| [further experimental investigations on the therapy of gas gangrene with ozone and oxygen (author's transl)]. | in continuation of a recently-published investigation on the effect of intramuscular injections of an ozone-oxygen mixture on experimental gas gangrene in the guinea pig, 2 additional experiments were performed in order to establish the efficacy of the therapy when given twice daily instead of only once. futhermore, the effect of an analogous therapy was investigated with the use of pure oxygen. the ozone-oxygen therapy reduced the death rate from 100% in untreated control animals to 73.6 and 70 ... | 1976 | 183374 |
| vancomycin. | | 1976 | 183412 |
| [gas formation during cultivation of cl. perfringens]. | | 1976 | 183427 |
| fluorimetric assay of sialic acids. | | 1976 | 183563 |
| phospholipase c assay using p-nitrophenylphosphoryl-choline together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. | | 1976 | 183567 |
| effect of clostridium toxoids, especially of clostridium perfringens toxoid, on mouse transplanted tumors. | the antitumor activities of toxoids of clostridium perfringens, c. nouyi, c. septicum, and c. tetani were tested against sarcoma-180 and ehrlich carcinoma. among them, c. perfringens toxoid showed a high antitumor activity against the growth of the implanted sarcoma-180 ascites form. the results of the inhibiting effect of c. perfringens toxoid on nakahara-tokuzen-fukuoka (ntf) reticulum cell sarcoma and methylcholanthrene-induced fibrosarcoma were also described. | 1976 | 184006 |
| pathologenesis of hematogenous bacterial meningitis in rabbits. | the authors present data obtained from a series of 27 rabbits studied following intracarotid injection of saline, brain-heart infusion broth, aerobic, or anaerobic bacteria. these data support the hypothesis that injections of cultures of both aerobic and anaerobic organisms by way of the carotid artery disrupts the blood-brain barrier over the cerebral convexities within 15 minutes; however, the inflammatory response and bacterial proliferation occur much more rapidly in the ventricular system. ... | 1976 | 184258 |
| [effect of the products of the vital activity of clostridium aerofoetidum on the hemolytic and lethal properties of the causative agents of anaerobic gas infection]. | | 1976 | 184368 |
| laboratory diagnosis of foodborne diseases. | many bacterial species are responsible for sporadic cases and outbreaks of foodborne intoxication and infection. the foodborne diseases are classified on the basis of the pathogenetic mechanisms involved into four categories: performed toxin, enterotoxin formed in the colonized small intestine, mucosal invasion (enterocolitis) and mucosal invasion with bacteremia. invasive and toxigenic strains of enteropathogenic escherichia coli are discussed. in vivo test systems for the identification of ent ... | 1976 | 184732 |
| lecithinase-negative variants of clostridium perfringens; the identity of c. plagarum with c. perfringens. | identity of clostridium plagarum (prévot) com. nov. (1938) with c. perfringens was demonstrated in the tests for the cultural and biochemical properties, dna-dna homology, susceptibility to c. perfringens-specific lysin, complementary synthesis of c. perfringens theta-toxin between c. plagarum and theta-toxin-negative mutants of c. perfringens, as well as the tests for gas-chromatographic patterns. | 1976 | 184898 |
| anomalous aggregation of clostridium perfringens enterotoxin under dissociating conditions. | polyacrylamide gel electrophoresis of highly purified clostridium perfringens enterotoxin revealed electrophoretic microheterogeneity of the enterotoxin, apparently because of slight charge differences in the peptides. detergent gel electrophoresis showed that purified enterotoxin formed high molecular weight aggregates in the presence of both sodium dodecyl sulfate (sds) and cetyltrimethylammonium bromide. no conditions capable of inhibiting this phenomenon were found. although a molecular weig ... | 1976 | 184907 |
| acute intravascular hemolysis with minimal renal impairment in clostridium perfringens infection. | we report here a case of post-abortal clostridium prefringens infection in which there was severe intravascular hemolysis with black urine, but only minor abnormalities of the clotting mechanism and mild renal failure. the patient recovered following supportive therapy only. | 1976 | 184994 |
| clostridial cellulitis with localized gas formation in a dog. | | 1976 | 185182 |
| the skin flora of the hemiplegic hand. | more organisms were found on the paralysed hands of hemiplegic patients than on their unaffected ones. however, both showed considerably higher bacterial counts than did geriatric patients without paralysis. an infection rate for cl. welchi of 15% was found in the paralysed hands. | 1976 | 185286 |
| specific antibody against clostridium perfringens type a enterotoxin in chinese on taiwan. | | 1976 | 185320 |
| clostridial spontaneous peritonitis. | | 1976 | 185433 |
| [bacteria from the genus of clostridium as causative agents of food poisoning in man (author's transl)]. | | 1976 | 185478 |
| effect of nisin on heat resistance and gas production of some spore-formers. | | 1976 | 185844 |
