| effect of roasting and fermentation on viscosity of cereal-legume based food formulas. | with the view of preparing semi-liquid weaning foods of high energy content, the influence of roasting (stationary hot air treatment) and fermentation (with natural and pure culture inocula) on the viscosity of maize-sorghum-soya porridges was investigated. roasting resulted in porridges of significantly higher viscosity (cooked porridge cooled to 40 degrees c). porridges made from the individual ingredients (maize, sorghum, soya) did not show this behaviour. natural fermentation of mixed ingred ... | 1994 | 7855078 |
| cloning, nucleotide sequence, and transcriptional analysis of the pediococcus acidilactici l-(+)-lactate dehydrogenase gene. | recombinant plasmids containing the pediococcus acidilactici l-(+)-lactate dehydrogenase gene (ldhl) were isolated by complementing for growth under anaerobiosis of an escherichia coli lactate dehydrogenase-pyruvate formate lyase double mutant. the nucleotide sequence of the ldhl gene predicted a protein of 323 amino acids showing significant similarity with other bacterial l-(+)-lactate dehydrogenases and especially with that of lactobacillus plantarum. the ldhl transcription start points in p. ... | 1995 | 7887607 |
| agar plate method using lactobacillus plantarum for biotin determination in serum and urine. | an improved agar plate method of biotin bioassay using lactobacillus plantarum atcc 8014 and bromocresol purple was established to determine biotin levels in human serum and urine. samples were treated with 4.5 n h2so4 to liberate free biotin, autoclaved for 1 h and neutralized by 4.5 n naoh, then 10 microliters was added to wells in each plate. the biotin levels were measured in 190 serum and 59 urine samples, and the means were 2.7 +/- 0.53 ng/ml and 12.4 +/- 5.56 ng/mg of creatinine, respecti ... | 1994 | 7891209 |
| sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp. | we have shown previously that the copy number of plasmid psy10 from the marine cyanobacterium synechococcus sp. nkbg 042902 is dependent on the salinity of the growth medium. we report here the complete nucleotide sequence (2561 bp) of this plasmid. the longest open reading frame, orf-b (1.08 kb), occurs on a 1.6-kb ecori fragment. this orf encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pca2.4 from synechocystis sp. str ... | 1994 | 7899510 |
| determination of chromosome size and number of rrn loci in lactobacillus plantarum by pulsed-field gel electrophoresis. | the size of the lactobacillus plantarum ccm 1904 chromosome was determined by pulse-field gel electrophoresis. it was found to be 3.3-3.4 mb using sfii or asci restriction endonucleases, compared to 3-4 mb found for the other l. plantarum strains tested. l. plantarum ccm 1904 5s rdna was clonedl by polymerase chain reaction, sequenced, and used as a probe to characterize strains. at least five rrn loci were found. the pulsed-field gel electrophoresis macrorestriction patterns were strain-specifi ... | 1994 | 7914501 |
| cloning and expression of the plasmid encoded beta-d-galactosidase gene from a lactobacillus plantarum strain of dairy origin. | the beta-galactosidase (beta-gal) gene from lactobacillus plantarum c3.8 was cloned and expressed in lactococcus lactis and escherichia coli. hybridization experiments indicated that the gene is located on a plasmid and is present in other strains of lactobacillus plantarum. its sequence is very similar to a leuconostoc lactis beta-gal gene. expression of the gene, both in lactobacillus plantarum and in lactococcus lactis, was four-fold higher in cells growth in lactose compared to those grown i ... | 1994 | 7958766 |
| essential role of arginine 235 in the substrate-binding of lactobacillus plantarum d-lactate dehydrogenase. | substitutions of the conserved arg-235 with lys and gln induced drastic decreases in the catalytic efficiency of lactobacillus plantarum d-lactate dehydrogenase (d-ldh). both the mutant enzymes showed a marked resistance to 2,3-butanedione, by which the wild-type enzyme is rapidly inactivated unless nadh and oxamate are present. the pka of the catalytic his was markedly shifted to the alkaline side by the arg-to-gln substitution, while it was not significantly shifted by the arg to lys substitut ... | 1994 | 7961609 |
| development of an amylolytic lactobacillus plantarum silage strain expressing the lactobacillus amylovorus alpha-amylase gene. | an amylolytic lactobacillus plantarum silage strain with the starch-degrading ability displayed by lactobacillus amylovorus was developed. an active fragment of the gene coding for alpha-amylase production in l. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain l. plantarum lp80 at the cbh locus. the alpha-amylase gene fragment was also introduced into l. plantarum lp80 on an autoreplicative plasmid. both constructions were also performed in the laborat ... | 1994 | 7986030 |
| effect of processing conditions on acidification properties of wheat sour doughs. | the ph, total titratable acidity (tta) and lactic and acetic acids production have been investigated for wheat sour doughs with and without yeast addition, inoculated with two strains of lactobacillus plantarum (b33, b39). to study the effect of flour extraction rate (0.54, 11.11 and 1.68% ash content), dough yield (dy) (160, 200 and 240 of sour dough/100 g flour), and fermentation temperature (25, 30 and 35 degrees c) a response surface regression, factor analysis and k-means clustering analysi ... | 1994 | 7986676 |
| permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes. | the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ... | 1994 | 8000549 |
| use of homologous expression-secretion signals and vector-free stable chromosomal integration in engineering of lactobacillus plantarum for alpha-amylase and levanase expression. | the genuine alpha-amylase gene from bacillus licheniformis (amyl) is not expressed in lactobacillus plantarum, but replacement of the amyl promoter by a strong l. plantarum promoter leads to efficient expression of the gene and secretion of more than 90% of the alpha-amylase into the culture supernatant. a series of l. plantarum genetic cassettes (transcription and translation with or without secretion) were cloned by translation fusion of random dna fragments to the silent amyl coding frame in ... | 1994 | 8017927 |
| detection, purification, and partial characterization of plantaricin c, a bacteriocin produced by a lactobacillus plantarum strain of dairy origin. | a bacteriocin produced by lactobacillus plantarum ll441 was selected from the inhibitory products of 75 mesophilic lactobacilli because of its potency and broad spectrum. it is a peptide of 3.5 kda whose amino-terminal sequence is nh2-k-k-t-k-k-n-x-s-g-d-i-. it is bactericidal and, in some cases, bacteriolytic. the peptide, called plantaricin c, retained its activity after boiling, storage, and treatment at different phs. | 1994 | 8080262 |
| the gene encoding plantaricin a, a bacteriocin from lactobacillus plantarum c11, is located on the same transcription unit as an agr-like regulatory system. | purification and amino acid sequencing of plantaricin a, a bacteriocin from lactobacillus plantarum c11, revealed that maximum bacteriocin activity is associated with the complementary action of two almost-identical peptides, alpha and beta (j. nissen-meyer, a. g. larsen, k. sletten, m. daeschel, and i. f. nes, j. gen. microbiol. 139:1973-1978, 1993). a 5-kb chromosomal hindiii restriction fragment containing the structural gene of plantaricin a was cloned and sequenced. only one gene encoding p ... | 1994 | 8117074 |
