| expression and characterization of a bifidobacterium adolescentis beta-mannanase carrying mannan-binding and cell association motifs. | the gene encoding β-mannanase (ec 3.2.1.78) baman26a from the bacterium bifidobacterium adolescentis (living in the human gut) was cloned and the gene product characterized. the enzyme was found to be modular and to contain a putative signal peptide. it possesses a catalytic module of the glycoside hydrolase family 26, a predicted immunoglobulin-like module, and two putative carbohydrate-binding modules (cbms) of family 23. the enzyme is likely cell attached either by the sortase mechanism (lpxt ... | 2013 | 23064345 |
| exploration of the genomic diversity and core genome of the bifidobacterium adolescentis phylogenetic group by means of a polyphasic approach. | in the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., bifidobacterium adolescentis, bifidobacterium catenulatum, and bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16s rrna gene and 16s-23s internal transcribed spacer (its) sequences, pulsed-field gel electrophoresis (pfge), and comparative genomic hybridization (cgh) assays. | 2013 | 23064340 |
| antiviral activity of bifidobacterium adolescentis spm 0214 against herpes simplex virus type 1. | herpes simplex virus type 1 (hsv-1) antibodies are found in up to 90 percent of the general population. about 30% of patients who have been exposed to hsv-1 develop recurrent infections, and this degree is continually increasing. in addition, resistance to all major anti-herpetic drugs such as acyclovir (acv) has been increasingly reported. these observations underscore the importance of discovering new therapeutic tools for the treatment of hsv-1 infections. bifidobacterium spp. has been studie ... | 2012 | 23054724 |
| evaluation of positive interaction for cell growth between bifidobacterium adolescentis and propionibacterium freudenreichii using a co-cultivation system with two microfiltration modules. | using a co-cultivation system developed previously, positive interaction for cell growth between bifidobacterium adolescentis and propionibacterium freudenreichii was evaluated. the total dry cell weight (dcw) of these two strains obtained in the co-cultivation system was 1.5-1.7-fold of the sum of the dcws obtained in two single cultivations of each bacterium. | 2013 | 23040994 |
| a novel sucrose phosphorylase from the metagenomes of sucrose-rich environment: isolation and characterization. | sucrose phosphorylase, an important enzyme mainly involved in the generic starch and sucrose pathways, has now caught the attention of researchers due to its transglycosylation activity. a novel sucrose phosphorylase, unspase, has been isolated, and its transglycosylation properties were characterized. compared with bisp, the sucrose phosphorylase from bifidobacterium adolescentis, unspase had two deleted regions in its c: -terminal. these deleted regions were probably equivalent to the importan ... | 2012 | 22806728 |
| antioxidative properties and inhibitory effect of bifidobacterium adolescentis on melanogenesis. | melanin is a dark pigment produced by melanocytes. tyrosinase is a key enzyme which catalyzes the rate-limiting step of melanogenesis. however, accumulation of melanin leads to various skin hyperpigmentation disorders. to find a novel skin-whitening agent, the antioxidant capacity of bifidobacterium adolescentis culture filtrate and inhibitory effect on melanogenesis were investigated. the antioxidant effects of b. adolescentis culture filtrate include 2,2-diphenyl-1-picryl-hydrazyl (dpph) radic ... | 2012 | 22806726 |
| antiviral activity of bifidobacterium adolescentis spm1005-a on human papillomavirus type 16. | probiotic lactic acid bacteria (lab) support a functional and balanced immune system, and contribute to immune modulatory effects in combatting microbial pathogens, including viruses. most cervical cancers are associated with anogenital region infection with high-risk (hr) human papillomavirus (hpv). in this study, we analyzed the antiviral activity of bifidobacterium adolescentis spm1005-a in the siha cervical cancer cell line expressing hpv type 16. | 2012 | 22788922 |
| a targeted gene knockout method using a newly constructed temperature-sensitive plasmid mediated homologous recombination in bifidobacterium longum. | bifidobacteria are the main component of the human microflora. we constructed a temperature-sensitive (ts) plasmid by random mutagenesis of the bifidobacterium-escherichia coli shuttle vector pkkt427 using error-prone pcr. mutant plasmids were introduced into bifidobacterium longum 105-a and, after screening approximately 3,000 colonies, candidate clones that grew at 30 °c but not at 42 °c were selected. according to dna sequence analysis of the ts plasmid, five silent and one missense mutations ... | 2012 | 22639142 |
| changes in growth and survival of bifidobacterium by coculture with propionibacterium in soy milk, cow's milk, and modified mrs medium. | bifidobacterium adolescentis int57 (int57) and propionibacterium freudenreichii subsp. shermanii atcc 13673 (atcc 13673) were grown either in coculture or as pure cultures in different media, such as cow's milk, soybean milk, and modified mrs medium. the viable cell counts of bacteria, changes in ph, concentrations of organic acids, and contents of various sugars were analyzed during incubation up to 7days. in soy milk, the survival of cocultured int57 was six times higher than the monocultured ... | 2012 | 22564451 |
| cloning and characterization of ginsenoside ra1-hydrolyzing beta-d-xylosidase from bifidobacterium breve k-110. | beta-d-xylosidase (e.c. 3.2.1.37) from bifidobacterium breve k-110, which hydrolyzes ginsenoside ra1 to ginsenoside rb2, was cloned and expressed in escherichia coli. the (his6)-tagged recombinant enzyme, designated as xlybk- 110, was efficiently purified using ni²⁺-affinity chromatography (109.9-fold, 84% yield). the molecular mass of xylbk- 100 was found to be 55.7 kda by sds-page. its sequence revealed a 1,347 bp open reading frame (orf) encoding a protein containing 448 amino acids, which sh ... | 2012 | 22534302 |
| quantification of human fecal bifidobacterium species by use of quantitative real-time pcr analysis targeting the groel gene. | quantitative real-time pcr assays targeting the groel gene for the specific enumeration of 12 human fecal bifidobacterium species were developed. the housekeeping gene groel (hsp60 in eukaryotes) was used as a discriminative marker for the differentiation of bifidobacterium adolescentis, b. angulatum, b. animalis, b. bifidum, b. breve, b. catenulatum, b. dentium, b. gallicum, b. longum, b. pseudocatenulatum, b. pseudolongum, and b. thermophilum. the bifidobacterial chromosome contains a single c ... | 2012 | 22307308 |
| distinct gut microbiota in southeastern african and northern european infants. | the intestinal microbiota composition in infants reflects the early environment. our objective was to compare the gut microbiota in 6-month-old infants living in rural malawi with children of the same age living in urban finland, both being breast-fed and having an age-appropriate diet typical for each area. | 2012 | 22228076 |
| selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid. | bifidobacterial strains are excessively sensitive to acidic conditions and this can affect their living ability in the stomach and fermented foods, and as a result, restrict their use as live probiotic cultures. the aim of the present study was to obtain bifidobacterial isolates with augmented tolerance to simulated gastrointestinal condition using cross-protection method. | 2010 | 22615595 |