| prophylactic antibiotics in cesarean section. | using a random selection procedure, 100 patients were evaluated in order to determine the value of prophylactic antibiotics in cesarean section. all patients were evaluated for febrile morbidity, and specific infection sites were recorded. significant differences were found between the treated and control groups and between cesarean sections performed prior to the onset of labor and after the onset of labor. | 1975 | 164642 |
| the acid mucopolysaccharides (glycosaminoglycans) in secondary cultures of embryonic rat fibroblasts. | in secondary cultures of embryonic rat fibroblasts the protein-free acid mucopolysaccharides were separated by cetylpyridinium chloride fractionation and identified afterwards. the following acid mucopolysaccharides were found: hyaluronic acid, chondroitin, 2 heparan sulphate fractions, chondroitin-4-sulphate, chondroitin-6-sulphate, and dermatan sulphate. | 1975 | 134903 |
| increased plasma prolactin levels induced in rats by d-fenfluramine: relation to central serotonergic stimulation. | d-fenfluramine (7.5 and 10 mg/kg i.p.) and quipazine (10 and 20 mg/kg i.p.) increased plasma prolactin levels in male rats. metergoline (3 mg/kg p.o.) or p-chlorophenylalanine (100 mg/kg x 3, orally) pretreatment markedly blocked the prolactin-releasing effect of both d-fenfluramine and quipazine. this result suggests that the effect of these drugs on prolactin secretion could be mediated through a serotonergic mechanism. brain serotonin may thus exert a stimulatory role on prolactin secretion i ... | 1978 | 149011 |
| pharmacological modification of experimental tardive dyskinesia. | cebus apella monkeys subjected to chronic haloperidol administration develop neurologic disturbances very similar to neuroleptic-induced acute dystonia human beings. after varying lengths of time, certain monkeys develop a prolonged dyskinetic syndrome resembling tardive dyskinesia (td), as seen clinically. two monkeys with signs of td were given single intramuscular injections of various compounds with known effects on the catecholaminergic, cholinergic, serotoninergic and gaba-ergic neurotrans ... | 1979 | 115227 |
| thyroid-stimulating hormone and cyclic adenosine 3',5'-monophosphate in the regulation of thyroid gland function. | the initial step in tsh action reflects binding of the hormone to specific receptor sites on the plasma membrane. such binding has been studied using plasma membranes, homogenates, isolated thyroid cells grown in culture, and thyroid slices. 3-h- and iodinated tsh preparations have been used; the latter have been prepared using both chloramine-t and lactoperoxidase. some of the discrepancies reported in the literature might reflect the different thyroid and hormone preparations and the variable ... | 1975 | 165359 |
| medical ethics and controlled clinical trials. | the controlled clinical trial is a relatively new phenomenon; the first large clinical trial on evaluation of streptomycin in therapy of pulmonary tuberculosis occurred in 1946. in such a study, two or more groups of patients with similar characteristics are chosen by random allocation to receive one or more therapies. the essential ethical dilemma is based on the risk-benefit ratio of the new therapy. ethical factors that must be considered in the design of controlled clinical trials include pr ... | 1979 | 115367 |
| letter: anergy in patients with syphilis. | | 1975 | 165417 |
| an in vitro chromosome assay using cultured rat-liver cells. | an in vitro chromosome assay has been developed which utilises an epithelial-like cell line derived from rat liver. the cell line, designed rl1, retains sufficient metabolic enzyme activity to detect chromosome damage induced by a variety of chemical mutagens and carcinogens without the incorporation of an extrinsic metabolising system. the cells are grown on standard glass microscope slides, exposed to the test chemical and processed in situ for metaphase analysis. in a small validation study, ... | 1979 | 117353 |
| ["in vitro" activity of a new cephalosporin (cgp-7174-e or cefsulodin) against "pseudomonas aeruginosa" (author's transl)]. | the minimal inhibitory concentration (mic) of four betalactamins (carbenicillin, ticarcillin, piperacillin and (cgp-7174-e) against 109 clinical isolates of pseudomonas aeruginosa was determined by an agar dilution method. cgp-7174-e was the most active (mic 3.4 micrograms/ml), followed in descending order by piperacillin (mic 4.9 micrograms/ml), ticarcillin (mic 22 micrograms/ml) and carbenicillin (mic 36 microgram/ml). high correlation was found between the mic of these betalactamins (r greate ... | 1979 | 120122 |
| reaction of yeast phosphofructokinase with succinic and maleic anhydride. | modification of yeast phosphofructokinase by succinic and maleic anhydride influences the catalytic activity and the allosteric behaviour of the enzyme. depending on the degree of succinylation and maleinylation a decrease of maximum activity, an increase of the apparent affinity for fructose-6-phosphate, a decrease of the hill-coefficient and a diminution of atp-inhibition are observed. up to about 40% of the lysyl residues could be succinylated without dissociation of the hexameric protein, ho ... | 1975 | 127493 |
