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effect of ampicillin on the kinetics of colonization of streptococcus pneumoniae and lactobacillus fermentum in the respiratory tract of mice.ampicillin was selected to further study the effect of this antibiotic on the colonization capability of s. pneumoniae and l. fermentum intranasally inoculated in a mice experimental model. the sensitivity of s. pneumoniae and l. fermentum to antibiotics was evaluated by different "in vitro" techniques. the results showed that both microorganisms have a typical pattern of sensitivity to antibiotics in these assays. the "in vivo" experiments showed that the treatment with ampicillin increased the ...200415509298
diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food.traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ...200516104351
crystallization and preliminary x-ray crystallographic studies of glutamate racemase from lactobacillus fermenti.glutamate racemase catalyzes the conversion of l-glutamic acid to d-glutamic acid and vice versa. since d-glutamic acid is one of the essential amino acids present in peptidoglycan, glutamate racemase has been considered to be an attractive target for the design of new antibacterial drugs. glutamate racemase from lactobacillus fermenti has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 8000 as a precipitant. the crystals belong to the orthorhombic space g ...200516510993
isolation and characterization of a lactobacillus fermentum temperate bacteriophage from chinese yogurt.the aim of this study was to investigate the properties of temperate bacteriophage of lactobacillus fermentum, based on its morphology, restriction patterns, protein profile and the impact on the growth of host strain.200616968297
small surface-associated factors mediate adhesion of a food-isolated strain of lactobacillus fermentum to caco-2 cells.in a search for bacteria having putative probiotic activity, we screened a collection of food-isolated microorganisms for the ability to survive at low ph in the presence of bile salts and for the production of antimicrobial compounds active against a number of animal pathogens. among these, we found a strain that we classified as a member of lactobacillus fermentum sp., and we further investigated its features. this organism was able to adhere to human enterocyte-like (caco-2) cells with high e ...200516125909
the mechanism of action of alpha-amylase from lactobacillus fermentum on maltooligosaccharides.the action pattern of lactobacillus fermentum alpha-amylase (fermenta) was examined using a series of maltooligosaccharides (g2-g7) as substrates. structurally, this enzyme has a molecular mass (106 kda) almost twofold higher than alpha-amylases from mammalians and cereals. the product pattern was investigated through an analysis of products and substrates using hpaec with pulsed amperometric detection. fermenta was consistent with an endo-type of amylase. the bond cleavage frequencies were stud ...200616531129
quantitative real-time pcr analysis of fecal lactobacillus species in infants receiving a prebiotic infant formula.the developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. generally, bifidobacterium and lactobacillus predominate the microbiota of breast-fed infants. in intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. however, the lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in gre ...200616597930
expression of the immunity protein of plantaricin 423, produced by lactobacillus plantarum 423, and analysis of the plasmid encoding the bacteriocin.plantaricin 423 is a class iia bacteriocin produced by lactobacillus plantarum isolated from sorghum beer. it has been previously determined that plantaricin 423 is encoded by a plasmid designated ppla4, which is now completely sequenced. the plantaricin 423 operon shares high sequence similarity with the operons of coagulin, pediocin pa-1, and pediocin ach, with small differences in the dna sequence encoding the mature bacteriocin peptide and the immunity protein. apart from the bacteriocin ope ...200617056693
calcium modulates interactions between bacteria and hydroxyapatite.bacterial adhesion onto hydroxyapatite is known to depend on the surface properties of both the biomaterial and the bacterial strain, but less is known about the influence of the composition of the aqueous medium. here, the adhesion of streptococcus mutans and 3 different lactobacilli on powdered hydroxyapatite was shown to change with ca2+ concentration. the effect depends on the surface properties of each strain. adhesion of lactobacillus fermentum and salivarius (and of streptococcus mutans a ...200617122166
electrode system for the determination of microbial populations.determinations of microbial populations were carried out by using a new electrode system composed of two electrodes. each electrode was constructed from a platinum anode and a silver peroxide cathode. the anode of the reference electrode was covered with cellulose dialysis membrane. the response time of the electrode system was 15 min in culture broth, and current differences between the two electrodes were proportional to populations of microbial cells in cultures of saccharomyces cerevisiae an ...197916345334
lactobacillus fermentum br11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (dss) in rats.current treatments for inflammatory bowel disease (ibd) are relatively ineffective. recently, probiotics have emerged as a potential treatment modality for numerous gastrointestinal disorders, including ibd. few probiotics, however, have undergone appropriate preclinical screening in vivo. the current study compared the effects of four candidate probiotics on development of dextran sulfate sodium (dss)-induced colitis in rats. sprague dawley rats were gavaged 1 ml of the potential probiotic (1 x ...200717150273
application of potential probiotic lactobacillus fermentum ad1 strain in healthy dogs.probiotic utilization is becoming increasingly popular in veterinary medicine. however, only few probiotic products are available commercially for use in dogs in our market. therefore, the aim of our study was to determine the properties of new potential probiotic lactobacillus fermentum ad1 strain-own canine isolate and to investigate its effect on several microbiological and biochemical parameters in healthy dogs. the strain expressed in vitro survival by ph 3.0 after 3h (86.8%) and in the pre ...200616701618
