| differences in two-component signal transduction proteins among the genus brucella: implications for host preference and pathogenesis. | two-component systems (tcss) are the predominant bacterial signal transduction mechanisms. species of the genus brucella are genetically highly related and differ mainly in mammalian host adaptation and pathogenesis. in this study, tcs proteins encoded in the available genome sequences of brucella species have been identified using bioinformatic methods. all the brucella species share an identical set of tcs proteins, and the number of tcs proteins in the closely related opportunistic human path ... | 2010 | 20153589 |
| changes in denitrifier abundance, denitrification gene mrna levels, nitrous oxide emissions, and denitrification in anoxic soil microcosms amended with glucose and plant residues. | in agricultural cropping systems, crop residues are sources of organic carbon (c), an important factor influencing denitrification. the effects of red clover, soybean, and barley plant residues and of glucose on denitrifier abundance, denitrification gene mrna levels, nitrous oxide (n(2)o) emissions, and denitrification rates were quantified in anoxic soil microcosms for 72 h. nosz gene abundances and mrna levels significantly increased in response to all organic carbon treatments over time. in ... | 2010 | 20154105 |
| an atypical riboflavin pathway is essential for brucella abortus virulence. | brucellosis is a worldwide zoonosis that affects livestock and humans and is caused by closely related brucella spp., which are adapted to intracellular life within cells of a large variety of mammals. brucella can be considered a furtive pathogen that infects professional and non-professional phagocytes. in these cells brucella survives in a replicative niche, which is characterized for having a very low oxygen tension and being deprived from nutrients such as amino acids and vitamins. among th ... | 2010 | 20195542 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |
| tightly regulated expression vectors for ochrobactrum anthropi. | genetic studies of ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. a tightly regulated gene-expression system was developed for o. anthropi using the laci(q) gene and a re-engineered coliphage t5 promoter containing completely symmetrical dna segment that binds more efficiently to the lactose repressor. the beta-galactosidase activity was increased 57-fold when the expressi ... | 2010 | 20237980 |
| beta-aminopeptidase-catalyzed biotransformations of beta(2)-dipeptides: kinetic resolution and enzymatic coupling. | we have previously shown that the beta-aminopeptidases bapa from sphingosinicella xenopeptidilytica and dmpa from ochrobactrum anthropi can catalyze reactions with non-natural beta(3)-peptides and beta(3)-amino acid amides. here we report that these exceptional enzymes are also able to utilize synthetic dipeptides with n-terminal beta(2)-amino acid residues as substrates under aqueous conditions. the suitability of a beta(2)-peptide as a substrate for bapa or dmpa was strongly dependent on the s ... | 2010 | 20340152 |
| a novel hydrolytic dehalogenase for the chlorinated aromatic compound chlorothalonil. | dehalogenases play key roles in the detoxification of halogenated aromatics. interestingly, only one hydrolytic dehalogenase for halogenated aromatics, 4-chlorobenzoyl-coenzyme a (coa) dehalogenase, has been reported. here, we characterize another novel hydrolytic dehalogenase for a halogenated aromatic compound from the 2,4,5,6-tetrachloroisophthalonitrile (chlorothalonil)-degrading strain of pseudomonas sp. ctn-3, which we have named chd. chd catalyzes a hydroxyl substitution at the 4-chlorine ... | 2010 | 20363940 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| subcellular localization and characterization of the parab system from corynebacterium glutamicum. | faithful segregation of chromosomes and plasmids is a vital prerequisite to produce viable and genetically identical progeny. bacteria use a specialized segregation system composed of the partitioning proteins para and parb to segregate certain plasmids. strikingly, homologues of para and parb are found to be encoded in many chromosomes. although mutations in the chromosomal par system have effects on segregation efficiency, the exact mechanism by which the chromosomes are segregated into the da ... | 2010 | 20435732 |
| aidh, an alpha/beta-hydrolase fold family member from an ochrobactrum sp. strain, is a novel n-acylhomoserine lactonase. | n-acylhomoserine lactones (ahls) are signaling molecules in many quorum-sensing (qs) systems that regulate interactions between various pathogenic bacteria and their hosts. quorum quenching by the enzymatic inactivation of ahls holds great promise in preventing and treating infections, and several such enzymes have been reported. in this study, we report the characterization of a novel ahl-degrading protein from the soil bacterium ochrobactrum sp. strain t63. this protein, termed aidh, shares no ... | 2010 | 20525860 |
| peritonitis in type 2 diabetes mellitus due to ochrobactrum anthropi complicating automated peritoneal dialysis. | epidemiology data predict that by the year 2025, diabetes will affect about 380 million people worldwide with a significant increase in patients with chronic renal disease progressing to hemodialysis. diabetes-related peripheral vascular disease is a major risk factor for vascular access failure in patients on extracorporeal hemodialysis. although peritoneal dialysis is a valid option for diabetics, peritonitis is still a main complication for these patients. we report the case of a 71-year-old ... | 2010 | 20563608 |
| osmotically induced synthesis of the dipeptide n-acetylglutaminylglutamine amide is mediated by a new pathway conserved among bacteria. | the dipeptide n-acetylglutaminylglutamine amide (naggn) was discovered in the bacterium sinorhizobium meliloti grown at high osmolarity, and subsequently shown to be synthesized and accumulated by a few osmotically challenged bacteria. however, its biosynthetic pathway remained unknown. recently, two genes, which putatively encode a glutamine amidotransferase and an acetyltransferase and are up-regulated by osmotic stress, were identified in pseudomonas aeruginosa. in this work, a locus carrying ... | 2010 | 20571117 |
| bioremediation of cr(vi) and immobilization as cr(iii) by ochrobactrum anthropi. | bioremediation of cr(vi) through reduction relies on the notion that the produced cr(iii) may be precipitated or efficiently immobilized. however, recent reports suggest that soluble organo-cr(iii) complexes are present in various chromate-reducing bacterial systems. this work was designed to explore the factors that affect the immobilization of cr(iii) in the ochrobactrum anthropi system. x-ray absorption fine structure analysis on the cell debris clearly verified that coordination of cr(iii) o ... | 2010 | 20608725 |
