| a protocol and guide for the in vivo rat hepatocyte dna-repair assay. | the in vivo rat hepatocyte dna-repair assay is a valuable tool in assessing the genotoxic activity of chemical agents. an advantage of the system is that it reflects the complex patterns of uptake, distribution, metabolism, detoxification and excretion that actually occur in the whole animal. this article provides a typical procedure and guidelines for conducting the rat in vivo hepatocyte dna-repair assay. | 1987 | 3657828 |
| effect of egg as a sole diet on hepatic lipid peroxidation and related defence system in the rat. | | 1987 | 3666776 |
| short-term effects of two fusarium toxins, diacetoxyscirpenol and neosolaniol monoacetate, in male wistar rats. | this study compared the pathological and haematological effects of neosolaniol monoacetate in male wistar rats with those of diacetoxyscirpenol, a highly toxic trichothecene mycotoxin. both toxins caused statistically significant decreases in erythrocyte counts and increased the proportion of larger platelets compared to controls when dosed at 1 mg/kg body weight three times/wk for up to 5 wk. lesions caused by treatment with either toxin at this dosage level were mild and were seen--mainly afte ... | 1987 | 3679023 |
| practolol inhibits human skin fibroblast cell mat hydroxyproline accumulation. | despite being poorly absorbed practolol (n-4-2-hydroxy-3-(1-methyl-ethyl)-amino propoxy phenyl acetamine) inhibited the accumulation of cell mat hydroxyproline, a measure of collagen synthesis, by human skin fibroblasts (dt2ph) in vitro, (id50 0.8 x 10(-3) m). the degree of inhibition was dependent on the concentration of practolol used and the incubation time. neither preinitiation of collagen synthesis nor omitting ascorbic acid from the incubation medium modified this inhibitory action. in co ... | 1987 | 3687592 |
| na+-independent, pyridine nucleotide-linked efflux of ca2+ from preloaded rat heart mitochondria: induction by chlortetracycline. | | 1987 | 3689435 |
| na+-independent, pyridine nucleotide-linked efflux of ca2+ from preloaded rat heart mitochondria: induction by chlortetracycline. | | 1987 | 3689435 |
| [iron, manganese and cobalt levels in soil, pasture grass and bodies of young bulls after irrigation of the soil with waste water]. | the effect of irrigation of meadows with the water of the river. ner on the levels of iron, manganese and cobalt in the soil and vegetation was studied. experiments were also carried out on 30 young bulls fed with the hay grown on these meadows. the levels of iron and cobalt were determined in the blood plasma, and the manganese level in the hair of the bulls. the experiments were carried out twice during 93 days. the animals were divided into 3 groups. group i and ii received the hay from the i ... | 1987 | 3697343 |
| bidirectional cardiovascular connections between ventrolateral medulla and nucleus of the solitary tract. | single-unit recording experiments were done in chloralose-anesthetized, paralyzed and artificially ventilated cats to identify neurons in ventrolateral medulla (vlm) that send efferent axons directly to the region of the nucleus of the solitary tract (nts) and receive cardiovascular afferent inputs from the carotid sinus (csn) and aortic depressor (adn) nerves and the nts. units in vlm were identified by antidromic excitation to stimulation of functionally and histologically verified sites in th ... | 1986 | 3697701 |
| demonstration of sensory innervation of rat tongue with anterogradely transported horseradish peroxidase. | wheat germ agglutinin-horseradish peroxidase injected into the petrosal ganglion in rats was transported to the posterior tongue in the glossopharyngeal nerve. labelled fibers innervated the ipsilateral foliate papilla only, but both ipsi-and contralateral sides of the single circumvallate papilla. somatosensory fibers in the posterior tongue also were labelled. label crossed transcellularly from the afferent fibers into taste bud cells. use of this method provides more detailed knowledge of sen ... | 1986 | 3697712 |
| homologous cholinergic efferents spared by partial fimbrial lesions contribute to the recovery of hippocampal cholinergic enzymes in adult rats. | cholinergic fibers spared by partial lesions of the fimbrial bundle contribute to the recovery of enzyme markers for hippocampal cholinergic terminals. this recovery, most likely representing structural restoration of cholinergic terminals lost to lesion-induced degeneration, is amplified by a 'conditioning' lesion. this model of functionally relevant postlesion reconstruction, by and of structures within the cns, is suitable to search for means to enhance homotypic collateral sprouting. | 1986 | 3697713 |
| improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells. functional evaluation of monocytes in responses to lectins. | we have compared different techniques for the enrichment and depletion of monocytes from bovine peripheral blood mononuclear cells. adherence to plasma-coated gelatin was the most efficient and reproducible method for enrichment of monocytes (80% monocytes), whereas depletion of peripheral blood mononuclear cells of monocytes (0.3% monocytes and less) was best achieved by defibrination of the blood from which the pbm were separated. in both instances, purity of the cell population could be impro ... | 1986 | 3701072 |