quantitative evaluation of adhesion of lactobacilli isolated from human intestinal tissues to human colonic mucin using surface plasmon resonance (biacore assay).to isolate lactobacilli from the mucus layer of the human intestine and evaluate their adhesion abilities using a biacore assay.200717184326
immunoenhancing effects of a new probiotic strain, lactobacillus fermentum pl9005.the immunoenhancing effects of lactobacillus fermentum pl9005 were assessed via mouse intragastric inoculation. the number of immunoglobulin a-positive cells in the small intestine, cd4+ t lymphocytes in the peripheral blood, and the lymphocyte proliferation response to mitogen stimulation (lipopolysaccharide) increased in mice fed l. fermentum pl9005. the lactic acid concentration also increased dose dependently in the small intestine of mice fed l. fermentum pl9005. no differences were found i ...200515771184
lactic acid bacteria and yeasts associated with spontaneous fermentations during the production of sour cassava starch in brazil.sour cassava starch is a traditional fermented food used in the preparation of fried foods and baked goods such as traditional cheese breads in brazil. thirty samples of sour cassava starch were collected from two factories in the state of minas gerais. the samples were examined for the presence of lactic acid bacteria, yeasts, mesophilic microorganisms, bacillus cereus and faecal coliforms. lactic acid bacteria and yeasts isolates were identified by biochemical tests, and the identities were co ...200516153731
characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation.attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ...200616213052
resistance to antimicrobial agents in lactobacilli isolated from caper fermentations.a collection of lactobacilli comprising species of lactobacillus plantarum (43 isolates), lactobacillus brevis (9 isolates) and lactobacillus fermentum (6 isolates) obtained from spontaneous fermentations of capers (the fruits of capparis spinosa) were investigated for resistance to antimicrobial agents. all isolates were resistant to vancomycin and teicoplanin (mic > 16 microg/ml). resistance to ciprofloxacin (mic > 2 microg/ml) was detected in all isolates of l. brevis and l. fermentum as well ...200516284934
lactobacillus bacteremia, species identification, and antimicrobial susceptibility of 85 blood isolates.data regarding antimicrobial susceptibility of clinical lactobacillus isolates are scarce, and appropriate interpretation criteria for susceptibility tests are not available.200616447101
cellular fatty acid profiles of lactobacillus and lactococcus strains in relation to the oleic acid content of the cultivation medium.cellular fatty acids of 10 strains of lactic acid bacteria were analyzed. the purpose of this work was to find lactic acid bacteria with high lactobacillic acid contents. the bacteria studied were unable to synthesize oleic acid. some strains did not synthesize lactobacillic acid, although all were able to form dihydrosterculic acid. twenty-one to thirty-four percent of the fatty acid content of lactobacillus fermentum and l. buchneri was lactobacillic acid, and these species were chosen for fut ...199516535200
growth of lactic acid bacteria and rhizopus oligosporus during barley tempeh fermentation.the zygomycete rhizopus oligosporus is traditionally used to ferment soybean tempeh, but it is also possible to ferment other legumes and cereals to tempeh. the traditional soybean tempeh harbours a multitude of microorganisms with potentially beneficial or detrimental effects on quality. lactic acid bacteria (lab) have positive effects on the safety of soybean tempeh, but the effects of lab on r. oligosporus growth have not been investigated. we have developed a cereal grain tempeh by fermentin ...200515979185
dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in ghana.the ghanaian cocoa bean heap fermentation process was studied through a multiphasic approach, encompassing both microbiological and metabolite target analyses. a culture-dependent (plating and incubation, followed by repetitive-sequence-based pcr analyses of picked-up colonies) and culture-independent (denaturing gradient gel electrophoresis [dgge] of 16s rrna gene amplicons, pcr-dgge) approach revealed a limited biodiversity and targeted population dynamics of both lactic acid bacteria (lab) an ...200717277227
caries-related plaque microcosm biofilms developed in microplates.in vivo dental plaque biofilms consist of complex communities of oral bacteria that are a challenge to replicate in vitro. the aim of this investigation was to establish human dental plaque microcosms in microplates to reflect conditions that are relevant to dental caries. microcosm plaque biofilms were initiated from the saliva of two different donors, grown for up to 10 days in 24-welled microplates on thermanox coverslips in various types of artificial saliva with and without sucrose, which w ...200717311629
molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates.the objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains.200717311634
identification of glucose-fermenting bacteria present in an in vitro model of the human intestine by rna-stable isotope probing.16s rrna-based stable isotope probing (sip) and nuclear magnetic resonance (nmr) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating the human intestine. the tim-2 in vitro model of the human intestine was inoculated with a gi tract microbiota resembling that of the small intestine, to which subsequently 4, 20 or 40 mm of [u-(13)c]-glucose were added. rna was extracted from lumen samples after 0 (control), 1, 2 and 4 h and subjecte ...200717313661
bacterial population in traditional sourdough evaluated by molecular methods.to study the microbial communities in artisanal sourdoughs, manufactured by traditional procedure in different areas of sicily, and to evaluate the lactic acid bacteria (lab) population by classical and culture-independent approaches.200516033455
functional and structural characterization of thermostable d-amino acid aminotransferases from geobacillus spp.d-amino acid aminotransferases (d-aats) from geobacillus toebii sk1 and geobacillus sp. strain kls1 were cloned and characterized from a genetic, catalytic, and structural aspect. although the enzymes were highly thermostable, their catalytic capability was approximately one-third of that of highly active bacilli enzymes, with respective turnover rates of 47 and 55 s(-1) at 50 degrees c. the geobacillus enzymes were unique and shared limited sequence identities of below 45% with d-aats from meso ...200616461714