| a new family of enzymes catalyzing the first committed step of the methylerythritol 4-phosphate (mep) pathway for isoprenoid biosynthesis in bacteria. | isoprenoids are a large family of compounds with essential functions in all domains of life. most eubacteria synthesize their isoprenoids using the methylerythritol 4-phosphate (mep) pathway, whereas a minority uses the unrelated mevalonate pathway and only a few have both. interestingly, brucella abortus and some other bacteria that only use the mep pathway lack deoxyxylulose 5-phosphate (dxp) reductoisomerase (dxr), the enzyme catalyzing the nadph-dependent production of mep from dxp in the fi ... | 2010 | 20660776 |
| bioremediation of glyphosate-contaminated soils. | based on the results of laboratory and field experiments, we performed a comprehensive assessment of the bioremediation efficiency of glyphosate-contaminated soddy-podzol soil. the selected bacterial strains achromobacter sp. kg 16 (vkm b-2534d) and ochrobactrum anthropi gpk 3 (vkm b-2554d) were used for the aerobic degradation of glyphosate. they demonstrated high viability in soil with the tenfold higher content of glyphosate than the recommended dose for the single in situ treatment of weeds. ... | 2010 | 20676632 |
| genomic comparisons of brucella spp. and closely related bacteria using base compositional and proteome based methods. | classification of bacteria within the genus brucella has been difficult due in part to considerable genomic homogeneity between the different species and biovars, in spite of clear differences in phenotypes. therefore, many different methods have been used to assess brucella taxonomy. in the current work, we examine 32 sequenced genomes from genus brucella representing the six classical species, as well as more recently described species, using bioinformatical methods. comparisons were made at t ... | 2010 | 20707916 |
| herbaspirillum species bacteremia in a pediatric oncology patient. | herbaspirillum species, an organism commonly found in soil, has only recently been linked to disease in humans. we report herbaspirillum bacteremia in a 2-year-old female patient following a hematopoietic stem cell transplant for relapsed acute lymphoblastic leukemia. | 2010 | 20739483 |
| bacterial bloodstream infections in hiv-infected adults attending a lagos teaching hospital. | an investigation was carried out during october 2005-september 2006 to determine the prevalence of bloodstream infections in patients attending the outpatient department of the hiv/aids clinic at the lagos university teaching hospital in nigeria. two hundred and one patients--86 males and 115 females--aged 14-65 years were recruited for the study. serological diagnosis was carried out on them to confirm their hiv status. their cd4 counts were done using the micromagnetic bead method. twenty ml o ... | 2010 | 20824974 |
| characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses. | diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ... | 2011 | 20859703 |
| [ochrobactrum anthropi and polymicrobial peritonitis in peritoneal dialysis: a resistance predictor]. | | 2010 | 20882099 |
| differential phenotyping of brucella species using a newly developed semi-automated metabolic system. | a commercial biotyping system (taxa profile™, merlin diagnostika) testing the metabolization of various substrates by bacteria was used to determine if a set of phenotypic features will allow the identification of members of the genus brucella and their differentiation into species and biovars. | 2010 | 20969797 |
| heat resistance mediated by a new plasmid encoded clp atpase, clpk, as a possible novel mechanism for nosocomial persistence of klebsiella pneumoniae. | klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. we have characterized a k. pneumoniae strain responsible for a series of critical infections in an intensive care unit over a two-year period. the strain was found to be remarkably thermotolerant providing a conceivable explanation of its persistence in the hospital environment. this marked phenotype is mediated by a novel type of clp atpase, designated clpk. the clpk gene is encoded by a ... | 2010 | 21085699 |
| biliary sepsis caused by ochrobactrum anthropi. | ochrobactrum anthropi is an emerging pathogen in immunocompromised patients, with the majority of human cases being central venous catheter-related infections. in contrast, o. anthropi-related biliary sepsis is much rare. herein we report the clinical and microbiological characteristics of o. anthropi-related biliary sepsis in order to increase awareness of the potential role of o. anthropi in this infection. further extensive epidemiologic studies should be carried out to ascertain the etiologi ... | 2010 | 21099098 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16s rrna gene sequencing for identification of difficult-to-identify bacterial strains. | conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16s rrna gene sequencing. maldi-tof ms might represent an alternative to 16s rrna gene sequencing. | 2010 | 21106794 |
| hydroxylated ornithine lipids increase stress tolerance in rhizobium tropici ciat899. | ornithine lipids (ols) are widespread among gram-negative bacteria. their basic structure consists of a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino group of ornithine and a second fatty acyl group ester-linked to the 3-hydroxy position of the first fatty acid. ols can be hydroxylated within the secondary fatty acyl moiety and this modification has been related to increased stress tolerance. rhizobium tropici, a nodule-forming α-proteobacterium known for its stress toleran ... | 2011 | 21205018 |
| ribosomal rna sequence analysis of brucella infection misidentified as ochrobactrum anthropi infection. | a brucella isolate was identified from purulent material collected during a hip surgery. two previous blood cultures from the same patient yielded ochrobactrum anthropi. after rrna sequencing, all the isolates were identified as brucella species and subsequently serotyped as brucella suis. misidentification of brucella species remains a problem with bacterial identification systems. | 2011 | 21209167 |
| plasmids with a chromosome-like role in rhizobia. | replicon architecture in bacteria is commonly comprised of one indispensable chromosome and several dispensable plasmids. this view has been enriched by the discovery of additional chromosomes, identified mainly by localization of rrna and/or trna genes, and also by experimental demonstration of their requirement for cell growth. the genome of rhizobium etli cfn42 is constituted by one chromosome and six large plasmids, ranging in size from 184 to 642 kb. five of the six plasmids are dispensable ... | 2011 | 21217003 |
| bacterial genome chimaerism and the origin of mitochondria. | many studies have sought to determine the origin and evolution of mitochondria. although the alphaproteobacteria are thought to be the closest relatives of the mitochondrial progenitor, there is dispute as to what its particular sister group is. some have argued that mitochondria originated from ancestors of the order rickettsiales, or more specifically of the rickettsiaceae family, while others believe that ancestors of the family rhodospirillaceae are also equally likely the progenitors. to re ... | 2011 | 21217797 |
