| detection of long-term cellular immunity to coxiella burneti as assayed by lymphocyte transformation. | delayed hypersensitivity to the antigens of coxiella burneti, nine mile strain, was demonstrated in human subjects with various past histories of exposure to the organism by using lymphocyte transformation assays. individuals with histories indicating exposure to c. burneti up to 8 years before the study demonstrated marked lymphocyte transformation in vitro to whole-cell antigens consisting of formalin-killed c. burneti phase i and phase ii. these individuals also demonstrated a marked lymphocy ... | 1975 | 1112616 |
| [q fever laboratory infection (author's transl)]. | | 1975 | 1120327 |
| measurement of renal interstitial fluid pressure with polyethylene matrix capsules. | the renal interstitial fluid pressure was measured in dogs from small chronically implanted solid capsules made of porous polyethylene matrix material (pore size approximately 60 mum). tissue does not grow into the polyethylene and the matrix creates a permanent fluid-filled space in communication with the interstitium. the fluid pressure in the capsule matrix was measured from a tube leading from the capsule with an active servo-null pressure-measuring device. renal interstitial fluid pressure ... | 1975 | 1126909 |
| letter: glomerulonephritis associated with coxiella burnetii tendocarditis. | | 1975 | 1131587 |
| effect of lathyrogens on calcium metabolism in rats. | | 1975 | 1142145 |
| [purification of coxiella burneti antigen by density gradient centrifugation (author's transl)]. | coxiella burneti, strain henzerling, phase ii, was recovered from yolk sacs of infected chidken embryos by mechanical separation, treated with freon and purified by density gradient centrifugation. a j-21 beckman centrifuge was applied and the jcf-z rotor with a zonal as well as a continuous flow core was used. the technique of producing different types of gradients with a wide range of density (1-60% sucrose) without a gradient pump is described. with the zonal core a good separation of antigen ... | 1975 | 1146448 |
| q fever (coxiella burnetii) investigations in dairy cattle: persistence of antibodies after vaccination. | the immune response and persistence of antibodies were investigated in dairy cattle vaccinated with formalin-inactivated phase (ph) i coxiella burnetii vaccine agglutinating antibody geometric mean titer (gmt) of 193.2 at 1 month after vaccination compared to a gmt of 2.0 for nonvaccinated calves. the agglutinating antibodies gradually decreased in vaccinated cattle, but the gmt remained approximately 4 times higher than that for the nonvaccinated group for at least 20 months. results of serotes ... | 1975 | 1147331 |
| pathophysiologic features of q fever-infected guinea pigs. | guinea pigs infected with 9-mile phase i strain of coxiella burnetii had increased blood glucose concentrations; alkaline phosphatase (alp), glutamic-oxalacetic transaminase (got), alpha-hydroxybutyrate dehydrogenase (alpha-hbdh), and creatine phosphokinase (cpk) activities; and bilirubin value. hypocalcemia and hypophosphatemia were evident in the latter days of infection. at necropsy of the guinea pigs, necrotic foci were in liver, spleen, and heart. seemingly, the major pathophysiologic chang ... | 1975 | 1147349 |
| changes in buoyant density relationships of two cell types of coxiella burneti phase i. | coxiella burneti phase i, purified from a formalin-inactivated yolk-sac vaccine, was separated into two bands of morphologically distinct cell types when subjected to sucrose gradient centrifugation. recycling of the less dense, rod-shaped cells in unbuffered sucrose gradients (ph 5.5 to 6.0) resulted in the formation of bands having the location and appearance of the original two bands. recycling of the denser band of larger ovoid-shaped cells yielded a single band, suggesting that the larger c ... | 1975 | 1150325 |
| esterification and etherification by silver oxide-organic halide reaction gas chromatography. | | 1975 | 1150828 |
| [the effect of some antibiotics on multiplication of coxiella burneti in the embryonated egg (author's transl)]. | in chick embryos 7 days after cultivation, the effect of chloramphenicol, streptomycin, tylosin (0.5 to 32 mg/egg), and of penicillin (100 to 10000 ie/egg) on the process of replication of c.b. in the yolk sac was examined. the eld50 and the eid50 were examined according to reed and muench. the demonstration of coxiellae was performed microscopically. the results of the examinations are summarized in fig. 1 to 3. in infected untreated controls, the titres of the eld50 were found at about 10(4) a ... | 1975 | 1154906 |
| [effect of infant foods enrichment with iron salts on hemoglobin and hematocrit values and serum iron levels in young infants]. | | 1975 | 1160233 |
| the pathologic findings in rickettsial pneumonia. | a fatal case of q fever pneumonia with demonstration of the organisms in the lungs is presented. the disease clinically simulates bacterial lobar pneumonia. neutrophils were conspicuously absent in the inflammatory process. histiocytes predominated in the alveolar septal spaces and produced both radiologically and pathologically an alevolar infiltrate. histiocytic hyperplasia and focal necrosis were present. histiocytes similar to those seen in the alveolar spaces of the lungs distorted the norm ... | 1975 | 1163485 |
| comparative morphology of genital cones of genus ancylostoma dubini, 1843. | male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ... | 1975 | 1166351 |