| lamina viii interneurones interposed in crossed reflex pathways in the cat. | the location of a group of interneurones projecting to contralateral motor nuclei has been established using retrograde transneuronal transport of horseradish peroxidase conjugated with wheat germ agglutinin (wga-hrp). after labelling the motoneurones of semitendinosus, medial gastrocnemius or quadriceps muscles, interneurones which were secondarily labelled were found in lamina viii and in the neighbouring narrow strip of lamina vii. they were found to be distributed from the 4th lumbar to the ... | 1986 | 3701648 |
| [lithotripsy by ultrashort pulsation. experimental study in renal lithiasis in the dog]. | a lithotriptor delivering ultrashort shockwaves and designed for use under ultrasonographic guidance has recently been developed. immersion in water is unnecessary. the device was experimented in renal lithiasis in dogs. twenty-two animals were treated. in seven, who served as controls, the safety of shots delivered in particular to the lungs, liver, bones and kidneys was demonstrated. furthermore, no cardiac rhythm disorders were recorded. a human stone was implanted in 15 animals. the stone wa ... | 1986 | 3707073 |
| absence of role of endothelium in the response of isolated porcine coronary arteries to acetylcholine. | isolated precontracted arteries of various vascular beds relax in response to acetylcholine only if the endothelium is present. one explanation for this is that this drug stimulates the endothelial cells to release a vasodilator substance that in turn relaxes the underlying smooth muscle. to determine whether this mechanism is concerned also in the acetylcholine contraction of isolated porcine coronary arteries transverse strips of the extramural part of the left circumflex artery were used for ... | 1986 | 3719611 |
| antibodies to bacterial vaccines demonstrating specificity for human choriogonadotropin (hcg) and immunochemical detection of hcg-like factor in subcellular bacterial fractions. | investigations were done to determine whether vaccines prepared with chemically killed staphylococcus haemolyticus ru1 and streptococcus bovis av46 (bacteria that have been demonstrated to express human choriogonadotropin [hcg]-like material on their surface) elicited antibodies in rabbits with specificity for hcg determinants. in addition, the anatomical locus of the hcg-like factor was determined by separation of bacterial subcellular fractions. the results demonstrated that these bacterial va ... | 1986 | 3721581 |
| the sporogonic cycle of plasmodium reichenowi. | plasmodium reichenowi, a malarial parasite of the chimpanzee, was infective to anopheles freeborni, anopheles quadrimaculatus, anopheles stephensi, anopheles maculatus, anopheles dirus, and anopheles culicifacies mosquitoes. anopheles gambiae and anopheles albimanus were not infected. mean oocyst diameters of p. reichenowi were smaller than those of the other chimpanzee parasite, plasmodium schwetzi. sporozoites were present in the salivary glands of an. freeborni at 15 days when held at 25 to 2 ... | 1986 | 3734994 |
| analysis of factors involved in determining urinary bladder voiding cycle in urethan-anesthetized rats. | the mechanism(s) involved in determining the voiding cycle of the rat urinary bladder have been investigated in urethan-anesthetized animals. fluid emission is almost confined to that phase of the voiding cycle which is characterized by the presence of a series of high-frequency oscillations in intraluminal pressure (iphfo). during this phase the mean urethral flow rate reached a maximum and fluid was expelled in a stream-like fashion. the index obtained by multiplying the amplitude of iphfo by ... | 1986 | 3740305 |
| [salmonella agona infections in infants]. | | 1986 | 3748670 |
| a rapid assay for activity of phospholipase a2 using radioactive substrate. | a rapid method for the assay of phospholipase a2 has been developed using a radioactive substrate, l-alpha-dipalmitoyl-(2-[9,10(n)-3h]palmitoyl)-phosphatidylcholine. the substrate diluted with cold carrier (1 mm) is dissolved in 80% ethanol containing 25 mm sodium deoxycholate. the enzymatic reaction is performed in 1.0 ml 0.1 m glycine-naoh buffer, ph 9.0, containing 2 mumol cacl2, 10 micrograms bovine serum albumin, 2.5 mumol sodium deoxycholate, 0.01 unit (or less) phospholipase a2, and 40-10 ... | 1986 | 3755302 |
| linkage analysis of loci controlling blood groups and the rectovaginal constriction syndrome in jersey cattle. | the possibility of linkage between the recessive gene controlling the rectovaginal constriction (rvc) syndrome in jersey cattle and 13 loci controlling blood groups and polymorphic proteins was studied. no evidence of close to moderate linkage was found between the rvc locus and any of the systems a, b, c, f, l, s, z, r', hb, tf, am-1 and ca. no definite conclusion was possible with the m system. | 1986 | 3767084 |
| advances in contrast two-dimensional echocardiography. | | 1986 | 3768926 |
