| production of active pediocin pa-1 in escherichia coli using a thioredoxin gene fusion expression approach: cloning, expression, purification, and characterization. | antimicrobial peptides possess cationic and amphipathic properties that allow for interactions with the membrane of living cells. bacteriocins from lactic acid bacteria, in particular, are currently being studied for their potential use as food preservatives and for applications in health care. however, bacteriocin exploitation is often limited owing to low production yields. gene cloning and heterologous protein or peptide production is one way to possibly achieve overexpression of bacteriocins ... | 2007 | 18026219 |
| morphological changes induced in listeria monocytogenes v7 by a bacteriocin produced by pediococcus acidilactici. | pediococcus acidilactici produces bacteriocin, which kills listeria monocytogenes. the bactericidal mode of action of the bacteriocin against l. monocytogenes v7 was investigated by transmission electron microscopy. the bacteriocin was purified partially from the cell-free extract using micro-cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. the bacteriocin (6,400 au/ ml) was inoculated with l. monocytogenes v7 and incubated for 0.5 h, 1 h, 3 h, and 6 h. ... | 2007 | 18051280 |
| growth control of listeria innocua 2030c on vacuum-packaged cold-smoked salmon by lactic acid bacteria. | five bacteriocin-producing lactic acid bacteria (lab): enterococcus faecium et05, lactobacillus curvatus et06, l. curvatus et30, l. deldrueckii et32 and pediococcus acidilactici et34, selected by their capacity for growth and producing inhibition in vitro at high salt-on-water content, low temperature and anaerobic atmosphere, conditions simulating cold-smoked fish, were inoculated onto salmon fillets, in co-culture with listeria innocua 2030c, and cold-smoked processed (dry salted for 6 h; dryi ... | 2008 | 18068846 |
| pediocin sa-1, an antimicrobial peptide from pediococcus acidilactici nrrl b5627: production conditions, purification and characterization. | fermentation broths of pediococcus acidilactici nrrl b5627 exhibited a certain antimicrobial activity due to a bacteriocin produced during early growth and until the stationary phase of growth was reached (at optimum of 60% dissolved oxygen saturation). its size was determined by electrospray ionization mass spectrometric analysis as 3.660 kda. n-terminal sequencing showed that the bacteriocin had 19 amino acid residues in the order kyygxngvxtxgkhsxvdx. the purified bacteriocin is similar to ped ... | 2008 | 18093831 |
| chimeras of mature pediocin pa-1 fused to the signal peptide of enterocin p permits the cloning, production, and expression of pediocin pa-1 in lactococcus lactis. | chimeras of pediocin pa-1 (peda-1), a bacteriocin produced by pediococcus acidilactici plbh9, fused to the signal peptide of enterocin p (entp), a sec-dependent bacteriocin produced by enterococcus faecium p13, permitted the production of peda-1 in lactococcus lactis. chimeric genes encoding the entp signal peptide (sp(entp)) fused to mature peda-1 (peda), with or without its immunity gene (pedb), were cloned into the expression vector pmg36c to generate the recombinant plasmids pmpp9 (sp(entp): ... | 2007 | 18095432 |
| diversity of predominant lactic acid bacteria associated with cocoa fermentation in nigeria. | the fermentation of cocoa relies on a complex succession of bacteria and filamentous fungi, all of which can have an impact on cocoa flavor. so far, few investigations have focused on the diversity of lactic acid bacteria involved in cocoa fermentation, and many earlier investigations did not rely on polyphasic taxonomical approaches, which take both phenotypic and genotypic characterization techniques into account. in our study, we characterized predominant lactic acid bacteria from cocoa ferme ... | 2008 | 18213481 |
| rapid quantifiable assessment of nutritional parameters influencing pediocin production by pediococcus acidilactici nrrl b5627. | a direct plate bioassay procedure was applied for rapid and quantifiable assessment of the influence of various nutritional parameters on pediocin production by pediococcus acidilactici nrrl b5627. solid-state cultivation of the microorganism was done on mrs-based media over 3-and 6-hours incubation periods. nutritional parameters assessed included the carbon source (glucose, sucrose, fructose, galactose, glycerol), and various salts (nh(4)po(4), cacl(2), kh(2)po(4), mnso(4).h(2)o). glucose was ... | 2008 | 18215516 |
| detection of biogenic amine producer bacteria in a typical italian goat cheese. | the aim of this study was to research decarboxylating bacterial strains and biogenic amine content in a typical italian goat cheese (robiola di roccaverano). the study was performed on fresh and ripened samples of goat cheese manufactured from industrial and artisanal producers. sixty-seven bacterial strains isolated showed decarboxylating activity, and enterococcus faecalis was the most widespread decarboxylating species in all artisanal and industrial products. pediococcus acidilactici and ent ... | 2008 | 18236686 |
| capacity of human nisin- and pediocin-producing lactic acid bacteria to reduce intestinal colonization by vancomycin-resistant enterococci. | this study demonstrated the capacity of bacteriocin-producing lactic acid bacteria (lab) to reduce intestinal colonization by vancomycin-resistant enterococci (vre) in a mouse model. lactococcus lactis mm19 and pediococcus acidilactici mm33 are bacteriocin producers isolated from human feces. the bacteriocin secreted by p. acidilactici is identical to pediocin pa-1/ach, while pcr analysis demonstrated that l. lactis harbors the nisin z gene. lab were acid and bile tolerant when assayed under sim ... | 2008 | 18245231 |
| effects of orally administered probiotic pediococcus acidilactici on the small and large intestine of weaning piglets. a qualitative and quantitative micro-anatomical study. | probiotic research has been approached, above all in recent years, by widely differing points of view, both for human and animal uses. lactic acid bacteria release bacteriocins, and some of them may function as probiotic. the aim of the present study was to investigate the effects of dietary supplementation with the probiotic pediococcus acidilactici on the piglet intestine, on circulating lymphocytes, and on aspects of piglet performance during the first 42 days after weaning. sixteen female pi ... | 2008 | 18366003 |
| functional properties of selected starter cultures for sour maize bread. | this paper focuses on the functional properties of maize sour-dough microflora selected and tested for their use as starter cultures for sour maize bread. lactic acid bacteria and yeasts isolated from spontaneously fermented maize dough were selected based on dominance during fermentation and presence at the end of fermentation. functional properties examined included acidification, leavening and production of some antimicrobial compounds in the fermenting matrix. the organisms previously identi ... | 2008 | 18456117 |