| beneficial effect of catalase treatment on growth of clostridium perfringens. | several common plating media were tested for their ability to support growth of clostridium perfringens after storage of the plates for 1 to 10 days at 4 and 25 degrees c with and without subsequent addition of catalase. liver-veal (lv) agar and brain heart infusion (bhi) agar quickly become incapable of supporting growth after storage without added catalase, whereas shahidi ferguson perfringens (sfp) agar and brewer anaerobic (ba) agar were less affected. plate counts of c. perfringens on untre ... | 1976 | 185958 |
| clostridium perfringens infection in turkey poults. | necropsy of dead and sick 5-day-old poults from a flock of 12,500 broad-breasted white turkeys having high early mortality (685 the first week) revealed rather large inflamed retained yolk sacs with semiliquid contents, ascites, and swollen greenish-colored livers. cultures of livers and yolk sacs were negative by standard aerobic procedures for salmonella and any other bacterial pathogens. at the beginning of the 4th week, more poults were necropsied and found to have swollen livers with necrot ... | 1976 | 186016 |
| persistent high numbers of clostridium perfringens in the intestines of japanese aged adults. | tsn agar was applicable for enumeration of clostridium perfringens in fecal samples of adults but not in those of infants. it was demonstrated using tsn agar that some healthy aged adults had persistently carried c. perfringens at levels ranging from 10(7) to 10(9), while some others ranged from 10(3) to 10(6) per ml volume of fecal sample although all of these adults had the same diets. in the test for agglutinability of isolates of c. perfringens collected from two elderly adults, a younger ad ... | 1976 | 186657 |
| conformations of a,t-rich dnas. | dnas from the genomes of clostridium perfringens and cytophaga johnsonii display orthodox a-dna and b-dna structures despite their high (a+l) nucleotide content. unique structures, such as those found for synthetic dnas having specific special sequences, do therefore not necessarily occur for dnas having more random base sequence even if these have unusual base compositions. clostridium perfringens dna exhibits unusual structural properties only prior to purification by gel filtration. | 1976 | 186759 |
| [disease of newborn piglets caused by c1. perfringens]. | | 1976 | 186937 |
| an enterotoxin produced by clostridium perfringens type d. purification by affinity chromatography. | clostridium perfringens type d 9867 produced enterotoxin immunologically identical to that produced by types a and c which are responsible for c. perfringens food poisoning. enterotoxin from type c 5386 and type d 9867 was produced in duncan og strong sporulation medium (ds medium). the supernatant fluid from ds-cultures was used for purification of the enterotoxin by affinity chromatography on a monospecific anti-enterotoxin-coupled cnbr activated-sepharose 4b and activated ch-sepharose 4b colu ... | 1976 | 187011 |
| effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in clostridium perfringens. | activities of glyceraldehyde-3-phosphate dehydrogenase (ec 1.2.1.12) (gap-dh) and aldolase (ec 4.1.2.13) in cells of clostridium perfringens that had been inhibited with sodium nitrite were investigated. a complete loss in gap-dh activity and a 67% decrease in aldolase activity were observed when growth of c. perfringens was inhibited. there was also a 91% decrease in the concentration of free sulfhydryl groups of soluble cellular components. dithiothreitol restored some activity to inactive gap ... | 1976 | 187114 |
| [presence and pathogenic significance of clostridium perfringens type a in swine]. | | 1976 | 187166 |
| necrotic enteritis in broiler chickens. iii. reproduction of the disease. | lesions typical of necrotic enteritis could be produced experimentally in from 11-26% of broiler chickens consuming feed containing approximately 10(7) clostridium perfringens per gram. highest mortality was produced using isolates from field cases of necrotic enteritis which were reisolated from experimental cases in the laboratory. penicillin in the drinking water at 100,000 i.u./litre completely prevented mortality whereas chloramphenicol at 110 mg/litre delayed the onset and reduced the numb ... | 1976 | 187291 |
| the use of a multicomponent standard for clostridial vaccines. | a multicomponent clostridial vaccine in being developed with the objective of acting as a standard preparation for the control of vaccines containing one to eight components. it is intended initially for use in monitoring those components which can be assessed by antitoxin response assays in rabbits. the components involved, therefore, are the principle toxoids of clostridium (perfringens) welchii types b, c and d, cl. oedematiens type b, cl. septicum and cl. tetani. the proposed standard also c ... | 1976 | 187507 |
