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ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate.new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ...201627667823
enterobacterial colonization in captive red-eared sliders (trachemys scripta elegans).the handling of turtles and other reptiles can be associated with risk of pathogenic enterobacteria transmission, mainly salmonella spp. the aim of this study was to identify the enterobacteria in cloacal swabs of 39 red-eared sliders (trachemys scripta elegans). cloacal swabs from 39 captive individuals were analyzed. after sample enrichment in brain-heart infusion broth and 1% peptone water, bacterial isolation was performed through cultivation in blood, macconkey and xylose lysine desoxychola ...201425632683
a rare case of raoultella planticola urinary tract infection in an immunocompromised patient with multiple myeloma.raoultella planticola is a gram-negative rod associated with soil and aquatic environments that has rarely been associated with human infections.201728271042
unexpectedly high occurrence of escherichia coli and staphylococcus aureus isolates from raw milk in ilam, western iran.raw milk contains diverse nutritional components that provide a suitable medium for spoilage and the growth of potentially pathogenic microorganisms. unpasteurized milk consumption by a large number of people can threaten health and increase public concerns. in this study, sixty-two raw cow’s milk samples were collected from the dairy farms of ilam, western iran. all samples were collected in sterilized containers and were transferred via ice boxes to the laboratory. isolates were then identifie ...201626753648
genetic, biochemical characterization and mutagenesis of the chromosomal class a β-lactamase of raoultella (formerly klebsiella) terrigena.chromosomal class a β-lactamases have been characterized in raoultella ornithinolytica and raoultella planticola. the purpose of this study was to characterize that of raoultella terrigena.201526092758
a comparative study on phosphotransferase activity of acid phosphatases from raoultella planticola and enterobacter aerogenes on nucleosides, sugars, and related compounds.natural and modified nucleoside-5'-monophosphates and their precursors are valuable compounds widely used in biochemical studies. bacterial nonspecific acid phosphatases (nsaps) are a group of enzymes involved in the hydrolysis of phosphoester bonds, and some of them exhibit phosphotransferase activity. nsap containing enterobacter aerogenes and raoultella planticola whole cells were evaluated in the phosphorylation of a wide range of nucleosides and nucleoside precursors using pyrophosphate as ...201423995227
carbapenemase-producing enterobacteriaceae in finland: the first years (2008-11).carbapenemase-producing enterobacteriaceae (cpe) are becoming a global problem; they are often resistant to nearly all available antibiotics. here we report details on all finnish cpe isolates found until the end of 2011: carbapenemase genes, travel history and multilocus sequence typing (mlst) data.201222855858
raoultella planticola bacteremia following consumption of seafood.raoultella planticola is a gram-negative bacillus commonly found in water, soil and aquatic environments. there have only been 16 cases of r planticola infection documented in the literature to date. r planticola possesses the ability to convert histidine to histamine and can produce symptoms of scombroid poisoning when poorly prepared seafood is consumed in large amounts. the present report describes a case involving a 56-year-old woman who presented with r planticola bacteremia and symptoms co ...201425285133
low virulence? clinical characteristics of raoultella planticola bacteremia.numerous case reports regarding raoultella planticola infection have accumulated in the literature; however, its significance as a clinical pathogen remains unknown. we performed a retrospective review of r. planticola bacteremia to characterize its clinical features, antimicrobial susceptibility, and patient outcome.201425047268
[cholangitis and bacteraemia caused by raoultella planticola]. 201525023852
heat resistance of histamine-producing bacteria in irradiated tuna loins.consumption of foods high in biogenic amines leads to an illness known as histamine, or scombrotoxin, poisoning. the illness is commonly associated with consumption of fish with high levels of histamine ( $ 500 ppm). the objective of this study was to determine and compare the heat resistance of five histamine-producing bacteria in irradiated albacore tuna loins. heat-resistance parameters (d- and z-values) were determined for morganella morganii, raoultella planticola, hafnia alvei, and enterob ...201323992506