| cardiac hypertrophy and antihypertensive therapy. | biochemical (myocardial dna, rna, and hydroxyproline) and humoral (plasma [pra] and kidney [kra] renin activity) factors were determined in spontaneously hypertensive rats (shr) and normotensive wistar controls (nr) before and following treatment with minoxidil or propranolol. minoxidil (150 mg.litre-1 drinking water) effectively controlled blood pressure (17.3 kpa vs 24.9 kpa [130 mmhg vs 187 mmhg], p less than 0.001) despite marked and sustained increases in both pra and kra ventricular weight ... | 1977 | 145319 |
| immunological methods employed in an attempt to induce erythema nodosum leprosum (enl) in mice. | in the mouse foot pad model five different parameters were employed to simulate the condition of enl as observed in the human. the experimental groups with five to six months leprosy infection were injected intravenously with various anti-mycobacterial antibodies, m. leprae sonicate and 'b' cells obtained from syngenic donors. the control group of animals, infected similarly, were treated either with m. leprae sonicate or gamma globulins precipitated from normal human serum. all recipients were ... | 1978 | 151764 |
| the use of nuclear medicine in evaluating liver disease. | | 1975 | 167451 |
| [changes in the lungs and the mass of the right ventricle of the heart in chronic hypoxia (experimental-morphological study)]. | in experiments on 105 white rats using histological, histochemical, and morphometric methods the state of the lungs following daily "rises" in a pressure chamber to the "hight" of 5000--9000 m at verious time intervals--from 1 day to 9 weeks was studied. in 18 animals "ascents" were preceded by sinistral pulmonectomy. in parallel, the heart was investigated by the method of separate weighing. at initial stages changes in the lungs were characterized predominantly by impairment of the blood- and ... | 1975 | 128341 |
| evaluation of different antibiotic actions combined with rifampicin. in vitro synergism against pseudomonas and proteus. | associations of penicillin, streptomycin, tetracycline and polymyxin b with rifampicin were tested for synergism against pseudomonas and proteus pathological strains. the combination of rifampicin and polymyxin b was clearly synergistic for all strains as it was evaluated by isobolograms and killing curvesmthe bactericidal action of this association, without any cross-resistance between its components, may eradicate gram-negative bacteria infections with marked polyvalent drug resistances. | 1975 | 169107 |
| detection of agents that alter the bacterial cell surface. | proteus mirabilis is not killed by polymyxin b, normal serum, or sodium deoxycholate. exposure to polymyxin b renders the cells susceptible to killing by the latter two agents. the data suggest that this synergism is due to polymyxin b-induced surface changes. the results point out an inadequacy of existing methods of screening for antibiotics; they fail to detect agents which, while showing no in vitro effect on growth, may alter a resistant organism so that it becomes more susceptible to other ... | 1975 | 169728 |
| immunization against experimental trypanosoma cruzi infection: inherent difficulties of a uniform comparative evaluation of antigenic preparations. | | 1979 | 120626 |
| [identification of camp dependant protein kinases in human lymphocytes]. | chromatographic purification by "deae" cellulose resolves the camp binding proteins in human lymphocytes into three parts. in presence of mg++ each one possesses camp dependent protein-kinase activity, one of them showing allosteric characteristics. | 1975 | 172248 |
| [resistance to association of antibiotics (polymyxin and penicillin) in relation to cell concentration of two bacterial species in the same inoculum. complementary inoculum effect]. | dense suspensions of sa. typhi (polys penr) and of st. aureus (polyr pens) proliferate on gelose medium added with the two antibiotics only if the inoculum is mixed. the growth is probably due to the partial neutralization of the antibiotics following the formation of complexes between these antibiotics and some constituents of the two strains. | 1975 | 172250 |
| [effects of caudate nucleus stimulations on the primary and secondary endings activity of soleus muscle spindle]. | effects of repetitive stimulation of the contralateral caudate nucleus on the static discharge and dynamic sensitivity of soleus muscle spindle primary and secondary endings were studied in cats anaesthetized with halothane (fluothane). by progressive increasing the depth of anaesthesia two different fusimotor effects could be observed. 1. a static facilitatory effect; under light anaesthesia the static discharge of primary and secondary endings were strongly increased. the response of the prima ... | 1975 | 123860 |
| comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. ii. the arthus reaction and the local shwartzman reaction. | the ability of antisera to guinea-pig c3 to inhibit the arthus and local shwartzman reactions was studied. they were found to reduce the non-haemorrhagic component of the active and reversed passive arthus reactions and to delay the appearance of the haemorrhage in the active arthus reaction. cobra venom factor, however, had no effect on the non-haemorrhagic components of these reactions and only delayed the appearance of the haemorrhage of the active arthus reaction. there appeared to be a corr ... | 1975 | 125318 |