| the effects of large doses of methylprednisolone on neurologic recovery and survival in the mongolian gerbil following three hours of unilateral carotid occlusion. | using a scoring system designed to assess the severity of neurologic deficit in gerbils during and after temporary unilateral carotid occlusion, the effects of large doses of methylprednisolone sodium succinate (mpss: solu-medrol sterile powder) on experimental stroke have been examined. by scoring gerbils hourly for torso curvature, circling, inability to walk, ptosis, barrel rolling, opisthotonus, generalized seizures, and loss of righting reflex, their level of neurologic deficit can be easil ... | 1986 | 3768981 |
| survival of adrenal gland implants in the neocortex of the rat: a morphological study. | the survival of adrenal gland implantation in the cerebral cortex of the rat was studied. in the present study, mature adrenal gland had survived after six months of implantation. no scar tissue was observed between the adrenal gland cortex and the host cerebral cortex. the implanted tissue showed some reorganization in its cortex and medulla. in the adrenal cortex there was an observable increase in connective tissue fibers and some degeneration of cells. in the medulla, again, both surviving a ... | 1986 | 3780938 |
| intra-nucleus accumbens amphetamine: dose-dependent effects on food intake. | the effects of microinjections into the nucleus accumbens (n.acc.) of 0.0, 2.0 or 8.0 micrograms of (+)-amphetamine sulphate (amph) on food intake and running wheel activity were examined. the 2.0 micrograms dose of amph produced increased food intake while 8.0 micrograms significantly decreased food intake. no effect was found on running wheel activity with the 2.0 micrograms dose, though 8.0 micrograms significantly increased the number of wheel revolutions with respect to the saline group. re ... | 1986 | 3809216 |
| [a limiting factor of glycolysis in bone and callus]. | phosphofructokinase reaction was shown to be a key step of glycolysis after addition of various substrates to postmitochondrial fraction of bone and callus in vitro. administration of fructose-1,6-diphosphate into the animals with femur fracture within a first week of the fracture consolidation led to activation of glycolysis in regenerate as a result of which more mature callus was formed within 10 weeks. | 1986 | 3811293 |
| expression of agrobacterium tumefaciens t-dna gene 7 in xenopus laevis oocytes. | the expression of agrobacterium tumefaciens t-dna gene 7 was investigated in xenopus laevis oocytes. cloned dna injected into oocytes consisted of t-dna sequences derived from octopine type ti plasmid b6-806 and t-dna attached to plant dna sequences at the left junction in crown gall tumors. transcription initiation sites observed in oocytes were similar to those for transcript 7 in crown gall tumors. quantitative differences in transcription occurred depending on the flanking sequences of the i ... | 1986 | 3814121 |
| biosynthesis of 1-alkenes in higher plants. a model study with the composite carthamus tinctorius l. | uneven numbered 1-alkenes, such as 1-pentadecene, 1-heptadecene, 1,8-heptadecadiene, 1,8,11-heptadecatriene and 1,8,11,14-heptadecatetraene are the predominant hydrocarbons in germinating safflower (carthamus tinctorius l.). according to their chain length and positions of double bonds they are derived from palmitic, stearic, oleic, linoleic or linolenic acid, respectively. this has been proved by administration of synthetic alpha-, beta-, or gamma-deuterated 12-phenyldodecanoic acids to germina ... | 1987 | 3816783 |
| a new partition thin-layer chromatographic method for steroid separations. | tlc plates with a 25 mu thick polyamide stationary phase were modified for the separation of neutral steroids by impregnation with propylene glycol. a mixture of tritiated 5 alpha-androstan-3 alpha,17 beta-diol, testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one and 4-androstene-3,17-dione was applied to the plate and developed in a toluene mobile phase to a height of 13.6 cm. this resulted in complete resolution of the 4 compounds as detected by a gas flow scanner or imaging analyzer. cuttin ... | 1985 | 3835732 |
| powerline frequency electric and magnetic fields: a pilot study of risk perception. | the perception of the potential risk arising from human exposure to 50/60 hz electric and magnetic fields was studied with a quasi-random sample of 116 well-educated, opinion leaders using the risk perception framework previously developed by slovic, fischhoff, and lichtenstein. these individuals rated exposure to fields from transmission lines and electric blankets on a variety of scales that have been found useful in characterizing people's risk attitudes and perceptions. these judgments allow ... | 1985 | 3843677 |
| [candida albicans endophthalmitis in a heroin addict]. | | 1985 | 3879749 |
| [analysis of cell surface antigens of gastric cancer and preliminary study of clinical application by using mouse monoclonal antibodies produced against nugc3]. | four monoclonal antibodies (gc301, 302, 303, 304) produced by hybridomas obtained from mice immunized with nugc3 were analyzed by serological assay (mixed hemadsorption assay-mha) and immunoperoxidase technique. gc301(igg1) showed tumor restricted reactivity in serological analysis, but it reacted with fibrous interstitial tissues immunohistochemically. gc302(igg1) was serologically reactive with epithelial tumors such as gastric cancers, colon cancers and gynecological cancers, but not with bra ... | 1985 | 3889584 |
