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isolation of cryptic plasmid deoxyribonucleic acid from kanagawa-positive strains of vibrio parahaemolyticus.twelve strains of vibrio parahaemolyticus was examined for plasmid deoxyribonucleic acid (dna) by dye-buoyant gradient centrifugation. four kanagawa-positive strains, all isolated from the same outbreak of gastroenteritis, contained multiple plasmid species of cryptic function. however, three kanagawa-negative strains and five kanagawa-positive strains were not found to contain demonstrable plasmid dna. r-plasmids were successfully transferred from escherichia coli to v. parahaemolyticus.1977326675
waterborne outbreaks. 1977328941
[change in the biological properties of salmonellae in acquiring resistance to chloramphenicol].mutants of salmonella typhimurium and salmonella abony resistant to 40 microgram/ml of chloramphenicol were obtained during selection according to the method of szybalski on hottinger broth with increasing concentrations of the antibiotic. by the colony morphology the mutants were divided into 4 groups. the study of the mutant biological properties revealed changes in the growth rate characterized by elongation of the lag-phase and exponential phase, changes in the biochemical activity evident f ...1979380452
a simple selective medium for isolation of vibrios with particular reference to vibrio parahaemolyticus. 1977598910
salt-starch xylose lysine deoxycholate agar. a single medium for the isolation of sodium and non-sodium dependent enteric gram-negative bacilli.salt dependent gram-negative bacilli responsible for gastroenteritis and tissue infections are often not recovered because proper media for isolation are not used. a salt-starch xld agar with 1.5% nac1 and 0.5% starch medium has been found to permit the isolation of pathogenic enterobacteriaceae, non-enterobacteriaceae gram-negative bacilli, and salt-dependent gram-negative bacilli, among which is vibrio parahemolyticus. as far as the enterobacteriaceae are concerned, the selectivity and sensiti ...1977604729
estimation of the incidence of halophilic vibrios--vibrio parahaemolyticus and vibrio alginolyticus--in the water and fauna of the black sea: possible relationship with the diarrheic disease. 1979401009
helical structure of the human umbilical cord.the helical structure of the human umbilical cord has been studied on 528 full-term cords from normal deliveries, 18 cords from aborted and developmentally normal fetuses with a cr length of 1.2-19.5 cm, 10 cords from monozygotic twins. in order to resolve the discrepancy in the nomenclature of the twist direction, a method has been suggested which takes into account the direction to which the fetus must have rotated to produce the twist. the incidence of the right helical pattern was 64.58%, of ...1979419931
[isolation of vibrio parahaemoliticus in residual waters]. 1977613445
first isolation in sri lanka of vibrion parahaemolyticus from a case of gastro-enteritis. 1977614098
vibrio parahaemolyticus as a cause of epidemic gastroenteritis. 1977615134
vibrio parahaemolyticus gastroenteritis outbreaks aboard two cruise ships.outbreaks of vibrio parahaemolyticus gastrointestinal illness occurred on two caribbean cruise ships in late 1974 and early 1975. in all, 697 passengers and 27 crew were affected. epidemiologic evidence incriminated seafoods served on the ships as the vehicles of transmission. the seafoods were probably contaminated by v. parahaemolyticus after cooking in seawater from the ships' internal seawater distribution systems. use of seawater in foodhandling areas was discontinued, and no further outbre ...1979433918
flavobacterium meningosepticum infections. 1979451641
some interesting isolates from a diagnostic laboratory.citrobacter koseri, plesiomonas shigelloides, edwardsiella tarda, yersinia enterocolitica, alkalescens dispar, vibrio parahaemolyticus, and vibrio alginolyticus were seven interesting microorganisms isolated recently in our diagnostic laboratory.1978641196
ultrastructure of thyroid follicular cells following hypophysial portal vascular infusion of thyrotropin-releasing hormone. 1978414615
travelers' diarrhea. 1978661919
[new k-type antigen of vibrio parahaemolyticus (author's transl)]. 1978671744