development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, lactobacillus thermotolerans, in chicken feces.a new real-time pcr assay was successfully developed using a taqman fluorescence probe for specific detection and enumeration of a novel bacterium, lactobacillus thermotolerans, in chicken feces. the specific primers and probe were designed based on the l. thermotolerans 16s rrna gene sequences, and these sequences were compared to those of all available 16s rrna genes in the genbank database. the assay, targeting 16s rrna gene, was evaluated using dna from a pure culture of l. thermotolerans, d ...200516085805
surgical site abscess caused by lactobacillus fermentum identified by 16s ribosomal rna gene sequencing.we report the first case of surgical site abscess caused by lactobacillus fermentum from a 53-year-old woman with squamous cell carcinoma of the esophagus after transthoracic esophagectomy and neoadjuvant chemoirradiation. 16s rrna gene sequencing is a useful tool to better characterize the epidemiology and clinical significance of l. fermentum.200717368805
species diversity and relative abundance of vaginal lactic acid bacteria from women in uganda and korea.lactobacilli play an important role in maintaining vaginal health of women. the aim of this study was to compare the species richness and relative abundance of lactobacillus and other lactic acid bacteria in women of two geographically distant countries, uganda and korea.200717381754
microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods.fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ...200516332762
reclassification of lactobacillus cellobiosus rogosa et al. 1953 as a later synonym of lactobacillus fermentum beijerinck 1901.the name lactobacillus cellobiosus is validly published, but the species is often neglected in taxonomic studies, due to its high similarity to lactobacillus fermentum. in the present paper, literature data concerning the two species were reviewed. phylogenetic placement of l. cellobiosus was obtained based on 16s rdna sequences, and genetic similarity was further investigated by comparing partial reca gene sequences for the type strains of l. cellobiosus and l. fermentum. based on the high iden ...200415143028
effects of commensal bacteria on intestinal morphology and expression of proinflammatory cytokines in the gnotobiotic pig.a germ-free neonatal pig model was established to determine the effects of bacterial colonization by different species on small intestinal morphology and proinflammatory cytokine gene expression. two experimental groups of 16 pigs were aseptically delivered by cesarian section and allocated into 4 gnotobiotic isolators. pigs were either maintained germ-free (gf), or were orally inoculated with either a single strain of nonpathogenic escherichia coli (ec) or lactobacillus fermentum (lf) or conven ...200616946402
alteration of the canine small-intestinal lactic acid bacterium microbiota by feeding of potential probiotics.five potentially probiotic canine fecal lactic acid bacterium (lab) strains, lactobacillus fermentum lab8, lactobacillus salivarius lab9, weissella confusa lab10, lactobacillus rhamnosus lab11, and lactobacillus mucosae lab12, were fed to five permanently fistulated beagles for 7 days. the survival of the strains and their potential effects on the indigenous intestinal lab microbiota were monitored for 17 days. denaturing gradient gel electrophoresis (dgge) demonstrated that the five fed lab str ...200617021203
a thermostable alpha-galactosidase from lactobacillus fermentum crl722: genetic characterization and main properties.alpha-galactosidase (alpha-gal) enzyme, which is encoded by the mela gene hydrolyzes alpha-1,6 galactoside linkages found in sugars, such as raffinose and stachyose. these alpha-galacto-oligosaccharides (alpha-gos), which are found in large quantities in vegetables, such as soy, can cause gastrointestinal disorders in sensitive individuals because monogastric animals (including humans) do not posses alpha-gal in the gut. the use of microbial alpha-gal is a promising alternative to eliminate alph ...200617048069
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...200717049799
effect of lactobacillus fermentum on serum lipids in subjects with elevated serum cholesterol.there is increasing interest in the use of natural therapies to reduce elevated low density lipoprotein (ldl) cholesterol. this study assessed the effects of pcc lactobacillus fermentum on ldl cholesterol and other lipid fractions.200617126768
the microbiology of ghanaian cocoa fermentations analysed using culture-dependent and culture-independent methods.export of cocoa beans is of great economic importance in ghana and several other tropical countries. raw cocoa has an astringent unpleasant taste and a spontaneous fermentation is the first step in a process leading to cocoa beans with the characteristic cocoa flavour and taste. the microbiology of ghanaian cocoa fermentations was investigated using culture-dependent and culture-independent methods. samples were collected at 12 hour intervals during 96-144 hour tray and traditional heap fermenta ...200717161485
rapid removal of lead and cadmium from water by specific lactic acid bacteria.cadmium and lead are highly toxic metals. people are exposed to them primarily through food and water. available conventional methods (precipitation, flocculation, ion exchange, and membrane filtration) for removal of these metals from water at low concentrations are claimed to be expensive and inefficient. different microbes have been proposed to be an efficient and economical alternative in heavy metal removal from water. in this work, specific lactic acid bacteria (lab) were assessed for thei ...200717184867
characterisation and biochemical properties of predominant lactic acid bacteria from fermenting cassava for selection as starter cultures.a total of 375 lactic acid bacteria were isolated from fermenting cassava in south africa, benin, kenya and germany, and were characterised by phenotypic and genotypic tests. these could be divided into five main groups comprising strains of facultatively heterofermentative rods, obligately heterofermentative rods, heterofermentative cocci, homofermentative cocci and obligately homofermentative rods, in decreasing order of predominance. most of the facultatively heterofermentative rods were iden ...200717188771
