| reduction of fensulfothion to fensulfothion sulfide by klebsiella pneumoniae. | a cell suspension of klebsiella pneumoniae converted the organophosphorus pesticide fensulfothion to a product that was shown by chemical oxidation, gas-liquid chromatography, infrared spectrophotometry, and mass spectrometry to be fensulfothion sulfide. further alteration of this metabolite was not noted. | 1977 | 143907 |
| in vitro activity of spectinomycin against recent urinary tract isolates. | the susceptibilities to spectinomycin of 303 recent urinary tract isolates were determined and compared to the susceptibilities of those strains to ampicillin, tetracycline, and gentamicin. based on minimal inhibitory concentrations, 84% of escherichia coli, klebsiella, and enterobacter, 31% of other enterobacteriaceae, 7% of staphylococcus aureus and streptococcus (including enterococci), and 0% of pseudomonas aeruginosa were susceptible to concentrations of spectinomycin that are easily surpas ... | 1977 | 144457 |
| [simplified diagnostic scheme for enterobacteriaceae]. | after testing 18 experimental variants against 40 reference strains belonging to different enterovacteriaceae groups, the authors chose a multitest medium that permits concomitant testing of mobility, indol, lysinedecarboxylase and phenylalanine deaminase (milp). in this medium, the reactions of 3734 strains belonging to the genera escherichia, shigella. salmonella, citrobacter, klebsiella, enterobacter, serratia. proteus and providencia were concordant with the reactions in individual test medi ... | 1977 | 144994 |
| [the effect of a mixture of bacterial ribosomes and membrane fractions of klebsiella pneumoniae on the development of a transplanted epithelial cancer in rats]. | an association of bacterial ribosomes and cell-wall of klebsiella pneumoniae was shown to reduce the incidence of an epidermoid carcinoma in sprague-dawley rats when administered subcutaneously prior the tumoral graft. mononuclear cells accumulate around the tumor. this association was considered as a non specific adjuvant stimulating the host's intrinsic immunity to tumor antigens. | 1977 | 145286 |
| molybdenum cofactors from molybdoenzymes and in vitro reconstitution of nitrogenase and nitrate reductase. | a molybdenum cofactor (mo-co) from xanthine oxidase (xanthine:oxygen oxidoreductase, ec 1.2.3.2) can be isolated from the enzyme by a technique that has been used to isolate an iron-molybdenum cofactor (femo-co) from component i of nitrogenase. n-methylformamide is used for the extraction of these molybdenum cofactors. mo-co from xanthine oxidase activates nitrate reductase (nadph:nitrate oxidoreductase, ec 1.6.6.2) in an extract from neurospora crassa mutant strain nit-1; however, femo-co is un ... | 1977 | 146198 |
| [concentration of antibiotics in long term embolized kidneys (author's transl)]. | in 7 dogs renal arterial embolization was performed using seldinger technique and inflatable swan ganz balloon catheter by injection of methyl methacrylate/butyl 2 cyano acrylat. six months later tissue concentration of cefazolin was measured in the embolized and kontralateral kidneys and in serum two hours after a short infusion of 1 g of cefazolin. the concentrations in serum and homogenized kidney tissue were determined by means of the agar well diffusion method. angiography studies showed no ... | 1978 | 147821 |
| [emphysematous pyelonephritis]. | | 1978 | 148420 |
| [enterotoxins of gramnegative intestinal bacteria (author's transl)]. | | 1978 | 152163 |
| [laryngeal subglottic localization of rhinoscleroma]. | | 1979 | 156777 |
| current concepts in connective tissue disorders. | | 1979 | 157790 |
| pseudomonas aeruginosa gram-negative folliculitis. | three patients with sudden, unmanageable exacerbation of acne vulgaris were shown to have gram-negative folliculitis due to pseudomonas aeruginosa. in each patient, the source of the pseudomonas proved to be an otitis externa infection. in contrast to previous cases of gram-negative folliculitis due to proteus, escherichia coli, or klebsiella, the anterior nares were not colonized. treatment of the otitis externa and the gram-negative folliculitis with acetic acid compresses and topical antibiot ... | 1979 | 159666 |
| siderophore synthesis in klebsiella pneumoniae and shigella sonnei during iron deficiency. | klebsiella pneumoniae 298/53 and shigella sonnei 43-gg9 exhibited restricted growth and enterochelin synthesis only under iron-deficient conditions. s. sonnei also produced an unidentified secondary hydroxamate siderophore. | 1979 | 160409 |
| [scleroma]. | | 1979 | 162348 |
| experimental enzyme evolution. | | 1979 | 162365 |
| the nitrogen-fixing complex of bacteria. | | 1975 | 164247 |
| [comparison of in vitro activity of ciclacillin and other penicilline(author's transl)]. | | 1975 | 164612 |
| prophylactic antibiotics in cesarean section. | using a random selection procedure, 100 patients were evaluated in order to determine the value of prophylactic antibiotics in cesarean section. all patients were evaluated for febrile morbidity, and specific infection sites were recorded. significant differences were found between the treated and control groups and between cesarean sections performed prior to the onset of labor and after the onset of labor. | 1975 | 164642 |
| chemotherapeutic nitroheterocycles. 18. 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups. | 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups and related compounds (41-69, table ii) were synthesized and their antimicrobial activities were evaluated (table iii). some of these compounds (e.g. 47, 52, and 59) suprisingly exhibited a broad antibacterial spectrum including proteus species and pseudomonas aeruginosa. extraordinary antitrichomonal activities could also be observed in vitro (mic of compound 59, 0.0004 pg/ml) and ... | 1975 | 166185 |