modulation of erythrocyte membrane proteins by membrane cholesterol and lipid fluidity.human erythrocyte membranes were enriched or depleted of cholesterol and effects on membrane proteins assessed with a membrane-impermeant sulfhydryl reagent, [35s]glutathione-maleimide. reaction of the probe with intact cells quantifies exofacial sulfhydryl groups and reaction with leaky ghost membranes permits quantification of endofacial sulfhydryl groups. the mean endofacial sulfhydryl titer of cholesterol-enriched membranes exceeded that of cholesterol-depleted membrane by approximately 45 n ...1979420782
[bathing water vibrio parahaemolyticus infections]. 1979473404
inhibition by polyanions of adherence by kanagawa-positive vibrio parahaemolyticus: a physicochemical effect.rapid adherence of kanagawa-positive vibrio parahaemolyticus to human fetal intestinal cells has been demonstrated in monolayer cultures. in this study, polyanionic carbohydrates and glycoproteins of various biochemical compositions, structural configurations, and molecular weights were found to inhibit this adherence. mono- and disaccharide components of inhibitory compounds did not themselves inhibit adherence. levels of ca++ in the test mixture were similar in both the presence and the absenc ...1979458197
[isolation of type ii vibrio parahemolyticus (v. alginolyticus)]. 1979461883
local tetanus in rats; concentration of amino acids as studied in spinal cord segments, spinal roots, and dorsal root ganglia.1. the effect of tetanus toxin injected into one gastrocnemius muscle on the steady state concentration of several amino acids was investigated in spinal cord half segments, spinal roots and dorsal root ganglia of rats. care was taken to ensure a symmetrical afferent input to the spinal cord and to localize the segment with the highest concentration of tetanus toxin. 2. in the spinal cord segments containing the highest concentration of tetanus toxin the steady state concentration of glycine was ...1979492350
investigation of an intracellular hemolytic agent of vibrio parahaemolyticus. lipid fraction of dried cells.the dried cells of two strains of vibrio parahaemolyticus were fractionated by extracting first with water and then with organic solvents. the hemolytic activity of the fractions was determined, and some of them were assayed for their effect in mice. the hemolytic agent present in the water-insoluble fraction was extractable in organic solvents such as 70% aqueous ethanol, chloroform-methanol-water (1:2:0.8) and acetone. the extracts showed no toxic effect in mice after intraperitoneal inoculati ...1979502896
[various biological impurities in food products of marine origin]. 1979542776
the utility of accelerated thermal degradation test for predicting the stability of freeze dried parahaemolytic vibrios. 1979546369
fly borne vibrio parahaemolyticus in calcutta.v. parahaemolyticus has been detected from flies in all months of the year in calcutta. the mean percentage of isolation is: housefly 14.1, blue bottle fly 18.5, external washing 10.1, viscera 22.6, flies of sweet shops 9.7 and those from fish market 22.7. flies revealed higher (4.4%) isolation of kanagawa postive v. parahaemolyticus strains than those of water and fish specimens (0.8%) studied previously. strains negative to kanagawa test but simulating enteropathogenic serotypes are recorded i ...1978749287
vibrio parahaemolyticus isolated from blood cultures. 1978751192
[problems of epidemiology of acute gastroenteritis caused by the parahemolytic vibrio]. 1975766531
[food poisoning by vibrio parahaemolyticus]. 1975767238
canine encephalitozoonosis in kennels and the isolation of encephalitozoon in tissue culture.the protozoan encephalitozoon was isolated in primary kidney cultures from dogs originating from three separate outbreaks of encephalitozoonosis in kennels. the disease was characterized by a fading syndrome in young puppies with nervous signs developing in some cases. it was not possible to reproduce the clinical disease with cultured organisms in either normal dogs or dogs immunosuppressed with methylprednisolone. the organisms were, however, reisolated in primary culture from two immunosuppre ...1979551201
stopping of the spontaneous beating of mouse and rat myocardial cells in vitro by a toxin from vibrio parahaemolyticus. 1977559094
