| subgingival microbiota of chilean patients with chronic periodontitis. | an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago. | 2004 | 15212354 |
| implant therapy following liver transplantation: clinical and microbiological results after 10 years. | the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ... | 2004 | 15295960 |
| cloning and expression of afpa, a gene encoding an antifreeze protein from the arctic plant growth-promoting rhizobacterium pseudomonas putida gr12-2. | the arctic plant growth-promoting rhizobacterium pseudomonas putida gr12-2 secretes an antifreeze protein (afp) that promotes survival at subzero temperatures. the afp is unusual in that it also exhibits a low level of ice nucleation activity. a dna fragment with an open reading frame encoding 473 amino acids was cloned by pcr and inverse pcr using primers designed from partial amino acid sequences of the isolated afp. the predicted gene product, afpa, had a molecular mass of 47.3 kda, a pi of 3 ... | 2004 | 15317770 |
| microbiological profile of early onset/aggressive periodontitis patients. | the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence. | 2004 | 15327644 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| relationship of subgingival and salivary microbiota to gingival overgrowth in heart transplant patients following cyclosporin a therapy. | severe gingival overgrowth (go) is induced in patients taking cyclosporin a (csa) following organ transplantation. determining which patient will develop go is still not possible. the purpose of this study was to establish an association between csa and gingival overgrowth in heart transplant patients taking into account periodontal and microbiological conditions. | 2004 | 15341348 |
| possible periodontal pathogens associated with clinical symptoms of periodontal disease in japanese high school students. | the purpose of this study is to investigate how the components of biofilm and clinical oral status change in adolescents and to identify specific periodontal pathogens as risk markers for the onset of periodontitis. | 2004 | 15455735 |
| need for procedural details in detection of periodontopathic bacterial dna in the atheromatous plaque by pcr. | | 2004 | 15472382 |
| the distribution of periodontopathic bacteria among japanese children and their parents. | it is not well known how periodontopathic bacteria colonize in the oral cavity during childhood. the purpose of this study was to investigate the distribution of periodontopathic bacteria in oral cavities of children and their parents and the relationship between the bacterial findings and clinical parameters. | 2004 | 15491344 |
| characterization of bacterial community diversity in cystic fibrosis lung infections by use of 16s ribosomal dna terminal restriction fragment length polymorphism profiling. | progressive loss of lung function resulting from the inflammatory response to bacterial colonization is the leading cause of mortality in cystic fibrosis (cf) patients. a greater understanding of these bacterial infections is needed to improve lung disease management. as culture-based diagnoses are associated with fundamental drawbacks, we used terminal restriction fragment (t-rf) length polymorphism profiling and 16s rrna clone data to characterize, without prior cultivation, the bacterial comm ... | 2004 | 15528712 |
| herpesviruses, the missing link between gingivitis and periodontitis? | herpesviruses appear to assume a major etiopathogenic role in various types of destructive periodontal disease. human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hcmv-ebv co-infection are closely associated with disease-active periodontitis in juveniles and adults, with acute necrotizing ulcerative gingivitis in children, and with periodontal abscesses. in particular, hcmv reactivation in periodontitis lesions seems to be linked to advancing disease. hcmv infects periodontal monocytes/m ... | 2004 | 15553977 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| fusobacterium nucleatum increases collagenase 3 production and migration of epithelial cells. | fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. initiation and outcome of infective diseases depend critically on the host cell signaling system altered by the microbe. production of proteinases by infected cells is an important factor in pericellular tissue destruction and cell migration. we studied binding of f. nucleatum to human epit ... | 2005 | 15664960 |
| periodontal infections and pre-term low birth weight: a case-control study. | pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ... | 2005 | 15691348 |
| impact of sample storage on detection of periodontal bacteria. | information on the impact of sample storage prior to analysis by dna methods is limited. the aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration. | 2005 | 15720575 |
| phototargeting oral black-pigmented bacteria. | we have found that broadband light (380 to 520 nm) rapidly and selectively kills oral black-pigmented bacteria (bpb) in pure cultures and in dental plaque samples obtained from human subjects with chronic periodontitis. we hypothesize that this killing effect is a result of light excitation of their endogenous porphyrins. cultures of prevotella intermedia and p. nigrescens were killed by 4.2 j/cm2, whereas p. melaninogenica required 21 j/cm2. exposure to light with a fluence of 42 j/cm2 produced ... | 2005 | 15793117 |
| dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study. | currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ. | 2005 | 15830645 |
| severe periodontitis in a 5-year-old girl with hyperimmunoglobulin e syndrome. | the hyperimmunoglobulin e syndrome (hies) is a multisystem disorder that affects the: (1) dentition; (2) skeleton; (3) connective tissues; and (4) immune system. little is known about periodontal manifestations of the syndrome. the purpose of this report was to describe a 5-year-old girl with suspected autosomal-recessive hies syndrome who revealed profusely bleeding and painful gingiva and generalized aggressive periodontitis. a polymerase chain reaction (pcr)-based microbiological examination ... | 2005 | 15839398 |
