| in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i. | the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ... | 1990 | 2203313 |
| massive rhabdomyolysis: a rare presentation of primary vibrio vulnificus septicemia. | | 1990 | 2220890 |
| abelson murine leukemia virus transforms preneoplastic emu-myc transgene-carrying cells of the b-lymphocyte lineage into plasmablastic tumors. | e mu-myc transgenic mice were back-crossed to balb/c mice up to back-cross generation 3. the offspring that included transgene-carrying and -negative mice in approximately equal proportions were randomly divided into 2 groups. thirty-four mice (group i) were treated with pristane, followed by a-mulv, and 40 (group ii) were injected with a-mulv alone. altogether, 16 lymphoid tumors developed in group i and 17 in group ii. nine of the tumors in group i and 4 in group ii appeared as ascitic tumors. ... | 1990 | 2228313 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| proteolytic processing of porcine big endothelin-1 catalyzed by cathepsin d. | cathepsin d, a candidate for endothelin-converting enzyme (ece), was allowed to act on porcine big endothelin-1 (big et-1, 1-39). the proteinase primarily cleaved the asp18-ile19 and trp21-val22 bonds of big et-1(1-39), with a optimum ph of 3.5. the mature et-1(1-21), generated by the cleavage between trp21 and val22, was subsequently degraded by removal of most of the c-terminal tripeptide (ile19-ile20-trp21). therefore, cathepsin d is by no means a specific ece, although the proteinase does cl ... | 1990 | 2268325 |
| a double-blind comparison of 0.5% bupivacaine and 0.75% ropivacaine administered epidurally in humans. | in an attempt to compare equipotent doses of epidurally administered bupivacaine and ropivacaine, 44 healthy patients, aged 18-70 years, undergoing lower extremity orthopedic procedures were studied in a randomized, double-blind manner. twenty-one patients received a single epidural injection of 20 ml 0.5% bupivacaine at the l23 or l34 interspace and 23 patients received 20 ml 0.75% ropivacaine in a similar manner. onset of and recovery from sensory anesthesia and motor block were recorded. no s ... | 1990 | 2271468 |
| a successfully treated case of vibrio vulnificus septicemia with shock. | vibrio vulnificus infection often causes serious or fatal disease. recently, in japan there have been numerous reports of vibrio vulnificus infection. here, we report a successfully treated case of vibrio vulnificus septicemia with shock, disseminated intravascular coagulation (dic) and necrotizing cellulitis in a middle-aged heavy drinker with chronic alcoholic liver disease. on reviewing 38 cases in japan including ours, the overall mortality rate was 68%. although the incidence is relatively ... | 1990 | 2273613 |
| incidence of vibrio vulnificus in northern new england water and shellfish. | vibrio vulnificus, an autochthonous inhabitant of the estuarine environment, was detected in water and oysters from the great bay estuary system of new hampshire and maine. previously, it had not been detected north of boston harbor on the east coast of the united states. v. vulnificus was detected in water and shellfish samples at five out of ten sites, and only in areas that were not open to recreational shellfishing. although samples were collected from may into december, v. vulnificus was on ... | 1990 | 2283033 |
| [bronchial hyperresponsiveness to histamine in guinea pig induced by intravenous administration of platelet activating factor]. | platelet activating factor (paf) has recently been demonstrated to play an important role in allergic diseases such as bronchial asthma. changes in airway wall thickness have recently been suggested to cause excess airway narrowing of asthma. in this study, the authors examined the bronchial hyperresponsiveness to histamine in guinea pig induced by intravenous administration of paf by measuring dynamic compliance and dynamic respiratory resistance. moreover, a new formula which can exclude the e ... | 1990 | 2290229 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| vibrio vulnificus wound infection treated successfully with oral ciprofloxacin. | | 1990 | 2299188 |
| osteomyelitis caused by vibrio vulnificus. | | 1990 | 2299219 |
| polyvibrio infections: vibrio vulnificus and vibrio parahaemolyticus dual wound and multiple site infections. | | 1990 | 2299220 |
| vibrio vulnificus infections in the hand. report of three patients. | | 1990 | 2303515 |
| digestibility of cotton plant by-product diets for sheep at two levels of intake. | crossbred wethers (n = 48) were assigned randomly to 1 of 12 experimental diets, arranged in a 3 x 2 x 2 split plot to evaluate three levels of cotton plant by-product (25, 50 and 75% cpb), two levels of naoh treatment of cpb (0 and 4%) and two levels of monensin supplementation (0 and 30 ppm, dm basis) at two intake levels (70 and 90% of ad libitum). intake was a within-lamb variable. ad libitum intake was established over an initial 17-d adjustment period. feed intake and fecal output were rec ... | 1990 | 2312420 |
| primary vibrio vulnificus sepsis in kentucky. | vibrio vulnificus is associated with infection acquired during contact with sea water or with seafood, and is seldom suspected by physicians in noncoastal states. the ease of transportation of fresh raw seafood has facilitated this organism's capacity to produce disease in geographic areas in which it was previously unseen. we have reported a case of fatal vibrio vulnificus sepsis acquired from ingestion of fresh oysters in the inland united states. | 1990 | 2315791 |