| simple enzymatic procedure for l-carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recycling. | β-peptides and their derivates are usually stable to proteolysis and have an increased half-life compared with α-peptides. recently, β-aminopeptidases were described as a new enzyme class that enabled the enzymatic degradation and formation of β-peptides. as an alternative to the existing chemical synthesis routes, the aim of the present work was to develop a whole-cell biocatalyst for the synthesis and production of β-peptides using this enzymatic activity. for the optimization of the reaction ... | 2010 | 21255308 |
| simple enzymatic procedure for l-carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recycling. | β-peptides and their derivates are usually stable to proteolysis and have an increased half-life compared with α-peptides. recently, β-aminopeptidases were described as a new enzyme class that enabled the enzymatic degradation and formation of β-peptides. as an alternative to the existing chemical synthesis routes, the aim of the present work was to develop a whole-cell biocatalyst for the synthesis and production of β-peptides using this enzymatic activity. for the optimization of the reaction ... | 2010 | 21255308 |
| putative atp-binding cassette transporter is essential for brucella ovis pathogenesis in mice. | brucella ovis is a major cause of reproductive failure in sheep, which is associated with epididymitis and infertility in rams. importantly, b. ovis is one of the few brucella species that is not zoonotic. due to the scarcity of studies on b. ovis infection, a murine model of infection was developed. the roles of b. ovis genes encoding a putative hemagglutinin and an abc transporter were investigated in the mouse model. the kinetics of b. ovis infection were similar in balb/c and c57bl/6 mice, a ... | 2011 | 21300772 |
| exoproteome of staphylococcus aureus reveals putative determinants of nasal carriage. | due to the increasing prevalence of nosocomial and community-acquired antibiotic resistant staphylococcus aureus (sa), understanding the determinants of sa nasal carriage has become a major imperative. previous research has revealed many host and bacterial factors that contribute to sa nasal carriage. to assess bacterial factors that facilitate nasal carriage, we compared the exoproteome of a nasal carrier strain of sa to a genetically similar noncarrier strain. additionally, the carrier strain ... | 2011 | 21338050 |
| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| multilocus sequence-based analysis delineates a clonal population of agrobacterium (rhizobium) radiobacter (agrobacterium tumefaciens) of human origin. | the genus agrobacterium includes plant-associated bacteria and opportunistic human pathogens. taxonomy and nomenclature within the genus remain controversial. in particular, isolates of human origin were all affiliated with the species agrobacterium (rhizobium) radiobacter, while phytopathogenic strains were designated under the synonym denomination agrobacterium tumefaciens. in order to study the relative distribution of agrobacterium strains according to their origins, we performed a multilocu ... | 2011 | 21398532 |
| s-glutathionyl-(chloro)hydroquinone reductases: a new class of glutathione transferases functioning as oxidoreductases. | glutathione transferases (gsts) are best known for transferring glutathione (gsh) to hydrophobic organic compounds, making the conjugates more soluble. however, the omega-class gsts of animals and the lambda-class gsts and dehydroascorbate reductases (dhars) of plants have little or no activity for gsh transfer. instead, they catalyze gsh-dependent oxidoreductions. the lambda-class gsts reduce disulfide bonds, the dhars reduce the disulfide bonds and dehydroascorbate, and the omega-class gsts ca ... | 2011 | 21425927 |
| housekeeping genes essential for pantothenate biosynthesis are plasmid-encoded in rhizobium etli and rhizobium leguminosarum. | a traditional concept in bacterial genetics states that housekeeping genes, those involved in basic metabolic functions needed for maintenance of the cell, are encoded in the chromosome, whereas genes required for dealing with challenging environmental conditions are located in plasmids. exceptions to this rule have emerged from genomic sequence data of bacteria with multipartite genomes. the genome sequence of r. etli cfn42 predicts the presence of panc and panb genes clustered together on the ... | 2011 | 21463532 |
| bioinformatic characterization of the trimeric intracellular cation-specific channel protein family. | trimeric intracellular cation-specific (tric) channels are integral to muscle excitation-contraction coupling. tric channels provide counter-ionic flux when calcium is rapidly transported from intracellular stores to the cell cytoplasm. until recently, knowledge of the presence of these proteins was limited to animals. we analyzed the tric family and identified a profusion of prokaryotic family members with topologies and motifs similar to those of their eukaryotic counterparts. prokaryotic memb ... | 2011 | 21519847 |
| comparative activities of tigecycline and other tetracyclines against non-fermenting gram-negative bacilli (excluding acinetobacter spp.). | tigecycline, a glycylcycline, is a semisynthetic derivative of minocycline with a broad spectrum of activity against aerobic and anaerobic bacteria (2, 12, 16).… | 2011 | 21606223 |
| microbial degradation of microcystin in florida's freshwaters. | presence of microcystin (mc), a predominant freshwater algal toxin and a suspected liver carcinogen, in florida's freshwaters poses serious health threat to humans and aquatic species. being recalcitrant to conventional physical and chemical water treatment methods, biological methods of mc removal is widely researched. water samples collected from five sites of lake okeechobee (lo) frequently exposed to toxic microcystis blooms were used as inoculum for enrichment with microcystin lr (mc-lr) su ... | 2011 | 21611743 |
| microbial degradation of microcystin in florida's freshwaters. | presence of microcystin (mc), a predominant freshwater algal toxin and a suspected liver carcinogen, in florida's freshwaters poses serious health threat to humans and aquatic species. being recalcitrant to conventional physical and chemical water treatment methods, biological methods of mc removal is widely researched. water samples collected from five sites of lake okeechobee (lo) frequently exposed to toxic microcystis blooms were used as inoculum for enrichment with microcystin lr (mc-lr) su ... | 2011 | 21611743 |
