| ecotoxicological effect characterisation of widely used organic uv filters. | chemical uv filters are used in sun protection and personal care products in order to protect consumers from skin cancer induced by ultraviolet (uv) radiation. the present study aims to evaluate the effects of three common uv filters butyl-methoxydibenzoylmethane (b-mdm) ethylhexyl-methoxycinnamate (ehmc) and octocrylene (ocr) on aquatic organism, focussing particularly on infaunal and epibentic invertebrates (chironomus riparius, lumbriculus variegatus, melanoides tuberculata and potamopyrgus a ... | 2012 | 22325435 |
| intrinsically green iron oxide nanoparticles? from synthesis via (eco-)toxicology to scenario modelling. | iron oxide nanoparticles (ionp) are currently being studied as green magnet resonance imaging (mri) contrast agents. they are also used in huge quantities for environmental remediation and water treatment purposes, although very little is known on the consequences of such applications for organisms and ecosystems. in order to address these questions, we synthesised polyvinylpyrrolidone-coated ionp, characterised the particle dispersion in various media and investigated the consequences of an ion ... | 2013 | 23255050 |
| fungal-bacterial consortia increase diuron degradation in water-unsaturated systems. | bioremediation of pesticide-polluted soil may be more efficient using mixed fungal-bacterial cultures rather than the individual strains alone. this may be due to cooperative catabolism, where the first organism transforms the pollutant to products which are then used by the second organism. in addition, fungal hyphae may function as transport vectors for bacteria, thereby facilitating a more effective spreading of degrader organisms in the soil. a more rapid mineralization of the phenylurea her ... | 2014 | 23973535 |
| arthrobacter nitrophenolicus sp. nov. a new 2-chloro-4-nitrophenol degrading bacterium isolated from contaminated soil. | strain sjcon(t), a 2-chloro-4-nitrophenol (2c4np) degrading bacterium, was isolated from soil collected from a pesticide-contaminated site in punjab, india. the strain, which stained gram positive, displayed a rod-coccus life cycle, and possessed a type a3α peptidoglycan (l-lys-l-ala3), mk-9(h2) as the major menaquinone, anteiso-c15 and iso-c15:0 as the major cellular fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and a glycolipid as the major polar lipids, s ... | 2013 | 28324343 |
| synergistic effect of calcium stearate and photo treatment on the rate of biodegradation of low density polyethylene spent saline vials. | the biodegradation of spent saline bottles, a low density polyethylene product (ldpe) by two selected arthrobacter sp. under in vitro conditions is reported. chemical and uv pretreatment play a vital role in enhancing the rate of biodegradation. treated ldpe film exhibits a higher weight loss and density when compared to untreated films. arthrobacter oxydans and arthrobacter globiformis grew better in medium containing pretreated film than in medium containing untreated film. the decrease in den ... | 2012 | 22822530 |
| beta-blockers in the environment: part ii. ecotoxicity study. | the increasing consumption of beta-blockers (bb) has caused their presence in the environment to become more noticeable. even though bb are safe for human and veterinary usage, ecosystems may be exposed to these substances. in this study, three selected bb: propranolol, metoprolol and nadolol were subjected to ecotoxicity study. ecotoxicity evaluation was based on a flexible ecotoxicological test battery including organisms, representing different trophic levels and complexity: marine bacteria ( ... | 2014 | 24975494 |
| surfactant-enhanced bioremediation of ddts and pahs in contaminated farmland soil. | field-scale bioremediation of dichlorodiphenyl trichloroethanes (ddts) and polycyclic aromatic hydrocarbons (pahs) contaminated farmland soil from the shenyang north new area of china was studied using the bacteria arthrobacter globiformis. the additive effects of different concentrations of biosurfactant rhamnolipids (rls) and anionic-nonionic mixed surfactant (sdbs-tween 80) were evaluated. ddt and pah removal rates by a. globiformis after 150 days of remediation were 52.1% and 21.9%, respecti ... | 2017 | 28562189 |
| an integrated approach with new strategies for qsar models and lead optimization. | computational drug design approaches are important for shortening the time and reducing the cost for drug discovery and development. among these methods, molecular docking and quantitative structure activity relationship (qsar) play key roles for lead discovery and optimization. here, we propose an integrated approach with core strategies to identify the protein-ligand hot spots for qsar models and lead optimization. these core strategies are: 1) to generate both residue-based and atom-based int ... | 2017 | 28361681 |
| genetic engineering of the biosynthesis of glycinebetaine leads to alleviate salt-induced potassium efflux and enhances salt tolerance in tomato plants. | tomato (solanum lycopersicum cv. 'moneymaker') was transformed with the choline oxidase gene coda from arthrobacter globiformis, which was modified to allow for targeting to both chloroplasts and the cytosol. glycine betaine (gb) was accumulated in transformed plants, while no detectable gb was found in wild-type (wt) plants. compared to wt plants, transgenic lines showed significantly higher photosynthetic rates (pn) and antioxidant enzyme activities and lower reactive oxygen species (ros) accu ... | 2017 | 28224920 |
