| detergent inhibition of nitric-oxide reductase activity. | gas chromatography revealed that exposure of extracts of the denitrifiers 'achromobacter cycloclastes', paracoccus denitrificans, pseudomonas aeruginosa and pseudomonas perfectomarina to triton x-100 inhibited reduction of no to n2o, and thus concomitantly inhibited reduction of no2- to n2o. after exposure of extracts to triton x-100, the ratio of h+ consumed to no2- added decreased from approx. 2.0 (for untreated extracts) to approx. 1.5, which indicated that no2- was reduced to no by the treat ... | 1987 | 3028488 |
| a simple, one-step purification of cytochrome b from the bc1 complexes of bacteria. | | 1987 | 3030803 |
| laser flash photolysis studies of electron transfer between ferredoxin-nadp+ reductase and several high-potential redox proteins. | complex formation and the kinetics of electron transfer between ferredoxin-nadp+ reductase (fnr) and two structurally homologous acidic 4fe-4s high-potential ferredoxins (hipip's) from ectothiorhodospira halophila (hp1 and hp2) and two structurally homologous cytochromes c2 from paracoccus denitrificans and rhodospirillum rubrum (pc2, and rc2, respectively) have been investigated by gel filtration and laser flash photolysis techniques. gel filtration studies indicated that complex formation occu ... | 1987 | 3032236 |
| purification and properties of methylamine dehydrogenase from paracoccus denitrificans. | methylamine dehydrogenase from paracoccus denitrificans was purified to homogeneity in two steps from the periplasmic fraction of methylamine-grown cells. the enzyme exhibited a pi value of 4.3 and was composed of two 46,700-dalton subunits and two 15,500-dalton subunits. each small subunit possessed a covalently bound pyrrolo-quinoline quinone prosthetic group. the amino acid compositions of the large and small subunits are very similar to those of other methylamine dehydrogenases which have be ... | 1987 | 3558322 |
| distribution and types of bacteria isolated from an inland saltern. | the physico-chemical characteristics and bacterial populations of an inland saltern were studied. the well water leading into the ponds of the saltern differed from sea water in its total salinity (18% (w/v)) and had lower mg2+ and so4(2-) contents and much higher contents of cl-, ca2+- and k+. a high proportion of halotolerant microorganisms were isolated, although marine bacteria were not found. most of the halophilic strains isolated appeared to be typical moderate halophiles, and included si ... | 1987 | 3606874 |
| purification and some characteristics of nitrous oxide reductase from paracoccus denitrificans. | nitrous oxide reductase from the denitrifying bacterium paracoccus denitrificans has been purified very nearly to homogeneity by an anaerobic procedure that results in a product with high specific activity. the enzyme is a dimer of about mr 144,000 composed of two subunits of apparently equal mr and contains 4 mol of cu per mol of subunit. the isoelectric point is 4.3; specific activity at 25 degrees c, ph 7.1, is 122 mumol x min-1 x mg of protein-1; and km is about 7 microm n2o under the same c ... | 1987 | 3032972 |
| subfractionation and characterization of soluble c-type cytochromes from paracoccus denitrificans cultured under various limiting conditions in the chemostat. | soluble c-type cytochromes were partially purified from paracoccus denitrificans cells grown in succinate- and methanol-limited aerobic, nitrate-limited anaerobic and oxygen-limited chemostat cultures. five c types could be distinguished with the following apparent molecular masses, absorption maxima and midpoint potentials. (a) 9.2 kda, 549 nm and +190 mv; (b) 14 kda, 549 nm and +227 mv; (c) 22 kda, 552 nm and +190 mv; (d) 30 kda, 552.7 nm and +160 mv; (e) 45 kda, a dihaem: 555 nm, +128 mv and ... | 1987 | 3036513 |
| identification of a stable ubisemiquinone and characterization of the effects of ubiquinone oxidation-reduction status on the rieske iron-sulfur protein in the three-subunit ubiquinol-cytochrome c oxidoreductase complex of paracoccus denitrificans. | the ubiquinol-cytochrome c oxidoreductase (cytochrome bc1) complex from paracoccus denitrificans exhibits a thermodynamically stable ubisemiquinone radical detectable by epr spectroscopy. the radical is centered at g = 2.004, is sensitive to antimycin, and has a midpoint potential at ph 8.5 of +42 mv. these properties are very similar to those of the stable ubisemiquinone (qi) previously characterized in the cytochrome bc1 complexes of mitochondria. the micro-environment of the rieske iron-sulfu ... | 1987 | 3036822 |
| epr characterization of the iron-sulfur clusters in the nadh: ubiquinone oxidoreductase segment of the respiratory chain in paracoccus denitrificans. | the physicochemical properties of the iron-sulfur clusters present in the nadh:ubiquinone oxidoreductase of paracoccus denitrificans have been examined in the cytoplasmic membrane particles by redox potentiometry and epr spectroscopy. analogous to the iron-sulfur clusters present in the mitochondrial nadh: ubiquinone oxidoreductase, we have found two binuclear and three tetranuclear epr detectable iron-sulfur clusters, namely, n-1a, n-1b, n-2, n-3, and n-4. in the bacterial system, the two binuc ... | 1987 | 3036849 |
| interposon mutagenesis of soil and water bacteria: a family of dna fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. | we have constructed a series of derivatives of the omega interposon [prentki and krisch, gene 29 (1984) 303-313] that can be used for in vitro insertional mutagenesis. each of these dna fragments carries a different antibiotic or hg2+ resistance gene (apr, cmr, tcr, kmr or hgr) which is flanked, in inverted orientation, by transcription and translation termination signals and by synthetic polylinkers. the dna of these interposons can be easily purified and then inserted, by in vitro ligation, in ... | 1987 | 3038679 |