| biochemical changes during the preservation stage of ripe olive processing. | the influences of initial sodium chloride (6% and 0% w/v in tap water) and acetic acid concentrations (0.3%, and 0.6% v/v), use of starter culture, and aerobic versus anaerobic conditions on the biochemical changes that take place throughout the preservation stage of ripe olive processing were investigated. glucose, fructose and sucrose were completely consumed during preservation. mannitol and malic acid were metabolized only in the presence of lactic acid bacteria or oxidative yeast (aerobic t ... | 1993 | 8121471 |
| microbial and nutritional aspects of feeding fermented feed (poultry by-products) to pigs. | broiler by-products (heads, feet, and viscera) mixed with 4% dextrose were pasteurized for 4 min at 90 degrees c core temperature, cooled to 20 degrees c, and fermented with lactobacillus plantarum as starter culture. these fermented poultry by-products were fed to 12 individually housed fattening pigs as part (17.6% of the dry matter) of their fattening ration, the remainder composed of compound pig feed. control pigs received a compound pig feed only. both groups of pigs were fed restrictively ... | 1993 | 8122350 |
| the aggregation of human platelets by lactobacillus species. | the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ... | 1993 | 8126421 |
| unmodified and recombinant strains of lactobacillus plantarum are rapidly lost from the rumen by protozoal predation. | a genetically-manipulated strain of lactobacillus plantarum and the unmodified parent strain were introduced into the rumen of sheep at an initial inoculum level of 1 x 10(7) cfu ml-1 of rumen fluid. there were no significant differences between the viable counts of the two inoculants throughout a 24 h sampling period. the rates of loss were 0.36 and 0.29 h-1 (proportion of colony-forming units lost, measured over the first 2 h) for the parent strain and recombinant strain respectively, and with ... | 1994 | 8144412 |
| the refined structures of a stabilized mutant and of wild-type pyruvate oxidase from lactobacillus plantarum. | the crystal structure of pyruvate oxidase (ec 1.2.3.3) from lactobacillus plantarum stabilized by three point mutations has been refined at 2.1 a resolution using the simulated annealing method. based on 87,775 independent reflections in the resolution range 10 to 2.1 a, a final r-factor of 16.2% was obtained at good model geometry. the wild-type enzyme crystallizes isomorphously with the stabilized enzyme and has been analyzed at 2.5 a resolution. pyruvate oxidase is a homotetramer with point g ... | 1994 | 8145244 |
| vitamin and amino acid requirements of lactobacillus plantarum strains isolated from green olive fermentations. | the requirement for essential amino acids and vitamins was determined in wild-type lactobacillus plantarum strains isolated from green olive fermentation brines. all the strains were found to be auxotrophic with respect to the amino acids but some of them were prototrophic for pyridoxal, p-aminobenzoic acid and/or nicotinic acid. their growth response to these nutrients was also studied and found to be quite heterogeneous. nutritional requirement pattern as a criteria for selecting starter cultu ... | 1994 | 8200862 |
| antagonistic activity of two strains of lactic acid bacteria against listeria monocytogenes and yersinia enterocolitica in a model fish product at 5 degrees c. | the antagonistic activity of lactic acid bacteria against one strain of listeria monocytogenes, scott a and one strain of yersinia enterocolitica serotype o:3 was studied by using either a strain of a leuconostoc spp. or a lactobacillus plantarum strain. each of the two potential pathogenic, psychrotrophic bacteria were inoculated with either of the two lactic acid bacteria in sterile shrimp extracts containing citric acid (ph 5.8 and 6.0) and in some experiments also 3% (w/v) nacl. the samples ... | 1993 | 8217515 |
| a study of five bacteriophages of the myoviridae family which replicate on different gram-positive bacteria. | a comparative study is reported on five phages of the myoviridae family which propagate on bacillus subtilis, b. thuringiensis, enterococcus sp., lactobacillus plantarum, or staphylococcus aureus. the phages are morphologically identical and characterized by isometric heads with conspicuous capsomers and by contractile tails with complex base plates. the phages show similar protein profiles, but vary considerably in burst size. phage dnas are about 95-166 kb in size and are unrelated by dna-dna ... | 1993 | 8240019 |
| microflora of the aerobic preservation of directly brined green olives from hojiblanca cultivar. | new procedures for the preservation stage of ripe olives from hojiblanca cultivar were studied. an aerobic fermentative process was used with initial ph correction (0.3% acetic acid) and various nacl concentrations: 6, 3 and 0% (w/v) in tap water. treatments were carried out at industrial level and the spontaneous changes monitored. at initial salt concentrations of 6 and 3% (w/v) nacl, ph rose progressively, reaching 4.3 at equilibrium maintaining during this period a constant free lactic acidi ... | 1993 | 8244900 |
| biosynthesis of biotin from dethiobiotin by the biotin auxotroph lactobacillus plantarum. | lactobacillus plantarum requires biotin for growth. we show that in the presence of high levels of the biotin biosynthetic precursor, dethiobiotin, l. plantarum synthesizes biotin and grows in medium with dethiobiotin but without biotin. lactobacillus casei also grew under similar conditions. | 1993 | 8244941 |
| purification and characterization of plantaricin a, a lactobacillus plantarum bacteriocin whose activity depends on the action of two peptides. | a lactobacillus plantarum bacteriocin, plantaricin a, has been purified to homogeneity by ammonium sulphate precipitation, binding to cation exchanger and octyl-sepharose, and reverse-phase chromatography. the bacteriocin activity was associated with two peptides, termed alpha and beta, which were separated upon reverse-phase chromatography. bacteriocin activity required the complementary action of both the alpha and beta peptides. from the n-terminal end, 21 and 22 amino acid residues of alpha ... | 1993 | 8245827 |
| lactobacillus plantarum ldhl gene: overexpression and deletion. | lactobacillus plantarum is a lactic acid bacterium that converts pyruvate to l-(+)- and d-(-)-lactate with stereospecific enzymes designated l-(+)- and d-(-)-lactate dehydrogenase (ldh), respectively. a gene (designated ldhl) that encodes l-(+)-lactate dehydrogenase from l. plantarum dg301 was cloned by complementation in escherichia coli. the nucleotide sequence of the ldhl gene predicted a protein of 320 amino acids closely related to that of lactobacillus pentosus. a multicopy plasmid bearing ... | 1994 | 8300514 |
| behaviour of listeria monocytogenes during the maturation of naturally and artificially contaminated salami: effect of lactic-acid bacteria starter cultures. | the antimicrobial activity of a bacteriocin-producing lactobacillus plantarum mcs strain against listeria monocytogenes in naturally and artificially contaminated salami was evaluated in comparison with that of other non bacteriocin-producing lactic-acid bacteria (i.e., l. plantarum mcs1 mutant strain and two commercial starter cultures). in salami not inoculated with lactic-acid bacteria, moderate l. monocytogenes growth was observed after 7-14 days. the addition of the starter cultures prevent ... | 1993 | 8312141 |
