| effects of an arkansas strain of infectious bronchitis vaccine on the head-associated lymphoid tissue (halt). | chicks were vaccinated with an arkansas strain of infectious bronchitis virus (ibv) vaccine when they were 1 day (trial 1) or 4 weeks old (trial 2). starting at 4 weeks 3 days of age, chicks in both trials were subjected to an assay that measures the immunofunctional response of the gland of harder (gh), one of the components of the head-associated lymphoid tissue (halt). the assay involved multiple ocular exposures to killed brucella abortus antigen, after which tears were collected and titered ... | 1991 | 1649590 |
| antigen-specific igg2a production in response to prostaglandin e2, immune complexes, and ifn-gamma. | immune complexes (ic) can inhibit the differentiation of b lymphocytes into igm secreting plasma cells in both ag-specific and polyclonal systems. this report describes the ability of ic and ifn-gamma, or ifn-gamma and pge2 to regulate the class of ig produced in an ag-specific system. in an in vitro model system using fluorescein (fl)-brucella abortus as ag, we previously showed that ic composed of an anti-fl antibody and fl-ag inhibited the ability of fl-specific b cells to develop into igm pl ... | 1991 | 1655892 |
| mutagenesis of brucella abortus: comparative efficiency of three transposon delivery systems. | three transposon-containing 'suicide' plasmids were evaluated for their efficiency in promoting insertional mutagenesis in brucella abortus. all three plasmids are mobilizable vectors and so were conjugally transferred from mobilizing strains of the donor escherichia coli. the transposition frequencies were expressed as the ratio of kmr exconjugants per total recipient cells at the initiation of mating. the put/km-based delivery system, which utilizes a derivative of tn5, yielded a very high fre ... | 1991 | 1665539 |
| a polyclonal model for b cell tolerance. i. fc-dependent and fc-independent induction of nonresponsiveness by pretreatment of normal splenic b cells with anti-ig. | we have developed a simple and adaptable, polyclonal model for b cell nonresponsiveness that is based on the inhibitory activity of anti-ig as a surrogate for ag. in our system the induction phase (treatment with anti-ig) is separated from the challenge phase (ag or mitogen), so that the critical events in each phase can be evaluated. our results show that t cell-depleted b cells precultured for 18 to 24 h with rabbit anti-ig reagents are rendered unresponsive to challenge with either ag, fluore ... | 1991 | 1672346 |
| production of a novel antigen by conjugation of hiv-1 to brucella abortus: studies of immunogenicity, isotype analysis, t-cell dependency, and syncytia inhibition. | in the present study inactivated human immunodeficiency virus type 1 (hiv-1) was conjugated to brucella abortus and tested for immunogenicity in normal and anti-l3t4-treated balb/c mice. hiv-ba was more immunogenic than uncoupled hiv in normal mice, since 6-fold less virus in hiv-ba preparations elicited higher titer responses than hiv-1 alone. furthermore, the hiv-ba antibody response reached higher levels before the hiv-1 response. immunoblot analysis showed that most of the hiv-1 antigens wer ... | 1991 | 1678617 |
| the bovine p150/95 molecule (cd11c/cd18) functions in primary cell-cell interaction. | the monoclonal antibody (mab), c5b6, recognizes the cd11c/cd18 molecule on the surface of bovine peripheral blood monocytes. c5b6 was reactive with 69-83% monocytes, all granulocytes, and less than 5% of lymphocytes from cattle. of the lymphocyte series, the antibody had specificity for large lymphocytes and two lines expressing t cell markers, but was not reactive with small lymphocytes, thymocytes, a tumor cell line of b-cell lineage, an interleukin 2 (il2)-dependent t cell line, fibroblasts, ... | 1991 | 1683051 |
| antigenic relationships of the lipopolysaccharides of escherichia hermannii strains with those of escherichia coli o157:h7, brucella melitensis, and brucella abortus. | clinical isolates of escherichia hermannii which showed serological cross-reaction with polyclonal antisera to the o-polysaccharide portion of the lipopolysaccharide of e. coli o157 strains and with antisera to the o antigens of brucella abortus and b. melitensis were found by chemical and nuclear magnetic resonance analyses to have lipopolysaccharide o chains composed of linear polymers containing 1,2- and 1,3-linked 4-acetamido-4,6-dideoxy-alpha-d-mannopyranosyl (alpha-d-rhap4nac) residues. tw ... | 1990 | 1691146 |
| identification of seven surface-exposed brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay. | a panel of monoclonal antibodies (mabs) to seven brucella outer membrane proteins were characterized. these antibodies were obtained by immunizing mice with sodium dodecyl sulfate-insoluble (sds-i) fractions, cell walls, or whole bacterial cells of brucella abortus or b. melitensis. enzyme-linked immunosorbent assays were used to screen the hybridoma supernatants and to determine their binding at the surface of rough and smooth b. abortus and b. melitensis cells. the outer membrane proteins (omp ... | 1990 | 1701417 |
| [cloning of brucella genes in escherichia coli k12 cells and analysis of products of the cloned genes]. | the libraries of brucella melitensis 565 and brucella abortus 99 in escherichia coli cells have been constructed. some clones of escherichia coli producing the specific brucella antigens have been found in immunological tests with brucella antiserum. two strains producing antigens have been characterized, one being from brucella melitensis 565 and another from brucella abortus 99 clone libraries . both strains synthesize two antigens that were studied by immunoelectrophoresis, immunoblotting aft ... | 1990 | 1701518 |
| selective immunosuppression of prodigiosin 25-c and fk506 in the murine immune system. | the immunosuppressive effects of prodigiosin 25-c were studied in comparison with fk506. both prodigiosin 25-c and fk506 suppressed t cell proliferation in response to concanavalin a (con a) or phytohemagglutinin (pha) more significantly than that to lipopolysaccharide. however, prodigiosin 25-c inhibited con a-mediated mitogenic response more strongly than pha-mediated one. fk506 showed no selectivity among those responses. in addition, when higher concentration of con a was used an inhibitory ... | 1990 | 1701765 |
| [the antigenic relationship between brucella abortus, brucella melitensis and yersinia enterocolitica serotype 0:3 and 0:9]. | antigenic interrelationship between b. abortus, b. melitensis, y. enterocolitica serotype 0:3 and serotype 0:9 were investigated in hyperimmün sera obtained from rabbits, by using agglutination and agglutinin absorbtion tests. agglutination was determined at 1/2560 titer of b. abortus and b. melitensis hyperimmün sera when their own antigens were used. on the other hand, these sera agglutinated y. enterocolitica serotype 0:9 antigen at 1/1280 and 1/320 titer respectively. as it was seen that hig ... | 1990 | 1704479 |
| molecular modeling of antibody-antigen complexes between the brucella abortus o-chain polysaccharide and a specific monoclonal antibody. | a molecular model of the binding site of an anti-carbohydrate antibody (yst9.1) has been developed using computer-assisted modeling techniques and molecular dynamics calculations. sequence homologies among yst9.1 and the fv regions of mcpc603, j539 and human bence--jones protein rei, all of which have solved crystal structures, provided the basis for the modeling. the groove-type combining site model had a topography which was complementary to low energy conformers of the polysaccharide, a bruce ... | 1991 | 1715561 |