incidence of salmonella spp., clostridium botulinum, and vibrio parahaemolyticus in an estuary.a study of the incidence of salmonella spp., vibrio parahaemolyticus-like organisms, and clostridium botulinum in samples collected at five stations located in the upper chesapeake bay, a major estuary on the atlantic coast of the united states, was conducted in december 1973 through december 1974. c. botulinum types b and e were detected in 12.3% of the total sediment samples examined. v. parahaemolyticus was recovered from 10.4% of a total of 86 water, sediment, and suspended sediment samples. ...1976776085
bacterial enteropathogens in malaysian shellfish.three species of commonly eaten shellfish found in malaysian coastal waters were examined for the presence of common bacterial enteropathogens. vibrio parahaemolyticus, non-agglutinating vibrios, and various serotypes of enteropathogenic e. coli were isolated from a large proportion of them. salmonella were isolated in two instances. high colony counts with evidence of faecal contamination indicated the strong possibility of pulltion being the cause for the presence of these enteropathogens. met ...1976788266
travelers' diarrhea: new developments. 1976790143
newer microbial agents in diarrhea. 1976797652
vibrio parahaemolyticus: aetiological agent of food poisoning.after mentioning the principal morphological, cultural, physiological, biochemical, enzyme and serological characteristics of vibrio parahaemolyticus (a facultative halophilic bacterium and the aetiological agent of food poisoning caused by the consumption of fish products) a description is given of its ecological aspects, geographical distribution, and potential enteropathogenicity for man, with general information on its isolation and identification.1976798718
[biological activity and receptor of thermostable direct hemolysin of vibrio parahaemolyticus (author's transl)]. 1976800273
[production of enteropathogenic toxin by vibrio parahaemolyticus and its thermostability. 1. quantitative analysis of the toxin and conditions for its production]. 1975815497
[production of enteropathogenic toxin by vibrio parahaemolyticus and its thermostability. 2. effects of heat on biological activity and immunological and physico-chemical properties of the toxin]. 1975815498
survival of bacteria in seawater using a diffusion chamber apparatus in situ.a microbiological survival chamber for in situ environmental studies involving microorganisms of public health significance was developed. the autoclavable chamber was provided with a supportive base for proper flow orientation and a battery-powered stirring mechanism for continuous internal agitation. the performance of the chamber and its ancillary units was evaluated in estuarine waters by diffusion studies and survival tests with eight species of bacteria isolated from environmental sources. ...1976820259
[effects of detergents on flagellation of vibrio parahaemolyticus]. 1977559791
[clinical features of patients suffering from food poisoning due to vibrio parahaemolyticus - with special reference to changes in electrocardiograms]. 1976824388
[new k antigen strain of vibrio parahaemolyticus]. 1977559793
hyperproduction of extracellular protease in mutants of vibrio parahaemolyticus. 1979502899
2,3,7,8-tetrachlorodibenzo-p-dioxin: covalent binding of reactive metabolic intermediates principally to protein in vitro.2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) is metabolized by the mouse liver cytochrome p-450-mediated monooxygenase system to reactive intermediates which bind 'covalently' to cellular macromolecules. although very difficult to quantitate, the presumably covalent binding to microsomal protein occurs between 120 and 2,640 times more readily than binding to deproteinized dna in the in vitro reaction. because of the extremely high rate of binding to protein rather than to dna, it is visualized tha ...1979515166
effect of carbohydrate source on kanagawa-type hemolysis of vibrio parahaemolyticus.almost all strains of vibrio parahaemolyticus produced kanagawa-type hemolysis on media of high salt content in the presence of fermentable carbohydrates.1977853123
large animal hepatotoxic and nephrotoxic plants.the hepatotoxic and nephrotoxic plants of large domestic animals have been reviewed. the most important ones are those widely distributed as weeds over pastures, negelcted forests and grasslands, those used as ornamentals, the nitrate concentrating forage crops, and the cyanophoric plants. crotolaria spp, the ragwort (senecia jacobaea), the lantana spp. and heliotopum are common hepatoxic plants. amaranthus retroflexus, datura stramonium, solanum rostratum, and the castor oil plant (ricinus comm ...1979516370