| studies on microperoxisomes. vii. pigment epithelial cells and other cell types in the retina of rodents. | the pigment epithelial cell of the retina actively participates in two aspects of lipid metabolism: (a) the fatty acid esterification of vitamin a and its storage and transport to the photoreceptors, and (b) the phagocytosis and degradation of the lipoprotein membrane disks shed from the photoreceptor cells. study of the pigment epithelial cells of adult albino and pigmented rodents has revealed the abundance of an organelle, microperoxisomes, not previously known to exist in this cell type. the ... | 1975 | 1168648 |
| [observations on the behaviour of coxiella burneti in the argasid tick ornithodoros moubata (author's transl)]. | based on a long-term study of many years, a report is given on the behaviour of coxiella burneti in the argasid tick, ornithodoros moubata. recent observations were made on ticks infected on mice and subsequently maintained separately. particular emphasis was placed upon the localization of the ricksettsiae in certain organs of the ticks, its excretion -also with regard to routes of transmission - and transovarial passage. c. burneti invade primarily the gut epithelial cells of the tick and thes ... | 1975 | 1172309 |
| proceedings: q fever. | | 1975 | 1172513 |
| noxious effects of excessive currents used for intracortical microstimulation. | | 1975 | 1174991 |
| endogenous prostaglandin release contributes directly to coronary artery tone. | an in vitro coronary artery preparation of beef heart was found to synthesize and release continuously large amounts of a prostaglandin of the e type. inhibition of prostaglandin synthesis with aspirin, indomethacin, or eicosa-5,8,11,14-tetraynoic acid induced a sustained contraction of the coronary artery, and pretreatment with indomethacin diminished markedly the output of prostaglandin into the bathing medium. it appears that prostaglandin e1, generated from within the vessel wall itself, may ... | 1975 | 1175084 |
| brain 2-phenylethylamine as a major mediator for the central actions of amphetamine and methylphenidate. | | 1975 | 1196013 |
| toxoplasma gondii and coxiella burneti antibodies among brazilian slaughterhouse employees. | previous studies have indicated toxoplasmosis and q fever are potential hazards to persons who handle raw meat or who work in slaughterhouses. the prevalence of antibodies to t. gondii and c. burneti was studied among 144 employees of an abattoir in belo horizonte, brazil, to determine what environmental factors may be associated with the occurrence of these agents among persons who handle meat but who have not reported having toxoplasmosis or q fever. seventy-two per cent of the 144 employees w ... | 1975 | 1200023 |
| electron microscopic study in guinea-pig cells during infection or vaccination with coxiella burneti. | | 1975 | 1212070 |
| [experimental study of the effectiveness of combined immunization against q fever with living and killed vaccines]. | | 1975 | 1221719 |
| changes of some hepatic and renal mitochondrial enzymes following infection or vaccination with coxiella burneti. | | 1975 | 1227444 |
| vaccination of cattle against q-fever. | formalin-killed phase i c. burneti organisms containing the protective antigenic component were used for vaccination of heifers against q fever. none of the vaccinated heifers contracted q fever, whereas 8 out of 12 (66.6%) control, non-vaccinated heifers became infected when exposed to infection for 3 months in the breed of naturally infected dairy cows. in the vaccinated cattle c. burneti was detected immediately post partum neither in the placenta nor in the colostrum. no shedding of c. burne ... | 1975 | 1236883 |
| [multiplication of coxiella burneti in different tissues and organs of embryonated eggs (author's transl)]. | multiplication and content of coxiella burneti after infection of the yolk sac were studied in different tissues and organs of embryonated hen's eggs (yolk sac, cam, allantoamnion, amniotic fluid, liver, muscles, spleen, and heart). demonstration of the agent was performed from the 6th to the 11th day p.i. by direct microscopic methods (stamp and auramine staining, fluorescent antibody technique); the coxiella content was determined by titration in embryonated hen's eggs. coxiellae could be demo ... | 1975 | 1237206 |
| [morphological ultrastructural and immunological studies of a rickettsia isolated from a parrot (author's transl)]. | a rickettsia isolated from a parrot was found by use of electron microscopic and serological technic to be a coxiella burneti. in cell culture one can see a dramatic polymorphism and a gram negative-like structure of the cell wall. they divide by binary fission with no appearance of a complexe cycle of development like the one described for chlamydia psittaci. many myelinic fibrils and altered coxiella suggest that the host cell react very deaply. | 1975 | 1238044 |
| [studies on the retention of r. burneti in rh. bursa and h. detritum ticks]. | the role played by ixodes ticks in the epizootiology and epidemiology of q fever was studied by following up the transphasal and transovarial passing of rickettsia burnetii in ticks of the rhipicephalus bursa and hyalomma detritum species. use was made of the 269th passage of the rickettsial strain bp, isolated in this country from sheep. the infection was followed up from larva ist generation to larva iind generation of the two ticks. in 10 out of the eleven samples of ticks studied there were ... | 1975 | 1240691 |
| [new findings concerning the spread of natural foci of infection in the transpolar region of eastern siberia]. | new materials are presented on the presence of the foci of anthropozoonozes in the extreme north. for the first time there was established the existence in the subarctic tundra of the taimyr peninsula of the arbovirus foci of the tick-borne encephalitis complex. a virus of the tick-borne encephalitis complex was isolated in 1973 from the gamasida ticks haemogamasus ambulans thorel. and hirstionyssus isabellinus oudms. and the nests of the siberian lemming lemmus lemmus l. this pointed to the exi ... | 1975 | 1243720 |