| an international survey of clostridial sera and vaccines. | the papers present the results of a survey of the usage, assay and specification of veterinary clostridial sera and vaccines in 23 countries. thirteen of the countries use up to 8 different antisera. all the countries use vaccines, which are prepared from 13 species and types of clostridia. vaccines containing up to 8 such components are commonly employed. criteria for the design and interpretation of assays are discussed and evidence for efficacy summarized. | 1976 | 187508 |
| production and standardization of polyvalent clostridium perfringens vaccine in iran. | in this presentation an account of the large-scale production and the standardization of polyvalent clostridium perfringens vaccine in iran is described. over 20 million doses of this vaccine are produced and utilized in iran every year. certain modifications have been made to the culture media used in our laboratory for the production of this vaccine in order to bring down its cost. the prepared vaccine is highly immunogenic as determined by the laboratory examination on the quality of the vacc ... | 1976 | 187509 |
| the standardization of cl. perfringens antigens and antisera. | the preparation of laboratory standard antitoxins against cl. perfringens beta and epsilon toxins is described. these antitoxins are suitable for the quantitative determination of the corresponding antigens by means of the flocculation test. the flocculation test was, however, shown to be more suitable for determining the antigenic value of fresh toxoid rather than toxoid stored without neutralization of excess formalin. a maximal immunity response to alum-precipitated epsilon toxoid was obtaine ... | 1976 | 187510 |
| [standardization of the control technics of clostridium perfringens vaccine. use of a reference vaccine]. | control of cl perfringens toxins. it is possible to employ a standardized technique for the control of the toxins of cl. perfringens. titres of ld50 per ml for the mouse and l+/50 per ml may be expressed with a confidence limit of 1.5. the assay of toxins by l+/50 (relating to their antigenic value) permits the establishment of standards by which it is possible to obtain a satisfactory vaccine with a high degree of certainty. 10 l+/50 per dose for cl. perfringens a 20 l+/50 per dose for cl. perf ... | 1976 | 187511 |
| [comparison between the official indirect method and the direct method of control of vaccines against sheep enterotoxemia]. | the official methods of control of vaccines against sheep enterotoxemia are indirect in two ways : on the one hand they are carried out on animals for which the vaccine is not intended, and on the other hand they test only an immunological serum reaction. another method consisting of direct testing by intravenous injection of toxin has been carried out on mice previously vaccinated with different doses. it has been shown that it is possible to obtain a certain level of protection giving a good d ... | 1976 | 187512 |
| experimental studies on serum treatment and vaccination against cl. perfringens type c infection in piglets. | subcutaneous administration of serum to piglets just after birth resulted in serum titres of 9-18 i.u. beta-antitoxin per ml in the first three days of life. at the ages of 7, 14, and 28 days the titres had dropped to about 5-9, 2 and 1 i.u. per ml, respectively. oral administration of the same dose of serum resulted in serum titres of about half of those found after s.c. administration. in infected herds a significant protective effect after both s.c. and oral administration of serum was found ... | 1976 | 187513 |
| solid-phase radioimmunoassays for quantitative antibody determination of bacterial exotoxins. measurement of clostridium perfringens type d epsilon antitoxin. | two reversed solid-phase radioimmunoassays have been developed for quantitative determination of antibodies against clostridium perfringens type d epsilon toxin. 125i labelled prototoxin was used in the bromoacetyl cellulose-bound antibody method and in the antibody coated tube method. the procedures are based on the competition for 125i labelled prototoxin between the insoluble antibodies and the antibodies present in the unknown sample. the radioactivity bound to solid-phase is in inverse rati ... | 1976 | 187514 |
| an international serotyping system for clostridium perfringens (welchii) type a in the near future. | the importance of clostridium perfringens (welchii) type a as an agent of clinical infection in man is well known and, more recently, its role as a cause of human food poisoning has become well recognized. there has been a growing awareness of the need for a system of finer subdivision within the type a group for better understanding of the epidemiology of cl. perfringens in infections and food poisoning. a set of antisera used for typing and developed in the food hygiene laboratory over a perio ... | 1976 | 187515 |
| quality control for fermented meats. | | 1976 | 187566 |
| characterization of one neutral and two acidic glycoasparagines isolated from the urine of patients with aspartylglycosylaminuria (agu). | one neutral and two acidic glycoasparagines were isolated from the urine of patients with aspartylglycosylaminuria (agu). the neutral one was identified as beta-gal-(1 leads to 4)-beta-glcnac-asn. the acidic ones were composed of 1 mole of sialic acid and 2 moles each of galactose and n-acetylglucosamine, attached to asparagine, and were isomeric with respect to the position of sialic acid attachment since they produced the same glycoasparagine on incubation with the neuraminidase [ec 3.2.1.18] ... | 1976 | 187576 |