| molecular characterization and proposal of a neotype strain for bartonella bacilliformis. | bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by dna hybridization, guanine-plus-cytosine content, genome size, and 16s rrna sequencing. dnas from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55 degrees c (optimal reassociation criterion) and 100% relatedness in reactions done at 70 degrees c (stringent reassociation criterion). there was no evidence of divergence within the related sequences ... | 1991 | 1715879 |
| 16s rrna sequences of bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class proteobacteria. | the primary structures of 16s rrnas of bartonella bacilliformis, an isolate of the cat scratch disease (csd) bacillus, and a strain phenotypically similar to the csd bacillus were determined by reverse transcriptase sequencing. these microorganisms were found to be members of the alpha-2 subgroup of the class proteobacteria. the sequence from b. bacilliformis was most closely related to the rrna of rochalimaea quintana (91.7% homology), the etiologic agent of trench fever. the sequence from the ... | 1991 | 1719021 |
| human brucellosis: immunoblotting analysis of three brucella abortus antigenic fractions allows the detection of components of diagnostic importance. | results indicating that analysis of the immune humoral response of brucellosis patients by immunoblotting provides useful information for the characterization of antigenic fractions of possible diagnostic importance in human brucellosis are presented. sera of 90 patients were obtained: 23 suffering from chronic brucellosis, 20 from acute brucellosis and 47 belonging to the group of serologically positive individuals (spi) without clinical evidence of active infection at the time of examination, ... | 1991 | 1726568 |
| effects of cigarette smoke on the immune response. ii. chronic exposure to cigarette smoke inhibits surface immunoglobulin-mediated responses in b cells. | we have previously reported that chronic exposure of rats to cigarette smoke inhibits the antibody-forming cell (afc) response to both t-dependent and t-independent antigens and may reflect b cell dysfunction. in this communication we extend these studies to show that t cell functions are normal in chronically smoke-exposed rats (sm) as judged by their responses to mitogens and "nominal" or alloantigens. while b cells from sm respond significantly to the b cell mitogen lipopolysaccharide (lps), ... | 1991 | 1746027 |
| research note: effects of age and immunization interval on the anamnestic response to t-cell-dependent and t-cell-independent antigens in chickens. | measurement of a humoral anamnestic response is a common procedure used to evaluate immunological competence. the purposes of the present study were to determine the optimum time period between primary and secondary immunizations needed to obtain the maximum antibody titer and to determine whether this interval differs with the age of the chicken or with the type of inducing antigen. the anamnestic responses of birds of two ages to a t-cell-dependent antigen (srbc) and to a t-cell-independent an ... | 1991 | 1754551 |
| in vivo effects of trh, t3 and cgh on antibody production and t- and b-lymphocytes proliferation in immature male chickens. | newly hatched white leghorn male chicks were used in this study. different doses of t3 (0.1 or 1 ppm) or trh (1 or 5 ppm) were administered in the feed for an 8-week period. chicken growth hormone (cgh) (10 micrograms/kg bw) was injected (i.v.) into a different group of chicks twice daily for 1 week starting at 7 weeks of age. a different group received both t3 (0.1 and 1 ppm) and cgh. serum concentrations of t4, t3 and gh, antibody production against sheep red blood cells (srbc) and brucella ab ... | 1991 | 1757115 |
| [the possibility of creating a vaccinal strain of brucella abortus 19-ba with multiple antibiotic resistance]. | the possibility of preparing b. abortus vaccine strain 19-ba with multiresistance to antibiotics was shown. the strain was obtained by the spontaneous induction of resistance to rifampicin with the subsequent transformation of nonconjugative hybrid plasmid povi which, in addition to rifampicin resistance, governed the resistance of brucellae to tetracycline, doxycycline, ampicillin, and streptomycin. experiments on guinea pigs demonstrated the immunization with both multiresistant vaccine strain ... | 1991 | 1759514 |
| effect of endocytic and metabolic inhibitors on the internalization and intracellular growth of brucella abortus in vero cells. | uptake, transfer to rough endoplasmic reticulum, and intracellular growth of brucella abortus were studied in vero cells treated with endocytic and metabolic inhibitors. infection of vero cells was suppressed when inhibitors of energy metabolism (iodoacetate, dinitrophenol), receptor-mediated endocytosis (monodansylcadaverine, amantadine, methylamine), or endosomal acidification (chloroquine, ammonium chloride, monensin) were added to the inoculum. inhibition was not observed when these drugs we ... | 1991 | 1767988 |
| effects of stage of gestation and breed on bovine responses to vaccination with brucella abortus strain 19. | eighty-eight cattle were injected sc with 2.5 x 10(8) viable cells of brucella abortus strain 19. all but 1 heifer became seropositive on the basis of the results of 7 brucellosis tests, and the proportion positive decreased with time. the proportion of cattle that were seropositive during a 20- to 67-week period after vaccination was as follows, in decreasing order: hemolysis-in-gel, 59%; buffered-acid plate antigen, 39%; elisa, 16%; card, 10%; rivanol, 8%; cold complement-fixation, 7%; and aut ... | 1991 | 1769876 |
| brucella abortus-associated meningitis in aborted bovine fetuses. | granulomatous meningitis was present in 6/33 bovine fetuses from which brucella abortus (b. abortus) had been isolated. meningitis was severe in three fetuses, moderate in one fetus, and mild in the remaining two fetuses. the meningitis was characterized by the infiltration of a mixed population of lymphocytes, plasma cells, and macrophages in the leptomeninges. vasculitis characterized by the infiltration of lymphocytes and plasma cells in the vascular wall was observed in the vessels of the ce ... | 1991 | 1771739 |
| immunosuppressive effects of fusarium moniliforme corn cultures in chickens. | in the present study, the toxic and immunosuppressive effects of three isolates of fusarium moniliforme (mrc 826, mrc 1069, and rrc 408) in male white leghorn chickens were investigated. chickens were fed diets containing .5, 5.0, or 25% of f. moniliforme corn cultures for 6 wk. about 30% of the chickens fed rrc 408 had leg weakness. relative weights of bursae of fabricius were lower in birds fed all doses of mrc 826. birds fed 5.0 or 25% mrc 1069 had lower relative spleen weights. thymus weight ... | 1991 | 1780260 |
| increased vascular permeability of brucella abortus bacilli in the thymus of nzb/w f1 mice. | various amounts of the bacterium, brucella abortus (ba) were injected intravenously into autoimmune nzb/w f1 mice and non-autoimmune bdf1 mice and then the localization of ba in the thymus was traced using an immunohistochemical method at 30 min and 3 h after injection. the results showed that a greater amount of ba became consistently localized in the thymic parenchyma in a free form or in a phagocytized form in nzb/w f1 mice in comparison with bdf1 mice, indicating a marked increase of vascula ... | 1991 | 1782330 |
