour and generalisability of some trials and there is no agreement about whether or not they should be used routinely. we aimed to test the effectiveness of the probiotic bifidobacterium breve bbg-001 to reduce necrotising enterocolitis, late-onset sepsis, and death in preterm infants. | 2016 | 26628328 |
| effect of supplements: probiotics and probiotic plus honey on blood cell counts and serum iga in patients receiving pelvic radiotherapy. | radiotherapy is frequently used in treatment approaches of pelvic malignancies. nevertheless, it has some known systemic effects on blood cells and the immune system that possibly results in their susceptibility to infection. probiotics are live microbial food ingredients that provide a health advantage to the consumer. honey has prebiotic properties. the aim of this clinical trial was to investigate probable effects of probiotic or probiotics plus honey on blood cell counts and serum iga levels ... | 2015 | 26622258 |
| lysozyme in breast milk is a selection factor for bifidobacterial colonisation in the infant intestine. | the objective of this work was to study the residential characteristics of bifidobacteria, which can be classified as either human-residential bifidobacteria (hrb) or non-hrb. we investigated the growth of different strains of hrb and non-hrb in human breast milk with the aim of understanding the mechanisms involved in the unique habitation of each taxon. the growth of 37 strains of different bifidobacterial species or subspecies in breast milk was investigated by incubating each under anaerobic ... | 2016 | 26503736 |
| in silico mining and characterization of bifidobacterial lipoprotein with chap domain secreted in an aggregated form. | bifidobacterium breve c50 secretes a lipoprotein associated with glucose, acting in an aggregating form (>600kda) as an agonist of tlr2/6. similar lipoproteins were sought for in bifidobacteria. in silico, the closest homology was shown with a bifidobacterium longum protein containing chap and lipobox domains. two strains secreted aggregates whose peptides sequences aligned with the mined protein. c16:0 and c18:0 fatty acids detected in the aggregates further supported a lipoprotein structure. g ... | 2016 | 26459166 |
| effects of probiotics on biomarkers of oxidative stress and inflammatory factors in petrochemical workers: a randomized, double-blind, placebo-controlled trial. | the aim of the current study was to determine effects of probiotic yoghurt and multispecies probiotic capsule supplementation on biomarkers of oxidative stress and inflammatory factors in petrochemical workers. | 2015 | 26445629 |
| effects of bifidobacterium breve feeding strategy and delivery modes on experimental allergic rhinitis mice. | different delivery modes may affect the susceptibility to allergic diseases. it is still unknown whether early intervention with probiotics would counteract this effect. | 2015 | 26445348 |
| development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes. | the bovine intestinal epithelial cell line (bie cells) expresses the toll-like receptor (tlr)3 and is able to mount an antiviral immune response after the stimulation with poly(i:c). in the present study, we aimed to further characterise the antiviral defence mechanisms in bie cells by evaluating the innate immune response triggered by rotavirus (rv) infection. in addition, we attempted to determine whether immunobiotic bifidobacteria are able to confer protection of bie cells against rv infecti ... | 2017 | 28042704 |
| a multi-strain synbiotic may reduce viral respiratory infections in asthmatic children: a randomized controlled trial. | asthma is a growing problem worldwide. acute exacerbations impose considerable morbidity, mortality, and increased cost. viral respiratory infections are the most common cause (80-85%) of pediatric asthma exacerbations and admissions to the hospital. the aim of this study was to determine the effect of a new synbiotic lactocare® on viral respiratory infections and asthma exacerbations in asthmatic children. | 2016 | 27790333 |
| effect of administering a multi-species probiotic mixture on the changes in fecal microbiota and symptoms of irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. | we assessed the effect of multi-species probiotic mixture on the changes in fecal microbiota and irritable bowel syndrome (ibs) symptoms. eighty-one ibs patients were randomly assigned to receive either probiotic mixture (n = 39; containing lactobacillus acidophilus, l. rhamnosus, bifidobacterium breve, b. actis, b. longum, and streptococcus thermophilus) or placebo (n = 42) for 4 weeks. a questionnaire regarding general symptom relief was administered. the change in total symptom scores (sum of ... | 2015 | 26388670 |