| long-term clearance of inhaled uo2 particles from the pulmonary region of the rat. | this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ... | 1990 | 2323929 |
| casein and casein subunits in preterm milk, colostrum, and mature human milk. | human milk proteins have both nutritional and physiological roles for the breast-fed infant. while the biochemistry and developmental patterns for many whey proteins are well known, our knowledge of human casein and its subunits is still limited. we have recently developed a method to isolate casein from whey proteins in human milk and to separate the casein subunits by fast protein liquid chromatography. in this study we have applied this methodology to study the casein subunit pattern in prete ... | 1990 | 2358977 |
| blockade of inhibition in a pathway with dual excitatory and inhibitory action unmasks a capability for ltp that is otherwise not expressed. | long-term potentiation (ltp) can be readily elicited in a number of hippocampal pathways, but has not been seen in the dentate commissural pathway. the dentate commissural pathway is similar to the commissural/schaffer collateral projection to ca1 except that it produces powerful inhibition that occurs nearly concurrently with the excitation. the present study evaluates whether this inhibition prevents the pathway from expressing ltp. acute neurophysiological experiments were carried out in uret ... | 1990 | 2364294 |
| the cytolysin gene of vibrio vulnificus: sequence and relationship to the vibrio cholerae e1 tor hemolysin gene. | a cytolysin of ca. 56 kilodaltons has been suggested as a possible virulence factor in vibrio vulnificus infections. we sequenced the dna encoding cytolytic activity and found that the sequence contained two open reading frames, vvha and vvhb. vvha encoded the structural gene for the cytolysin and contained the n-terminal amino acid sequence previously reported for the protein. regions of the vvha gene showed homology to the structural gene for the vibrio cholerae e1 tor hemolysin. | 1990 | 2370116 |
| vibrio bacteremia in siriraj hospital. | from january 1983 to march 1988, 26 isolates of vibrio spp. were recovered from the blood of patients admitted to siriraj hospital. thirteen strains were identified as non 0-1 vibrio cholerae, 3 were vibrio vulnificus and 10 were vibrio spp. the medical records of 20 patients were available for clinical analysis. most of them were adult men with cirrhosis. clinical features included fever, abdominal pain, diarrhea, peritonitis, shock and skin lesions. some patients had a history of seafood consu ... | 1990 | 2380645 |
| white noise analysis of temporal properties in simple receptive fields of cat cortex. | we studied the linear and nonlinear temporal response properties of simple cells in cat visual cortex by presenting at single positions in the receptive field an optimally oriented bar stimulus whose luminance was modulated in a random, binary fashion. by crosscorrelating a cell's response with the input it was possible to obtain the zeroth-, first-, and second-order wiener kernels at each rf location. simple cells showed pronounced nonlinear temporal properties as revealed by the presence of pr ... | 1990 | 2390534 |
| increase in carcinoembryonic antigen release from cancer cells by combined treatment with retinoic acid and low-temperature hyperthermia. | the growth of a human gastric adenocarcinoma cell line, mkn-45, was inhibited and the amount of carcinoembryonic antigen (cea) in both the culture medium and the cell extract was increased in the presence of retinoic acid at a concentration of 75 (1.5x)-125 microm (1.9x), which did not substantially affect cell survival. treatment using a combination of retinoic acid (125 microm) and low-temperature hyperthermia (40 degrees c, 30 min) was more effective in increasing cea compared with retinoic a ... | 1990 | 2394926 |
| are separable aromatase systems involved in hormonal regulation of the male brain? | in vitro study of testosterone (t) metabolism shows that formation of estradiol-17 beta (e2) is regionally specific within the preoptic area (poa) of the male ring dove. the poa is known to be involved in the formation of e2 required for specific components of male sexual behavior. two sub-areas of high aromatase activity, anterior (apoa) and posterior preoptic (ppoa) areas, have been identified. aromatase activity is higher in apoa than in ppoa. the aromatase activity within the apoa is also mo ... | 1990 | 2394989 |
| metabolism of indium-111-labeled murine monoclonal antibody in tumor and normal tissue of the athymic mouse. | we have studied the fate of radiometal metabolism in vivo by analyzing the molecular form of radiolabeled anti-carcinoembryonic antigen (cea) in tissues. athymic mice bearing colonic tumor xenografts were injected i.v. with 111in-isothiocyanate-benzyl-dtpa-(scn-bz-dtpa) igg or 111in-(scn-bz-dtpa) f(ab')2, and then killed daily for up to four days. liver, kidney, and tumor were extracted and the supernatants, plasma and urine samples were analyzed by hplc, itlc, hpiec and sds-page. by hplc, the a ... | 1990 | 2395019 |
