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effects of probiotic lactobacillus paracasei-enriched artichokes on constipated patients: a pilot study.to determine whether the consumption of artichokes enriched with a probiotic lactobacillus paracasei strain affects fecal microbiota composition, fecal enzyme activity, and short-chain fatty acids production and symptom profile in patients suffering from constipation.201020495470
probiotic lactobacillus paracasei effect on cariogenic bacterial flora.lactobacillus paracasei has been demonstrated to inhibit the growth of many pathogenic microbes such as streptococcus mutans, in vitro. however, its clinical application remains unclear. here, we examined whether a novel probiotic l. paracasei gmnl-33 may reduce the caries-associated salivary microbial counts in healthy adults. seventy-eight subjects (aged 20 to 26) had completed this double-blinded, randomized, placebo-controlled study. a probiotic/test (n = 42) and a control group (n = 36) too ...201020502929
enzymatic activity of lactic acid bacteria (with antimicrobial properties) isolated from a traditional spanish cheese.twenty-four strains of lactic acid bacteria (lab) isolated from a traditional spanish cheese (genestoso cheese) were evaluated for their enzymatic activities (acidifying and proteolytic abilities and carboxypeptidase, aminopeptidase, dipeptidase, caseinolytic and esterase activities), in order to select indigenous strains of technical interest for the manufacture of cheese. these strains were selected on the basis of their antimicrobial activity relative to five reference strains and were identi ...201020510776
caries inhibition by and safety of lactobacillus paracasei dsmz16671.lactobacillus paracasei dsmz16671, even if heat-killed, sensitively co-aggregates mutans streptococci specifically. mutans streptococci are strongly implicated in caries induction in humans and rodents. we hypothesized: (1) that s. mutans recoveries from rats' teeth in vivo will decrease, with an associated decrease in caries, if these lactobacilli are fed to rats in an established caries model; and (2) that toxicological assays of these lactobacilli will show them to be benign. four groups of s ...201020519491
impact of heat-inactivated lactobacillus casei and lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis.heat-inactivated lactobacillus casei lock 0900, l. casei lock 0908 and lactobacillus paracasei lock 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic t(h)1 cytokines (interleukin-12, interleukin-18, interferon-gamma, tumor necrosis factor-alpha) and regulatory transforming growth factor-beta(1)), but did not stimulate pro-allergic interleukin-5. the lactobacilli-mixture remarkably enhanced the t(h)1 response compared to ...201020526842
protective effects of lactobacillus paracasei f19 in a rat model of oxidative and metabolic hepatic injury.the liver is susceptible to such oxidative and metabolic stresses as ischemia-reperfusion (i/r) and fatty acid accumulation. probiotics are viable microorganisms that restore the gut microbiota and exert a beneficial effect on the liver by inhibiting bacterial enzymes, stimulating immunity, and protecting intestinal permeability. we evaluated lactobacillus paracasei f19 (lp-f19), for its potential protective effect, in an experimental model of i/r (30 min ischemia and 60 min reperfusion) in rats ...201020576921
inhibition of shigella sonnei adherence to ht-29 cells by lactobacilli from chinese fermented food and preliminary characterization of s-layer protein involvement.in this study, seven lactobacilli with a high degree of antagonistic activity against three pathogens and good adherence to ht-29 cells were selected. the ability of these seven lactobacilli to inhibit adhesion of shigella sonnei to intestinal mucosa was studied on cultured ht-29 cells. lactobacilli were added simultaneously with, before or after s. sonnei to test for their effectiveness in exclusion, competition and displacement assays, respectively. lactobacillus paracasei subp. paracasei m5-l ...201020600857
clinical trial on the efficacy of a new symbiotic formulation, flortec, in patients with acute diarrhea: a multicenter, randomized study in primary care.few randomized studies have been carried out on adult patients affected by acute diarrhea especially in primary care, which is the natural context for this kind of disorder. lactobacillus paracasei b 21060 is a novel strain of lactobacillus, which has been shown to be effective in relieving symptoms associated with diarrhea of irritable bowel syndrome subtype and in shortening diarrhea duration. in this study, we compared the therapeutic efficacy, safety, and tolerability of a new symbiotic form ...201020616746
improving the stability of probiotic bacteria in model fruit juices using vitamins and antioxidants.this study examined the survival of probiotic bacteria in a model fruit juice system. three different strains of probiotic bacteria were used in this study: howaru lactobacillus rhamnosus hn001, howaru bifidobacterium lactis hn001, and lactobacillus paracasei lpc 37. the probiotic bacteria were inoculated into model juice with various vitamins and antioxidants, namely white grape seed extract, green tea extract, vitamin b2, vitamin b3, vitamin b6, vitamin c, and vitamin e. the model juice withou ...201020629884
specific metabolic activity of ripening bacteria quantified by real-time reverse transcription pcr throughout emmental cheese manufacture.bacterial communities of fermented foods are usually investigated by culture-dependent methods. real-time quantitative pcr (qpcr) and reverse transcription (rt)-qpcr offer new possibilities to quantify the populations present and their metabolic activity. the aim of this work was to develop qpcr and rt-qpcr methods to assess the metabolic activity and the stress level of the two species used as ripening cultures in emmental cheese manufacture, propionibacterium freudenreichii and lactobacillus p ...201020630608
screening and selection of stress resistant lactobacillus spp. isolated from the marine oyster (crassostrea gigas).we attempted to isolate lactobacillus spp. from the marine oyster (crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. a total of 83 lactobacilli strains were isolated from oyster. they were all gram-positive, rod-shaped and catalase-negative. by performing a stress resistance assay, we selected eighteen isolates. based on 16s rrna gene sequencing, lactobacillus paracasei was the most prevalent species among the selected isolates. ...201020633689
splenic abscess caused by lactobacillus paracasei.splenic abscess is relatively uncommon. infections caused by lactobacilli, which are usually one of the constituents of normal flora of human gut and vagina, are also very uncommon. here we report a case of splenic abscess caused by lactobacillus paracasei. we performed a literature review for this rare entity. immunocompromised status of the patient, who had uncontrolled diabetes, could have contributed to the pathogenesis of this rare disease.201120669039