| [q-fever and its complications (author's transl)]. | q-fever recurs as localized epidemics in the german federal republic. the disease has not previously been observed in north hesse. during a familial infection with coxiella burneti a 15-year-old boy together with his parents and an aunt became ill with atypical pneumonia. after the pulmonary symptoms had subsided a deep left leg and pelvic venous thrombosis occurred in the boy in the fifth week after the onset of the disease. the thrombosis which is very rare in childhood could be successfully r ... | 1976 | 1248390 |
| quantitative correlations between chemical structure and affinity for acetylcholine receptors. | the affinity constants (log k, pa2) of 128 quaternary ammonium compounds belonging to several different series have been correlated linearly with the hydrophobicity (pir) constant, the dipole moment (mur), and the number of hydroxyl groups (noh) of the side chain; the dependence on the hydrophobicity constant of the quaternary ammonium head (pi-n identical to) is shown to be parabolic. a correlation coefficient of 0.96 is obtained for all the compounds using only 4 independent variables (6 terms ... | 1976 | 1248503 |
| q fever endocarditis in queensland. | thirteen patients with proven q fever endocarditis and three additional patients with probable endocarditis are reviewed. the most helpful diagnostic test is the demonstration of a high complement fixing antibody titre to phase 1 antigen of coxiella burneti. the macroscopic pathology of the aortic valve is described and includes aneurysmal pockets in the aortic wall and valve annulus which are demonstrable angiographically. evidence is presented that the infection may be controlled by prolonged ... | 1976 | 1253390 |
| biological properties of the m-44 strain of coxiella burneti. | the attenuated m-44 strain of coxiella burneti was investigated for biological characteristics affecting its use as a live vaccine. after 25 serial passages in guinea pigs, the strain did not develop phase i properties detectable by the microagglutination test. infectious organisms were not detected in untreated guinea pigs later than 12 weeks after inoculation; however, infection could be reactivated at later times by the production of stress (by pregnancy or drug treatment) in guinea pigs that ... | 1976 | 1254991 |
| effects of atropine, injected into a lateral cerebral ventricle of the rabbit, on fevers due to intravenous leucocyte pyrogen and hypothalamic and intraventricular injections of prostaglandin e1. | 1. cholinergic synapses in the hypothalamus may transmit information in those thermoregulatory pathways which function to raise body temperature. the effect of atropine, administered intracranially, on the febrile response to intravenous leucocyte pyrogen or intracranial prostaglandin e1 was therefore examined in conscious rabbits. 2. in rabbits exposed to a thermoneutral environment, micro-injections of pge1, into the anterior hypothalamus, intraventricular injections of pge1, and intravenous i ... | 1976 | 1255503 |
| the detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with dirofilaria immitis antigens. | counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. antigens used in the test were prepared from microfilariae and adult male d. immitis. some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. the presence of soluble circulating ... | 1976 | 1262695 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| an autoclavable intensive care isolator for the adult gnotobiotic dog. | a lightweight stainless steel isolator which allows 3 investigators simultaneous access to an adult gnotobiotic dog for the purpose of rendering intensive care was designed and constructed. this isolator is 54'' high x 60'' long x 42'' wide. it has an 18'' diam entry port at 1 end and a pair of gloves mounted beneath a window on each of the other 3 sides. the chamber rests on a 12'' high pallet and is moved about the laboratory by a portable hydraulic platform lift. it can be rapidly cleansed wi ... | 1976 | 1263433 |
| [methods of studying the electrical activity of the stomach and duodenum]. | | 1976 | 1272972 |
| [cryosurgery of the esophagus and the cardia in animal experiments]. | | 1976 | 1272986 |
| measurement and alteration of the capacity of the distended biliary tree in the rat. | | 1976 | 1273853 |
| regeneration of an adult peripheral nerve preparation in culture. | the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process. | 1992 | 1282333 |
| [the antimicrobial cationic proteins of the neutrophilic granulocytes in experimental q rickettsiosis]. | blood of 56 guinea pigs with experimental q rickettsiosis was studied cytochemically (lysosomal cationic test) to measure the level of cationic proteins in neutrophil granulocytes. development of q rickettsiosis resulted in a decrease in the killing ability of neutrophils, depending on infection dose introduced. however, by day 7 of the disease, the level of cationic proteins in blood neutrophil granulocytes returned to the initial range. similar situation was noted after subcutaneous injection ... | 1992 | 1288699 |
| dog-transmitted zoonoses: a serological survey in the province of bologna. | eight hundred and two sera of owned dogs from four small towns north-west of bologna were examined for the presence of antibodies against coxiella burnetii and leishmania infantum. at the same time, 149 sera of 69 dog owners from the same area were tested for the presence of antibodies to q fever, leishmaniasis, echinococcosis/hydatidosis and toxocarosis. of the 802 dog sera, 7 (0.87%) were positive for q fever. of the 69 dog owners, 24 (35%) were positive for q fever, 4 (6%) for echinococcosis/ ... | 1992 | 1303042 |