| the effects of brain monoamine depletion on p-chlorophenyl-alanine-induced hypothermia. | i.p. administration of p-chlorophenylalanine produced a fall in rectal temperature in rats. the hypothermia was attenuated after pretreatment of the animals with 5,6-dihydroxytryptamine, but was unaffected after pretreatment of the animals with 6-hydroxydopamine. | 1978 | 153236 |
| persistence of cmv genome in lymphoid cells after congenital infection. | | 1975 | 172796 |
| irs, hew threaten triple-a bonds. | | 1978 | 152403 |
| chemotherapeutic nitroheterocycles. 18. 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups. | 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups and related compounds (41-69, table ii) were synthesized and their antimicrobial activities were evaluated (table iii). some of these compounds (e.g. 47, 52, and 59) suprisingly exhibited a broad antibacterial spectrum including proteus species and pseudomonas aeruginosa. extraordinary antitrichomonal activities could also be observed in vitro (mic of compound 59, 0.0004 pg/ml) and ... | 1975 | 166185 |
| interactions of heteroaromatic compounds with nucleic acids. 2. influence of substituents on the base and sequence specificity of intercalating ligands. | this paper presents the results of a systematic study on the effects of substituents on the base and sequence specificity of tricyclic heteroaromatic compounds interacting with dna by intercalation. all the compounds tested are derived from proflavine and acridine orange analogs with different heteroatoms in the middle ring. their base and sequence specificities were determined by differential dialysis of the ligand against dna samples of differing g-c content. the main results indicate that (a) ... | 1975 | 168079 |
| enhancement of adenovirus infection in wi-38 and agmk cells by pretreatment of cells with 5-iododeoxyuridine. | | 1975 | 166491 |
| therapeutic effect of beta-[1-phenyl-5-bis(beta-chloroethyl)-amino-benzimidazolyl-(2)-dl-alanin (zimet 3164) on immune complex nephritis of nzb hybrid mice. | beta-[1-phenyl-5-bis(beta-chloroethyl)-amino-benzimidazolyl-(2)]-dl-alanin (zimet 3164), a highly immunosuppressive but moderately cytostatic agent, was found to be able to prevent the progression of spontaneous murine immune complex nephritis in a dose-dependent fashion. for experiments, bcg-stimulated conventional female (nmri x nzb)f1 mice suffering from rapidly progressive nephritis, bcg-stimulated and unilaterally nephrectomized (nmri x nzb)f1 females, and (ab/jena x nzb)f1 females characte ... | 1979 | 161855 |
| [pathogenic and therapeutic concepts in viral hepatitis]. | | 1976 | 175890 |
| [pathomorphological changes in the estrogenic syndrome of swine]. | epizootic, clinical and pathomorphological investigations were carried out on spontaneously arising oestrogen syndrome in swine following feeding with fodder mixture containing 35-63% maize in which the oestrogen substance z-2 (zearalenon) was found. clinical symptoms and pathomorphological changes observed in the course of the disease are described. varying in extent edematous processes were present in the regions around the anus, vulva, prepucium and the mammal complex. uteruses were twice or ... | 1979 | 162016 |
| growth in semisolid agar medium of human cord leukocytes freshly transformed by epstein-barr virus. | the semisolid agar method for the selective growth of transformed cells was applied to investigations of epstein-barr virus (ebv) transformation. when 1 x 10(5) human umbilical-cord blood leukocytes were seeded in agar medium immediately after ebv exposure, about 100 colonies developed in each dish. the occurrence of colonies correlated well with dilutions of ebv inoculum. these colonies were composed of lymphoblasts, were positive for ebv-determined nuclear antigen immunofluorescence, and cons ... | 1976 | 176377 |
| [effect of fibrinolysin and heparin on the formation of fibrosis in experimental anthracosilicosis]. | | 1979 | 153880 |
| [experimental investigations concerning spectrum, serum concentration, and excretion of spectacillin (author's transl)]. | by means of the diffusion test, the range of action of the ampicillin successive preparation spectacillin was determined using 12 000 pathological germs freshly isolated from in-patients. the result was a range of action similar to that of ampicillin. a comparison, however, is only possible in case the test platelets (without over-dosage) are each charged with exactly, e.g., 25 gamma. the analyses of serum concentrations have shown that spectacillin is satisfactorily absorbed after oral as well ... | 1975 | 166292 |
| antibacterial activity of oxidized regenerated cellulose. | it has been demonstrated that oxidized regenerated cellulose promptly and markedly reduces the bacterial census of ten different strains of common pathogens when exposed to them in vitro. this was not true of two other hemostatic agents tested, namely, absorbable gelatin sponge and topical thrombin. wounds in guinea pigs in which oxidized regenerated cellulose was placed and infected with one of three pathogens had healing per primum in 39 of 40 instances. in similar control wounds and incisions ... | 1976 | 176740 |
| [the effect of standardised sodium hypochlorite solution on bacteria pathogenic for the urinary tract (author's transl)]. | stable sodium hypochlorite solution shows excellent disinfectant properties, even in higher dilution. parallel to clinical tests in vitro experiments were made to show the changes of morphology of bacteria pathogenic for the urinary tract by scanning microscope. two hours after incubation of the bacteria with sodium hypochlorite solution containing 40 mg available chlorine/1 severe damages of the bacterial morphology up to complete destruction were evident in all cases. gram-negative rod-shaped ... | 1976 | 176759 |