| complete amino acid sequence of cytochrome c551 from erythrobacter species strain och 114. | the complete amino acid sequence of cytochrome c551 isolated from an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, was determined. the cytochrome molecule was composed of a total of 119 amino acid residues and its molecular weight including heme was calculated to be 13,235. the sequence was (sequence: see text). its molecular weight indicates that this cytochrome is of the l-type. sequence alignment with other bacterial cytochromes c shows that this cytochrome is similar to ... | 1987 | 3038859 |
| physiological regulation of paracoccus denitrificans methanol dehydrogenase synthesis and activity. | an enzyme-linked immunosorbent assay and a whole-cell activity assay were developed which allowed detection of methanol dehydrogenase (mdh) of paracoccus denitrificans with increased sensitivity. by these methods, it was shown that mdh was not induced by its natural substrate, methanol. relief from a catabolite repression-like mechanism seemed responsible for low-level mdh synthesis, while product induction was the hypothesized mechanism for synthesis of high amounts of mdh. in the latter proces ... | 1988 | 3042759 |
| different lipid a types in lipopolysaccharides of phototrophic and related non-phototrophic bacteria. | lipid a analyses confirm not only the present taxa of the purple nonsulfur bacteria (formerly rhodospirillaceae), but also phylogenetical relatedness of distinct phototrophic to distinct non-phototrophic bacteria, as was suggested by cataloguing 16s rrna. for example, lipid a with ester-bound 3-oh-10:0 and the rare amide-linked 3-oxo-14:0 is common to the phototrophic rhodobacter capsulatus and rhodobacter sphaeroides and also to paracoccus denitrificans and thiobacillus versutus. 'lipid adag' ( ... | 1988 | 3078741 |
| genetics of hydrogenase from aerobic lithoautotrophic bacteria. | aerobic facultatively autotrophic hydrogen bacteria are distinguished on the basis of their hydrogen-oxidizing enzyme system (hox). the major group, represented by paracoccus denitrificans and pseudomonas facilis, contains a membrane-bound, electron transport-coupled protein. species of nocardia are characterized by the possession of a cytoplasmic nad-dependent hydrogenase. both enzymes are present in strains of alcaligenes. all hydrogenases from lithoautotrophs are h2-consuming nickel-iron-sulf ... | 1986 | 3089306 |
| purification and characterization of nadh dehydrogenase complex from paracoccus denitrificans. | an nadh dehydrogenase complex was isolated from the plasma membranes of aerobically grown paracoccus denitrificans cells by extraction with nabr and purification on an nad-agarose column. the nadh-ubiquinone-1 reductase activity of the isolated nadh dehydrogenase complex was about 10 times higher than that of the nabr extract. the preparation was composed of 10 (6 major and 4 minor) unlike polypeptides, and lacked identifiable components and activities characteristic of other enzyme complexes of ... | 1986 | 3096211 |
| inhibition of nadh-ubiquinone reductase activity by n,n'-dicyclohexylcarbodiimide and correlation of this inhibition with the occurrence of energy-coupling site 1 in various organisms. | the nadh-ubiquinone reductase activity of the respiratory chains of several organisms was inhibited by the carboxyl-modifying reagent n,n'-dicyclohexylcarbodiimide (dccd). this inhibition correlated with the presence of an energy-transducing site in this segment of the respiratory chain. where the nadh-quinone reductase segment involved an energy-coupling site (e.g., in bovine heart and rat liver mitochondria, and in paracoccus denitrificans, escherichia coli, and thermus thermophilus hb-8 membr ... | 1987 | 3111526 |
| nitric oxide-dependent proton translocation in various denitrifiers. | respiration of no resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. paracoccus denitrificans, rhodopseudomonas sphaeroides subsp. denitrificans, "achromobacter cycloclastes," and rhizobium japonicum gave, respectively, h+/no ratios of 3.65, 4.96, 1.94, and 1.12. antimycin a completely inhibited no-dependent proton translocation in p. denitrificans and severely restricted translocation in the r. sphaeroides strain. proton uptake ... | 1985 | 3928599 |
| the respiratory chain and energy conservation in the mitochondrion-like bacterium paracoccus denitrificans. | paracoccus denitrificans has a flexible electron transport chain containing various inducible dehydrogenases, oxidases and reductases. the sequence of electron carriers and stoichiometry of energy transduction is well known, with the exception of all functional aspects of the different c-type cytochromes. the mitochondrion-like characteristics of the respiratory chain have stimulated much research at the molecular level, which should help to elucidate the structure-function relationship of the c ... | 1987 | 3153596 |
| complex formation between methylamine dehydrogenase and amicyanin from paracoccus denitrificans. | two proteins isolated from paracoccus denitrificans, the copper-containing electron carrier amicyanin and the pyrroloquinoline quinone-containing enzyme methylamine dehydrogenase, have been shown to form a complex. complex formation between methylamine dehydrogenase and either oxidized or reduced amicyanin resulted in alterations in the absorbance spectrum of the pyrroloquinoline quinone prosthetic group of methylamine dehydrogenase. binding of amicyanin to the enzyme exhibited positive cooperat ... | 1988 | 3170535 |
| statistical analysis of growth limitations in paracoccus denitrificans: an experiment with a completely randomized two-way factorial design with replications. | cultivation of microorganisms under growth limitation is a widely used technique in microbiology. the relevant investigations, though seemingly based on straightforward experiments, have generated conflicting results, e.g., concerning molar growth yields. the purpose of this paper is to show that discrepancies in the literature could be solved if more attention were paid to methodology, especially statistics. new experimental results, concerning growth limitations in paracoccus denitrificans, wi ... | 1985 | 4004200 |