| [dependence between penetration route and course of infection with coxiella burnetii in mice]. | this study was aimed at investigation of course of coxiella burnetii infection in mice infected by these bacteria by different routes. the animals infected intranasally, perorally, intraperitoneally and intravaginally by suspension of c. burnetii cells. mice were also infected via peritoneal and intravaginal route with spermatozoa derived from infected males. in all animals at the same time specific antibodies against phase i and phase ii antigens of c. burnetii belonging to igg and igm classes ... | 1992 | 1305918 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| characteristics of neisseria gonorrhoeae isolated in australia showing decreased sensitivity to quinolone antibiotics. | forty three strains of neisseria gonorrhoeae with decreased sensitivity to quinolone antibiotics were detected amongst 2141 australian isolates of gonococci examined in the years 1984 to 1990. the strains examined belonged to 23 different auxotype/serovar classes, were generally more resistant to other antibiotics and, in the majority of cases, were isolated from travellers entering or returning to australia from se asia. quinolone-sensitive wild-type gonococci became less sensitive to these age ... | 1992 | 1315946 |
| new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus. | two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ... | 1992 | 1319172 |
| a coxiella burnetti repeated dna element resembling a bacterial insertion sequence. | a dna fragment located on the 3' side of the coxiella burnetii htpab operon was determined by southern blotting to exist in approximately 19 copies in the nine mile i genome. the dna sequences of this htpab-associated repetitive element and two other independent copies were analyzed to determine the size and nature of the element. the three copies of the element were 1,450, 1,452, and 1,458 bp long, with less than 2% divergence among the three sequences. several features characteristic of bacter ... | 1992 | 1324903 |
| susceptibility of rickettsia conorii, r. rickettsii, and coxiella burnetii to pd 127,391, pd 131,628, pefloxacin, ofloxacin, and ciprofloxacin. | plaque formation and dye uptake assays were used to measure the mics of pd 127,391 and pd 131,628 against rickettsia species. the mics of pd 127,391 were 0.25 microgram/ml for rickettsia rickettsii and 0.125 to 0.25 microgram/ml for rickettsia conorii. the mics of pd 131,628 were 0.25 to 0.5 microgram/ml for r. rickettsii and 0.5 microgram/ml for r. conorii. as determined by the shell vial technique, 15 strains of coxiella burnetii were susceptible to pd 127,391 and pd 131,628 (mic, < or = 1 mic ... | 1992 | 1336950 |
| [biological products and laboratory reagents dehydrated by a rapid direct vacuum desiccation method. vi. studies of the preservation of microbial strains (c. burnetii, chlamydia psittaci, mycoplasma pneumoniae) and a laboratory reagent (para-nitrophenyl phosphate) by vacuum desiccation]. | | 1992 | 1340260 |
| the influence of environmental temperature on the transient effects of methamphetamine on dopamine levels and dopamine release in rat striatum. | when male rats were injected four times (once every 2 hr) with 5 mg/kg methamphetamine (meth) at an environmental temperature of 23 degrees c, transient changes occurred in the levels of striatal dopamine (da) and the regulation of striatal da release. striatal da levels were minimally affected 1 day after meth treatment, but 3 days after meth treatment, striatal da levels decreased to approximately 40% of control. da levels returned to 70% of control 2 weeks after meth. similarly, striatal tyro ... | 1992 | 1346646 |
| dynamics of non-specific antibacterial activity of the peritoneal cells of mice induced with coxiella burnetii antigen. | it was found that primary immunization with coxiella burnetii antigen increased mouse resistance to salmonella typhimurium infection as evidenced by acceleration of bacterial elimination from the peritoneal cavity and a decrease in lethality of experimental animals. the existence of two rises of bactericidal activity of mouse peritoneal cells was ascertained: the "early" on days 1 and 2, and the "late" on day 14 after c. burnetii administration. the first rise was accompanied by some increase in ... | 1992 | 1350168 |
| ultrastructure of viable and inactivated coxiella burnetii after inoculation of mice. | c3ha mice were inoculated intraperitoneally (i.p.) with viable coxiella burnetii (c.b.) (strain apodemus flavicollis-luga, phase i) or c.b. antigen (formalin-inactivated c.b.); pieces of spleen, liver, omentum, kidney, and suspension of peritoneal macrophages were examined by electron microscopy at 1, 7, 14, 21, 28, and 42 days post-infection (p.i.). two main types of viable c.b. cells--small dense (dc) and bacteria-like (bc)--were found in omentum, spleen, and peritoneal exudate cells beginning ... | 1992 | 1350169 |
| immunoblot analysis of antibody response in mice infected with coxiella burnetii phase i. | mouse sera collected from day 4 to day 133 postinfection (p.i.) with phase i coxiella burnetii strain nine mile were analysed by immunoblotting with phase i c. burnetii cell lysate. antibodies of igg class protein antigens were revealed already on day 10 p.i. (60, 49 and 27 kd proteins), followed by those to 77 kd protein from day 18 p.i. and to further 7 (from 42 to 70 kd) proteins from day 28 p.i. igg antibody reaction was observed also with 5 antigens in 14-20 kd region corresponding to lipop ... | 1992 | 1350170 |