| gonadotropin receptors in plasma membranes of bovine corpus luteum. i. effect of phospholipases on the binding of 125i-choriogonadotropin by membrane-associated and solubilized receptors. | the ability of bovine corpus luteum plasma membranes to bind 125i-choriogonadotropin has been examined after prior treatment of the membranes with phospholipases a, c, and d. treatment of the purified membranes with low concentrations of phospholipases a and c resulted in the inhibition of the binding of 125i-choriogonadotropin to its receptors, whereas phospholipase d had no effect. receptor activity was decreased by low concentrations of phospholipase a from either bee venom, vipera russelli o ... | 1976 | 187585 |
| improved rubella hemagglutination inhibtion test: inactivation of non-immunoglobulin hemagglutination inhibitors by phospholipase c. | a method using phospholipase c (pl-c) for removing nonspecific inhibitors (nsi) of rubella virus hemagglutinin is described. pl-c was found to hydrolyze nsi without altering the hemagglutination inhibition (hi) activity of the specific antibody and could be used to remove nsi in the rubella hi test by using formalinized erythrocytes, which resisted the enzymatic action; fresh erythrocytes were lysed by pl-c. the hi test using pl-c treated sera gave true measurements of actual rubella antibody co ... | 1976 | 187617 |
| [dynamics of clostridium perfringens type a no.28 and staphylococcus 0-15 interaction]. | | 1976 | 187906 |
| synergistic bacterial gangrene following abdominal hysterectomy. | a case of synergistic bacterial gangrene occurring after abdominal hysterectomy in a 43-year-old patient is presented. after multiple debridement procedures and porcine grafts, the patient responded satisfactorily and eventually recovered. | 1977 | 187992 |
| [clostridium perfringens infection. problems posed excluding resuscitation]. | the authors report 18 cases of clostridium perfringens infection and discuss the main clinical and etiological aspects apart from gynecological forms and those usually requiring intensive care. pathological foci, digestive infection and the problem of bacteremia are discussed together with appropriate treatment. | 1976 | 188174 |
| [the interspecies masking between staphylococcus pyogenes and clostridium perfringens toxins]. | | 1976 | 188273 |
| [study of leukotoxic activity of cl. perfringens type a toxin and its fractions obtained by gel filtration and ion exchange chromatography]. | in gel-filtration of cl. perfringens type a toxin on sephadex f-100 and f-50 there was revealed relationship between the leukotoxic activity and a high-molecular component. a method of ion-exchange chromatography on a column with deae-sephadex a-25 from the cl. perfringens toxin there were obtained 8 fractions 3 of which possessed a marked leukotoxic activity: the capacity to destroy neutrophils in the svejcar and vancurik test and to-depress the phagocytic activity of leukocytes. lecithinase an ... | 1976 | 188277 |
| a progressive approach to the problem of foodborne infections. | qualitative and quantitative bacteriological examinations of 100 samples of perishable foods from 39 retail stores were performed to determine the presence of bacterial contaminants and to explore the feasibility of establishing and utilizing microbiological standards in enforcement. forty-six per cent of the samples had standard plate counts in excess of 100,000 per gram, 17 per cent showed coliform organisms in excess of 100 per gram, 20 per cent revealed the presence of staphylococcus aureus ... | 1977 | 188346 |
| "phoenix phenomenon" in the growth of clostridium perfringens. | the "phoenix phenomenon" was observed with clostridium perfringens hobbs' serological type 9 (ht9) in a cooked-meat medium at 81.7 degrees c by a decrease in plate count (phase i), followed by an increase in count to the intiial level (phase ii) and a continued increase above the initial count (phase iii). the effects of sporulation, age of inoculum, assay medium, anaerobiosis, diluent, and growth inhibitor were studied. this phenomenon was reproduced in experiments with sporulation-negative mut ... | 1976 | 188384 |
| [necrotic enteritis in broiler chickens. iii. study of the factors favoring the multiplication of clostridium perfringens and the experimental transmission of the disease]. | necrotic enteritis was reproduced experimentally in two week old broiler chickens by intravenous injection and also by oral administration of a pure culture of clostridium perfringens. in the first experiment, gross and microscopic intestinal lesions, typical of necrotic enteritis, were observed in all diseased birds and mortality was obtained only in the group of birds that were injected with 0.4 ml or more of the pure culture of the microorganism. in the second experiment, the highest mortalit ... | 1977 | 188529 |
| [ultrastructure of micropredators of gram-positive bacilli and the degeneration of c perfringens]. | | 1976 | 188562 |