enhancement of antimicrobial and antimutagenic activities of korean barberry (berberis koreana palib.) by the combined process of high-pressure extraction with probiotic fermentation.to evaluate the combined effects of high pressure extraction (hpe) and probiotic fermentation on the antimicrobial and antimutagenic activities, berberis koreana was subjected to 500 mpa for 30 min and then fermented with bifidobacterium longum b6 (hpe-blf) and lactobacillus paracasei (hpe-lpf) at 37 °c for 6 days.201020672332
the dnak gene as a molecular marker for the classification and discrimination of the lactobacillus casei group.it is hard to accurately identify specific species of the lactobacillus casei group using phenotypic techniques alone. some strains of this species group are considered to be probiotic and are widely applied in the food industry. in this study, we compared the use of two phylogenetic markers, the 16s rrna and dnak genes, for species discrimination of the members of the l. casei group using sequencing and rflp. the results showed that l. casei, lactobacillus paracasei, lactobacillus zeae and lact ...201120700765
characterization of the cryptic plasmid ptxw from lactobacillus paracasei txw.a cryptic plasmid from lactobacillus paracasei txw isolated from koumiss, designated as ptxw, was sequenced and characterized. it is 3178 bp in length with a g+c content of 42.9%. the plasmid ptxw was predicted to encode 4 putative orfs. repb shared high homology with initiator proteins of plasmids from the rolling circle replication (rcr) pmv158 family. copg was predicted to encode a transcriptional repressor, which was involved in plasmid copy number control together with a putative antisense ...201120709099
randomised, double-blind and placebo-controlled study using new probiotic lactobacilli for strengthening the body immune defence against viral infections.the aim of this study was to investigate whether consumption of lactobacillus plantarum heal 9 (dsm 15312) and lactobacillus paracasei 8700:2 (dsm 13434) could affect naturally acquired common cold infections in healthy subjects.201120803023
dietary modulation of gut functional ecology studied by fecal metabonomics.a major source of intestinal metabolites results from both host and microbial processing of dietary nutrients. (1)h nmr-based metabolic profiling of mouse feces was carried out over time in different microbiome mouse models, including conventional (n = 9), conventionalized (n = 10), and "humanized" gnotobiotic mice inoculated with a model of human baby microbiota (hbm, n = 17). hbm mice were supplemented with lactobacillus paracasei with (n = 10) and without (n = 7) prebiotics. animals not suppl ...201020806900
resistance to acidic environments of caries-associated bacteria: bifidobacterium dentium and bifidobacterium longum.oral bifidobacteriaceae, bifidobacterium dentium and bifidobacterium longum, are known to be isolated together with mutans streptococci and lactobacilli from caries lesions, suggesting that these bifidobacteriaceae are caries associated and acid resistant. this study aimed to investigate effects of acidification on b. dentium and b. longum, and to compare them with those on streptococcus mutans, streptococcus sanguinis and lactobacillus paracasei. effects of acidification, growth ability in a co ...201020814202
comparison of aqueous plant extracts before and after fermentation with lactobacillus paracasei ls-2 on cytokine induction and antioxidant activity.this study was conducted to investigate the antioxidant properties and inhibitory effects on the inflammation-related cytokines of plant extracts fermented with lactobacillus paracasei ls-2 in comparison with the unfermented aqueous plant extract. attempted have also been made to identify fermented plant extracts that display no cytotoxicity against murine macrophage cells, while still maintaining their biological characteristics. most of the fermented plant extracts showed reduced cytotoxicity. ...201020839635
decreased fat storage by lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein (angptl4).intervention strategies for obesity are global issues that require immediate attention. one approach is to exploit the growing consensus that beneficial gut microbiota could be of use in intervention regimes. our objective was to determine the mechanism by which the probiotic bacteria lactobacillus paracasei ssp paracasei f19 (f19) could alter fat storage. angiopoietin-like 4 (angptl4) is a circulating lipoprotein lipase (lpl) inhibitor that controls triglyceride deposition into adipocytes and h ...201020927337
immunomodulating activity of lactobacillus paracasei subsp. paracasei ntu 101 in enterohemorrhagic escherichia coli o157h7-infected mice.the present study investigated the immunomodulating activity of lactobacillus paracasei subsp. paracasei ntu 101 in enterohemorrhagic escherichia coli o157:h7-infected balb/c mice. mice were given l. paracasei subsp. paracasei ntu 101 (10(8) colony-forming units) for 7 days, before and after the challenge with e. coli o157:h7. feeding lactobacillus for 7 days resulted in an increased postchallenge weight gain and lower cumulative morbidity rates. we observed the upregulation of dendritic cells, ...201020942489
probiotic properties of lactobacillus strains isolated from the feces of breast-fed infants and taiwanese pickled cabbage.this study assessed potential probiotic lactobacillus strains isolated from the feces of breast-fed infants and from taiwanese pickled cabbage for their possible use in probiotic fermented foods by evaluating their (i) in vitro adhesive ability, resistance to biotic stress, resistance to pathogenic bacteria, and production of β-galactosidase; (ii) milk technological properties; and (iii) in vivo adhesive ability, intestinal survival and microbial changes during and after treatment. five lactobac ...201020951815
lactobacillus paracasei cncm i-2116 (st11) inhibits substance p-induced skin inflammation and accelerates skin barrier function recovery in vitro.over the past few decades the number of people presenting reactive skin has increased in industrial countries. skin inflammation mediated by neuropeptides and impaired skin barrier function are both underlying features of reactive skin conditions. live microorganisms defined as probiotics have been successfully used to improve health status in humans. beyond the effects on intestinal microbiota, some probiotic strains display potent immune-modulatory properties at the skin level. the aim of this ...201020965806
co-culturing of lactobacillus paracasei subsp. paracasei with a lactobacillus delbrueckii subsp. delbrueckii mutant to make high cell density for increased lactate productivity from cassava bagasse hydrolysate.to increase the productivity of lactic acid, a co-culture of lactobacilli was made by mixing 1:1 ratio of lactobacillus paracasei subsp. paracasei and a fast growing l. delbrueckii subsp. delbrueckii mutant. the culture was embedded on to polyurethane foam (puf) cubes as a biofilm and used for fermentation. in order to prevent the cell leakage, the puf cubes were further entrapped in calcium cross-linked alginate. the maximum lactic acid production using a high cell density free culture was >38 ...201020972788