| pediocin pa-1 production during repeated-cycle batch culture of immobilized pediococcus acidilactici ul5 cells. | pediocin pa-1 production by pediococcus acidilactici ul5 cells immobilized in kappa-carrageenan/locust bean gum gel beads was studied during repeated-cycle batch (rcb) culture with ph control in man rogosa and sharpe (mrs) broth supplemented with 1% glucose and whey permeate (swp) medium. the pediocin pa-1 production by free p. acidilactici cells ph-controlled batch culture has reached 2048 and 4096 au ml(-1) after 11 and 12 h of incubation, with volumetric productivities of 187 and 342 au ml(-1 ... | 2008 | 18558343 |
| immunity gene pedb enhances production of pediocin pa-1 in naturally resistant lactococcus lactis strains. | heterologous production of the antilisterial bacteriocin pediocin pa-1 in lactococci is an attractive objective to increase the safety of dairy products. in a previous paper, we developed a system for the heterologous production of the bacteriocin pediocin pa-1 in pediocin-resistant lactococcal hosts through a leader exchange strategy. the system was based on 3 genes, 1 encoding the fusion between the lactococcin a leader and propediocin pa-1, and the other 2 encoding the lactococcin a secretion ... | 2008 | 18565917 |
| effects of the administration of pediococcus acidilactici to laying hens on productive performance. | | 2008 | 18685970 |
| purification of pediocin pa-1 by immunoaffinity chromatography. | a one-step purification procedure for purifying pediocin pa-1, a class iia bacteriocin produced by pediococcus acidilactici ul5, was developed based on column immunoaffinity chromatography with specific antipediocin pa-1 polyclonal antibodies coupled to cyanogen bromide-activated sepharosetm. about 13.3 microg/ml purified pediocin pa-1 was obtained from 15 ml p. acidilactici ul5 culture supernatant, as measured by enzyme-linked immunosorbent assay. the specific activity and average recovery of t ... | 2008 | 18727543 |
| novel extracellular proteolytic activity in pediococcus acidilactici atcc 8042. | proteolytic systems are common in lactic acid bacteria, but there are few reports about proteases or peptidases in the genus pediococcus. to evaluate the presence of these types of enzymes, pediococcus acidilactici atcc 8042 was cultured in mrs broth. supernatants collected during the log phase showed proteolytic activity towards an elastin dispersion when assayed using a spectrophotometer. zn2+ showed a stimulatory effect, and the proteolytic activity reached its maximum when 200 mmol/l nacl wa ... | 2008 | 18772932 |
| isolation and characterization of acid-sensitive mutants of pediococcus acidilactici. | acid-sensitive mutants of pediococcus acidilactici bcc 9545, a starter culture of the thai fermented pork sausage nham, were isolated as spontaneous neomycin resistant mutants. the mutants generally produced less acid and acidified the culture media less than the parent strain in a 72 h culturing period. interestingly, the atpase activities of the mutants did not differ considerably from that of the parent strain in acidic conditions. it was also found that the internal ph values of the mutant s ... | 2009 | 19028310 |
| administration of pediococcus acidilactici or saccharomyces cerevisiae boulardii modulates development of porcine mucosal immunity and reduces intestinal bacterial translocation after escherichia coli challenge. | in this study, the influence of the probiotics, pediococcus acidilactici (pa) and saccharomyces cerevisiae boulardii (scb), on intestinal immune traits and resistance to enterotoxigenic escherichia coli (etec) infection was evaluated in pigs. two weeks before farrowing, 30 sows and their future litters were allocated to the following treatments: 1) control group without antibiotic or probiotic treatment (ctrl), 2) control with antibiotic (tiamulin) added to weanling feed (abt), or litters treate ... | 2009 | 19028865 |
| pediococcus argentinicus sp. nov. from argentinean fermented wheat flour and identification of pediococcus species by phes, rpoa and atpa sequence analysis. | a gram-positive, small coccus-shaped lactic acid bacterium, strain lmg 23999(t), was isolated from argentinean wheat flour. 16s rrna gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus pediococcus, with pediococcus stilesii, pediococcus pentosaceus and pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9 % gene sequence similarity, respectively). fluorescent amplified fragment length polymorphism fingerprinting of whole genome ... | 2008 | 19060081 |
| resolution of persistent pediococcus bacteremia with daptomycin treatment: case report and review of the literature. | a case of bacteremia caused by pediococcus acidilactici occurring in a patient with metastatic adenocarcinoma of the gallbladder is described. the bacteremia persisted despite an antimicrobial regimen of vancomycin, ciprofloxacin, metronidazole, and caspofungin. within 36 h of switching the vancomycin to daptomycin (6 mg/kg per day), the bacteremia resolved and the patient improved clinically. previous reports of human pediococcal infection are also reviewed. | 2010 | 19081696 |
| [study of genetic control of pediocins synthesis]. | plasmid profiles of 2 strains of pediococcus acidilactici and 3 strains of p. pentosaceus were studied. synthesis of bacteriocins in 2 strains of p. acidilactici was coded on the plasmid which was confirmed by experiment with elimination of plasmids and subsequent electrophoregram, which demonstrated synchronous disappearance of bacteriocins synthesis characteristic in plasmid-lacking variants. in 3 strains of p. pentosaceus plasmids were not found which points to chromosomal localization of gen ... | 2008 | 19189461 |
| isolation of bovine intestinal lactobacillus plantarum and pediococcus acidilactici with inhibitory activity against escherichia coli o157 and f5. | the growth rate of bovine lactic acid bacteria (lab) in five different culture conditions, and their inhibitory activity against escherichia coli o157 and f5 in two assays was assessed to identify lab for potential prophylactic use in cattle. | 2009 | 19200307 |
| isolation and identification of spoilage microorganisms using food-based media combined with rdna sequencing: ranch dressing as a model food. | investigating microbial spoilage of food is hampered by the lack of suitable growth media and protocols to characterize the causative agents. microbial spoilage of salad dressing is sporadic and relatively unpredictable, thus processors struggle to develop strategies to minimize or prevent spoilage of this product. the objectives of this study were to (i) induce and characterize spoilage events in ranch-style dressing as a model food, and (ii) isolate and identify the causative microorganisms us ... | 2009 | 19269562 |