| quantitative analysis of in situ hybridization methods for the detection of actin gene expression. | we have implemented an efficient, quantitative approach for the optimization of in situ hybridization using double-stranded recombinant dna probes. the model system studied was actin mrna expression in chicken embryonic muscle cultures. actin and control (pbr322) probes were nick-translated with p32 labeled nucleotides, hybridized to cells grown on coverslips, and quantitated in a scintillation counter. cellular rna retention was monitored via the incorporation of h3-uridine into rna prior to ce ... | 1985 | 3889842 |
| quantitative analysis of in situ hybridization methods for the detection of actin gene expression. | we have implemented an efficient, quantitative approach for the optimization of in situ hybridization using double-stranded recombinant dna probes. the model system studied was actin mrna expression in chicken embryonic muscle cultures. actin and control (pbr322) probes were nick-translated with p32 labeled nucleotides, hybridized to cells grown on coverslips, and quantitated in a scintillation counter. cellular rna retention was monitored via the incorporation of h3-uridine into rna prior to ce ... | 1985 | 3889842 |
| [bacterial contamination of pump-collected breast milk]. | breast feeding of premature, new-born babies is often difficult when they become sick and are transferred to a children's hospital. the mother's milk should be collected and provided to the child without a loss of quality or too high a bacterial count which could endanger his health. we collected 113 samples of milk using electric vacuum breast pumps and then determined the bacterial contamination. in most cases there was evidence of bacteria normally found on the skin although more than 10(5)/m ... | 1985 | 3900703 |
| [serology of mediterranean boutonneuse fever. kinetics of antibodies detected by 3 methods: indirect immunofluorescence, indirect hemagglutination and latex agglutination]. | the purpose of this work was to determine the kinetics of antibodies in mediterranean spotted fever as determined by three serologic methods: indirect fluorescent antibody test, latex agglutination test and indirect hemagglutination test. no difference was noticed in the early kinetics but after 6 months, the latex agglutination test and the indirect hemagglutination test did not detect antibodies but the indirect fluorescent antibody test was still positive. this reaction is convenient for sero ... | 1985 | 3903623 |
| plasmid-mediated resistance to lincomycin by inactivation in staphylococcus haemolyticus. | staphylococcus haemolyticus bm4610 was resistant to high levels of lincomycin and susceptible to macrolides, clindamycin, and streptogramins. this resistance phenotype, not previously reported for a human clinical isolate, was due to inactivation of the antibiotic. the gene conferring resistance to lincomycin in strain bm4610 was carried by a 2.5-kilobase plasmid, pip855, which was cloned in escherichia coli. plasmid pip855 caused inactivation of both lincomycin and clindamycin in s. haemolyticu ... | 1985 | 3907492 |
| the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background. | n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ... | 1985 | 3911030 |
| comparison of bacteriostatic and bactericidal inhibitors of neisseria gonorrhoeae growth produced in vitro by urogenital staphylococci. | coagulase-negative staphylococci are known to produce bactericidal and bacteriostatic antigonococcal activities. out of 12 staphylococci, staphylococcus haemolyticus (four isolates) was identified as the principal source of bactericidal activity, whereas staphylococcus epidermidis (six isolates) was primarily responsible for producing bacteriostatic activity. a comparison of the bacteriostatic substance produced by s. epidermidis isolate 66 with the bactericidal substance produced by s. haemolyt ... | 1985 | 3924654 |
| the effect of estrogen treatment on plasma concentrations of steroid hormones, gonadotropins, prolactin and sex hormone-binding globulin in post-menopausal women. | twenty-one post-menopausal women on no other medication were treated with a low dose (0.625 mg/day) of conjugated equine estrogen (cee) for a mean (+/- sem) period of 2.6 +/- 0.2 mth (range 1.75-4.75). blood samples were collected before and at the completion of therapy, and alterations in the levels of prolactin (prl), follicle-stimulating hormone (fsh), luteinizing hormone (lh), sex hormone-binding globulin (shbg) and certain steroid hormones, including the free testosterone (t) index (t/shbg) ... | 1985 | 3929023 |
| the effect of estrogen treatment on plasma concentrations of steroid hormones, gonadotropins, prolactin and sex hormone-binding globulin in post-menopausal women. | twenty-one post-menopausal women on no other medication were treated with a low dose (0.625 mg/day) of conjugated equine estrogen (cee) for a mean (+/- sem) period of 2.6 +/- 0.2 mth (range 1.75-4.75). blood samples were collected before and at the completion of therapy, and alterations in the levels of prolactin (prl), follicle-stimulating hormone (fsh), luteinizing hormone (lh), sex hormone-binding globulin (shbg) and certain steroid hormones, including the free testosterone (t) index (t/shbg) ... | 1985 | 3929023 |