selection, application and monitoring of lactobacillus paracasei strains as adjunct cultures in the production of gouda-type cheeses.raw milk cheeses have more intense flavours than cheeses made from pasteurized milk and harbour strains with potential adjunct properties. two lactobacillus paracasei strains, r-40926 and r-40937, were selected as potential adjunct cultures from a total of 734 isolates from good quality artisan raw milk gouda-type cheeses on the basis of their prevalence in different cheese types and/or over several production batches, safety and technological parameters. conventional culturing, isolation and id ...201021036412
widely distributed lysogeny in probiotic lactobacilli represents a potentially high risk for the fermentative dairy industry.prophages account for most of the genetic diversity among strains of a given bacterial species, and represent a latent source for the generation of virulent phages. in this work, a set of 30 commercial, collection and dairy-isolated lactobacillus casei group strains were used. a species-specific pcr assay allowed a reclassification, mainly of strains previously considered lactobacillus casei, into either lactobacillus paracasei or lactobacillus rhamnosus. all the strains were induced with mitomy ...201021131090
the incidence of necrotizing enterocolitis is increased following probiotic administration to preterm pigs.preterm birth and necrotizing enterocolitis (nec) is associated with inappropriate gut colonization and immunity, which may be improved by probiotic bacteria. using a preterm pig model of nec, we investigated the effects of probiotics on intestinal structure, function, microbiology, and immunology in the immediate postnatal period. just after birth, caesarean-delivered preterm pigs were inoculated with lactobacillus paracasei, bifidobacteria animalis, and streptococcus thermophilus (total 2.4 × ...201021178092
recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice.we have previously demonstrated that oral administration of a metabolically active bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (cla), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 cla and omega-3 (n-3) fatty acids in liver and adipose tissue. in this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production ...201021178166
protective effect of lactofermented beetroot juice against aberrant crypt foci formation and genotoxicity of fecal water in rats.the aim of the study was to investigate the effects of beetroot juice fermented by lactobacillus brevis 0944 and lactobacillus paracasei 0920 (fbj) on carcinogen induction of aberrant crypt foci (acf) in rat colon. n-nitroso-n-methylurea (mnu) was used as carcinogen, which was administrated intragastrically at a dose of 50mg/kg on the 23rd and 26th day of the experiment. additionally, we investigated the cytotoxicity and genotoxicity of fecal water from experimental animals in the caco 2 cell li ...201021185162
distinctive anti-allergy properties of two probiotic bacterial strains in a mouse model of allergic poly-sensitization.we compared the immunomodulatory properties of bifidobacterium longum ncc 3001 and lactobacillus paracasei ncc 2461 in a mouse model of poly-sensitization to birch and grass pollen allergens. mucosal application of both strains at the time of sensitization and challenge led to significant suppression of airway inflammation and down-regulated allergen-specific immune responses. in contrast, in the mice treated with probiotics prior to sensitization and challenge, only b. longum displayed protecti ...201121216308
sake lees fermented with lactic acid bacteria prevents allergic rhinitis-like symptoms and ige-mediated basophil degranulation.we tested the effect of oral administration of fermented sake lees with lactic acid bacteria (feslab) on a murine model of allergic rhinitis upon immunization and nasal sensitization with ovalbumin (ova). we used lactobacillus paracasei npsrik-4 (isolated from sake lees), and l. brevis npsriv-8 (from fermented milk) as starter strains to produce the feslab. oral feslab administration resulted in the development of significantly fewer sneezing symptoms than those seen in sham control animals give ...201121228477
beneficial effects of a synbiotic supplement on self-perceived gastrointestinal well-being and immunoinflammatory status of healthy adults.the use of synbiotics as health promoters is still poorly defined, and human intervention studies are scarce. this study was designed to evaluate the effects of a commercialized synbiotic product containing lactobacillus acidophilus la5, bifidobacterium animalis ssp. lactis bb-12, lactobacillus delbrueckii ssp. bulgaricus, lactobacillus paracasei ssp. paracasei, streptococcus thermophilus, and fructooligosaccharides on the self-reported gastrointestinal well-being and the immunoinflammatory stat ...201121244240
integrative expression system for delivery of antibody fragments by lactobacilli.a series of expression cassettes which mediate secretion or surface display of antibody fragments was stably integrated in the chromosome of lactobacillus paracasei. l. paracasei producing surface-anchored variable domain of llama heavy chain (vhh) (arp1) directed against rotavirus showed efficient binding to rotavirus and protection in the mouse model of rotavirus infection.201121257814
beneficial effect of a diet containing heat-killed lactobacillus paracasei k71 on adult type atopic dermatitis.the purpose of this study was to investigate the clinical effect of a supplementary diet containing heat-killed lactic acid bacterium lactobacillus paracasei k71 (lab diet) on adult patients with atopic dermatitis (ad). a randomized, double-blind, placebo-controlled study was conducted in 34 adult type ad subjects who were treated with conventional topical corticosteroid and tacrolimus. lab diet or placebo was added over 12 weeks. the primary end-point was the clinical severity of ad which was e ...201021269308
a novel vector for lactic acid bacteria that uses a bile salt hydrolase gene as a potential food-grade selection marker.a novel vector pm4ab for lactic acid bacterial was developed using a bile salt hydrolase gene from lactobacillus plantarum as a potential food-grade selection marker. the 3.0-kb pm4ab consisted of the replicon of lactobacillus plasmid pm4, a multiple cloning site and the bsh gene, which was constructed by elimination of a 5.5-kb non-food-grade dna fragment from an 8.5-kb intermediate vector pbempm4ab. for electroporation into lactobacillus paracasei x9, a high transformation efficiency of 4.0±1. ...201121277345
conversion of squid pen by a novel strain lactobacillus paracasei subsp. paracasei tku010, and its application in antimicrobial and antioxidants activity.tku010 was isolated from infant vomited milk and identified as lactobacillus paracasei subsp. paracasei. tku010 had desirable properties concerning its ability to withstand adverse conditions in the gastrointestinal tract. the hydrolysate of casein enhanced the growth of tku010 most obviously (17.20-18.25 od(660)), followed by the hydrolysate of spp (16.00-15.06 od(660)). incubating with spp, both the culture supernatant of tku010 on the first day and the fourth day showed inhibitory activities ...201021282904