| comparative immunopharmacology and toxicology of the bisbenzylisoquinoline alkaloids tetrandrine and berbamine. | comparison of the bisbenzylisoquinolines tetrandrine and berbamine shows that both drugs are equipotent in terms of enhancement of antibody responses and suppression of delayed-type hypersensitivity (dth) responses to sheep red blood cell antigens. both compounds are also equally active when given to mice during the induction and expression phases of dth. using a model of experimental brucellosis in mice, it was found that both compounds did not affect antibody responses, while they caused equip ... | 1991 | 1783472 |
| effect of stage of gestation on efficacy of brucella abortus strain-19 vaccination in cattle. | seventy-nine cattle in all stages of gestation were inoculated with a low dose (2.5 x 10(8) colony-forming units) of brucella abortus strain 19, then challenge exposed with pathogenic b abortus strain 2308 during the subsequent gestation. a brucellosis case was defined by isolation of strain 2308 from dam or calf samples. cumulative incidence of brucellosis cases was 48, 33, 25, or 47% for cattle that were, respectively, not pregnant, or 19 to 87, 100 to 167, or 190 to 253 days in gestation at v ... | 1991 | 1785727 |
| experimental infection of goat fetuses in utero with a stable, rough mutant of brucella abortus. | fetuses of goats in their last trimester of pregnancy were experimentally infected with brucella abortus strain rb51, a stable rough mutant deficient in the perosamine o-chain content of its lipopolysaccharide. rb51 maintained its rough phenotype in vivo and did not induce abortion. infection with rb51 resulted in the production of significant levels of igg type antibodies specific for b abortus cellular antigens distinct from the perosamine o-chain. these findings suggest that strain rb51 will ... | 1991 | 1788475 |
| effect of weaning on antibody responses and nematode parasitism in merino lambs. | lambs weaned at eight weeks old were compared with control lambs which remained with their dams; both groups grazed the same pasture. weaning significantly reduced the growth rate, control lambs being, on average, 6 kg heavier than weaned lambs at 15 weeks old. when contamination of pasture with larval parasites was light, both groups of lambs suffered only modest parasitic infections. when lambs were experimentally infected with 5000 haemonchus contortus and 10,000 trichostrongylus colubriformi ... | 1991 | 1788476 |
| augmentation of natural killer cell activity in mice by bru-pel. | the immunomodulatory and anti-tumor activity of bru-pel, an aqueous-ether extracted residue of brucella abortus (strain 456), was investigated. bru-pel was administered to c57bl/6 mice intraperitoneally (i.p.) and tested for its effect on natural killer (nk) cell activity in spleen cells, liver, and peritoneal cavity. three days after injecting 100 micrograms of bru-pel i.p., the cytotoxicity of spleen cells against yac-1 target cells, assessed by lu20 increased by approximately two-fold and non ... | 1991 | 1790138 |
| immunogenicity of lipopolysaccharide derived from brucella abortus: potential as a carrier in development of vaccines for aids. | in view of its unique ability to stimulate human b cells, we have considered using brucella abortus (ba) as a carrier for human vaccines. recently we showed that hiv-1 coupled to ba, but not unconjugated hiv-1, was able to stimulate murine responses even in the relative absence of cd4+ t cells. this result suggested that hiv-ba may be useful in boosting the immunity of individuals infected with hiv-1 and who have impaired cd4+ t cell function. in order to refine this carrier we purified lipopoly ... | 1991 | 1805568 |
| lack of fc epsilon rii expression by murine b cells after in vivo immunization is directly associated with ig secretion and not ig isotype switching. | the "low affinity" fc receptor for ige (fc epsilon rii) has been reported to be absent from normal murine and human b cells that express a membrane (m)ig isotype other than migm or migd in vivo. this would suggest that fc epsilon rii expression is specifically lost after in vivo ig isotype switching. we demonstrate that during a murine immune response to the bacterium brucella abortus, to goat anti-mouse igd (g alpha m delta) antibody, or to infection with the nematode parasites nippostrongylus ... | 1991 | 1826019 |
| immunoglobulin g binding activity of brucella abortus. | a brucella abortus protein with a molecular weight of 50 kda has been shown to bind bovine immunoglobulin g from healthy, brucellosis-free animals. the brucella immunoglobulin g binding molecule appears to be a protein, since it is susceptible to proteolysis. the protein is presumed to be located on the cell surface, since intact cells precipitate bovine immunoglobulin g. examination of other species of brucella shows that all brucella species and strains tested express the protein. b. abortus c ... | 1991 | 1826340 |
| immunopotentiation of cattle vaccinated with a soluble brucella abortus antigen with low lps content: an analysis of cellular and humoral immune responses. | the adjuvant effects of dimethyl dioctadecyl ammonium bromide (dda) alone or in combination with trehalose dimycolate (tdm) or muramyl dipeptide (mdp) on bovine humoral and cellular responses to a soluble protein extract of gamma irradiated brucella abortus strain 19 (speba) were investigated. thirty-five beef steers were randomly allotted to nine groups. three of these groups received speba (2 mg protein per dose) subcutaneously in combination with adjuvants, one group received the reduced dose ... | 1991 | 1835212 |
| the effects of adjuvants on immune responses in cattle injected with a brucella abortus soluble antigen. | five different adjuvants were examined for potentiation of humoral and cell-mediated immune (cmi) responses in cattle to a brucella abortus soluble antigen (basa). two separate experiments were performed involving a total of 64 steers, divided among six groups (experiment 1) and 9 groups (experiment 2). the adjuvants used were: muramyl dipeptide, freund's incomplete adjuvant, dimethyl-dioctadecyl ammonium bromide (dda), bordetella pertussis and propionibacterium acnes. in each experiment, three ... | 1991 | 1835213 |
| influence of antigen concentration, inoculation interval, number of exposures, type of housing, and placement concentration on the tear antibody response to brucella abortus in chickens. | a series of trials was run in leghorn chicks to examine select conditions affecting the tear antibody response to killed brucella abortus antigen given by eyedrop administration. specific conditions examined were concentration of antigen, number of antigen exposures, and interval between antigen exposures. trials were also run to determine the earliest age at which the assay was functional in both broilers and leghorns. two types of housing (isolators and battery cages) were examined, as were tw ... | 1991 | 1835376 |
| [phenol-insoluble vaccine against human brucellosis: evaluation of the immunogenic power in guinea pigs]. | a phenol insoluble extract vaccine proposed to immunize men against brucellosis was tested for its ability in protecting guinea pigs against challenge with virulent brucella abortus strain 2308. living attenuated brucella abortus strain 19 and b. melitensis rev. 1 were included in the experiment for comparison. ninety three animals were vaccinated in each group and subdivided in subgroups of 31 for challenge with 10(4), 10(3) and 10(2) colony forming units of virulent b. abortus 2308. a global a ... | 1991 | 1843392 |
| establishment of dose-response relationships in balb/c mice, using brucella cell surface protein and lipopolysaccharide. | a study was conducted to determine the immune (increased antibody) and protective (reduced colony-forming units) responses induced in mice by a: (i) single vaccinal inoculation, using various concentrations of brucella cell surface protein (bcsp) or lipopolysaccharide (lps); (ii) primary inoculation, using various concentrations of bcsp, followed by a secondary inoculation, using a standard concentration of bcsp; and (iii) primary inoculation, using 1 concentration of bcsp or lps, followed by a ... | 1991 | 1901465 |