| co-cultivation of a bacteriocin-producing mixed culture of bifidobacterium thermophilum rbl67 and pediococcus acidilactici uva1 isolated from baby faeces. | we investigated the kinetics of growth, metabolism and antimicrobial activity of bifidobacterium thermophilum rbl67 and pediococcus acidilactici uva1 previously isolated as a consortium from human baby faeces and producing antimicrobial proteinaceous compounds. | 2009 | 19298509 |
| production of shelf-stable ranch dressing using high-pressure processing. | high-pressure processing (hpp) can reduce or eliminate microorganisms of concern in food without deteriorating product quality; however, quality benefits must justify the substantial capital investment for the utilization of this technology. hpp is particularly a beneficial preservation technology for products damaged by thermal treatments or when product quality could be improved by reformulation to raise ph or eliminate chemical preservatives. the primary objectives of this study were to deter ... | 2009 | 19323763 |
| characterization and application of a native lactic acid bacterium isolated from tannery fleshings for fermentative bioconversion of tannery fleshings. | lactic acid bacteria (lab) species isolated from limed and delimed tannery fleshings (tf) were evaluated for their fermentation efficiency and antibacterial property. the native lab isolates efficiently fermented tf and resulted in a fermented mass with antioxidant properties, indicating their potential for effective eco-friendly bioconversion of tf. from among the lab isolated, a proteolytic isolate showing better antimicrobial spectrum and reasonably good fermentation efficiency was identified ... | 2009 | 19333593 |
| pediococcus lolii sp. nov., isolated from ryegrass silage. | a gram-positive, coccus-shaped, lactic acid bacterium, strain ngri 0510q(t), was isolated from ryegrass silage produced in okinawa prefecture, japan. the cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. the strain is homofermentative and produces d- and l-lactic acid from glucose. phylogenetic analysis based on 16s rrna gene sequences revealed that strain ngri 0510q(t) belongs to the genus pediococcus and clusters within the pediococcus acidilactici and pediococcus pentosac ... | 2009 | 19406783 |
| in vivo study on the effectiveness of pediocin pa-1 and pediococcus acidilactici ul5 at inhibiting listeria monocytogenes. | the anti-listerial effect of pediocin pa-1 and its producing strain, pediococcus acidilactici ul5, was investigated in vivo using an icr mouse model. the effect of intra-gastric administration of a single dose of p. acidilactici ul5 (4 x 10(10) cfu/animal) on the propagation of listeria monocytogenes lsd348 in intestine, liver and spleen was negligible. p. acidilactici ul5 did not appear competitive with the mouse intestinal flora and was not detectable in fecal samples collected two days after ... | 2009 | 19541383 |
| validation of ground-and-formed beef jerky processes using commercial lactic acid bacteria starter cultures as pathogen surrogates. | beef jerky has been linked to multiple outbreaks of salmonellosis and escherichia coli o157:h7 infection over the past 40 years. with increasing government scrutiny of jerky-making process lethality, a simple method by which processors can easily validate the lethality of their ground-and-formed beef jerky process against salmonella' and e. coli o157:h7 is greatly needed. previous research with whole-muscle beef jerky indicated that commercial lactic acid bacteria (lab) may be more heat resistan ... | 2009 | 19610334 |
| identification of lactic acid bacteria isolated from tarhana, a traditional turkish fermented food. | tarhana is a traditional fermented product produced from a mixture of spontaneously fermented yogurt and wheat flour in turkey. the aims of the present study were to enumerate and identify for the first time by molecular biology-based methods predominant lactic acid bacteria (lab) isolated during processing of tarhana. samples were collected from eight different regions of turkey. in order to explore the relationship between raw material and the microbiology of tarhana, yogurt and wheat flour we ... | 2009 | 19703719 |
| assessment of cell surface properties and adhesion potential of selected probiotic strains. | to evaluate the physicochemical cell surface and adhesive properties of selected probiotic strains for human use. | 2009 | 19725886 |
| pediocin production in milk by pediococcus acidilactici in co-culture with streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus. | the production of pediocin in milk by pediococcus acidilactici was evaluated in co-culture with the dairy fermentation cultures streptococcus thermophilus and lactobacillus delbrueckii ssp. bulgaricus. the cultures were tested singly and in different combinations in milk (0 or 2% fat content) during incubation at 40 degrees c for up to 10 h. cell-free milk samples taken every 60 min were tested for bacteriocin activity against listeria monocytogenes. pediocin activity was not detectable when p. ... | 2010 | 19844751 |
| genotypic diversity of lactic acid bacteria isolated from african traditional alkaline-fermented foods. | to identify and compare lactic acid bacteria (lab) isolated from alkaline fermentations of cassava (manihot esculenta crantz) leaves, roselle (hibiscus sabdariffa) and african locust bean (parkia biglobosa) seeds for production of, respectively, ntoba mbodi, bikalga and soumbala. | 2010 | 19895650 |
| pelagic fish hydrolysates as peptones for bacterial culture media. | for several years in the quebec fisheries' industry, landings of pelagic fish have been calculated at over 4000 tons. these under-exploited species, rich in lipids and proteins, could be used in valuable new products. in the present study, hydrolysates of mackerel and herring were produced and utilized as sources of peptones in the formulation of new bacterial culture media. the molecular weight distribution analysis showed that molecules present in the hydrolysates were lower than 1300 da for h ... | 2009 | 19940932 |
| effect of probiotic pediococcus acidilactici on antioxidant defences and oxidative stress of litopenaeus stylirostris under vibrio nigripulchritudo challenge. | antioxidant defences and induced oxidative stress tissue damage of the blue shrimp litopenaeus stylirostris, under challenge with vibrio nigripulchritudo, were investigated for a 72-h period. for this purpose, l. stylirostris were first infected by immersion with pathogenic v. nigripulchritudo strain sfn1 and then antioxidant defences: superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx), total antioxidant status (tas), glutathiones and induced tissue damage (mda and carbonyl ... | 2010 | 20045735 |