distribution of vibrios in calcutta environment with particular reference to v. parahaemolyticus. 1977863485
the distribution of vibrio parahaemolyticus in british coastal waters: report of a collaborative study 1975--6.a collaborative survey has shown that v. parahaemolyticus is widely distributed in british coastal waters, sediments and shellfish, especially in southern and western areas. the relatively small numbers in the environment do not present significant health hazards from marine products, provided that processing, storage and distribution are adequate. the presence of this organism in small numbers in british coastal waters or in shellfish should not in itself be regarded as cause for concern.1978632568
the nucleus of the basal optic root in the pigeon: an electron microscope study.the ultrastructure of the nucleus of the basal optic root in an avian species (columba livia) was investigated. the ectomamillary nucleus (emn) in which terminates the basal optic tract reveals three types of neurons: 1) small round neurons bearing a scanty cytoplasm in organelles, 2) medium-sized neurons, spindle-shaped with a dense population of organelles and 3) large multipolar neurons with well developed perikaryal elements. some of these neurons have their inner plasma-membrane which fuse ...1979526019
reverse transformation of chinese hamster ovary cells by a factor produced by vibrio parahaemolyticus. 1977875243
growth of heat-injured vibrio parahaemolyticus in media supplemented with various cations.mid- to late logarithmic growth phase cells of vibrio parahaemolyticus grown in tryptic soy broth (tsb) containing 0.5, 3.0, and 7.5% nacl were heated for 8 min at 45 degrees c in 0.1 m phosphate buffer (ph 7.2) containing 3% nacl. colony formation on thiosulfate-citrate-bile salts-sucrose agar (tcbs) containing 2% nacl was greatest for unheated cells that had been grown in 7.5% nacl-tsb; cells grown in 0.5% nacl-tsb formed a greater number of colonies on 1.0% nacl-tcbs. thermal injury was evide ...1977879768
toxin isolation from a kanagawa-phenomenon negative strain of vibrio parahaemolyticus.production of a toxin by vibrio parahaemolyticus kanagawa-phenomenon negative strains was examined. ammonium sulfate fractions of broth culture filtrates were dialyzed, concentrated by lyophilization, and tested for toxic effects by mouse intraperitoneal injection. one fraction, which we think is a toxin, was isolated from a broth culture filtrate of v. parahaemolyticus fc 1011 (a kanagawa-phenomenon negative strain) and consitently produced lethal effects in mice at high concentrations and diar ...1977887001
wound infections in australia from halophilic vibrios.halophilic vibrios were isolated from extra-intestinal sites of two patients. the organisms isolated, both apparently of marine origin, were probably pathogenic at their respective sites. it is suggested that extra-intestinal infection caused by halophilic vibrios may be more common than reported, and may be more easily demonstrated by the use of the analytab products inc. (aip) system.1977887020
[lethal activities of the thermostable direct hemolysin produced by vibrio parahaemolyticus (author's transl)]. 1977606853
in vitro response to chloramphenicol, tetracycline, ampicillin, gentamicin, and beta-lactamase production by halophilic vibrios from human and environmental sources.isolates of vibrio parahaemolyticus and v. alginolyticus from human and environmental sources were examined for antibiotic susceptibility by the methods of minimal inhibitory concentration (mic) in broth and agar diffusion. these strains were found to be almost uniformly susceptible to chloramphenicol and tetracycline within attainable serum levels. the relationships of zone sizes to mics for these two antimicrobial agents and ampicillin conformed essentially to those obtained by standard method ...1978646346
[presence and etio-epidemiological significance of halophilic vibriones in various material].in this work data of isolation of halophilic vibrios from coastal water of central tyrrhenian sea are reported, and the epidemiological significance of such strains is discussed. v. parahaemolyticus was identified in 8 out of 20 samples of sea water. v. alginolyticus was identified in 8 out of 26 samples of fishes and mussels examined and in 8 out of 20 sea water samples. halophilic vibrios had not been isolated from 12 samples of sewage water and from 27 healthy or pathological human stool samp ...1977901634