characterization of genes involved in fructose utilization by lactobacillus fermentum.the genes encoding phosphoglucose isomerase (frui) and fructokinase (fruk) of lactobacillus fermentum nrrl-b-1932 were sequenced. they constituted an operon, which is involved in fructose metabolism of this strain by channeling intracellular fructose into the phosphoketolase pathway. a third open reading frame, unkr, upstream of the operon was identified as homologous to genes of laci/galr family repressors. the unkr repressor's role in transcriptional control of the fruik operon could, however, ...200616741753
importance of the host specificity in the selection of probiotic bacteria.the gastrointestinal tract is a complex and dynamic ecosystem. commensal microorganisms (c), which proliferate in the intestine from birth, are crucial for gut homeostasis while non commensal (nc) microorganisms are transient and enter the organism from the environment and foods. we studied comparatively the influence of oral administration of c and nc lactobacillus fermentum and lactobacilus acidophilus on the gut-associated lymphoid tissue (galt) of conventional mice. to determine the importan ...200616836803
lactobacillus fermentum attenuates the proinflammatory effect of yersinia enterocolitica on human epithelial cells.lactobacilli represent a major component of the human microbiota. in this study we investigated whether and how lactobacillus fermentum inhibits the proinflammatory responses of human epithelial cells on yersinia enterocolitica infection.200717206643
a comparative study of the preventative effects exerted by two probiotics, lactobacillus reuteri and lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis.the intestinal anti-inflammatory effects of two probiotics isolated from breast milk, lactobacillus reuteri and l. fermentum, were evaluated and compared in the trinitrobenzenesulfonic acid (tnbs) model of rat colitis. colitis was induced in rats by intracolonic administration of 10 mg tnbs dissolved in 50% ethanol (0.25 ml). either l. reuteri or l. fermentum was daily administered orally (5 x 10(8) colony-forming units suspended in 0.5 ml skimmed milk) to each group of rats (n 10) for 3 weeks, ...200717217564
nonstarter lactobacillus strains as adjunct cultures for cheese making: in vitro characterization and performance in two model cheeses.nonstarter lactic acid bacteria are the main uncontrolled factor in today's industrial cheese making and may be the cause of quality inconsistencies and defects in cheeses. in this context, adjunct cultures of selected lactobacilli from nonstarter lactic acid bacteria origin appear as the best alternative to indirectly control cheese biota. the objective of the present work was to study the technological properties of lactobacillus strains isolated from cheese by in vitro and in situ assays. mil ...200717881674
the biodiversity of predominant lactic acid bacteria in dolo and pito wort for the production of sorghum beer.to quantify and identify the predominant lactic acid bacteria (lab) in dolo and pito wort processing, and to examine their biodiversity at strain level.200717897178
probiotic effects on 5-fluorouracil-induced mucositis assessed by the sucrose breath test in rats.the sucrose breath test (sbt) was employed to noninvasively assess the efficacy of probiotics in 5-fluorouracil (5-fu)-induced intestinal mucositis. dark agouti rats were allocated to 5 groups (n = 10): 5-fu + l. fermentum br 11, 5-fu + l. rhamnosus gg, 5-fu + b. lactis bb 12, 5-fu + skim milk (sm), and saline + sm. probiotics were administered by oral gavage for 10 days. mucositis was induced on day 7 by intraperitoneal injection of 5-fu (150 mg/kg) or vehicle (saline). rats were sacrificed 72 ...200717237997
two lactobacillus strains, isolated from breast milk, differently modulate the immune response.the ability of two different lactobacillus strains (lactobacillus salivarius cect5713 and lactobacillus fermentum cect5716), isolated from human breast milk, to modulate the immune response was examined.200717241338
characteristics of the adhesion of pcc lactobacillus fermentum vri 003 to peyer's patches.the characteristics of the adhesion of pcc lactobacillus fermentum vri 003 to peyer's patches was studied in vitro. the adhesion of l. fermentum 003 was strongly inhibited in the presence of d-mannose and methyl-alpha-d-mannoside although other carbohydrates tested, such as n-acetyl-glucosamine, d-galactose, d-glucose and l-fucose, did not affect the adhesion. lactobacillus fermentum 003 was shown to strongly attach to mannose immobilized on a surface using bsa, suggesting that l. fermentum 003 ...200616842353
characterization of lactobacilli isolated from caper berry fermentations.to determine the metabolic and functional properties of lactobacilli isolated from caper fermentation.200717241365
modeling of urea degradation in white and rosé wines by acid urease.the specific activity of a whole cell acid urease preparation was tested in five wines manufactured in the apulia region of italy in the 2003 vintage at both short and long treatment times, thus confirming the validity of the pseudo-first-order kinetic model to describe urea removal in real wines. the ratio between the experimental pseudo-first-order kinetic rate constant (kie) for any real wine tested and that (ki) referred to a model wine solution having the same composition and ph reduced fro ...200717328559
molecular identification of vaginal lactobacilli isolated from bulgarian women.lactobacilli play an important role in maintaining the vaginal health of women. the development of suitable bacterial replacement therapies for the treatment of vaginosis requires knowledge of the vaginal lactobacilli species representation. the aim of this study was to identify at the species level vaginal lactobacillus isolates obtained from bulgarian women in childbearing age by using different molecular methods. twenty-two strains of lactobacilli isolated from vaginal samples were identified ...200616871423
lactobacillus fermentum aca-dc 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (tnbs)-induced colitis and salmonella infection in murine models.lactobacillus fermentum aca-dc 179, lactobacillus plantarum aca-dc 287 and streptococcus macedonicus aca-dc 198 were studied for their probiotic potential. firstly, strains were screened for antimicrobial activity towards a broad range of target strains, including lactic acid bacteria, food spoilage and pathogenic bacteria. l. fermentum aca-dc 179 was active against five streptococci, including the two pathogenic strains streptococcus oralis lmg 14532t and streptococcus pneumoniae lmg 14545t. s. ...200818077037