| study of the physicochemical and biological stability of pediocin pa-1 in the upper gastrointestinal tract conditions using a dynamic in vitro model. | to evaluate the survival of pediococcus acidilactici ul5 and its ability to produce pediocin pa-1 during transit in an artificial gastrointestinal tract (git). to investigate the physicochemical and biological stability of purified pediocin pa-1 under git conditions. | 2010 | 20059619 |
| effects of a probiotic intervention in acute canine gastroenteritis--a controlled clinical trial. | to evaluate the effect of a probiotic product in acute self-limiting gastroenteritis in dogs. | 2010 | 20137007 |
| the effect of pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (oreochromis niloticus). | aim: to assess pediococcus acidilactici as a dietary supplement for on-growing red tilapia (oreochromis niloticus). methods and results: tilapia were fed either a control diet or control diet supplemented with ped. acidilactici at 10(7) cfu g(-1) for 32 days. ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. pcr-dgge revealed direct antagonism of gastric ped. acidilactici with an endogenous uncultured bacterium during a period of re ... | 2010 | 20353430 |
| phenotypic and genetic heterogeneity of lactic acid bacteria isolated from "alheira", a traditional fermented sausage produced in portugal. | the aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (lab) isolated from "alheira", a fermented sausage produced in portugal. lab were identified to genus and species level by phenotypic characteristics, using genus or species-specific primers and sequencing of the gene encoding 16s rrna. two-hundred and eighty-three isolates were grouped into 14 species. lactobacillus plantarum was isolated from all sausages and enterococcusfaecalis from most of ... | 2009 | 20416703 |
| some growth parameters and antimicrobial activity of a bacteriocin-producing strain pediococcus acidilactici 13. | some growth parameters and bacteriocin production by the strain pediococcus acidilactici 13, were screened in this study. the inhibitor substance produced by this strain showed strong antimicrobial activity at 204,800 au/ml (activity units/ml) against the gram positive food borne pathogen, listeria monocytogenes, when the incubation temperature was 37 degrees c and the initial ph of the medium, tge (trypton glucose yeast extract), was 6.0. the bacteriocin-producing strain had considerable resist ... | 2010 | 20483184 |
| the chemical code of porcine enteric neurons and the number of enteric glial cells are altered by dietary probiotics. | the enteric nervous system (ens) contains chemically coded populations of neurons that serve specific functions for the control of the gastrointestinal tract. the ability of neurons to modify their chemical code in response to luminal changes has recently been discovered. it is possible that enteric neuronal plasticity may sustain the adaptability of the gut to changes in intestinal activity or injury, and that gut neurons may respond to an altered intestinal environment by changing their neurop ... | 2010 | 20524986 |
| development and analysis of microbial characteristics of an acidulocomposting system for the treatment of garbage and cattle manure. | an acidulocomposting system for the treatment of cattle manure with little emission of ammonia gas was developed, and the structure of its microbial community was investigated by denaturing gradient gel electrophoresis (dgge) and clone library construction. an acidulocomposting apparatus (bc20, 20 l) was operated for 79 days to treat 2 kg (wet wt) of garbage per 1 or 2 days. on day 80 of operation, the substrate was changed from garbage to cattle manure (1 kg of beef cattle manure was added to t ... | 2010 | 20547374 |
| pediococcus acidilactici isolated from the rumen of lambs with rumen acidosis, 16s rrna identification and sensibility to monensin and lasalocid. | a lactic-acid producing bacterium was isolated from the rumen of lambs with rumen acidosis. the cells were gram-positive, nonmotile, nonsporing, catalase negative spherical, 1.5-2.0 μm in diameter, and occur in pairs and tetrads. analysis of 16s ribosomal rna indicated that the rumen bacterium was a strain of pediococcus acidilactici with 99% of nucleotide homology. this bacterium was sensible to monensin and lasalocid at the unique dose tested of 300 ppm. the concentration of lactic acid and dm ... | 2011 | 20553885 |
| purification of bacterial genomic dna in less than 20 min using chelex-100 microwave: examples from strains of lactic acid bacteria isolated from soil samples. | we established a chelex 100-microwave method for the purification of bacterial genomic dna (gdna) in less than 20 min with high yield and good quality, useful for multiple purposes. it combines chelex 100, proteinase k, rnase a and heating in a microwave oven. the resulting gdna was used directly to identify bacterial species of the order lactobacillales by means of pcr amplification of their 16s rdna gene, isolated from sediments on the yucatan peninsula, mexico. this method produced gdna free ... | 2010 | 20556655 |
| diversity of lactic acid bacteria from hussuwa, a traditional african fermented sorghum food. | the diversity of lactic acid bacteria associated with hussuwa fermentation, a sudanese fermented sorghum food, was studied using a polyphasic taxonomical approach. predominant strains could be well characterised based on a combination of phenotypic tests and genotypic methods such as ardra, rep-pcr and rapd-pcr, as well as 16s rrna gene sequencing of representative strains. thus, the majority (128 of 220, 58.3%) of strains exhibited phenotypic properties typical of heterofermentative lactobacill ... | 2010 | 20630317 |
| microorganisms associated with maari, a baobab seed fermented product. | a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ... | 2010 | 20688411 |
| heterologous production of pediocin pa-1 in lactobacillus reuteri. | the recombinant dna plr5cat_psab in which pediocin pa-1 structural and immunity genes (pedab) fused with the promoter and deduced signal sequence of an alpha-amylase gene from a bifidobacterial strain were inserted in plr5cat, an escherichia coli-lactobacilli shuttle vector was transferred to lactobacillus reuteri kctc 3679 and the transformant presented bacteriocin activity. the recombinant l. reuteri kctc 3679 transformed with the shortened plr5cat(s)_psab, where non-essential region for the l ... | 2010 | 20798585 |
| identification of escherichia coli o157:h7 surrogate organisms to evaluate beef carcass intervention treatment efficacy. | we compared the survival of potential pathogen surrogates-meat-hygiene indicators (non-escherichia coli coliforms), biotype i e. coli, and lactic acid bacteria starter cultures-with survival of an e. coli o157:h7 (eco157:h7) inoculum in beef carcass intervention trials. survival of one lactic acid bacteria starter culture (bactoferm lhp dry [pediococcus acidilactici and pediococcus pentosaceus]), a five-isolate biotype i inoculum, and a five-isolate non-e. coli coliform inoculum, was compared wi ... | 2010 | 21067675 |