| [experimental investigations concerning spectrum, serum concentration, and excretion of spectacillin (author's transl)]. | by means of the diffusion test, the range of action of the ampicillin successive preparation spectacillin was determined using 12 000 pathological germs freshly isolated from in-patients. the result was a range of action similar to that of ampicillin. a comparison, however, is only possible in case the test platelets (without over-dosage) are each charged with exactly, e.g., 25 gamma. the analyses of serum concentrations have shown that spectacillin is satisfactorily absorbed after oral as well ... | 1975 | 166292 |
| structural studies of the capsular polysaccharide of klebsiella type 81. | the structure of the capsular polysaccharide from klebsiella type 81 has beeninvestigated. methlylation analysis, uronic acid degradation, smith degradation, andgraded acid hydrolysis were the principle methods used. theanomeric nature of theglycosidic linkages was determined by characterization of fragments obtained from thevarious methods of degradation used. one of the l-rhamnosidic linkages was notpresent in any of these fragments, but is assumed to be an alpha-linkage from consideerationsof ... | 1975 | 166754 |
| structural studies of the capsular polysaccharide of klebsiella type 59. | the structure of the capsular polysaccharide from klebsiella type 59 has been investigated by methylation analysis, a modified smith-degradation procedure, and uronic acid degradation followed by oxidation and elimination of the substituents of the oxidized residue. the oligomeric fragments produced by these degradative procedures were isolated and characterized. o-acetyl groups were identified and their position determined. the polysaccharide consists of the following pentasaccharide repeating- ... | 1975 | 166755 |
| structural studies of the capsular polysaccharide of klebsiella type 28. | the capsular polysaccharide from klebsiella type 28 has been studied by methylation analysis, a modified smith-degradation procedure, and uronic acid degradation with subsequent oxidation and elimination of the substituents of the oxidized residue. the polysaccharide contained the hexasaccharide repeating-unit shown below. the terminal d-glucopyranose residue was hydrolysed by emulsin, indicating a beta linkage. the anomeric natures of other glycosidic linkages were determined by characterizatio ... | 1975 | 166756 |
| interferon production in mice by the capsular polysaccharide of klebsiella pneumoniae. | the capsular polysaccharide of klebsiella pneumoniae (cps-k) type 1, kasuya strain, induces interferon production in the blood of mice when injected intravenously. cps-k resembles bacterial endotoxin (lipopolysaccharide) in the time pattern of interferon production, with peak levels 2h after injection. cps-k on a weight basis exhibits a more potent interferon-inducing effect than lipopolysaccharide. the active substance responsible for the interferon-inducing activity of cps-k is the neutral cps ... | 1975 | 166921 |
| antibacterial activity of tobramycin against gram-negative bacteria and the combination of ampicillin/tobramycin against e. coli. | the antibacterial activity of tobramycin, gentamicin, erythromycin, cloxacillin, kanamycin, cephalexin, penicillin, carbenicillin and polymyxin were compared against 303 clinical bacterial isolates from a pediatric hospital patient population. standard disk diffusion and agar-dilution methods were employed. significant activity was demonstrated for tobramycin against pseudomonas, klebsiella, escherichia coli and both staphylococcus aureus and albus; tobramycin was significantly more active again ... | 1975 | 168040 |
| endotracheal antibiotics for the prevention of tracheobronchial infections in tracheotomized unconscious patients. a comparative study of gentamicin and aminosidin-polymyxin b combination. | endotracheal administration of gentamicin has been compared to the endotracheal administration of aminosidin plus polymyxin b as a preventive measure against tracheobronchial infections in 25 and 22 tracheotomized patients respectively who had been admitted to a neurosurgical unit. both series were comparable as far as underlying disease, duration of hospitalization, surgical therapy. both regimens were similarly effective from the bacteriologic and clinical points of view. both regimens were si ... | 1975 | 169104 |
| effect of klebsiella pneumoniae enterotoxin on intestinal transport in the rat. | the effects on intestinal transport of either a semipurified preparation of enterotoxin elaborated by klebsiella pneumoniae or similaryly prepared control material were tested by marker perfusion studies in the small intestine of rats. at a concentration of 2 mg/ml, the enterotoxin produced net secretion of water, na, and cl in both jejunal and ileal segments; hco3 transport was not affected. net secretion was evident within 30 min after intorduction of the toxin and was maximal after 90 min. th ... | 1975 | 169297 |
| antimicrobial susceptibility of clinical isolates of gram-negative bacilli. | no one can doubt the increased incidence of gram-negative bacillary infections and the importance of an awareness of this increase. today's hospital, where one finds aggressive surgery, patients with multiple indwelling polyethylene lines and foley catheters, and widespread use of prophylactic antibiotics, serves as a haven for resistant gram-negative bacilli. twenty-five strains of escherichia coli, klebsiella sp, enterobacter sp, pseudomonas sp, indole-negative proteus sp, indole-positive prot ... | 1975 | 169593 |