| campylobacter rectus mediates growth restriction in pregnant mice. | recent studies have suggested that subclinical infection may be an important cause of low birth weight. campylobacters are important human pathogens, causing septicemia and occasionally abortion, premature labor, or severe perinatal infection. the potential role of oral species of campylobacter in mediating adverse pregnancy outcomes in animal models has not yet been determined. our objective was to determine the effects of campylobacter rectus (c. rectus) infection on pregnancy outcomes in a mo ... | 2005 | 15857095 |
| oral campylobacter species involved in extraoral abscess: a report of three cases. | oral campylobacter species are rarely reported to cause extraoral infections. here we present three cases of extraoral abscess caused by an oral campylobacter sp. and a streptococcus sp. the campylobacter species were all isolated anaerobically and identified by sequencing analysis of the 16s rrna gene. the cases included a breast abscess caused by campylobacter rectus and a non-group a beta-hemolytic streptococcus in a patient with lymphoma, a liver abscess caused by campylobacter curvus and an ... | 2005 | 15872299 |
| tobacco smoking and periodontal microflora in a saudi arabian population. | to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits. | 2005 | 15882210 |
| associations between igg antibody to oral organisms and carotid intima-medial thickness in community-dwelling adults. | the aims of this study are to describe the relationships between igg antibodies to 17 oral organisms and atherosclerosis as indexed by carotid intima-medial wall thickness (imt) and to evaluate the role of smoking. | 2005 | 15893320 |
| identification of periodontal pathogens in atherosclerotic vessels. | epidemiological studies have shown that periodontitis may be associated with presence of atherosclerosis. dna from periodontal pathogens has been detected in atherosclerotic lesions, but viable oral bacteria have not yet been isolated from atherosclerotic plaques. the purpose of the present study was to determine if viable oral bacteria could be isolated from atherosclerotic lesions and if dna from periodontal pathogens could be detected by use of polymerase chain reaction (pcr) techniques. | 2005 | 15898933 |
| effect of female sex hormones on campylobacter rectus and human gingival fibroblasts. | recent studies have suggested a relationship between maternal campylobacter rectus infections and preterm low birth weight. the purpose of this study was to investigate the effect of female sex hormones, estradiol and progesterone, on c. rectus and human gingival fibroblasts (hgf). the growth of c. rectus was significantly enhanced by incorporating either estradiol or progesterone in the culture medium. the production of vascular endothelial growth factor (vegf), interleukin (il)-6 and il-8 by h ... | 2005 | 15943769 |
| the salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects. | the purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (oscc) lesion would differ from those found in cancer-free (oscc-free) controls. | 2005 | 15987522 |
| subgingival distribution of campylobacter rectus and tannerella forsythensis in healthy children with primary dentition. | it is important to know how many subgingival plaque samples should be assayed from a child to ascertain infection with a periodontal pathogen. plaque samples from several sites may fail to detect some important bacteria if only a limited number of gingival sites are sampled. the purpose of this study was to evaluate the detection of periodontal pathogens in a large number of subgingival sites in the same children in order to determine the number of samples necessary. | 2006 | 15990083 |
| oral bacteria in the occluded arteries of patients with buerger disease. | recent studies have suggested that infectious organisms play a role in vascular diseases. in this study, to explore a possible link between oral infection and buerger disease, we investigated whether oral (periodontal) bacteria were present in occluded arteries removed from patients with characteristic buerger disease. | 2005 | 16012459 |
| campylobacter surface-layers (s-layers) and immune evasion. | many pathogenic bacteria have evolved mechanisms for evading host immune systems. one evasion mechanism is manifest by the surface layer (s-layer), a paracrystalline protein structure composed of s-layer proteins (slps). the s-layer, possessed by 2 campylobacter species (c. fetus and c. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. c. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstre ... | 2002 | 16013216 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. | accurate laboratory tests for the detection and quantification of periodontopathogens in subgingival plaque samples of periodontal disease patients are becoming essential to study the pathogenesis of this polymicrobial condition. we used a real-time polymerase chain reaction (pcr) assay for the quantification of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, dialister pneumosintes, campylobacter rectus, and micromonas micros as well as total eubacteria in ... | 2005 | 16171445 |
| distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers. | we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ... | 2006 | 16269128 |
| effects of maternal campylobacter rectus infection on murine placenta, fetal and neonatal survival, and brain development. | maternal periodontal infection has been associated with increased risk of prematurity and low birthweight. infection and inflammatory pathways that mediate prematurity have also been implicated in neonatal developmental impairments. the objective of this study was to determine whether maternal campylobacter rectus infection that induces fetal growth restriction in a mouse model also compromises neonatal pup survival, growth, and neurodevelopment. | 2005 | 16277586 |
| immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control. | to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions. | 2006 | 16367855 |
| detection of cfxa and cfxa2, the beta-lactamase genes of prevotella spp., in clinical samples from dentoalveolar infection by real-time pcr. | while most bacteria involved in dentoalveolar infection are highly susceptible to penicillin, some prevotella strains exhibit resistance to this agent through the production of beta-lactamase. the production of beta-lactamase by prevotella spp. is in turn associated with the expression of the genes cfxa and cfxa2. the aim of the present study was to determine the prevalence of cfxa and cfxa2 in prevotella strains by use of real-time pcr and to assess the performance of this molecular method for ... | 2006 | 16390966 |
| subgingival and tongue microbiota during early periodontitis. | periodontal infections have a microbial etiology. association of species with early disease would be useful in determining which microbes initiate periodontitis. we hypothesized that the microbiota of subgingival and tongue samples would differ between early periodontitis and health. a cross-sectional evaluation of 141 healthy and early periodontitis adults was performed with the use of oligonucleotide probes and pcr. most species differed in associations with sample sites; most subgingival spec ... | 2006 | 16567551 |
| identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions. | bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. this study determined the prevalence of a group of oral bacterial species on or in epithelial cells derived from periodontal pockets and the gingival crevice of subjects with periodontitis. samples of epithelial cells were obtained from 120 sites with periodontal pockets > or ... | 2006 | 16585650 |
| supragingival plaque may modify the effects of subgingival bacteria on attachment loss. | bacterial interactions in response to local and systemic factors may explain important features of the pathogenesis of periodontal disease. this study evaluates the role of supragingival plaque level on the relationship between subgingival microorganisms and the risk of attachment loss. | 2006 | 16671872 |
| a model of spontaneous periodontitis in the miniature goat. | the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis. | 2006 | 16671878 |
| [prevalence of putative periodontal microorganisms in chinese patients with aggressive periodontitis]. | to evaluate the prevalence of putative periodontal microorganism in chinese patients with aggressive periodontitis (agp). | 2006 | 16836903 |
| effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes. | patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ... | 2006 | 16841550 |
| periodontal microflora of hiv infected patients with periodontitis. | the aim of this study was to determine the microbial profile of periodontal lesions in hiv seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. the subgingival microflora of 20 cdc ii, 20 cdc iii, 20 cdc iv/v and 20 systemically healthy patients with rapidly progressing periodontitis was examined. four sites with greatest probing depth in each patient were selected for microbiological sampling. the samples were cultured aerobicall ... | 1997 | 16887570 |
| subgingival microflora in smokers with early onset periodontitis. | cigarette smoking is a potent risk factor which has recently been associated with periodontal disease progression. the objective of this study was to detect the microbial profile of early onset periodontitis in smokers and compare it to that of non-smokers. the study population consisted of 50 systemically healthy individuals aged 25 to 38 years, exhibiting early onset periodontitis. 25 patients were smokers (> 20 cigarettes/day) and 25 non-smokers. two pooled bacterial samples comprised of four ... | 1997 | 16887581 |
| salivary immunoglobulin a directed to oral microbial groel in patients with periodontitis and their potential protective role. | the aim of this study was to identify salivary immunoglobulin a (iga) directed to oral microbial groel in patients with periodontitis and to demonstrate their potential protective role through a reduction of inflammatory cytokine production induced by microbial groel. using five different proteins belonging to the heat-shock protein 60 family, western immunoblot analysis of salivary iga from 63 subjects revealed immunoreactivities with campylobacter rectus groel and porphyromonas gingivalis groe ... | 2006 | 16922927 |
| detection of cariogenic streptococcus mutans in extirpated heart valve and atheromatous plaque specimens. | the involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. however, no information is available regarding the involvement of cariogenic pathogens such as streptococcus mutans. the presence of oral streptococcal species and periodontitis-related bacteria in 35 ... | 2006 | 16954266 |
| changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16s cloning and sequencing. | the gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. the objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status u ... | 2006 | 17021095 |
| infection frequency of epstein-barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters. | to detect the infection frequencies of different genotypes of epstein-barr virus (ebv) in subgingival samples from chronic periodontitis (cp) patients, and to discuss the correlation between infection with ebv and clinical parameters. | 2006 | 17048301 |
| rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease. | one of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. in this study we hypothesized that p. gingivalis, t. denticola, and t. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in ... | 2007 | 17210663 |