| the efficacy of lactic acid bacteria pediococcus acidilactici, lactose and formic acid as dietary supplements for turkeys. | a feeding trial was performed on 1400 big-6 turkey toms divided into experimental groups subject to the use of dietary supplements. the ain of this study was to evaluate the efficacy of the probiotic supplement bactocell, containing lactic acid bacteria pediococcus acidilactici, and lactose, administered to turkeys separately or in combination, as well as a formic acid supplement. the addition of the probiotic under test (lactic acid bacteria pediococcus acidilactici) to diets for turkeys contri ... | 2010 | 21077430 |
| optimized culture conditions for bacteriocin production by pediococcus acidilactici lab 5 and its characterization. | a strain of pediococcus acidilactici lab 5 was isolated from vacuum-packed fermented meat product, in order to obtain a novel bacteriocin from food-grade organisms. optimized culture conditions for bacteriocin production in different media (viz., mrs, tge, tge + buffer, tge + tween 80, and tge + tween 80 + buffer) and at different temperatures and ph conditions were reported. tge + tween 80 + buffer medium was found to be most effective for bacteriocin production (about 2400 au/ml) by this strai ... | 2008 | 21086723 |
| fusion expression of peda gene to obtain biologically active pediocin pa-1 in escherichia coli. | two heterologous expression systems using thioredoxin (trxa) as a gene fusion part in escherichia coli were developed to produce recombinant pediocin pa-1. pediocin pa-1 structural gene peda was isolated from pediococcus acidilactici pa003 by the method of polymerase chain reaction (pcr), then cloned into vector pet32a(+), and expressed as thioredoxin-peda fusion protein in the host strain e. coli bl21 (de3). the fusion protein was in the form of inclusion body and was refolded before purificati ... | 2011 | 21194188 |
| potential beneficial properties of bacteriocin-producing lactic acid bacteria isolated from smoked salmon. | aims: to evaluate the probiotic properties of strains isolated from smoked salmon and previously identified as bacteriocin producers. methods and results: strains lactobacillus curvatus et06, et30 and et31, lactobacillus fermentum et35, lactobacillus delbrueckii et32, pediococcus acidilactici et34 and enterococcus faecium et05, et12 and et88 survived conditions simulating the gastrointestinal tract (git) and produced bacteriocins active against several strains of listeria monocytogenes, but pr ... | 2011 | 21251174 |
| thermotolerant bacteriocin-producing lactic acid bacteria isolated from thai local fermented foods and their bacteriocin productivity. | twenty-one samples of thai local fermented foods were screened for thermotolerant bacteriocin-producing lactic acid bacteria. from 529 isolates of lactic acid bacteria, 121 isolates were able to inhibit the growth of certain bacterial strains. of these 121 isolates, only 11 produced antibacterial agents that were capable of inhibiting the growth of multiple bacterial strains in a liquid medium. one strain (kku 170) of these 11 isolates produced an antibacterial agent that could strongly inhibit ... | 2011 | 21467627 |
| application of lactobacillus fermentum hl57 and pediococcus acidilactici sp979 as potential probiotics in the manufacture of traditional iberian dry-fermented sausages. | the purpose of this study was to investigate the potential of implanting the probiotics lactobacillus fermentum hl57 and p. acidilactici sp979 during manufacture of iberian dry-fermented sausages, and their effect on the sensory properties of these meat products. microbial counts were studied in sausages inoculated with these strains and compared with a control batch. to detect the presence of probiotic strains at high counts (>7 log cfu/g) in the inoculated sausages, specific pcr and (rapd)-pcr ... | 2011 | 21569925 |
| exploitation of sweet cherry (prunus avium l.) puree added of stem infusion through fermentation by selected autochthonous lactic acid bacteria. | strains of lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus and leuconostoc mesenteroides subsp. mesenteroides were identified from 8 cultivars of sweet cherry by partial 16s rrna gene sequence and subjected to typing by random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) analysis. representative isolates from each species and each cultivar were screened based on the kinetics of growth on cherry puree added of (10%, v/v) stem infusion (cp-si). a protoc ... | 2011 | 21569932 |
| comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in ivory coast and brazil. | to compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one ivorian farm) and box (two brazilian farms) fermentations were carried out. all fermentations were studied through a multiphasic approach. in general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48°c. the main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mann ... | 2011 | 21569940 |
| administration of probiotics influences f4 (k88)-positive enterotoxigenic escherichia coli attachment and intestinal cytokine expression in weaned pigs. | abstract: this study evaluated the effect of the probiotics pediococcus acidilactici and saccharomyces cerevisiae boulardii on the intestinal colonization of o149 enterotoxigenic escherichia coli harbouring the f4 (k88) fimbriae (etec f4) and on the expression of ileal cytokines in weaned pigs. at birth, different litters of pigs were randomly assigned to one of the following treatments: 1) control without antibiotics or probiotics (ctrl); 2) reference group in which chlortetracycline and tiamul ... | 2011 | 21605377 |
| detection and characterization of pediocin pa-1/ach like bacteriocin producing lactic acid bacteria. | fifty-five bacteriocinogenic lactic acid bacteria (lab) isolated from seven different sources. eight isolates were found to produce pediocin pa-1 like bacteriocin as detected by pedb gene pcr and dot-blot hybridization. the culture filtrate (cf) activity of these isolates exhibited strong antilisterial, antibacterial activity against tested food-borne pathogens and lab. the identification and genetic diversity among the selected lab was performed by conventional morphological and molecular tools ... | 2011 | 21656248 |
| characterization of two distinct gh family 78 {alpha}-l-rhamnosidases from pediococcus acidilactici. | a-l-rhamnosidases play an important role in the hydrolysis of glycosylated aroma compounds (esp. terpenes) from wine. although several authors have demonstrated the enological importance of fungal rhamnosidases, the information on bacterial enzymes in this context is still limited. in order to fill this important gap, two putative rhamnosidase genes (ram, ram2) from pediococcus acidilactici dsm 20284 were heterologously expressed and the respective gene products characterized. in combination wit ... | 2011 | 21784921 |