| prostaglandin-related bone resorption in cultured neonatal mouse calvaria: evaluation of biopotency of nonsteroidal anti-inflammatory drugs. | the objective of this study was the development of an assay based on suppression of endogenous prostaglandin synthesis in cultured neonatal mouse calvaria for evaluation of the biopotency of nonsteroidal anti-inflammatory drugs in bone. in preliminary trials, osteolytic activity due to spontaneous prostaglandin production over a 72 h culture period was found highly variable, and could not be stabilized by addition of the common precursor arachidonic acid to the culture medium. eventually, contin ... | 1985 | 3936122 |
| prostaglandin-related bone resorption in cultured neonatal mouse calvaria: evaluation of biopotency of nonsteroidal anti-inflammatory drugs. | the objective of this study was the development of an assay based on suppression of endogenous prostaglandin synthesis in cultured neonatal mouse calvaria for evaluation of the biopotency of nonsteroidal anti-inflammatory drugs in bone. in preliminary trials, osteolytic activity due to spontaneous prostaglandin production over a 72 h culture period was found highly variable, and could not be stabilized by addition of the common precursor arachidonic acid to the culture medium. eventually, contin ... | 1985 | 3936122 |
| identification of coagulase-negative staphylococci isolated from urinary tract infections. | coagulase-negative staphylococci isolated from urinary tract infections were identified using the api staph-ident system. organisms were excluded if there was no sign of pyuria or if normal urethral flora was present in significant amounts. while staphylococcus saprophyticus and staphylococcus epidermidis accounted for 81% of the isolates from females, 87% of isolates from males were s. epidermidis, staphylococcus warneri, or staphylococcus haemolyticus. the females fell into two main age groups ... | 1986 | 3940428 |
| value of the l-pyrrolidonyl-beta-naphthylamide hydrolysis test for identification of select gram-positive cocci. | a 4 hr test for hydrolysis of l-pyrrolidonyl-beta-naphthylamide (pyr) was evaluated for its use in identifying group a beta-hemolytic streptococci, group d enterococci, and staphylococcus haemolyticus. all of the group a streptococci and the enterococci tested were positive in the pyr test, whereas all of the nongroup a, b, or d beta-hemolytic streptococci, all of the viridans, and all of the streptococcus pneumoniae isolates tested were pyr negative. two strains of aerococcus species were pyr p ... | 1986 | 3943290 |
| composition and antimicrobic resistance of skin flora in hospitalized and healthy adults. | the aerobic bacterial flora of the skin of the anterior nares, axilla, perineum, and toe web in a group of 37 patients hospitalized for at least 2 weeks was compared with the flora of 30 healthy adults. colony counts were significantly higher for flora of the perineum and toe web in both groups (p less than 0.05). patients had significantly higher carriage rates of proteus, pseudomonas, and candida spp. (p less than 0.01). staphylococcus haemolyticus was isolated more frequently from patients, a ... | 1986 | 3958149 |
| embryo research. | | 1986 | 3960103 |
| heparinlike molecules with anticoagulant activity are synthesized by cultured endothelial cells. | cultured microvascular endothelial cells isolated from rat epididymal fat pads produce glycosaminoglycans that accelerate thrombin-antithrombin complex formation. the heparinlike nature of these macromolecules was established by complete destruction of their anticoagulant activity employing purified flavobacterium heparinase. only 15% of the biologic activity of these complex carbohydrates was expressed when the heparin binding domain on the protease inhibitor was chemically modified at the trp ... | 1985 | 3970699 |
| psychogenic colic in a horse. | a 4-year-old mixed-breed gelding was presented with colic-type behavior of 5 days' duration that included pawing, looking at the flank area, and kicking and biting at the abdomen and flank when it was eating hay or grain. medical evaluation did not reveal the cause of the "colic." during its stay in the hospital, the horse improved as regarded the colic-type behavior while eating, but resumed this behavior when it was able to see hay that it was not allowed to eat. it was determined that the per ... | 1985 | 3972697 |
| glycosaminoglycans of rat cerebellum: ii. a developmental study. | total and individual glycosaminoglycans (gags) were determined in rat cerebellum in tissue explants at various postnatal ages. the major constituents of gags were chondroitin sulfate (cs), hyaluronic acid (ha), and heparan sulfate (hs). dermatan sulfate (ds) and keratan sulfate (ks) could not be detected and therefore each amounts to less than 5% of all gags at all ages studied. ha was the prominent gag during postnatal development and only a minor constituent at adult ages, whereas cs was the p ... | 1985 | 3973596 |
| influence of housing conditions on pregnancy outcome in cynomolgus monkeys (macaca fascicularis). | female cynomolgus monkeys (macaca fascicularis) were kept under 3 different housing conditions: individually in type a cages (45 x 45 x 60 cm), individually in type b cages (70 x 70 x 100 cm) and as couples in type b cages. primigravida did not show early embryonic mortality, differing significantly from 11.5% early losses in multigravida. early embryonic mortality was not affected by housing condition. further reproductive failure rates did not differ significantly for primigravid (18.5%) and m ... | 1985 | 3974197 |