[isolation of halophilic vibrios as possible agents of food poisoning (author's transl)].seventy-six halophilic vibrio strains were isolated from different marine sources such as coastal waters, fishes and mussels. twenty strains were identified as vibrio parahaemolyticus, 44 as vibrio alginolyticus and 12 other strains could not be classified because of their atypical biochemical activities. further investigations are necessary in order to assess if the present strains of v. parahaemolyticus can have a role in food poisoning outbreaks or human sporadic enteritis in our geographic a ...1977607885
vibrio cholerae, vibrio parahaemolyticus, and other vibrios: occurrence and distribution in chesapeake bay.vibrio cholerae was isolated at several locations in chesapeake bay in fall 1976 and spring 1977. strains induced fluid accumulation in rabbit ileal loops and positive activity in y-1 adrenal cells. vibrio cholerae, vibrio parahaemolyticus, and related vibrios show a spatial and temporal distribution characteristic of vibrio species in an estuary. the vibrio cholerae strains isolated from chesapeake bay represent serotypes other than o-group i--that is, so-called nonagglutinable vibrios--and are ...1977910135
[crab cocktail and vibrio. another source of diarrhea in travelers]. 1977917132
[an explosion of food poisoning among a group of travelers]. 1977917134
antibiotic sensitivity of v. parahaemolyticus from cases of gastroenteritis. 1977924560
vibrio parahaemolyticus infection in man. 1977924591
[a simple method for kanagawa-test of vibrio parahaemolyticus by a liquid medium (author's transl)]. 1977926306
antimetabolite sensitivity and magnesium uptake by thermally stressed vibrio parahaemolyticus.metabolic inhibitors were added to a culture medium inoculated with theramlly stressed vibrio parahaemolyticus to obtain information pertaining to biosynthetic processes required for recovery from heat damage. ribonucleic acid and protein syntheses, in addition to membrane repair, were required during recovery of injured cells. neither nucleic acid nor mg2+ leakage was noted to occur during the time cells were subjected to heat stress. studies revealed that mg2+ was apparently taken up by cells ...1978677870
vibrio parahaemolyticus enterocolitis in bangladesh: report of an outbreak.in march 1974 eight men in chandpur, bangladesh, experienced an illness characterized by severe abdominal cramps, nausea, vomiting and bloody diarrhea with onset 20 min to 9 h (median 2.5 h) after eating one of two fish dishes at a restaurant. rectal cultures from all eight grew kanagawa-positive strains of vibrio parahaemolyticus (serotype o3k5) that were negative in the sereny test for invasiveness and the y-1 adrenal cell and infant mouse assays for enterotoxin production. the short incubatio ...1978626266
food poisoning due to vibrio parahaemolyticus. 1977580237
relationships between heat resistance and phospholipid fatty acid composition of vibrio parahaemolyticus.vibrio parahaemolyticus was grown in tryptic soy broth (tsb) containing nacl levels of 0.5, 3.0, and 7.5% (wt/vol). cultures incubated at 21, 29, and 37 c were harvested in late exponential phases and thermal death times at 47 c (d47 c; time at 47 c required to reduce the viable population by 90%) were determined in phosphate buffer containing 0.5, 3.0, and 7.5% nacl. at a given nacl concentration in the growth medium, d47 c values increased with elevated incubation temperatures and with elevate ...1976938034
ultraviolet sensitivity of vibrio parahaemolyticus, a causative bacterium of food poisoning. 1977594178
[isolation of vibrio parahemolyticus from mussel varieties in argentina]. 1977594428
fulminating lactose-positive vibrio septicemia.recently cases of tissue invasion by as yet unnamed marine vibrios which were morphologically and biochemically similar to both vibrio parahemolyticus and v. alginolyticus, but not identical with either of them, have been described. we have seen a patient who had serious widespread tissue infection with a halophilic, gram-negative bacterium which was isolated from blood and leg lesions. the organism had the characteristics of the genus vibrio, and lactose fermentation and onpg reactions were pos ...1978735824