| phenotypic and genotypic heterogeneity of 8 new human coxiella burnetti isolates. | eight new strains of coxiella burnetii were isolated from chronic q fever patients using centrifugationashell vial technique. seven patients had endocarditis (including one patient with an immunodeficiency syndrome), and one had a vascular prosthesis infection. three prototype strains, nine mile phase ii, q212 and priscilla and eight new isolates were cultured in l 929 cells. heterogeneity of their cytopathic effect was observed. dnas of the eleven strains have been isolated and purified by stan ... | 1992 | 1350176 |
| immunoblot analysis of igg subclass antibody response against coxiella burnetii in balb/c mice. | balb/c mice were inoculated with live or inactivated organisms of coxiella burnetii, strain nine mile, phase i. sera collected after different time intervals were subjected to immunoblot analysis and results compared with elisa values. immunoblots were performed with different horseradish peroxidase labelled conjugates (goat anti-mouse igg1, igg2a, igg2b, igg3) and results monitored and analysed by laser densitometry. elisa analysis was performed using the same peroxidase labelled goat anti-mous ... | 1992 | 1350177 |
| influence of coxiella burnetii infection of male mice on their offspring. | the dramatic spread of q fever in poland among cattle kept in isolation from natural environment (ticks, wild animals) has suggested the possibility that the infection may also be transmitted sexually. to test this hypothesis series of experiments have been performed in controlled laboratory conditions. male mice infected with c. burnetii were allowed to mate with healthy female mice. on day 18 of pregnancy serum igm antibodies to c. burnetii antigens and bacteria in spleen, liver and placenta w ... | 1992 | 1350178 |
| dna probes for detecting coxiella burnetii strains. | methods have been developed for the rapid detection of c. burnetii by specific hybridization of labelled dna probes to rickettsial plasmid dna sequences present in clinical samples. one dna probe detects all c. burnetii strains, while additional probes differentiate, between organisms associated with chronic or acute disease. using these probes, c. burnetii can be identified in blood, urine, and tissue samples. the plasmid-derived dna probes detect as few as 10(4) organisms and less than 1 ng of ... | 1992 | 1350179 |
| molecular characterization of cloned variants of coxiella burnetii isolated in china. | to study the molecular properties of coxiella burnetii phase variants we cloned the phase variants of c. burnetii qiyi (cbqy) strain by the red plaque technique. three cloned strains, cbqyic3 (phase i), cbqyiic7 (phase ii) and cbqyiic5 (semirough-phase) were analysed by sds-page, immunoblot assay, plasmid isolation and agarose gel electrophoresis of dna restriction fragments. the results suggest that the unique phase-dependent substance is a lipopolysaccharide and that most protein components of ... | 1992 | 1359769 |
| basic structural features of a lipopolysaccharide from the coxiella burnetii strain nine mile in the virulent phase i. | | 1992 | 1359776 |
| a randomized trial of reduced doses of azidothymidine in japanese patients with human immunodeficiency virus type 1 infection. | adverse reactions to the standard dose (1,200 mg/day-1,500 mg/day) of azidothymidine (azt) are serious. an in vitro pharmacokinetic study of intracellular azt-5'-triphosphate suggested the feasibility of a clinical trial with reduced doses of azt. a randomized trial with reduced doses of azt (group a; 400 mg/day, n = 15, group b; 800 mg/day, n = 13), was conducted enrolling 28 patients with human immunodeficiency virus infection. the effective rate of azt on cd4+ lymphocyte counts was similar fo ... | 1992 | 1360277 |
| study of cross-reaction between coxiella burnetii and legionella pneumophila using indirect immunofluorescence assay and immunoblotting. | patients with q fever and legionellosis may present identical clinical symptom. differentiation of these diseases is made by serology, mainly the indirect immunofluorescence assay (ifa). using ifa the authors tested 154 q fever positive sera from 55 patients with acute q fever and 28 patients with chronic q fever for legionella pneumophila antibodies and 57 sera from 57 patients with legionellosis for coxiella burnetii antibodies. of the 211 sera tested, four sera from different patients had ant ... | 1992 | 1364022 |
| attempt to infect hunterellus hookeri howard (hymenoptera, encyrtidae), an endoparasite of ticks, with coxiella burnetti. | | 1992 | 1364029 |
| piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes. | a novel intracellular pathogen morphologically similar to the ehrlichiae has been isolated in cell culture and identified as the cause of an epizootic disease of salmonid fish. like the ehrlichiae, the salmonid pathogen, designated strain lf-89, replicates within membrane-bound cytoplasmic vacuoles in host cells. this agent is the first with characteristics of this type to be isolated from a fish. analysis of the lf-89 16s rrna indicated that, unlike the ehrlichiae, lf-89 is a gamma proteobacter ... | 1992 | 1371057 |
| [seroprevalence of q fever among the adult population of lanzarote (canary islands)]. | q fever is an endemic zoonosis in the canary islands. in 1986, we detected, in a pilot study, residual antibodies of the infection in 3% of the population from lanzarote. in 1989, we performed a new study in order to assess seroprevalence of q fever among the adult native population from the island. we studied 390 human serums obtained from an statistically representative sample. age ranged from 30 to 64 years. out of 390 serums, 196 (50.25%) were obtained from men and 194 (49.74%) from women. t ... | 1992 | 1391577 |
| [prolonged febrile syndrome as a manifestation of coxiella burnetii infection, presentation of infrequent clinical cases]. | we present two cases of infection by coxiella burnetii which developed with sustained fever symptoms. during its evolution, the first case presented granulomatous hepatitis, whereas the second case presented left cosofemoral arthritis. we describe the clinical-evolutive characteristics of these clinical forms and within the evolution of the chronic forms of q fever. | 1992 | 1391579 |