| workers' rights in occupational health. | | 1985 | 4014003 |
| workers' rights in occupational health. | | 1985 | 4014003 |
| resistance to extinction after schedules of partial delay or partial reinforcement in rats with hippocampal lesions. | two experimental procedures were employed to establish the reason why hippocampal lesions apparently block the development of tolerance for aversive events in partial reinforcement experiments, but do not do so in partial punishment experiments. rats were trained to run in a straight alley following hippocampal lesions (hc), cortical control lesions (cc) or sham operations (so), and resistance to extinction was assessed following differing acquisition conditions. in experiment 1 a 4-8 min inter- ... | 1985 | 4029302 |
| amikacin therapy for pseudomonas cellulitis in an amazon parrot. | amikacin sulfate, an aminoglycoside antibiotic, was used successfully to treat a case of severe pseudomonas cellulitus in an amazon parrot. side effects of polyuria and polydipsia occurred, but resolved without treatment 3 weeks after the antibiotic regimen. amikacin sulfate was given at a dosage of 0.04 mg/g of body weight, im, twice a day, based on antimicrobial sensitivity results. amikacin sulfate is indicated for p aeruginosa cellulitis that is resistant to gentamicin sulfate. although it i ... | 1985 | 4030478 |
| interactions of nuclear proteins with dna, during sperm differentiation in the ram. | ram spermatid nuclei and caput epididymal sperm nuclei were prepared and treated with dtt under conditions avoiding proteolysis. whole-mount preparations for the electron microscope were made in the presence or absence of the detergent joy. the chromatin of the less mature, non-round spermatid nuclei displayed a nucleosomal organization that gradually disappeared at the time the histones leave the nuclei (elongating spermatids). digestion with micrococcal nuclease suggests that polynucleosome ar ... | 1985 | 4042771 |
| hyperbaric pressure of 51 atmospheres is without effect on the depression of oxygen uptake in kidney tissue culture produced by halothane. | although anesthetized animals are awakened when subjected to increased pressure, compression does not result in antagonism of all phenomena associated with these drugs. it has recently been demonstrated that halothane's inhibition of respiration of isolated rat liver mitochondria is not reversed by hydraulic compression to 51 atmospheres. in order to determine whether this phenomenon can be extrapolated to the whole cell, we have investigated the effect of hydraulic compression of intact renal c ... | 1985 | 4046731 |
| free radical species mediating the toxicity of ozone for cultured rat lung fibroblasts. | we have reported a model system with which to study factors influencing the toxicity of o3 for cultured rat lung cells. this continuing investigation was to determine the relative contributions of free radical species to the toxicity of o3 as measured by 51cr release and the use of free radical scavengers. toxicity from modes of o3 exposure favoring stable free radicals (stationary cultures or added o3-exposed medium) was prevented by the h2o2 scavenger, catalase. toxicity produced by exposing c ... | 1985 | 4049433 |
| [cell electrophoretic studies of cattle lymphocytes]. | | 1985 | 4051667 |
| interaction of ultraviolet laser light with the cornea. | the effect of pulsed ultraviolet (uv) laser light on the cornea depends on wavelength (photon energy), irradiance (photon flux), and pulse firing rate. at the available excimer laser wavelengths of 193, 249, 308, and 351 nanometers, the authors have varied the irradiance per pulse (10 to 2000 mj/cm2) as well a pulse frequency (1, 10, 25 hz) and determined the thresholds for coagulation and ablation of the corneal stroma. the latter ablative action creates a groove resembling an incision and was ... | 1985 | 4055287 |
| short-term, mixed-diet overfeeding in man: no evidence for "luxuskonsumption". | after 13 days of weight maintenance diet (13,720 +/- 620 kj/day, 40% fat, 15% protein, and 45% carbohydrate), five young men (71.3 +/- 7.1 kg, 181 +/- 8 cm; means +/- sd) were overfed for 9 days at 1.6 times their maintenance requirements (i.e., +8,010 kj/day). twenty-four-hour energy expenditure (24-h ee) and basal metabolic rate (bmr) were measured on three occasions, once after 10 days on the weight-maintenance diet and after 2 and 9 days of overfeeding. physical activity was monitored throug ... | 1985 | 4061637 |
| the suicide inactivation of ox liver short-chain acyl-coa dehydrogenase by propionyl-coa. formation of an fad adduct. | propionyl-coa gave an unexpectedly low turnover (0.03 s-1) and high km (153 microm) as a substrate for ox liver short-chain acyl-coa dehydrogenase (scad). on addition of an excess of propionyl-coa to scad the flavin a448 decreased to about 30% of its original value and the peak at 368 nm was replaced by one at 335 nm. the decrease in a448 exhibited first-order kinetics and correlated with a first-order decrease in the enzyme's catalytic activity to 22% of the initial value. the flavin, released ... | 1985 | 4062874 |