antibiotic susceptibility profiles of lactobacillus reuteri and lactobacillus fermentum.lactobacillus reuteri and lactobacillus fermentum, which are commonly used as food processing aids and probiotics, can potentially act as reservoirs of antibiotic resistance genes. acquired resistance genes may be transferred via the food chain or in the gastrointestinal tract to pathogenic bacteria. knowledge of the distributions of antibiotic mics for a species is needed when using a phenotypic method to assess the presence of acquired resistance genes. in the present study, 56 l. reuteri and ...200717340877
oral intake of lactobacillus fermentum cect5716 enhances the effects of influenza vaccination.we studied the coadjuvant capability of oral consumption of the breast-milk-isolated strain lactobacillus fermentum (cect5716) for an anti-influenza vaccine.200717352961
induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion.some strains of lactobacilli can stimulate macrophages and dendritic cells to secrete il-12, which plays a key role in activating innate immunity. we examined the il-12-inducing ability of 47 lactobacillus strains belonging to 10 species in mouse peritoneal macrophages, and characterized the properties important for the induction of il-12. although considerable differences in il-12-inducing ability were observed among the strains tested, almost all strains belonging to the lactobacillus casei gr ...200616899663
effect of proton pump inhibitor pantoprazole on growth and morphology of oral lactobacillus strains.proton pump inhibitors (ppi) used to suppress acid secretion in the stomach are among the most widely prescribed medications. there is emerging evidence of proton secretion elsewhere in the aerodigestive tract, and acidic microenvironments are integral to oral flora such as lactobacillus. the hypothesis of this study is that the growth rate and morphology of oral lactobacillus strains are effected by ppis.200818176340
disinfectant test against monoculture and mixed-culture biofilms composed of technological, spoilage and pathogenic bacteria: bactericidal effect of essential oil and hydrosol of satureja thymbra and comparison with standard acid-base sanitizers.to assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline chemical disinfectants.200818217930
assessment of urea degradation rate in model wine solutions by acid urease from lactobacillus fermentum.the specific activity (pia) of a whole cell acid urease preparation was assessed in model wine solutions at different levels of malic (m) and lactic acids, metabisulfite, ethanol, and ph by performing a central composite design. m and then ph were found to be the most controlling variables, their effects being practically coincident but of opposite sign. for urea concentrations up to approximately 1 mol m(-3) the ammonium formation rate was assumed of the pseudo-first-order with respect to urea, ...200616910712
effects of bacterial colonization on the porcine intestinal proteome.the gastrointestinal tract harbors a complex community of bacteria, of which many may be beneficial. studies of germ-free animal models have shown that the gastrointestinal microbiota not only assists in making nutrients available for the host but also contributes to intestinal health and development. we studied small intestinal protein expression patterns in gnotobiotic pigs maintained germ-free, or monoassociated with either lactobacillus fermentum or non-pathogenic escherichia coli. a common ...200717542629
different probiotic properties for lactobacillus fermentum strains isolated from swine and poultry.systematic procedures were used to evaluate the probiotic properties of lactobacillus fermentum (l. fermentum) strains isolated from swine and poultry. the major properties included their capabilities to adhere to the intestinal epithelium of swine and poultry, the inhibition on pathogenic bacteria, and their tolerance to the gastric juice and bile salts. results showed that l. fermentum strains from poultry digestive tract showed better adherence to the swine intestine and chicken crop epitheli ...200717544731
population dynamics and metabolite target analysis of lactic acid bacteria during laboratory fermentations of wheat and spelt sourdoughs.four laboratory sourdough fermentations, initiated with wheat or spelt flour and without the addition of a starter culture, were prepared over a period of 10 days with daily back-slopping. samples taken at all refreshment steps were used for determination of the present microbiota. furthermore, an extensive metabolite target analysis of more than 100 different compounds was performed through a combination of various chromatographic methods including liquid chromatography-mass spectrometry and ga ...200717557853
effect of ethanol rinse, lactobacillus fermentum inoculation, and modified atmosphere on ground chicken meat quality.ground chicken breast meat was prepared using combinations of the following treatments: ethanol rinse before grinding, inoculation with lactobacillus fermentum after grinding, and modified atmosphere packaging in either 90% o(2) and 10% co(2) or 90% n(2) and 10% co(2). control treatments included water rinse and noninoculation with l. fermentum. packaged meat was refrigerated and sampled for various shelf-life quality indices on d 0, 3, and 6. the inoculation with l. fermentum had little or no e ...200717575191
a probiotic, lactobacillus fermentum me-3, has antioxidative capacity in soft cheese spreads with different fats.our aim was to develop a prototype of a functional spread cheese containing both a specific probiotic and n-3 fatty acids and to analyze the viability of the probiotic and stability of n-3 fatty acids during 4 wk of shelf life. lactobacillus fermentum me-3 (lf me-3) isolated from a healthy estonian child has been shown to have probiotic and antioxidative properties in several recent studies. in the current study this promising bacterial strain was combined with vegetable oils rich in nutritional ...200717582099