| independent constitutive expression of the aerobic and anaerobic pathways of glycerol catabolism in klebsiella aerogenes. | klebsiella aerogenes dissimilates glycerol aerobically via an inducible pathway initiated by an adenosine triphosphate-linked kinase that converts the substrate to sn-glycerol 3-phosphate. phosphorylated glycerol is then dehydrogenated to dihydroxyacetone phosphate by an enzyme characteristic of a flavoprotein. anaerobically, the organism dissimilates glycerol via an inducible pathway initiated by a nicotinamide adenine dinucleotide-linked dehydrogenase that converts the substrate to dihydroxyac ... | 1975 | 170247 |
| characterization of the respiratory nitrate reductase of klebsiella aerogenes as a molybdenum-containing iron-sulfur enzyme. | 1. in respiratory nitrate reductase i of klebsiella aerogenes, 0.24 atom of molybdenum, eight iron-sulfur groups and four tightly bound, non-heme iron atoms per molecule of enzyme (mr 260 000) are found. 2. epr spectra at 83 degrees k of oxidized and reduced nitrate reductase i show complex lines at g = 2.02 and g = 1.98, which are more intense in the reduced than in the oxidized enzyme. the resonances, the shape and intensity of which are rather temperature insensitive, are attributed to two sp ... | 1975 | 170983 |
| [electron microscopical investigation of rhinoscleroma (author's transl)]. | a boy from togo with a resistent rhinoscleroma was observed. the specific granuloma was examinated by histological and electron microscopic methods. the plasma cells form fibrillary material or guttate russel bodies in the rough endoplasmatic reticulum. the morphology of mikulicz-cells and klebsiella rhinoscleromatis is described. the cells are supposed to be (histiocytic) macrophages. the surface of the germs is coated with a fimbria-like radiate substance which in the vacuoles of the cells is ... | 1975 | 174498 |
| [bactericidal dosie-activity relationships with e. coli, k. pneumoniae and staph. aureus (author's transl)]. | in investigations into the dosis-activity relationships of bactericidal antibiotics used against e. coli, k. pneumoniae and staph. aureus four types of activity could be demonstrated. the penicillin type shows almost no improvement in bactericidal activity despite increasing the dosage above a certain level. an increase in concentration of aminoglycoside antibiotics led to a more rapid killing of the bacteria. in cefalotin there was a linear dosis-activity relationship: rising concentrations of ... | 1976 | 174881 |
| antibacterial activity of oxidized regenerated cellulose. | it has been demonstrated that oxidized regenerated cellulose promptly and markedly reduces the bacterial census of ten different strains of common pathogens when exposed to them in vitro. this was not true of two other hemostatic agents tested, namely, absorbable gelatin sponge and topical thrombin. wounds in guinea pigs in which oxidized regenerated cellulose was placed and infected with one of three pathogens had healing per primum in 39 of 40 instances. in similar control wounds and incisions ... | 1976 | 176740 |
| [clinical use of sodium hypochlorite for local treatment and prevention of urinary tract infections and therapy of contracted bladders (author's transl)]. | a stable commercial electrolytic sodium hypochlorite solution was tested in 63 urologic patients. the complete germicidal action against all sorts of bacteria was shown in suspension-tests. irrigation studies of the infected bladder were performed. it was found that continued bladder irrigations were necessary to stop bacterial ascension from the infected urethra. bladder capacity was increased in 6 of 8 patients with nonmalignant contracted bladder or interstitial cystitis. desinfection with so ... | 1976 | 176760 |
| clinical evaluation of a novel beta-lactam antibiotic: pivmecillinam (fl 1039). | pivmecillinam, a new penicillin-like antibiotic, is a member of the amidinopenicillanic acid group. its mode of action differs from that of the classical penicillins and it exhibits no cross-resistance with them. fifty-two gerontopsychiatric patients, median age 81 years, with e. coli, klebsiella, and proteus bacteriurias were divided into three comparable groups. in a ten week open clinical trial the patients were treated with pivmecillinam, pivampicillin or the two drugs given alternately in d ... | 1975 | 177372 |
| aerobic and anaerobic flora in bladder urine of patients with indwelling urethral catheters. | twenty-five bladder urine specimens from 13 patients with long-standing indwelling catheters were obtained by percutaneous suprapubic needle aspiration to avoid contamination by extraneous flora and cultured aerobically and anaerobically. twenty different species of organism were isolated from twenty-four positive specimens. the isolates were predominantly gram-negative aerobic or facultatively anaerobic bacilli, with occasional gram-positive aerobic cocci and yeasts. one patient had clostridium ... | 1976 | 178084 |
| in vivo synergy between 6 beta-amidinopenicillanic acid derivatives and other antibiotics. | both an oral and a parenteral form of a 6beta-amidinopenicillanic acid derivative were found to have appreciable activity against gram-negative bacteria and poor activity against gram-positive bacteria in vivo. when administered orally or parenterally, definite synergy was demonstrated between the amidinopenicillins and ampicillin, amoxicillin, benzylpenicillin, cefazolin, or carbenicillin in infections with a number of gram-negative bacteria, including klebsiella, enterobacter, escherichia, pro ... | 1976 | 178275 |
| lipids of klebsiella pneumoniae: the presence of phosphatidyl choline in succinate-grown cells. | the carbon and energy source for aerobically grown cultures of klebsiella pneumoniae profoundly influenced the total lipid content and phosphatide composition. glucose-grown cells contained 13% lipid, 56% of which was phospholipids. succinate-grown cells contained 8% lipid, 66% of which was phospholipids. the predominant phosphatides of glucose-grown cells were phosphatidyl ethanolamine, 82%; phosphatidyl glycerol, 4.5%; phosphatidic acid, 5%; cardiolipin, 6.5%; phosphatidyl serine; and trace am ... | 1976 | 178973 |