| anti-inflammatory effects of lactobacillus rahmnosus and bifidobacterium breve on cigarette smoke activated human macrophages. | chronic obstructive pulmonary disease (copd) is a major global health problem with cigarette smoke (cs) as the main risk factor for its development. airway inflammation in copd involves the increased expression of inflammatory mediators such as cxcl-8 and il-1β which are important mediators for neutrophil recruitment. macrophages are an important source of these mediators in copd. lactobacillus rhamnosus (l. rhamnosus) and befidobacterium breve (b. breve) attenuate the development of 'allergic a ... | 2015 | 26317628 |
| bifidobacteria grown on human milk oligosaccharides downregulate the expression of inflammation-related genes in caco-2 cells. | breastfed human infants are predominantly colonized by bifidobacteria that thrive on human milk oligosaccharides (hmo). two predominant species of bifidobacteria in infant feces are bifidobacterium breve (b. breve) and bifidobacterium longum subsp. infantis (b. infantis), both of which include avid hmo-consumer strains. our laboratory has previously shown that b. infantis, when grown on hmo, increases adhesion to intestinal cells and increases the expression of the anti-inflammatory cytokine int ... | 2015 | 26303932 |
| bifidobacterium breve sepsis in child with high-risk acute lymphoblastic leukemia. | | 2015 | 26291071 |
| prebiotic effects of diet supplemented with the cultivated red seaweed chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites. | gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups. prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health. the red seaweed chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date. | 2015 | 26271359 |
| exploring vertical transmission of bifidobacteria from mother to child. | passage through the birth canal and consequent exposure to the mother's microbiota is considered to represent the initiating event for microbial colonization of the gastrointestinal tract of the newborn. however, a precise evaluation of such suspected vertical microbiota transmission has yet to be performed. here, we evaluated the microbiomes of four sample sets, each consisting of a mother's fecal and milk samples and the corresponding infant's fecal sample, by means of amplicon-based profiling ... | 2015 | 26231653 |
| complete genome sequence of bifidobacterium breve jcm 1192(t) isolated from infant feces. | bifidobacterium breve jcm 1192(t) was isolated from infant feces. here, we report the complete genome sequence of this organism. | 2015 | 26142063 |
| absence of adverse events in healthy individuals using probiotics--analysis of six randomised studies by one study group. | consumption of live bacteria as probiotic supplements is increasing. there is, however, a lack of information on the safety of ingested probiotics. the main objective of this study was to investigate the adverse events (aes) of specific probiotics (lactobacillus rhamnosus gg (lgg) alone or lgg in combination with l. rhamnosus lc705, propionibacterium freudenreichii js, bifidobacterium lactis bb12, or bifidobacterium breve 99) studied in six of our study groups' clinical trials, by analysing indi ... | 2016 | 26689224 |
| soluble bioactive microbial mediators regulate proteasomal degradation and autophagy to protect against inflammation-induced stress. | bifidobacterium breve and other gram-positive gut commensal microbes protect the gastrointestinal epithelium against inflammation-induced stress. however, the mechanisms whereby these bacteria accomplish this protection are poorly understood. in this study, we examined soluble factors derived from bifidobacterium breve and their impact on the two major protein degradation systems within intestinal epithelial cells, proteasomes and autophagy. conditioned media from gastrointestinal gram-positive, ... | 2016 | 27514476 |
| effect of probiotic and prebiotic fermented milk on skin and intestinal conditions in healthy young female students. | although persistent constipation is considered to be associated with skin problems, the supporting evidence is limited. hence, this study investigated the effects of probiotic and prebiotic fermented milk on skin and intestinal conditions in an open-label trial. among the 101 japanese healthy young female students that participated, the 81 subjects in the intake group consumed a bottle of probiotic and prebiotic fermented milk containing bifidobacterium breve strain yakult and galactooligosaccha ... | 2016 | 27508111 |