| purification and characterization of hyaluronate lyase from arthrobacter globiformis a152. | a hyaluronate lyase was obtained by cultivating arthrobacter globiformis strain a152. the enzyme was purified to homogeneity from the supernatant by ammonium sulfate fractionation, q sepharose fast flow, and sephadex g-100 chromatography. the purification resulted in a 32.78-fold increase in hyaluronate lyase activity with specific activity of 297.2 u/mg. the molecular weight of the enzyme determined by sds-page was approximately 73.7 kda. using hyaluronic acid (ha) as a substrate, the maximal r ... | 2017 | 27878509 |
| purification and characterization of d-allulose 3-epimerase derived from arthrobacter globiformis m30, a gras microorganism. | an enzyme that catalyzes c-3 epimerization between d-fructose and d-allulose was found in arthrobacter globiformis strain m30. arthrobacter species have long been used in the food industry and are well-known for their high degree of safety. the enzyme was purified by ion exchange and hydrophobic interaction chromatographies and characterized as a d-allulose 3-epimerase (d-ae). the molecular weight of the purified enzyme was estimated to be 128 kda with four identical subunits. the enzyme showed ... | 2017 | 27713017 |
| role of f357 as an oxygen gate in the oxidative half-reaction of choline oxidase. | choline oxidase from arthrobacter globiformis catalyzes the oxidation of choline to glycine betaine by using oxygen as an electron acceptor. a partially rate limiting isomerization of the reduced wild-type enzyme during the reaction with oxygen was previously detected using solvent viscosity effects. in this study, we hypothesized that the side chains of m62 and f357, located at the entrance to the active site of choline oxidase, may be related to the slow isomerization detected. we engineered a ... | 2016 | 26907558 |
| exploiting orientation-selective deer: determining molecular structure in systems containing cu(ii) centres. | orientation-selective deer (double electron-electron resonance) measurements were conducted on a series of rigid and flexible molecules containing cu(ii) ions. a system with two rigidly held cu(ii) ions was afforded by the protein homo-dimer of copper amine oxidase from arthrobacter globiformis. this system provided experimental deer data between two cu(ii) ions with a well-defined distance and relative orientation to assess the accuracy of the methodology. evaluation of orientation-selective de ... | 2016 | 26837391 |
| increased iron-stress resilience of maize through inoculation of siderophore-producing arthrobacter globiformis from mine. | iron deficiency is common among graminaceous crops. ecologically successful wild grasses from iron-limiting habitats are likely to harbour bacteria which secrete efficient high-affinity iron-chelating molecules (siderophores) to solubilize and mobilize iron. such siderophore-producing rhizobacteria may increase the iron-stress resilience of graminaceous crops. considering this, 51 rhizobacterial isolates of dichanthium annulatum from iron-limiting abandoned mine (∼84% biologically unavailable ir ... | 2016 | 26632776 |
| crystal structure and mutational analysis of isomalto-dextranase, a member of glycoside hydrolase family 27. | arthrobacter globiformis t6 isomalto-dextranase (agimd) is an enzyme that liberates isomaltose from the non-reducing end of a polymer of glucose, dextran. agimd is classified as a member of the glycoside hydrolase family (gh) 27, which comprises mainly α-galactosidases and α-n-acetylgalactosaminidases, whereas agimd does not show α-galactosidase or α-n-acetylgalactosaminidase activities. here, we determined the crystal structure of agimd. agimd consists of the following three domains: a, c, and ... | 2015 | 26330557 |
| probing the molecular mechanisms in copper amine oxidases by generating heterodimers. | for some homodimeric copper amine oxidases (cuao), there is suggestive evidence of differential activity at the two active sites implying potential cooperativity between the two monomers. to examine this phenomenon for the arthrobacter globiformis cuao (agao), we purified a heterodimeric form of the enzyme for comparison with the homodimer. the heterodimer comprises an active wild-type monomer and an inactive monomer in which an active-site tyrosine is mutated to phenylalanine (y382f). this muta ... | 2015 | 25607656 |
| 2-keto-d-gluconate-yielding membrane-bound d-glucose dehydrogenase from arthrobacter globiformis c224: purification and characterization. | glucose dehydrogenase (glcdh) is the rate-limiting catalyst for microbial conversion of glucose to the important organic acid 2-ketogluconic acid (2kglca). in this study, a d-glucose dehydrogenase was purified from the industrial 2kglca producer arthrobacter globiformis c224. after four purification steps, the glcdh was successfully purified over 180 folds and specific activity of 88.1 u/mg. a single protein band of 87 kda was detected by sds-page. the purified glcdh had the broad substrate spec ... | 2015 | 25580683 |