vibrio parahaemolyticus: suspicion of presence based on aberrant biochemical and morphological features.vibrio parahaemolyticus was isolated from a stool specimen of a patient who developed gastroenteritis after ingestion of crab meat. recognition and identification of this halophilic microorganism was facilitated by the microscopic observation of a darting, vibrant motility in condensate derived from kligler iron agar and the bizarre morphological aberrations noted in the condensate obtained from christensen urea agar. sodium chloride supplementation (1.1%) of biochemical test media revealed the ...1978744803
incidence of vibrio parahaemolyticus bacteriophages and other vibrio bacteriophages in marine samples.vibrio bacteriophages were isolated by enrichment from 177 of 643 samples of marine molluscan shellfish, crustaceans, seawater, and sediments. the predominant bacteriophage types isolated were specific for some strains of vibrio parahaemolyticus. a high frequency of phage isolations was also observed with strains of agar-digesting vibrios (21 of 56) and psychrophilic vibrios (14 of 72) that were originally isolated from non-shellfish growing areas. no bacteriophages were isolated against v. algi ...1978727781
isolation of vibrio parahaemolyticus from persian gulf.we have been able to establish, for the first time, the presence of v. parahaemolyticus in the waters of the persian gulf. samples were collected from water, fish, shrimp, sand and sea vegetation around various coastal towns of the persian gulf during november 1977--april 1978. various biochemical tests have confirmed the identification of an isolate from one of the water samples as v. parahaemolyticus.1978748571
effect of dissolved aromatic hydrocarbons on the growth of marine bacteria in batch culture.dissolved aromatic hydrocarbons were found to decrease growth rate and maximum cell density of marine bacteria in batch cultures. the magnitude of the decrement was observed to be a function of concentration of the hydrocarbon and inherent toxicity. the inherent toxicity was observed to increase inversely with solubility such that naphthalene at 100 mum concentration demonstrated a toxic effect similar to benzopyrene at 0.02 mum. a partial oxidation product of naphthalene was found to be more ef ...1976970939
ecological relationship between vibrio parahaemolyticus and agar-digesting vibrios as evidenced by bacteriophage susceptibility patterns.twenty bacteriophages active against vibrio parahaemolyticus and agar-digesting vibrios, isolated from oysters (crassostrea gigas) and dungeness crab (cancer magister) and by induction of a lysogenic agar digester, were tested as to their host range. these phages were specific for v. parahaemolyticus and various agar-digesting vibrios, and interspecies lysis occurred only between these two groups. v. alginolyticus, v. anguillarum and related species, v. cholerae, and a group of marine psychrophi ...1978727782
[ecology of parahemolytic vibrions]. 1978732608
travelers' diarrhea in mexico. a prospective study of physicians and family members attending a congress.we conducted a prospective study of travelers' diarrhea on 73 physicians and 48 family members attending a medical congress in mexico city, in october, 1974. fecal and blood specimens were collected before, during and after their visit and examined for enteric bacterial pathogens, viruses and parasites. in 59 (49 per cent) participants travelers' diarrhea developed. median duration of illness was five days. onset occurred a median of six days after arrival. an etiologic agent was found in 63 per ...1976772435
isolation of an enteropathogenic, kanagawa-positive strain of vibrio parahaemolyticus from seafood implicated in acute gastroenteritis.a strain of vibrio parahaemolyticus, serotype o4/k4, that was kanagawa positive and reactive in the rabbit ileal loop test, was isolated in low numbers from raw oysters implicated in a case of acute gastroenteritis. to our knowledge, this is the first report of the isolation of a kanagawa-positive strain from suspect food in the united states.1978677883