| monoclonal antibody based capture elisa/elifa for detection of coxiella burnetii in clinical specimens. | a capture elisa/elifa system based on monoclonal capture and biotinylated monoclonal detection antibody is described. the assay is fast, highly specific and detects a minimum dose of 2500 coxiella (c.) burnetii particles. in contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories could use this assay system for investigating clinical samples of different origin for c. burnetii within a short period of time. | 1992 | 1397227 |
| enzyme immunoassay for q fever: comparison with complement fixation and immunofluorescence tests and dot immunoblotting. | enzyme-linked immunosorbent assays (elisa) for the detection of specific immunoglobulin g (igg) and igm antibodies were developed by using purified coxiella burnetii cells. variables, including type of microtiter plate, blocking agent, incubation conditions, antigen stability, and substrate type, were examined to achieve optimal elisa performance. the reliabilities of the assay systems were compared with those of complement fixation (cf) and enhanced immunofluorescence (eif) tests with 600 human ... | 1992 | 1401014 |
| detection of coxiella burnetti by dna amplification using polymerase chain reaction. | the polymerase chain reaction (pcr) was used for the detection of coxiella burnetti, an obligate intracellular bacterium and the etiologic agent of q fever. a pair of primers derived from the c. burnetii superoxide dismutase gene served to amplify a targeted 257-bp fragment of genomic dna. these primers were chosen on the basis of genbank analysis, g + c ratio, and absence of secondary structure. this technique allowed the detection of as few as 10 c. burnetii organisms. c. burnetti was detected ... | 1992 | 1401016 |
| phagolysosomal alkalinization and the bactericidal effect of antibiotics: the coxiella burnetii paradigm. | most infections due to intracellular bacteria respond poorly to antibiotic treatment. the chemical conditions within the subcellular site of bacteria may change antibiotic activity. coxiella burnetii multiplies within phagolysosomes. the antimicrobial activity of antibiotics combined with the lysosomotropic agents amantadine (1 microgram/ml), chloroquine (1 microgram/ml), and ammonium chloride (1 mg/ml), which alkalinized coxiella burnetii-containing phagolysosomes from ph 4.8 to 5.3, 5.7, and 6 ... | 1992 | 1402021 |
| a simple method for amplification of dna from paraffin-embedded tissues. | | 1992 | 1408846 |
| epidemiologic features and clinical presentation of acute q fever in hospitalized patients: 323 french cases. | to contribute to the knowledge of epidemiologic and clinical features of patients hospitalized with q fever in france. | 1992 | 1415306 |
| susceptibility of some species of rodents to rickettsiae. | the present study was designed to test the susceptibility of free-living rodents, viz apodemus flavicollis, microtus arvalis, clethrionomys glareolus, mus musculus, and outbred white mice from dobrá voda farm, csfr, to coxiella burnetii, rickettsiae of the spotted fever group (rickettsia sibirica, r. conorii, r. slovaca and r. akari) and rickettsiae of typhus group (r. typhi and r. prowazekii) by various routes of administration. the highest levels of antibodies to c. burnetii were found in a. f ... | 1992 | 1427494 |
| rapidly fatal q-fever pneumonia in a patient with chronic granulomatous disease. | acute q-fever is a systemic illness which rarely has a fatal outcome. fatal cases do occur with the chronic form of the disease and associated with endocarditis. this report presents the case of a fatal, acute q-fever pneumonia in an 11-year-old patient with chronic granulomatous disease. complement fixation antibody titer rose to 1:1,024 with positive igm in immunofluorescence. giemsa stained lung sections and indirect immunofluorescence demonstrated the microorganisms in the tissues. the coxie ... | 1992 | 1428185 |
| structure and function of the cd16:zeta:gamma complex expressed on human natural-killer cells. | natural-killer (nk) cells are large granular lymphocytes involved in host defense against tumor cells and virally-infected cells. in addition to natural cytotoxicity, nk cells can effect antibody-dependent cytotoxicity mediated by cd16 (fc gamma riiia alpha). it has recently been shown that cd16 is associated with disulfide-linked dimers composed of 2 homologous sub-units, zeta and gamma. these transducing molecules are also associated with other multimeric cell-surface receptors such as the t-c ... | 1992 | 1428397 |
| [is a29, b12 vasculitis caused by the q fever agent? (coxiella burnetii)]. | the authors describe the first two cases, to their knowledge, of retinal vasculitis, associated with q fever. the first case was a chronic infection induced by coxiella burnetii associated with hla group a29 and b12; in the second case, the phenotype was b12. the authors tried to determine whether this agent could be the "birdshot chorioretinopathy" promoting factor. several epidemiologic findings tend to prove that q fever is not the only cause. on the other hand, its similarity with rickettsia ... | 1992 | 1430809 |
| late airway changes caused by chronic rejection in rat lung allografts. | airway disease after lung or heart-lung transplantation is one of late major complications, affecting the prognosis of the transplants. little is known about the causes of airway changes. we performed rat lung transplantation and investigated the late airway changes of the long-term surviving lung grafts: allografts, bn to lewis; isografts, bn to bn rat. all recipients were treated with csa. we found airway changes, i.e., mucosal ulceration, granulation, submucosal fibrosis, which was located in ... | 1992 | 1440846 |