| metabolism, function and clinical aspects of vitamin d. | briefly reviewed herein are some of the contemporary findings on the metabolism of vitamin d, and the biochemical and physiological effects of this steroid in the animal. certainly the most accepted major action of vitamin d is to enhance the intestinal absorption of calcium. historically, there is also considerable evidence that the vitamin d is required for the resorption of calcium from bone, thereby aiding in maintaining normal serum calcium levels. increasing evidence is becoming available ... | 1975 | 163168 |
| evaluation of dye exclusion and colony inhibition techniques for detection of polyoma-specific, cell-mediated immunity. | cellular immunity directed against polyoma virus-induced antigen was observed with c3h/hej splenic lymphoid cells from mice sensitized by a short-term immunization schedule with syngeneic polyoma 4198 and 4198v tumor cells. polyoma specificity of the response was shown by demonstration that splenic cells from dba/2j animals with polyoma virus-induced tumors were cytotoxic for the c3h 4198 and 4198v cells, but not for the l-m cell, another cell line of c3h origin. the polyoma-specific response in ... | 1975 | 163318 |
| clinical aspects of infection with the epstein-barr virus. | | 1975 | 165289 |
| localization on mitochondrial dna of mutations leading to a loss of rutamycin-sensitive adenosine triphosphatase. | four cytoplasmic mutants of saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive atpase activity but having significant cytochrome oxidase and nadh-cytochrome c reductase have been isolated. genetic studies indicate the mutations to be closely linked to each other and have been assigned to a new locus, pho1. the mutations show a low frequency of recombination with the ol12 locus, suggesting a linkage to this marker. they are not, however, linked to the oli1 locus. linkage o ... | 1976 | 134892 |
| deamination of histamine by peroxidase of neutrophils and eosinophils. | | 1975 | 165295 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| cytokinin ocntents and camp metabolism during growth of escherchia coli. | during escherichia coli growth, we found an inverse correlation between free cytokinin content and camp level. the rates of synthesis of adenylate-cyclase and camp-phosphodiesterase were practically constant. | 1976 | 179840 |
| [changes in the functional state and structure of the adrenals in cc14 poisoning]. | introduction of cci4 in doses of 0.1 and 0.2 ml/100 g to albino rats reduced the steroid production generated by adrenal sections. in 24 and 48 hours after poisoning with a small dose of cci4 (0.1 ml/100 g) there could be seen an increased concentration of 11-oxycorticosteroids in the plasma. morphological signs of a damage suffered by the gland were observed only in poisoning with a large dose of cci4 (0.2 ml/100 g). | 1975 | 179852 |
| microvesicles and vesicles of multivesicular bodies versus "virus-like" particles. | pellets obtained by the centrifugation of unfiltered or filtered fetal bovine serum (fbs) contained, among other things, numerous elongate and spherical microvesicles, ranging from 30 to 60 nm. they possessed a trilaminar (unit membrane) envelope and a moderately electron-dense core. vesicles of multivesicular bodies and microvesicles associated with secretory epithelial cells and human lymphoblastoid cells in suspension culture possessed the same structure and were in the same size range as the ... | 1975 | 165305 |
| [effects of tritoqualine on hepatic and blood levels of histamine after partial hepatectomy in rats]. | looked as a specific inhibitor of histidin-decarboxylase, tritoqualine has been injected subcutaneously to wistar rats immediately after partial hepatectomy and, then, once in 24 hours until 72 hours. it has thus provoked : an unexpected, but transient rise (reaching its apex 3 hours after the operation) of the hepatic and blood histamin concentrations ; then, the expected decrease (more durable and greatest at 48 hours) of the same concentrations. therefore, an effect of tritoqualine on the liv ... | 1977 | 148959 |
| [epidemiological aspects of cytomegalovirus infections]. | | 1975 | 180573 |
| [effect of a chronic iodine deficit in the ration on the development of the infectious process]. | it was revealed that the infectious process in albino rats kept for 4-5 months on an iodine-deficiency diet was characterised by a tendency to dissemination. the seeding efficiency from the parenchymatous organs increased in such animals significantly, whereas the bactericidal properties of the plasma and the phagocytic activity of blood neutrophils decreased; this was apparently associated with depression of the intracellular metabolism reflected in reduction of the cytochromoxidase and peroxid ... | 1975 | 173121 |
| cyclic amp and cyclic gmp: studies utilizing immunohistochemical techniques for the localization of the nucleotides in tissue. | antibodies to the cyclic nucleotides initially were utilized in radioimmunoassays for cyclic amp and cyclic gmp which might be present in mammalian tissues. allowed measurement of the nucleotides on small amounts of tissue in physiologic studies. to gain further insight into the relative roles of cyclic amp and cyclic gmp in cell function, these antibodies have been applied to immunohistochemical studies for the localization of the cyclic nucleotides in tissues and cells. this methodology is use ... | 1975 | 165362 |