| potential application of laser/microbe bioassay technology for determining water-soluble vitamins in foods. | a microbiological technique was developed for quantitating niacin by determining microbial growth rates in response to the amount of vitamin available. unlike the current official aoac method, the new procedure for niacin measured the growth rates during the early exponential growth phase rather than during the stationary phase. lactobacillus plantarum was used to determine niacin to a lower limit of 100 pg/ml. the assay time was approximately 6 h, compared with 16-24 h for the current aoac meth ... | 1993 | 8318864 |
| characteristics and antagonistic activity of lactic acid bacteria isolated from chilled fish products. | in fish products, contrary to other foods, the commercial use of starter cultures is relatively unexploited. in this study, 61 isolates of lactic acid bacteria from lightly preserved, chilled fish products were characterized with special emphasis on their antagonistic activity and their possible use as starter cultures. the strains were phenotypically identified and characterized with regard to gas production, carbohydrate fermentations, production of off odours, h2o2-production, antagonistic ac ... | 1993 | 8347429 |
| histidine 296 is essential for the catalysis in lactobacillus plantarum d-lactate dehydrogenase. | two his residues, his-205 and his-296, in lactobacillus plantarum d-lactate dehydrogenase are highly conserved in the d-isomer-specific 2-hydroxyacid dehydrogenase family, suggesting that they are candidates for the catalytic his in the enzyme. the substitution of his-296 with tyr by means of site-directed mutagenesis induced a drastic decrease in the reaction rate, while a his-205 to tyr substitution induced no large change in the catalytic properties. in pyruvate reduction, the tyr-296 enzyme ... | 1993 | 8349682 |
| analysis of intestinal flora of a patient with congenital absence of the portal vein. | a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ... | 1993 | 8364525 |
| isoprenoid biosynthesis in bacteria: two different pathways? | the biosynthesis of isopentenylpyrophosphate, a central intermediate of isoprenoid formation, was investigated in six different bacterial organisms. cell-free extracts of myxococcus fulvus, staphylococcus carnosus, lactobacillus plantarum and halobacterium cutirubrum converted [14c]acetyl-coa or [14c]hydroxymethylglutaryl-coa to [14c]mevalonic acid. furthermore, [14c]mevalonic acid, [14c]mevalonate-5-phosphate and [14c]mevalonate-5-pyrophosphate were metabolized to [14c]isopentenylpyrophosphate. ... | 1993 | 8405922 |
| fermentation of raw poultry byproducts for animal nutrition. | in this study, the fermentation of raw, inedible poultry byproducts mixed with sugarbeet pulp and dextrose and inoculated with lactobacillus plantarum and(or) enteroccocus faecium resulted in a drop of ph in the byproducts to approximately 4.0 to 4.5 within 48 h. to keep the fermented product stable for a period of 21 d, the addition of > or = 3% (wt/wt) of a fermentable carbohydrate was necessary. with a high inoculation level of approximately 10(8) to 10(9) l. plantarum per gram, or with acidi ... | 1993 | 8407654 |
| the feeding of raw, fermented poultry byproducts: using mink as a model. | in this study, the safety of fermentation as a method of preservation of raw animal byproducts used for animal nutrition was tested. two feeding trials with mink, as a model for nonruminant animals, were carried out. in the first trial mink were given a fermented diet composed of raw poultry and fish byproducts supplemented with cereals, glucose, lactic acid, premix, and starter culture (lactobacillus plantarum and enterococcus faecium). these mink failed to deliver kits, and 7 of the 30 females ... | 1993 | 8407655 |
| antimicrobial activity of lactic acid bacteria isolated from sour doughs: purification and characterization of bavaricin a, a bacteriocin produced by lactobacillus bavaricus mi401. | three hundred and thirty-five lactic acid bacteria were isolated from sour doughs and screened for antagonistic activity. of these 145 showed activity against one or several of the indicator strains used in the screening. the antimicrobial activity of 18 isolates were due to a proteinaceous compound. these 18 isolates belonged to three different lactobacillus species: lactobacillus bavaricus, lactobacillus curvatus and lactobacillus plantarum. the spectrum of antimicrobial activity for the three ... | 1993 | 8407671 |
| bacteriocinogenic activity of lactobacilli from fermented sausages. | during the screening of the inhibitory activity of 254 strains of lactobacilli isolated from fermented sausages at different times of ripening, 22% of the strains showed inhibition that was not related to acid or hydrogen peroxide, towards one or more indicator strains. not all the strains were capable of secreting the inhibitory compound in the supernatant fluid. the characterization of the inhibitory compound from three strains showed that they were bacteriocins with a bactericidal mode of act ... | 1993 | 8407674 |
| detection of bacteriocins produced by lactobacillus plantarum strains isolated from different foods. | bacterial strains (106 in toto) isolated from different foods and identified as lactobacillus plantarum were screened for antagonistic activities against other bacteria under conditions which eliminated the effects of organic acids and hydrogen peroxide. five isolates were shown to be bacteriocin producers, and the bacteriocins, on the basis of their host range inhibition and cross inhibition were all different. the bacteriocins were preliminarily characterized by temperature stability, sensitiv ... | 1993 | 8412846 |
| structure of the thiamine- and flavin-dependent enzyme pyruvate oxidase. | pyruvate oxidase from lactobacillus plantarum is a tetrameric enzyme that decarboxylates pyruvate, producing hydrogen peroxide and the energy-storage metabolite acetylphosphate. structure determination at 2.1 angstroms showed that the cofactors thiamine pyrophosphate (tpp) and flavin adenine dinucleotide (fad) are bound at the carboxyl termini of six-stranded parallel beta sheets. the pyrophosphate moiety of tpp is bound to a metal ion and to a beta alpha alpha beta unit corresponding to an esta ... | 1993 | 8438155 |
| molecular analysis of the rolling-circle replicating plasmid pa1 of lactobacillus plantarum a112. | lactobacillus plantarum a112 has four different plasmids. plus-origin-specific probes were used to determine that the smallest, cryptic plasmid, pa1 (2,820 bp), showed homology to the pe194 plasmid family. this subclass of plasmids uses the rolling-circle mode of replication. subsequent analysis of plasmid pa1 demonstrated that it generates single-stranded dna intermediates, and sequence analysis revealed that it contains three putative open reading frames (orfs): orf1, orf2, and orf3, which cou ... | 1993 | 8439153 |
| effects of gluconodeltalactone and lactobacillus plantarum on the production of histamine and tyramine in fermented sausages. | fermented sausages were made experimentally with addition of 0.3% (w/w) gluconodeltalactone (gdl) or a starter culture of lactobacillus plantarum, unable to produce histamine or tyramine. controls were produced without gdl and starter culture. during 15 days preparation and storage periods, number of bacteria and lactobacilli, ph, and levels of histamine, tyrosine and tyramine were monitored, and organoleptic evaluations were carried out. maximal histamine levels were 19.41 micrograms/g of dry m ... | 1993 | 8466803 |
