| effect of reduction of the reaction center intermediate upon the picosecond oxidation reaction of the bacteriochlorophyll dimer in chromatium vinosum and rhodo pseudomonas viridis. | | 1977 | 880297 |
| influence of the chlorophylls on the electrochromism of carotenoids in the membranes of photosynthesis. | | 1977 | 891967 |
| possible initial events of photophosphorylation in membranes of rhodopseudomonas viridis and rhodopseudomonas capsulata ala+r. | | 1977 | 891968 |
| a role for ubiquinone-10 in the b--c2 segment of the photosynthetic bacterial electron transport chain. | | 1977 | 891997 |
| growth of rhodopseudomonas capsulata under anaerobic dark conditions with dimethyl sulfoxide. | | 1977 | 900930 |
| thermodynamics and kinetics of photophosphorylation in bacterial chromatophores and their relation with the transmembrane electrochemical potential difference of protons. | | 1977 | 913405 |
| [comparative study of light-harvesting complexes of purple photosynthetic bacteria chromatium minutissimum and rhodopseudomonas palustris]. | light-harvesting pigment-lipoprotein complexes from sulfur (chromatium minutissimum) and non-sulfur (rhodopseudomonas palustris) purple bacteria are isolated and comparatively studied. electron microscopy was used for determination of the complex size, different methods were employed to estimate their molecular weights and chemical composition. two small proteins are found in each complex, their molecular weight, molar ratio and their content per complex being studied. amino acid composition and ... | 1977 | 922068 |
| purification and properties of citrate lyase ligase from streptococcus diacetilactis. | citrate lyase ligase (acetate: sh--[acyl-carrier protein] enzyme ligase (amp) from streptococcus diacetilactis was purified 920-fold with a yield of 6.3%. the molecular weight of the enzyme was estimated to be 41000; the ligase consisted of one polypeptide chain. the acetylation of 1 mol of deacetyl-citrate lyase to enzymatically active citrate lyase required 6 mol atp. the formation of amp and pyrophosphate in the acetylation reaction was demonstrated. citrate lyase ligase was specific for the ... | 1977 | 923578 |
| hydrogen recycling by rhodopseudomonas capsulata. | | 1977 | 923798 |
| synchronous growth of rhodopseudomonas palustris from the swarmer phase. | rhodopseudomonas palustris was chosen as a model organism for studying bacterial differentiation. synchronous populations selected by sucrose gradient centrifugation yielded more than 95% swarmer cells. the appearance and disappearance of cell morphological groupings and the doubling of cell numbers in cultures of such swarmer populations were very well defined. cells were only motile for the first half of the division cycle, but motility was regained before division. development gave rise to a ... | 1976 | 932685 |
| [fractionation of carbon isotopes by photoautotrophic microorganism with different carbon dioxide assimilation pathways]. | | 1976 | 939167 |
| [dark metabolism of rhodospeudomonas sulfidophila]. | a new strain of phototrophic purple bacterium rhodopseudomonas sulfidophila rp-6 has been isolated from samples of the salty meromictic lake repnoye. the bacterium utilizes thiosulphate yielding sulphates and assimilates molecular hydrogen in the darkness under aerobic conditions. thiosulphate stimulates fixation of 14c-bicarbonate by the cells both in the light and darkness. organic compounds are necessary for growth of the bacterium in the darkness. r. sulphidophila rp-6, r. palustus, and rhod ... | 1976 | 940488 |
| metabolism of methanol by rhodopseudomonas acidophila. | rhodopseudomonas acidophila strain 10050, grown anaerobically in the light on methanol, contained a methanol and formaldehyde dehydrogenase which could be coupled to phenazine methosulphate; an nad-linked formaldehyde dehydrogenase which required gsh for activity; and an nad-linked formate dehydrogenase. the specific activities of these enzymes varied in a non-coordinate manner when the organism was grown on different alcohols, formate or succinate. the affinity of the phenazine methosulphate li ... | 1976 | 950554 |
| cysteine and s-sulfocysteine biosynthesis in phototrophic bacteria. | forteen species (17 strains) of phototrophic bacteria as well as one strain of thiobacillus denitrificans were tested for cysteine synthase and s-sulfocysteine synthase. all strains contain cysteine sythase active with o-acetylserine; only the chromatiaceae, two species of the rhodospirillaceae and t. denitrificans contain s-sulfocysteine synthase. in six species repression by different sulfur compounds in the medium was studied. in chromatium vinosum, cysteine synthase was found to be constitut ... | 1976 | 962465 |
| genetic transfer of the capacity to form bacteriochlorophyll-protein complexes in rhodopseudomonas capsulata. | | 1976 | 964375 |
| isolation and characterization of a glycerol auxotroph of rhodopseudomonas capsulata: effect of lipid synthesis on the synthesis of photosynthetic pigments. | a glycerol auxotroph was isolated from rhodopseudomonas capsulata for use as a system for studying membrane synthesis and function. when the mutant was deprived of glycerol, net phospholipid synthesis ceased immediately and a small amount of free fatty acids accumulated. a turnover of lipid occurred in both deprived and supplemented cultures. deoxyribonucleic acid and protein synthesis continued for one doubling of cell massand then slowed down in deprived cells. net ribonucleic acid synthesis s ... | 1976 | 977539 |
| photooxidation of p-960 and photoreduction of p-800 (bacteriopheophytin b-800) in reaction centers from rhodopseudomonas viridis. | the light excitation of p-960 results in the oxidation of p-960 and the reduction of p-800 (bacteriophytin b-800) in the reaction centers from rhodopseudomonas viridis. a negative 847 nm band of the circular dichroism psectrum disappears under p-960 photooxidation, while a positive 827 nm band disappears under p-800 photoreduction. exciton interaction of the pigment molecules in the reaction center is discussed. | 1976 | 999857 |