| stable l-forms of clostridium perfringens: growth, toxin production, and pathogenicity. | growth and toxin production of stable l-forms of clostridium perfringens grown in a mini-fermentor were monitored. a gradual but steady increment in viable count occurred over a 7-h period, followed by death. the peak of viability preceded the optical density peak by 3 h. theta, alpha, kappa, and lambda toxins were measured, with theta toxin appearing first in the culture supernate. growth of the parent bacillus form of c. perfringens was compared under similar conditions. toxin levels achieved ... | 1977 | 188763 |
| laboratory confirmation of an outbreak of clostridium perfringens food poisoning. | a foodborne outbreak in a group of eleven individuals sharing a common meal, eight of whom reported symptoms and an incubation period compatible with clostridium perfringens food poisoning, was confirmed by the following laboratory criteria: (a) the c. perfringens count in the suspect food, chicken pie, was at least 10(5) per gram; (b) the median fecal c. perfringens spore count in convalescing patients was greater than 10(6) per gram: (c) an indentical serotype (ps63) was isolated from both the ... | 1977 | 188789 |
| bacteriological quality of ingredients used in ground beef manufacture. | | 1977 | 188798 |
| [properties of ionic forms of spores of a clostridium perfringens]. | | 1976 | 189102 |
| in vitro aggregation of platelets induced by alpha-toxin (phospholipase c) of clostridium perfringens. | highly purified alpha-toxin (phospholipase c) of clostridium perfringens prepared by affinity chromatography on agarose-linked egg-yolk lipoprotein induced the in vitro aggregation of platelets of an irreversible type. the aggregation started after a time lag, the length of which depended on the concentration of the toxin; the reciprocal of the time lag was found to be directly proportional to the toxin concentration. using this assay method, we demonstrated that the platelet-aggregating activit ... | 1976 | 189107 |
| [immunologic indicators in patients with suppurative processes in the abdominal cavity]. | | 1976 | 189120 |
| parasitic nematode larvae as agents of transmission of pathogenic bacteria. | | 1976 | 189513 |
| [use of a jet injector for administration of sorbed typhoid vaccine with polytoxoid]. | | 1976 | 189534 |
| [researches on the water quality of the river po and its tributaries between cremona and casalmaggiore. i. materials and methods (author's transl)]. | during 1971 a research program about the status of pollution of the river po and its tributaries was started by six institutes of hygiene altogether (turin, pavia, milan, parma, modena and ferrara universities) with the institute for water research of c.n.r. and hydrographic office of magistracy of the po. the role of the parma university hygiene institute was to study the water qualities of the river po between cremona and casalmaggiore and of two right side tributaries, arda-ongina and taro. t ... | 1976 | 189776 |
| characterization of dna strand breakage in vitro by the antitumor protein neocarzinostatin. | the antitumor protein antibiotic neocarzinostatin causes strand scission of dna in vitro in the presence of a sulfhydryl compound. the breaks are single stranded in nature and bear 5'-phosphoryl termini. all four deoxymononucleotides are recoverable at the 5'-ends of the cleavage sites although a higher proportion of dgmp and tmp are consistently found. the lesions are not repairable with polynucleotide ligase from escherichia coli. a quantitative assay was developed to determine the ph profile ... | 1977 | 189801 |
| [changes in the electrical activity of the cerebral cortex under the combined influence of cl. perfringens type a toxin and products of cl. butyricum activity]. | a study of the changes of the electrocorticogram (ecog) and the electrocardiogram (ecg) in combined action of cl. perfringens, type a, and of the cl. butyricum broth culture filtrate showed that desynchronization of the cortical electrical activity and its subsequent depression occurred at earlier periods than in the case of isolated administration of cl. perfringens toxin. the general character of the changes in the cortical rhythmic activity remained the same as in intoxication caused by cl. b ... | 1976 | 189855 |
| erythromycin-resistant clostridium welchii. | | 1977 | 189875 |
| interaction of pt(ii) complexes with dnas from various sources. a circular dichroism study. | the interaction of cis- and trans-pt(nh3)2cl2 with dnas of different base composition was studied by means of circular dichroism (cd) spectroscopy. the spectra obtained indicated a partial transformation of the secondary structure of dna (b- less than c transition) with an increased helix winding. subtle differences were noted between the two isomers, possibly related to changes in base orientation due to the different molecular geometries of the p+(ii) complexes. | 1977 | 189945 |
| [lipase activity of anaerobic bacteria on glycerol-tributyrate determined by gas-liquid chromatography]. | anaerobic bacteria are classified among other criteria by the presence or absence of phospholipase and lipase. the liquid gas chromatographic method detects with great sensibility the lipasic activity of the anaerobic bacteria. a liidolytic action has been demonstrated in cl. perfringens. | 1977 | 189951 |