| a multi-year assessment of the environmental impact of transgenic eucalyptus trees harboring a bacterial choline oxidase gene on biomass, precinct vegetation and the microbial community. | a 4-year field trial for the salt tolerant eucalyptus globulus labill. harboring the choline oxidase (coda) gene derived from the halobacterium arthrobacter globiformis was conducted to assess the impact of transgenic versus non-transgenic trees on biomass production, the adjacent soil microbial communities and vegetation by monitoring growth parameters, seasonal changes in soil microbes and the allelopathic activity of leaves. three independently-derived lines of transgenic e. globulus were com ... | 2014 | 24927812 |
| quality assessment of digested sludges produced by advanced stabilization processes. | the european union (eu) project routes aimed to discover new routes in sludge stabilization treatments leading to high-quality digested sludge, suitable for land application. in order to investigate the impact of different enhanced sludge stabilization processes such as (a) thermophilic digestion integrated with thermal hydrolysis pretreatment (tt), (b) sonication before mesophilic/thermophilic digestion (umt), and (c) sequential anaerobic/aerobic digestion (aa) on digested sludge quality, a bro ... | 2015 | 24903249 |
| toxicity screening of soils from different mine areas--a contribution to track the sensitivity and variability of arthrobacter globiformis assay. | this study used the arthrobacter globiformis solid-contact test for assessing the quality of soils collected in areas subjected to past and present mine activities in europe (uranium mine, portugal) and north africa (phosphogypsum pile, tunisia; iron mine, morocco). as to discriminate the influence of soils natural variability from the effect of contaminants, toxicity thresholds were derived for this test, based on the dataset of each study area. furthermore, the test sensitivity and variability ... | 2014 | 24797906 |
| structure of choline oxidase in complex with the reaction product glycine betaine. | choline oxidase from arthrobacter globiformis, which is involved in the biosynthesis of glycine betaine from choline, has been extensively characterized in its mechanistic and structural properties. despite the knowledge gained on the enzyme, the details of substrate access to the active site are not fully understood. the `loop-and-lid' mechanism described for the glucose-methanol-choline enzyme superfamily has not been confirmed for choline oxidase. instead, a hydrophobic cluster on the solvent ... | 2014 | 24531474 |
| a miniaturized solid contact test with arthrobacter globiformis for the assessment of the environmental impact of silver nanoparticles. | silver nanoparticles (agnps) are widely applied for their antibacterial activity. their increasing use in consumer products implies that they will find their way into the environment via wastewater-treatment plants. the aim of the present study was to compare the ecotoxicological impact of 2 differently designed agnps using the solid contact test for the bacterial strain arthrobacter globiformis. in addition, a miniaturized version of this test system was established, which requires only small-s ... | 2014 | 24477989 |
| carotene production from agro-industrial wastes by arthrobacter globiformis in shake-flask culture. | industrial waste substrates, sugarcane molasses, and corn steep liquor were used for production of carotenes by arthrobacter globiformis in this study. at the first stage, a one-factor-at-a-time approach was used for optimization of different media components such as carbon, nitrogen, mgso(4) · 7h(2)o, and kh(2)po(4), as well as ph, temperature, liquid medium volume, and inoculums level. the response surface method was further applied to determination of optimum values of process variables for m ... | 2014 | 24320236 |
| high-resolution crystal structure of copper amine oxidase from arthrobacter globiformis: assignment of bound diatomic molecules as o2. | the crystal structure of a copper amine oxidase from arthrobacter globiformis was determined at 1.08 å resolution with the use of low-molecular-weight polyethylene glycol (lmw peg; average molecular weight ∼200) as a cryoprotectant. the final crystallographic r factor and rfree were 13.0 and 15.0%, respectively. several molecules of lmw peg were found to occupy cavities in the protein interior, including the active site, which resulted in a marked reduction in the overall b factor and consequent ... | 2013 | 24311589 |
| deer-stitch: combining three- and four-pulse deer measurements for high sensitivity, deadtime free data. | over approximately the last 15 years the electron paramagnetic resonance (epr) technique of double electron electron resonance (deer) has attracted considerable attention since it allows for the precise measurement of the dipole-dipole coupling between radicals and thus can lead to distance information between pairs of radicals separated by up to ca. 8 nm. the "deadtime free" 4-pulse deer sequence is widely used but can suffer from poor sensitivity if the electron spin-echo decays too quickly to ... | 2012 | 22975240 |
| chromogenic bacteria related to bacterium globiforme. | | 1948 | 18909087 |
| chromogenic bacteria related to bacterium globiforme. | | 1948 | 16561494 |