| the utility of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) following introduction for routine laboratory bacterial identification. | maldi-tof ms was evaluated prospectively in a diagnostic laboratory. 927 organisms were tested in triplicate; 2351/2781 (85%) species and 2681/2781 (96%) genus identifications were correct. known issues such as the misidentification of α-haemolytic streptococci as streptococcus pneumonia were easily corrected. identifications cost $aud0.45 per isolate and were available in minutes. maldi-tof ms is rapid, accurate and inexpensive. | 2011 | 21632894 |
| new approaches to identification and activity estimation of glyphosate degradation enzymes. | we propose a new set of approaches, which allow identifying the primary enzymes of glyphosate (n-phosphonomethyl-glycine) attack, measuring their activities, and quantitative analysis of glyphosate degradation in vivo and in vitro. using the developed approach we show that glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains: in ochrobactrum anthropi gpk3 the initial cleavage reaction is catalyzed by glyphosate-oxidoreductase wit ... | 2011 | 21639854 |
| multiple loci are involved in quorum quenching of autoinducer i molecules in the nitrogen-fixing symbiont (sino-) rhizobium sp. ngr234. | rhizobium sp. ngr234 is a unique α-proteobacterium (order - rhizobiales) that forms nitrogen-fixing nodules with more legumes than any other micro-symbiont. since we have previously described the complete genome sequence of ngr234, we now report on a genome wide functional analysis of the genes and enzymes involved in autoinducer i hydrolysis in this microbe. altogether we identified five cosmid clones that repeatedly gave a positive result in our function-based approach for the detection of aut ... | 2011 | 21642401 |
| 16s rdna-based phylogeny of non-symbiotic bacteria of entorno-pathogenic nematodes from infected insect cadavers. | using 16s rdna gene sequencing technique, three different species of non-symbiotic bacteria of entomopatho-genic nematodes (epns) (steinernema sp. and heterorhabditis sp.) were isolated and identified from infected insect cadavers {galleria mellonella larvae) after 48-hour post infections. sequence similarity analysis revealed that the strains srk3, srk4 and srk5 belong to ochrobactrum cytisi, schineria larvae and ochrobactrum anthropi, respectively. the isolates o. anthropi and s. larvae were f ... | 2011 | 21802047 |
| distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria ochrobactrum anthropi and achromobacter sp. | bacterial strains capable of utilizing methylphosphonic acid (mp) or glyphosate (gp) as the sole sources of phosphorus were isolated from soils contaminated with these organophosphonates. the strains isolated from mp-contaminated soils grew on mp and failed to grow on gp. one group of the isolates from gp-contaminated soils grew only on mp, while the other one grew on mp and gp. strains achromobacter sp. mps 12 (vkm b-2694), mp degraders group, and ochrobactrum anthropi gpk 3 (vkm b-2554d), gp d ... | 2011 | 21789492 |
| genome of ochrobactrum anthropi atcc 49188t, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts. | ochrobactrum anthropi is a common soil alphaproteobacterium that colonizes a wide spectrum of organisms and is being increasingly recognized as an opportunistic human pathogen. potentially life-threatening infections, such as endocarditis, are included in the list of reported o. anthropi infections. these reports, together with the scant number of studies and the organism's phylogenetic proximity to the highly pathogenic brucellae, make o. anthropi an attractive model of bacterial pathogenicity. ... | 2011 | 21685287 |
| in search of brucella abortus type iv secretion substrates: screening and identification of four proteins translocated into host cells through virb system. | type iv secretion systems (t4ss) are specialized protein complexes used by many bacterial pathogens for the delivery of effector molecules that subvert varied host cellular processes. brucella spp. are facultative intracellular pathogens capable of survival and replication inside mammalian cells. brucella t4ss (virb) is essential to subvert lysosome fusion and to create an organelle permissive for replication. one possible role for virb is to translocate effector proteins that modulate host cell ... | 2011 | 21707904 |
| emerging agents to combat complicated and resistant infections: focus on ceftobiprole. | antimicrobial resistance is a global concern. over the past few years, considerable efforts and resources have been expended to detect, monitor, and understand at the basic level the many different facets of emerging and increasing resistance. development of new antimicrobial agents has been matched by the development of new mechanisms of resistance by bacteria. current antibiotics act at a variety of sites within the target bacteria, including the cross-linking enzymes in the cell wall, various ... | 2010 | 21694889 |
| Microbial diversity in the midguts of field and lab-reared populations of the European corn borer Ostrinia nubilalis. | BACKGROUND: Insects are associated with microorganisms that contribute to the digestion and processing of nutrients. The European Corn Borer (ECB) is a moth present world-wide, causing severe economical damage as a pest on corn and other crops. In the present work, we give a detailed view of the complexity of the microorganisms forming the ECB midgut microbiota with the objective of comparing the biodiversity of the midgut-associated microbiota and explore their potential as a source of genes an ... | 2011 | 21738787 |
| Recurrent Ochrobactrum anthropi and Shewanella putrefaciens bloodstream infection complicating hemodialysis. | Bloodstream infections (BSIs) are common in hemodialysis, especially when the access is a catheter. These infections are more commonly gram-positive bacteria or gram-negative bacilli and on some occasions, fungi. Ochrobactrum anthropi and Shewanella putrefaciens are ubiquitous hydrophilic gram-negative bacilli. There have been three cases of O.?anthropi BSI reported in hemodialysis patients (one from the United States and two from Vienna) and two cases of S.?putrefaciens BSI in hemodialysis pati ... | 2011 | 22099546 |
| effect of glucose on the fatty acid composition of cupriavidus necator jmp134 during 2,4-dichlorophenoxyacetic acid degradation: implications for lipid-based stable isotope probing methods. | combining lipid biomarker profiling with stable isotope probing (sip) is a powerful technique for studying specific microbial populations responsible for the degradation of organic pollutants in various natural environments. however, the presence of other easily degradable substrates may induce significant physiological changes by altering both the rate of incorporation of the target compound into the biomass and the microbial lipid profiles. in order to test this hypothesis, cupriavidus necator ... | 2011 | 21856833 |