| the prosthetic group of citrate-lyase acyl-carrier protein. | the acyl carrier protein of citrate lyase contains adenine, phosphate, sugar, cysteamine, beta-alanine and pantoic acid in a molar ratio of 1:2:2:1:1:1. peptides containing these components in the same stoichiometric relationship were isolated after proteolytic digestion of acyl carrier protein. all components were linked together in a single prosthetic group. this was released from the peptide by mild alkaline hydrolysis. under these conditions a phosphodiester bond is cleaved which links the p ... | 1976 | 179809 |
| regulation of tyramine oxidase synthesis in klebsiella aerogenes. | tyramine oxidase in klebsiella aerogenes is highly specific for tyramine, dopamine, octopamine, and norepinephrine, and its synthesis is induced specifically by these compounds. the enzyme is present in a membrane-bound form. the km value for tyramine is 9 x 10(-4) m. tyramine oxidase synthesis was subjected to catabolite repression by glucose in the presence of ammonium salts. addition of cyclic adenosine 3',5'-monophosphate (camp) overcame the catabolite repression. a mutant strain, k711, whic ... | 1976 | 179974 |
| natural and altered induction of the l-fucose catabolic enzymes in klebsiella aerogenes. | mutants of klebsiella aerogenes w70 were isolated that had gained the ability to utilize the uncommon pentose d-arabinose as their sole source of carbon and energy. in contrast to the d-arabinose-negative, parent strain, these mutants were found to be either constitutive for certain enzymes of the l-fucose catabolic pathway or inducible for such enzymes when incubated in the presence of d-arabinose. the mutants used l-fucose isomerase to convert d-arabinose to d-ribulose, which is an intermediat ... | 1976 | 179982 |
| the cleavage of polyoma virus dna by restriction enzymes kpni and psti. | the action of two restriction endonucleases on polyoma virus dna has been examined and the sites at which they cleave the dna located. one of the enzymes, kpni from klebsiella pneumoniae ok8, cleaves polyoma dna twice at about 11-6 and 59-2% from the ecori site. the other enzyme, psti from providencia stuartii 164, cleaves polyoma dna five times at about 14-8, 16-5, 32-6, 50-3 and 80-0% from the ecori site. some of the cleavages produced by these enzymes alone, or in conjunction with other endon ... | 1976 | 180247 |
| structure of the prosthetic group of klebsiella aerogenes citrate (pro-3s)-lyase. | the prosthetic group of citrate (pro-3s)-lyase [citrate oxaloacetate-lyase (pro-3s-ch2coo- leads to acetate); ec 4.1.3.6] from klebsiella aerogenes was obtained by mild alkaline hydrolysis of the enzyme and purified by deae-cellulose chromatography. several chemical and enzymatic degradation products of the compound have been isolated, and analyses of these have shown the structure of the prosthetic group to be 3'(or 2') leads to 1 inch-(5 inches-phosphoribosyl) dephosphocoenzyme a. proof as to ... | 1976 | 180526 |
| [infections during malignant blood diseases. value of the carbenicillin-gentamicin combination]. | the association of carbenicillin and gentamicin was used as a routine in the early treatment of 48 attacks of fever, in 38 patients who mostly had acute leukemia and marrow aplasia. the attacks of infection may be classified as follows: 30% septicemia, 30% local infections, 40% no bacteria were identified. among the septicemias, we noted 5 septicemias due to pyocyaneus, 4 due to klebsiella. on the whole, in the attacks of fever, the association of carbenicillin and gentamicin proved effective in ... | 1975 | 180608 |
| isolation of pseudomonas aeruginosa and other bacterial species from ornamental aquarium plants. | ornamental aquarium plants were demonstrated to carry as many as 10(8) viable mesophilic bacteria per g. gram-negative organisms predominated among the 19 genera of bacteria identified. pseudomonas aeruginosa was readily isolated from 53% of the samples tested. | 1976 | 180888 |
| a general survey of the proton spin-lattice relaxation-times of some oligo- and poly-saccharide derivatives. | a fourier-transform method has been used to measure the spin-lattice relaxation-times (t1 values) of the anomeric protons of a selection of oligo- and poly-saccharide derivatives. although systematic variations are found for the substances of lower molecular weight, these variations are essentially non-existent at higher molecular weights. data for the disaccharides cellobiose, maltose, lactose, gentiobiose, and melibiose demonstrate that proton t1-values may provide a powerful method for evalua ... | 1976 | 183885 |
| susceptibility of "enterobacteria" to aminoglycoside antibiotics: comparisons with tetracyclines, polymyxins, chloramphenicol, and spectinomycin. | strains of enterobacteria, including common, aerobic, pathogenic gram-negative bacilli, and enterococci were tested for susceptibility to 11 aminoglycoside antibiotics by a twofold agar-dilution method with an inocula replicator. for comparison, similar tests were done with seven tetracycline analogues, two polymyxins, chloramphenicol, and spectinomycin. tobramycin compared favorably with the more active aminoglycosides, with some exceptions related to individual strains of species. the tetracyc ... | 1976 | 184213 |
| chirality of the hydrogen transfer to the coenzyme catalyzed by ribitol dehydrogenase from klebsiella pneumoniae and d-mannitol 1-phosphate dehydrogenase from escherichia coli. | the stereochemistry of the hydrogen transfer to nad catalyzed by ribitol dehydrogenase (ribitol:nad 2-oxidoreductase, ec 1.1.1.56) from klebsiella pneumoniae and d-mannitol-1-phosphate dehydrogenase (d-mannitol-1-phosphate:nad 2-oxidoreductase, ec 1.1.1.17) from escherichia coli was investigated. [4-3h]nad was enzymatically reduced with nonlabelled ribitol in the presence of ribitol dehydrogenase and with nonlabelled d-mannitol 1-phosphate and d-mannitol 1-phosphate dehydrogenase, respectively. ... | 1976 | 185137 |