| evolution of fermenting microbiota in tarhana produced under controlled technological conditions. | the purpose of this study was to evaluate the evolution of lactic acid bacteria (lab) and yeasts during the fermentation of tarhana produced with some pasteurised ingredients and carried out at 30 and 40-á-¦c. the chemical parameters were those typical for tarhana production. coliform bacteria were not detected during fermentation, while lab and yeasts were in the range 10(7)-10(8)-ácolony forming units (cfu)-ág(-1). plate counts showed an optimal development of both fermenting microbial groups ... | 2011 | 21839387 |
| protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin a in lactic acid bacteria. | replacement of the leader sequence (ls) of the bacteriocin enterocin a (ls(enta)) by the signal peptides (sp) of the protein usp45 (sp(usp45)), and the bacteriocins enterocin p (sp(entp)), and hiracin jm79 (sp(hirjm79)) permits the production, secretion, and functional expression of enta by different lactic acid bacteria (lab). chimeric genes encoding the sp(usp45), the sp(entp), and the sp(hirjm79) fused to mature enta plus the enta immunity genes (enta+entia) were cloned into the expression ve ... | 2011 | 21839785 |
| detection, cellular localization and antibacterial activity of two lytic enzymes of pediococcus acidilactici atcc 8042. | aim:ôçé in pediococcus acidilactici atcc 8042, two activities of peptidoglycan hydrolase (pgh) with lytic effect against micrococcus lysodeikticus and staphylococcus aureus have been detected. this work intends to elucidate the growth phase of maximum lytic activity, the localization and the effectiveness of the activity against pathogenic gram-negative and gram-positive bacteria. methods and results:ôçé cells were grown in mrs medium and collected at different growth stages, and the proteins we ... | 2011 | 21707881 |
| spoilage characteristics of traditionally packaged ground beef with added lactic acid bacteria displayed at abusive temperatures. | growth of pathogenic organisms such as escherichia coli o157:h7 and salmonella spp. can be inhibited in ground beef through the addition of certain lactic acid-producing bacteria (lab; lactobacillus acidophilus np51, lactobacillus crispatus np35, pediococcus acidilactici, and lactococcus lactis ssp. lactis). this study evaluated the effects of lab inclusion on the organoleptic and biochemical properties typically associated with spoilage in traditionally packaged ground beef displayed at abusive ... | 2011 | 22064744 |
| enhanced antibacterial activity of bimetallic gold-silver core-shell nanoparticles at low silver concentration. | herein we report the development of bimetallic au@ag core-shell nanoparticles (nps) where gold nanoparticles (au nps) served as the seeds for continuous deposition of silver atoms on its surface. the core-shell structure and morphology were examined by uv-vis spectroscopy, transmission electron microscopy (tem), energy dispersive x-ray (edx) analysis and x-ray diffraction (xrd). the core-shell nps showed antibacterial activity against both gram negative (escherichia coli and pseudomonas aerugino ... | 2011 | 22057130 |
| Role of an autochthonous starter culture and the protease EPg222 on the sensory and safety properties of a traditional Iberian dry-fermented sausage "salchichón". | The effect of the addition of an autochthonous starter culture and the protease EPg222 on the physico-chemical and sensory characteristics of dry-fermented sausage ''salchichon" was investigated. Sausages were prepared with purified EPg222 and Pediococcus acidilactici MS200 and Staphylococcus vitulus RS34 as starter culture (P200S34), separately and together, ripened for 90 days, and compared with a control batch. Dry-fermented sausages ripened with EPg222 and starter culture showed higher amoun ... | 2011 | 21925025 |
| screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausages. | the purpose of this study was to select lactic acid bacteria and bifibobacteria strains as potential probiotic cultures during the processing of iberian dry fermented sausages. a total of 1000 strains were isolated from iberian dry fermented sausages (363), and human (337) and pig faeces (300) in different culture media. around 30% of these strains, mainly isolated from iberian dry fermented sausages in lamvab agar, were pre-selected for testing as potential probiotics by their ability to grow a ... | 2008 | 22063588 |
| [Microbial diversity of a community for ensiling rice straw at low temperature and fermentation dynamics]. | To accelerate the conversion of rice straw into feeds in the low-temperature region, a microbial community was constructed by continuous enrichment cultivation. Microbial diversity and dynamics during the fermentation at 10 degrees C was analyzed. | 2011 | 22126081 |
| LysA2, the Lactobacillus casei bacteriophage A2 lysin is an endopeptidase active on a wide spectrum of lactic acid bacteria. | The lysin gene (lysA2) of the Lactobacillus casei bacteriophage A2 was cloned and expressed in Escherichia coli. LysA2 is an endopeptidase that hydrolyzes the bond between the terminal D: -alanine of the peptidoglycan tetrapeptide and the aspartic acid residue that forms the bridge with the L: -lysine of a neighboring peptidoglycan chain, characteristic of Gram-positive bacteria included into the A4 peptidoglycan subgroup. This includes most lactobacilli, Lactococcus lactis, Pediococcus acidilac ... | 2011 | 21952940 |
| effects of starter cultures on the formation of flavour compounds in dry sausage. | the purpose of this work was to study the impact of starter cultures on the production of flavour compounds in dry sausages. the effect of six starter cultures corresponding to different combinations of lactic acid bacteria (lactobacillus sake l110, pediococcus acidilactici 725, p. pentosaceus 716) and different staphylococcus species (s. carnosus 833, s. warneri 863, s. saprophyticus m31) strains were tested in a total of 30 dry sausages without spices. the analysis of flavour compounds using a ... | 1993 | 22061221 |
| Pediococcus acidilactici Endocarditis Successfully Treated with Daptomycin. | This report describes the first case of persistent bacteremia with endocarditis caused by Pediococcus acidilactici in a 32 year-old male with a history of short gut syndrome status post small bowel transplantation. The results showed the utility of sequencing the intergenic spacer region for species identification and successful treatment using daptomycin. | 2011 | 22205795 |