[genetic identification of antagonistically active lactobacillus strains isolated from the oral cavity of healthy individuals].examination of dental deposits from 45 healthy individuals detected 3 lactobacillus strains showing a high antagonism toward test cultures. the api 50 ch "bio merieux" test systems were employed to identify strains as lactobacillus fermentum 39, lactobacillus rhamnosus 24 and lactobacillus paracasei 50. the results of analyzing the sequences of the 16s rrna genes of the test strains confirmed this identification, except for the latter strain. the taxonomic status of the third strain l. rhamnosus ...201021313755
a 90-day oral toxicity study on a new strain of lactobacillus paracasei in rats.lactobacillus paracasei is a species of bacteria that has been suggested to have probiotic benefits. to investigate the subchronic toxicity of l. paracasei gw080, a 90-feeding study was conducted in rats. sprague-dawley rats were randomly divided into four groups (10 rats/sex/group) and treated with 0, 1.25, 2.5, and 5.0 g/kg body weight (approximately equivalent to 0, 2.5×10(9), 5.0×10(9) and 1×10(10)cfu/kg bw) of test material by gavage for 90 days. daily clinical observations and weekly measu ...201121335050
efficacy of lactobacillus paracasei sub. paracasei f19 on abdominal symptoms in patients with symptomatic uncomplicated diverticular disease: a pilot study.the standard therapeutic approach for symptomatic uncomplicated diverticular disease (dd) remains to be defined, and only a few studies have tested the efficacy of probiotics in these patients.201121372765
influence of a combination of two potential probiotic strains, lactobacillus rhamnosus imc 501-« and lactobacillus paracasei imc 502-« on bowel habits of healthy adults.this study aims to investigate the effect of different kinds of food products enriched with a combination of two potential probiotic strains, lactobacillus rhamnosus imc 501(-«) and lactobacillus paracasei imc 502(-«), on bowel habits of healthy adults.201121395626
effect of lactobacillus paracasei st11 on a nasal provocation test with grass pollen in allergic rhinitis.probiotics have been associated with prevention and improvement of symptoms in atopic diseases such as atopic dermatitis. however, few studies exist that document their efficacy for upper airways allergies such as allergic rhinitis.201121395878
effect of a probiotic intake on oxidant and antioxidant parameters in plasma of athletes during intense exercise training.the aim of this study was to evaluate the effect of lactobacillus rhamnosus imc 501 and lactobacillus paracasei imc 502 on oxidative stress in athletes during a four-week period of intense physical activity. two groups of twelve subjects each were selected for this analysis. the first group consumed a daily dose of a mixture of the two probiotic strains (1:1 l. rhamnosus imc 501 and l. paracasei imc 502; ~10(9) cells/day) for 4 weeks. the second group (control) did not consume any supplements du ...201121400082
application of the snapshot minisequencing assay to species identification in the lactobacillus casei group.this study used group-specific pcr combined with snapshot minisequencing for species identification within the lactobacillus casei group. the l. casei group-specific pcr primer pair was designed using the rpoa gene sequence. a snapshot minisequencing assay using dnak as a target gene was developed, and five snp primers were designed by analysing the conserved regions of the dnak sequences. the specificity of the minisequencing assay was evaluated using 63 strains of l. casei group species. the r ...201121440058
effects of probiotic fermented milk on symptoms and intestinal flora in patients with irritable bowel syndrome: a randomized, placebo-controlled trial.the effect of probiotics on ibs symptoms has been mixed, but remains an intriguing treatment option with appeal to the patient.201121443416
antihypertensive effects of lactobacillus-fermented milk orally administered to spontaneously hypertensive rats.products fermented with lactic acid bacteria may show antihypertensive effects via substances such as angiotensin i-converting enzyme inhibitor (acei) and ?-aminobutyric acid (gaba). it was previously found that milk fermented with lactobacillus paracasei subsp. paracasei ntu 101 (101fm) or lactobacillus plantarum ntu 102 (102fm) has acei and gaba activities. this study aimed to investigate the antihypertensive effects of 101fm and 102fm orally administered to spontaneously hypertensive rats (sh ...201121446645
role of the probiotic strain lactobacillus paracasei lmgp22043 carried by artichokes in influencing faecal bacteria and biochemical parameters in human subjects.to evaluate the positive influence of the probiotic strain lactobacillus paracasei lmgp22043 carried by artichokes into the human gut with special reference to faecal bacterial balance, short-chain fatty acid concentrations and enzyme activities in a randomized, double-blind human trial in comparison with probiotic-free artichokes (control).201121447019
functional milk beverage fortified with phenolic compounds extracted from olive vegetation water, and fermented with functional lactic acid bacteria.functional milk beverages (fmb100 and fmb200) fortified with phenolic compounds (100 and 200mg/l) extracted from olive vegetable water, and fermented with ?-amino butyric acid (gaba)-producing (lactobacillus plantarum c48) and autochthonous human gastro-intestinal (lactobacillus paracasei 15n) lactic acid bacteria were manufactured. a milk beverage (mb), without addition of phenolic compounds, was used as the control. except for a longer latency phase of fmb200, the three beverages showed an alm ...201121458095
antimicrobial activity against shigella sonnei and probiotic properties of wild lactobacilli from fermented food.four lactobacilli strains (lactobacillus paracasei subp. paracasei m5-l, lactobacillus rhamnosus j10-l, lactobacillus casei q8-l and l. rhamnosus gg (lgg), were systematically assessed for the production of antimicrobial substances active towards shigella sonnei, escherichia coli and salmonella typhimurium. agar-well assay showed that the four lactobacilli strains displayed strong antibacterial activity towards s. sonnei. the nature of antimicrobial substances was also investigated and shown to ...201121466951
evaluation of the functional potential of weissella and lactobacillus isolates obtained from nigerian traditional fermented foods and cow's intestine.the characterisation of 24 lactic acid bacteria (lab) isolates from nigerian traditional fermented dairy foods, including some cow's intestine isolates, was conducted in order to select isolates for potential use as probiotics. lab isolates were identified by partial sequencing the 16s rrna gene as belonging to the species lactobacillus paracasei, lactobacillus brevis and mainly weissella confusa. at the end of a characterisation process, 2 l. paracasei and 2 w. confusa isolates were selected, a ...201121482440