| characterization of a diaminohexose (2,3-diamino-2,3-dideoxy-d-glucose) from rhodopseudomonas viridis lipopolysaccharides by circular dichroism. | | 1976 | 1000526 |
| microbial catabolism: its role in the carbon cycle and environmental significance: a colloquium organized by p. a. williams (bangor). | | 1976 | 1001697 |
| anaerobic photometabolism of aromatic compounds by rhodopseudomonas sp. | | 1976 | 1001709 |
| [utilization of nitrogen compounds by phototrophic bacteria]. | phototrophic purple and green bacteria differ by their ability to assimilate nitrogen compounds. thiocapsa roseopersicina utilizes not only ammonium and nitrates, as a source of nitrogen, but also urea, azoguanine, cytosine and some amino acids. the non-sulphur bacterium rhodopseudomonas palustris grows at the account of amine nitrogen of a larger number of amino acids than the sulphur bacterium. chlorobium limicola 1. thiosulfatophilum grows only on media containing urea and methylamines. forma ... | 1976 | 1004251 |
| [morphological differentiation in non-sulfur purple bacteria]. | cell differentiation in non-sulphur purple bacteria is complicated, as compared to binary fission, during bud formation with production of hyphae and special resting cells of the exospore type, and can be demonstrated in the folowing series of microorganisms: rhodopseudomonas sulfidophila leads to rh. capsulata leads to rh. acidophila leads to rh. viridis and rh. palustris leads to rh. sulfoviridis leads to rhodom. vannielii. contrary to phototrophic bacteria multiplying by division, a distinct ... | 1976 | 1004269 |
| acetate metabolism in rhodopseudomonas gelatinosa and several other rhodospirillaceae. | when rhodopseudomonas gelatinosa was grown on acetate aerobically in the dark both enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase, could be detected. however, under anaerobic conditions in the light only isocitrate lyase, but not malate synthase, could be found. the reactions, which bypass the malate synthase reaction are those catalyzed by alanine glyoxylate aminotransferase and the enzymes of the serine pathway. other rhodospirillaceae were tested for isocitrate lyase a ... | 1976 | 1015959 |
| [bacteriochlorophyll fluorescence changes related to the bacteriopheophytin photoreduction in the chromatophores of purple sulfur bacteria]. | it is shown that illumination of chromatophores of sulfur bacterium chromatium minutissimum at eh of the medium --200 mv divided by --620 mv (when the photooxidation of pigment p890 is completely inhibited) induces a decrease in bacteriochlorophyll fluorescence yield, reversible in the dark. under these conditions a reversible photoreduction of bacteriopheophytin is detected (bleaching of absorption bands at 543 and 760 nm and development of a band at 650 nm), which is accompanied by a blue shif ... | 1976 | 1024595 |
| possible mechanism of photophosphorylation in rhodopseudomonas viridis. | photophosphorylation in the presence of (32p) inorganic phosphate (pi) and adp or pi and (beta 32p) -adp was carried out in membranes of rhodopseudomonas viridis. when pi and (32p)-adp were used, 32pi and (32p)-atp were detected by ion exchange chromatography and the specific activity of the (beta 32p)-adp recovered was substantially diminished. moreover, when the (32p)-atp was analyzed, the radioactivity was equally distributed between its beta and gamma position. when 32pi and adp were used in ... | 1976 | 1052599 |
| anaerobic growth of a rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. | a species of rhodopseudomonas that grows under strict anaerobic conditions in the dark and requires co was isolated from lake and pond sediments. although anaerobic growth in the dark occurs in a chemically defined mineral medium with co as the only carbon and energy source, growth is stimulated by adding trypticase. under these conditions, cells exhibit a generation time of 6.7 hr and reach a final concentration of 1 to 3 x 10(9) cells per ml of liquid medium. resting suspensions of co-grown ce ... | 1976 | 1067620 |
| studies of pigments and lipids in rhodopseudomonas spheroides y reaction center. | | 1975 | 1079006 |
| excited states of photosynthetic reaction centers at low recox potentials. | in preparations of photochemical reaction centers from rhodopseudomonas spheroides r-26, lowering the recox potential so as to reduce the primary electron acceptor prevents the photochemical transfer of an electron from bacteriochlorophyll to the acceptor. measuring absorbance changes under these conditions, we found that a 20-ns actinic flash converts the reaction center to a new state, p-f, which then decays with a half-time that is between 1 and 10 ns at 295 degrees k. at 25 degrees k, the ... | 1975 | 1079143 |
| picosecond detection of an intermediate in the photochemical reaction of bacterial photosynthesis. | preparations of photosynthetic reaction centers from rhodopseudomonas sphaeroides were excited with flashes lasting approximately 8 psec. immediately after the excitation, there appeared a transient state which was characterized by new absorption bands near 500 and 680 nm, by a bleaching of bands near 540, 600, 760, and 870 nm, and by a blue shift of a band near 800 nm. the transient state decayed with an exponential decay time,t, of 246 plus or minus 16 psec after the flash. as the transient st ... | 1975 | 1079602 |
| porphyrin synthesis: some particular approaches. | | 1975 | 1079708 |
| regulation of c3-enzymes in facultative phototrophic bacteria: the cold-labile pyruvate kinase of rhodopseudomonas sphaeroides. | pyruvate kinase (ec2.7.1.40) from rhodopseudomonas sphaeroides was purified 40-fold be precipitation with protamine sulfate and ammonium sulfate followed by gel-filtration. the preparations obtained from cells grown with different carbon sources or cultural conditions differ with respect to specific activity but not with respect to molecular weight (250000 dalton) or regulatory properties. the phosphoenolpyruvate (pep)-saturation cruve of the enzyme is sigmoidal with hill coefficients varying fr ... | 1975 | 1080043 |