| numerical taxonomy of lactobacillus spp. associated with healthy and diseased mucosa of the human intestines. | two-hundred and fifty lactobacillus strains isolated from healthy and diseased mucosa of human intestines of 75 individuals and 49 reference strains were phenotypically classified using 49 unit characters. data were processed by the jaccard (sj) and simple matching (ssm) coefficients, and unweighted pair group algorithm with arithmetic averages. seventeen major clusters were defined at the 76% sj-similarity level which approximately correspond to the ssm-level of 91%. seven clusters could be ide ... | 1993 | 8468264 |
| sugar uptake and involved enzymatic activities by yeasts and lactic acid bacteria: their relationship with breadmaking quality. | the uptake kinetics of sugars present in wheat doughs and alpha-glucosidase as well as beta-fructosidase activities were determined in different strains of yeasts and lactic acid bacteria. these strains were previously selected according to their breadmaking quality. saccharomyces cerevisiae (p6), candida guilliermondii (p40), lactobacillus plantarum (b31 and la18) and l. brevis (b21) showed good performance, while sacch. fructuum (p43), l. cellobiosus (b37) and enterococcus faecium (b11) yielde ... | 1993 | 8494688 |
| isolation and partial characterization of three rumen lactobacillus plantarum bacteriophages. | the first isolation of lactobacillus plantarum bacteriophages from ruminal fluid is reported. three bacteriophages were characterized on the basis of plaque morphology, host ranges, stability, electron microscopic morphology and dna restriction endonuclease digestion patterns. they formed clear plaques and are placed in group a of bradley's scheme and have identical host ranges. bacteriophages were stable to urea and chloroform. they were relatively thermostable but partially inactivated by rume ... | 1993 | 8510572 |
| gene disruption in lactobacillus plantarum strain 80 by site-specific recombination: isolation of a mutant strain deficient in conjugated bile salt hydrolase activity. | a chloramphenicol-resistance gene (cml) was introduced into the lactobacillus plantarum gene encoding conjugated bile acid hydrolase (cbh) on a cole1 replicon. this plasmid which is nonreplicative in lactobacillus was used to transform l. plantarum strain 80. a homologous double cross-over recombination event resulted in replacement of the chromosomal cbh gene by the cml-containing cbh gene. the transformants obtained were unable to synthesize active conjugated bile acid hydrolase (cbh). the cbh ... | 1993 | 8510654 |
| purification and partial amino acid sequence of plantaricin s, a bacteriocin produced by lactobacillus plantarum lpco10, the activity of which depends on the complementary action of two peptides. | plantaricin s, one of the two bacteriocins produced by lactobacillus plantarum lpco10, which was isolated from a green-olive fermentation (r. jiménez-díaz, r.m. ríos-sánchez, m. desmazeaud, j.l.ruiz-barba, and j.-c. piard, appl. environ. microbiol. 59:1416-1424, 1993), has been purified to homogeneity by ammonium sulfate precipitation, by binding to sp-sepharose fast-flow, phenyl-sepharose cl-4b, and c2/c18 reverse-phase chromatographies. the purification resulted in a final yield of 91.6% and a ... | 1995 | 8534111 |
| osmotic regulation of intracellular solute pools in lactobacillus plantarum. | bacteria respond to changes in medium osmolarity by varying the concentrations of specific solutes in order to maintain constant turgor pressure. the cytoplasmic pools of k+, proline, glutamate, alanine, and glycine of lactobacillus plantarum atcc 14917 increased when the osmolarity of the growth media was raised from 0.20 to 1.51 osmol/kg by kcl. when glycine-betaine was present in a high-osmolarity chemically defined medium, it was accumulated to a high cytoplasmic concentration, while the con ... | 1996 | 8550485 |
| epr polarization studies on mn catalase from lactobacillus plantarum. | the binuclear manganese active site of mn catalase catalyzes redox disproportionation of hydrogen peroxide, forming dioxygen and water. we report here multifrequency epr and microwave polarization studies of the catalytically active homovalent mn2+ complex of lactobacillus plantarum mn catalase, resolving spectra from each of the thermally accessible multiplet states of the coupled complex by multivariate methods. the experimental spectra have been simulated using computational approaches for th ... | 1996 | 8555195 |
| the antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation. | a total of 241 lactic acid bacteria belonging to lactobacillus plantarum, pediococcus pentosaceus, lactobacillus fermentum/reuteri and lactobacillus brevis from various processing stages of maize dough fermentation were investigated. results indicated that each processing stage has its own microenvironment with strong antimicrobial activity. about half of the lact. plantarum and practically all of the lact. fermentum/reuteri investigated were shown to inhibit other gram-positive and gram-negativ ... | 1995 | 8567490 |
| studies on bacteriocinogenic lactobacillus isolates from selected nigerian fermented foods. | ten bacteriocin-producing (bacteriocinogenic) lactobacillus isolates obtained from three nigerian fermented foods namely: kenkey, ogi and wara were tested against the following indicator organisms: lactobacillus plantarum and food borne pathogens comprising enterotoxigenic escherichia coli, enterohaemorrhagic escherichia coli, serratia, pseudomonas, vibrio cholerae, aeromonas sobria, aeromonas cavice, salmonella typhimurium, plesiomonas shigelloides and staphylococcus aureus. all the bacteriocin ... | 1995 | 8568643 |
| glycine betaine fluxes in lactobacillus plantarum during osmostasis and hyper- and hypo-osmotic shock. | bacteria respond to changes in medium osmolarity by varying the concentrations of specific solutes in order to maintain constant turgor. the primary response of lactobacillus plantarum to an osmotic upshock involves the accumulation of compatible solutes such as glycine betaine, proline, and glutamate. we have studied the osmotic regulation of glycine betaine transport in l. plantarum by measuring the overall and unidirectional rates of glycine betaine uptake and exit at osmostasis, and under co ... | 1996 | 8626562 |
| nutritional support to prevent and treat multiple organ failure. | enteral nutrition (en) has several advantages over parenteral nutrition (pn) for postoperative/posttrauma patients. modern technologies for tube-feeding have made early en possible. jejunal tube-feeding has advantages over gastric tube-feeding: faster metabolic recovery, less vomiting, and less risk of regurgitation and aspiration. immediate or early en stimulates the splanchnic and hepatic circulations, improves mucosal blood flow, prevents intramucosal acidosis and permeability disturbances, a ... | 1996 | 8662138 |
| the effects of lactobacillus strains and oat fiber on methotrexate-induced enterocolitis in rats. | administration of methotrexate to rats on an elemental diet results in severe enterocolitis and death. lactobacilli, an integral part of the healthy gastrointestinal microecology, may provide therapeutic benefits to help the recovery from enterocolitis. the purpose of this study was to evaluate the effects of lactobacilli and oatbase on methotrexate-induced enterocolitis in rats. | 1996 | 8690198 |