| preliminary x-ray crystallographic studies of methylamine dehydrogenase and methylamine dehydrogenase--amicyanin complexes from paracoccus denitrificans. | | 1988 | 3210240 |
| a bacteriophage t4 expression cassette that functions efficiently in a wide range of gram-negative bacteria. | we have constructed a derivative of the broad-host-range vector rsf1010. this plasmid, p alpha omega, contains an expression cassette derived from bacteriophage t4 gene 32, into which we have inserted the coding sequence for the xyle enzyme (c2,3o) of the tol plasmid pwwo. the composite plasmid, p alpha xyle omega, was transferred by conjugal mobilisation into a variety of gram-negative bacteria (agrobacter, paracoccus, erwinia, pseudomonas, rhizobium and xanthomonas). high levels of c2,3o activ ... | 1988 | 3259198 |
| trapping of nitric oxide produced during denitrification by extracellular hemoglobin. | a spectrophotometric method has been developed that uses extracellular hemoglobin (hb) to trap nitric oxide (no) released during denitrification as nitrosyl hemoglobin (hbno). the rate of complexation of no with hb is about at the diffusion controlled limit for protein molecules and the product, hbno, is essentially stable. hb was added to an anaerobic bacterial suspension and denitrification was initiated with either kno2 or kno3. hbno formation was observed for six species of denitrifying bact ... | 1988 | 3339013 |
| [bacterial lipids]. | | 1974 | 4156687 |
| selective and reversible inhibition of the atpase of micrococcus denitrificans by 7-chloro-4-nitrobenzo-2-oxa-1,3 diazole. | | 1974 | 4278263 |
| the bacterial nitrate reductases: epr studies on nitrate reductase a from micrococcus denitrificans. | | 1972 | 4335844 |
| preliminary x-ray studies of a cytochrome c from micrococcus dentrificans. | | 1972 | 4346098 |
| recurrence of the cytochrome fold in a nitrate-respiring bacterium. | | 1973 | 4355597 |
| interaction of cytochrome oxidases aa3 and d with nitrite. | | 1973 | 4357109 |
| relationship between amino acid transport and electron transport by membrane vesicles of micrococcus denitrificans. | | 1974 | 4441098 |
| mechanisms of active transport in isolated bacterial membrane vesicles. 8. valinomycin-induced rubidium transport. | | 1973 | 4573982 |
| phospholipid composition and metabolism of micrococcus denitrificans. | the phospholipid composition of micrococcus denitrificans was unusual in that phosphatidyl choline (pc) was a major phospholipid (30.9%). other phospholipids were phosphatidyl glycerol (pg, 52.4%), phosphatidyl ethanolamine (pe, 5.8%), an unknown phospholipid (5.3%), cardiolipin (cl, 3.2%), phosphatidyl dimethylethanolamine (pdme, 0.9%), phosphatidyl monomethylethanolamine (pmme, 0.6%), phosphatidyl serine (ps, 0.5%), and phosphatidic acid (0.4%). kinetics of (32)p incorporation suggested that p ... | 1972 | 4640503 |
| structure of micrococcus denitrificans and m. halodenitrificans revealed by freeze-etching. | | 1973 | 4717955 |
| aminolaevulinate synthetase of micrococcus denitrificans. purification and properties of the enzyme, and the effect of growth conditions on the enzyme activity in cells. | 1. 5-aminolaevulinate synthetase was detected in extracts of the non-photosynthetic bacterium micrococcus denitrificans. 2. activity is high in cells grown anaerobically in a defined nitrate medium, but is low in cells grown in an iron-deficient medium, and in cells grown aerobically. 3. aminolaevulinate synthetase was purified extensively; it has a molecular weight of about 68000; apparent k(m) values for glycine, succinyl-coa and pyridoxal phosphate are 12mm, 10mum and 11mum respectively; 2mum ... | 1973 | 4722442 |
| control of aminolaevulinate synthetase in micrococcus denitrificans. | | 1973 | 4791044 |
| reduction and separation of nitrate and nitrite by liquid membrane-encapsulated enzymes. | | 1974 | 4854059 |
| branched electron-transport systems in bacteria. | | 1971 | 5005815 |
| acid-base titration across the plasma membrane of micrococcus denitrificans: factors affecting the effective proton conductance and the respiratory rate. | | 1970 | 5005951 |
| the influence of oxygen and nitrate on the formation of the cytochrome pigments of the aerobic and anaerobic respiratory chain of micrococcus denitrificans. | | 1972 | 5042842 |
| 5-aminolaevulinate synthetase of micrococcus denitrificans. | | 1972 | 5085583 |
| 19f-nuclear magnetic resonance: measurements of [o2] and ph in biological systems. | 19f-nuclear magnetic resonance (nmr) has been used to determine both intracellular ph and oxygen concentrations in cell suspensions. oxygen concentrations in paracoccus denitrificans and insulinoma cells, rinm5f, in the nmr probe can be monitored directly by 1/t1 measurements of perfluorotripropylamine (ftpa)/lecithin emulsion added to the suspensions. with ftpa oxygen monitoring, we investigated the relative aeration capabilities of two types of nmr chambers. both normal and transformed eucaryo ... | 1988 | 3345331 |
| respiratory nitrate reductase from paracoccus denitrificans. evidence for two b-type haems in the gamma subunit and properties of a water-soluble active enzyme containing alpha and beta subunits. | 1. the b-type haem centres of the three (alpha, beta and gamma) subunit nitrate reductase from paracoccus denitrificans have been analysed by redox potentiometry. two components were identified with mid-point potentials +95 mv and +210 mv. 2. washing, in the absence of mg2+ ions, of cytoplasmic membrane vesicles from p. denitrificans promoted selective release of nitrate reductase activity. the released enzyme was purified by chromatography and shown to contain alpha and beta, but not gamma poly ... | 1988 | 3371362 |