| [sanitary studies on the control of a sterilizaton process in a rendering plant. 1. sanitary studies on a rendering plant utilizing the anderson process]. | | 1977 | 189983 |
| evidence for stable messenger ribonucleic acid during sporulation and enterotoxin synthesis by clostridium perfringens type a. | stable messenger ribonucleic acid (mrna) was shown to be involved in both enterotoxin synthesis and synthesis of other spore coat proteins in clostridium perfringens. when used at a concentration that inhibited [14c]uracil incorporation, rifampin, a specific inhibitor of deoxyribonucleic acid-dependent rna polymerase, prevented incorporation of a mixture of labeled amnoo acids by 3-h sporulating cells. at that time, enterotoxin protein was first detectable and cells were primarily at stage ii or ... | 1977 | 190209 |
| the possible welchihin activity of clostridium perfringens type a enterotoxin. | | 1976 | 190663 |
| vascular permeability increase by alpha-toxin (phospholipase c) of clostridium perfringens. | | 1977 | 190737 |
| clostridial myositis in cattle: bacteriology and gross pathology. | in a bacteriological study of 173 cases of clostridial myositis in cattle, cl chauvoei either alone or with cl septicum was demonstrated in 97 (56 %)min a further 62 (36%) cases cl novyi was demonstrated either alone or with cl septicum. on 11 (6%) occasions cl septicum only was recovered, and on another three 1-7%) cl sordelli only. the gross pathology of the lesions was correlated with the bacteriological findings, and prophylaxis in relation to the infections identified is discussed. | 1977 | 190758 |
| [presence and pathogenic significance of clostridium perfringens in the calf. 1. quantitative bacteriologic studies on the clostridium perfringens count in feces and intestinal contents of healthy and diseased calves]. | | 1977 | 190826 |
| [purification of the toxoids of cl. perfringens and cl. pedematiens using silicagel]. | a possibility was shown of purification of the concentrated toxoids of the cl. perfringens and cl. oedematiens species in examination of ksk-2, ksk-2,5; mca-2 silicagels. the specific activity of the preparations obtained and the yield approached the analogous indices obtained in stratification of sephadex g-75. the effect of preliminary silicagel treatment and also of the nature of buffer solutions on the efficacy of the process of purification of the gangrenous toxoids was established. | 1977 | 190831 |
| chemical modification and characterization of enterotoxin from clostridium perfringens type a. | enterotoxin from clostridium perfringens type a has been purified. the enterotoxin was shown to be heat-labile, but re-activation of heat treated enterotoxin did occur down to an activity of 15 per cent of the native enterotoxin. the molecular weight was shown to be 34,000 by ultracentrifugation, and the molecular weight did not change significantly after treatment with 0.1 m beta-mercaptoethanol and 6 m guanidine hydrochloride. it was concluded that the enterotoxin consists of one single polype ... | 1977 | 190854 |
| role of anaerobic bacteria in spontaneous peritonitis of cirrhosis: report of two cases and review of the literature. | the role of anaerobic or microaerophillic bacteria in spontaneous peritonitis of cirrhosis has not been clearly defined. among 126 cases recorded in the literature, in only eight (6 per cent), including the two reported here, was bacterascites associated with anaerobic or microaerophilic bacteria. clinical features in these cases were indistinguishable from those associated with aerobic bacteria. however, polymicrobial bacterascites occurred in four of eight cases associated with anaerobes, as c ... | 1977 | 190883 |
| [identification of two plasmids isolated from a bacteriocinogenic strain of clostridium perfringens]. | two plasmids were found and studied in the bacteriocinogenic strain n5 of clostridium perfringens: one is a bacteriocinogenic factor (mw=5.7 x 10(6)) and the other a cryptic plasmid (mw 32.4 x 10(6)). simultaneous loss of the ability to produce bacteriocin and of the bacteriocin resistance in a "cured" variant corresponds to the loss of the bacteriocinogenic plasmid dna. the syntheses of haemolysin 0 and phospholipase c do not seem to be coded for by the cryptic plasmid. | 1976 | 190933 |
| [experimental reproduction of necrotic enteritis in the chicken. 1. mono- and polyinfections with clostridium perfringens and coccidia with reference to cage managemen]. | experimental mono-infection and poly-infection, using vegetative germs of a cl. perfringens type a strain 1663 and its toxin as well as e. acervulina, necatrix or mitis oocysts, were applied to 100 spf chicken aged seven days. while clostridial or coccidial mono-infection did not cause any loss and only unspecific pathologic-anatomic changes, polyinfection was accompanied by diarrhoea and slower weight increase, with 55.4 per cent of the chicken having been lost three to nine days from the outbr ... | 1976 | 190968 |