| in vivo activation by polymyxin b of phospholipase from pseudomonas aeruginosa and escherichia coli. | | 1976 | 181357 |
| thyroid peroxidase, thyroglobulin, camp and dna in human thyroid. | tissues from normal thyroid, euthyroid multinodular goiter, toxic goiter, hashimoto's thyroiditis, thyroid adenoma, and carcinoma were examined for their content of deoxyribonucleic acid (dna), thyroid peroxidase activity (tpa), thyroglobulin (tg), and adenosine 3',5'-cyclic monophosphate (camp). compared with normal thyroids dna was increased in the neoplastic tissues and decreased in euthyroid multinodular goiter. quantitative changes of thyroglobulin were observed, especially in the thyroid t ... | 1976 | 181398 |
| a simple radioimmunoassay for 3',5', cyclic adenosine monophosphate. | | 1975 | 165745 |
| breakage of yeast: a method for processing multiple samples. | | 1975 | 165747 |
| enzootic with ectromelia symptomatology in sprague-dowley rats. | | 1976 | 181904 |
| effectiveness of orally administered bordetella pertussis vaccine in mice. | oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation. | 1976 | 182602 |
| growth inhibition of proteus mirabilis by cyclic adenosine 3'-5'-monophosphate. | growth of proteus mirabilis on a synthetic agar medium containing either glycerol, galactose, or trehalose as the sole source is inhibited by 5 mm cyclic adenosine 3',5'-monophosphate (camp). inhibition on an agar medium is evident as loss of viability, but in broth camp only slightly inhibits growth rate. inhibition is associated with the accumulation of methylglyoxal in the medium. a nonswarming mutant of p. mirabilis is not inhibited by camp on either of the three carbon sources, but it is se ... | 1976 | 182676 |
| the cornea in connective tissue diseases. | even though lenticular and retinal abnormalities seem to dominate the ophthalmologic picture in marfan's syndrome, the cornea shows significant abnormalities consisting of a striking flattening and corneal astigmatism. the use of conjunctival biopsies followed by histochemical and electron-microscopic evaluation shows low morbidity but an excellent yield of diagnostic information on storage diseases, and an ectopic collagen may be the basis of at least one type of keratoconus. much more work has ... | 1978 | 154085 |
| computerized axial tomography in syringomyelia. | the acta-scanner, a device for computerized axial tomography, permits cross-sectional radiographic study of the entire human body, including the spine. in the acta-scan, the spinal cord appears as a roundish formation surrounded by the less dense subarachnoidal cerebrospinal fluid. the spines of 18 patients with verified (nine cases) or suspected (nine cases) syringomyelia were studied by acta-scanning. in seven of the verified and in four of the nonverified cases, some evidence of cord cavitati ... | 1975 | 162787 |
| biological characters and bacteriophage typing of staphylococcus aureus isolated from chicken staphylococcosis and commercial balanced chicken food in argentine. | during 1976-78, 33 s. aureus strains were isolated from chickens suffering from dermatitis and septicemia, omphalitis, pneumonia, arthritis and tenosynovitis; and from a commercial balanced chicken food. all the strains were classified into the biotype b of hájek and marsálek. twenty-nine (87.8%) strains could be classified into sato et al biological type 4 although they differed from it in its ability to grow at 45 degrees c. the remaining 4 strains differed from the others because they failed ... | 1979 | 162511 |
| muscle and non-muscle cell rna polymerase activity during the development of myocardial hypertrophy. | | 1978 | 151746 |
| cardiovascular adaptations to physical training. | | 1977 | 139843 |
| the role of cyclic adenosine monophosphate in the swarming phenomenon of proteus mirabilis. | the levels of cylic amp and adenyl cyclase in swarming and non-swarming cells of proteus mirabilis and the effect of glucose on swarming have been investigated. the results indicate the camp is required for swarming, but that the flagellar derepression characteristic of swarming does not result from increased camp levels. | 1976 | 185081 |
| selective deficit in feeding behavior following the antero-medial amygdaloid lesions in cats. | | 1975 | 174387 |
| enumeration of antigen reactive t cells against sheep erythrocytes by the virus plaque assay. | | 1976 | 185443 |
| biological values of the infant, juvenile, and adult agouti (dasyprocta sp) with emphasis on microbial flora. | normal values for intestinal flora were determined on four adult, two juvenile, and four infant agoutis (dasyprocta sp) maintained at our institution. serologic, hematologic, biochemical, and histologic observations were also made on these same agoutis, and serologic, microbiologic, and endoparasitic tests were made on serum and fecal samples from agoutis maintained at other institutions. streptococci, lactobacilli and nonenteropathogenic escherichia coli were isolated from cecal contents of all ... | 1976 | 185456 |