bioprospecting of lipolytic microorganisms obtained from industrial effluents.the lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. the aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in pelotas, rs/brazil. bacteria were identified using gram stain and biochemical tests (vitek 2(r)). fungi were identified according to macro and micromorphology characteristics. the extracellular lipase production was evaluated using the rhoda ...201627556331
a novel case of chronic conjunctivitis in a 58-year-old woman caused by raoultella.a 58-year-old woman presented to eye emergency with a chronic conjunctivitis which was diagnosed by laboratory microbiological testing to be due to the environmental pathogen raoultella planticola. the organism was sensitive to chloramphenicol and the patient made a rapid recovery on these drops. this is the first report of this organism infecting the eye.201424865691
acute prostatitis caused by raoultella planticola in a renal transplant recipient: a novel case.we present a unique case of acute bacterial prostatitis caused by a very rare human pathogen, raoultella planticola, in a renal allograft recipient 3.5 months post transplantation. only a few cases of human infection by this pathogen have been reported worldwide. the present study reports the case of a 67-year-old man who was admitted to our transplant unit 3.5 months post transplantation with fever, dysuria, suprapubic pain, symptoms and signs of acute prostatitis, and elevated markers of infla ...201424750300
raoultella planticola-associated cholangitis and sepsis: a case report and literature review. 201424733944
multidrug and heavy metal-resistant raoultella planticola isolated from surface water.a surface water isolate of raoultella sp. having both multidrug- and multimetal-resistant ability was isolated and identified as raoultella planticola. r. planticola displayed resistance to 15 drugs like ampicillin, amoxicillin/clavulanic acid, aztreonam, erythromycin, imipenem, oxacillin, pefloxacin, penicillin, piperacillin, piperacillin/tazobactam, rifampin, sulbactam/cefoperazone, ticarsillin, ticarsillin/clavulanic acid, vancomycin, and to 11 heavy metals like aluminum, barium, copper, iron ...201323754693
raoultella planticola bacteraemia secondary to gastroenteritis.we present the first case of raoultella planticola bacteria in human infections with a direct relationship between fish intake and enteric infection. the patient was treated with antibiotherapy (cefotaxime). it is important to consider this organism in the differential diagnosis of enteric fever and even more with previous ingestion of fish.201323574552
raoultella planticola bacteraemia. 201222820690
characterization of rhizobacterial strain rs-2 with acc deaminase activity and its performance in promoting cotton growth under salinity stress.a plant growth-promoting rhizobacterial strain rs-2 with 1-aminocyclopropane-1-carboxylate (acc) deaminase activity was isolated from salinized soils using acc as the sole nitrogen source. based on its physiological and biochemical properties and 16s rdna sequence analysis, this strain was identified as raoultella planticola. the maximum value of nitrogen fixation, dissolved phosphorus and dissolved potassium of rs-2 were 148.8 μg/ml, 205.0 and 4.31 mg/l, respectively within 192 h liquid culture ...201222806112
a novel case of raoultella planticola urinary tract infection.raoultella species are gram-negative, non-motile bacilli primarily considered to be environmental bacteria (bagley et al.; curr microbiol 6:105-109, 1981). r. planticola has rarely been documented as a cause of human infections and has never been reported to cause urinary tract infections. we report the first case of r. planticola cystitis.201322802099
prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species.recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ...201526197285
identification of raoultella terrigena as a rare causative agent of subungual abscess based on 16s rrna and housekeeping gene sequencing.a 63-year-old-man was admitted to our hospital with severe subungual abscess. bacteria were isolated from pus samples, and an inconsistent identification was shown by vitek 2 system and maldi-tof mass spectrometry as raoultella planticola and raoultella terrigena, respectively. molecular identification by 16s rrna sequencing suggested that the isolate is r. terrigena, and this was further demonstrated by sequencing three housekeeping genes (rpob, gyra, and parc) with phylogenetic analysis. to ou ...201627379169
a single-center, six-year evaluation of the role of pulsed-field gel electrophoresis in suspected burn center outbreaks.pulsed-field gel electrophoresis (pfge) has been used as an adjunct to traditional hospital epidemiology in numerous outbreak investigations, including in burn centers. however, its most effective real-world application remains unclear, with few longitudinal descriptions of use.201627180602