| molecular determinants for substrate selectivity of ω-transaminases. | ω-transaminase (ω-ta) is an industrially important enzyme for production of chiral amines. about 20 (s)-specific ω-tas known to date show remarkably similar substrate selectivity characterized by stringent steric constraint precluding entry of a substituent larger than an ethyl group in the small binding pocket (s) and dual recognition of an aromatic substituent as well as a carboxylate group in the large pocket (l). the strictly defined substrate selectivity of the available ω-tas remains a lim ... | 2011 | 21983703 |
| isolation and characterization of ochrobactrum anthropi and ochrobactrum pecoris from caecal content of commercial turkeys. | two gram negative, micro-aerophilic, non-motile and non-spore-forming coccoid bacteria were isolated from female turkey caecal samples collected from a slaughterhouse. the biochemical reaction profiles (api 20 e and api 20 ne) typed both strains as ochrobactrum anthropi. on the basis of 16s rrna gene and reca gene sequence similarities the strains were identified as o. anthropi and ochrobactrum pecoris, respectively. both strains were highly resistant against beta-lactam antibiotics, chloramphen ... | 2011 | 21955448 |
| environmental microbiota represents a natural reservoir for dissemination of clinically relevant metallo-beta-lactamases. | a total of 10 metallo-β-lactamase-producing isolates of six different species, including brevundimonas diminuta (n = 3), rhizobium radiobacter (n = 2), pseudomonas monteilii (n = 1), pseudomonas aeruginosa (n = 2), ochrobactrum anthropi (n = 1), and enterobacter ludwigii (n = 1), were detected in the sewage water of a hospital. the presence of bla(vim-13) associated with a tn1721-class 1 integron structure was detected in all but one of the isolates (e. ludwigii, which produced vim-2), and in tw ... | 2011 | 21859934 |
| the three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis. | we performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (nylc) from agromyces sp. at 2.0 å-resolution. this enzyme is a member of the n-terminal nucleophile (n-tn) hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. we observed four identical heterodimers (27kda+9kda), which resulted from the autoprocessing of the precursor protein (36kda) and which constitute the doughnut-shaped quaternary structure. the catalytic resi ... | 2011 | 22187439 |
| the three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis. | we performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (nylc) from agromyces sp. at 2.0 å-resolution. this enzyme is a member of the n-terminal nucleophile (n-tn) hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. we observed four identical heterodimers (27kda+9kda), which resulted from the autoprocessing of the precursor protein (36kda) and which constitute the doughnut-shaped quaternary structure. the catalytic resi ... | 2011 | 22187439 |
| transcriptional analysis of a photorhabdus sp. variant reveals transcriptional control of phenotypic variation and multifactorial pathogenicity in insects. | photorhabdus luminescens lives in a mutualistic association with entomopathogenic nematodes and is pathogenic for insects. variants of photorhabdus frequently arise irreversibly and are studied because they have altered phenotypic traits that are potentially important for the host interaction. var* is a colonial and phenotypic variant displaying delayed pathogenicity when directly injected into the insect, spodoptera littoralis. in this study, we evaluated the role of transcriptomic modulation i ... | 2010 | 21131515 |
| antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria). | the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ... | 2011 | 22095134 |
| enzymatic properties of the glycine d-alanine aminopeptidase of aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji). | the gdaa gene encoding s12 family glycine-d: -alanine aminopeptidase (gdaa) was found in the industrial fungus aspergillus oryzae. gdaa shares 43% amino acid sequence identity with the d: -aminopeptidase of the gram-negative bacterium ochrobactrum anthropi. gdaa purified from an a. oryzae gdaa-overexpressing strain exhibited high d: -stereospecificity and efficiently released n-terminal glycine and d: -alanine of substrates in a highly specific manner. the optimum ph and temperature were 8 to 9 ... | 2011 | 22005737 |
| transcriptional analysis of a photorhabdus sp. variant reveals transcriptional control of phenotypic variation and multifactorial pathogenicity in insects. | photorhabdus luminescens lives in a mutualistic association with entomopathogenic nematodes and is pathogenic for insects. variants of photorhabdus frequently arise irreversibly and are studied because they have altered phenotypic traits that are potentially important for the host interaction. var* is a colonial and phenotypic variant displaying delayed pathogenicity when directly injected into the insect, spodoptera littoralis. in this study, we evaluated the role of transcriptomic modulation i ... | 2010 | 21131515 |
| the rhizome of reclinomonas americana, homo sapiens, pediculus humanus and saccharomyces cerevisiae mitochondria. | mitochondria are thought to have evolved from eubacteria-like endosymbionts; however, the origin of the mitochondrion remains a subject of debate. in this study, we investigated the phenomenon of chimerism in mitochondria to shed light on the origin of these organelles by determining which species played a role in their formation. we used the mitochondria of four distinct organisms, reclinomonas americana, homo sapiens, saccharomyces cerevisiae and multichromosome pediculus humanus, and attempte ... | 2011 | 22014084 |
| identification of potential diagnostic markers among burkholderia cenocepacia and b. multivorans supernatants. | patients with cystic fibrosis (cf) are susceptible to chronic respiratory infections with a number of bacterial pathogens. among them, the burkholderia cepacia complex (bcc) bacteria, consisting of nine related species, have emerged as problematic cf pathogens due to their antibiotic resistance, incidence of nosocomial infection, and person-to-person transmission. bcc organisms present the clinical microbiologist with a diagnostic dilemma due to the lack of phenotypic biochemical or growth-relat ... | 2010 | 20810766 |
| first comprehensive evaluation of the m.i.c. evaluator device for aerobic gram-positive and gram-negative bacterial species compared to etest and clsi broth micro-dilution. | the m.i.c.evaluator™ strip (thermo fisher scientific, basingstoke, uk) uses similar methodology to etest. in this first assessment of the m.i.c.evaluator device, 409 strains of aerobic gram-positive bacteria (staphylococci, streptococci and enterococci) and 325 strains of enterobacteriaceae, pseudomonas species and acinetobacter species, were tested by m.i.c.evaluator strip, etest and by broth micro-dilution as a reference standard. the gram-positives included staphylococci (mrsa, mssa and cns), ... | 2012 | 22238441 |