| comparative assessment of bacterial inoculation and propionic acid treatment of aerobic stability and microbial populations of ensiled high-moisture ear corn. | high-moisture ear corn (hmec) was untreated, treated with propionic acid (pa), or inoculated with a mixture of lactobacillus plantarum and enterococcus faecium and allowed to ensile in laboratory silos for 0, 7, 21, 42, 138, or 202 d. the silages were evaluated for fermentation quality, microbial populations, and aerobic stability. in all treatments, silage ph declined rapidly within 7 d, but the rate of decline seemed greatest with the inoculum. the lactic acid content of inoculated hmec was hi ... | 1996 | 8690682 |
| positive selection, cloning vectors for gram-positive bacteria based on a restriction endonuclease cassette. | lactococcus lactis contains numerous restriction and modification (r/m) systems of different specificities. a novel iis type r/m system encoded by the llai operon has previously been characterized from the l. lactis conjugative plasmid ptr2030. the llai operon is composed of six genes: first, a small regulatory gene llaic precedes the methylase gene llaim. the following three genes, llai.1, llai.2, llai.3, are all essential for restriction endonuclease activity and are designed as the restrictio ... | 1996 | 8693025 |
| the potential of lactobacillus as a carrier for oral immunization: development and preliminary characterization of vector systems for targeted delivery of antigens. | oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms (gerritse et al., 1990, 1991). the adjuvant function of different lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. after i.p. injection of trinitrophenylated chicken gamma-globulin, high dth responses were observed with lactobacillus casei and lactobacillus plantarum, but low responses with lactoba ... | 1996 | 8717402 |
| microbiology of mesu, a traditional fermented bamboo shoot product. | the use of mesu as a pickle and as the base of curry is a tradition in the darjeeling hills and sikkim of india. a total of 327 strains of lactic acid bacteria, representing lactobacillus plantarum, l. brevis and pediococcus pentosaceus were isolated from 30 samples of mesu. these species were present in all samples of raw bamboo shoots tested. mesu was dominated by l. plantarum followed by l. brevis; p. pentosaceus was isolated less frequently and recovered from only 40-50% of the mesu samples. ... | 1996 | 8722186 |
| [evaluation of biological fish silage in broiler chicken]. | biological fish silage was produced from the mixture of three low price fish species. after mixing it with molasse, papaya and pineapple wastes, and lactobacillus plantarum incoculation, the product was stored for seven days, dehydrated and added to several diets of formulated poultry feed as a substitute of fish meal. physical-chemical and microbiological analyses were done to the raw materials and final products. biological tests of acceptability indicated that chicken preferred diets up to 50 ... | 1995 | 8729265 |
| characterization of the locus responsible for the bacteriocin production in lactobacillus plantarum c11. | lactobacillus plantarum c11 secretes a small cationic peptide, plantaricin a, that serves as induction signal for bacteriocin production as well as transcription of plnabcd. the plnabcd operon encodes the plantaricin a precursor (plna) itself and determinants (plnbcd) for a signal transducing pathway. by northern (rna) and sequencing analyses, four new plantaricin a-induced operons were identified. all were highly activated in concert with plnabcd upon bacteriocin induction. two of these operons ... | 1996 | 8755874 |
| abnormal fecal flora in a patient with short bowel syndrome. an in vitro study on effect of ph on d-lactic acid production. | d-lactic acidosis associated with encephalopathy is a clinical condition that occurs in patients with short bowel syndrome. we studied the fecal flora and the composition of fecal water of a child who developed this unusual disorder. bacteriological studies showed that the patient's stool contained a marked predominance of gram-positive anaerobes. two strains were identified, lactobacillus plantarum and lactobacillus salivarius, as the main bacteria isolated. fecal water showed ph 4.8 and total ... | 1996 | 8769294 |
| a mannose-specific adherence mechanism in lactobacillus plantarum conferring binding to the human colonic cell line ht-29. | two lactobacillus plantarum strains of human intestinal origin, strains 299 (= dsm 6595) and 299v (= dsm 9843), have proved to be efficient colonizers of the human intestine under experimental conditions. these strains and 17 other l. plantarum strains were tested for the ability to adhere to cells of the human colonic cell line ht-29.l.plantarum 299 and 299v and nine other l. plantarum strains, including all six strains that belong to the same genetic subgroup as l. plantarum 299 and 299v, adhe ... | 1996 | 8779562 |
| [microbial silage production from eviscerated fish]. | microbial fish silage was produced from a mixture of several fish species that belong to the shrimp by-catch. they were mixed with molasses, fruits (pineapple and papaya), sorbate and a starter of lactobacillus plantarum atcc8014. process was evaluated by ph, acidity, consistency, exudate liquid, non-protein nitrogen, total volatile bases, microbial and toxicological tests. results indicated that acid production and ph reduction occurs during the first two days of processing, later these values ... | 1993 | 8779624 |
| production of hydroxy fatty acid (10-hydroxy-12(z)-octadecenoic acid) by lactobacillus plantarum from linoleic acid and its cardiac effects to guinea pig papillary muscles. | lactobacillus plantarum produced 10-hydroxy-12(z)-octadecenoic acid (10-ohoda) from linoleic acid. it was suspected that 10-ohoda might be closely related to leukotoxin (9,10-epoxy-12-octa-decenoic acid (ltx)) which was regarded as a toxic and/or defensive substance in living beings. the cardiac effect of 10-ohoda, which was biosynthesized by lactobacillus plantarum, on an isolated guinea-pig papillary muscle was determined. its effect on the contractile force of the tissue preparations was exam ... | 1996 | 8806645 |
| knockout of the two ldh genes has a major impact on peptidoglycan precursor synthesis in lactobacillus plantarum. | most bacteria synthesize muramyl-pentapeptide peptidoglycan precursors ending with a d-alanyl residue (e.g., udp-n-acetylmuramyl-l-ala-gamma-d-glu-l-lys-d-ala-d-ala). however, it was recently demonstrated that other types of precursors, notably d-lactate-ending molecules, could be synthesized by several lactic acid bacteria. this particular feature leads to vancomycin resistance. vancomycin is a glycopeptide antibiotic that blocks cell wall synthesis by the formation of a complex with the extrem ... | 1996 | 8808932 |
| a bacteriocin-like peptide induces bacteriocin synthesis in lactobacillus plantarum c11. | in this study, we show that bacteriocin production in lactobacillus plantarum c11 is an inducible process triggered by a secreted protein factor produced by the bacteriocin producer itself. the induction factor was identified to be plantaricin a, a bacteriocin-like peptide whose gene (plna) is located in the same operon as a two-component regulatory system (plnbcd). when l. plantarum c11 cultures were depleted for plantaricin a, either by growing individual colonies on agar plates or by starting ... | 1995 | 8817486 |