bacteremia with raoultella planticola in the setting of acute pancreatitis complicated with acute cholangitis.the bacterium raoultella planticola (r planticola) is a rare pathogen in humans. we report a case of mild acute pancreatitis (map) of biliary origin with cholangitis and bacteremia with r planticola in association with pancreatic panniculitis (pp). a 55-year-old woman with rheumatic multiple valvulopathy presented with epigastric pain radiating to the back in a belt-like fashion for 48 hours, jaundice, choluria, acholia, and fever (38.2oc). lab tests showed: amylase 2126 u/l, alt 124 u/l, ast 16 ...201728229615
a case of urinary tract infection caused by raoultella planticola after a urodynamic study.here we report the case of a patient who developed urinary tract infection after a urodynamic study. the causative agent was raoultella planticola, a rare opportunistic pathogen that usually invades immunocompromised patients. while a urinary tract infection with r. planticola has been previously described, this is the first report in which an r. planticola infection developed after a urodynamic study. we postulate that the mechanism of infection was direct invasion of the urinary tract from con ...201727883875
evaluation of enrichment protocols for bacterial endosymbionts of ciliates by real-time pcr.large-scale studies on obligate bacterial endosymbionts may frequently require preliminary purification and enrichment protocols, which are often elaborate to set up and to evaluate, especially if the host organism is a protist. the purpose of this study was to develop a real-time pcr-based strategy and employ it for assessing two of such enrichment protocols for holospora caryophila, hosted by the ciliate paramecium. four ssu rrna gene-targeted real-time pcr assays were designed, which allowed ...201626894821
a case of pneumonia caused by raoultella planticola.raoultella species are gram-negative, non-motile, aerobic bacilli that are primarily considered as environmental bacteria. raoultella planticola is reportedly a rare cause of human infections. also, the definite pathological mechanism of raoultella planticola is currently unknown. we report a case of pneumonia caused by raoultella planticola.201626770234
four cases of raoultella planticola conjunctivitis.this brief report of four cases of conjunctivitis caused by raoultella planticola provides a description of possibly the first documented cases of this eye infection.201626742869
acute raoultella planticola cystitis in a child with rhabdomyosarcoma of the bladder neck.raoultella planticola is a gram-negative, non-motile, aerobic bacillus. it is an environmental bacteria found in soil and water, and a very rare cause of local or systemic infection in humans. although some adult cases of r. planticola infection have been reported, childhood local or systemic infection caused by r. planticola is very rare. reported herein is a rare case of acute cystitis due to r. planticola in a 16-month-old boy with rhabdomyosarcoma of the bladder neck, and a review of the lit ...201526508180
a versatile chitosan/zno nanocomposite with enhanced antimicrobial properties.porous chitosan membrane was fabricated by casting method using silica particles. simultaneously nano zno was synthesized by green-synthesis method using tung ting oolong tea extract. chitosan membrane was combined with nano zno in order to increase its antimicrobial activity. through observations obtained from various techniques such as xrd, sem, ft-ir, uv-visible and fluorescence emission analyses, chitosan was seen to be able to incorporate nano zno in the nanocomposite membrane. a blue shift ...201526111911
draft genome sequence of raoultella terrigena r1gly, a diazotrophic endophyte.raoultella terrigena r1gly is a diazotrophic endophyte isolated from surface-sterilized roots of nicotiana tabacum. the whole-genome sequence was obtained to investigate the endophytic characteristics of this organism at the genetic level, as well as to compare this strain with its close relatives. to our knowledge, this is the first genome obtained from the raoultella terrigena species and only the third genome from the raoultella genus, after raoultella ornitholytic and raoultella planticola. ...201526067957
raoultella planticola peritonitis in a patient on continuous ambulatory peritoneal dialysis.a 65-year-old man on continuous ambulatory peritoneal dialysis was admitted with peritonitis. empirical antibiotic therapy was initiated, and raoultella planticola was identified in the peritoneal fluid culture. we treated the patient with intraperitoneally administered ciprofloxacin and ceftazidime according to the antibiotic susceptibility. his condition improved, and he was well treated with a 2-week antibiotic course.201525958102