| a possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of rhodopseudomonas sphaeroides. | a previously uncharacterized bacteriochlorophyll-less mutant (mutant 8) of rhodopseudomonas sphaeroides has been found to excrete a tetrapyrrole-protein complex into the incubation medium. the structure of the major pigment of the complex was characterized as 2-desacetyl-2-vinylbacteriopheophorbide. the corresponding magnesium derivative does not fit into the currently proposed biosynthetic pathway for bacteriochlorophyll, and thus may indicate the existence of an alternate pathway of bacterioch ... | 1975 | 1080053 |
| detection of two further beta-type cytochromes in rhodopseudomonas spheroides. | the photosynthetically-incompetent mutant v-2 of rhodopseudomonas spheroides which is incapable of synthesising bacteriochlorophyll was grown aerobically under conditions of both high and low aeration. potentiometric titration a at 560 nm minus 570 nm revealed the presence of several different components tentatively identified as b-type cytochromes. two such components of oxidation-reduction midpoint potentials of +390 mv +/- 10 mv and +255 mv +/- 7mv have not previously been detected in membran ... | 1975 | 1080425 |
| the functional unit of electrical events and phosphorylation in chromatophores from rhodopseudomonas sphaeroides. | 1. from electron micrographs of chromatophores from rhodopseudomonas spaeroides and from the estimated bacteriochlorophyll per chromatophore was estimated. the mean diameter of the chromatophore vesicles was 600 a. 2. the decay of the flash-induced electric potential across the chromatophore membrane measured by the carotenoid band shift was 20% accelerated by about one valinomycin molecule per 4700 bacteriochlorophyll, i.e. by one ionophore molecule per chromatophore. 3. the inhibition of the f ... | 1975 | 1080674 |
| control of 5-aminolaevulinate synthetase activity in rhodopseudomonas spheroides a role for trisulphides. | 1. the aminolaevulinate synthetase activator of fresh extracts of semi-anaerobically grown rhodopseudomonas spheroids was resolved into two fractions by ion-exchange chromatography. one fraction was identified as cystine trisulphide (cyssscy). analysis of the other fraction indicated the presence of about equal amounts of glutathione trisulphide (gsssg) and the mixed trisulphide of glutathione and cystine (gssscy). 2. four further fractions with activator activity were observed on ion-exchange c ... | 1975 | 1080999 |
| primary acceptor in bacterial photosynthesis: obligatory role of ubiquinone in photoactive reaction centers of rhodopseudomonas spheroides. | reaction centers were found to bind two ubiquinones, both of which could be removed by o-phenanthroline and the detergent lauryldimethylamine oxide. one ubiquinone was more easily removed than the other. the low-temperature light-induced optical and electron paramagnetic resonance (epr) changes were eliminated and restored upon removal and readdition of ubiquinone and were quantitatively correlated with the amount of tightly bound ubiquinone. we, therefore, conclude that this ubiquinone plays a ... | 1975 | 1081231 |
| cytoplasmic and outer membranes separation in rhodopseudomonas sphaeroides. | a cell envelope fraction had been prepared after mechanical disruption of lysozyme-edta spheroplasts from depigmented rhodopseudomonas sphaeroides (aerobically grown in the light). on linear sucrose gradients this fraction can be separated in a cytoplasmic membrane fraction and an outer membrane fraction. the cytoplasmic fraction (buoyant density: 1.18 g/cm3) has been characterized by its succinic dehydrogenase activity and by its composition. the outer membrane fraction (buoyant density: 1.21 g ... | 1975 | 1081384 |
| transport of amino acids in membrane vesicles of rhodopseudomonas spheroides energized by respiratory and cyclic electron flow. | active transport of amino acids in whole cells and membrane vesicles from the facultative photo-synthetic bacterium rhodopseduomonas spheroides is coupled to electron flow in the respiratory chain and in the cyclic electron transfer system. in vesicles made from cells grown aerobically in the dark transport of amino acids is most effectively energized by the oxidation of nadh and to a lesser extent by ascorbate or succinate in the presence of n,n,n',n'-tetramethyl-1, 4-phenyldiamine dihydrochlor ... | 1975 | 1081452 |
| glycerol dissimilation in rhodopseudomonas sphaeroides. | rhodopseudomonas sphaeroides followed a diauxic growth curve when grown on a malate-glycerol medium, the first phase of growth being supported by malate and the second by glycerol. a soluble glycerokinase and a particulate, pyridine nucleotide-independent glycerophosphate dehydrogenase, were induced by the presence of glycerol in the medium, but neither was fully expressed nor functional until all malate had been consumed. | 1975 | 1081535 |
| the nature of a pgiment-protein complex excreted from mutants of rhodopseudomonas sphaeroides. | pigment-protein complexes excreted from three bacteriochlorophyll-less mutants (mutants 8, 8-29, and 8-47) of rhodopseudomonas sphaeroides have been isolated and purified. in the absence of detergents the complexes remained in an aggregated state, but were disaggregated by 0.2% triron x-100. sepharose 6b gel filtration indicated that the disaggregated complex from each of the mutants had a particle weight of about 165000, and contained 30 +/- 3% protein. this complex was further dissociated by 1 ... | 1975 | 1081882 |