[the dehydrogenation of butandiol by "vibrio parahaemolyticus" and other vibrios (author's transl)].the butandiol dehydrogenase was examined in 64 vibrios isolated in togo 1970/71. this enzyme was found in all of the not halophilic strains and in the species vibrio alginolyticus, whereas the species v. parahaemolyticus exhibited as well dehydrating as not dehydrating strains. the butandiol dehydrogenase could be a good marker for epidemiologic analysis in characterizing v. parahaemolyticus strains isolated from different sources, especially if no antisera are available.1976952445
vibrio parahaemolyticus: a cause of foodborne illness. 19761002591
marine vibrios associated with superficial septic lesions.three cases are reported in which a marine vibrio, vibrio alginolyticus, was isolated from superficial septic lesions. all cases had been exposed to sea-water. the possible significane of these findings and the need for further investigations are discussed.19761002833
[sensitivity of vibrio parahaemolyticus to antibacterial preparations]. 1976961231
sublethal heat stress of vibrio parahaemolyticus.when vibrio parahaemolyticsu atcc 17802 was heated at 41 degrees c for 30 min in 100 mm phosphate-3% nacl buffer (ph 7.0), the plate counts obtained when using trypticase soy agar containing 0.25% added nacl (0.25 tsas) were nearly 99.9% higher than plate counts using trypticase soy agar containing 5.5% added nacl (5.5 tsas). a similar result was obtained when cells of v. parahaemolyticus were grown in a glucose salts medium (gsm) and heated at 45 degrees c. the injured cells recovered salt tole ...19761008556
chick embryo virulence of some non-agglutinable (nag) vibrios. 19761008692
formation and function of vibrio parahaemolyticus lateral flagella.formation of the lateral flagella (l-flagella) of vibrio parahaemolyticus was studied immunologically, using specific antiserum against l-flagella. on solid medium, l-flagella were formed at both high (37 degrees c) and low (25 degrees c) temperatures, although at high temperatures they became dissociated from the cells and decomposed in the medium. l-flagella were not formed in liquid or soft-agar medium. formation of l-flagella was decreased by lowering the ph of the medium and repressed by tr ...1977845115
studies of the cell envelope of vibrio para-haemolyticus a55: isolation and purification of bag-shaped peptidoglycan (murein sacculus).the peptidoglycan (pg) component of the envelope of vibrio parahaemolyticus a55 was studied in relation to the salt dependency of the organism. a bag-shaped pg (murein sacculus) was isolated and purified by digestion of freshly harvested intact whole cells with sodium dodecyl sulfate (sds) and then protease treatment. whole cells lyze in salt-deficient or hypotonic conditions and this can be measured by turbidometry. lysis is prevented by addition of 0.35 m nacl or acidification to below ph 4.5. ...19761016218
isolation of a small rod with lytic activity against vibrio parahaemolyticus from fresh sea water.a small rod, capable of formine crater-like plaques on lawns of vibrio parahaemolyticus, was isolated from a marine environment. the isolate was a gram-negative straight rod with round ends and was small in size, equal to that of halophilic bdellovibrio strain 5501. the isolate appeared to have close taxonomic relationships to cytophaga, since this bacterium moved slowly in a gliding manner on a solid agar surface, hydrolyzed agar and starch, contained yellow pigment and was halophilic. the isol ...19761018344
vibrio parahaemolyticus and seafoods. 1976775645
[antibody titers against the thermostable direct hemolysin in the sera of patients with vibrio parahaemolyticus infection]. 1976820817
enteropathogenicity of vibrio parahaemolyticus in the ligated rabbit ileum.the enteropathogenicity of vibrio parahaemolyticus was investigated by contrasting the effects of whole cells, cell fragments, cell-free preparations, and media constituents injected into rabbit ileal loops. three of 20 cultures utilized were kanagawa-negative strains from seawater and sea fish. the remaining 17 cultures included both kanagawa-positive and -negative strains from japanese victims of gastroenteritis. broth culture filtrates concentrated 10-fold by dialysis against 30% carbowax wer ...1977836019
[vibrio parahaemolyticus in mussels and in silt in the southern central baltic sea (author's transl)].because the previous studies of 541 fish of the southern central baltic sea area gave negative results for the presence of vibrio parahaemolyticus, 150 shellfish and 40 mud samples of the same origin were tested this time. in none of them presence of vibrio parahaemolyticus nor vibrio alginolyticus has been stated, though.1977842182