| increased outer membrane ornithine-containing lipid and lysozyme penetrability of paracoccus denitrificans grown in a complex medium deficient in divalent cations. | paracoccus denitrificans grown in a complex medium was highly susceptible to lysozyme, in contrast to cells grown in a complex medium supplemented with mg2+ and ca2+ or in a succinate-salts medium. the complex medium was deficient in divalent cations needed for optimum outer membrane stability. the major change in molecular compositions of outer membranes isolated from cells grown under the different conditions was a higher ratio of ornithine-containing lipid to phospholipid in complex-medium-gr ... | 1988 | 3384812 |
| stable isotope evidence for the localisation of phenylalanine biosynthesis in the bacterium paracoccus denitrificans. | paracoccus denitrificans was grown on [6-13c]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. the 13c-labelling pattern observed in phenylalanine indicated that the biosynthetic sequences of enzymes for phenylalanine production were unequally distributed within the cell and that there are at least 2 separate loci of phenylalanine biosynthesis. the principal locus of phenylalanine production was associated with ... | 1988 | 3390200 |
| demonstration of ferric l-parabactin-binding activity in the outer membrane of paracoccus denitrificans. | under low-iron conditions, paracoccus denitrificans excretes a catecholamine siderophore, l-parabactin, to sequester and utilize iron. in this report, we demonstrate the presence of stereospecific high-affinity ferric l-parabactin-binding activity associated with p. denitrificans membranes grown in low-iron medium. isolated outer membrane components were shown to be three to four times higher in specific activity for ferric l-parabactin. the same amount of binding activity existed whether or not ... | 1988 | 3403511 |
| [formation of a methylotrophic denitrifying biocenosis in a system of sewage treatment for nitrates]. | a methylotrophic denitrifying bioenosis composed of hyphomicrobes and paracocci was isolated from the active ooze in a system of sewage purification from nitrates. the morphological and physiological characteristics of the isolated hyphomicrobium sp. z-115 and paracoccus denitrificans z-100 and z-121 strains differed from those of the type strains, which made it difficult to identify them and to isolate them as a pure culture. this should be taken into account while determining the agents operat ... | 1988 | 3419370 |
| immunochemical probing of the structure and cofactor of nadh dehydrogenase from paracoccus denitrificans. | monospecific antibody to the respiratory nadh dehydrogenase from paracoccus denitrificans was prepared by using as antigen specific immunoprecipitates containing nadh dehydrogenase which were excised from crossed-immunoelectrophoresis plates. the latter were run with selectively solubilized plasma membranes and antibodies against plasma membranes. the antibody immunoprecipitated nadh dehydrogenase from p. denitrificans membranes biosynthetically labelled with 14c and solubilized with a wide rang ... | 1987 | 3446183 |
| modeling of microbial substrate conversion, growth and product formation in a recycling fermentor. | paracoccus denitrificans and bacillus licheniformis were grown in a carbon- and energy source-limited recycling fermentor with 100% biomass feedback. experimental data for biomass accumulation and product formation as well as rates of carbon dioxide evolution and oxygen consumption were used in a parameter optimization procedure. this procedure was applied on a model which describes biomass growth as a linear function of the substrate consumption rate and the rate of product formation as a linea ... | 1986 | 3532947 |
| redox properties of the quinoprotein methylamine dehydrogenase from paracoccus denitrificans. | paracoccus denitrificans synthesizes a methylamine dehydrogenase that contains a covalently bound form of pyrroloquinoline quinone as a prosthetic group [husain, m., & davison, v.l. (1987) j. bacteriol. 169, 1712-1717]. anaerobic reductive titration of this enzyme with dithionite proceeded through a semiquinone intermediate with spectral properties quite distinct from those of the oxidized and reduced species. from these data the molar extinction coefficients were calculated at various wavelengt ... | 1987 | 3651442 |
| a novel prenyltransferase from paracoccus denitrificans. | a new polyprenyltransferase catalysing the formation of z-double bonds was found and partially purified from extracts of paracoccus denitrificans. the enzyme catalysed a consecutive condensation of isopentenyl diphosphate with ee-farnesyl diphosphate as a primer to produce ee-farnesyl-all-z-hexaprenyl diphosphate (ze-mixed nonaprenyl diphosphate) as the final product. not only ee-farnesyl diphosphate but also neryl diphosphate, ze-farnesyl diphosphate, zee-geranylgeranyl diphosphate and zzee-pen ... | 1986 | 3707524 |
| electron transfer flavoprotein from methylophilus methylotrophus: properties, comparison with other electron transfer flavoproteins, and regulation of expression by carbon source. | when grown on methylated amines as a carbon source, methylophilus methylotrophus synthesizes an electron transfer flavoprotein (etf) which is the natural electron acceptor of trimethylamine dehydrogenase. it is composed of two dissimilar subunits of 38,000 and 42,000 daltons and 1 mol of flavin adenine dinucleotide. it was reduced by trimethylamine dehydrogenase to a stable anionic semiquinone form, which could not be converted, either enzymatically or chemically, to the fully reduced dihydroqui ... | 1986 | 3711024 |
| properties of paracoccus denitrificans amicyanin. | paracoccus denitrificans synthesizes an inducible, periplasmic, blue copper protein [husain, m., & davidson, v.l. (1985) j. biol. chem. 260, 14626-14629] that can be classified as an amicyanin on the basis of its ability to accept electrons from methylamine dehydrogenase. the amino acid composition and sequence of the 10 n-terminal residues of this protein have been determined. from these data, it is evident that amicyanin is structurally distinct from azurins as it contains no disulfide bond an ... | 1986 | 3718960 |