| 5-aminolevulinic-acid synthetases from rhodopseudomonas spheroides y. comparison of the purification and properties of enzymes extracted from bacteria grown in different iron concentrations. | the two 5-aminolevulinic acid synthetases of rhodopseudomonas spheroides y. were extracted from cells grown in a 'low-iron' medium and purified. they have a specific activity 10-fold higher than the 'high-iron' enzymes described by us previously and have the same properties except that they do not contain any iron and have one free-sh group more per mole of enzyme (2 for e1; , for e2) their inhibition by adenosine triphosphate and iron and their oxidation-reduction sensitivity are discussed in t ... | 1975 | 1081946 |
| extrachromosomal deoxyribonucleic acid in wild-type and photosynthetically incompetent strains of rhodopseudomonas spheroides. | three covalently closed circular species of extrachromosomal deoxyribonucleic acid have been identified by electron microscopic analysis in strains of rhodopseudomonas spheroides. the weights of these plasmids, as determined from contour length, are about 75 x 10(6), 66 x 10(6), and 28 x 10(6) daltons for both aerobically grown and photosynthetically grown r. spheroides strain 2.4.1 (nrs) and for the photosynthetically incompetent strain v-2 (obtained by n-methyl-n-nitro-n'nitrosoguanidine mutag ... | 1976 | 1082886 |
| the type, amount, location, and energy transfer properties of the carotenoid in reaction centers from rhodopseudomonas sphaeroides. | analysis of photosynthetic reaction centers from rhodopseudomonas sphaeroides strains 2.4.1 and ga shows that each contains approx. 1 mol of a specific carotenoid per mol of reaction center. in strain 2.4.1. the carotenoid is spheroidene (1-methoxy-3,4-didehydro-1,2,7',8',-tetrahydro-psi,psi-carotene); in strain ga, it is chloroxanthin (1-hydroxy-1, 2, 7', 8'-tetrahydro-psi,psi-carotene). the carotenoid is bound to the same pair of proteins as are the bacteriochlorophylls and bacteriopheophytins ... | 1976 | 1083252 |
| resonance raman scattering of bacteriochlorophyll, bacteriopheophytin and spheroidene in reaction centers of rhodopseudomonas speroides. | | 1976 | 1083733 |
| [temperature dependence of the kinetics of dark reduction of bacteriochlorophyll p870, oxidized by impulse laser and continuous light in photosynthetic reaction center preparations from rhodosuedomonas spheroides strain 1760-1]. | a comparative study was carried out of temperature dependence of kinetics of dark reduction of bacteriochlorophyll p870 oxidized both by pulsed laser and continuous actinic light in preparations of photosynthetic reaction centres-bacteriochlorophyll-protein complexes from rhodopseudomonas spheroides, strain 1760-1. half-time of the recombination of primary products--p870+ and reduced primary electron acceptor a1, which decreases with temperature lowering from 180-240 ms at 120k, is determined. v ... | 1976 | 1083747 |
| separation of inner and outer membranes of rhodopseudomonas spheroides. | the separation of inner and outer membrane of rhodopseudomonas spheroides has been achieved by means of sucrose density gradient (20%, 40%, 60%, w/w) centrifugation. the upper fraction of the gradient, with a specific density 1.181 (g/cm3), is high in cytochrome and succinate dehydrogenase activities, low in lipopolysaccharides and it is designated the inner membrane fraction. the bottom fraction of the gradient, with a specific density 1.240, is high in lipopolysaccharide and contains neither c ... | 1976 | 1083979 |
| a 4-vinylprotochlorophyllide complex accumulated by "phofil" mutant of rhodopseudomonas spheroides. an authentic intermediate in the development of the photosynthetic apparatus. | a photosynthetically competent mutant strain of rhodopseudomonas spheroides was isolated. in addition to bacteriochlorophyll, this organism produced particle-bound precursor 4-vinylprotochlorophyllide. the spectral characteristics of the pigment complexes(es) accumulated in the culture medium were very variable. the spectral form occurring within the bacteria was characterized from fluorescence data. its particle weight, 130 000, was determined by sephadex g200 filtration. the main components of ... | 1976 | 1084160 |
| light-induced tetracycline accumulation by rhodopseudomonas sphaeroides. | light has been used as a primary energy source in studies of tetracycline transport by rhodopseudomonas sphaeroides. accumulation of the antibiotic occurs in light, while efflux occurs in dark. both fluorescence enhancement and radioisotopic tracing have been used to monitor transport. km's obtained from both techniques are similar. light-induced accumulation of tetracyclines is inhibited by a variety of inhibitors, including antimycin a, n-ethylmaleimide, carbonylcyanide m-chlorophenylhydrazone ... | 1976 | 1084445 |
| delayed fluorescence from rhodopseudomonas sphaeroides following single flashes. | delayed fluorescence from rhodopseudomonas sphaeroides chromatophores was studied with the use of short flashes for excitation. although the delayed fluorescence probably arises from a back-reaction between the oxidized reaction center bacteriochlorophyll complex (p+) and the reduced electron acceptor (x-), the decay of delayed fluorescence after a flash is much faster (tau1/2 approximately 120 mus) than the decay of p+x-. the rapid decay of delayed fluorescence is not due to the uptake of a pro ... | 1976 | 1084763 |
| rhodopseudomonas sphaeroides forma sp. denitrificans, a denitrifying strain as a subspecies of rhodopseudomonas sphaeroides. | from polluted water of a lagoon pond a new type of denitrifying photosynthetic purple bacteria was isolated. with respect to morphology, fine structure, photopigments, requirement for growth factors, the range of utilization of organic substrates for phototrophic growth and dna base ratio, the denitrifying strains show the closest resemblance to rhodopseudomonas sphaeroides and were therefore described as a subspecies named r. sphaeroides forma sp. denitrificans. the new isolates grow well with ... | 1976 | 1085137 |