survival rate analysis of freeze-dried lactic acid bacteria using the arrhenius and z-value models.the survival rate of five freeze-dried bacteria species, lactobacillus plantarum, lactobacillus pentosus, weisella paramesenteroides, leuconostoc mesenteroides, and lactobacillus fermentum, was described in terms of reaction rate constants (d or k) and temperature sensitivity of rate constants (z or ea). the freeze-dried strains were stored under vacuum at 55, 37, and 4 degrees c for 168 h, 17 days, and 2 months, respectively. d-values decreased and k increased with an increase of the storage te ...200818326201
probiotics for the treatment of women with bacterial vaginosis.this review considers whether probiotics are effective agents for the treatment and/or prevention of bacterial vaginosis (bv). there seems to be an association between the absence of, or low concentrations of, vaginal lactobacilli and the development of bv. many studies have suggested that the presence of h2o2-producing vaginal lactobacilli may protect against bv, although some studies do not support this hypothesis. in-vitro studies have suggested that certain specific strains of lactobacilli a ...200717633390
preparation and in vitro analysis of microencapsulated live lactobacillus fermentum 11976 for augmentation of feruloyl esterase in the gastrointestinal tract.fa (ferulic acid) is a well-known phenolic phytochemical present in plant cell walls. various studies have indicated that fa has many physiological functions in the prevention of chronic disease. it has been shown to play an important chemoprotective role in degenerative diseases. fa also shows strong antioxidant and nitrite-scavenging potential and anticarcinogenic and antiinflammatory properties. the in vivo physiological importance of fa depends on its availability for absorption. dietary fib ...200817640174
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.200717650194
commensal bacteria and expression of two major intestinal chemokines, teck/ccl25 and mec/ccl28, and their receptors.ccl25/teck and ccl28/mec are cc chemokines primarily expressed in thymic dendritic cells and mucosal epithelial cells. their receptors, ccr9 and ccr10, are mainly expressed on t and b lymphocytes. in human, mouse, pig and sheep ccl25 and ccl28 play an important role in the segregation and the compartmentalization of the mucosal immune system. as evidenced by early comparisons of germ-free and conventional animals, the intestinal bacterial microflora has a marked effect on host intestinal immune ...200717653288
[effects of a lactic acid bacteria community sfc-2 treated on rice straw].aimed to utilize rice straw and lessen the pressure of environment, the rice straw was used as the fermentation material, and a lactic acid bacteria community sfc-2 from my laboratory was inoculated into the rice straw to investigate the inoculation effects. after 30 days fermentation, the inoculated fermented straw smelt acid-fragrant, and the ph value was 3.8, which was lower than the control of 4.1. furthermore, lactic acid concentration was more than that in the control. especially l-lactic ...200717674756
enterocyte proliferation and apoptosis in the caudal small intestine is influenced by the composition of colonizing commensal bacteria in the neonatal gnotobiotic pig.we previously reported marked differences in small intestinal morphology, including changes in crypt depth and villous height, after inoculation of germ-free pigs with different bacterial species. in an attempt to identify the mechanisms governing changes in villous morphology associated with bacterial colonization, 2 gnotobiotic experiments were performed. in each experiment, 16 piglets were allocated to 4 treatment groups including germ-free (gf), monoassociation with lactobacillus fermentum ( ...200717785595
image analysis for monitoring the barley tempeh fermentation process.to develop a fast, accurate, objective and nondestructive method for monitoring barley tempeh fermentation.200717897216
lactobacillus fermentum ess-1 with unique growth inhibition of vulvo-vaginal candidiasis pathogens.the aim of this study was to characterize human isolates of lactobacillus species for their capacity to interfere with the growth of different strains of candida species in vitro in the search for a potential probiotic. growth inhibition of candida species was screened using an agar-overlay method. inhibiting strains were selected to assay the effect of a cell-free lactobacillus culture filtrate (lcf) on the growth of isolates of candida albicans and candida glabrata. a total of 126 human lactob ...200717965352
influence of turning and environmental contamination on the dynamics of populations of lactic acid and acetic acid bacteria involved in spontaneous cocoa bean heap fermentation in ghana.the influence of turning and environmental contamination on six spontaneous cocoa bean heap fermentations performed in ghana was studied through a multiphasic approach, encompassing both microbiological (culture-dependent and culture-independent techniques) and metabolite target analyses. a sensory analysis of chocolate made from the fermented, dried beans was performed as well. only four clusters were found among the isolates of acetic acid bacteria (aab) identified: acetobacter pasteurianus, a ...200817993565
rapid identification of the three major species of dairy obligate heterofermenters lactobacillus brevis, lactobacillus fermentum and lactobacillus parabuchneri by species-specific duplex pcr.in this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (ohl) by classical microbiological tests, as well as 23 ohl-type strains, was investigated by pcr-based methods and gene sequencing. using these techniques, 51% of the cheese isolates were actually identified as ohl. the non-ohl isolates were identified to the leuconostoc, lactobacillus, weisella, pediococcus or strept ...200818510560
2-de and ms analysis of interactions between lactobacillus fermentum i5007 and intestinal epithelial cells.lactobacillus is a probiotic commonly used for supplementation to human and animal diets. in this study, we used 2-de and ms to analyze changes in the proteomes of lactobacillus and intestinal epithelial cells in two model systems. the in vivo and in vitro models were involved the inoculation of lactobacillus fermentum i5007 into the rabbit jejunum for 4 h and the culture of the bacterium with caco-2 cells for 1 h, respectively. our results indicate that, after exposure to the intestinal environ ...200718004711