| catecholamine secretion by isolated adrenal cells. | isolated adrenal cells were prepared by collagenase digestion of guinea pig adrenal glands. acetylcholine stimulates the secretion of catecholamines by these isolated adrenal cells. acetylcholine-stimulated catecholamine secretion is inhibited by cholinergic blocking agents (atropine and hexamethonium) and by local anaesthetics (tetracaine), and is dependent upon the concentration of ca2+ in the incubation medium. in the presence of ca2+, catecholamine secretion is also stimulated by two divalen ... | 1976 | 174736 |
| opiate receptor: autoradiographic localization in rat brain. | opiate receptor sites in rat brain can be labeled in vivo by [3h]diprenorphine, a potent opiate antagoinst. using techniques to minimize diffusion in fresh, frozen, unfixed brain, we have localized [3h]diprenorphine by autoradiography to visualize the distribution of opiate receptors. silver grains indicative of the binding of labeled [3h]diprenorphine are discretely localized in numerous areas of the brain with very high densities in the locus coeruleus, the substantia gelatinosa of the spinal ... | 1976 | 185626 |
| [immunologic results obtained with an experimental system oftransplantable colonic tumors. possible applications of these results to human intestinal tumors]. | intestinal cancers, morphologically very close to human colo-rectal adeno-carcinoma, have been induced by dimethylhydrazine in inbred rats. graftable lines have been obtained from 5 primary intestinal tumors, and 3 cell lines have been cultivated from the grafts. this model was used to demonstrate carcinofetal antigen(s) on the surface of the intestinal cancer cells. circulating antibodies against tumor-associated antigen(s) have been found in tumor-bearing rats. cancer enhancement was regularly ... | 1975 | 174767 |
| the effects of dbcamp on adreanl chromaffin cells in organotypic culture. | chromaffin cells in organotypic cultures of adrenal glands from 19 day rat embryos developed a marked increase in the volume of their rough endoplasmic reticulum (rer) and enlargement of nucleoli in response to 1 mm dibutyryl cyclic amp (dbcamp) treatment for 17 days in vitro. 0.1 mm dbcamp had a similar but less dramatic effect. when dbcamp was removed from the medium after 11 days in vitro, cells became more like control explants treated with no additives, suggesting the effects of dbcamp were ... | 1976 | 175136 |
| apoproteins of the lipoproteins in a nonrecirculating perfusate of rat liver. | the apoproteins of serum lipoproteins and of lipoproteins present in a nonrecirculating perfusate of rat liver were compared by immunochemical, gel electrophoretic, and solubility techniques. serum and perfusate very low density lipoprotein apoprotein composition were not different. no evidence for the presence of a lipoprotein resembling serum low density lipoprotein was obtained. however, the apoprotein composition of circulatory high density lipoprotein was quantitatively different from the s ... | 1976 | 176290 |
| [effect of vitamins pp and b1 on pentosephosphate pathway enzymatic activity in the kidneys of adrenalectomized rats]. | the activity of glucose-6-phosphate dehydrogenase (ec 1.1.1.44), transketolase (ec 1.1.1.44), transketolase (ec 1.1.1.49), 6-phosphogluconate dehydrogenase (ec 2.2.1.1.) and also the thiaminediphosphate (tdp) content were investigated on the 8th day after adrenalectomy. with compensated sodium dysbalance the removal of suprarenals is shown not to change the activity of dehydrogenases, but it does lower the transketolase activity and the tdp level. a seven-fold administration to adrenalectomized ... | 1978 | 149433 |
| superinfection epithelial nasopharyngeal carcinoma cells with epstein-barr virus. | attempts were made to superinfect epithelial explant cell cultures prepared from nasopharyngeal carcinomas with epstein-barr virus. virus-specific markers were observed in such cultures 3 days after superinfection. in addition, expression of epstein-barr virus early antigens was observed in epithelial cell explant cultures treated with iododeoxyuridine. the results suggest that epithelial cells of at least certain nasopharyngeal carcinomas possess the receptor for the epstein-barr virus and that ... | 1976 | 176664 |
| formation in the dark, of virus-induced deoxyribonuclease activity in anacystis nidulans, an obligate photoautotroph. | in anacystis nidulans, upon infection with cyanophage as-1, after a lag period of 1 h the level of deoxyribonuclease (dnase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. in contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, i.e. as late as 1 h prior to lysis. in complete darkness, the nuclease levels remained unaltered. however, when the infected cells were exposed to light for 1 or 2 h after infection ... | 1976 | 177400 |
| fumarate reduction in proteus mirabilis. | 1. proteus mirabilis formed fumarate reductase under anaerobic growth conditions. the formation of this reductase was repressed under conditions of growth during which electron transport to oxygen or to nitrate is possible. in two of three tested chlorate-resistant mutant strains of the wild type, fumarate reductase appeared to be affected. 2. cytoplasmic membrane suspensions isolated from anaerobically grown p. mirabilis oxidized formate and nadh with oxygen and with fumarate, too. 3. spectral ... | 1976 | 189721 |