| purification and physicochemical properties of superoxide dismutase from two photosynthetic microorganisms. | superoxide dismutase (ec 1.15.1.1) has been isolated and characterised from the blue-green alga spirulina platensis and from aerobically-grown rhodopseudomonas spheroides, a purple, non-sulphur bacterium. the former enzyme contains 1 gatom of iron and the latter 1 gatom of manganese per mol; both enzymes have a molecular weight of 37 000-38 000, being composed of two non-covalently joined subunits of equal size. various spectral studies have been carried out including absorbance, circular dichro ... | 1976 | 1085167 |
| membranes of rhodopseudomonas sphaeroides. iv. assembly of chromatophores in low-aeration cell suspensions. | chromatophore membrane formation was induced in low-aeration suspensions of rhodopseudomonas sphaeroides and highly purified chromatophore preparations were isolated at various intervals between 4 and 18 h. the levels of several functional components associated with the isolated strucures were investigated. b-875, the light-harvesting bacteriochlorophyll complex associated with the reaction center, was preferentially inserted into the chromatophore membrane during the early stages of induction, ... | 1976 | 1085168 |
| reactivation by glycerol and ethylene glycol of inactivated delta-aminolevulinic acid synthetase of rhodopseudomonas spheroides. | reactivation effects by glycerol and ethylene glycol of inactivated ala synthetase of r. spheroids were observed. accompanying the reactivation of the inactivated enzyme, km value for plp decreased to levels similar to those of the freshly prepared enzyme. | 1976 | 1085260 |
| incorporation of tritium into cell materials of rhodopseudomonas spheroides from tritiated water in the medium under aerobic conditions. | when rhodopseudomonas spheroides cells grown aerobically in the dark were incubated in medium containing tritiated water (tho), incorporation of t into the bacterial cell materials occurred under growth and no-growth conditions. the overall t incorporation under no-growth conditions was stimulated by vigorous aeration and was suppressed strongly in the presence of either 10(-3) m kcn or 0.3% hgcl2, indicating that the bulk of the incorporation might depend upon bacterial cell metabolism or respi ... | 1976 | 1086304 |
| [light-dependent tetracycline transport in rhodopseudomonas sphaeroides (proceedings)]. | | 1976 | 1086561 |
| changes of in vivo bacteriochlorophyll fluorescence yield in rhodopseudomonas sphaeroides at low temperature and low redox potential. | | 1976 | 1086798 |
| orientation of chromophores in reaction centers of rhodopseudomonas sphaeroides. evidence for two absorption bands of the dimeric primary electron donor. | chromatophores from rhodopseudomonas sphaeroides were oriented by allowing aqueous suspensions to dry on glass plates. orientation of reaction center pigments was investigated by studying the linear dichroism of chromatophores in which the absorption by antenna bacteriochlorophyll had been attenuated through selective oxidation. alternatively the light-induced absorbance changes, in the ranges 550-650 and 700-950nm, were studied in untreated chromatophores. the long wave transition moment of rea ... | 1976 | 1087160 |
| a partial reaction of delta-aminolaevulinate synthetase from rhodopseudomonas spheroides. | | 1976 | 1087256 |
| effect of added light-harvesting pigments on the reconstruction of photosynthetic reactions in membranes from bacteriochlorophyll-less mutants of rhodopseudomonas sphaeroides. | | 1976 | 1087259 |
| the preparation of coproporphyrin iii and its iron complex from rhodopseudomonas spheroides. | | 1976 | 1088132 |
| [isolation and characterization of photochemical properties of the photosynthetic reaction centers from rhodopseudomonas shperoides, strain 1760-1]. | photosynthetic reaction centres were isolated from the cells of rhodopseudomonas spheroides, strain 1760-1, using sodium dodecyl sulphate. the preparations purified by precipitation with ammonium sulphate showed absorbance ratios of a280 : a800=2.1. and a765 : a800 : a870=1 : 2 : 1; about 75% of the bacteriochlorophyll absorbing at 870 nm (p870) were photochemically active. both absolute and difference "light minus dark" absorption spectra were obtained for the reaction centre suspensions and va ... | 1976 | 1088798 |
| reaction centers from rhodopseudomonas sphaeroides. | | 1976 | 1088799 |
| an anaerobic bag for photoheterotrophic growth of some rhodospirillaceae in petri dishes. | | 1975 | 1092643 |
| multiple-variant design for the enrichment of photosynthetic bacterial populations. | a design for the simultaneous variation of acetate, sulfide, and sulfate concentrations in 11 related media is described. selectivity of these media by a direct enumeration technique in solid culture was compared with that for enrichment in liquid culture. variation of nutritional parameters resulted in the selection of rhodospirillaceae at initial enrichment concentrations of 0.1 g or less of na2s-9 h2o per litre and chromatiaceae at 0.1 to 1.0 g of g of na2s-9 h2o per litre. the survival of ... | 1975 | 1097075 |
| characterization of rhodopseudomonas capsulata. | thirty-three strains of rhodopseudomonas capsulata have been studied in order to develop a more comprehensive characterization of the species. on the basis of morphological, nutritional, physiological and other properties, the characteristics of an "ideal biotype" have been defined, which can be used to distinguish rps. capsulata from similar purple bacteria. in this connection, two properties of rps. capsulata are of particular note: a) sensitivity to penicillin g is 10(3)-10(5) times greater t ... | 1975 | 1103769 |
| structural changes in the ribosomes and ribosomal proteins of rhodopseudomonas palustris. | | 1975 | 1111523 |
| direct observation of the rotation in a constant magnetic field of highly organized lamellar structures. | | 1975 | 1112417 |
| citrate lyase from streptococcus diacetilactis. association with its acetylating enzyme. | citrate lyase (ec 4.1.3.6) was purified 38-fold from cell-free extracts of streptococcus diacetilactis. the enzyme was homogeneous in analytical ultracentrifugation and polyacrylamide gel electrophoresis. the final enzyme preparation contained acetate: hs-citrate lyase ligase--an acetylating enzyme which converts inactive hs-citrate lyase into enzymatically active acetyl-s-citrate lyase. this enzyme activity was purified 25-fold over the crude extract and seemed to be associated with citrate lya ... | 1975 | 1115558 |