oral lactobacillus paracasei improves skin barrier function recovery and reduces local skin inflammation. 201121489918
lactobacillus saniviri sp. nov. and lactobacillus senioris sp. nov., isolated from human faeces.two gram-positive-staining strains, yit 12363t and yit 12364t, isolated from human faeces were rod-shaped, non-motile, asporogenous, facultative anaerobic and did not exhibit catalase activity. comparative analyses of 16s rrna, phes and rpoa gene sequence demonstrated that the novel strains were members of the genus lactobacillus. on the basis of 16s rrna gene sequence similarity, the type strains of lactobacillus casei (95.3%), lactobacillus paracasei subsp. paracasei (95.6%), lactobacillus par ...201121515698
survival of probiotic adjunct cultures in cheese and challenges in their enumeration using selective media.various selective media for enumerating probiotic and cheese cultures were screened, with 6 media then used to study survival of probiotic bacteria in full-fat and low-fat cheddar cheese. commercial strains of lactobacillus acidophilus, lactobacillus casei, lactobacillus paracasei, or bifidobacterium lactis were added as probiotic adjuncts. the selective media, designed to promote growth of certain lactic acid bacteria (lab) over others or to differentiate between lab, were used to detect indivi ...201121524512
genetic diversity of lactobacillus paracasei isolated from in situ human oral biofilms.aim:  to determine the genetic diversity and possible origin of lactobacillus paracasei found in the oral biofilm. methods and results:  lactobacilli were isolated from a biofilm model, formed in situ prior to and during a period of exposure to 20% sucrose solution (28 days), using rogosa agar. the lactobacillus colonies were randomly selected (n = 222) and subcultured. the isolates were identified using phes or rpoa gene sequence analysis. lactobacilli identified as lact. paracasei (n = 75) wer ...201121535332
potential application of the nisin z preparation of lactococcus lactis w8 in preservation of milk.aims:  the aim of the study is to evaluate the effectiveness of the preparation of nisin z from lactococcus lactis w8-fermented milk in controlling the growth of spoilage bacteria in pasteurized milk. methods and results:  spoilage bacteria isolated from pasteurized milk at 8 and 15°c were identified as enterococcus italicus, enterococcus mundtii, enterococcus faecalis, bacillus thuringiensis, bacillus cereus, lactobacillus paracasei, acinetobacter sp., pseudomonas fluorescens and enterobacter a ...201121554339
immunomodulatory and antioxidant potential of lactobacillus exopolysaccharides.background: immunomodulation by probiotic microorganisms has become a topic of increasing interest in food microbiology. polysaccharides are broadly used in the food industry as gelling, thickening, stabilizing, or emulsifying agents. some probiotics such as lactic acid bacteria also produce exopolysaccharides that stimulate macrophage production of cytokines. the aim of this study was to characterize the effects of exopolysaccharides of lactobacillus paracasei subsp. paracasei ntu 101 (101ep) a ...201121560134
extension of tosèla cheese shelf-life using non-starter lactic acid bacteria.six strains of non-starter lactic acid bacteria (nslab) were used to extend the shelf-life of the fresh cheese tosèla manufactured with pasteurised cows' milk. the acidification kinetics of three lactobacillus paracasei, one lactobacillus rhamnosus and two streptococcus macedonicus were studied in synthetic milk medium. lb. paracasei ndp78 and ndp88 and s. macedonicus ndp1 and pb14-1 showed an interesting acidifying capacity and were further characterised for growth in uht milk and production of ...201121569930
lactobacilli producing bispecific llama-derived anti-rotavirus proteins in vivo for rotavirus-induced diarrhea.aims: using genetically engineered lactobacilli, producing high avidity llama vhh domains (referred to as anti-rotavirus proteins; arps), to test the effect of multimeric antibody fragments as prophylaxis and therapy against rotavirus infection. methods: two arps, arp1 and arp3, shown to bind to different epitopes and act synergistically against rotavirus, were displayed on the surface of lactobacillus paracasei as monovalent or bivalent proteins (mono- or bi-specific). results: although a nonsi ...201121585264
isolation and phenotypic characterization of lactobacillus casei and lactobacillus paracasei bacteriophage-resistant mutants.aims:  to isolate and characterize bacterial strains derived from lactobacillus casei and lactobacillus paracasei strains and resistant to phage mlc-a. methods and results:  two of nine assayed strains rendered resistant mutants with recovery efficiencies of 83% (lact. paracasei atcc 27092) and 100% (lact. casei atcc 27139). dna similarity coefficients (rapd-pcr) confirmed that no significant genetic changes occurred while obtaining resistant mutants. neither parent nor mutant strains spontaneou ...201121599814
screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts.the selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. the aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, bifidobacterium longum and bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. ...201121619939
screening for lactobacilli with probiotic properties in the infant gut microbiota.lactobacilli are believed to be beneficial for the human hosts and are currently being evaluated as potentially probiotic bacteria. in this study, lactobacillus strains were isolated from infant faeces and were examined in vitro for potential probiotic properties. faecal specimens from 63 healthy, full-term infants were collected at 4, 30 and 90 days after delivery. seventy-four lactobacillus strains were isolated and one or more different phenotypes from each infant (n = 44) were selected for f ...201121621627
antiosteoporotic effects of lactobacillus -fermented soy skim milk on bone mineral density and the microstructure of femoral bone in ovariectomized mice.osteoporosis is a major skeletal disease associated with loss of estrogen in postmenopausal women. in this study, lactobacillus paracasei subsp. paracasei ntu 101 (ntu 101f) and lactobacillus plantarum ntu 102 (ntu 102f) were used as starters to ferment soy skim milk. this was then used as a nutritional supplement for 8 weeks to ovariectomized (ovx) mice. this study reveals that soy skim milk fermented with lactobacilli can increase the contents of aglycone isoflavones, soluble calcium, and vita ...201121668014
effect of bioactive compounds in lactobacilli-fermented soy skim milk on femoral bone microstructure of aging mice.background: soy skim milks fermented with lactobacilli contain various phytochemicals such as isoflavones and peptides. we used lactobacilli-fermented soy skim milk as a nutritional supplement for 6 weeks to investigate its anti-osteoporosis effect in 13-month-old female balb/c aging mice. freeze-dried powder of soy skim milk fermented by lactobacillus paracasei subsp. paracasei ntu 101 (ntu 101f) and l. plantarum ntu 102 (ntu 102f) were used in this study. results: the trabecular bone volumes i ...201121815163