| utilization of fermented flocculated poultry sludge as a feed constituent for pigs. | flocculated poultry sludge was mixed with 3% molasses and was flow-therm pasteurized for 5 min at a core temperature of 95 c. the sludge was subsequently cooled to between 20 and 25 c and fermented with lactobacillus plantarum as starter culture. three groups of eight 8- to 10-wk-old, individually housed fattening pigs (hypor) were fed according to a fixed scheme correlated with age. one control group received a restricted ration of commercial compound feed (group a). the other control group was ... | 1995 | 8825585 |
| antibacterial activity of lactobacillus plantarum ug1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin ug1. | lactobacillus plantarum ug1 isolated from dry sausage produced an antimicrobial substance that inhibited other strains of the genera lactobacillus and lactococcus, and some foodborne pathogens including listeria monocytogenes, bacillus cereus, clostridium perfringens and clostridium sporogenes. this antibacterial substance was inactivated by proteolytic enzymes and showed a bactericidal mode of action. consequently, it was characterized as a bacteriocin, and was designated plantaricin ug1. this ... | 1996 | 8854175 |
| levels of antinutritional factors in pearl millet as affected by processing treatments and various types of fermentation. | pearl millet (pennisetum typhoideum) was fermented with lactobacilli or yeasts alone and in combination, and with natural microflora after various processing treatments, as grinding, soaking, debranning, dry heat treatment, autoclaving and germination. fermentation was carried out at 30 degrees c for 48 hours with lactobacillus plantarum (lp) and rhodotorula (r) isolated from naturally fermented pearl millet and lactobacillus acidophilus (la), candida utilis (cu) and natural microflora (nf). ger ... | 1996 | 8865334 |
| evaluation of numerical analysis of random amplified polymorphic dna (rapd)-pcr as a method to differentiate lactobacillus plantarum and lactobacillus pentosus. | lactobacillus plantarum and lactobacillus pentosus grouped into one protein profile cluster at r > or = 0.70, separate from lactobacillus casei, lactobacillus sake, and lactobacillus curvatus. similar sugar fermentation reactions were recorded for representative strains of l. plantarum and l. pentosus. representative strains, including the type of each species, were selected from the different protein profile clusters and their genetic relatedness determined by using numerical analysis of random ... | 1996 | 8867459 |
| biosynthesis of bacteriocins in lactic acid bacteria. | a large number of new bacteriocins in lactic acid bacteria (lab) has been characterized in recent years. most of the new bacteriocins belong to the class ii bacteriocins which are small (30-100 amino acids) heat- stable and commonly not post-translationally modified. while most bacteriocin producers synthesize only one bacteriocin, it has been shown that several lab produce multiple bacteriocins (2-3 bacteriocins). based on common features, some of the class ii bacteriocins can be divided into s ... | 1996 | 8879403 |
| lactic acid fermentation of cassava dough into agbelima. | the souring of cassava dough during fermentation into the fermented cassava meal, agbelima, was investigated. four different types of traditional inocula were used to ferment the dough and increases in titrable acidity expressed as lactic acid from 0.31-0.38 to 0.78-0.91% (w/w) confirmed the fermentation to be a process of acidification. the microflora of all inocula and fermenting dough contained high counts of lactic acid bacteria, 10(8)-10(9) cfu/g in all inocula and 10(7)-10(8), 10(8)-10(9) ... | 1996 | 8880299 |
| histamine and tyramine production by bacteria from meat products. | a series of 94 strains of lactic acid bacteria and micrococcaceae were tested for their ability to decarboxylate histidine and tyrosine in a laboratory medium. histamine and tyramine were quantified by using a fluorimetric and a hplc method. there was no significant difference between the results obtained with either method. among the strains tested, only three released histamine. on the other hand, all the strains of carnobacterium produced high concentrations of tyramine (2193 micrograms/ml). ... | 1996 | 8880339 |
| inhibition of microbial growth by ajoene, a sulfur-containing compound derived from garlic. | ajoene, a garlic-derived sulfur-containing compound that prevents platelet aggregation, exhibited broad-spectrum antimicrobial activity. growth of gram-positive bacteria, such as bacillus cereus, bacillus subtilis, mycobacterium smegmatis, and streptomyces griseus, was inhibited at 5 micrograms of ajoene per ml. staphylococcus aureus and lactobacillus plantarum also were inhibited below 20 micrograms of ajoene per ml. for gram-negative bacteria, such as escherichia coli, klebsiella pneumoniae, a ... | 1996 | 8900018 |
| the application of ph-sensitive fluorescent dyes in lactic acid bacteria reveals distinct extrusion systems for unmodified and conjugated dyes. | intracellular ph in bacteria can be measured efficiently between internal ph values of 6.5 and 8.5 with the fluorescent ph indicator 2',7'-bis-(2-carboxyethyl)-5[and-6]-carboxyfluorescein (bcecf). a new fluorescent ph probe with a lower pka(app) than bcecf was synthesized from fluorescein isothiocyanate and glutamate. the new probe, n-(fluorescein thio-ureanyl)-glutamate (ftug), was much less sensitive to changes in concentrations of kcl than was bcecf. similar to bcecf, an efflux of ftug indepe ... | 1996 | 8905646 |
| characterization of lactobacilli by southern-type hybridization with a lactobacillus plantarum pyrdfe probe. | lactobacillus plantarum, lactobacillus pentosus, and lactobacillus paraplantarum (m.-c. curk, j.-c. hubert, and f. bringel, int. j. syst. bacteriol. 46:595-598, 1996) can hardly be distinguished on the basis of their phenotypes. unlike l. plantarum and l. paraplantarum, l. pentosus ferments glycerol and xylose but not melezitose. we identified two l. pentosus strains (cnrz 1538 and cnrz 1544) which ferment glycerol and melezitose but not xylose. alpha-methyl-d-mannoside was fermented by 66% of t ... | 1996 | 8934911 |
| molecular cloning of manganese catalase from lactobacillus plantarum. | a genomic clone encoding manganese-containing catalase has been isolated from lactic acid bacterium lactobacillus plantarum, sequenced, and expressed in escherichia coli cells with an inducible expression system. the primary structure of the enzyme deduced from the nucleotide sequence, that comprises 266 amino acid residues, showed no significant homology with that of any other proteins registered on the available data bases. no peptide motifs conserved among active sites of proteins including m ... | 1996 | 8939876 |
| production of pediocin ach by lactobacillus plantarum whe 92 isolated from cheese. | among 1,962 bacterial isolates from a smear-surface soft cheese (munster cheese) screened for activity against listeria monocytogenes, six produced antilisterial compounds other than organic acids. the bacterial strain whe 92, which displayed the strongest antilisterial effect, was identified at the dna level as lactobacillus plantarum. the proteinaceous nature, narrow inhibitory spectrum, and bactericidal mode of action of the antilisterial compound produced by this bacterium suggested that it ... | 1996 | 8953710 |
| 13c nuclear magnetic resonance analysis of glucose and citrate end products in an ldhl-ldhd double-knockout strain of lactobacillus plantarum. | we have examined the metabolic consequences of knocking out the two ldh genes in lactobacillus plantarum using 13c nuclear magnetic resonance. unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain tf103 mainly produced acetoin. a variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol. | 1996 | 8955418 |