| the respiratory nitrate reductase from paracoccus denitrificans. molecular characterisation and kinetic properties. | the respiratory nitrate reductase from paracoccus denitrificans has been purified in the non-ionic detergent nonidet p-40. the enzyme comprises three polypeptides, alpha, beta and gamma with estimated relative molecular masses of 127 000, 61 000 and 21 000. duroquinol or reduced-viologen compounds acted as the reducing substrates. the nitrate reductase contained a b-type cytochrome that was reduced by duroquinol and oxidised by nitrate. a preparation of the enzyme that lacked both detectable b-t ... | 1986 | 3732277 |
| iron-dependent production of a heat-modifiable, 23,000-mr outer membrane protein in paracoccus denitrificans. | production of a 23,000-mr major outer membrane protein of paracoccus denitrificans atcc 13543 was dependent upon the addition of iron to a succinate-salts medium. the 23,000-mr protein was not produced in an iron-deficient medium, but production of five outer membrane proteins in the 85,000- to 72,000-mr range and of catechol were induced. the 23,000-mr protein was not produced in a complex medium even when ferric citrate was added to the medium. production of the protein was influenced by the c ... | 1986 | 3759911 |
| preliminary x-ray crystallographic study of amicyanin from paracoccus denitrificans. | single crystals have been prepared of paracoccus denitrificans amicyanin, a blue copper protein that serves as an electron acceptor for methylamine dehydrogenase. the crystals belong to the monoclinic space group p2(1), and have unit cell parameters a = 20.90 a, b = 56.61 a, c = 27.55 a and beta = 96.41. there is one molecule in the asymmetric unit. the crystals diffract to beyond 1.5 a resolution. | 1986 | 3783676 |
| is the ubiquinone pool in the respiratory chain of the bacterium paracoccus denitrificans really unhomogeneous? | we have established the participation of a mobile redox pool in the respiratory chain of anaerobically grown bacterium paracoccus denitrificans. in testing the kinetical homogeneity of the pool it was found that the ratio of fluxes of electron transport toward the terminal acceptors oxygen and nitrate was coincident for the respiratory substrates nadh and succinate; this provides evidence against the preferential link of one dehydrogenase with a distinct terminal enzyme through the separate pool ... | 1987 | 3813563 |
| succinate dehydrogenase in rhodopseudomonas sphaeroides: subunit composition and immunocross-reactivity with other related bacteria. | antibodies were raised against the succinate dehydrogenase (sdh) present in the chromatophores of phototrophically grown rhodopseudomonas sphaeroides. crossed immunoelectrophoresis experiments indicated that the sdh present in the cytoplasmic membranes of heterotrophically grown r. sphaeroides is probably the same enzyme observed in the chromatophores. the enzyme was extracted by triton x-100 in a form which consisted of only two subunits (molecular weight, 68,000 and 30,000) and was not associa ... | 1985 | 3874866 |
| structural relationships between the nadh dehydrogenases of paracoccus denitrificans and bovine heart mitochondria as revealed by immunological cross-reactivities. | an antibody raised against two subunits (mr 48 000 and 25 000) of nadh dehydrogenase from paracoccus denitrificans cross-reacts with one of more than 20 polypeptides that form the bovine heart mitochondrial nadh dehydrogenase. the cross-reacting subunit has mr 51 000 and is believed to be the nadh-binding subunit of the enzyme. antibodies raised against certain subunits of the bovine heart nadh dehydrogenase were tested for cross-reactivity with p. denitrificans cytoplasmic membranes. of those t ... | 1986 | 3956724 |
| production of superoxide anions in paracoccus denitrificans. | | 1980 | 6250489 |
| mechanism and stereospecificity of the parabactin-mediated iron-transport system in paracoccus denitrificans. | the mechanism by which paracoccus denitrificans utilizes parabactin in its iron-transport apparatus is examined. the cellular uptake of 55fe-parabactin, its enantiomer, as well as a large number of its homologues and analogues are measured. furthermore, the ability of these catecholamide ligands to stimulate microbial growth is also determined. the results of these studies point out several structural boundary conditions which the microorganism sets when utilizing iron chelates. | 1985 | 4008483 |
| the orientation of cytochromes in membrane multilayers prepared from aerobically grown escherichia coli k12. | centrifugation of membrane vesicles, prepared from ultrasonically disrupted escherichia coli k12, on to a planar surface followed by slow, partial dehydration results in a high degree of parallel orientation of the membrane planes with respect to each other and the supporting surface. rotation of such membrane multilayers about a single axis parallel with the membrane planes within the magnetic field of an electron paramagnetic resonance (e.p.r.) spectrometer allows the orientation of anisotropi ... | 1980 | 6251159 |
| [microflora in swine slurry as a parameter in determining the efficiency of deodorizing treatment. i. continuous and discontinuous aeration treatment]. | liquid manure from the pig, fresh and stored, was treated by continuous and discontinuous aeration. the purpose was to achieve a biologically stabilized and deodorized organic material. the samples which were aerated intermittently had good decomposition rates for nitrogen compounds and even so good deodorization effects. in spite of a minimum of dissolved oxygen of appr. 0.2 mg/l, good deodorization could be achieved by intermittent aeration for 23 days. simultaneous processes of nitrification ... | 1985 | 4050182 |
| the primary structures of pseudomonas am1 amicyanin and pseudoazurin. two new sequence classes of blue copper proteins. | the amino acid sequences of two blue copper proteins from the pink facultative methylotroph pseudomonas am1 (n.c.i.b. 9133) were determined. they each consist of a single polypeptide chain and bind one copper atom. amicyanin contains 99 and pseudoazurin 123 residues. copper-binding sites, consisting of the side chains of two histidine, one cysteine and one methionine residues, can be recognized in each protein by analogy with azurin and plastocyanin, but the spacings of the ligand residues are d ... | 1985 | 4091802 |