lactobacillus paracasei reduces intestinal inflammation in adoptive transfer mouse model of experimental colitis.studies showed that specific probiotics provide therapeutic benefits in inflammatory bowel disease. in vitro evidence suggested that lactobacillus paracasei also called st11 (cncm i-2116) is a potent strain with immune modulation properties. however, little is known about its capacity to alleviate inflammatory symptoms in vivo in this context, the main objective of this study was to investigate the role of st11 on intestinal inflammation using the adoptive transfer mouse model of experimental co ...201121808650
inhibitory effect of oral lactobacillus against oral pathogens.aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ...201121801186
tolerance, safety, and effect on the faecal microbiota of an enteral formula supplemented with pre- and probiotics in critically ill children.: the aim of this study was to demonstrate the tolerance and safety of an enteral formula containing prebiotics/probiotics, and its effect on the faecal microbiota in critically ill children.201121788759
safety and persistence of orally administered human lactobacillus sp. strains in healthy adults.the aim of the study was to evaluate the safety and persistence of selected lactobacillus strains in the gastrointestinal tract (git) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential candidates for probiotic and biotechnological applications. in the first phase of the study, nine individuals consumed capsules containing lactobacillus gasseri 177 and e16b7, lactobacillus acidophilus 821-3, lactobacillus paracasei 317 and lactobacillus fermentu ...201121831792
diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by brazilian amerindians.microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. samples of the cotton seed and rice beverage were collected every 8-áh during the fermentation process for analysis of the microbiota present over 48-áh. the lactic acid bacteria (lab) population reached values of approximately 8.0-álog-ácfu/ml. a total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. lab (lactobaci ...201121839389
poly(dimethyl siloxane) surface modification with biosurfactants isolated from probiotic strains.depending on the final application envisaged for a given biomaterial, many surfaces must be modified before use. the material performance in a biological environment is mainly mediated by its surface properties that can be improved using suitable modification methods. the aim of this work was to coat poly(dimethyl siloxane) (pdms) surfaces with biosurfactants (bss) and to evaluate how these compounds affect the pdms surface properties. bss isolated from four probiotic strains (lactococcus lactis ...201121681946
characterization of certain bacterial strains for potential use as starter or probiotic cultures in dairy products.the present work was aimed at characterizing 12 strains of lactic acid bacteria (lab) to obtain improved potential starter or probiotic cultures that could be used for making dairy products from ewe's milk and cow's milk. eight strains with antimicrobial properties, isolated from ewe's milk and from cheese made from ewe's and/or cow's milk, were studied. they were identified as enterococcus faecalis (five strains), lactococcus lactis subsp. cremoris, leuconostoc mesenteroides, and lactobacillus ...201121819671
combined influence of fermentation and drying conditions on survival and metabolic activity of starter and probiotic cultures after low-temperature vacuum drying.the influence of low temperature vacuum drying process parameters on the survival, metabolic activity and residual water content of three different bacterial strains (lactobacillus paracasei ssp. paracasei, lactobacillus delbrueckii ssp. bulgaricus and bifidobacterium lactis) was investigated. shelf temperature and chamber pressure were varied and optimized by response surface methodology with regard to survival and residual water content. it is shown that the survival rate after low temperature ...201121723344
protection against atypical aeromonas salmonicida infection in common carp, cyprinus carpio l., by oral administration of a mixed microbial culture of lactobacillus paracasei, pichia membranifaciens and saccharomyces cereviciae.a microbial culture was prepared by co-cultivation of lactobacillus paracasei, pichia membranifaciens and saccharomyces cereviciae for 48 hr at 30 c in rice bran extract medium, supplemented with dextrose. oral administration of the resulting non-viable heat-inactivated microbial culture to common carp, cyprinus carpio l., delivered in feed for four weeks, induced effective protection against experimental atypical aeromonas salmonicida infection which causes "ulcer disease". after challenge of t ...201121685718
the effect of probiotics on faecal microbiota and genotoxic activity of faecal water in patients with atopic dermatitis: a randomized, placebo-controlled study.background: colonic microbiota is involved in the etiology of colon cancer according to several reports. studies also indicate that the microbiota differs between atopic patients and healthy subjects. objective: to evaluate whether a probiotic mix containing lactobacillus paracasei lpc-37, lactobacillus acidophilus 74-2, and bifidobacterium animalis subsp. lactis dgcc 420 can affect the microbiota and its genotoxic activity in healthy subjects and patients with atopic dermatitis (ad). methods: a ...201121963389
Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study.The present study investigated the ability of Bifidobacterium animalis ssp. lactis (BB-12®) and Lactobacillus paracasei ssp. paracasei (L. casei 431®) to modulate the immune system using a vaccination model in healthy subjects. A randomised, double-blind, placebo-controlled, parallel-group study was conducted in 211 subjects (56 % females, mean age 33·2 (sd 13·1) years). Subjects consumed a minimum of 109 colony-forming units of BB-12® (capsule) or L. casei 431® (dairy drink) or a matching place ...201121899798
Influence of mother's intestinal microbiota on gut colonization in the infant.The aim here was to elucidate the mother-infant association in the gut colonization of 1-6 month-old infants and to establish whether probiotics can influence this process. Fecal samples from 80 mother-infant pairs were analyzed at 1 month (mothers and infants) and 6 months (infants) by real-time polymerase chain reaction to assess bacterial numbers. This double-blind placebo-controlled trial involved 2 different probiotic combinations (1. Lactobacillus rhamnosus + Bifidobacterium longum and 2. ...201121983067