| the effect of lactobacilli inoculation on organic acid levels in the mucosal film and the small intestine contents in gnotobiotic pigs. | the effect of the inoculation of three lactobacillus plantarum strains upon lactic, acetic, acetoacetic and propionic acid levels in the mucosal film (f) and the jejunal and ileal contents (o) has been investigated in gnotobiotic pigs. in the jejunum of the inoculated animals, the mucosal film revealed significantly increased levels of lactic, propionic and acetoacetic acids when compared to the contents (25.3 vs. 10.8 mmol.l-1, 18.5 vs. 5 mmol.l-1 and 29.7 vs. 11.2 mmol.l-1, respectively) as we ... | 1996 | 8956538 |
| structure and organisation of the pyrimidine biosynthesis pathway genes in lactobacillus plantarum: a pcr strategy for sequencing without cloning. | this report describes the sequence and structural organisation of the pyrimidine biosynthesis pathway genes of lactobacillus plantarum ccm 1904. it also describes an in vitro technique based on pcr for sequencing without cloning. this new technique was developed because it was impossible to clone certain parts of the l. plantarum genomic dna in the escherichia coli host. l. plantarum pyr genes are organised as a 9.8-kb operon with the following order: pyrr, pyrb, pyrc, pyraa, pyrab, pyrd, pyrf a ... | 1996 | 8982065 |
| [production of biological silage from fish scraps]. | fish waste from the fish processing industry were used as a raw material to produce biological silage. the technology used had been previously developed and tested to optimize the process. the degree of grinding, molasses concentration, process temperature, lactobacillus plantarum inoculation, and utilization of tropical fruit wastes as a source of proteolytic enzymes were tested. results indicated that after process and storage for 90 days at room temperature, a stable product is obtained by us ... | 1994 | 8984967 |
| proteolytic activity of lactobacilli in a model goats' milk curd system. | mesophyllic lactobacilli cultures propagated in mrs broth were inoculated in goats' milk curd slurries and incubated at 30 degrees c for 10 d. the micro-organisms tested were lactobacillus casei subsp. casei ifpl 731 and ifpl 99, and lactobacillus plantarum ifpl 3. whole cells, cell-free extracts and cell lysates were evaluated for acceleration of proteolysis in the curd slurries. conversion of water-soluble nitrogen to non-protein nitrogen and amino acid nitrogen, reverse phase-hplc peak areas ... | 1996 | 8987897 |
| t cell receptor-alpha beta-deficient mice fail to develop colitis in the absence of a microbial environment. | mice with null mutations in cytokine or t cell receptor (tcr) genes develop intestinal inflammation. in the case of interleukin-2-/- and interleukin-10-/- mice it has been demonstrated that normal intestinal bacterial flora can cause gut pathology. tcr-alpha-/- mice not only develop colitis but also produce a strong antibody response to self-antigens, such as double-stranded dna. it is therefore important to establish whether the intestinal inflammation develops spontaneously or is induced by lu ... | 1997 | 9006326 |
| [change in various biological properties of lactobacillus as affected by ribonuclease]. | the influence of rnase from bacillus intermedius on the growth of the industrial strain lactobacillus plantarum 8r-a3 was studied. it was shown that the stimulating effect of the enzyme depended on its dose and manifested itself in decreasing the growth lag phase. at the same time the growth stimulating dose of rnase increased the lactobacillus adhesion to the epithelial cells and promoted secretion of proteinases from lactobacillus to the culture medium. the possible use of rnase as a stimulant ... | 1996 | 9027286 |
| effect of oral supplementation of lactobacilli on bacterial translocation in acute liver injury induced by d-galactosamine. | bacterial infections and sepsis are frequent complications of acute liver injury, with a high share in the mortality and morbidity of this condition. bacterial translocation from the gut may play an important role in the high rate of infections observed. in this experiment the effect of different oral supplementation on bacterial translocation was evaluated in acute liver injury induced by d-galactosamine in the rat. | 1997 | 9059965 |
| relative activity of n-(beta-d-glucopyranosyl)nicotinic acid to nicotinic acid as a niacin nutrient in rats and in lactobacillus plantarum atcc 8014. | we investigated the relative activity of n-(beta-d-glucopyranosyl)-nicotinic acid as a niacin nutrient in rats and in lactobacillus plantarum atcc 8014. n-(beta-d-glucopyranosyl)-nicotinic acid is a detoxified product or storage form of nicotinic acid that is found in plants. the relative activity of n-(beta-d-glucopyranosyl)nicotinic acid to nicotinic acid in rats was 1/2.3, 1/2.2, 1/1.0, and 1/1.7 as indices of the body weight gain, food intake, blood nad content, and the increased urinary exc ... | 1996 | 9063984 |
| plasmid integration in a wide range of bacteria mediated by the integrase of lactobacillus delbrueckii bacteriophage mv4. | bacteriophage mv4 is a temperate phage infecting lactobacillus delbrueckii subsp. bulgaricus. during lysogenization, the phage integrates its genome into the host chromosome at the 3' end of a trna(ser) gene through a site-specific recombination process (l. dupont et al., j. bacteriol., 177:586-595, 1995). a nonreplicative vector (pmc1) based on the mv4 integrative elements (attp site and integrase-coding int gene) is able to integrate into the chromosome of a wide range of bacterial hosts, incl ... | 1997 | 9068626 |
| high level heterologous protein production in lactococcus and lactobacillus using a new secretion system based on the lactobacillus brevis s-layer signals. | a secretion cassette, based on the expression and secretion signals of a s-layer protein (slpa) from lactobacillus brevis, was constructed. e. coli beta-lactamase (bla) was used as the reporter protein to determine the functionality of the s-layer signals for heterologous expression and secretion in lactococcus lactis, lactobacillus brevis, lactobacillus plantarum, lactobacillus gasseri and lactobacillus casei using a low-copy-number plasmid derived from pgk12. in all hosts tested, the bla gene ... | 1997 | 9074504 |
| processing and storage of lye-treated carrots fermented by a mixed starter culture. | a mixed culture of lactobacillus plantarum and saccharomyces cerevisiae was compared with a single culture of l. plantarum as starter for the fermentation of lye-treated carrots. using the mixed culture, more than 95% of glucose, fructose and malic acid was consumed after 7 days of fermentation in a brine containing 2.5% w/v nacl and 0.7% acetic acid, but only 54% of sucrose was degraded. the fermentation products quantified were lactic acid, ethanol and acetic acid and carbon recovery was 88%. ... | 1997 | 9081229 |
| arginine biosynthesis and regulation in lactobacillus plantarum: the cara gene and the argcjbdf cluster are divergently transcribed. | a cluster of citrulline biosynthetic genes has been cloned and sequenced from a fragment of lactobacillus plantarum ccm 1904 (atcc 8014) dna isolated as complementing a bacillus subtilis argf mutation. the gene order was cara-argcjbdf, with cara transcribed divergently from the arg cluster. although other gram-positive bacteria show similar arg clusters, this arrangement for cara is thus far unprecedented. downstream from the arg cluster, two open reading frames (orf7 and orf8) having unknown fu ... | 1997 | 9098069 |