| respiration-driven proton translocation in micrococcus denitrificans. | | 1971 | 5135306 |
| release of respiratory control in particles from micrococcus denitrificans by ion-translocating antibiotics. | | 1971 | 5139216 |
| [the influence of x-irradiation on growth and respiration of micrococcus denitrificans beijerinck]. | | 1971 | 5172514 |
| heme aa3-type cytochrome c oxidases from bacteria. | | 1980 | 6263325 |
| fluorescent probe environment and the structural and charge changes in energy coupling of mitochondrial membranes. | the use of fluorescent probes to give continuous readouts of the structural states of mitochondrial membranes during energy coupling seems a logical extension of their use in the study of protein structural changes. a clear correlation of the probes' fluorescence characteristics with the acquisition of energy coupling can be demonstrated in fragmented and natural membrane using 1-anilinonaphthalene-8-sulfonate (ans) and ethidium bromide respectively. the present contribution attempts to bring to ... | 1970 | 5289010 |
| 1h nmr spectroscopy of paracoccus denitrificans cytochrome c-550. | the 1h nmr spectra of ferri- and ferro-cytochrome c-550 from paracoccus denitrificans (atcc 13543) have been investigated at 300 mhz. the ferri-cytochrome c-550 shows hyperfine-shifted heme methyl resonances at 29.90, 29.10, 16.70, and 12.95 ppm and a ligand methionyl methyl resonance at -15.80 ppm (ph 8 and 23 degrees c). four ph-linked structural transitions were detected in spectra taken as a function of ph. the transitions have been interpreted as loss of the histidine heme ligand (pk less t ... | 1984 | 6087896 |
| patterns of product inhibition for bacterial nitrite reductase. | product inhibition has been examined in the turnover kinetics of cytochrome cd1 from pseudomonas aeruginosa (atcc 19429) and from paracoccus denitrificans1 (atcc 13456). a common characteristic was a decrease in rate during the time course of assays that was not due to substrate depletion or irreversible inactivation. the product of nitrite reduction, nitric oxide (no), acted as a product inhibitor in anaerobic assays with an apparent ki of 0.2 microm, but only if the enzyme was first preincubat ... | 1984 | 6091654 |
| electron-transfer reactions of photoreduced flavin analogues with c-type cytochromes: quantitation of steric and electrostatic factors. | we have found correlations between rate constants and the difference in redox potential of the reactants for electron-transfer reactions between oxidized cytochromes and either photoproduced riboflavin or flavin mononucleotide (fmn) semiquinones (the latter rate constants extrapolated to infinite ionic strength). the riboflavin-cytochrome rate constants are about 70% of those for reduction by lumiflavin, probably because of steric interference by the ribityl side chain. reduction of cytochromes ... | 1984 | 6093864 |
| isolation and characterization of megaplasmid dna from lithoautotrophic bacteria. | a method is described for the preparative isolation of megaplasmids ranging in size from 340 to 700 kb. these plasmids were isolated from chemolithoautotrophic bacteria including the species alcaligenes, pseudomonas, and paracoccus. the procedure was based on alkaline sodium dodecyl sulfate lysis of the cells, followed by heat treatment, salt precipitation, several phenol extractions, dialysis steps, and proteinase and rnase treatment. the various parameters were evaluated and controlled. hydrog ... | 1984 | 6098903 |
| immunochemical relationship of the major outer membrane protein of rhodopseudomonas sphaeroides 2.4.1 to proteins of other photosynthetic bacteria. | immunoblots of sodium dodecyl sulfate-polyacrylamide gels derived from outer membrane preparations of various strains of rhodopseudomonas sphaeroides revealed polypeptides which cross-reacted with antibody directed against the major outer membrane protein of r. sphaeroides 2.4.1. immunochemical quantitation of the major outer membrane protein of strain 2.4.1 showed approximately 5.5 x 10(4) molecules per cell whether cells were grown chemoheterotrophically or photoheterotrophically. rhodospirill ... | 1983 | 6188744 |
| effect of various sources of organic carbon and high nitrite and nitrate concentrations on the selection of denitrifying bacteria. ii. continuous cultures in packed bed reactors. | the effect of different organic compounds, nitrites and nitrates at the concentration of 1,000 mg n/l on the quantitative and strain-specific selection of denitrifying bacteria was determined in anaerobic packed bed reactors. both the source of carbon and nitrogen form influenced strain specificity and the frequency of occurrence of denitrifying bacteria. the frequency of denitrifying bacteria within packed bed reactor ranged in different media from 11% (glucose and nitrates) to 100% (methanol a ... | 1983 | 6194668 |
| changes in the cytochrome c content during the aerobic adaptation of paracoccus denitrificans. | concentration of cytochrome c decreases when shaking anaerobically grown cells in a non-growth medium down to 50% of the original amount in the cell, depending on the degree of aeration. only 10-20% of this amount can be found in the adaptation medium. the main portion of cytochrome c is degraded during the adaptation. inhibitors of proteinases do not influence the degradation. addition of mammalian cytochrome c fully prevents the degradation of bacterial cytochrome c but not its release from ce ... | 1982 | 6277754 |
| the antibacterial action of tinopal an. | the bactericidal activity of tinopal an [1,1-bis(3,n-5-dimethyl-benzoxazol-2-yl)-methine p-toluene sulphonate] was shown to be due to a mechanism entirely independent of its inhibitory effects upon nadh dehydrogenase which were reported previously. whereas the compound had no significant effect upon dna synthesis in escherichia coli d22, rna and protein synthesis were immediately and markedly inhibited. in confirmation, tinopal an caused an immediate cessation in inducible beta-galactosidase syn ... | 1984 | 6206200 |