| catalase enhances damage to dna by bleomycin-iron(ii): the role of hydroxyl radicals. | bleomycin degrades dna under aerobic conditions when a ferrous salt is added. this reaction is enhanced by catalase and certain hydroxyl radical scavengers but inhibited by the addition of hydrogen peroxide. a ferricbleomycin complex is, however, stimulated by addition of hydrogen peroxide. these findings suggest that catalase removes hydrogen peroxide and in so doing prevents loss of ferrous ions and formation of hydroxyl radicals (oh.) by a fenton-type reaction. it further suggests that oh. ra ... | 1985 | 2409975 |
| a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin. | in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative. | 1985 | 2412878 |
| serotonin and 5-hydroxyindoleacetic acid in brains of suicide victims. comparison in chronic schizophrenic patients with suicide as cause of death. | serotonin (5-ht) and 5-hydroxyindoleacetic acid concentrations were determined in various brain areas of nonschizophrenic suicide victims, chronic schizophrenic patients with or without suicide as the cause of death, and normal control subjects without psychiatric or neurologic disorders. serotonin concentrations in the basal ganglia were significantly elevated in suicide victims and chronic schizophrenic patients, as were 5-hydroxyindoleacetic acid concentrations in the occipital cortex. these ... | 1986 | 2423050 |
| identification and characterization of the nmyc gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. | increased n-myc (now designated nmyc in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. as yet, the n-myc gene product has not been identified. to detect and characterize the n-myc gene product, we have developed monoclonal antibodies against the putative n-myc gene product made in escherichia coli as a fusion protein. the antibodies that recognize the n-myc-specific regions were selected on the basis of their reactivities to ... | 1986 | 2426708 |
| [effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties]. | thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ... | 1986 | 2430684 |
| correlation between virulence and colony morphology in vibrio vulnificus. | of 38 isolates of vibrio vulnificus examined, all avirulent strains produced only translucent colonies. all virulent strains, with the exception of biogroup 2 (eel pathogens), exhibited both opaque and translucent colonies. isogenic morphotypes were examined for a variety of phenotypic and virulence traits. only the ability to utilize transferrin-bound iron and the presence of a surface polysaccharide were found to correlate with colony opacity and virulence. | 1987 | 2432016 |
| the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr. | a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo. | 1987 | 2435577 |
| evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the oam. | an antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ... | 1987 | 2446533 |
| influence of neuropeptides on airway smooth muscle. | the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ... | 1987 | 2446543 |
| new differentiation antigens associated with the growth and maturation of b lymphocytes. | monoclonal antibodies (mab) nda3 and nda4 were generated by hyperimmunizing mice with an alloreactive human t cell clone and fusing the splenocytes with the ns1 myeloma. immunofluorescence studies indicated that these mab react with activated, but not with resting t and b lymphocytes or with other types of cells. immunoprecipitation studies with 125-i-labeled extracts from t and b cell lines demonstrated that the m.w. of the antigen recognized by mab nda3 is 36,000 and that of nda4 is 46,000. st ... | 1988 | 2447175 |
| inhibitory effect of alpha 2-macroglobulin on vibrio vulnificus protease. | vibrio vulnificus, an etiologic agent of wound infections and septicemia in humans, elaborates a metalloprotease which is known to be an important virulence factor of the vibrio. the proteolytic activity of v. vulnificus metalloprotease (vvp) toward casein and elastin was inhibited by alpha 2-macroglobulin (alpha 2 m) at the molar ratio of 1:1, although partial activity was maintained. permeability-enhancing and hemorrhagic activities were also inhibited, but the peptidase activity toward z-gly- ... | 1989 | 2478526 |
| differential immunohistochemical localization of cytokeratins and collagen types i and iii in experimentally-induced cirrhosis. | liver cirrhosis was induced in male wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. these results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepa ... | 1989 | 2478687 |
| sugar composition of the polysaccharide portion of lipopolysaccharides of vibrio fluvialis, vibrio vulnificus, and vibrio mimicus. | a chemotaxonomic study was carried out on vibrio fluvialis and v. vulnificus on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharides (lps). a previously developed rapid method of preparing samples for compositional sugar analysis was employed. nineteen o-serogroups of v. fluvialis were divided into 14 chemotypes while seven o-serogroups of v. vulnifucus were divided also into seven chemotypes since the polysaccharide portion of lps of each serogroup has a ... | 1989 | 2482433 |
| hemochromatosis and infection: alcohol and iron, oysters and sepsis. | hemochromatosis, or primary iron overload, is a variably expressed genetic metabolic disorder greatly modified by sex, age, diet, and alcohol consumption. although a diagnosis has been made at the bedside by careful documentation of the slow resolution of subcutaneous iron pigment, clinical diagnosis is frequently overlooked, and even autopsy may fail to reveal hemochromatosis as the cause for cirrhosis. genetic linkage studies have confirmed the extremely high prevalence of this disorder. untre ... | 1989 | 2486533 |
| immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen. | we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ... | 1989 | 2491623 |
| unproven methods of cancer management. international association of cancer victors and friends, inc. | after study of the literature and other available information, the american cancer society has found no evidence that methods of treatment promoted by the international association of cancer victors and friends, inc. (iacvf), would result in objective benefit in the treatment of cancer in human beings. lacking such evidence, the american cancer society strongly urges individuals with cancer not to seek treatment based on the recommendations of iacvf. | 1989 | 2492878 |
| hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis. | monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ... | 1989 | 2493084 |
| activation of hageman factor and prekallikrein and generation of kinin by various microbial proteinases. | activation of the hageman factor-kallikrein-kinin system by serratial 56-kda proteinase was previously demonstrated (matsumoto, k., yamamoto, t., kamata, t., and maeda, h. (1984) j. biochem. (tokyo) 96, 739-749; kamata, r., yamamoto, t., matsumoto, k., and maeda, h. (1985) infect. immun. 48, 747-753). to investigate whether the activation of the system is specific for 56-kda proteinase or is found similarly with other microbial proteinases, 11 proteinases of microbial origins were studied; the 5 ... | 1989 | 2499581 |
| [two case reports of septic shock due to vibrio vulnificus with liver cirrhosis]. | we have recently experienced a case of vibrio vulnificus septicemia which occurred in a patient with hepatic cirrhosis, and as we were able to give early antibiotic treatment, the patient survived. we would like to report this case here together with another case experienced 2 years ago. case 1 was a 58-year-old male who was attending our hospital as an outpatient for hepatic cirrhosis. at 5:30 pm on august 8, 1987, he consumed abalone and giant clam and at 9 pm complained of high fever with sha ... | 1989 | 2501432 |
| killing of human tumor cells by antibody c3b conjugates and human complement. | to potentiate the lytic action of complement on tumor cells, we have constructed heteroconjugates composed of monoclonal antibody and of the human c3b component of complement. the conjugates were formed efficiently using the heterobifunctional cross-linking reagent spdp. the monoclonal antibody-c3b conjugate promoted the killing of k562 tumor cells by normal human serum. treatment of the tumor cells with the monoclonal antibody and normal human serum resulted in 10-15% lysis. however, following ... | 1989 | 2519543 |
| cell proliferation kinetics of epidermis, hair follicles, and sebaceous glands of cocker spaniels with idiopathic seborrhea. | cell proliferation kinetic values were established for the epidermis, hair follicle epithelium, and sebaceous glands of 8 cocker spaniels with primary idiopathic seborrhea. values were established by intradermal pulse labeling injections of tritiated thymidine followed by cutaneous biopsy and autoradiography. the epidermal basal cell-labeling index was 4.96 +/- 0.97%, and the epidermal nucleated cell-labeling index was 3.33 +/- 0.71%. calculated epidermal cell renewal time for the viable layers ... | 1989 | 2533468 |
| bile acid profile in a dog with cholangiocarcinoma. | | 1989 | 2536512 |
| a simple colorimetric assay method for pyrophosphate in the presence of a 1000-fold excess of orthophosphate: application of the method to the study of pyrophosphate metabolism in mitochondria. | a sensitive colorimetric method for the assay of inorganic pyrophosphate with excess of orthophosphate is described. the principle of this method lies in the formation of phosphomolybdate and ppi-molybdate complexes with subsequent extraction of the phosphomolybdate complex by organic solvents and reduction of the ppi-molybdate complex by dithiothreitol and eikonogen. the sensitivity of the method was from 5 to 120 nmol of ppi in a 2.0-ml sample. | 1989 | 2545112 |
| sodium and potassium currents recorded during an action potential. | a simple method was used to measure directly sodium and potassium currents underlying the action potential in single nerve fibres of xenopus laevis. a short rectangular stimulus under current-clamp conditions elicited an action potential which was digitally stored and later used as command when voltage-clamping the same fibre. the currents thus obtained nearly reproduced the original rectangular stimulus. adding first 100 nm ttx and subsequently 100 nm ttx plus 10 mm tea to the extracellular rin ... | 1989 | 2546753 |
| hypoxic changes in hippocampal neurons. | 1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ... | 1989 | 2547034 |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| comments on radiation-induced g2 arrest in mouse zygotes. | | 1989 | 2564879 |