diversity of lactic acid bacteria population in ripened parmigiano reggiano cheese.the diversity of dominant lactic acid bacteria population in 12 months ripened parmigiano reggiano cheeses was investigated by a polyphasic approach including culture-dependent and independent methods. traditional plating, isolation of lab and identification by 16s rdna analysis showed that strains belonging to lactobacillus casei group were the most frequently isolated. lactobacillus helveticus, lactobacillus delbrueckii subsp. lactis, lactobacillus parabuchneri, and lactobacillus buchneri spec ...200818524408
microflora of feta cheese from four greek manufacturers.the components of the microflora of four feta cheeses, produced by different greek manufacturers, were determined by culture dependent and independent techniques. isolates from microbiological media were first grouped by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) and then representatives of each dgge group were sequenced for identification purposes. dna and rna, extracted directly from the cheese, were subjected to pcr-dgge. moreover, feta cheeses were subjected ...200818555549
grana padano cheese whey starters: microbial composition and strain distribution.the aim of this work was to evaluate the species composition and the genotypic strain heterogeneity of dominant lactic acid bacteria (lab) isolated from whey starter cultures used to manufacture grana padano cheese. twenty-four grana padano cheese whey starters collected from dairies located over a wide geographic production area in the north of italy were analyzed. total thermophilic lab streptococci and lactobacilli were quantified by agar plate counting. population structure of the dominant a ...200818620769
bacteriocin-producing lactobacillus strains isolated from poto poto, a congolese fermented maize product, and genetic fingerprinting of their plantaricin operons.thirty one bacteriocin-producing lactobacillus isolates were identified among 135 lactobacilli isolated from the congolese fermented maize product poto poto, during the preparation and from the finished product. using species-specific pcr and 16s rrna gene sequencing, 28 and 3 isolates were identified as l. plantarum and l. fermentum, respectively. cluster analysis of rapd-pcr fingerprints revealed two main groups (g1 and g2) plus the l. fermentum isolate c4-13. group g1 contained 23 isolates wi ...200818620772
factors affecting the adsorption of bacteriocins to lactobacillus sakei and enterococcus sp.bacteriocins bacjw3bz and bacjw6bz produced by lactobacillus plantarum, and bacjw11bz and bacjw15bz produced by lactobacillus fermentum, inhibit gram-positive and gram-negative bacteria. treatment of enterococcus sp. hklhs and lactobacillus sakei dsm 20017 with these bacteriocins deformed the cells and resulted in dna and beta-galactosidase leakage. the bacteriocins adsorbed to sensitive and resistant strains. optimal adsorption of bacjw3bz and bacjw6bz to enterococcus sp. hklhs was recorded at ...200718025582
technological properties of lactobacillus fermentum involved in the processing of dolo and pito, west african sorghum beers, for the selection of starter cultures.technological properties of lactobacillus fermentum isolates involved in spontaneous fermentation of dolo and pito wort were examined to select starter cultures.200818031523
the adhesion of putative probiotic lactobacilli to cultured epithelial cells and porcine intestinal mucus.to investigate the adhesion of lactobacilli and their subsequent competitive exclusion ability against pathogens.200818081774
a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius.samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ...200818774196
expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.nucleoside 2'-deoxyribosyltransferase (ndt) is used to synthesize unnatural 2'-deoxyribonucleosides, modified mostly on the heterocyclic base. here we describe a strategy for improving 2,3-dideoxyribosyl(ddr) transfer activity of ndt by combining mutagenesis and in vivo selection in e. coli.200818776470
growth-promoting effects of single-dose intragastrically administered probiotics in chickens.1. live body weight gain (wg) and food conversion efficiency (fce) were measured over a 4-week period in chicks (female, kabir strain), inoculated intragastrically at one week of age with either a single dose (1 ml) of 10(7) live bacterial cells of lactobacillus fermentum (lbf) or a strain of lactobacillus spp. (lb), named autruche 4 (a4), suspended in 1 ml of phosphate-buffered saline (pbs, ph 7.0); controls (n = 10) were inoculated only with pbs. the same commercial chicken feed was used throu ...200718085457
purification and partial characterization of novel bacteriocin l23 produced by lactobacillus fermentum l23.lactobacillus fermentum strain l23 produced a small bacteriocin, designated bacteriocin l23, with an estimated molecular mass of < 7000 da. isolation, purification, and partial characterization of bacteriocin l23 are described. it displayed a wide inhibitory spectrum including both gram-negative and gram-positive pathogenic strains and two species of candida. the antibacterial activity of cell-free culture supernatant fluid was not affected by catalase or urease but was abolished by the proteoly ...200818172715
identification of lactic acid bacteria from fermented tea leaves (miang) in thailand and proposals of lactobacillus thailandensis sp. nov., lactobacillus camelliae sp. nov., and pediococcus siamensis sp. nov.eighteen rod-shaped homofermentatives, six heterofermentatives, and a coccal homofermentative lactic acid bacteria were isolated from fermented tea leaves (miang) produced in the northern part of thailand. the isolates were placed in a monophyletic cluster consisting of lactobacillus and pediococcus species. they were divided into seven groups by phenotypic and chemotaxonomic characteristics, dna-dna similarity, and 16s rrna gene sequences. groups i to vi belonged to lactobacillus and group vii ...200717429157
prebiotic and synbiotic fructooligosaccharide administration fails to reduce the severity of experimental colitis in rats.opposing effects of the prebiotic, fructooligosaccharide, have been reported in experimental colitis. we compared the effects of the prebiotic, fructooligosaccharide, alone and in synbiotic combination with lactobacillus fermentum br11, on the development of dextran sulfate sodium-induced colitis in rats. rats consumed an 18 percent casein-based diet or diet supplemented with 6 percent fructooligosaccharide or maltodextrin for 14 days. the synbiotic group was gavaged 1 ml of l. fermentum br11 (1 ...200717429712