| [experimental reproduction of necrotic enteritis in the chicken. 2. further mono- and polyinfections with cl. perfringens and coccidia with special reference to ground-kept chickens]. | coccidial oocysts and/or vegetative germs, spores, and toxins of cl. perfringens type a were used in mono-infection and poly-infection experiments on spf chicken aged seven and 56 days and kept under different conditions. necrotic enteritis was regularly reproduced in all experimental groups with polyinfections. in chicken aged seven days necrotic enteritis was reproduced even after repeated mono-infections with 4 x 10(9) gerus and toxin of cl. perfringens. the loss figures recorded from ground- ... | 1976 | 190969 |
| effects of concanavalin a and neuraminidase on cyclic amp levels and 14c-1-glucose oxidation in dog thyroid slices. | treatment with concanavalin a at 100 micron/ml or higher concentrations significantly increased 14c-1-glucose oxidation in dog thyroid slices as reported in other tissues. this treatment exerted no effect on tissue cyclic amp levels. neuraminidase at the same concentrations also had similar effects on these parameters. neither concanavalin a nor neuraminidase at the concentrations up to 100 microng/ml had the tsh effect on both tissue cyclic amp and 14c-1-glucose oxidation. these results indicat ... | 1976 | 191247 |
| effect of neuraminidase on the chromatographic behaviour of eleven acid hydrolases from human liver and plasma. | 1. the elution profiles of eleven acid hydrolases from human liver and plasma were directly compared using a system whereby a single salt gradient was simultaneously applied to two deae-cellulose chromatographic columns. 2. plasma alpha-l-fucosidase, alpha-mannosidase, alpha-galactosidase and alpha-glucosidase isoenzymes were eluted at higher salt concentrations than the corresponding liver isoenzymes whereasbeta-n-acetylglucosaminidase, beta-galactosidase, beta-glucosidase, exo-1,4-beta-xylosid ... | 1977 | 191258 |
| purification and some properties of phospholipase c (alpha-toxin) of clostridium perfringens. | 1. phospholipase c[ec 3.1.4.3] was purified from the culture filtrate of clostridium perfringens by successive chromatographies on cm-sephadex, deae-sephadex, and sephadex g-100. during the purification it was noted that, beside the monomer form of the enzyme which was purified, a part of the enzyme existed in active polymerized forms. 2. the purified preparation gave a single band on polyacrylamide gel electrophoresis and gave a single precipitin line in immunodiffusion with the national standa ... | 1977 | 191435 |
| direct assignment of the cysteinyl, the slowly exchangeable, and the aromatic ring 1h nuclear magnetic resonances in clostridial-type ferredoxins. | we have directly assigned the 1h nmr corresponding to the cysteinyl protons, the slowly exchangeable protons, and the aromatic ring protons in the 1h nmr spectrum of clostridium acidi-urici ferredoxin by isotopic labeling and 13c nmr decoupling techniques. we also show that the resonance pattern in the 8- to 20-ppm (from 2,2-dimethyl-2-sialapentanesulfonic acid) region of the 1h nmr spectra of oxidized clostridium acidi-urici, clostridium pasteurianum, clostridium perfringens, and peptococcus ae ... | 1977 | 191457 |
| [characteristics of the proteolytic complex of clostridium perfringens type a]. | | 1976 | 191802 |
| [ovine enterotoxemia caused by cl. perfringens type a]. | | 1976 | 191981 |
| [experience in treating sheep with clostridial infections]. | | 1976 | 191982 |
| [purification and some properties of "clostridium perfringens" delta toxin (author's transl)]. | delta toxin, a hemolytic exocellular protein excreted by c. perfringens type c has been purified to homogeneity, assessed by polyacrylamide disc gel electrophoresis. purification steps involved successively calcium phosphate gel formation in culture supernatant fluid, salting-out of unadsorbed material by ammonium sulfate to 50 % saturation, isoelectric focusing and gel filtration on sephadex g75. purified toxin appears as a basic protein occuring in two forms with isoelectric points of 8.8 and ... | 1976 | 192118 |
| amorphous ferrous sulfide as a reducing agent for culture of anaerobes. | amorphous ferrous sulfide, prepared by reacting ferrous ammonium sulfate and sodium sulfide, is an excellent reducing agent for the culture of anaerobes. it reduces resazurin and reacts much more rapidly with o2 than does either soluble sulfide (hs)- or cysteine. one of the end products of the oxidation of ferrous sulfide with o2 is red and serves as an indicator for the oxygen contamination of a culture medium. amorphous ferrous sulfide served as a suitable reducing agent for the growth of spec ... | 1977 | 192144 |
| capsular polysaccharide of clostridium perfringens hobbs 9. | several closely related capsular polysaccharides were isolated from a strain of clostridium perfringens hobbs 9 type a by extraction of encapsulated cells with cold 0.85% nacl. the soluble polymers were precipitated with alcohol and purified by (nh4)2so4 fractionation, enzymatic digestion with papain and ribonuclease, and chromatography on diethylaminoethyl-sephadex a25. the polysaccharides were composed mainly of glucose, galactose, and galactosamine. the major fraction contained these constitu ... | 1977 | 192674 |