| unresponsiveness of vaccinated balb/c mice to a second inoculation of lipopolysaccharide from brucella abortus strain 2308. | a study was conducted to determine whether the protection induced in mice by a primary inoculation of lipopolysaccharide from brucella abortus would be enhanced by a second inoculation given at different time intervals. protection was challenged by exposure of the mice to a virulent culture of b. abortus strain 2308. reduced mean viable count and/or splenic weights were the criteria of protection. there was no significant difference (p greater than 0.05) in the protective responses among mice gi ... | 1991 | 1902610 |
| superoxide dismutases of virulent and avirulent strains of brucella abortus. | extracts of brucella abortus strains 2308,rb51,45/20 and st 19 had no significant differences in superoxide dismutase (sod) activity as measured by the epinephrine assay. these b. abortus strains represent smooth, intermediate and rough colony forms. sod activity was inhibited 60 to 75% by 2 mm kcn and suggests the presence of cu/zn sod. the sod activities were similar when the strains were grown in trypticase soy broth containing either 0.5% glucose or erythritol. there were two distinct sod ac ... | 1991 | 1903225 |
| differentiation by western blotting of immune responses of cattle vaccinated with brucella abortus strain 19 or infected experimentally or naturally with virulent brucella abortus. | brucella abortus strain 19 salt-extractable proteins fractionated by differential ammonium sulfate precipitation were used in a western blotting method to detect bovine immunoglobulin g antibodies to b. abortus. sera from infected cattle and from cattle vaccinated with strain 19 and subsequently exposed to virulent b. abortus bound to a common group of antigens ranging in molecular weights from 31,000 to 45,000 daltons. immunoglobulin g antibodies in sera from the latter group in addition also b ... | 1991 | 1904667 |
| brucella abortus in bison. ii. evaluation of strain 19 vaccination of pregnant cows. | protection against brucella abortus induced abortion and infection provided by strain 19 (s19) vaccination was evaluated in american bison (bison bison). forty-eight pregnant bison were manually inoculated (mi) with s19 vaccine, 44 were ballistically inoculated (bi) with an absorbable hollow pellet containing lyophilized s19, and 46 were manually injected with buffered saline as non-vaccinated controls (nvc). all bison were brucella spp. seronegative prior to the experiment, in the second trimes ... | 1991 | 1906114 |
| effect of the novel triazinium zwitterion 1-methyl-3-phenyl-1,2,4-triazinium-5-olate on immunological responses and experimental brucellosis in mice. | the immunopharmacology of a novel triazinium zwitterion, designated jr-6, was studied in mice. experiments show dose-response inhibition of antibody and delayed-type hypersensitivity (dth) responses to sheep red blood cell antigens. suppression of dth was confirmed using trinitrochlorobenzene as a contact-sensitizing antigen. using a model of experimental brucellosis in mice, it was found that jr-6 caused suppression of specific antibody and dth reaction, as well as spleen weight, but a statisti ... | 1991 | 1906435 |
| biological properties of rb51; a stable rough strain of brucella abortus. | a rifampin-resistant mutant of brucella abortus, designated rb51, was derived by repeated passage of strain 2308 on trypticase soy supplemented with 1.5% agar and varying concentrations rifampin or penicillin. the rb51 colonies absorbed crystal violet and rb51 cell suspensions autoagglutinated, indicating a rough type colonial morphology for this strain. no o-chain component was detected in lipopolysaccharide (lps) extracted from rb51 on sds-page gels stained with silver. western blot analysis w ... | 1991 | 1908158 |
| swine immunity to an attenuated salmonella typhimurium mutant containing a recombinant plasmid which codes for production of a 31-kilodalton protein of brucella abortus. | salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of s. typhimurium sr-11, was shown to be avirulent in swine. s. typhimurium chi 4064 was used as a carrier for plasmid pba31-r7, which codes for the expression of a 31-kda protein from brucella abortus (bcsp31). given orally, s. typhimurium chi 4064(pba31-r7) colonized the intestine and mesenteric lymph nodes of 5- to 6-week-old crossbred swine. orally immunized animals developed serum and intestinal antibody responses to ... | 1991 | 1908827 |
| a serological comparison of complement fixation reactions using brucella abortus and b. melitensis antigens in b. abortus infected cattle. | brucella abortus and b. melitensis antigens were used in parallel on the national standard brucella abortus antiserum and on field sera coming from cattle where practically exclusively b. abortus biotypes 1 and 2 have been isolated over the last 11 years. with the national standard serum the titres to b. melitensis were consistently lower than those to b. abortus antigen. most were 1 dilution (twofold) lower. although a similar trend was seen with the field sera, there were 7/346 sera which had ... | 1991 | 1908965 |
| sds-soluble and peptidoglycan-bound proteins in the outer membrane-peptidoglycan complex of brucella abortus. | outer membrane-peptidoglycan complex from brucella abortus was separated from cytoplasmic membrane and cytosol by either sucrose density gradient fractionation or differential (rate) centrifugation of surface labeled cells disrupted by sonication without the use of detergents. the outer membrane-peptidoglycan complex had a buoyant density of 1.22 gm/ml and contained 67 labeled sds-soluble proteins when examined by sds-page. included were four major bands exhibiting molecular masses of 88k, 40k, ... | 1991 | 1909068 |
| effects of gamma radiation and azathioprine on brucella abortus infection in balb/c mice. | sublethal irradiation of balb/c mice 4 hours prior to inoculation with 5 x 10(4) virulent brucella abortus, caused significant (p less than 0.01) reductions in bacterial numbers in comparison with numbers in unirradiated controls. numbers of brucellae in the spleen were significantly lower by 5 days after inoculation and decreased thereafter, so that at 2 and 3 weeks after inoculation, there were up to 1,000-fold fewer organisms in the spleen of irradiated mice. the number of brucellae in the sp ... | 1991 | 1909103 |
| effect of milk stasis on brucella abortus infection of the mammary gland in goats. | to compare the effects of milk stasis and milk flow on brucella abortus infection of the mammary gland under the same systemic conditions, primiparous goats (n = 5) were inoculated iv with b abortus on the day of parturition, and suckling by their neonates was restricted to one mammary gland. goats were euthanatized and necropsied at 3 weeks after inoculation, and milk, mammary glands, and supramammary lymph nodes were evaluated by bacteriologic, histologic, and immunoenzymatic staining techniqu ... | 1991 | 1909104 |
| post natal ontogeny of immunological responsiveness in merino sheep. | sheep in five age groups (two weeks, 10 weeks, 18 weeks, six months and four years old) were immunised systemically, twice, with ovalbumin or brucella abortus (live or killed) and antibody responses in blood were measured. the animals were also infected with the nematode parasites trichostrongylus colubriformis and haemonchus contortus and faecal egg counts and serum antibody responses to larval antigens were measured. the experiments were designed so that, as far as possible, the effect of age ... | 1991 | 1910202 |