| magnesium 2,4-divinylphaeoporphyrin alpha-5 as a substrate for chlorophyll biosynthesis in vitro. | | 1975 | 1116606 |
| electron transport in an in vitro-reconstituted bacterial photophosphorylating system. | photooxidation of endogenous cytochrome(s) c, photoreduction of endogenous chrome(s) b and photobleaching of bacteriochlorophyll have been demonstrated in an in vitro reconstituted system, previously demonstrated to support photophosphorylation. the kinetic responses of these redox reactions to substrate and antimycin a in these particle are characteristic of electron transport processes and stronglysupport the contention that all, or a part of, the oxidative phosphorylation electron transport p ... | 1975 | 1125283 |
| a large photoreactive particle from chromatium vinosum chromatophores. | large photoreactive particles from chromatium vinosum are obtained pure and in high yield by using a mixture of detergents at high ionic strength to dissociate the chromatophore membrane. the particles contain all of the secondary electron acceptor of the chromatophores and about half of the cytochrome. their content of ubiquinone is greatly enridhed as compared with chromatophores. th individual particles have an estimated molecular weight of between 650,000 and 810,000. gel electrophoresis ... | 1975 | 1125294 |
| identification of a 2, 3-diamino-2, 3-dideoxyhexose in the lipid a component of lipopolysaccharides of rhodopseudomonas viridis and rhodopseudomonas palustris. | a hitherto unknown amino sugar (compound a), detected in acid hydrolyzates of lipopolysaccharides of rhodopseudomonas viridis and rhodopseudomonas palustris, is present in the lipid a component but not in the o-specific part of the lipopolysaccharides. 2-amino-2-deoxy-d-glucose is lacking in the purified lipid a of both strains. compound a, characterized by a very high migration in paper electrophoresis was obtained in a pure state by ion-exchange chromatography and shown by m.s. of the alditol ... | 1975 | 1125951 |
| growth inhibition of rhodopseudomonas capsulata by methylmercury acetate. | growth of rhodopseudomonas capsulata was inhibited in a bacteriostatic manner by as little as 10-minus 8 m methylmercury acetate (mehgac) in unsupplemented synthetic liquid medium or when cells were exposed to 8.0 nm of mehgac per mg of cell protein in a single exposure. | 1975 | 1147613 |
| lipophilic o-antigens containing d-glycero-d-mannoheptose as the sole neutral sugar in rhodopseudomonas gelatinosa. | lipopolysaccharides (lps, o-antigens) of 12 strains of the photosynthetic bacterium rhodopseudomonas gelatinosa were obtained by the phenol/chloroform/petroleum ether method, recommended for extracting lipophilic glycolipids of enterobacterial r-mutants. all r. gelatinosa lps have essentially the same chemical composition. similar to lps of salmonella r-mutants of chemotypes rd1 and rd2, the sole neutral sugar constituent is an aldoheptose. the heptose of r. gelatinosa lps has the d-glycero-d-ma ... | 1975 | 1150623 |
| release and uptake of gene transfer agent by rhodopseudomonas capsulata. | many strains of rhodopseudomonas capsulata are capable of exchanging genetic information via a recently discovered gene transfer process involving the release and subsequent uptake from the medium of particles containing genetic information (gene transfer agents, gtas). no viral activities are observed to be associated with this system. an assay has been developed to quantitate gene transfer in r. capsulata. conditions are described for which the number of cells acquiring a new genetic trait is ... | 1975 | 1150627 |
| formation of reaction centers and light-harvesting bacteriochlorophyll-protein complexes in rhodopseudomonas capsulata. | formation of the photosynthetic apparatus was induced in aerobically grown dark cultures of rhodopseudomonas capsulata by lowering of the oxygen tension. besides the wild type strain the carotenoid-less mutant strain a1a+ was investigated. both strains exhibited initially a decrease of the molar ratio of total bacteriochlorophyll (bchl) to reaction center (rc) bchl, followed by an increase. synthesis of rc-bchl preceded the synthesis of light-harvesting (lh) bchl. activities of photophosphorylat ... | 1975 | 1156096 |
| the metabolism of benzoate by moraxella species through anaerobic nitrate respiration. evidence for a reductive pathway. | moraxella sp. isolated from soil grows anaerobically on benzoate by nitrate respiration; nitrate or nitrite are obligatory electron acceptors, being reduced to molecular n2 during the catabolism of the substrate. this bacterium also grows aerobically on benzoate. 2. aerobically, benzoate is metabolized by ortho cleavage of catechol followed by the beta-oxoadipate pathway. 3. cells of moraxella grown anaerobically on benzoate are devoid of ortho and meta cleavage enzymes; cyclohexanecarboxylate a ... | 1975 | 1156389 |
| low-molecular-weight polysaccharide antigens isolated from rhodopseudomonas gelatinosa. | strain-specific low-molecular-weight polysaccharides of different chemical compositions were obtained from cells of nine different wild-type strains of the phototrophic bacterium rhodopseudomonas gelatinosa. the polysaccharides are free of typical capsule components like hexuronic or aminohexuronic acids but contain (except that of strain 39/2) substantial amounts of phosphorus. a number of unusual o-methyl sugars (2-o-methyl-d-galactose, 2,3-di-o-methyl-d-galactose, 2-o-methyl-l-fucose) as well ... | 1975 | 1158844 |