| inter- and intralaboratory variability in antibiotic susceptibility tests with pseudomonas aeruginosa and enterobacteriaceae. | a two-phase collaboratice study was designed for definition of the extent to which results of disk diffusion tests can be reproduced. two currently recommended techniques, the kirby-bauer method and the agar overlay method, were used. of special concern were tests of pseudomonas aeruginosa with moderate susceptibility to carbenicillin; gentamicin, tobramycin, polymyxin b, and colistin were also tested with p. aeruginosa as well as with klebsiella pneumoniae, escherichia coli, and proteus species ... | 1976 | 185302 |
| chirality of the hydrogen transfer to nad catalyzed by myo-inositol dehydrogenase from klebsiella pneumoniae. | chirality, hydrogen transfer, myo-inositol dehydrogenase. the chirality of the hydrogen transfer to nad catalyzed by myo-inositol dehydrogenase (myo-inositol: nad 2-oxydoreductase, ec 1.1.1.18) from klebsiella pneumoniae (formerly classified taxonomically as aerobacter aerogenes or kleb siella aerogenes) was investigated. [4-3h] nad was enzymatically reduced to [4-3h] nadh with non-labeled myoinositol and myo-inositol dehydrogenase. the stereochemistry of the prochiral center at c4 of the nadh p ... | 1976 | 185832 |
| enzymic activities of cadmium- and zinc-tolerant strains of klebsiella (aerobacter) aerogenes growing in glucose-limited chemostats. | the activities of phosphatases and some enzymes of glucose metabolism were determined in k. aerogenes ncib 418 and in two strains derived from it, resistant to 50 mug cd2+ ml-1 and 16 mug zn2+ ml-1 respectively, during growth at d = 0.38 h-1 in medium containing beta-glycerophosphate as sole phosphorus source and supplemented with cd2+ and zn2+, as appropriate, for the resistant strains. the ph-activity profiles of the phosphatases differed from strain to strain but all showed maximum activity a ... | 1976 | 186694 |
| treatment with gentamicin monitored by serum antibiotic assay. | gentamicin is very widely accepted as the most effective antibiotic for treatment of a number of serious gram-negative infections, but one disadvantage associated with its use is the risk of toxicity particularly affecting the eighth nerve. an account is given of eight years' experience with this antibiotic in which obvious ototoxicity was avoided in hospitalized patients in whom gentamicin treatment was monitored by assay of the residual amounts of antibiotic present in the serum. the relations ... | 1976 | 186696 |
| [spondylodiskitis caused by klebsiella pneumoniae, an unusual complication of diverticular sigmoditis]. | | 1976 | 188196 |
| [microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses]. | the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ... | 1976 | 190825 |
| [changes in time of the microbial flora in bronchopulmonary infections]. | the authors carried out a study on 100 cases with broncho-pulmonary infections in two different periods: 1963--1964 and 1973--1974. changes in the microbial flora were investigated, as well as the sensitivity of germs to antibiotics. an increase was noted in the number of chronic bronchitis and a decrease in the number of pneumonia cases. in the microbial flora there was a constant proportion of staphylococcus, streptococcus and coli strains. the proportion of micrococci decreased with time and ... | 1977 | 191892 |
| anaerobic bacteria in biliary disease in elderly patients. | gallbladder bile from 52 elderly subjects who had undergone biliary tract surgery was examined for the presence of bacteria. twelve patients had sterile bile, 18 specimens of bile yielded anaerobes as well as aerobes, and 22 yielded aerobic bacteria only. escherichia coli was the most commonly isolated organism (30 strains). bacteroides fragilis was the most frequently encountered anaerobic bacterium and was found in 15 patients. the klebsiella-enterobacter group was the second most commonly iso ... | 1977 | 192815 |
| expression of klebsiella nif and his genes in salmonella typhimurium. | derivatives of salmonella typhimurium carrying f prime or p prime plasmids with klebsiella nif and his genes had specific nitrogenase activities similar to klebsiella in selective conditions, even to showing "hyperinduction" under argon. no evidence was obtained for catabolite repression of normal nif expression but dibutyl cyclic amp often augmented "hyperinduction". in non-selective conditions the klebsiella his nif determinants were rapidly lost from the plasmids; the low levels of nif expres ... | 1977 | 192833 |
| [bacteriological studies on mecillinam (author's transl)]. | | 1977 | 192923 |
| synthesis and some biological properties of 6-aminopenicillanic derivatives. | the properties of 33 6-aminopenicillanic derivates were described, the majority of which and particularly all 3,4-disubstituted phenyl derivatives, are new compounds. these derivatives were synthesized by three different methods consisting in treating of 6-aminopenicillanic acid (6-apa) with appropriate phenylthio -- and phenoxyalkanecarboxylic acids. the method consisting in use of aryloxyketenes is new and claimed by the patent applications. all synthesized compounds were screened for their bi ... | 1977 | 193092 |
| [etiology of acute respiratory infection in hospitalized children (author's transl)]. | incidence of respiratory tract infection represents 23% of the total number of admissions between 1-24 months of age, during a period of 18 months. the diagnosis were: bronchiolities, 143 cases; bronchopneumonia, 134 cases; tracheobronchitis, 50 cases; laryngitis, four cases, and bacterial pneumonia, 61 cases. monthly incidence was maximal in december of each year. from the total group, 144 cases were included in the present study to determine etiology of the infection. in 19% of the cases a ser ... | 1976 | 193420 |