| induction of ly-6a/e expression by murine lymphocytes after in vivo immunization is strictly dependent upon the action of ifn-alpha/beta and/or ifn-gamma. | ly-6a/e is a phosphatidylinositol-linked membrane protein which mediates murine t and b cell signalling. ifn-gamma, ifb-alpha/beta, lps, and il-4 have all been reported to induce or upregulate ly-6a/e by normal lymphocytes. since no systematic study has addressed the stimulant selectivity of ly-6a/e expression by murine lymphocytes nor investigated its induction and regulation during primary in vivo immune responses we analyzed in vitro ly-6a/e expression after murine stimuli and during a number ... | 1991 | 1931812 |
| deletion of the bcsp31 gene of brucella abortus by replacement. | the 31-kda salt-extractable immunogenic protein, bcsp31, was deleted from several brucella abortus strains by replacement with a marker gene encoding resistance to the antibiotics kanamycin and neomycin. the bcsp31 gene replacement plasmids, constructed with cole1-derived vectors, were introduced by electroporation into b. abortus strain 19 (s19), into a rough variant of b. abortus s19, and into b. abortus s2308, and antibiotic-resistant transformants were isolated. b. abortus s19 is an attenuat ... | 1991 | 1937745 |
| comparison of results from five serologic methods used for detecting brucella abortus antibody activity in coyote sera. | a total of 423 serum samples representing 94 coyotes which were wild trapped in east texas were used to compare the serologic results from five different methods for detecting antibodies to brucella abortus. the sera were tested for brucella spp. antibody activity by the card (card), rivanol precipitation (riv), standard agglutination tube (sat), cold complement fixation test (cf), and enzyme linked immunosorbent assay (elisa) methods. each serum sample selected for this comparison demonstrated ... | 1991 | 1949585 |
| survival of smooth, rough and transposon mutant strains of brucella abortus in bovine mammary macrophages. | transposon mutants offer a unique way to evaluate the role of lipopolysaccharide (lps) by producing a theoretical single-gene difference between the original strain and the transposon mutant strain. comparative survival of brucella abortus smooth strain 2308, rough rb51, smooth strain 19, and two transposon mutant strains (rough strain 2308::tn5 lac z [m106] and rough strain 19::tn5 lac z [m3], was tested in restrictive bovine mammary macrophages that were able to effectively reduce the percenta ... | 1990 | 1963714 |
| electroporation of a suicide plasmid bearing a transposon into brucella abortus. | the creation of bacterial mutants by transposon mutagenesis has facilitated the identification of regulatory and structural genes. in the case of b. abortus the number of reported transposon mutants created by mating or p1 infection has been relatively small. we studied the conditions necessary to introduce tn5 bearing a kanamycin resistance gene (knr) into b. abortus by electroporation. the highest frequency of tn5 transposition was obtained using b. abortus 2308 harvested at a density of 5.2 x ... | 1990 | 1965986 |
| heterogeneity of mouse helper t cells. evidence from bulk cultures and limiting dilution cloning for precursors of th1 and th2 cells. | many long term mouse th clones express either the type 1 or type 2 th cell (th1 or th2) cytokine secretion phenotype. in this report we present two lines of evidence for the existence of additional th differentiation states. lectin-stimulated spleen cells secreted moderate levels of il-2 compared with long term th1 clones, whereas the levels of other cytokines were more than 100-fold lower than those produced by either th1 or th2 clones. this suggests that many spleen cells produce substantial a ... | 1990 | 1968485 |
| a peripheral mechanism preserves self-tolerance to a secreted protein in transgenic mice. | we have examined mechanisms of tolerance to circulating self-proteins in mice that are transgenic for human insulin. normal, nontransgenic mice develop serum antibody responses when injected with human insulin in cfa; syngeneic transgenic mice do not. b cell responsiveness was assessed by immunizing with human insulin coupled to a t-independent ag, brucella abortus. no differences were found in the numbers of insulin-specific splenic plaque-forming cells between transgenic and nontransgenic mice ... | 1990 | 1974565 |
| genetic variation at the omp2 porin locus of the brucellae: species-specific markers. | the omp2 locus of brucella abortus is composed of two closely related genes (omp2a and omp2b) that encode, and potentially both express, homologous porin proteins. genetic variation at this locus is revealed in the form of restriction-fragment-length polymorphisms which can be used to distinguish the type strains of all six brucella species. five of the six species contain single copies of omp2a and omp2b, whereas brucella ovis appears to have two copies of the omp2a gene. the implications of th ... | 1990 | 1978222 |
| the repertoire diversity and magnitude of antibody responses to bacterial antigens in aged mice: i. age-associated changes in antibody responses differ according to the mouse strain. | aging influences the host immune responses in various ways. in aging mice we have studied the antibody responses to two unrelated bacterial antigens. streptococcus pneumoniae r36a vaccine (pn) and tnp coupled to brucella abortus (tnp-ba). aged animals (20-24 months old) of the c57bl/6 strain had markedly reduced numbers of igm antibody plaque-forming cells (pfc) to pn as compared to young/adult mice (2-3 months old). in contrast, the anti-pn igm pfc responses of aged balb/c mice were consistentl ... | 1991 | 1991330 |
| prophylaxis or treatment of experimental brucellosis with interleukin-1. | intravenously injected recombinant human interleukin-1 alpha (il-1 alpha) given to mice 4 h before infection with brucella abortus 19 depressed the growth of bacteria in the spleen and liver. however, the same dose (10(5) u) or a 10-fold higher dose was not able to decrease numbers of bacteria when given to chronically infected mice. il-1 injected into normal mice induced a dramatic increase 2 h later in colony-stimulating activity in serum, measured by bone marrow proliferation, and in colony-s ... | 1991 | 2019442 |
| serologic survey for bovine pathogens in free-ranging european bison from poland. | from 1980 to 1983, blood was taken from 60 selected european bison (bison bonasus) in poland. serum samples were tested for the presence of antibodies against brucella abortus, 14 serovars of leptospira interrogans, chlamydia spp., coxiella burnetti; foot and and mouth disease virus, bovine leukemia virus and bovine herpes virus-1. in addition, an attempt was made to isolate bovine herpes virus-1 from the prepuce of selected bulls. serological tests suggested chlamydial infection in 28 bison, su ... | 1991 | 2023316 |
| development of a serologic assay for cysticercosis, using an antigen isolated from taenia spp cyst fluid. | an ammonium sulfate-soluble fraction of taenia hydatigena cyst fluid (thfas) was further evaluated for use in the immunodiagnosis of cysticercosis. analysis of thfas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and protein immunoblot analysis confirmed earlier reports of a highly specific, low molecular weight antigen in this preparation; in contrast, other components of thfas were shown to react nonspecifically. antibodies against the less than 12-kd diagnostic antigen were dete ... | 1991 | 2035923 |