| in-vivo effects of (e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine (mdl 72145) on amine oxidase activities in the rat. selective inhibition of semicarbazide-sensitive amine oxidase in vascular and brown adipose tissues. | one hour after mdl 72145 ((e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine) (2.5 mg kg-1) was given by intraperitoneal injection, the semicarbazide-sensitive amine oxidase (ssao) activity of rat aorta and brown adipose tissue measured in-vitro was reduced by more than 95% of its control value, whereas the monoamine oxidase (mao-a) activity remained virtually unaffected. the action of this drug on amine oxidases in the liver at this dose was less selective. the in-vitro effect of mdl 72145 on the ... | 1989 | 2565962 |
| effects of toxin isolated from the sea anemone bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes. | electrophysiological effects of a polypeptide toxin isolated from the sea anemone bolocera tuediae (bttx ii) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. nanomolar concentrations of bttx ii cause a concentration-dependent depolarization. this effect is prevented by the presence of tetrodotoxin. action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. the positive overshoot a ... | 1989 | 2566207 |
| detection of piluslike structures on clinical and environmental isolates of vibrio vulnificus. | twenty clinical isolates of vibrio vulnificus were compared with 10 environmental strains by using electron microscopy and agglutination assays with human erythrocytes, guinea pig erythrocytes, and saccharomyces cerevisiae. in addition, the isolates were tested for ability to adhere to the human epithelial cell lines hep-2 and a549. when examined by electron microscopy, 16 (80%) of the 20 clinical isolates demonstrated the presence of piluslike structures; the composition of the bacterial popula ... | 1989 | 2568368 |
| recombinant human erythropoietin treatment in patients on maintenance home haemodialysis. | | 1989 | 2570273 |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| characterization of rat cerebral cortical beta adrenoceptor subtypes using (-)-[125i]-iodocyanopindolol. | (-)-[125i]-iodocyanopindolol [-)icyp), used to characterize beta adrenoceptors on membrane preparations from rat cerebral cortex, was shown to have affinity for both beta adrenoceptors and serotonin receptors. therefore, 10 microm serotonin was added to the assays to prevent (-)icyp binding to serotonin receptors. under these conditions, (-)icyp binding to the cortical membrane preparation was reversible and saturable, and the association reaction was very slow. the dissociation reaction was als ... | 1989 | 2577008 |
| relation of capsular materials and colony opacity to virulence of vibrio vulnificus. | colonies which varied in opacity were isolated from the four strains of vibrio vulnificus. opaque and translucent colonial types of the strains were distinguished from the corresponding parent strains. variation in the opacity of colonies formed by each strain was accompanied by variation of capsular material formation, which was clarified by electron microscopy of the organisms stained with ruthenium red. the opaque-type colonies of the strains had capsular materials. on the other hand, three t ... | 1985 | 2578434 |
| intestinal infection with mycobacterium avium in acquired immune deficiency syndrome (aids). histological and clinical comparison with whipple's disease. | at endoscopy, a 30-year-old man with acquired immune deficiency syndrome (aids), kaposi's sarcoma, diarrhea, and unexplained malabsorption showed erythematous macular duodenal lesions consistent with whipple's disease by histology and electron microscopy. symptoms did not respond to tetracycline. subsequent cultures revealed systemic mycobacterium avium (m. avium) infection. tissue from this patient, from patients with whipple's disease and from a macaque with m. avium were compared. all contain ... | 1985 | 2580679 |
| [serologic studies of listeriosis antibodies in dogs in greece]. | in an epidemiological study of dogs carried out in greece, antibodies against listeriosis were demonstrated in the slow-agglutination and the complement-fixation-reaction. depending on the dogs' place of origin cfr titers were found in 6.1% to 51.1%. cfr-titers could be confirmed in a comparative investigation with the slow-agglutination-test. | 1989 | 2583499 |
| ultrastructure of the tail of echinostoma revolutum cercaria. | the ultrastructure of the tail fins and muscles of echinostoma revolutum cercaria is described. the longitudinal muscles consist of a primitive type of transversely striated muscle fibres with a well-developed sarcoplasmic reticulum in the z-band. the fins are formed by a longitudinal tegument fold containing the same components as the remaining part of the tail tegument. the difference between the body and tail tegument is discussed. | 1989 | 2583613 |
| pathological analyses on the compressed cord by the experimental spinal metastasis. | the purpose of the present experimental spinal metastasis developed in rats by inoculation of walker's cancer cells through the spinous process is to find the factor which causes the initial damage of the cord in this disorder. in the early stage of paralysis, microangiogram exhibited that the degenerated posterior funiculus originated from the small hemorrhagic area in the posterior column of the involved cord. scanning electron microscopic findings showed that the hemorrhage was of the intrins ... | 1989 | 2586714 |