| structural investigation of klebsiella serotype k36 polysaccharide. | klebsiella d36 capsular polysaccharide has been investigated by methylation, smith-periodate oxidation and partial hydrolysis techniques. the structure was found to consist of a hexasaccharide repeating unit as shown. the anomeric configurations of the sugar residues were determined by 1h and 13cn.m.r.spectroscopy on isolated oligomers obtained during the degradative studies and on the intact polysaccharide. | 1977 | 193645 |
| [studies on the antimicrobial effect of vitamin d (author's transl)]. | in in vitro studies vitamin d3 proved inhibitory on strains of staphylococcus aureus, streptococcus pyogenes, klebsiella pneumoniae. escherichia coli, and candida albicans. in the presence of 5x10(4)-9x10(4) iu/ml vitamin d3 the organisms were killed or reacted with a marked growth inhibition. | 1977 | 195120 |
| bacteriology of the human biliary tract and the duodenum. | using the modern anaerobic transport media and meticulous culture techniques, 74 patients undergoing biliary tract surgery were studied. the biliary system was found to be sterile in 58 patients (78%). fifteen patients had 35 isolates of aerobic and facultative bacteria. the most common ones were klebsiella, enterococcus, and escherichia coli. the only anaerobe isolated was clostridium perfringens. eight of 17 patients (47%) with acute cholecystitis and five of 49 patients (10%) with chronic cho ... | 1977 | 195558 |
| on the structure of the prosthetic group of citrate (pro-3s)-lyase. | the prosthetic group of citrate (pro-3s)-lyase from klebsiella aerogenes as well as streptococcus diacetilactis was obtained eigher by beta elimination or pronase digestion of the enzyme and purified by deae-cellulose chromatography. the compound was shown to contain 3 mol of po4, 2 mol of ribose, and 1 mol of sulfhydryl/mol of adenine. 5'-amp and dephospho-coa are components of the prosthetic group. the evidence obtained so far support our proposed structure of 3' (or 2') leads to 1''-(5''-phos ... | 1977 | 197081 |
| [specific endonuclease of klebsiella pneumoniae oks]. | | 1977 | 199404 |
| anaerobes in human biliary tracts. | during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ... | 1977 | 200634 |
| spontaneous infectious diseases of marmosets. | the various species of marmosets are susceptible to a wide variety of infectious agents of which only a few have been fully characterized. little is known concerning spontaneous disease in their natural habitat, and often deaths in the laboratory go unexplained. in captivity, herpesvirus-t infection appears to be the most important viral infection, but serious disease may also follow infection with measles virus (rubeola) and an unidentified paramyxovirus. bacterial diseases are multiple, but ra ... | 1978 | 205855 |
| subcellular distribution of enzymes involved in alpha-glucan utilization in klebsiella pneumoniae. proteins of cytopasm, periplasm, cytoplasmic and outer membrane. | | 1978 | 206221 |
| electron paramagnetic resonance studies on membrane-bound respiratory nitrate reductase of klebsiella aerogenes. | | 1978 | 207577 |
| coenzyme b12-dependent diol dehydratase: regulation of apoenzyme synthesis in klebsiella pneumoniae (aerobacter aerogenes) atcc 8724. | immunochemical studies demonstrated that klebsiella pneumoniae (aerobacter aerogenes) atcc 8724 produces only a single diol dehydratase whether grown on glycerol or on 1,2-propanediol. the enzyme was subject to induction by 1,2-diols and to catabolite repression reversed by cyclic amp. | 1978 | 210157 |
| electron-paramagnetic-resonance studies on nitrogenase of klebsiella pneumoniae. evidence for acetylene- and ethylene-nitrogenase transient complexes. | klebsiella pneumoniae nitrogenase exhibited four new electron-paramagnetic-resonance signals during turnover at 10 degrees c, ph7.4, which were assigned to intermediates present in low concentrations in the steady state. 57fe-substituted mo--fe protein showed that they arose from fe--s clusters in the mo--fe protein of nitrogenase. the new signals are designated: ic, g values at 4.67, 3.37 and approx. 2.0; vi, g values at 2.125, 2.000 and 2.000; vii, g values at 5.7 and 5.4; viii, g values at 2. ... | 1978 | 210766 |
| experimental evolution in bacteria. | | 1978 | 212253 |
| nitrogenase x: mössbauer and epr studies on reversibly oxidized mofe protein from azotobacter vinelandii op. nature of the iron centers. | under anaerobic conditions the molybdenum-iron protein (mofe protein) from azotobacter vinelandii can be reversibly oxidized with thionine. electron paramagnetic resonance studies reveal that the oxidation proceeds in two distinct phases: the mofe protein can be oxidized by four electrons without loss of the epr signal from the s = 3/2 cofactor centers. a second oxidation step, involving two electrons, leads to the disappearance of the cofactor epr signal. in order to correlate the events during ... | 1978 | 215215 |
| stopcock: bacterial contamination in invasive monitoring systems. | | 1979 | 215570 |
| role of peripheral side chains of vitamin b12 coenzymes in the reaction catalyzed by dioldehydrase. | | 1979 | 217415 |
| clostridia as pathogens in neonatal necrotizing enterocolitis. | | 1979 | 221640 |
| "triple infections" (fungal, bacterial and viral) in immunosuppressed renal transplant recipients. | in a period of 5 years, 8 out of 77 renal transplant patients showed simultaneous fungal, bacterial and viral infections. candida albicans was found in all cases. the most severe bacterial complications were infections with klebsiella, pseudomonas and staphylococcus aureus. cytomegalovirus, persistent hbsag positive hepatitis, herpes zoster, and herpes simplex infections were also found. seven patients died of bacterial superinfection and miliary tuberculosis. the data presented show that "tripl ... | 1979 | 224003 |