| comparative protection of mice against virulent and attenuated strains of brucella abortus by passive transfer of immune t cells or serum. | passively transferred immune serum provided significantly greater protection to balb/c mice against attenuated brucella abortus 19 than against virulent strain 2308, whether serum donors had been infected with strain 19 or 2308. in contrast, immune t cells conferred better protection upon recipients challenged with the homologous strain of b. abortus. it is hypothesized that strain 2308, but not strain 19, can survive in macrophages after opsonization and that epitopes which induce protective ce ... | 1990 | 2104599 |
| evaluation of serologic and cellular immune responses of cattle to a nonlipopolysaccharide antigen from brucella abortus. | cows naturally infected with brucella abortus developed antibody (ab) responses to a nonlipopolysaccharide antigen (nla) purified from b abortus strain 1119-3. sera from strain 19-vaccinated cows did not have detectable amounts of ab. weak lymphoproliferative responses to nla were observed in blood mononuclear cell suspensions obtained from infected cows. there was no evidence of nla-specific lymphoproliferation in cell suspensions from healthy cows. nonlipopolysaccharide antigen binding to bovi ... | 1990 | 2105681 |
| a protein isolated from brucella abortus is a cu-zn superoxide dismutase. | brucella abortus contains a protein that elicits an antigenic response in cattle previously exposed to the organism. the amino acid sequence of the recombinant form of this antigenic protein was determined by gas-phase sequencing of the pyridylethylated protein and its peptides obtained by digestion with cyanogen bromide (cnbr), clostripain, and staphylococcus aureus v8 protease. the brucella protein demonstrated 53.6% identity with the cu-zn superoxide dismutase (sod) from photobacterium leiogn ... | 1990 | 2105741 |
| brucella abortus infection in 14 farm dogs. | fourteen dogs were obtained from 10 farms with brucella-infected cattle and were studied for periods ranging from 2 to 81 days. at necropsy, brucella abortus biovar 4 was isolated from all 14 dogs. mandibular, medial retropharyngeal, tracheobronchial, and mesenteric lymph nodes yielded the highest rate of recovery. urogenital infection with active shedding was seen in a single aged bitch. fecal samples (291 from 13 dogs) were b abortus culture negative. ten dogs monitored serologically over time ... | 1990 | 2107157 |
| ability of mononuclear phagocytes from cattle naturally resistant or susceptible to brucellosis to control in vitro intracellular survival of brucella abortus. | the role of bovine mammary macrophages and peripheral blood monocyte-derived macrophages in natural resistance to bovine brucellosis was evaluated. a group of 11 naturally resistant and 10 chronically infected susceptible cows was studied following challenge with brucella abortus. macrophages from a greater proportion (p less than 0.026) of naturally resistant cows were significantly superior to macrophages from susceptible cows in their ability to inhibit the in vitro intracellular replication ... | 1990 | 2108089 |
| bovine t-lymphocyte lines reactive with brucella abortus. | bovine t-cell lines reactive with brucella abortus were established by repeated stimulation with b abortus and mitomycin c-treated autologous antigen-presenting cells. representative results were obtained, using 33 cell lines from 14 cows. cultures responded to the virulent laboratory strain 2308, the vaccine strain 19, and the rough mutant strain rb51 in thymidine-incorporation assays. the cells in these cultures required antigen-presenting cells for their response to b abortus. autologous anti ... | 1990 | 2109554 |
| immunopathology of experimental brucella abortus strain 19 infection of the genitalia of bulls. | antibody responses in serum and semen, and immunoglobulin containing cell (icc) populations in the genitalia of bulls were compared after inoculating brucella abortus strain 19 into the seminal vesicles of two bulls (isv route) and into testes in two other bulls (it route). bulls seroconverted as early as 1 week post-infection (pi). peak serum titres as determined by the serum agglutination test (sat), complement fixation test (cft) and elisa occurred at pi weeks 3, 4 and 5 respectively. highest ... | 1990 | 2111057 |
| the serological response of cattle immunized with yersinia enterocolitica o:9 or o:16 to yersinia and brucella abortus antigens in enzyme immunoassays. | sera from calves immunized with yersinia enterocolitica serotypes o:9 or o:16 were tested by indirect enzyme linked immunosorbent assay (elisa) using lipopolysaccharide (lps) preparations from brucella abortus or y. enterocolitica o:9 or o:16 for their antibody content of the igg1 or igg2 subclasses. high igg1 responses were present with the three antigens in both groups although some individual variations between animals were noted. the igg2 responses were modest and in some cases not above bac ... | 1990 | 2111058 |
| protection of mice against brucella abortus by immunization with polyclonal anti-idiotype antibodies. | polyclonal goat anti-idiotypic antibodies containing internal images which mimic brucella abortus antigens were generated from rabbit polyclonal idiotypes specific for partially purified extract of b. abortus (px iii). the anti-idiotypic antibodies were purified using two-step immunoaffinity column chromatography. the presence of internal images was demonstrated by competitive inhibition analysis using an enzyme-linked immunosorbent assay. several groups of balb/c mice were vaccinated with the a ... | 1990 | 2111796 |
| immunogenicity of subcellular fractions of brucella abortus: measurement by in vitro lymphocyte proliferative responses. | five groups of heifers were immunized with various subcellular fractions of brucella abortus and tested for their responsiveness in lymphocyte proliferative responses in vitro. the five subcellular fractions used as immunogens were: (1) a mixture of recombinant outer membrane proteins fused to escherichia coli beta-galactosidase, (2) a mixture of outer membrane proteins baomi, baomiib1, and baomiii1, (3) a mixture of outer membrane proteins 7.5 kda and 8.8 kda, (4) a complex of smooth lipopolysa ... | 1990 | 2112287 |
| brucella abortus 16s rrna and lipid a reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class proteobacteria. | on the basis of ribosomal 16s sequence comparison, brucella abortus has been found to be a member of the alpha-2 subdivision of the class proteobacteria (formerly named purple photosynthetic bacteria and their nonphototrophic relatives). within the alpha-2 subgroup, brucellae are specifically related to rickettsiae, agrobacteria, and rhizobiae, organisms that also have the faculty or the obligation of living in close association to eucaryotic cells. the composition of brucella lipid a suggests a ... | 1990 | 2113907 |
| primary antibody responses to thymus-independent antigens in the lungs and hilar lymph nodes of mice. | b lymphocytes from the pulmonary lymphoid tissues were stimulated with a variety of thymus-independent (ti) antigens by intratracheal (i.t.) immunization. immune responses in the lungs and hilar lymph nodes (hln), which are part of the localized lymphoid tissue, as well as in the spleen, the systemic lymphoid organ, were studied. thus, primary i.t. immunization of mice with the ti-1 antigen trinitrophenyl-lipopolysaccharide (tnp-lps) elicited both antigen-specific and polyclonal plaque-forming c ... | 1990 | 2114356 |