sucrose teepol tellurite agar: a new selective indicator medium for isolation of vibrio species.sucrose teepol tellurite (stt) agar is a more successful medium in positive-recognition palting procedures than thiosulfate-citrate-bile salts-sucrose (tcbs) agar because stt agar yields higher isolation of typical colonies of vibrio cholerae, which directly agglutinate in antiserum to v. cholerae, from patients and contacts infected with this organism. stt agar has a simple composition and, like tcbs agar, needs no sterilization. stt agar is highly selective for v. cholerae, nonagglutinating vi ...1977856920
effect of hemolysin produced by vibrio parahaemolyticus on membrane conductance and mechanical tension of rabbit myocardium.the mechanisms of the action of hemolysin extracted from vibrio parahaemolyticus in the s-a node and right atrium cells of rabbit were studied by means of the single sucrose gap and isometric tension recording methods. hemolysin caused the membrane to depolarize reversibly without affecting the action potential generating mechanism. lowering of [na+]o inhibited membrane depolarization in the presence of hemolysin while the readmission of normal tyrode solution induced depolarization. tetrodotoxi ...1977864869
role of the cardiotoxin produced by vibrio parahaemolyticus in its infection. 1977875242
sublethal cold shock in vibrio parahaemolyticus.exposing vibrio parahaemolyticus populations to temperatures of 2 degrees c results in membrane damage, rendering cells incompetent to grow on media containing 5% nacl.1977889331
the isolation and characterization of some vibrio parahaemolyticus strains isolated from salted herring and roe.390 food samples (frozen, kippered herring, salted roe, fresh carp and salted ham) were investigated for the presence of vibrio parahaemolyticus. 8 v. parahaemolyticus strains were isolated from herring (6.2%) and roe samples (1.3%). some biochemical characteristics are discussed. in food borne outbreaks, presence of v. parahaemolyticus must be investigated, especially when the epidemiologically incriminated food is fish or fish products.1977899374
in vitro adherence of kanagawa-positive vibrio parahaemolyticus to epithelial cells.kanagawa-positive vibrio parahaemolyticus adhered rapidly to suspension-grown hela cells and to human fetal intestinal (hfi) cells in monolayer. kanagawa-negative v. parahaemolyticus did not adhere to hela cells and adhered to hfi cells at a much slower rate than kanagawa-positive bacteria. in the hfi cell system, adherence was dependent on the presence of calcium in the incubation mixture. it was unaffected by pretreatment of the hfi cell monolayer with neuraminidase or potassium periodate. adh ...1977908855
survival of vibrio parahaemolyticus after chilling in transport media: an explanation for divergent findings.differing incidences of vibrio parahaemolyticus reported by separate government agencies are attributable to sample handling and subsequent isolation techniques.1977931380
demonstration of agglutinating antibodies against vibrio parahaemolyticus in the yellow tail flounder (limanda ferruginea).sera from the salt water fish (yellow tail flounder) and the fresh water fish (rainbow trout) were tested for the presence of circulating antibodies against nine strains of vibrio parahaemolyticus and one of v. alginolyticus. thirteen out of 26 flounder sera tested gave agglutinating reactions against one specific strain of v. parahaemolyticus; strain no. 3525. seven rainbow trout sera tested failed to agglutinate any of the strains tested. all agglutinating activity was lost upon boiling the an ...1976931748
butandioldehydrogenase in vibrio parahaemolyticus.396 strains of v. parahaemolyticus, 345 originating from togo and 51 from japan, were submitted to the study of butandiol dehydrogenase (bdh). it was found that 52.3% of the strains were bdh-positive and 44.2% bdh-negative. an intermediate group characterized by weak reactions amounted to 3.5% of the strains. the distribution of this enzyme parallels to a certain degree the results of serotyping. positive reactions have been found in all or most strains belonging to serotypes 03k29, o4k8, 04k12, ...1976934022
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