| photochemical reaction centers from rhodopseudomonas capsulata. | a photochemically active bacteriochlorophyll-protein complex (reaction center) has been isolated from the carotenoidless mutant a1a+ of rhodopseudomonas capsulata by treatment of membranes with lauryl dimethyl amine oxide. three proteins with molecular weights of 20,500, 24,000 and 28,000 (molar ratio 1:1:1) were detected in the reaction center preparations. after mild treatment of intracytoplasmic membranes with na-dodecyl sulfate (0.5%, 30 degrees c, 1 min) succeeded by polyacrylamide gel elec ... | 1975 | 1190953 |
| gene transfer agents, bacteriophages, and bacteriocins of rhodopseudomonas capsulata. | thirty-three wild type strains of rhodopseudomonas capsulata were examined for ability to engage in genetic recombination through mediation by "gene transfer agent" (gta) particles. the genetic exchange assays were based on capacity of strains to produce or receive gta required for restoration of photosynthetic growth competence to a non-photosynthetic "white" mutant or for acquisition of resistance to rifampicin. a majority of the strains could either produce or receive gta, and it was demonst ... | 1975 | 1190956 |
| effect of growth conditions on the activation and inactivation of citrate lyase of rhodopseudomonas gelatinosa. | cells of rhodopseudomonas gelatinosa growing with citrate anaerobically in the light contained citrate lyase only in the acetylated, enzymatically active form of this enzyme. after exhaustion of citrate in the culture medium citrate lyase was deacetylated to yield the inactive sulfhydryl (hs) enzyme. acetylation of hs-citrate lyase required light, anaerobic conditions and the availability of citrate as substrate. the acetylation reaction already in progress stopped immediately when the culture w ... | 1975 | 1194229 |
| genetic transfer of nitrogenase-hydrogenase activity in rhodopseudomonas capsulata. | | 1975 | 1207743 |
| control of composition and activity of the photosynthetic apparatus of rhodopseudomonas capsulata grown in ammonium-limited continuous culture. | rhodopseudomonas capsulata was grown either phototropically in the light or chemotrophically in the dark at oxygen tensions of 5 mm and 3 mm hg in ammonium-limited continuous culture. during growth limitation bacteriochlorophyll content of cells and membranes varied dependent on growth rate drastically in chemotrophic cultures. concomittantly, the ratio of membrane protein to total protein varied in the range of 30-41%. this dependence of membrane differentiation on growth rate was less evident ... | 1975 | 1217939 |
| [characteristics of a new species of budding purple bacteria containing bacteriochlorophyll b]. | a culture of phototrophic purple bacteria containing bacteriochlorophyll b has been isolated. the bacterium forms a sessile bud and, along with producing motile swarmer cells, it produces immobile oval cells surrounded with a slime capsule. it grows on media with simple organic substances in the presence of yeast extract or vitamins, under anaerobic conditions in the light or under microaerophilic conditions in the darkness. besides organic substances, the culture assimilates hydrogen sulphide ... | 1975 | 1226139 |
| primary structure of a high potential iron-sulfur protein from the purple non-sulfur photosynthetic bacterium rhodopseudomonas gelatinosa. | the third amino acid sequence of a high potential iron-sulfur protein, that of the non-sulfur purple photosynthetic bacterium rhodopseudomonas gelatinosa, has been determined. it consists of a single polypeptide chain of 74 amino acid residues, which is slightly smaller than the high potential iron-sulfur proteins from the sulfur purple bacteria chromatium vinosum (85 residues) and thiocapsa pfennigii (81 residues). the sequence of the gelatinosa protein is similar to the c. vinosum and t. pfenn ... | 1976 | 1244346 |
| glycerol utilization by a mutant of rhodopseudomonas capsulata. | the glycerol-catabolizing enzymes of a mutant of rhodopseudomonas capsulata were found to be constitutive and modulated coordinately, although apparently not functional in the presence of malate. no difference in glycerol permeation was found between the mutant and wild type. | 1976 | 1245471 |
| isolation and growth rates of methanol utilizing rhodospirillaceae. | 38 pure culture strains belonging to seven species of the rhodospirillaceae were isolated from 39 methanol enrichment cultures inoculated with water and mud samples of different habitats. none of the strains exhibited doubling times shorter than 10 h in methanol-bicarbonate media. | 1976 | 1259511 |
| map of genes for carotenoid and bacteriochlorophyll biosynthesis in rhodopseudomonas capsulata. | the recently discovered gene transfer system of rhodopseudomonas capsulata was used to construct a genetic map of a region concerned with bacteriochlorophyll and carotenoid production. mutants blocked in the biosynthesis of these compounds were isolated, and each was characterized on the basis of pigments accumulated during growth under low po2. one-point, two-point, three-point, and ratio test crosses were performed between various mutant strains, and the results were amenable to conventional g ... | 1976 | 1262313 |
| characterization of the multiple epr line shapes of iron-semiquinones in photosystem 2. | we have compared the temperature-dependence characteristics of the epr signals of qa and qb iron-semiquinones from both purple bacterial and plant photosystems. the data obtained were analyzed and estimates of the splitting parameters of the non-heme fe2+ spin sublevels obtained. the study confirms the similarities of the g = 1.8 qa iron-semiquinone signal (d/k = 15.6 k, e/k = 3.3 k) formed in formate-treated plant photosystem 2 to the signal found in purple bacteria. however, the g = 1.9 qa iro ... | 1992 | 1312858 |
| electrochemical titration of the cytochrome hemes in the rhodopseudomonas viridis reaction center. cyclic equilibrium titrations yield midpoint potentials without evidence for heme cooperativity. | the redox and spectral characteristics of the 4-heme cytochrome c unit of the photochemical reaction center from rhodopseudomonas viridis were studied by a combination of protein electrochemistry and spectroscopy using an ultra thin-layer spectroelectrochemical cell. quantitative and reversible reduction of the high-potential and the low-potential hemes was performed in cyclic titrations to record the optical difference spectra in the alpha-band region. the titration of the absorbance from the h ... | 1992 | 1312949 |