| a two-subunit cytochrome c oxidase (cytochrome aa3) from paracoccus dentrificans. | cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, ec 1.9.3.1) was purified from the cytoplasmic membrane of the bacterium paracoccus denitrificans. the enzyme contains two heme groups (a and a3) and two copper atoms per minimal unit, oxidizes mammalian cytochrome c at a high rate, and, when incorporated into liposomes, generates an electrochemical proton gradient during cytochrome c oxidation. sodium dodecyl sulfate/polyacrylamide gel electrophoresis reveals only two subunits of ap ... | 1980 | 6244543 |
| cytochrome c and the evolution of energy metabolism. | | 1980 | 6244618 |
| effects of monoclonal antibodies to bovine and paracoccus denitrificans cytochromes c on reactions with oxidase, reductase and peroxidase. | the effects of monoclonal antibodies to bovine and paracoccus denitrificans cytochromes c (kuo, l.m. and davies, h.c. (1983) mol. immunol. 20, 827-838) in the reactions of the cytochromes c with cytochrome c oxidase, reductase and peroxidase were studied. spectrophotometric assays were employed, under conditions where binding of cytochrome c to the enzymes appears to be rate-limiting. less than stoichiometric amounts of antibodies to p. denitrificans cytochrome c added to the cytochrome rendered ... | 1984 | 6205693 |
| on the structure of heme d1. an isobacteriochlorin derivative as the prosthetic group of dissimilatory nitrite reductase? | timkovich and co-workers have recently proposed a chlorin macrocycle structure for the heme d1 prosthetic group isolated from cytochrome cd1 of pseudomonas aeruginosa and paracoccus denitrificans (timkovich, r., cork, m. s., and taylor, p. v. (1984) j. biol. chem. 259, 1577-1585; 15089-15093). however, this chlorin structure deduced by them is not entirely consistent with the spectral data. an alternative structure is proposed here based on the available spectral evidence. it is suggested that h ... | 1985 | 3926768 |
| on the dielectrically observable consequences of the diffusional motions of lipids and proteins in membranes. 2. experiments with microbial cells, protoplasts and membrane vesicles. | the dielectric properties of suspensions of intact cells of methylophilus methylotrophus, paracoccus denitrificans and bacillus subtilis have been measured in the frequency range 1 khz to 13 mhz. all possess a pronounced dispersion corresponding in magnitude and relaxation time to the "beta-dispersion" in a terminology defined by schwan [adv. biol. med. phys. 5:147-209 (1957)]. the latter two strains, but not m. methylotrophus, also possess a substantial alpha-dispersion. the relaxation time of ... | 1985 | 3935420 |
| purification and properties of thiamine pyrophosphokinase in paracoccus denitrificans. | the existence of thiamine pyrophosphokinase [ec 2.7.6.2] in procaryotic cells was first demonstrated in paracoccus denitrificans (j. bacteriol, (1976) 126, 1030-1036). the enzyme was therefore purified from this organism to determine its molecular structure and properties. thiamine pyrophosphokinase which was purified 620-fold from p. denitrificans showed a single band on both polyacrylamide and sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis, and the molecular weight in the latt ... | 1980 | 6251035 |
| the use of bee venom melittin to assess the topography of membrane vesicles derived from paracoccus denitrificans. | there exists considerable controversy regarding membrane topography in vesicles derived by osmotic lysis of spheroplasts of gram-negative bacteria. it has been reported by others that bee venom can be used to quantitate the portion of a heterogeneous vesicle population with an inside-out orientation by determining the degree of loss of crypticity of nadh dehydrogenase activity. we have demonstrated that a major component of bee venom, melittin, causes an increase in the activity of several diffe ... | 1980 | 6257350 |
| phase separation of integral membrane proteins in triton x-114 solution. | a solution of the nonionic detergent triton x-114 is homogeneous at 0 degrees c but separates in an aqueous phase and a detergent phase above 20 degrees c. the extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. the partition of proteins during phase separation in solutions of triton x-114 is investigated. hydrophilic proteins are found exclusively in the aqueous phase, and integral membrane proteins with an amphiphilic ... | 1981 | 6257680 |
| bacterial microflora participating in the removal of nitrogen from industrial wastewaters by nitrification and denitrifaction. | nitrification and denitrification were used to purify industrial nitrogenous-organic wastewaters containing 1,130 mg n/l (mainly in the form of ammonium and urea) and with mean cod 1,050 mg o2/l. the use of a five-step system consisting of two denitrifying beds and three nitrifying activated sludge reactors gave 99% removal of nitrogen and 54% reduction in cod during total retention time of the wastes of 81 hours. the participation of stage i nitrifying bacteria in the activated sludge was 4.4 t ... | 1984 | 6205553 |
| eubacteria have 3 growth modes keyed to nutrient flow. consequences for the concept of maintenance and maximal growth yield. | aerobic growth of escherichia coli and paracoccus denitrificans has been studied in chemostat, fed batch, and recycling fermentor modes under carbon and energy limitation. two abrupt drops or discontinuities in molar growth yield, y, have been found that occur over relatively short ranges in the value of specific growth rate. before the first discontinuity, y is constant and maximal. after the first discontinuity, at a doubling time of 33 h, y becomes constant again and independent of mu until t ... | 1984 | 6202266 |
| a periplasmic location for methanol dehydrogenase from paracoccus denitrificans: implications for proton pumping by cytochrome aa3. | | 1981 | 6261750 |