| [method for simultaneous study of peristaltic and pendular movements of isolated rabbit ileum]. | a method is described for studying simultaneously the peristalsis and the pendular movements in the isolated pieces of rabbit ileum. at the same time, using this method it is possible to analyse the actions of drugs on the mechanisms which ensure the pendular movements and the propulsive activity, as well as their relationship. the method is relatively simple and only classical and commercially available equipments are needed. with this method the records and the volume of fluid expelled are sui ... | 1978 | 150905 |
| interaction of swine lipoproteins with the low density lipoprotein receptor in human fibroblasts. | hdlc, a cholesterol-rich lipoprotein that accumulates in the plasma of cholesterol-fed swine, was shown to resemble functionally human and swine low density lipoprotein in its ability to bind to the low density lipoprotein receptor in monolayers of cultured human fibroblasts. this binding occurred even though hdlc lacked detectable apoprotein b, which is the major protein of low density lipoprotein. after it was bound to the low density lipoprotein receptor, hdlc, like human and swine low densit ... | 1976 | 177413 |
| comparison of the effects of mecillinam and 6-aminopenicillanic acid on proteus mirabilis, escherichia coli, and staphylococcus aureus. | single strains of proteus mirabilis, escherichia coli, and staphylococcus aureus were grown on filter membranes placed on agar containing concentration series of mecillinam (fl 1060), 6-aminopenicillanic acid (6-apa), or ampicillin. p. mirabilis and e. coli were also exposed to combinations of mecillinam or 6-apa with ampicillin. colony-forming units were counted, and cells were examined by interference phase-contrast and transmission electron microscopy. mecillinam and 6-apa were very effective ... | 1977 | 193439 |
| pivmecillinam in urinary tract infections: a correlation of urinary bactericidal activity with clinical efficacy. | | 1977 | 193813 |
| sequence of the cysteine peptide from the copper-subunit of bovine cardiac cytochrome oxidase. | | 1975 | 169852 |
| patterns of neuromuscular disease. as related to stages of normal embryogenesis in voluntary muscle. | skeletal muscle samples from the upper and lower extremities of 38 human fetuses (6 to 18 weeks' gestation) of both sexes were studied by histologic, histochemical, and electron microscopic methods. ultrastructural morphometry was applied. in the different stages of normal development are found regressive changes, ranging from reversible dystrophic to irreversible necrotic alterations, which are characteristic of either primary myogenic myopathies or primary neurogenic muscle atrophies in older ... | 1979 | 156504 |
| rat adrenocortical dynamics. | 1. the dynamics of the adrenocortical response to adrenocorticotrophic hormone (acth) was studied in anaesthetized, acutely hypophysectomized male rats. 2. acth test-signals were applied either in a jugular vein ('intact infused preparation') or in the aorta through the coeliac artery with the aorta ligated below this artery ('isolated in situ perfused preparation'). the adrenocortical responses were measured directly in samples serially taken from the left adrenal vein. 3. tested acth signals w ... | 1977 | 191596 |
| defects in granulocyte function in various chromosome abnormalities (down's-, edwards'-, cri-du-chat syndrome). | in five infants with autosomal aberrations and diminished resistance to infection (in spite of intact humoral and cellular immune mechanisms) several granulocyte functions (chemotaxis, phagocytosis, intracellular killing and metabolism of killing) were measured. a serum-dependent or a cell-dependent disturbance of phagocytosis of candida albicans was found in two infants with cat-cry syndrome and one with trisomy 18. in one of these children there was an additional serum dependent defect of the ... | 1976 | 176505 |
| assignment of the gene for human uridine monophosphate kinase to chromosome 1 using somatic cell hybrid clone panels. | evidence from mouse-human somatic cell hybrids is presented for the assignment of the gene for uridine monophosphate kinase (atp: nucleoside monophosphate phosphotransferase, e.c. 2.7.4.4) to human chromosome 1. the use of the "clone panel" in this determination and its value as a systematic method for gene mapping is discussed. | 1975 | 172293 |
| pituitary cyclic amp production and mechanism of luteinizing hormone release. | | 1975 | 178535 |
| inhibition of hepatic lipogenesis by adenine nucleotides. | incubation of liver slices and isolated liver cells with adenosine cyclic-3',5'-monophosphate at concentrations which inhibit lipogenesis was found to expand the pool size of the noncyclic adenine nucleotides in the intact cells of the preparations. this observation led to studies which demonstrated that adenosine and adenosine-5'-monophosphate also inhibited lipogenesis and expanded the adenine nucleotide pool size. it is proposed but not proven that the increase in intracellular nucleotides pr ... | 1975 | 172757 |
| the enzymatic reduction of k-vitamins incorporated in the membrane of liposomes. | | 1975 | 178539 |
| subcellular distribution of steroids in rat adrenocortical tissue after incubation in vitro [proceedings]. | | 1977 | 194992 |
| experimental encephalitis caused by herpes simplex virus: comparison of treatment with tilorone hydrochloride and phosphonoacetic acid. | a mouse model of encephalitis caused by herpes simplex virus was used to compare the antiviral activity of tilorone hydrochloride with that of phosphonoacetic acid. these compounds were also administered simultaneously to determine whether the combination had a synergistic effect. rates of survival and concentrations of virus in brain were used as criteria for judging the effectiveness of treatment. the fatal course of encephalitis was not altered by any treatment protocols in which tilorone hyd ... | 1976 | 180204 |