Bio-control of waterborne pathogens using Lactobacillus spp.Bacteria play a significant role in water contamination. Chemicals are mostly used for the treatment of bacteriologically contaminated water. The use of bacterial interactions is a new approach to limit the pathogens' growth. Detection of antimicrobial substances produced by lactic acid bacteria against the waterborne pathogens is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. Evaluation of antimicr ...201122131013
accelerated production of dry fermented sausage.the scope of this paper is to review work connected with accelerated ripening of dry fermented sausages by addition of proteolytic enzymes. an overview of the following topics is given: practical sausage experiments with addition of various proteinases of bacterial origin, including data from sensory, biochemical and gc/ms analyses; biochemical and genetic characterization of the enzyme shown to be most useful in these experiments, the serine proteinase from lactobacillus paracasei subsp. paraca ...199622060654
the spatial distribution of bacteria in grana-cheese during ripening.the microbial composition and its spatial distribution of grana trentino, a hard parmesan-like cheese, was determined, from vat milk to cheese. after cutting along the vertical axis of the cheese wheels, three layers were sampled diagonally across the cheese: under the cheese rind, an intermediate section and the cheese core. after two different ripening periods (9 and 18 months), the cheese samples were analysed using traditional culture dependent and culture independent methods. milk samples w ...201221943677
distinct immunomodulatory properties of lactobacillus paracasei strains.this study was performed to ascertain the immunomodulatory effect of lactobacillus paracasei strains. these strains were also genetically characterized.201121899667
resistance of two temperate lactobacillus paracasei bacteriophages to high pressure homogenization, thermal treatments and chemical biocides of industrial application.temperate bacteriophages ф ilp84 and ф ilp1308, previously isolated from mitomycin c-induction of lactobacillus paracasei strains 84 and cnrz1308, respectively, were tested for their resistance to several physical and chemical treatments applied in dairy industry. long-term survival at 4 °c, -20 °c and -80 °c, resistance to either thermal treatments of 63 °c, 72 °c and 90 °c, high pressure homogenization (hph, 100 mpa) or classic (ethanol, sodium hypochlorite and peracetic acid) and new commerci ...201222029923
Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products.It is well-known that probiotics have a number of beneficial health effects in humans and animals, including the reduction of symptoms in lactose intolerance and enhancement of the bioavailability of nutrients. Probiotics have showed to possess antimutagenic, anticarcinogenic and hypocholesterolemic properties. Further, they were also observed to have antagonistic actions against intestinal and food-borne pathogens, to decrease the prevalence of allergies in susceptible individuals and to have i ...201122159887
Exopolysaccharide-Overproducing Lactobacillus paracasei KB28 Induces Cytokines in Mouse Peritoneal Macrophages via Modulation of NF-kappaB and MAPKs.Exopolysaccharides (EPSs) are microbial polysaccharides that are released outside of the bacterial cell wall. There have been few studies on EPS-producing lactic acid bacteria that can enhance macrophage activity and the underlying signaling mechanism for cytokine expression. In the current study, EPS-overproducing Lactobacillus (L.) paracasei KB28 was isolated from kimchi and cultivated in conditioned media containing glucose, sucrose, and lactose. The whole bacterial cells were obtained with t ...201122127129
the effect of using different probiotic cultures on conjugated linoleic acid (cla) concentration and fatty acid composition of white pickle cheese.pickle white cheeses were produced from whole milk with five different probiotic cultures (enterococcus faecium, lactobacillus paracasei, bifidobacterium longum, bifidobacterium bifidum and lactobacillus acidophilus). conjugated linoleic acid (cla) content of cheeses ranged from 3.52 to 3.92 mg/g. probiotic differences and storage process have not affected the cla contents of the samples statistically. there was no correlation between the cla content of all probiotic cheeses and saturated fatty ...201122166042
a combination of calcium phosphate and probiotics beneficially influences intestinal lactobacilli and cholesterol metabolism in humans.background & aims: the study focuses on the influence of a probiotic supplement alone and in combination with a calcium supplement on faecal lactobacilli colonisation and beneficial health effects such as a lowering of blood cholesterol. methods: thirty-two men and women participated in the double-blind, placebo-controlled, cross-over study. all participants consumed a probiotic drink containing 10(10)cfu/d lactobacillus paracasei (lpc37) for four weeks. in addition, one group consumed bread enr ...201122019281
beneficial effects of a strain of lactobacillus paracasei subsp. paracasei in staphylococcus aureus-induced intestinal and colonic injury.the aim of this study was to investigate the in vitro and in vivo anti-staphylococcal activity of a lactic acid bacterial strain and its effect on the intestinal histological damage caused by staphylococcus aureus infection.201121963346
treatment and secondary prevention effects of the probiotics lactobacillus paracasei or bifidobacterium lactis on early infant eczema: randomized controlled trial with follow-up until age 3 years.background: allergic disease has been associated with altered intestinal microbiota. therefore, probiotics have been suggested as a potential treatment for eczema. objective: we investigated whether dietary supplementation of infants with eczema at age 3-6 months with lactobacillus paracasei cncm i-2116 or bifidobacterium lactis cncm i-3446 had a treatment effect or altered allergic disease progression. methods: primary outcome included eczema severity (scoring atopic dermatitis, scorad) 3 month ...201122092692
lactobacillus brantae sp. nov., isolated from feces of canada geese (branta canadensis).three strains of lactic acid bacteria (lab) were isolated from feces of apparently healthy wild canada geese (branta canadensis) in 2010 using the cultivation of fecal lab on rogosa sl agar under aerobic conditions. these three isolates were found to share 99.9% sequence similarity of their 16s rrna, the 16s-23s intergenic transcribed spacer region (its), partial 23s rrna, rpob, rpoc, rpoa, and phes gene sequences. however, at the same time these strains exhibited lower levels of sequence simila ...201122021580
To the editor: probiotic Lactobacillus paracasei effect on cariogenic bacterial flora. 201122205267
Comparison of different IlvE aminotransferases in Lactobacillus sakei and investigation of their contribution to aroma formation from branched chain amino acids.Branched chain aminotransferases (IlvE/BcaT), specific for leucine, isoleucine, and valine initialize the formation of methyl-branched volatiles, which are strongly linked to the typical aroma of cured meat products. Lactobacillus sakei, one of the dominating lactic acid bacteria species for meat fermentations and commonly used as starter lacks this enzyme, whereas the presence of IlvE has been reported for Lactobacillus paracasei, a non-starter lactic acid bacterium occurring in meat products a ...201222202874