| [effects of ampicillin and cefazolin combined with mecillinam (author's transl)]. | combination effects of mecillinam + ampicillin and mecillinam + cefazolin were investigated by the cross diffusion test and by the kinetics of bactericidal action. synergism was found by cross diffusion in about 50% of strains of e. coli, klebsiella and proteus. sensitivity against ampicillin was correlated with synergism. in mecillinam-resistant strains, synergism is usually absent. the combination of ampicillin or cefazolin with mecillinam leads to bactericidal acceleration; the effects are co ... | 1978 | 226099 |
| in vitro combination of mecillinam with cephradine or amoxycillin for organisms resistant to single agents. | of 36 multi-resistant gram-negative bacilli isolated from different cases of urinary tract infection, 18 strains of escherichia coli, klebsiella, enterobacter, proteus, providencia and serratia with the exception of acinetobacter species showed synergy when mecillinam was combined with cephradine or amoxycillin in the ratio of 1:1. combination with cephradine was synergistic on sixteen occasions and amoxycillin on six. synergy, which might have clinical relevance, was demonstrated only when the ... | 1979 | 227829 |
| mecillinam-ampicillin synergism in experimental enterobacteriaceae meningitis. | the in vitro activities of mecillinam, a new beta-amidinopenicillin, and ampicillin, alone and in combination, against an escherichia coli strain and a klebsiella pneumoniae strain were compared, and these results were correlated with their respective activities in vivo in experimental meningitis. the mecillinam-ampicillin combination was synergistic in vitro against both strains when tested by a modified checkerboard technique (bacteriostatic synergy). however when quantitative bactericidal syn ... | 1979 | 228591 |
| lethal effect of complement and lysozyme on polymyxin-treated, serum-resistant gram-negative bacilli. | when genetically serum-resistant escherichia coli, klebsiella pneumoniae, and citrobacter freundii, but not pseudomonas aeruginosa or proteus mirabilis, were exposed to polymyxin b, they became susceptible to the bactericidal action of normal human and rabbit sera. in constrast, beta-lactam and aminoglycoside antibiotics did not render any serum-resistant bacteria serum-sensitive. synergy between polymyxin b and the serum bactericidal system could be demonstrated by the addition of polymyxin b t ... | 1979 | 229175 |
| investigations into the klebsiella aerogenes pentitol operons using specialised transducing phages lambdaprbt and lambdaprbt dal. | | 1979 | 230352 |
| specificity of attachment of certain enterobacteriaceae to mammalian cells. | the specificity of adherence of various enterobacteriaceae to different mammalian cells was studied in vitro. 3h-labelled organisms of the same species isolated from various clinical sources differed significantly in their abilities to adhere to the same mammalian cells. bacteria frequently adhered better to cells derived from sites other than those analogous to their original source. bacteria did not display consistently 'high' or 'low' attachment to a variety of human and tissue-cultured cells ... | 1979 | 231084 |
| cloning of the entire region for nitrogen fixation from klebsiella pneumoniae on a multicopy plasmid vehicle in escherichia coli. | | 1979 | 232231 |
| prolonged cultures of entamoeba histolytica. | | 1979 | 232687 |
| changes in protein synthesis on mitomycin c induction of wild-type and mutant clodf13 plasmids. | mitomycin c treatment of escherichia coli k-12 cells containing the nonconjugative plasmid clodf13 resulted in inhibition of host chromosome protein synthesis and a high rate of synthesis of two clodf13-specified proteins whose molecular weights correspond to cloacin and immunity protein. five molecules of immunity protein were synthesized for each cloacin df13 molecule. mitomycin c-treated cells containing a copy mutant of clodf13 made three to four times as much of each protein as cells contai ... | 1977 | 233723 |
| in vitro activity of sisomicin, an aminoglycoside antibiotic, against clinical isolates. | sisomicin, a new aminoglycoside antibiotic which is produced by micromonospora myoensis, was studied against 565 clinical isolates of gram-negative bacilli and gram-positive cocci. with the exception of serratia marcescens, over 90% of isolates of gram-negative bacilli were inhibited by 1.56 mug/ml or less of sisomicin. sisomicin was slightly more active than gentamicin and tobramycin aganist isolates of escherichia coli, proteus mirabilis and klebsiella spp. it was substantially more active tha ... | 1975 | 234415 |
| [clinico-bacteriological correlations in patents with chronic nonspecific diseases of the lungs]. | | 1975 | 234629 |
| purification, structure and properties of the respiratory nitrate reductase of klebsiella aerogenes. | 1. the respiratory nitrate reductase of klebsiella aerogenes was solubilized from the bacterial membranes by deoxycholate and purified further by means of gel chromatography in the presence of deoxycholate, and anion-exchange chromatography. 2. dependent on the isolation procedure two different homogeneous forms of the enzyme, having different subunit compositions, can be obtained. these forms are designated nitrate reductase i and nitrate reductase ii. both enzyme preparations are isolated as t ... | 1975 | 234757 |