| spontaneous splenic rupture complicating acute q fever. | q fever is usually a self-limited febrile illness that involves the lungs and the liver. acute complications are rare. we present the case of a 30-yr-old patient with spontaneous splenic rupture during the course of acute q fever infection. he was admitted to the hospital with high temperature and the radiological signs of an atypical pneumonia. forty-eight hours after admission, he developed shock. because of free intraabdominal liquid, a laparatomy was performed that revealed a tear in the enl ... | 1992 | 1442694 |
| q fever endocarditis: diagnostic approaches and monitoring of therapeutic effects. | the scope of current diagnostic methods for q fever endocarditis includes serology, direct demonstration of coxiella burnetii in the resected heart valve tissue, and animal inoculation studies. illustrated by a clinical case report, the different methods are presented and discussed. serology represents the primary method, using the techniques of complement fixation, indirect immunofluorescence, and enzyme-linked immunosorbent assay (elisa). the latter two techniques allow the detection of immuno ... | 1992 | 1450617 |
| phagolysosomes of coxiella burnetii-infected cell lines maintain an acidic ph during persistent infection. | coxiella burnetii, the agent of q fever, is an obligate intracellular bacterium that multiples within vacuoles of phagolysosomal origin. persistently infected cell lines were maintained in continuous culture for months. we studied the ph of the phagolysosomes by using two murine cell lines during early propagation of the bacteria and after establishment of persistent infection. three strains of c. burnetii were studied because of the purported propensity of each strain to cause acute or chronic ... | 1992 | 1452331 |
| histological and immunocytochemical characterization of coxiella burnetii-associated lesions in the murine uterus and placenta. | the fetoplacental units and the postgravid uterus of balb/cj (h-2d) mice inoculated intraperitoneally with coxiella burnetii (nine mile isolate, phase i) on day 6 of pregnancy were examined histologically and immunocytochemically at 1 to 160 days postinoculation. clinically, abortions, stillbirths, and perinatal deaths were observed. histological lesions in the placenta were characterized by severe necrosis of the decidua basalis and the labyrinth, fibrinoid degeneration of decidual vessels, and ... | 1992 | 1452356 |
| laboratory outbreak of q fever. | an outbreak of q fever in a university department where sheep placentas were being used for research is described. of six persons exposed to the sheep, four had positive titers with only one person developing an acute febrile illness and liver disease. this report illustrates the value of the family physician obtaining an occupational history and conducting an outbreak investigation. | 1992 | 1453153 |
| [the role of tumor necrosis factor and interleukin-1 in forming resistance in experimental q fever]. | preliminary injections of human recombinant tumor necrosis factor (tnf-alpha) and interleukin 1 (il-1 beta) increases the resistance of guinea pigs to coxiella burnetii. the increase of this resistance depends on the dose of cytokines and the time of their inoculation. | 1992 | 1455970 |
| interlaboratory study of the ifcc method for alanine aminotransferase performed with use of a partly purified reference material. | we present the results of a study on performance of a reference material for alanine aminotransferase (alt, ec 2.6.1.2) and the corresponding ifcc-approved method in an interlaboratory trial involving 13 laboratories. the alt material was partly purified from pig heart (specific activity, 150 ku/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (ec 2.6.1.1). the partly purified alt was lyophilized in a triethanolamine-buffered ma ... | 1992 | 1458569 |
| unusual presentation of tuberculous peritonitis. | | 1992 | 1459703 |
| [seroepidemiological studies on the detection of q fever in sheep in middle thuringia]. | in a random samples test altogether 4337 sheep of varying ages from several herds of different sizes in the middle region of thuringia were investigated with complement fixing test for the existence of q fever. in 47.1 per cent of the tested herds and in 5 from 8 of the included districts q fever reagents were provable. the serological detection quota of all tested sheep amounts to 1.11 per cent, but the percentage of serological reagents in mother-sheep was 1.36 per cent, in the female young sh ... | 1992 | 1463436 |
| reactivation of q fever following cardiac surgery. | to determine whether reactivation of q fever occurs following cardiac surgery 43 patients who had antibodies to coxiella burnetii were identified pre-operatively. serum samples were collected 1, 4 to 6, and 24 weeks post-operatively. one patient had active q fever endocarditis. seven of the remaining 42 (17%) had a > or = fourfold rise in titres for antibodies to coxiella burnetii post-operatively. only two of these seven had an increase in antibody titres for other agents suggesting that the fo ... | 1992 | 1468423 |
| seroepidemiology of coxiella burnetii in domestic and companion animals in japan. | | 1992 | 1471330 |
| fibrin ring granulomas in hepatitis a. | fibrin ring granuloma is characterized by a fibrinous ring surrounding a central fat vacuole. it has been found in the liver and bone marrow of patients with q fever, and occasionally with visceral leishmaniasis, cytomegalovirus, epstein-barr virus, staphylococcus epidermidis infections, hodgkin's lymphoma, and hypersensitivity to allopurinol. we describe a case of serologically confirmed viral hepatitis a with this lesion in the liver biopsy. a false positive anti-hepatitis a virus igm result h ... | 1992 | 1473440 |