Growth of Lactobacillus paracasei ATCC 334 in a cheese model system: a biochemical approach.Growth of Lactobacillus paracasei ATCC 334, in a cheese-ripening model system based upon a medium prepared from ripening Cheddar cheese extract (CCE) was evaluated. Lactobacillus paracasei ATCC 334 grows in CCE made from cheese ripened for 2 (2mCCE), 6 (6mCCE), and 8 (8mCCE) mo, to final cell densities of 5.9×10(8), 1.2×10(8), and 2.1×10(7)cfu/mL, respectively. Biochemical analysis and mass balance equations were used to determine substrate consumption patterns and products formed in 2mCCE. The ...201122032349
Biodiversity and growth dynamics of lactic acid bacteria in artisanal PDO Ossau-Iraty cheeses made from raw ewe's milk with different starters.The biodiversity and growth dynamics of Lactic Acid Bacteria (LAB) in farm-house Ossau-Iraty cheeses were investigated from vat milk to 180 days of ripening in six independent batches made from six raw ewe's milks using five typical cheese-making methods. Commercial starter S1 was used for three batches, starter S1 combined with S2 for one batch and no starter for two batches. Up to ten LAB species from five genera and up to two strains per species were identified per milk; up to eleven species ...201222029916
Oral and faecal lactobacilli and their expression of mannose-specific adhesins in individuals with and without IgA deficiency.Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 16S rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of contr ...201222018813
recombinant probiotic expressing listeria adhesion protein attenuates listeria monocytogenes virulence in vitro.listeria monocytogenes, an intracellular foodborne pathogen, infects immunocompromised hosts. the primary route of transmission is through contaminated food. in the gastrointestinal tract, it traverses the epithelial barrier through intracellular or paracellular routes. strategies to prevent l. monocytogenes entry can potentially minimize infection in high-risk populations. listeria adhesion protein (lap) aids l. monocytogenes in crossing epithelial barriers via the paracellular route. the use o ...201222235279
[quorum-sensing behavior of lactobacillus paracasei hd1.7].paracin 1.7 was a bacteriocin extracted from lactobacillus paracasei hd1.7. in this study, we investigated quorum-sensing mechanism that regulates the metabolism of paracin 1.7 in strain hd1.7.201122260055
[viability and efects of the probiotic lactobacillus aracaseissp aracasei in chilean low-fat gauda cheese].the objective of this study is to infer the survival of the probiotic lactobacillus paracasei ssp paracasei added during the processing of low-fat gouda cheese, during the maturation (21 days) and the commercialization (14 days), in order to see the influence that this organism has on the quality of the product. the treatments were: ti (control): gouda with normal fat content; t2: low fat gouda cheese (qgs) t3: qgs, with additional probiotic added with the initial culture; t4: qgs, with the prob ...201123094525
characterization and purification of a bacteriocin from lactobacillus paracasei subsp. paracasei bmk2005, an intestinal isolate active against multidrug-resistant pathogens.a strain of lactobacillus paracasei subsp. paracasei bmk2005 isolated from healthy infant faeces has shown a remarkable antibacterial activity against 32 bacterial pathogenic strains of human clinical isolates. among them, 13 strains belonging to species of escherichia coli, citrobacter freundii, citrobacter diversus, klebsiella oxytoca, enterobacter cloacae and pseudomonas aeruginosa were resistant to cefotaxime (ctx) and ceftazidime (caz), and 4 strains of staphylococcus aureus were resistant ...201122805936
technological and probiotic role of adjunct cultures of non-starter lactobacilli in soft cheeses.the influence of two cheese-isolated lactobacillus strains on cheese composition, acceptability and probiotic capacity was assessed. soft cheeses with and without the addition of lactobacillus plantarum i91 or lactobacillus paracasei i90 were prepared. gross composition was assessed and secondary proteolysis was described by soluble fractions and free amino acids profiles. acceptability was determined by a panel of 98 non-trained consumers. cheeses harboring added lactobacillus strains were also ...201122265282
lactobacillus paracasei lp6 favors immune modulation induced by allergoid treatment in ragweed sensitized mice.it has been hypothesized that lactic acid bacteria (lab) could be used as adjuvant for specific immunotherapy (sit), as various studies conducted on humans and animals converge to define lab as anti-th2 modulators and treg inducers. in the present study we evaluated the effects of lab, in particular lactobacillus paracasei lp6 (lp6), in a mouse model of ragweed (rw) allergy. groups of balb/c mice, experimentally sensitized towards ragweed, were treated by viable lp6 or by rwallergoid with or wit ...201122230395
a comprehensive approach to determine the probiotic potential of human-derived lactobacillus for industrial use.specific strains should only be regarded as probiotics if they fulfill certain safety, technological and functional criteria. the aim of this work was to study, from a comprehensive point of view (in vitro and in vivo tests), three lactobacillus strains (lactobacillus paracasei jp1, lactobacillus rhamnosus 64 and lactobacillus gasseri 37) isolated from feces of local newborns, determining some parameters of technological, biological and functional relevance. all strains were able to adequately g ...201223498174
applied development of crude enzyme from bacillus cereus in prebiotics and microbial community changes in soil.the chitosanase and chitinase activity were revealed in the culture supernatant of bacillus cereus tku027 with shrimp head powder (shp) as the sole carbon/nitrogen source. the chitosan with 60% degree of deacetylation (dd) was depolymerized by tku027 crude enzyme. the low dp oligomers stimulated the growth of lactobacillus paracasei bcrc12193 and lactobacillus kefir bcrc14011 in a mrs broth supplemented with low dp oligomers for 12 h. conversely, the high dp oligomers (0.1%) had potent inhibitor ...201223399269
effects of probiotics and antibiotics on the intestinal homeostasis in a computer controlled model of the large intestine.antibiotic associated diarrhea and clostridium difficile infection are frequent complications of broad spectrum antibiotic therapy. probiotic bacteria are used as therapeutic and preventive agents in these disorders, but the exact functional mechanisms and the mode of action are poorly understood. the effects of clindamycin and the probiotic mixture vsl#3 (containing the 8 bacterial strains streptococcus thermophilus, bifidobacterium breve, bifidobacterium longum, bifidobacterium infantis, lacto ...201222452835
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