| [ultrastructural characteristics of the salivary glands of of the tick ixodes persulcatus. ii. type iii alveoli of the sucking female]. | electron microscope observations were conducted to study changes in the cellular structure of iiid type alveoli of feeding females of i. persulcatus. within the first 1-2 days of feeding granulosecreting glandular cells "d" and "e" get free of secretory inclusions. after that cells "d" degenerate completely and cells "e" undergo morphological transformations and start to fulfil osmoregulatory functions. from the third day of feeding cytoplasm of cells "e" and basal intersticial cells form a comp ... | 1985 | 4069743 |
| [drug susceptibility of staphylococci isolated from clinical specimens]. | eighty-eight strains of staphylococci were isolated from a variety of specimens collected from patients at our university hospital from june to august 1982. these strains were identified as staphylococcus aureus (86%), staphylococcus epidermidis (6.8%), staphylococcus haemolyticus (2.3%), staphylococcus capitis (2.3%), and staphylococcus simulans (1.1%). one strain could not be identified with certainty as a currently recognized species. a majority (70%) of the s. aureus strains was resistant to ... | 1985 | 4089343 |
| the action of cyanogen bromide on rabbit igg molecules of allotypes a11 and a12. | | 1970 | 4097709 |
| inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. | | 1972 | 4118124 |
| [a new salmonella species: s. goma:6,7:z 4 z 23 :z 6 ]. | | 1972 | 4123804 |
| virus-induced cytoplasmic membrane structures associated with semliki forest virus infection studied by the freeze-etching method. | intracellular membrane structures associated with the semliki forest virus replication process were studied from freeze-etch replicas. cleaved membrane structures inside the cpv i type vacuoles lacked the typical membrane particles present on most other fractured membranes. cpv ii type vacuoles present in thin sections were obscured in the freeze-etch replicas by the cytoplasmic ground substance. | 1974 | 4129842 |
| [formation of microgranulomas in the bone marrow]. | | 1965 | 4158282 |
| localization in the kidney of antibodies to human skin and malignant melanoma. | | 1966 | 4161935 |
| effect of bacterial flora on iron absorption in the rat. | | 1968 | 4178110 |
| a study on the permeability barriers between disse's space and the bile canaliculus. | | 1969 | 4187928 |
| septic thrombophlebitis and venous cannulas. | | 1970 | 4194702 |
| some properties of the uridine diphosphate glucuronyltransferase activity synthesizing thio-beta-d-glucuronides. | 1. some properties of the udp-glucuronyltransferase synthesizing thio-beta-d-glucuronides were investigated and compared with those of the enzyme synthesizing the o-glucuronides of analogous phenols. 2. enzyme activity was generally similar for both classes of substrate in tissue distribution, intracellular location, optimum ph, perinatal development and induction by organ culture or by phenobarbital. 3. certain differences were noted between the two types of activity in behaviour on storage and ... | 1973 | 4198582 |
| plasmodium cynomolgi: influence of x-irradiation and sporozoite dilution on relapse patterns in infected rhesus monkeys. | | 1974 | 4206933 |
| ovulation induced by norepinephrine in rats made anovulatory by various experimental procedures. | | 1974 | 4215986 |
| what is your diagnosis? | | 1967 | 4226521 |
| acid glycosaminoglycans of pig aorta. | | 1967 | 4229079 |
| anabolic steroids in muscular, neurologic and hematologic disorders. | | 1968 | 4231282 |
| studies on the structure of the mitochondrial membranes. | | 1970 | 4245917 |
| [epizootiology of foot-and-mouth disease in kuban]. | | 1970 | 4249236 |
| hyperglycemia and starvation: contrast in rat brain gluconeogenic amino acids and glycogen. | | 1972 | 4264149 |
| [tissue adenosine triphosphatase activity of different sections of rat and rabbit brain in normal conditions and with administration of hydrocortisone]. | | 1973 | 4265282 |
| acyl carrier protein. ix. acyl carrier protein hydrolase. | | 1967 | 4290442 |
| cadmium-induced selective lesions of sensory ganglia. | | 1967 | 4290939 |
| cadmium-induced selective lesions of sensory ganglia. | | 1967 | 4290939 |
| testosterone metabolism by the chicken uropygial gland. | | 1967 | 4294227 |
| testosterone metabolism by the chicken uropygial gland. | | 1967 | 4294227 |
| [testosterone metabolism in man. peripheral testosterone metabolism]. | | 1967 | 4295365 |
| circadian rhythm and stress-induced changes in hypothalamic content of corticotropin-releasing activity during postnatal development in the rat. | | 1970 | 4314069 |
| studies on swine enteroviruses. japanese 6th serotype and relationship between heat susceptibility and ctyopathic effects. | | 1970 | 4315493 |
| [reproduction of experimental hepatitis in puppies]. | | 1970 | 4316401 |
| evidence for proteolytic degradation of histidinol phosphate phosphatase specified by nonsense mutants of the hisb gene of salmonella typhimurium. | the gene products from hisb nonsense mutants having histidinol phosphate phosphatase activity were isolated from salmonella typhimurium. the enzyme from strain tr691 (hisb278 hist1529 arod5) was isolated in the presence of diisopropylfluorophosphate. three electrophoretically separable forms were demonstrated, and all were shown to have a mol wt of approximately 38,000 and to consist of a single polypeptide chain. previously, two forms of the phosphatase enzyme from this strain were isolated wit ... | 1973 | 4355487 |