a novel case of raoultella planticola urinary tract infection in a female: comment on 'nosocomial pneumonia caused by carbapenem-resistant raoultella planticola: a case report and literature review'. 201525944570
[auxin production by klebsiella planticola strain tskha-91 and its effect of development of cucumber (cucumis sativus l.) seeds].capacity of klebsiella planticola strain tsjha-91 for synthesis of indolyl-3-acetic acid (iaa) and other auxins was studied. the qualitative and quantitative composition of these compounds depends on the presence of exogeneous tryptophan and on the nitrogen source. the highest iaa yield was obtained at the stationary phase of growth. addition of l-tryptophan to the medium resulted in a significant increase (up to 85.5 microg/ml) of auxin biosynthesis, especially in the presence of nitrates. thin ...201525844466
isolation of raoultella planticola from refillable antimicrobial liquid soap dispensers in a dental setting.liquid antimicrobial soaps are commonly used in the dental health care setting for hand washing to minimize the potential spread of infectious agents to health care workers and patients. the purpose of the current study was to evaluate possible bacterial contamination of antimicrobial liquid soap dispensers located in 2 institutional comprehensive dental care clinics.201525819655
characterization of raoultella planticola rs-2 microcapsule prepared with a blend of alginate and starch and its release behavior.to judiciously use raoultella planticola rs-2 and develop its biodegradable and controlled-release formulations, rs-2 was encapsulated with various combinations of sodium alginate (naalg) and starch. sodium alginate, soluble starch, and cacl2 showed good biocompatibility with rs-2 for preparing microcapsules. these microcapsules were spherical in shape and their particle size, embedding rate, swelling ratio of rs-2 microcapsules and release numbers of viable rs-2 cells increased with the increas ...201424906754
emerging pathogen: a case and review of raoultella planticola.raoultella planticola has been considered a relatively harmless gram-negative bacteria, rarely associated with clinical infection. however, in recent years, the frequency at which severe infection by r. planticola and drug-resistant strains are reported in literature has increased. here, we present one case of acute cholecystitis caused by r. planticola, and review all previously reported cases of the infection in an attempt to identify new trends in biological and clinical features of r. planti ...201424902523
a rare case of cholecystitis caused by raoultella planticola.a 62-year-old female presented with right upper quadrant pain. clinical examination and ultrasound scan were consistent with gallstones and acute cholecystitis. she received 3 days of intravenous co-amoxiclav and was discharged with 5-days of oral antibiotics with arrangements to return for an elective cholecystectomy. this was performed 5 months later which revealed an inflamed gallbladder and a localised abscess secondary to gallbladder perforation. fluid from the gallbladder was taken which c ...201222690225
isolation and lipid degradation profile of raoultella planticola strain 232-2 capable of efficiently catabolizing edible oils under acidic conditions.the lipids (fats and oils) degradation capabilities of soil microorganisms were investigated for possible application in treatment of lipids-contaminated wastewater. we isolated a strain of the bacterium raoultella planticola strain 232-2 that is capable of efficiently catabolizing lipids under acidic conditions such as in grease traps in restaurants and food processing plants. the strain 232-2 efficiently catabolized a mixture (mixed lipids) of commercial vegetable oil, lard, and beef tallow (1 ...201322395910
synthesis and in vitro antioxidant and antimicrobial studies of novel structured phosphatidylcholines with phenolic acids.novel phenoylated phosphatidylcholines were synthesized from 1,2-dipalmitoyl phosphatidylcholine/egg 1,2-diacyl phosphatidylcholine and phenolic acids such as ferulic, sinapic, vanillic and syringic acids. the structures of phenoylated phosphatidylcholines were confirmed by spectral analysis. 2-acyl-1-lyso phosphatidylcholine was synthesized from phosphatidylcholine via regioselective enzymatic hydrolysis and was reacted with hydroxyl protected phenolic acids to produce corresponding phenoylated ...201727979256