| oral immunization of mice with attenuated salmonella typhimurium containing a recombinant plasmid which codes for production of a 31-kilodalton protein of brucella abortus. | salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of s. typhimurium sr-11, was used as a carrier for the plasmid pba31-r7. this plasmid codes for the expression of a 31-kilodalton (kda) protein from brucella abortus (bcsp31). recombinant s. typhimurium chi 4064(pba31-r7) expressed bcsp31 in vitro as shown by western blot (immunoblot) analysis. the plasmid was stable in vitro and in vivo and did not affect the ability of the mutant to invade and colonize the small intestin ... | 1990 | 2114357 |
| penetration and intracellular growth of brucella abortus in nonphagocytic cells in vitro. | in pregnant ruminants, brucella abortus localizes and replicates within the rough endoplasmic reticulum of trophoblastic epithelial cells. in this study, vero cells were exposed to b. abortus to investigate its internalization and intracellular growth in nonphagocytic cells. a new double-fluorescence staining procedure to discriminate between extracellular and intracellular bacteria was developed. studies with the double-fluorescence staining procedure and quantitative bacteriologic culture of d ... | 1990 | 2114362 |
| buffered charcoal-yeast extract medium for the isolation of brucellae. | a patient with chronic osteomyelitis caused by brucella abortus had negative agglutination titers. because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (bcye) agar. sixteen strains of various brucella species were inoculated on bcye agar; selective bcye agar with polymyxin, anisomycin, and cefamandole; and brucella blood agar. the growth and recovery r ... | 1990 | 2116452 |
| brucella abortus in captive bison. i. serology, bacteriology, pathogenesis, and transmission to cattle. | two groups of six, non-brucellosis vaccinated, brucellosis seronegative pregnant american bison (bison bison) were individually challenged with 1 x 10(7) colony forming units (cfu) of brucella abortus strain 2308. three days after challenge, each bison group was placed in a common paddock with six non-vaccinated, brucellosis susceptible, pregnant domestic heifers. in a parallel study, two groups of six susceptible, pregnant cattle were simultaneously challenged with the identical dose as the bis ... | 1990 | 2117675 |
| immunogenicity of brucella-extracted and recombinant protein vaccines in cd-1 and balb/c mice. | a study was conducted to determine whether subcomponent proteins (previously identified as bcsp20, bcsp31, and bcsp45, and the corresponding recombinant proteins rbcsp20, rbcsp31, and rbcsp45) that were recovered from the cell surface of brucella abortus strain 19 were immunogenic and protective for mice when compared with brucella cell surface protein (bcsp) and with a proteinase k-treated lipopolysaccharide (pklps) extracted from b abortus strain 2308. protection was evaluated after challenge ... | 1990 | 2118744 |
| identification of the bacterium associated with haemorrhagic septicaemia in rainbow trout as hafnia alvei. | an unidentified bacterium responsible for epizootic haemorrhagic septicaemic disease in rainbow trout was recently described. this organism exhibited some antigenic similarity to brucella abortus and to yersinia ruckeri and was presumed to belong to the family enterobacteriaceae. further biochemical characterization and dna relatedness studies showed that pathogenic fish strains are hafnia alvei. | 1990 | 2120748 |
| antibody responses to thymic independent antigens in the peripheral and mesenteric lymph nodes of mice. | differentiation of b lymphocytes from various peripheral lymph nodes (pln) of mice was studied in the in vitro and in vivo conditions. subcutaneous (sc) immunization in the foot pads with thymic independent (ti)-2 antigens such as polyvinyl pyrrolidone (pvp), r36a, and trinitrophenyl (tnp)-dextran failed to elicit plaque forming cell (pfc) responses from the draining popliteal and inguinal lymph nodes, whereas the splenic b cells of these animals exhibited good pfc response. injection of unconju ... | 1990 | 2121216 |
| case report and seeded blood culture study of brucella bacteremia. | diagnosis of brucellosis requires prompt detection and identification of the coccobacillus for appropriate patient management, as the organism is associated with a potentially severe outcome. in a recent experience, an 18-year-old migrant farm worker presented at a local hospital with nonspecific symptoms. a significant brucella titer of 2,560 was followed by the recovery of a gram-negative coccobacillus, subsequently identified as brucella abortus, from subcultured 5-day-old bactec nr730 negati ... | 1990 | 2121795 |
| [reaction of the injured organism to infection]. | authors have examined degree of the immune response to experimentally inoculated agent in the early (6 hours) and late (7 days) phase of the organism response to thermal injury in balb/c rats divided into two experimental groups and three control groups (only infected, only burnt and intact). brucella abortus bovis of va-19 species known as a good immune response stimulator in the host was i.p. inoculated in ld 50 (1 x 10(7) bact/ml). as the infective agent. thermal injury-scalding was induced a ... | 1990 | 2122591 |
| effect of dose of brucella abortus strain 19 in yearling heifers on the relative risk of developing brucellosis from challenge exposure with strain 2308. | yearling heifers were given sc injections of 10(8) (n = 40), 10(9) (n = 44), or 10(10) (n = 44) colony-forming units of brucella abortus strain 19 (s19). the proportion of heifers with positive serologic test results at 1 month following vaccination increased as the dose of s19 increased. these proportions decreased with time, and all heifers had negative card, rivanol, and complement fixation test results within 4 months. positive elisa results persisted beyond 4 months in all three s19 dose gr ... | 1990 | 2122782 |
| zoonotic infections in northern ireland farmers. | evidence of past zoonotic infection was investigated serologically in randomly selected northern ireland farmers. the percentage of farmers with antibody was: brucella abortus (0.7), leptospira interrogans serovars (8.1), borrelia burgdorferi (14.3), toxoplasma gondii (73.5), coxiella burnetii (28.0), chlamydia psittaci (11.1) and hantavirus (1.2). the results show that northern ireland farmers have been exposed in the past to zoonotic infections. it is not known if these infections contributed ... | 1990 | 2123457 |
| antibodies to some pathogenic agents in free-living wild species in tanzania. | a total of 535 sera from eight species of wildlife were collected from different game areas in tanzania between 1987 and 1989. these sera were tested for antibodies against foot-and-mouth disease, bovine herpes virus types 1 and 2, lumpy skin disease, bovine viral diarrhoea, akabane, bovine ephemeral fever, bluetongue, enzootic bovine leucosis, african horse sickness and african swine fever viruses and brucella abortus based on the expected species susceptibility. sera from buffalo syncerus caff ... | 1990 | 2123458 |
| induction of lymphocyte responsiveness by the outer membrane-peptidoglycan complex of rough strains of brucella abortus. | the outer membrane-peptidoglycan complex (om-pg) from rough strains of brucella abortus was tested for its ability to induce lymphocyte responsiveness in cattle. six groups of heifers were immunized with varying doses and administration schedules of rough om-pg and assayed for responsiveness of their lymphocytes in proliferation assays in vitro. all om-pg preparations were emulsified in a commercial adjuvant for administration. two other groups of heifers were immunized with strain 19 vaccine or ... | 1990 | 2123582 |