microencapsulated bacterial cells can be used to produce the enzyme feruloyl esterase: preparation and in-vitro analysis.biotechnological production of ferulic acid, a precursor of vanillin, is an attractive alternative for various industries due to the high price and demand for natural ferulic acid. feruloyl esterase has been identified as a key enzyme involved in microbial transformations of ferulic acid to vanillin. several fungal feruloyl esterases have been purified and characterized for their use in the production of ferulic acid. this paper, for the first time, discusses the use of lactic acid bacteria for ...200717483939
bactericidal activity of culture fluid components of lactobacillus fermentum strain 90 ts-4 (21) clone 3, and their capacity to modulate adhesion of candida albicans yeast-like fungi to vaginal epithelial cells.antagonistic activities of l. fermentum strain 90 ts-4 (21), l. casei atcc 27216, and l. acidophilus atcc 4356 and bactericidal activity of lactobacillus culture fluid towards e. coli strain k12, s. aureus, and s. epidermidis test cultures were studied. the bactericidal effect of l. fermentum strain 90 ts-4 (21) clone 3 culture fluid preparation (ph 6.0) on the test cultures was dose-dependent. adhesion of c. albicans yeast-like fungi to vaginal epitheliocytes was more pronounced for strains iso ...200718225764
ability of lactobacillus fermentum to overcome host alpha-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya alpha-galacto-oligosaccharides.soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of alpha-galacto-oligosaccharides. these are not digested in the small intestine due to the natural absence of tissular alpha-galactosidase in mammals. the passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. the aim of the work reported here was to assess the ability of alpha-gala ...200818230145
rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using pcr-based methods.in this study, pcr-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. consensus primers were used in conventional and reverse transcription pcr to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. all enterococci, staphylococcus epidermidis, lactobacillus brevis, lactobacillus curvatus, and lactobacillus fermentum strains and 1 of ...200818236668
[d-lactic acidosis in a child with short bowel syndrome].d-lactic acidosis is a rare and severe complication of short bowel syndrome in children that may result from important ileal bacterial overgrowth by lactobacilli. intestinal flora (lactobacilli) is responsible for the production of d-lactic acid after fermentation of food carbohydrates.200818242965
oral administration of the probiotic lactobacillus fermentum vri-003 and mucosal immunity in endurance athletes.to evaluate the ability of a probiotic lactobacillus fermentum vri-003 (pcc) to enhance the mucosal immune system of elite athletes.201018272539
probiotics and blueberry attenuate the severity of dextran sulfate sodium (dss)-induced colitis.we studied the anti-inflammatory properties of probiotic strains and blueberry in a colitis model. the disease activity index (dai) was significantly lower on days 9 and 10 in all groups compared to the colitis control. myeloperoxidase (mpo) and bacterial translocation to the liver and to the mesenteric lymph nodes (mln) decreased significantly in all groups compared to colitis control. cecal enterobacteriaceae count decreased significantly in blueberry with and without probiotics compared to th ...200818274903
diversity and dynamics of lactobacilli populations during ripening of rdo camembert cheese.the diversity and dynamics of lactobacillus populations in traditional raw milk camembert cheese were monitored throughout the manufacturing process in 3 dairies. culture-dependent analysis was carried out on isolates grown on acidified de man - rogosa - sharpe agar and lactobacillus anaerobic de man rogosa sharpe agar supplemented with vancomycin and bromocresol green media. the isolates were identified by polymerase chain reaction - temperature gradient gel electrophoresis (pcr-tgge) and (or) ...200818388993
characterization of endogenous plasmids from lactobacillus salivarius ucc118.the genome of lactobacillus salivarius ucc118 comprises a 1.83-mb chromosome, a 242-kb megaplasmid (pmp118), and two smaller plasmids of 20 kb (psf118-20) and 44 kb (psf118-44). annotation and bioinformatic analyses suggest that both of the smaller plasmids replicate by a theta replication mechanism. furthermore, it appears that they are transmissible, although neither possesses a complete set of conjugation genes. plasmid psf118-20 encodes a toxin-antitoxin system composed of pemi and pemk homo ...200818390685
lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses.two strains of lactic acid bacteria were isolated from faeces of two actively racing thoroughbred horses. the isolates formed a subcluster in the lactobacillus reuteri phylogenetic group, closely related to lactobacillus fermentum, l. gastricus, l. ingluviei and l. mucosae, by phylogenetic analysis based on 16s rrna gene sequences. levels of dna-dna relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. biochemical and ...200818398194
live probiotic bifidobacterium lactis bacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell culture.wheat gliadin induces severe intestinal symptoms and small-bowel mucosal damage in coeliac disease patients. at present, the only effective treatment for the disease is a strict life-long gluten-free diet. in this study we investigated whether probiotics lactobacillus fermentum or bifidobacterium lactis can inhibit the toxic effects of gliadin in intestinal cell culture conditions. the ability of live probiotics to inhibit peptic-tryptic digested gliadin-induced damage to human colon cells caco- ...200818422736
metabolism of prebiotic products containing beta(2-1) fructan mixtures by two lactobacillus strains.the capacity of two probiotic strains, isolated from human breast milk, to use several beta(2-1) fructan mixtures as carbon and energy source in in vitro cultures has been tested. results showed that both strains, lactobacillus gasseri cect5714 and lactobacillus fermentum cect5716, reached higher growth levels on culture media containing fructooligosaccharide mixtures produced by enzymatic synthesis, compared to those obtained by inulin hydrolysis. furthermore, the shortest beta(2-1) fructan, ke ...200818434219
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