| susceptibility of erythrocytes from several animal species to vibrio vulnificus hemolysin. | the hemolytic activity of vibrio vulnificus hemolysin (vvh) against erythrocytes from several animal species (sheep, horse, cow, rabbit, chicken) was investigated. vvh was active against erythrocytes from all species, but the amount of vvh causing 50% hemolysis under identical conditions (hemolytic susceptibility to vvh) differed. the degree of 125i-labeled vvh (125i-vvh) binding to each erythrocyte species correlated with the susceptibility of the cells to hemolysis. however, marked differences ... | 1989 | 2612874 |
| septicaemia due to vibrio vulnificus. | | 1989 | 2617638 |
| survival of vibrio vulnificus in shellstock and shucked oysters (crassostrea gigas and crassostrea virginica) and effects of isolation medium on recovery. | when two species of shellstock oysters were artificially contaminated with vibrio vulnificus, the bacterium survived when the oysters were stored at 10 degrees c and below. large numbers of endogenous v. vulnificus cells were found after 7 days at both 0.5 and 10 degrees c in uninoculated control oysters (crassostrea virginica). oysters allowed to take up v. vulnificus from seawater retained the bacterium for 14 days at 2 degrees c. the presence of v. vulnificus in the drip exuded from the shell ... | 1989 | 2619304 |
| [rational treatment of necrotizing external otitis]. | advances in the diagnosis and treatment of necrotizing external otitis (neo) during the past 20 years improved substantially the prognosis of this disease. the use of hyperbaric oxygen therapy greatly potentiates the effect of newly developed highly effective antibiotics. radioisotope examination of the petrous bones facilitates the diagnosis and indicates the time to terminate treatment. medicamentous treatment is preferable to surgical treatment, which is reserved for exceptional very advanced ... | 1989 | 2631997 |
| [rational treatment of necrotizing external otitis]. | advances in the diagnosis and treatment of necrotizing external otitis (neo) during the past 20 years improved substantially the prognosis of this disease. the use of hyperbaric oxygen therapy greatly potentiates the effect of newly developed highly effective antibiotics. radioisotope examination of the petrous bones facilitates the diagnosis and indicates the time to terminate treatment. medicamentous treatment is preferable to surgical treatment, which is reserved for exceptional very advanced ... | 1989 | 2631997 |
| studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1). | anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ... | 1989 | 2632089 |
| studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1). | anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ... | 1989 | 2632089 |
| myofibroblasts in cotton-induced granulation tissue and the bovine adrenal capsule: morphological aspects. | ultrastructural studies of one week old cotton-induced granulation tissue revealed the presence of polymorphonuclear neutrophils (pmn), macrophages and mast cells, whilst the granulation tissue from two week old cotton implants contained fibroblasts, myofibroblasts and abundant collagen fibres. similarly, the bovine adrenal capsule contained both fibroblasts and myofibroblasts. it is proposed that the bovine adrenal capsule and two week cotton-induced granulation tissue could be utilized as in-v ... | 1989 | 2634624 |
| short chain fatty acid-induced colitis in mice. | short-chain fatty acids (e.g. acetic, propionic and butyric acid) are metabolic products produced by colonic bacteria. since intra-rectal instillation of dilute acetic acid to rats and cats has been reported to produce a diffuse colitis similar to the human disease, in our study we have investigated the effect of intra-rectal butyric acid in mice. male bka mice (n = 6) received 01 ml of dilute butyric acid by intra-rectal instillation which was flushed out with saline after 10 sec. control anima ... | 1989 | 2634625 |
| redirection of prostaglandin endoperoxide metabolism at the platelet-vascular interface in man. | prostacyclin (pgi2) is an inhibitor of platelet function in vitro. we tested the hypothesis that pgi2 is formed in biologically active concentrations at the platelet-vascular interface in man and can be pharmacologically modulated to enhance its inhibitory properties. this became feasible when we developed a microquantitative technique that permits the measurement of eicosanoids in successive 40-microliters aliquots of whole blood emerging from a bleeding time wound. in 13 healthy volunteers the ... | 1989 | 2643628 |
| internalization and degradation of insulin by a human insulin receptor-v-ros hybrid in chinese hamster ovary cells. | chinese hamster ovary cell lines expressing either the wild-type human insulin receptor or a hybrid molecule in which the tyrosine kinase domain of the insulin receptor is replaced with that of the oncogene, v-ros were examined for their ability to internalize and degrade insulin. cells expressing the hybrid receptor were found to internalize and degrade insulin at approximately half the rate of cells expressing the native insulin receptor. moreover, insulin was incapable of inducing the interna ... | 1989 | 2645866 |
| therapeutic implications of tumor heterogeneity. | at the outset of this review, we stated that we wished to raise some questions that challenge the commonly held view that tumor heterogeneity is of major significance to treatment failure. the nature of this challenge is the following: although tumor heterogeneity in sensitivity to therapeutic agents has been demonstrated repeatedly, using isolated subpopulations of cells, primarily in cell culture systems, there is very little work that has been directed toward asking the tough questions about ... | 1989 | 2652316 |