| brucella suis s2, brucella melitensis rev. 1 and brucella abortus s19 living vaccines: residual virulence and immunity induced against three brucella species challenge strains in mice. | live attenuated brucella suis s2 vaccine was compared to living vaccines b. abortus s19 and b. melitensis rev. 1 in mice. residual virulence was estimated by ability to multiply and persist in spleen and lymph nodes. immunogenicity was estimated by spleen counts of control and vaccinated mice challenged either with the reference b. abortus 544 strain or with virulent b. melitensis h38 and b. suis 1330 strains. s2 vaccine had lower residual virulence; expressed as 50% recovery time, persistence w ... | 1990 | 2123586 |
| effect of tannic acid on the immune response of growing chickens. | effects of tannic acid (ta) on the immune response of growing chickens were studied in two experiments. in the first experiment, 25 or 30 g ta/kg diet was added to a control diet; added ta reduced (p less than .05) weight gain, protein efficiency rate and weight of bursa of fabricius, thymus and spleen. total igm and igg immunoglobulin levels (primary and secondary immune responses) against killed brucella abortus were reduced in ta-fed chickens. numbers of total white blood cells and absolute l ... | 1990 | 2123848 |
| comparative histopathology in balb/c mice infected with virulent and attenuated strains of brucella abortus. | the course of infection in balb/c mice of virulent brucella abortus strain 2308 (s-2308) and attenuated strain 19 (s-19) varies markedly. whereas s-19 is eliminated at an exponential rate beginning at 2 weeks post infection (p.i.), strain 2308 assumes a steady state or plateau during the first 6 weeks p.i. and thereafter is eliminated very slowly over a period exceeding 6 months. here we compared the initiation and maintenance of inflammatory reactions in spleens and livers of mice infected with ... | 1990 | 2124401 |
| [detection of antibodies against brucella in the aqueous humor using elisa]. | in aqueous bulbi of experimentally against brucella abortus immunized guinea pigs and rabbits specific antibodies could be detected by elisa-tests. this first demonstration of brucella antibodies in the aqueous shows that aqueous can be used for serological examinations. for practical use more investigations in experimental animals are necessary, e.g. on kinetics of orbital antibodies absence of interfering factors and biomechanics of proteins in aqueous. | 1990 | 2125201 |
| the persistence of serological reactions following heifer vaccination with brucella abortus strain 19 on brucellosis-free farms. | of 392 heifers vaccinated between 4 and 9.5 months of age and tested at 22 months or older, 3 (0.77%) had titres in the complement fixation test of between 30 and 98 international units per millilitre. the results confirm that persistent serological reactions can be a problem even in brucellosis-free herds. to reduce the incidence of these problem reactors, it is suggested that: in clean herds the age of inoculation be reduced to 3 months of age; the age at first testing be delayed as long as po ... | 1990 | 2125318 |
| [increasing the effectiveness of antibiotic therapy by correcting immunologic disorders with vitamin a in patients with brucellosis]. | experimental studies and clinical trials were performed on possible increase of antibiotic therapy efficacy in brucellosis patients by correction of the immunity disorders with vitamin a. it was experimentally shown that vitamin a increased cellular immunity and accelerated sanation of guinea pigs sensitized with brucella abortus 19 ba. the clinical trials demonstrated that the use of vitamin a in a dose of 33,000 iu thrice a day for 10 to 12 days during the complex treatment of patients with ac ... | 1990 | 2126706 |
| effect of vitamin e and selenium supplementation on some immune parameters following vaccination against brucellosis in cattle. | twenty-four 7-mo-old beef heifers (charolais simmental cross), weighing 213 kg, were used to determine the effect of vitamin e (vite) and(or) selenium (se) supplementation on the humoral response to a standard dose of brucella abortus strain 19 vaccine and on the levels of naturally occurring immunoglobulins (ig) to several antigens. the treatments were as follows: group 1, no supplement; group 2, supplementation with 6 g of elemental se; group 3, supplementation with 1,400 iu/d of vite; and gro ... | 1990 | 2126787 |
| persistent infection with brucella abortus biotype 1 in a cow. | | 1990 | 2126804 |
| immunomodulation in cattle immunized with brucella abortus strain 19. | regulation of the bovine immune response to immunization with brucella abortus strain 19 (s19) was investigated through application of a modification of an assay to measure suppressor t lymphocyte activities in humans and through development and characterization of antigen-stimulated t lymphocyte lines in vitro. a total of nine of steers were alloted into two groups: control (n = 4) and s19-immunized (n = 5). peripheral blood mononuclear cells (pbmc) from each animal were cultured in vitro with ... | 1990 | 2127487 |
| brain abscess caused by brucella abortus and staphylococcus aureus in a child. | a case of a 12-year-old male with fallot's tetralogy and brain abscess due to brucella and staphylococcus is presented. the abscess was aspirated and brucella abortus and staphylococcus aureus were isolated. | 1990 | 2127588 |
| enzyme immunoassay using mouse monoclonal anti-bovine antibodies for the detection of brucella abortus antibodies in cow milk. | individual milk samples and artificially constructed tank milk samples from cows with naturally occurring brucellosis were examined by the enzyme-linked immunosorbent assay (elisa) using sonicated b. abortus s-99 antigen, and mouse monoclonal anti-bovine igm, iga, and igg1 conjugates. elisa results were compared with the results of the milk ring test using either 1 ml milk (mrt-1) or 8 ml milk (mrt-8). the elisa using mouse monoclonal anti-bovine igg1 conjugate was sensitive and specific. in tes ... | 1990 | 2127977 |
| serological survey of brucella abortus antibody prevalence in the one-humped camel (camelus dromedarius) from eastern sudan. | a five year investigation of brucella antibody prevalence in camel sera was conducted in 1502 one-humped camels of both sexes and different ages. the average (mean +/- sd) incidence rate of positive results was 6.95 +/- 1.55%. among adult one-humped camels, the rate was 4.94 +/- 2.51% in males and 13.76 +/- 4.41% in females. juvenile one-humped camel calves showed a 0% incidence rate in males and a 1.82 +/- 3.64% in females. antibodies against brucella abortus were prevalent in one-humped camel ... | 1990 | 2128713 |
| identification of brucella abortus strain 19 by decreased ability to utilize erythritol as determined by gas liquid chromatography. | a method to identify brucella abortus strain 19 by erythritol utilization using gas liquid chromatography (glc) was developed. a total of 69 strains of b. abortus (41 virulent field strain isolates and 28 strain 19 isolates) were tested. following incubation of the isolate with a standard amount of erythritol, the erythritol present in the cell suspension was acetylated and measured by glc. field strains of b. abortus utilized an average of 90.9% of the erythritol, whereas vaccine strains utiliz ... | 1990 | 2128815 |
| immunosuppression by peritoneal bcg-induced granulomas in mice. | 1. intraperitoneal injection of viable bcg resulted in the formation of peritoneal granulomas in mice. granuloma-bearing animals presented a specific immunosuppression of delayed-type hypersensitivity (dth) to bcg. 2. the transplantation of peritoneal granulomas induced by bcg to animals previously sensitized with bcg suppressed the dth response to this antigen in the recipient animals to the same extent as observed in animals with granulomas induced by the injection of bcg. no suppression was o ... | 1990 | 2128819 |