| isolation of clostridium absonum and its cultural and biochemical properties. | a new procedure for isolation of clostridium absonum was devised. sixtyseven strains of c. absonum were isolated from 135 soil samples, but no strain of c. absonum could be found from human fecal samples. the lecithinase, hemolysin, and lethal toxin in the culture filtrates of this species exhibited low avidity for c. perfringens type a antitoxin. the three activities were inseparable by the present method of purification. a reinvestigation of biochemical properties revealed that incomplete supp ... | 1974 | 4357934 |
| pteridine formation during lectin-induced lymphocyte activation. | after iodine oxidation, biopterin, 6-hydroxymethylpterin, and 6-formylpterin were identified in mouse spleen lymphocytes by means of reverse-phase hplc, crithidia assay, and oxidative degradation. concanavalin a activation induces a 30-fold increase in the pteridine amounts; biopterin as well as the sum of the carbinol and the aldehyde attain levels of 6-8 x 10(-12) mol/10(6) cells. the most rapid increase occurs during the first 24 hr. thus, pteridine accumulation precedes the period of lymphoc ... | 1985 | 4066775 |
| labeling of cysteine-containing peptides with 2-nitro-5-thiobenzoic acid. | a method for specific labeling of cysteine-containing peptides has been developed using ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb). prior to cleavage with proteases or chemical reagents, proteins are reacted with dtnb, resulting in the formation of a mixed disulfide between the protein sulfhydryl group and 2-nitro-5-thiobenzoic acid (tnb). the formation of the mixed disulfide introduces a chromophore, with an absorbance peak at 328 nm. by monitoring peptide maps generated by hp ... | 1985 | 4014680 |
| in vitro studies on the reactivation by oximes of phosphylated acetylcholinesterase--ii. on the formation of o,o-diethyl phosphorylated ache and o-ethyl methylphosphonylated ache and their reactivation by ps2. | the in vitro reactivation profiles of o,o-diethyl phosphorylated ache and o-ethyl methyl phosphonylated ache by p2s (2-hydroxy iminomethyl-1-methyl-pyridinium methane sulphonate) have been determined. whilst reinhibition of the reactivated ache by phosphorylated oxime (pox) is not important in determining the reactivation profile of o,o-diethyl phosphorylated ache, reinhibition of the reactivated ache by phosphonylated oxime can, however, be important in determining the reactivation profile of o ... | 1986 | 3954784 |
| human pancreatic growth hormone (gh)-releasing hormone stimulates gh synthesis and release in infant rats. an in vivo study. | administration of human pancreatic gh-releasing factor 1-40 (hpgrf-40) at doses of 1, 10, 20, 100, and 500 ng/100 g bw sc induced in 10-day-old rats a clear-cut rise in plasma gh 15-min post-injection, although the effect was not dose-related and peak gh levels were already present after the lowest grf dose. in 25-day-old rats, hpgrf induced only a slight rise in plasma gh at the dose of 500 ng/100 g bw sc, whereas it was completely ineffective at the lower doses. in 5-day-old rats, hpgrf (20 ng ... | 1985 | 3917905 |
| ly164846 in vitro antimicrobial activity testing, including disk diffusion susceptibility tests using 30-microgram disks. | a new oral cephalosporin, ly164846 (eli lilly & co., indianapolis, ind.), was found to have a unique antimicrobial spectrum confined to methicillin-susceptible staphylococcus spp., streptococci (except enterococcus spp.), haemophilus influenzae, branhamella catarrhalis, and some anaerobes. cephalothin in vitro tests (30-microgram disks or dilution) can represent ly164846 for laboratory testing if comparable interpretive breakpoints are applied to strains within the spectrum of ly164846. organism ... | 1986 | 3755018 |
| effect of metoclopramide on adrenal secretion in sheep: influence of dexamethasone and sodium intake. | metoclopramide, a competitive dopamine antagonist, stimulates aldosterone in man and monkey without affecting cortisol secretion. in sheep, metoclopramide also stimulates aldosterone but ist action on adrenocortical secretion is more controversial. to clarify the action of metoclopramide in conscious sheep, the response of plasma aldosterone, cortisol, angiotensin ii and potassium were studied after 0.16 and 0.64 mg/kg metoclopramide, with and without pretreatment with dexamethasone. the effect ... | 1987 | 3613572 |
| effect of metoclopramide on adrenal secretion in sheep: influence of dexamethasone and sodium intake. | metoclopramide, a competitive dopamine antagonist, stimulates aldosterone in man and monkey without affecting cortisol secretion. in sheep, metoclopramide also stimulates aldosterone but ist action on adrenocortical secretion is more controversial. to clarify the action of metoclopramide in conscious sheep, the response of plasma aldosterone, cortisol, angiotensin ii and potassium were studied after 0.16 and 0.64 mg/kg metoclopramide, with and without pretreatment with dexamethasone. the effect ... | 1987 | 3613572 |