| effect of bacterial galactosidase treatment on the nutritional status of soybean seeds and its milk derivative. | four field strains of lactobacillus plantarum (ls 4, 19, 21, 133) obtained from fufu (a semi-solid product obtained by boiling fermented cassava--manihot esculenta crantz) and a type strain dsm 2017 were grown on different carbon sources to induce galactosidase production. ls 21 produced the highest concentration of alpha- and beta-galactosidase with 0.28 mumol/l and 0.28 mumol/l respectively on lactose and galactose. milk obtained from soybean seeds treated with the enzyme mixture for 24 h show ... | 1997 | 9113667 |
| effects of the kinetics of water potential variation on bacteria viability. | the effect of the kinetics of water potential variation (psi) on the viability of bacteria subjected to hyperosmotic stresses in water-glycerol solution was studied. the three bacteria used were lactobacillus plantarum l-73, leuconostoc mesenteroides lm057 and escherichia coli tg1. these strains were submitted to a final water potential of -107.2 mpa, -170.9 mpa and/or -244.7 mpa. in any case the kinetics of water potential variation was found to have a great effect on the cell viability. the ap ... | 1997 | 9113880 |
| bacteriocins of lactobacillus plantarum strains from fermented foods. | bacteriocin-producing strains may be used as protective cultures to improve the microbial safety of foods. the crude or purified form of these antimicrobial agents may also be applied directly as food preservative. this review gives survey of the different bacteriocins produced by lactobacillus plantarum isolated from fermented food products with particular emphasis on their genetic and biochemical properties. a number of bacteriocins are produced by l. plantarum. these include plantaricin b, pl ... | 1996 | 9138308 |
| cloning and characterization of cspl and cspp, two cold-inducible genes from lactobacillus plantarum. | two cold shock genes, cspl and cspp, have been cloned from two lactobacillus plantarum strains. these genes, which are nonallelic, were present in all strains tested. the genes encode 66-amino-acid polypeptides related to each other and to the cold shock csp family. transcription of cspp rendered a single mrna, while two cspl mrnas were found with common 5' ends. the amounts of these transcripts increased moderately upon exposure of the cultures to cold. | 1997 | 9139925 |
| sequence analysis and characterization of pom1, a small cryptic plasmid from butyrivibrio fibrisolvens, and its use in construction of a new family of cloning vectors for butyrivibrios. | as a preliminary step in the development of vector systems, we have isolated and begun to characterize small, cryptic plasmids from several strains of the rumen bacterium butyrivibrio fibrisolvens. we present here the complete nucleotide sequence of butyrivibrio plasmid pom1, which was isolated from b. fibrisolvens bu49. while it is very similar in size to the previously characterized butyrivibrio plasmids prjf1 and prjf2, pom1 exhibits a restriction pattern which is quite distinct. analysis of ... | 1997 | 9143105 |
| molecular characterization of an inducible p-coumaric acid decarboxylase from lactobacillus plantarum: gene cloning, transcriptional analysis, overexpression in escherichia coli, purification, and characterization. | by using degenerate primers designed from the first 19 n-terminal amino acids of lactobacillus plantarum p-coumaric acid decarboxylase (pdc), a 56-bp fragment was amplified from l. plantarum in pcrs and used as a probe for screening an l. plantarum genomic bank. of the 2,880 clones in the genomic bank, one was isolated by colony hybridization and contained a 519-bp open reading frame (pdc gene) followed by a putative terminator structure. the pdc gene is expressed on a monocistronic transcriptio ... | 1997 | 9143125 |
| viability of cryptosporidium parvum during ensilage of perennial ryegrass. | the survival of cryptosporidium parvum during ensilage of perennial ryegrass was examined in laboratory silos with herbage prepared in one of three different ways; either untreated, inoculated with a strain of lactobacillus plantarum or by direct acidification with formic acid. the ph values of all silages initially fell below 4.5, but only formic acid-treated silage remained stable at less than ph 4 after 106 d, with the ph of the untreated and inoculant-treated silages rising to above 6. the f ... | 1997 | 9147867 |
| the alanine racemase gene is essential for growth of lactobacillus plantarum. | the lactobacillus plantarum alr gene encoding alanine racemase was cloned by complementation of an escherichia coli alr- dadx- double mutant strain. knockout of the alr gene abolished all measurable alanine racemase activity, and the mutant was shown to be strictly dependent on d-alanine for growth. | 1997 | 9171436 |
| use of virginiamycin to control the growth of lactic acid bacteria during alcohol fermentation. | the antibiotic virginiamycin was investigated for its effects on growth and lactic acid production by seven strains of lactobacilli during the alcoholic fermentation of wheat mash by yeast. the lowest concentration of virginiamycin tested (0.5 mg lactrol kg-1 mash), was effective against most of the lactic acid bacteria under study, but lactobacillus plantarum was not significantly inhibited at this concentration. the use of virginiamycin prevented or reduced potential yield losses of up to 11% ... | 1997 | 9172435 |
| 2-oxo-3-alkynoic acids, universal mechanism-based inactivators of thiamin diphosphate-dependent decarboxylases: synthesis and evidence for potent inactivation of the pyruvate dehydrogenase multienzyme complex. | a new class of compounds, the 2-oxo-3-alkynoic acids with a phenyl substituent at carbon 4 was reported by the authors as potent irreversible and mechanism-based inhibitors of the thiamin diphosphate- (thdp-) dependent enzyme pyruvate decarboxylase [chiu, c.-f., & jordan, f. (1994) j. org. chem. 59, 5763-5766]. the method has been successfully extended to the synthesis of the 4-, 5-, and 7-carbon aliphatic members of this family of compounds. these three compounds were then tested on three thdp- ... | 1997 | 9201955 |
| dna probe and pcr-specific reaction for lactobacillus plantarum. | a 300 bp dna fragment of lactobacillus plantarum isolated by randomly amplified polymorphic dna (rapd) analysis was cloned and sequenced. this fragment was tested using a dot-blot dna hybridization to technique for its ability to identify lact. plantarum strains. this probe hybridized with all lact. plantarum strains tested and with some strains of lact. pentosus, albeit more weakly. two internal primers of this probe were selected (lbp11 and lbp12) and polymerase chain reaction (pcr) was carrie ... | 1997 | 9202445 |
| characterization and selection of lactobacilli isolated from spanish fermented sausages. | the use of starter cultures to control and run the fermentative process is a usual way of manufacturing sausages in meat industries. the first stage in the starter culture designing process is to characterize the lactic acid bacteria isolated from these meat products, in order to select the best strains. the strains used for this study were isolated from different dry fermented sausages, obtained during the manufacturing process. the main tests used to identify the isolated bacteria were: micros ... | 1997 | 9253760 |