| effect of clostridium perfrongens enterotoxin in mitochondrial respiration. | clostridium perfringens enterotoxin caused a 26 to 41% reduction in the rate of oxygen consumption by rat liver mitochondria when various tricarboxylic acid cycle intermediates were used as substrate. however, p/o ratios were unaltered. | 1977 | 192680 |
| anaerobic bacteria in biliary disease in elderly patients. | gallbladder bile from 52 elderly subjects who had undergone biliary tract surgery was examined for the presence of bacteria. twelve patients had sterile bile, 18 specimens of bile yielded anaerobes as well as aerobes, and 22 yielded aerobic bacteria only. escherichia coli was the most commonly isolated organism (30 strains). bacteroides fragilis was the most frequently encountered anaerobic bacterium and was found in 15 patients. the klebsiella-enterobacter group was the second most commonly iso ... | 1977 | 192815 |
| production and properties of theta-toxin of clostridium perfringens with special reference to lethal activity. | theta-toxin of clostridium perfringens produced in a synthetic medium showed high toxicity. the mouse was killed with 5-10 hemolytic units of the toxin. neutralization experiments showed that lethal and hemolytic activities were due to the same toxic entity. the amount of theta-toxin expressed in lethal activity reached more than 30% that of alpha-toxin in the synthetic medium sm67. although the activities of alpha-and theta-toxins were not additive in terms of ld50, increase in the ratio of the ... | 1976 | 192930 |
| [chromatograms of products of pyrolysis in the differentiation of clostridium perfringens a]. | | 1977 | 192975 |
| [demonstration and type determination of clostridium perfringens isolated from meat semipreserves]. | investigated were a total of 92 samples of pasteurized cans of different batches. six strains of clostridium perfringens were isolated from a sample of pasteurized ham. to demonstrate a toxin and present its type differentiation a strain was investigated with the use of dermonecrotic test after williams along with the cross toxin neutralization after the internationally accepted method, and the neutralization of the lecithinase activity after williams. the use of native toxins at the type differ ... | 1976 | 193241 |
| [presence and pathogenic significance of clostridium perfringens in the calf. 2. infection studies, ligation test and testing for enterotoxin formation for clarification of the pathogenic significance of clostridium perfringens type a]. | | 1977 | 193319 |
| [use of the passive hemagglutination test for the purpose of determining antibody levels following animal immunization with cl. perfringens toxoid]. | data are presented on the study of possibilities of the application of the passive hemagglutination test for titration of the blood sera of mice, guinea pigs and rabbits immunized with cl. perfringens toxoid. a diagnostic agent obtained by the sensitization of formalin- and tannin-treated sheep erythrocytes with the serologically pure toxoid, and homologous sera (as standard) were used in this test. a high immune response of balb/c and c3h mice to the cl. perfringens toxoid permits to suggest in ... | 1977 | 193324 |
| susceptibility of clostridium perfringens isolated from human infections to twenty antibiotics. | the proper choice of antibiotic for clostridium perfringens infections in patients allergic to penicillin is not clear; the usual recommendations and recent in vitro studies disagree. we tested the susceptibility of 57 strains of c. perfringens to eight penicillins, seven cephalosporins, two tetracyclines, clindamycin, chloramphenicol, and rifampin by the agar dilution method. all strains were inhibited by (per milliliter) 4 mug or less of any of the penicillins, chloramphenicol, or clindamycin ... | 1977 | 193441 |
| effects of phospholipases on selected renal enzymes: a histochemical study. | | 1977 | 193724 |
| serotypes, sporulation and enteropathogenicity of clostridium perfringens of faecal origin isolated in delhi area. | | 1977 | 193784 |
| outbreaks of food poisoning attributed to lecithinase-negative clostridium welchii. | | 1977 | 193875 |
| specificity of dna basic polypeptide interactions. ii+ influence of aromatic amino acid residues investigated with agarose bound lysine copolypeptides. | binding affinities towards dna and base pair specificities of lysine copolymers, containing different amounts of phe, tyr or trp residues, were estimated using a previously described chromatographic method. incorporation of few aromatic residues into polylysine causes a decrease in the binding affinity, however, further raising the aromatic residue - lysine ratio results in a continous increase of affinity, which is most pronounced with the tyr copolymers and not observed with polymers containin ... | 1977 | 194223 |
| [alpha-toxin (phospholipase c) of clostridium perfringens (author's transl)]. | | 1976 | 194279 |
| [properties and hemolytic action of clostridium perfringens theta-toxin (author's transl)]. | | 1976 | 194280 |
| [complementation of theta-and kappa-toxin production between two group mutants of clostridium perfringens (author's transl)]. | | 1976 | 194281 |