| a comparison of the respiratory chain in particles from paracoccus denitrificans and bovine heart mitochondria by epr spectroscopy. | a study is presented on the epr characteristics of the paramagnetic groups in the respiratory chain present in membrane particles of paracoccus denitrificans, the respiratory system of which is very similar to that in submitochondrial particles from beef heart. all paramagnetic prosthetic groups of the mitochondrial system are also found in the bacterial plasma membrane. their properties suggest that the respiratory groups are embedded in very similar protein environments in the two systems. | 1980 | 6263319 |
| a specific uncoupler-binding protein in tetrahymena pyriformis and paracoccus denitrificans. | the uncoupler of mitochondrial oxidative phosphorylation, 2-nitro-4-azido-carbonylcyanide phenylhydrazone (n3ccp) which is capable of photoaffinity labeling has been used to examine the effect of uncouplers on the energy conserving membrane of paracoccus denitrificans and tetrahymena pyriformis. the n3ccp uncouples respiration in p. denitrificans and t. pyriformis cells with u1/2 values of 1.05 microm and 0.24 microm, respectively. binding studies show the presence of 0.65 +/- 0.05 high affinity ... | 1980 | 6263320 |
| proton pump coupled to cytochrome c oxidase in paracoccus denitrificans. | the proton translocating properties of cytochrome c oxidase in whole cells of paracoccus denitrificans have been studied with the oxidant pulse method. leads to h+/2e- quotients have been measured with endogenous substrates, added methanol and added ascorbate (+tmpd) as reductants, and oxygen and ferricyanide as oxidants. it was found that both the observed leads to h+/o with ascorbate (+tmpd) as reductant, and the differences in proton ejection between oxygen-and ferricyanide pulses, with endog ... | 1981 | 6263335 |
| isolation and characterization of paracoccus denitrificans mutants with defects in the metabolism of one-carbon compounds. | mutants deficient in the metabolism of one-carbon compounds have been obtained by treating paracoccus denitrificans with the mutagen n-methyl-n'-nitro-n-nitrosoguanidine. mutants were selected without enrichment procedures by newly developed plate screening tests. the obtained mutants were characterized by their growth responses, cytochrome composition, enzyme activities, and immunogenic reaction with antisera against methanol dehydrogenase. by these criteria five mutant classes could be disting ... | 1985 | 3905763 |
| electron transport to nitrous oxide in paracoccus denitrificans. | | 1980 | 6248362 |
| study of 'difficult peptides' from paracoccus cytochrome c-550 and a dolphin cytochrome c. fast atom bombardment: a new method for molecular weight and sequence determination of peptides. | | 1981 | 6268447 |
| reaction of oxygen with cytochrome c oxidase from paracoccus denitrificans. | the reaction of reduced cytochrome c oxidase (ec 1.9.3.1) from paracoccus denitrificans (american type culture collection 13543) with dioxygen has been followed by laser flash photolysis of the co derivative. in detergent-stabilized solutions the reaction showed at least two distinct kinetic components, the faster of which was oxygen concentration dependent and had a rate of approximately 60 x 10(6) m-1 s-1. the slower reaction was independent of oxygen concentration and had a rate of 9 x 10(2) ... | 1981 | 6268625 |
| low temperature kinetic studies on rat liver mitochondria containing covalently linked derivatives of cytochrome c. | | 1980 | 6248514 |
| a reassessment of the structure of paracoccus cytochrome c-550. | | 1981 | 6270339 |
| structural features and the reaction mechanism of cytochrome oxidase: iron and copper x-ray absorption fine structure. | x-ray edge absorption of copper and extended fine structure studies of both copper and iron centers have been made of cytochrome oxidase from beef heart, paracoccus dentrificans, and hb-8 thermophilic bacteria (1-2.5 mm in heme). the desired redox state (fully oxidized, reduced co, mixed valence formate and co) in the x-ray beam was controlled by low temperature (-140 degrees c) and was continuously monitored by simultaneous optical spectroscopy and by electron paramagnetic resonance (epr) monit ... | 1981 | 6264990 |
| resonance raman spectra of cytochromes c and b in paracoccus denitrificans membranes: evidence for heme--heme interactions. | resonance raman (rr) scattering from cytochromes b and c in the bacterium paracoccus denitrificans was recorded by exciting with the 568.2-, 530.9-, and 520.8-nm lines of a kr+ laser. the main features of the spectra were similar to those of the analogous cytochrome b-c1 complex derived from pigeon breast mitochondria. differences in the 1300-cm-1 region were interpreted in terms of marker bands for heme type and spectral coupling of the hemes on the membranes. it is difficult to explain the res ... | 1981 | 6275884 |
| copper and manganese electron spin resonance studies of cytochrome c oxidase from paracoccus denitrificans. | the two-subunit cytochrome c oxidase from paracoccus denitrificans contains two heme a groups and two copper atoms. however, when the enzyme is isolated from cells grown on a commonly employed medium, its electron paramagnetic resonance (epr) spectrum reveals not only a cu(ii) powder pattern, but also a hyperfine pattern from tightly bound mn(ii). the pure mn(ii) spectrum is observed at -40 degrees c; the pure cu(ii) spectrum can be seen with cytochrome c oxidase from p. denitrificans cells that ... | 1981 | 6269600 |
| solubilized cytochrome c oxidase from paracoccus denitrificans is a monomer. | cytochrome c oxidase purified from the bacterium paracoccus denitrificans was analyzed by analytical ultracentrifugation. in the detergent octyltetra/pentaoxyethylene (c8e45), the isolated enzyme exhibits a molecular weight of 79,000 to 84,000. the detergent-solubilized enzyme is thus a monomer which contains one copy of each of the two subunits. | 1982 | 6279605 |