| genetics of resistance of fowl to infection by rna tumour viruses. | | 1976 | 180540 |
| aryl hydrocarbon hydroxylase induction in mammalian liver-derived cell cultures. effects of various metabolic inhibitors on the enzyme activity in hepatoma cells. | | 1976 | 181006 |
| inhibition by rheumatoid factor, anti-fc, and staphylococcal protein a of antibody-dependent cell-mediated cytolysis against herpes simplex virus-infected cells. | incubation of herpes simplex virus-infected human fibroblasts with the serum from a patient with herpes labialis rendered the cells susceptible to immune lysis by human mononuclear leukocytes (mnl) as well as complement. if, before the addition of mnl, the antibody-treated, infected monolayers were incubated with either igm rheumatoid factor (rf), staphylococcal protein a (spa), or anti-fc gamma serum, antibody-dependent cell-mediated cytolysis (adcc) was markedly depressed. spa and anti-fc caus ... | 1976 | 181492 |
| experimental myasthenia due to alpha-bungarotoxin. | neuromuscular transmission was studied electrophysiologically in rabbits intoxicated by alpha-bungarotoxin, a specific inhibitor of acetylcholine receptor. the findings consisted of a slight reduction in amplitude of single evoked muscle action potentials, a decrement in amplitude of successive evoked muscle action potentials post-tetanic potentiation and exhaustion, edrophonium reversal, and no change of muscle action potentials evoked by direct stimulation of the muscle. these were similar to ... | 1976 | 181695 |
| characterization of a stable spheroplast type l-form of proteus mirabilis d 52 as cell envelope mutant. ii. electronmicroscopic investigations. | the ultrastructural analysis of a stable spheroplast type l-form of proteus mirabilis d 52 revealed characteristic alterations in the organization of the cell envelope including defective changes in the cell envelope structure as for instance the loss of a coherent murein layer, the loss of some components in the outer cell wall layer, the formation of small membraneous vesicles at the tips of loose extensions of the cell wall, a decrease in associations and bindings between wall and membrane, a ... | 1976 | 184605 |
| virus-induced murine leukemia involving two subpopulations of lymphocytes. | | 1975 | 183714 |
| phosphorylated sites of calf thymus h2b histone by adenosine 3':5'-monophosphate-dependent protein kinase from bovine cerebellum. | | 1976 | 183776 |
| annealing and hybridization properties of herpes simplex virus type 1 dna. | the hybridization properties of the herpes simplex virus type 1 (hsv) genome have been analysed. the dna has a kinetic complexity of 1 x 10(-8). e. cole rna polymerase was found to initiate synthesis at about 70 sites on the hsv dna. the in vitro rna product from this reaction was complementary to about 80% of the hsv genome. the rna-dna hybridization rate constant (kh) was determined using conditions of both rna excess and dna excess. using this rate constant one can analyse the content of hsv ... | 1976 | 184238 |
| normal rabbit alveolar macrophages. ii. their primary and secondary lysosomes as revealed by electron microscopy and cytochemistry. | in this investigation, vacuoles containing tubular myelin proved to be digestive compartments with cytochemical reactivity for acid phosphatase and arylsulfatase. these cytochemical markers identify the secondary lysosomes, known to contain enzymes capable of hydrolyzing phospholipids like surfactant. therefore, it appears that alveolar macrophages possess the enzymatic machinery for the degradation of the tubular myelin found in their digestive vacuoles. although it thus appears evident that al ... | 1976 | 185318 |
| hypertensive effect of 17alpha, 20alpha-dihydroxyprogesterone and 17alpha-hydroxyprogesterone in the sheep. | | 1976 | 185522 |
| factors influencing the apparent molecular weight of collagenase produced by human-skin explants. | | 1977 | 195813 |
| elimination of proteus mirabilis 51cr endotoxin from the liver in rats. | using isotope methods, elimination of the endotoxin of proteus mirabilis labeled with chromium (crepm) from the liver of rats was studied. the following studies were carried out: intravital exploration of the liver with a scintillation probe, measurements of radioactivity of organs and excreted urine and stools, scrintigraphy of the liver, binding of crepm by subcellular fractions of hepatocytes, and the influence of selected drugs (polymyxin and hydrocortisone) on elimination of crepm from the ... | 1977 | 195551 |
| synthesis and evaluation of (des-asp1)angiotensin i as a precursor for (des-asp1)angiotensin ii ("angiotensin iii"). | the nonapeptide [des-asp1]angiotensin i (iv), synthesized by merrifield's solid-phase procedure, was tested as a possible substrate for the converting enzymes from porcine lung and plasma. iv, [des-asp1]angiotensin ii (iii), [des-(asp1,arg2)]angiotensin ii (v), [des-(asp1,arg2,val3)]angiotensin ii (vi), [sar1,ile8]angiotensin ii (vii), and [des-asp1,ile8]angiotensin ii (viii) possessed 0.5, 20, 2, 0 less than 0.1, and less than 0.01% of the inotropic activity (rabbit atria), 1, 15, 5, 0, 3, and ... | 1975 | 172631 |