| activation of multiple chemotherapeutic prodrugs by the natural enzymolome of tumour-localised probiotic bacteria. | some chemotherapeutic drugs (prodrugs) require activation by an enzyme for efficacy. we and others have demonstrated the ability of probiotic bacteria to grow specifically within solid tumours following systemic administration, and we hypothesised that the natural enzymatic activity of these tumour-localised bacteria may be suitable for activation of certain such chemotherapeutic drugs. several wild-type probiotic bacteria; escherichia coli nissle, bifidobacterium breve, lactococcus lactis and l ... | 2016 | 26655063 |
| the role of probiotics in the treatment of dysentery: a randomized double-blind clinical trial. | diarrhea is considered as an important cause of morbidity and mortality, even though one of the main reasons of death following diarrhea is initiated by dysentery. in recent years, the consumption of probiotics has been proposed for the treatment of infectious diarrhea. despite most of the studies on probiotics have focused on acute watery diarrhea, few studies in the field of dysentery have found beneficial effects of probiotics. this study is a randomized double-blind clinical trial. the patie ... | 2017 | 28321826 |
| hypocholesterolemic effects of probiotic mixture on diet-induced hypercholesterolemic rats. | growing evidence has indicated that supplementation with probiotics improves lipid metabolism. we aimed to investigate the beneficial effects of a probiotics mixture (pm) of three strains belonging to the species bifidobacterium (b. longum, b. lactis, and b. breve) and two strains belonging to the species lactobacillus (l. reuteri and l. plantarum) on cholesterol-lowering efficacy in hypercholesterolemic rats. a hypercholesterolemic rat model was established by feeding a high-cholesterol diet fo ... | 2017 | 28300786 |
| preparative scale purification of fucosyl-n-acetylglucosamine disaccharides and their evaluation as potential prebiotics and antiadhesins. | fucosyl-n-acetylglucosamine disaccharides are important core structures that form part of human mucosal and milk glyco-complexes. we have previously shown that alfb and alfc α-l-fucosidases from lactobacillus casei are able to synthesize fucosyl-α-1,3--n-acetylglucosamine (fuc-α1,3-glcnac) and fucosyl-α-1,6-n-acetylglucosamine (fuc-α1,6-glcnac), respectively, in transglycosylation reactions. here, these reactions were performed in a semipreparative scale, and the produced disaccharides were puri ... | 2015 | 25977209 |
| maternal fucosyltransferase 2 status affects the gut bifidobacterial communities of breastfed infants. | individuals with inactive alleles of the fucosyltransferase 2 gene (fut2; termed the 'secretor' gene) are common in many populations. some members of the genus bifidobacterium, common infant gut commensals, are known to consume 2'-fucosylated glycans found in the breast milk of secretor mothers. we investigated the effects of maternal secretor status on the developing infant microbiota with a special emphasis on bifidobacterial species abundance. | 2015 | 25922665 |
| characterization of a bifidobacterial system that utilizes galacto-oligosaccharides. | the galacto-oligosaccharide (gos) oligomate 55n (yakult) is a mixture of oligosaccharides, the main component of which is 4'-galactosyllactose (4'-gl). numerous reports have shown that goss are non-digestible, reach the colon and selectively stimulate the growth of bifidobacteria. the product has been used as a food ingredient and its applications have expanded rapidly. however, the bifidobacterial glycoside hydrolases and transporters responsible for utilizing goss have not been characterized s ... | 2015 | 25903756 |
| synbiotics-supplemented amino acid-based formula supports adequate growth in cow's milk allergic infants. | children with cow's milk allergy (cma) are at risk for inadequate nutritional intake and growth. dietary management of cma, therefore, requires diets that are not only hypoallergenic but also support adequate growth in this population. this study assessed growth of cma infants when using a new amino acid-based formula (aaf) with prebiotics and probiotics (synbiotics) and evaluated its safety in the intended population. | 2015 | 25845680 |