| detection of vibrio vulnificus cytolysin in v. vulnificus-infected mice. | enzyme-linked immunosorbent assays using polyclonal and monoclonal antibodies specific for v. vulnificus cytolysin detected the toxin in an extract of skin lesions and in serum from mice showing local and systemic v. vulnificus disease after subcutaneous injection of the bacterium. the cytolysin also was detected in skin lesions by an indirect immunofluorescence procedure using polyclonal and monoclonal antibodies. our findings provide direct evidence that the cytolysin is produced in vivo durin ... | 1989 | 2658204 |
| pulsed-field gel electrophoresis of circular dna. | mobility of supercoiled (form i) and nicked circular (form ii) plasmid dnas was determined on two major forms of pulsed-field electrophoresis, chef and ofage. plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with saccharomyces cerevisiae chromosomes as standards. agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels resolving forms i and ii of smaller plasmids. the pulsing range of 3.7 to 240 seconds resulted in quite v ... | 1989 | 2662139 |
| review of cardiovascular findings in humans treated with minoxidil. | minoxidil is an antihypertensive vasodilator with no known capability of inducing human cardiovascular pathology; however, the compound does cause a late, canine-specific lesion of the right atrium and acute right atrial and ventricular papillary muscle changes. in all the autopsy cases of minoxidil-treated humans to date, 9 cases were reported with significant right atrial or other cardiac pathology and were carefully reviewed for similarity to either the early or late canine lesion. three new ... | 1989 | 2665032 |
| vibrio vulnificus infection in hawaii. | a life-threatening vibrio vulnificus infection occurred in a 52-year-old korean woman with hepatic cirrhosis. four days after ingesting raw crab, the patient presented to the hospital with nausea, vomiting, fever, hypotension, and hemorrhagic blistering of the left foot. vibrio vulnificus was recovered from both her blood and a foot wound. | 1989 | 2666323 |
| infectious and toxic syndromes from fish and shellfish consumption. a review. | primary care physicians care for large numbers of patients presenting with "food poisoning" or gastroenteritis. when a patient who presents with acute gastrointestinal illness, especially in conjunction with neurologic or cutaneous symptoms, is evaluated, the history should focus on past seafood consumption (particularly raw or undercooked seafood). the infectious syndromes are generally self-limited and respond to supportive care; exceptions are those caused by vibrio cholerae and vibrio vulnif ... | 1989 | 2669661 |
| a new procedure for the targeted inactivation of essential bacterial genes. | a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ... | 1989 | 2674660 |
| a new procedure for the targeted inactivation of essential bacterial genes. | a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ... | 1989 | 2674660 |
| a biotin-streptavidin amplified enzymeimmunoassay for 13,14-dihydro-15-keto-pgf2 alpha. | as an alternative for radioimmunoassays a new enzyme immunoassay (eia) for the determination of 13,14-dihydro-15-keto pgf2 alpha (pgfm) has been developed. biocytin was linked to pgfm by the n-hydroxysuccinimide method and the product (biocytinyl-pgfm) purified by reversed phase column chromatography. biocytinyl-pgfm was used in the eia as a bridge between the immobilized pgfm-antibody and streptavidin-peroxidase. the absolute sensitivity of the assay was about 160 amol (92% rel. binding) and re ... | 1989 | 2675197 |
| establishment and characterization of human glioblastoma cell lines in vitro and their xenografts in nude mice. | human gliomas were grown as permanent tissue cultures and xenografts in nude mice. growth kinetics, immunohistology and karyotypes were established. one tumor showed a distinct change in its karyotype after only two passages in nude mice; by contrast, no change of immunophenotype occurred during and after in vivo passaging. treatment of this glioma as xenograft in nude mice revealed a high sensitivity towards single agent treatment with bcnu. in view of the cytologic aberrations, however, this r ... | 1989 | 2677876 |
| digit-muscle responses evoked from multiple intracortical foci in monkey precentral motor cortex. | 1. the precentral motor cortex, including the anterior bank of the central sulcus of monkey (macaca fuscata), was systemically penetrated with microelectrodes to determine the spatial organization of the microexcitable cortical elements that can produce responses in digit muscles. 2. at 200-microns intervals on each electrode track, low-current intracortical microstimuli were delivered and the muscle responses evoked from four digit muscles were recorded. the responses, obtained with 5, 8, 15, a ... | 1989 | 2681562 |
| acute gluteal abscesses: injectable chloroquine as a cause. | one hundred and seven patients admitted to the casualty department with acute gluteal abscesses and surgically drained were studied. in 91 patients (85%) the abscess was a result of an intramuscular injection, of whom 71 patients (66%) had the abscess following a chloroquine injection (antimalarial). of those patients with an abscess following an intramuscular injection, in 64 patients (70%) a disposable syringe was used for injection of the drug and in 18% a boiled disposable was used. the inje ... | 1989 | 2681814 |
| phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-hiv activity. | oligodeoxynucleotides with a phosphorus atom in which one of the non-bridging oxygen atoms is substituted by selenium were prepared and investigated with respect to their antisense properties. a general synthesis of phosphoroselenoate analogs of oligonucleotides is described using potassium selenocyanate as the selenium donor. the compounds, characterized by 31p nmr, were shown to decompose to phosphate with a half-life of ca. 30 days. melting temperatures of duplexes between poly(ra) or poly(ri ... | 1989 | 2682524 |
| the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function. | a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ... | 1989 | 2685548 |
| the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function. | a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ... | 1989 | 2685548 |
| [principle, method, and application for the production of peptide hormones using genetic engineering]. | | 1989 | 2691727 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| vibrio vulnificus infection. | we report 3 cases of vibrio vulnificus infections from taiwan. patient 1, who manifested symptoms of primary septicemia, died after 2 days. patient 2, who had a wound infection and signs and symptoms of sepsis but negative blood cultures, responded to tobramycin and chloramphenicol plus surgical debridement, and recovered after 26 days of hospitalization. patient 3 had secondary septicemia originating from a wound inflicted by a shrimp. originally, the patient seemed to respond to ceftazidime an ... | 1989 | 2694352 |
| vibrio vulnificus septicaemia presenting as spontaneous necrotising cellulitis in a woman with hepatic cirrhosis. | vibrio vulnificus is a virulent marine organism commonly found in hong kong coastal waters which contaminates local sea-food. it may produce a primary septicaemia, often associated with secondary skin lesions, following ingestion of raw shell fish. we report a rapidly fatal case of primary v. vulnificus septicaemia in a 50-year-old housewife with post-hepatitic cirrhosis presenting as spontaneous necrotising cellulitis of the legs. v. vulnificus infection should be considered in patients with a ... | 1989 | 2694353 |
| parallels between neurasthenia and premenstrual syndrome. | neurasthenia and premenstrual syndrome became medical diseases because of the historical recognition of menstruation as a medical disease. both the nineteenth and twentieth century cultural views of women were important in the establishment of menstruation, neurasthenia and premenstrual syndrome as medical conditions. uncertainty of diagnosis with ever expanding diagnostic criteria, therapy undertaken without an adequate physiological basis, and often adverse effects from therapy, were character ... | 1989 | 2694626 |
| mechanism of lhrh-stimulated steroidogenesis in rat leydig cells: lipoxygenase products of arachidonic acid may not be involved. | luteinizing hormone releasing hormone agonist, [(imbzl)-dhis6,pro9,net]-lhrh (lhrh-a), caused a two to threefold increase in in vitro testosterone (t) secretion by rat leydig cells. this lhrh-a-induced t secretion was completely blocked by quinacrine and chloroquine, inhibitors of phospholipase a2. addition of phospholipase a2, however, was ineffective in stimulating basal or lhrh-a-induced t secretion. phospholipase c, on the other hand, significantly stimulated both basal and lhrh-a-induced t ... | 1989 | 2695506 |
| membrane fatty acid and virulence changes in the viable but nonculturable state of vibrio vulnificus. | the nonculturable state of vibrio vulnificus and, for comparison, that of escherichia coli were studied in artificial-seawater microcosms at 5 degrees c. total cell counts were monitored by acridine orange epifluorescence, metabolic activity by direct viable counts, and culturability by plate counts on selective and nonselective media. whereas total counts remained constant, plate counts of v. vulnificus suggested nonculturability by day 24. in contrast, direct viable counts indicated significan ... | 1989 | 2696428 |
| vibrio vulnificus sepsis after eating raw oysters. | vibrio vulnificus, a marine vibrio found in coastal waters of the united states, may contaminate certain seafoods, particularly raw oysters. patients with underlying liver disease are particularly susceptible to severe illness. unexplained febrile diseases in patients who have eaten raw oysters may be caused by vibrio vulnificus. a fatal and a nonfatal case are reported in two such patients. these patients are the first two reported cases of vibrio vulnificus infection in kentucky. | 1989 | 2723530 |
| characterization and purification of h1tf2, a novel ccaat-binding protein that interacts with a histone h1 subtype-specific consensus element. | definition of mechanisms regulating human histone h1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. two distinct binding activities have been identified in nuclear extracts from hela cells and mapped within a 180-base-pair (bp) region of a cell cycle-regulated h1 gene promoter. h1tf1 bound to an h1-specific a + c-rich sequence (ac box), 100 bp upstream of the cap site; h1tf2 inter ... | 1989 | 2725515 |