multidrug resistance found in extended-spectrum beta-lactamase-producing enterobacteriaceae from rural water reservoirs in guantao, china.extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae have been isolated from humans and animals across the world. however, data on prevalence of esbl-producing enterobacteriaceae from rural water reservoirs is limited. this study aimed to isolate and characterize esbl-producing enterobacteriaceae in rural water reservoirs in guantao, china. esbl-producing enterobacteriaceae were found in 5 (16.7%) of 30 sampled rural water reservoirs. sixty-six individual isolates expressing an e ...201525873918
synthesis, characterization, and antibacterial and anticancer screening of {m(2+)-co (3+)-m (2+)} and {co (3+)-m (2+)} (m is zn, cd, hg) heterometallic complexes.the cobalt(iii) complexes et(4)n[co(l(1))(2)] and [co(l(2))(3)] [h(2)l(1) is 2,6-bis(n-(2-pyridyl)carbamoyl)pyridine and hl(2) is 2-(n-(2-pyridyl)carbamoyl)pyridine] were used as the building blocks for preparing a series of {m(2+)-co(3+)-m(2+)} (where m is zn, cd, or hg) and {co(3+)-m(2+)} (where m is zn or cd) heterometallic complexes. all heterometallic complexes were characterized using a host of spectroscopic methods (ir, nmr, and uv/vis spectroscopy and mass spectrometry), elemental analys ...201223001051
ndm-1-producing enterobacteriaceae in a teaching hospital in shanghai, china: incx3-type plasmids may contribute to the dissemination of blandm-1.to provide the epidemiological dissemination and the genetic characteristics of bla(ndm-1) in a teaching hospital in shanghai, china.201525743762
nosocomial pneumonia caused by carbapenem-resistant raoultella planticola: a case report and literature review.raoultella planticola is a rare opportunistic pathogen usually invaded immunocompromised patients and sometimes even causes fatal infections. recently, there is growing concern about the emergence of carbapenem resistance in this species. here, we describe one case of hospital-acquired pneumonia due to a carbapenem-resistant r. planticola (crrp) co-producing klebsiella pneumoniae carbapenemase and extended-spectrum β-lactamase. a literature review was performed to indicate the microbiological an ...201525595510
contamination of liquid soap for hospital use with raoultella planticola.this article reports the contamination of a batch of liquid soap for hospital use with raoultella planticola. the micro-organism was first identified as klebsiella pneumoniae due to the inability of automated systems to characterize this species. there is a need to strengthen the inspection of cosmetic products to be used in the hospital setting. it is recommended that hospitalized patients at the highest risk of infection should use antimicrobial soaps for personal hygiene. the incidence of inf ...201424559578
regioselective synthesis, antimicrobial evaluation and theoretical studies of 2-styryl quinolines.2-styryl quinolines (9a-l) have been synthesized regioselectively from 2-methyl-quinoline by using naoac in water acetic acid binary solvents and evaluated for their antibacterial activity against both gram-positive and gram-negative strains. among these, the compounds 12 and 8 were found to be active against both bacterial strains. compounds 9b, 9f, 9g, 9i, 9j and 9k were the most active among the series exhibiting mic values ranging between 1.9 and 31.2 μg ml(-1) against different bacterial st ...201525465871
a new emerging oral infection: raoultella planticola in a boy with haematological malignancy.oral mucositis is a common complication in pediatric cancer patients, affecting up to 80% of children. due to neutropenia and disruption of the mucosal barrier, chemotherapy-induced oral mucositis is often complicated by super-infections.201728349509
a cardiac implantable device infection by raoultella planticola in an immunocompromized patient.introduction. infection of cardiac implantable electronic devices is a severe condition associated with high mortality, particularly in patients who are dependent upon heart-pacing devices. staphylococci are found in 70 % of reported cases. case presentation. we report the case of a cardiac-pacemaker infection in a 79-year-old man, cumulating a history of rheumatoid arthritis treated by corticosteroids and methotrexate by a recently identified micro-organism: raoultella planticola. he presented ...201728348805
joint infection due to raoultella planticola: first report.the genus raoultella has been separated from the genus klebsiella in 2001. two main species are responsible for human infections: r. ornithinolytica and r. planticola. the most frequent infections due to r. planticola include cystitis, pneumonia and bacteremia (mostly in immunocompromised hosts). to date, no joint or bone infection has been reported. we describe the first case of septic arthritis due to r. planticola following an arthroscopy with intra-articular injection of corticosteroids. evo ...201728337666
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