| increased concentration of plasma cholesteryl ester transfer protein in nephrotic syndrome: role in dyslipidemia. | hyperlipidemia is a prominent feature of the nephrotic syndrome. lipoprotein abnormalities include increased very low and low density lipoprotein (vldl and ldl) cholesterol and variable reductions in high density lipoprotein (hdl) cholesterol. we hypothesized that plasma cholesteryl ester transfer protein (cetp), which influences the distribution of cholesteryl esters among the lipoproteins, might contribute to lipoprotein abnormalities in nephrotic syndrome. plasma cetp, apolipoprotein and lipo ... | 1992 | 1479291 |
| twinning and the r/k reproductive strategy: a critique of rushton's theory. | the theory of r selection, favoring population growth, as opposed to k selection, favoring more efficient utilization of resources, has in recent years been applied by rushton to contrast human ethnic groups in terms of their r/k reproductive strategies, suggesting the existence of a continuum from r groups, producing many offspring but providing little parental care, to k groups, producing few offspring but providing much parental care. rushton's theory, which is largely based on ethnic differe ... | 1992 | 1488860 |
| characterization of the specificity of the human antibody response to the v3 neutralization domain of hiv-1. | the major neutralization domain of hiv-1, contained in the third variable region (v3) of the external envelope, is highly variable at positions flanking a conserved glycine-proline-glycine sequence. we investigated the relation between v3 sequences of hiv-1 variants circulating in a host and that host's antibody specificity. multiple v3 sequences were obtained directly, via pcr and subsequent cloning, from serum rna or cellular dna from 26 individuals (from 12 around seroconversion). then, speci ... | 1992 | 1489578 |
| [lower respiratory infection caused by coxiella burnetti in children]. | | 1992 | 1489786 |
| [granulomatous hepatitis and pericarditis caused by q fever]. | | 1992 | 1489807 |
| improved procedure for the drying and storage of polyacrylamide slab gels. | an improved procedure for the drying and storage of polyacrylamide (paa) slab gels of various sizes and acrylamide contents is presented. a paa slab gel is preferentially fixed in methanol-acetic acid-glycerol-water (1.0:1.0:0.1:2.0) and then dried under low pressure in an adapted photoprint dryer between cellophane and polyethylene foils at 75-80 degrees c for 50-90 min. strong, transparent and flexible sheets are obtained that can be stored for extended periods. the potential of the procedure ... | 1992 | 1491050 |
| coxiella burnetii superoxide dismutase gene: cloning, sequencing, and expression in escherichia coli. | a superoxide dismutase (sod) gene from the obligate intracellular bacterium coxiella burnetii has been cloned, and its dna sequence has been determined and expressed in escherichia coli. the gene was identified on psjr50, a phc79-derived genomic clone, by using the polymerase chain reaction with degenerate oligonucleotide primers corresponding to conserved regions of known sods. sequences resembling conventional e. coli ribosomal and rna polymerase-binding sites preceded the c. burnetii 579-bp s ... | 1992 | 1500190 |
| primary humoral antibody response to coxiella burnetii, the causative agent of q fever. | of 147 patients with acute q fever diagnosed during a major outbreak in birmingham, england, in early summer 1989, 41 provided sets of sera which allowed us to make a detailed analysis of the primary humoral immune response. antibody titers specific for coxiella burnetii were measured by the complement fixation test and by an immunoglobulin m (igm)- and igg-specific indirect immunofluorescence test. the relative avidity of specific iggs was determined by the indirect immunofluorescence test with ... | 1992 | 1500499 |
| electron-microscopic study of the effect of various extractants on the morphology of coxiella burnetii. | this paper presents the results of the effect of trichloroacetic acid (tca) at two different temperatures and the effect of a mixture of detergents (d) on coxiella burnetii. both tca and d caused a large destruction of c. burnetii cells. in both cases complexes of high-molar-mass components from the outer membrane were extracted. in the d case not only proteins but a mixture with other high-molar-mass structures were released from the destroyed cells. by extraction of tca, the antigenic complex ... | 1992 | 1505876 |
| coxiella burneti endocarditis in a patient with positive chlamydial serology. | a 41-year-old man who habitually slept in a car park presented with a culture-negative endocarditis. serological tests indicated infection with both coxiella burneti and chlamydia psittaci. he was treated with doxycycline and clindamycin and required aortic valve replacement. culture of the excised value for both agents was negative but electron microscopy was suggestive of coxiella endocarditis. | 1992 | 1522334 |
| [q fever antibody titer--follow-up study in cattle with special reference to pregnancy]. | in 290 q fever positive cattle from three 2000 head dairy farms in the former district of erfurt (thüringen) the course of titers was examined serologically over several months by means of the complement fixation test (cft). in 47.2% of the cows serologically observed for 2 up to 28 months complement fixing antibodies against coxiella burnetii could be demonstrated til the end of the investigation period. repeated tests during pregnancy showed increase of antibody titers in the first 4 months an ... | 1992 | 1524578 |