| [comparison of a stat method of analysis of microorganisms for the presence of site-specific restriction endonuclease activity]. | sensitivity of several express-methods used for detection of bacterial endonucleases was compared. the most sensitive method is that employing triton x-100. | 1992 | 1317567 |
| electron paramagnetic resonance studies of carotenoids. | | 1992 | 1331704 |
| effect of temperature on the kinetics of electron transfer from the tetraheme cytochrome to the primary donor in rhodopseudomonas viridis. | the kinetics of electron transfer from the third highest potential heme (c-552, em = +20 mv) to the primary donor (p-960) have been measured by flash absorption spectroscopy in isolated reaction centers of rhodopseudomonas viridis between 300 k and 7 k. the data are analyzed on the basis of three exponential components with a very fast phase (t1/2 = 120 ns) dominating at high temperature and a very slow one (t1/2 = 1.2 ms) at low temperature. this multiphasic behavior is interpreted in terms of ... | 1992 | 1333411 |
| application of three-dimensional molecular hydrophobicity potential to the analysis of spatial organization of membrane domains in proteins: i. hydrophobic properties of transmembrane segments of na+, k(+)-atpase. | a new computer-aided molecular modeling approach based on the concept of three-dimensional (3d) molecular hydrophobicity potential has been developed to calculate the spatial organization of intramembrane domains in proteins. the method has been tested by calculating the arrangement of membrane-spanning segments in the photoreaction center of rhodopseudomonas viridis and comparing the results obtained with those derived from the x-ray data. we have applied this computational procedure to the ana ... | 1992 | 1334655 |
| application of three-dimensional molecular hydrophobicity potential to the analysis of spatial organization of membrane protein domains. ii. optimization of hydrophobic contacts in transmembrane hairpin structures of na+, k(+)-atpase. | a method of packing of transmembrane hairpin helices in proteins is described. the procedure is based on the optimization of hydrophobic contacts calculated using the three-dimensional (3d) molecular hydrophobicity potential technique. to verify the validity of the computational scheme, we calculated relative orientations of membrane-spanning peptides in pairs l2-l3, m2-m3, and m4-m5 from l- and m-subunits of the photoreaction center of rhodopseudomonas viridis and compared the predicted structu ... | 1992 | 1334656 |
| differentiation between transmembrane helices and peripheral helices by the deconvolution of circular dichroism spectra of membrane proteins. | the interpretation of the circular dichroism (cd) spectra of proteins to date requires additional secondary structural information of the proteins to be analyzed, such as x-ray or nmr data. therefore, these methods are inappropriate for a cd database whose secondary structures are unknown, as in the case of the membrane proteins. the convex constraint analysis algorithm (perczel, a., hollósi, m., tusnády, g., & fasman, g. d., 1991, protein eng. 4, 669-679), on the other hand, operates only on a ... | 1992 | 1338977 |
| oriented immobilization of bacterial photosynthetic membrane. | we have examined a method for oriented immobilization of photosynthetic membrane fragments on a solid surface by specific avidin-biotin interaction. photosynthetic membrane fragments from the purple non-sulphur photosynthetic bacterium rhodopseudomonas viridis, of which the h-subunit of the photosynthetic reaction centre was biotinylated, was immobilized on an avidin-adsorbed plate. orientation of the immobilized membrane on the plastic plate was checked by an antisera binding assay that could r ... | 1990 | 1366521 |
| anoxygenic degradation of aromatic substances by rhodopseudomonas palustris. | three strains of the phototrophic purple nonsulfur bacterium rhodopseudomonas palustris were isolated from different environments and were evaluated for their aromatic degradative potential under phototrophic conditions. all three strains (pfr1, pnr4, and mrl1) utilized benzoate, 4-hydroxybenzoate, 4-aminobenzoate, 4-aminophenol, cinnamate, ferulate, phloroglucinol, and 4-dimethylaminobenzaldehyde in the absence of exogenous co2. 4-aminobenzoate and 4-aminophenol served as a carbon and nitrogen ... | 1992 | 1369192 |
| the light-harvesting core-complex and the b820-subunit from rhodopseudomonas marina. part i. purification and characterisation. | the bchla-containing b880-complex (core-complex) of rhodopseudomonas marina (rhodospirillaceae) was isolated with a new purification method. the isolation of the b880-complex was performed by solubilisation of the photosynthetic membranes with the detergent ldao and subsequent fractionated ammonium-sulfate precipitation with about 50% recovery. the b880-complex retained its original spectral properties as revealed with absorption, fluorescence and circular dichroism spectroscopy. furthermore, we ... | 1992 | 1397300 |
| the light-harvesting core-complex and the b820-subunit from rhodopseudomonas marina. part ii. electron microscopic characterisation. | electron micrographs of photosynthetic membranes of the bchla-containing bacterium rp. marina showed a quasi-crystalline structure. the photoreceptor units are arranged in a hexagonal lattice with a reaction center to reaction center distance of 102 +/- 3 a. purified b880-complex was concentrated up to an od880 of 60 which induced the formation of large protein vesicles. the protein complexes within these vesicles were highly ordered and showed a hexagonal lattice with the same center to center ... | 1992 | 1397301 |