| 1,3-propanediol production potential of clostridium saccharobutylicum nrrl b-643. | owing to the significant interest in biofuel production in the form of biodiesel, vast amount of glycerol as a waste product is produced all over the world. among the economically viable and ecologically acceptable solutions for the safe disposal of this waste, biotechnological conversion of glycerol into a valuable bioplastic raw material, namely 1,3-propanediol (1,3-pdo) seems to be very promising. in this study, 1,3-pdo production potential of clostridium saccharobutylicum nrrl b-643 was stud ... | 2010 | 20647065 |
| isolation from ochrobactrum anthropi of a novel class ii 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate. | applying the genomic library construction process and colony screening, a novel aroa gene encoding 5-enopyruvylshikimate-3-phosphate synthase from ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance. | 2010 | 20601515 |
| a universal method for the identification of bacteria based on general pcr primers. | the universal method (um) described here will allow the detection of any bacterial rdna leading to the identification of that bacterium. the method should allow prompt and accurate identification of bacteria. the principle of the method is simple; when a pure pcr product of the 16s gene is obtained, sequenced, and aligned against bacterial dna data base, then the bacterium can be identified. confirmation of identity may follow. in this work, several general 16s primers were designed, mixed and a ... | 2011 | 23024404 |
| perspective for aquaponic systems: "omic" technologies for microbial community analysis. | aquaponics is the combined production of aquaculture and hydroponics, connected by a water recirculation system. in this productive system, the microbial community is responsible for carrying out the nutrient dynamics between the components. the nutrimental transformations mainly consist in the transformation of chemical species from toxic compounds into available nutrients. in this particular field, the microbial research, the "omic" technologies will allow a broader scope of studies about a cu ... | 2015 | 26509157 |
| annotation of protein domains reveals remarkable conservation in the functional make up of proteomes across superkingdoms. | the functional repertoire of a cell is largely embodied in its proteome, the collection of proteins encoded in the genome of an organism. the molecular functions of proteins are the direct consequence of their structure and structure can be inferred from sequence using hidden markov models of structural recognition. here we analyze the functional annotation of protein domain structures in almost a thousand sequenced genomes, exploring the functional and structural diversity of proteomes. we find ... | 2011 | 24710297 |
| highly diverse nirk genes comprise two major clades that harbour ammonium-producing denitrifiers. | copper dependent nitrite reductase, nirk, catalyses the key step in denitrification, i.e. nitrite reduction to nitric oxide. distinct structural nirk classes and phylogenetic clades of nirk-type denitrifiers have previously been observed based on a limited set of nirk sequences, however, their environmental distribution or ecological strategies are currently unknown. in addition, environmental nirk-type denitrifiers are currently underestimated in pcr-dependent surveys due to primer coverage lim ... | 2016 | 26923558 |
| integron diversity in heavy-metal-contaminated mine tailings and inferences about integron evolution. | integrons are horizontal gene transfer (hgt) systems containing elements necessary for site-specific recombination and expression of foreign dna. the overall phylogenetic distribution of integrons and range of genes that can be transferred by integrons are unknown. this report contains an exploration of integrons in an environmental microbial community and an investigation of integron evolution. first, using culture-independent techniques, we explored the diversity of integrons and integron-tran ... | 2004 | 14766601 |
| comparative molecular analysis of chemolithoautotrophic bacterial diversity and community structure from coastal saline soils, gujarat, india. | soils harbour high diversity of obligate as well as facultative chemolithoautotrophic bacteria that contribute significantly to co2 dynamics in soil. in this study, we used culture dependent and independent methods to assess the community structure and diversity of chemolithoautotrophs in agricultural and coastal barren saline soils (low and high salinity). we studied the composition and distribution of chemolithoautotrophs by means of functional marker gene cbbl encoding large subunit of ribulo ... | 2012 | 22834535 |
| a consortium of aggregatibacter actinomycetemcomitans, streptococcus parasanguinis, and filifactor alocis is present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis. | aggregatibacter actinomycetemcomitans-induced localized aggressive periodontitis (lap) in african-american adolescents has been documented but is poorly understood. two thousand fifty-eight adolescents aged 11 to 17 years were screened for their periodontal status and the presence of a. actinomycetemcomitans in their oral cavity. seventy-one a. actinomycetemcomitans-negative and 63 a. actinomycetemcomitans-positive periodontally healthy subjects were enrolled, sampled, examined, and radiographed ... | 2013 | 23784124 |
| structure and immunogenicity of the rough-type lipopolysaccharide from the periodontal pathogen tannerella forsythia. | tannerella forsythia is a gram-negative anaerobic organism that inhabits subgingival plaque biofilms and is covered with a so far unique surface layer composed of two glycoproteins. it belongs to the so-called "red complex" of bacteria comprising species that are associated with periodontal disease. while the surface layer glycoprotein glycan structure had been elucidated recently and found to be a virulence factor, no structural data on the lipopolysaccharide (lps) of this organism were availab ... | 2013 | 23616409 |
| exploiting quorum sensing to confuse bacterial pathogens. | cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. with the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. an antivirulence approach by which quorum sensing is impeded has caught on as ... | 2013 | 23471618 |
| the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members. | the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members. | 2016 | 27448413 |
| extensive identification of bacterial riboflavin transporters and their distribution across bacterial species. | riboflavin, the precursor for the cofactors flavin mononucleotide (fmn) and flavin adenine dinucleotide, is an essential metabolite in all organisms. while the functions for de novo riboflavin biosynthesis and riboflavin import may coexist in bacteria, the extent of this co-occurrence is undetermined. the ribm, ribn, rfuabcd and the energy-coupling factor-ribu bacterial riboflavin transporters have been experimentally characterized. in addition, impx, rfnt and ribxy are proposed as riboflavin tr ... | 2015 | 25938806 |
| direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. | with long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (maldi-tof) mass spectrometry was demonstrated to accurately identify bacteria routinely isolated in a clinical biology laboratory. in order to speed up the identification proce ... | 2009 | 19946369 |
| functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter. | targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ... | 2013 | 24062736 |
| a gene transfer agent and a dynamic repertoire of secretion systems hold the keys to the explosive radiation of the emerging pathogen bartonella. | gene transfer agents (gtas) randomly transfer short fragments of a bacterial genome. a novel putative gta was recently discovered in the mouse-infecting bacterium bartonella grahamii. although gtas are widespread in phylogenetically diverse bacteria, their role in evolution is largely unknown. here, we present a comparative analysis of 16 bartonella genomes ranging from 1.4 to 2.6 mb in size, including six novel genomes from bartonella isolated from a cow, two moose, two dogs, and a kangaroo. a ... | 2013 | 23555299 |
| a persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations. | acute exacerbations of chronic obstructive pulmonary disease (copd) are a major source of morbidity and contribute significantly to healthcare costs. although bacterial infections are implicated in nearly 50% of exacerbations, only a handful of pathogens have been consistently identified in copd airways, primarily by culture-based methods, and the bacterial microbiota in acute exacerbations remains largely uncharacterized. the aim of this study was to comprehensively profile airway bacterial com ... | 2010 | 20141328 |
| distribution of genes encoding nucleoid-associated protein homologs in plasmids. | bacterial nucleoid-associated proteins (naps) form nucleoprotein complexes and influence the expression of genes. recent studies have shown that some plasmids carry genes encoding nap homologs, which play important roles in transcriptional regulation networks between plasmids and host chromosomes. in this study, we determined the distributions of the well-known naps fis, h-ns, hu, ihf, and lrp and the newly found naps mvat and ndpa among the whole-sequenced 1382 plasmids found in gram-negative b ... | 2011 | 21350637 |
| across bacterial phyla, distantly-related genomes with similar genomic gc content have similar patterns of amino acid usage. | the gc content of bacterial genomes ranges from 16% to 75% and wide ranges of genomic gc content are observed within many bacterial phyla, including both gram negative and gram positive phyla. thus, divergent genomic gc content has evolved repeatedly in widely separated bacterial taxa. since genomic gc content influences codon usage, we examined codon usage patterns and predicted protein amino acid content as a function of genomic gc content within eight different phyla or classes of bacteria. w ... | 2011 | 21423704 |
| nitric oxide reductase (norb) genes from pure cultures and environmental samples. | a pcr-based approach was developed to recover nitric oxide (no) reductase (norb) genes as a functional marker gene for denitrifying bacteria. norb database sequences grouped in two very distinct branches. one encodes the quinol-oxidizing single-subunit class (qnorb), while the other class is a cytochrome bc-type complex (cnorb). the latter oxidizes cytochrome c, and the gene is localized adjacent to norc. while both norb types occur in denitrifying strains, the qnorb type was also found in a var ... | 2003 | 12788753 |
| improved assessment of denitrifying, n2-fixing, and total-community bacteria by terminal restriction fragment length polymorphism analysis using multiple restriction enzymes. | a database of terminal restriction fragments (trfs) of the 16s rrna gene was set up utilizing 13 restriction enzymes and 17,327 genbank sequences. a computer program, termed trefid, was developed to allow identification of any of these 17,327 sequences by means of polygons generated from the specific trfs of each bacterium. the trefid program complements and exceeds in its data content the web-based phylogenetic assignment tool recently described by a. d. kent, d. j. smith, b. j. benson, and e. ... | 2005 | 15812035 |
| microarray-based analysis of subnanogram quantities of microbial community dnas by using whole-community genome amplification. | microarray technology provides the opportunity to identify thousands of microbial genes or populations simultaneously, but low microbial biomass often prevents application of this technology to many natural microbial communities. we developed a whole-community genome amplification-assisted microarray detection approach based on multiple displacement amplification. the representativeness of amplification was evaluated using several types of microarrays and quantitative indexes. representative det ... | 2006 | 16820490 |
| higher diversity and abundance of denitrifying microorganisms in environments than considered previously. | denitrification is an important process in the global nitrogen cycle. the genes encoding nirk and nirs (nirk and nirs), which catalyze the reduction of nitrite to nitric oxide, have been used as marker genes to study the ecological behavior of denitrifiers in environments. however, conventional polymerase chain reaction (pcr) primers can only detect a limited range of the phylogenetically diverse nirk and nirs. thus, we developed new pcr primers covering the diverse nirk and nirs. clone library ... | 2015 | 25756678 |
| beneficial cyanobacteria and eubacteria synergistically enhance bioavailability of soil nutrients and yield of okra. | microorganisms in the rhizosphere mediate the cycling of nutrients, their enhanced mobilisation and facilitate their uptake, leading to increased root growth, biomass and yield of plants. we examined the promise of beneficial cyanobacteria and eubacteria as microbial inoculants, applied singly or in combination as consortia or biofilms, to improve growth and yields of okra. interrelationships among the microbial activities and the micro/macro nutrient dynamics in soils and okra yield characteris ... | 2016 | 27441245 |
| role of ser11 in the stabilization of the structure of ochrobactrum anthropi glutathione transferase. | gsts (glutathione transferases) are a multifunctional group of enzymes, widely distributed and involved in cellular detoxification processes. in the xenobiotic-degrading bacterium ochrobactrum anthropi, gst is overexpressed in the presence of toxic concentrations of aromatic compounds such as 4-chlorophenol and atrazine. we have determined the crystal structure of the gst from o. anthropi (oagst) in complex with gsh. like other bacterial gsts, oagst belongs to the beta class and shows a similar ... | 2007 | 17223798 |