| identification of the structural gene for glutamine synthetase in klebsiella aerogenes. | mutations at two sites of the klebsiella aerogenes chromosome, unlinked by transduction with phages pw52 and p1, result in the lack of enzymatically active glutamine synthetase. a mutation in the glnb site leads to a marked decrease in the formation of an apparently normal enzyme. some of the mutations in the glna site lead to the production of enzymatically inactive material capable of reacting with anti-glutamine synthetase serum. the revertant of a glna mutant was found to produce a glutamine ... | 1975 | 234938 |
| genetic control of glutamine synthetase in klebiella aerogenes. | mutations at two sites, glna and glnb, of the klebsiella aerogenes chromosome result in the loss of glutamine synthetase. the locations of these sites on the chromosome were established by complementation by episomes of escherichia coli and by determination of their linkage to other genetic sites by transduction with phage p1. the glnb gene is located at a position corresponding to 48 min on the taylor map of the e. coli chromosome; it is linked to trya, nadb, and gua. the glna gene is at a posi ... | 1975 | 234939 |
| 1-alkyl-2-nitroimidazol-5-yl derivatives. i. | | 1975 | 235267 |
| a three year retrospective analysis of septic shock in a general hospital. | records of all patients with a clinical diagnosis of septic shock were reviewed retrospectively; cases occurred from august 1968-july 1971 in a general teaching hospital with 667 beds. in the 3-year period, 80 patients (38 males and 42 females) presented with 82 episodes of septic shock; this represented 14% of total hospital admissions to intensive care. the age range was from 15-78 years, with a mean of 52+ or -16 years. the incidence of the syndrome increased with age, reaching a maximum i ... | 1975 | 235790 |
| the classic bacterial pneumonias. | | 1975 | 236155 |
| effect of aeration and sodium on the metabolism of citrate by klebsiella aerogenes. | anaerobic growth of klebsiella aerogenes ncdo 711 (nctc 418) on citrate was dependent on the presence of na+ in the medium, and fermentation of citrate was mediated via the fermentation pathway enzymes, citrate lyase and a na+-dependent oxalacetate decarboxylase. this confirms the previous findings on strain nctc 418. growth under aerobic conditions was independent of na+. the mean generation time for cells grown aerobically on either na+ or k+ citrate medium was about 60 min, with a molar growt ... | 1975 | 236280 |
| nationwide epidemic of septicemia caused by contaminated infusion products. iv. growth of microbial pathogens in fluids for intravenous infusions. | septicemia caused by contaminated infusion fluid is a newly appreciated hazard of intravenous infusion therapy. microorganisms of the tribe klebsielleae (klebsiella, enterobacter, and serratia) have predominated in these infections. members of this tribe found to possess a selecive ability over common non-klebsielleae microbial pathogens to proliferate rapidly in commerical parential fluids contaning clucose at room temperautre. fifty-one klebsielleae strains, washed twice before inoculation of ... | 1975 | 236343 |
| impedance monitoring of bacterial activity. | bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. the signal is expressed automatically as a curve similar to grwoth curves produced by other methods. the technique offers a new, rapid and sensitive means of detecting active micro-organisms and is potentially the basis of rapid automated systems in this field. the impedance changes indicate that the micro-organisms metabolise substrates of low conductivity into products of ... | 1975 | 236390 |
| evaluation of bacterial contamination in blood processing. | in the routine deglycerolization of frozen red blood cells, a bottle of solution intended as a final wash (0.8% nacl, 0.2% dextrose) was found to be abnormally colored and slightly turbid. close inspection revealed a small crack in the base of the bottle. a pure growth of klebsiella pneumoniae was cultured. the rabbit pyrogen test on the solution was positive using 2.5 mul as was the limulus amebocyte lysate (lal) test at a dilution of 1:10-5 for endotoxin. studies of the growth of this organism ... | 1975 | 236609 |
| long-term therapy of chronic bacterial prostatitis with trimethoprim-sulfamethoxazole. | trimethoprim (tmp) meets all of the theoretical requirements of diffusion into, and actual concentration in, human prostatic fluid. when tmp is combined with the sulfonamide sulfamethoxazole (smx), potentiation of antibacterial activity is achieved and the development of resistant bacterial strains is less likely to occur. in our initial use of tmp-smx in the treatment of 13 men with chronic bacterial prostatitis due to gram-negative organisms, patients were given two tablets of tmp-smx twice da ... | 1975 | 236820 |
| the structure of klebsiella serotype ii capsular polysaccharide. | using periodate oxidation, methylation analysis, the characterization of oligosaccharides obtained by partial acid hydrolysis, p.m.r. spectroscopy, and analytical ultracentrifugation, the structure of the (mildly alkali-treated) klebsiella serotype 11 capusular polysaccharide has been elucidated. the tetrasaccharide repeating-unit comprises the sequence yields 3)-beta-d-glcp-(1 yields 3)-beta-d-glcuap-(1 yields 3)-alpha-d-galp-(1 yields with a 4,6-o-(1-carboxyethylidene)-alpha-d-galactosyl resid ... | 1975 | 236829 |
| bacteriophage-borne enzymes in carbohydrate chemistry. part i. on the glycanase activity associated with particles of klebsiella bacteriophage no. 11. | the preparation and use of particles of klebsiella bacteriophage no. 11 are described. a glycanase activity associated with the viruses catalyses the depolymerization of (alkali-treated) klebsiella serotype 11 capsular polysaccharide, ultimately to a mixture of oligosaccharides consisting of one or two repeating units. mainly glucosidic bonds are hydrolysed. the substrate specificity of the viral enzyme has been characterized by using derivatives of serotype-11 polysaccharide, as well as 81 hete ... | 1975 | 236830 |