| lactic acid bacteria from chicken carcasses with inhibitory activity against salmonella spp. and listeria monocytogenes. | this study was conducted to isolate psychrotrophic lactic acid bacteria (lab) from chicken carcasses with inhibitory activity against strains of salmonella spp. and listeria monocytogenes. a total of 100 broiler samples were examined for the presence of lab. ninety-two lab isolates that showed antimicrobial effects against salmonella spp. and l. monocytogenes were further analysed to examine their lab (gram-positive, catalase negative, oxidase negative) and psychrotrophic characteristics (abilit ... | 2011 | 21983108 |
| the lactic acid bacterium pediococcus acidilactici suppresses autoimmune encephalomyelitis by inducing il-10-producing regulatory t cells. | certain intestinal microflora are thought to regulate the systemic immune response. lactic acid bacteria are one of the most studied bacteria in terms of their beneficial effects on health and autoimmune diseases; one of which is multiple sclerosis (ms) which affects the central nervous system. we investigated whether the lactic acid bacterium pediococcus acidilactici, which comprises human commensal bacteria, has beneficial effects on experimental autoimmune encephalomyelitis (eae), an animal m ... | 2011 | 22110705 |
| rapid differentiation of lactic acid bacteria from autochthonous fermentation of iberian dry-fermented sausages. | the populations of lactic acid bacteria (lab) in different types of iberian dry-fermented sausages from central-west spain were identified. a simple and rapid electrophoretic method of whole-cell protein profiles was evaluated, correlating it with 16s rrna gene sequence analysis and biochemical identification by api 50 chl. a total of 96 isolates were identified by sds-page showing stable profiles corresponding to 30-45 polypeptides in the range 95-8kda that were clearly different for the differ ... | 2008 | 22063578 |
| effect of a bacteriocin produced by pediococcus acidilactici against listeria monocytogenes and clostridium perfringens on spanish raw meat. | the inhibitory effect of a bacteriocin, produced by pediococcus acidilactici, against listeria monocytogenes and clostridium perfringens on spanish raw meat surface, was evaluated by in situ assays. samples were incubated with the bacteriocin and then with a culture of the pathogenic bacteria. the treatment with 500, 1000 or 5000 bacteriocin units/ml (bu/ml) reduced the counts of l. monocytogenes after storage at 15°c during 72h by 1, 2 or 3 log cycles and with 1000 or 5000 bu/ml after storage a ... | 2006 | 22061374 |
| bacteriocinogenic activity from starter cultures used in spanish meat industry. | thirty-nine bacterial strains, obtained from commercial starter cultures and commonly used by the meat industry in spain, have been examined for their ability to produce bacteriocins. fourteen (35%) of them showed antagonism against at least one of the indicator strains, by the agar spot test. the strains showing an inhibitory action against pathogenic indicator strains were identified as pediococcus acidilactici, lactobacillus curvatus, lactobacillus pentosus and lactobacillus plantarum, which ... | 2002 | 22061417 |
| screening of commercial meat starter cultures at low ph and in the presence of bile salts for potential probiotic use. | the survival of lactic acid bacterial strains from eight meat starter cultures under conditions similar to those in the gastrointestinal tract was determined. the conditions in stomach were simulated by using ph 1-5 phosphate-buffered saline. the conditions in small intestine were simulated by using mrs broth over a ph range 4-7 and two bile salt concentrations (0.15% and 0.30%). the survival capacity at ph 3 was strain dependent. 0.30% bile salts were critical for screening bile salt tolerant s ... | 2000 | 22061286 |
| Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. | To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We asse ... | 2012 | 21989255 |
| effects of starter cultures and nitrite levels on formation of biogenic amines in sucuk. | the effects of starter cultures, starter a (lactobacillus sakei+staphylococcus carnosus) and starter b (pediococcus acidilactici+staphylococcus xylosus+lactobacillus curvatus), nitrite levels (0, 75, and 150ppm) and ripening period on biogenic amine production were investigated in sucuk (turkish dry-fermented sausage) production. changes in biogenic amines, ph, water activity, moisture, non-protein nitrogen, nitrite and counts of lactic acid bacteria, micrococcus/staphylococcus, enterobacteriace ... | 2007 | 22061796 |
| modelling growth and bacteriocin production by pediococcus acidilactici pa003 as a function of temperature and ph value. | to investigate the effect of ph and temperature on the cell growth and bacteriocin production of pediococcus acidilactici pa003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of p. acidilactici pa003 was simulated in vitro during laboratory fermentations by making use of mrs broth. firstly, primary models were developed for cell growth, glucose consumption, lactic acid and bacteriocin production for a given set of environmental conditions. based on ... | 2012 | 22246730 |
| nutritional quality of fermented defatted soya and flaxseed flours and their effect on texture and sensory characteristics of wheat sourdough bread. | the use of soya and flaxseed flours fermented with pediococcus acidilactici for wheat sourdough bread production was investigated. the protein digestibility, biogenic amine contents of soya and flaxseed sourdoughs, texture and sensory features of bread were studied. the fermentation with p. acidilactici significantly improved soya and flaxseed protein extraction and increased protein digestibility on an average by 13.5%. the concentrations of histamine (3.8 ± 2.3 and 4.0 ± 0.2 mg/kg), tyramine ( ... | 2012 | 22229277 |
| draft genome sequence of probiotic strain pediococcus acidilactici ma18/5m. | pediococcus acidilactici ma18/5m is a commercially available probiotic that is widely used in swine, poultry, aquaculture feeds, and human dietary supplements. we prepared a genome sequence for this strain consisting of 2 scaffolds totaling 1,992,928 bases including gaps for a total of 3,346 bases and a g+c content of 42%. | 2012 | 22275099 |
| characterization of d: -lactate dehydrogenase from pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid. | the gene coding for d: -lactate dehydrogenase (d: -ldh) from pediococcus acidilactici dsm 20284 was cloned and expressed in e. coli. the recombinant enzyme was purified by nickel-affinity chromatography. it converted phenylpyruvic acid (ppa) to 3-phenyllactic acid maximally at 30°c and ph 5.5 with a specific activity of 140 and 422 u/mg for ppa and pyruvate, respectively. the k (m), turnover number (k (cat)), and catalytic efficiency (k ... | 2012 | 22261863 |
| identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage. | in order to understand the relationship between lactic acid bacteria (lab) species and silage fermentation, a total of 65 lab strains isolated from mixed pasture of timothy (phleum pratense l.) and orchardgrass (dactylis glomerata l.), and its badly preserved silages were subjected to phenotypic and genetic analysis. according to these analyses, the isolates were divided into 13 groups, including enterococcus gallinarum, lactobacillus acidipiscis, l. coryniformis subsp. coryniformis, l. corynifo ... | 2011 | 22515692 |
| fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives. | effects of lactic acid bacteria (lab) inoculants and cellulase additives on fermentation quality and chemical compositions of shrub silages were studied by using a small-scale fermentation system. two lab inoculants of qingbao (lactobacillus plantarum, pediococcus acidilacticii, lactobacillus casei and clostridium phage) and caihe (lactobacillus plantarum, lactobacillus brevis and pediococcus acidilactici) and a commercial cellulase made from trichoderma reesei were used as additives for interme ... | 2011 | 22515690 |
| impact of pre-selected autochthonous starter cultures on the flavor quality of iberian dry-fermented "salchichón" sausage with different ripening processes. | the purpose of this study was to investigate the impact on flavor quality of 2 autochthonous starter cultures in the manufacture of iberian dry-fermented "sálchichón." a total of 2 strains of pediococcus acidilactici (ms200 and ms198) were combined individually with a strain of staphylococcus vitulus (rs34) to obtain 2 starter cultures: p200s34 and p198s34. the ability of both starter cultures to implant during 2 different manufacturing procedures of "salchichón" was evaluated by 16s rrna gene s ... | 2011 | 22416726 |
| isolation and characterization of pediocin nv 5 producing pediococcus acidilactici lab 5 from vacuum-packed fermented meat product. | a potentially novel antimicrobial compound producing pediococcus acidilactici lab 5 was isolated from vacuum-packed fermented meat product. this compound was found active against some species of enterococcus, leuconostoc, staphylococcus and listeria, many of which are associated with food spoilage and food related health hazards. the strain was found to be a paired cocci which can utilize a broad range of carbohydrates and produce acid identical to the p. acidilactici and p. pentoseus. since the ... | 2011 | 22282624 |
| use of autochthonous pediococcus acidilactici and staphylococcus vitulus starter cultures in the production of "chorizo" in 2 different traditional industries. | the present study determined how the different ripening conditions affected the growth and development of 3 autochthonous starter cultures, and the physico-chemical and sensory characteristics of chorizo. each of 3 strains of pediococcus acidilactici (mc184, ms198, and ms200) and one of staphylococcus vitulus (rs34) were associated to prepare the starter cultures, p184s34, p198s34, and p200s34. then, chorizo was prepared following 2 manufacturing procedures. the autochthonous starter cultures we ... | 2011 | 22260118 |
| calculation of substrate binding affinities for a bacterial gh78 rhamnosidase through molecular dynamics simulations. | ram2 from pediococcus acidilactici is a rhamnosidase from the glycoside hydrolase family 78. it shows remarkable selectivity for rutinose rather than para-nitrophenyl-alpha-l-rhamnopyranoside (p-npr). molecular dynamics simulations were performed using a homology model of this enzyme, in complex with both substrates. free energy calculations lead to predicted binding affinities of -34.4 and -30.6 kj mol(-1) respectively, agreeing well with an experimentally estimated relative free energy of 5.4 ... | 2013 | 23914137 |
| gut hormones in fetal distress. | | 1999 | 91067 |
| urinary diversion: a new angle? | | 2012 | 3671701 |
| the use of affinity chromatography for the specific purification of antibodies and antigens. | | 2014 | 4184826 |
| tuberous sclerosis and schizophrenia. | | 2015 | 4648445 |
| [thrombotic occlusion and late lysis in the arteria subclavia lusoria dextra]. | case history of a 45 years aged male patient with an aortic arch anomaly. thrombotic occlusion of the a. lusoria dextra with symptoms of a subclavian-steal syndrome and anoxia in the right hand. spontaneous lysis after 8 weeks is documentated by arteriography. | 2005 | 1183296 |
| new jersey aids patients often drug abusers. | | 2012 | 3457361 |
| biomedical librarians in a patient care setting at the university of missouri-kansas city school of medicine. | the biomedical librarian has been placed in a patient care setting working in the specific environment of a six-year medical school guided by the docent team concept (docent is defined as a clinician-scholar). within this framework the specifically qualified clinical medical librarians function within the docent unit. three dissemination techniques or systems-latch, current references, and latest topics-are discussed. note is made of the clinical medical librarian's role in teaching, demand sear ... | 2015 | 4462680 |
| residual effectiveness of insecticides for control of german cockroaches (dictyoptera: blattellidae) in food-handling establishments. | residual effectiveness of insecticides was compared for two treatment methods at various sites within two commercial kitchens. for all sites and insecticides, german cockroach, blattella germanica (l.), mortality was greatest for treatments of cracks and crevices than for treatments of exposed surfaces. mortality at the dishwashing site was lower than at all other sites. we attributed reduced mortality at the dishwashing site to a higher vapor pressure deficit. mortality and the amount of insect ... | 2007 | 2258513 |
| appendicitis in pregnancy. | | 2010 | 2868213 |
| omega 3-fatty acids in health and disease. | | 2011 | 3067452 |
| determinants of mortality in pediatric patients with greater than 70% full-thickness total body surface area thermal injury treated by early total excision and grafting. | recent advancements in burn care have improved the survival rates of victims with severe burn injuries. the total mortality rate in a series of 1,057 pediatric patients admitted to shriners burns institute galveston unit between 1982 and 1986 was 2.7%. the burn size resulting in a 50% death rate was 95% of the total body surface area (tbsa). in this study 19 survivors and 13 nonsurvivors with greater than 70% full-thickness tbsa burn injuries were compared. all survivors were adequately resuscit ... | 2012 | 3546714 |
| [an experimental study on srca (subrenal capsule assay)--comparison of the test between normal and nude mice]. | six-day srca using normal mice developed by bogden et al. is a promising in vivo chemosensitivity test. however, this method has a problem on the influence of the host reaction. we compared the tumor growth kinetics and host reaction between normal and nude mice. the tumor diameter increased until day 6 in normal and day 16 in nude mice, but the histological finding revealed many host reactive cells and few viable tumour cells on day 6 in normal mice, and well preserved tumour cells on day 16 in ... | 2012 | 3348635 |