| [isolation and characterization of a sodium dodecyl benzene sulfonate degrading bacterial strain]. | a bacterial strain, designated as wzr-a, which could utilize sodium dodecyl benzene sulfonate (sdbs) as sole carbon and energy source for growth, was isolated from contaminated river. the strain was identified as ochrobactrum anthropi based on its morphological and physiological properties, and 16s rdna sequence analysis. the optimum ph and temperature for cell growth and sdbs degradation were 7.0 and 30 degrees u, respectively. the degradation rate of sdbs by strain wzr-a was higher than 80% wh ... | 2006 | 17302167 |
| enigmatic dual symbiosis in the excretory organ of nautilus macromphalus (cephalopoda: nautiloidea). | symbiosis is an important driving force in metazoan evolution and the study of ancient lineages can provide an insight into the influence of symbiotic associations on morphological and physiological adaptations. in the 'living fossil' nautilus, bacterial associations are found in the highly specialized pericardial appendage. this organ is responsible for most of the excretory processes (ultrafiltration, reabsorption and secretion) and secretes an acidic ammonia-rich excretory fluid. in this stud ... | 2007 | 17311780 |
| crystal structure and functional characterization of a d-stereospecific amino acid amidase from ochrobactrum anthropi sv3, a new member of the penicillin-recognizing proteins. | d-amino acid amidase (daa) from ochrobactrum anthropi sv3, which catalyzes the stereospecific hydrolysis of d-amino acid amides to yield the d-amino acid and ammonia, has attracted increasing attention as a catalyst for the stereospecific production of d-amino acids. in order to clarify the structure-function relationships of daa, the crystal structures of native daa, and of the d-phenylalanine/daa complex, were determined at 2.1 and at 2.4 a resolution, respectively. both crystals contain six s ... | 2007 | 17331533 |
| ochrobactrum cytisi sp. nov., isolated from nodules of cytisus scoparius in spain. | two strains named esc1(t) and esc5 were isolated from nodules of cytisus scoparius growing in a spanish soil. phylogenetic analysis of the 16s rrna gene showed that these strains belong to the genus ochrobactrum, their closest relatives being ochrobactrum anthropi and ochrobactrum lupini, with 100 and 99.9 % similarity to the respective type strains. despite this high similarity, the results of dna-dna hybridization, phenotypic tests and fatty acid analyses showed that these strains represent a ... | 2007 | 17392207 |
| spontaneous bacterial peritonitis due to ochrobactrum anthropi: a case report. | we report a case of spontaneous bacterial peritonitis from ochrobactrum anthropi. o. anthropi is recognized as an emerging pathogen in immunocompromised patients. in contrast to most previously described cases, the patient reported here had no indwelling catheter. to our knowledge, no case of o. anthropi spontaneous bacterial peritonitis has been reported in the medical literature until now. | 2007 | 17449955 |
| enzyme-catalyzed formation of beta-peptides: beta-peptidyl aminopeptidases bapa and dmpa acting as beta-peptide-synthesizing enzymes. | in recent studies, we discovered that the three beta-peptidyl aminopeptidases, bapa from sphingosinicella xenopeptidilytica 3-2w4, bapa from s. microcystinivorans y2, and dmpa from ochrobactrum anthropi lmg7991, possess the unique feature of cleaving n-terminal beta-amino acid residues from beta- and alpha/beta-peptides. herein, we investigated the use of the same three enzymes for the reverse reaction catalyzing the oligomerization of beta-amino acids and the synthesis of mixed peptides with n- ... | 2007 | 17886858 |
| ochrobactrum anthropi bacteremia in a child with inborn error of mitochondrial fatty acid oxidation. | objective: to report an incident of bacteremia caused by ochrobactrum anthropi. case presentation and intervention: the case of a female child aged 2 years and 10 months with a known history of long-chain 3-hydroxyacyl-coenzyme a dehydrogenase deficiency who developed o. anthropi bacteremia during hospital stay is presented. patient's history, clinical findings, laboratory and radiological investigations were thoroughly reviewed. the cultured organism was identified using microscan walkaway 96 s ... | 2007 | 17917448 |
| [ochrobactrum anthropi bacteremia developed after cholangiopancreatography]. | ochrobactrum anthropi (formerly achromobacter spp.) is an aerobic, motile, oxidase positive and lactose negative gram negative bacillus which is widely distributed in the environment and water sources. in recent publications, o. anthropi has an increasing importance as a nosocomial infection agent. the aim of this report was to present a case of o. anthropi bacteremia developed after endoscopic retrograde cholangiopancreatography (ercp). a 89-year old female patient presented with high fever one ... | 2007 | 17933261 |
| structures of d-amino-acid amidase complexed with l-phenylalanine and with l-phenylalanine amide: insight into the d-stereospecificity of d-amino-acid amidase from ochrobactrum anthropi sv3. | the crystal structures of d-amino-acid amidase (daa) from ochrobactrum anthropi sv3 in complex with l-phenylalanine and with l-phenylalanine amide were determined at 2.3 and 2.2 a resolution, respectively. comparison of the l-phenylalanine amide complex with the d-phenylalanine complex reveals that the d-stereospecificity of daa might be achieved as a consequence of three structural factors: (i) the hydrophobic cavity in the region in which the hydrophobic side chain of the substrate is held, (i ... | 2008 | 18323628 |
| chronic postoperative roseomonas endophthalmitis. | we report one case with chronic postoperative endophthalmitis caused by roseomonas species. roseomonas spp. induced chronic endophthalmitis, which might result in misdiagnosis and delayed treatment and causes ocular damage and severe visual loss. this report is the first one related to a case with postoperative endophthalmitis secondary to roseomonas infection. | 2009 | 18987177 |
| chronic postoperative roseomonas endophthalmitis. | we report one case with chronic postoperative endophthalmitis caused by roseomonas species. roseomonas spp. induced chronic endophthalmitis, which might result in misdiagnosis and delayed treatment and causes ocular damage and severe visual loss. this report is the first one related to a case with postoperative endophthalmitis secondary to roseomonas infection. | 2009 | 18987177 |
| run-off replication of host-adaptability genes is associated with gene transfer agents in the genome of mouse-infecting bartonella grahamii. | the genus bartonella comprises facultative intracellular bacteria adapted to mammals, including previously recognized and emerging human pathogens. we report the 2,341,328 bp genome sequence of bartonella grahamii, one of the most prevalent bartonella species in wild rodents. comparative genomics revealed that rodent-associated bartonella species have higher copy numbers of genes for putative host-adaptability factors than the related human-specific pathogens. many of these gene clusters are loc ... | 2009 | 19578403 |