| interleukin-1alpha stimulation in monocytes by periodontal bacteria: antagonistic effects of porphyromonas gingivalis. | periodontal pathogenic bacteria are associated with elevated levels of interleukin-1alpha (il-1alpha) but it is unclear if all species can induce cytokine production equally. porphyromonas gingivalis may be able antagonize il-1alpha induced by other species through the activity of its proteases or lipopolysaccharide (lps). monomac-6 cells and primary human monocytes were treated with culture supernatants from porphyromonas gingivalis, fusobacterium nucleatum, campylobacter rectus, actinobacillus ... | 2007 | 17241171 |
| women with a recent history of early-onset pre-eclampsia have a worse periodontal condition. | pre-eclampsia is a complication of pregnancy characterized by systemic vascular dysfunction and pathological changes in placental arteries. growing evidence of chronic infection as an aetiological factor in vascular diseases prompted us to study maternal periodontal disease in subjects with early-onset pre-eclampsia (<34 weeks). | 2007 | 17309594 |
| the detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study. | periodontal disease is a commonly prevalent problem faced alike by both the developed and third world countries but showing wide variations in prevalence and severity across different geographical areas. the purpose was to identify actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), ekinella corrodens (ec), campylobacter rectus (cr), bacteroides forsythus (bf), treponema denticola (td) and fusobacterium nucleatum (fn) in indian adult periodontiti ... | 2007 | 17347537 |
| demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in colombia: a multicenter study. | the microbial profile of periodontal disease varies among different human populations. this study evaluated the demographic, clinical, and microbiologic aspects of periodontitis in a multigeographic sample in colombia. | 2007 | 17397309 |
| intrinsic apoptotic pathways of gingival epithelial cells modulated by porphyromonas gingivalis. | porphyromonas gingivalis can inhibit chemically induced apoptosis in primary cultures of gingival epithelial cells through blocking activation of the effector caspase-3. the anti-apoptotic phenotype of p. gingivalis is conserved across strains and does not depend on the presence of fimbriae, as fimbriae-deficient mutants and a naturally occurring non-fimbriated strain were able to impede apoptosis. to dissect the survival pathways modulated by p. gingivalis, protein and gene expression of a numb ... | 2007 | 17419719 |
| herpesviral-bacterial synergy in the pathogenesis of human periodontitis. | periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role. | 2007 | 17471038 |
| bacterial species in subgingival plaque and oral bone loss in postmenopausal women. | oral bacteria are widely recognized in the etiology of periodontal disease. we investigated the prevalence of subgingival bacterial infection with eight species, tested associations between infection and oral bone loss, and assessed potential confounding factors and effect modifiers of those associations in a large community-based cohort of postmenopausal women. | 2007 | 17539719 |
| [subgingival microbiota in chronic and aggressive periodontitis in bogotá, colombia: an epidemiological approach]. | periodontitis-associated microbiotas differ in regions around the world and vary with a variety of factors. this suggests that each country must establish its own dental microbiobiotic profile in order to formulate adequate prevention measures and more specific treatments. | 2007 | 17546221 |
| microbial colonization of the upper gastrointestinal tract in patients with barrett's esophagus. | barrett's esophagus (be) is a complication of chronic gastroesophageal reflux disease, in which patients are at greatly increased risk of esophageal dysplasia and adenocarcinoma. over the past 2 decades, there has been an increase in the incidence of both be and adenocarcinoma; however, the involvement of microorganisms in be is uncertain. the aim of this study was to characterize microbial communities in esophageal aspirate specimens and on distal esophageal mucosal samples from patients with b ... | 2007 | 17554697 |
| population-based study of salivary carriage of periodontal pathogens in adults. | large, general population-based data on carriage rates of periodontal pathogens hardly exist in the current literature. the objectives of the present study were to examine the salivary detection of aggregatibacter (formerly actinobacillus) actinomycetemcomitans, campylobacter rectus, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, and treponema denticola in a representative sample of the adult population living in southern finland and to clarify which determinants are a ... | 2007 | 17567788 |
| relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease. | several reports have demonstrated a possible association of periodontal infections with coronary heart disease (chd) by elevated antibody titre to periodontopathic bacteria in chd patients compared with non-diseased controls. although each periodontopathic bacterium may vary in virulence for periodontitis and atherosclerosis, antibody response to multiple bacteria in chd patients has not been understood fully. therefore, serum levels of antibody to 12 periodontopathic bacteria together with othe ... | 2007 | 17645769 |
| porphyromonas gingivalis antagonises campylobacter rectus induced cytokine production by human monocytes. | porphyromonas gingivalis and campylobacter rectus are two major bacterial species implicated in the pathogenesis of periodontitis. p. gingivalis can antagonise the inflammatory response to other periodontal pathogens, a property commonly attributed to its lipopolysaccharide (lps). the aim of this study was to investigate the capacity of p. gingivalis to antagonise c. rectus induced cytokine stimulation from human monocytes, and to investigate the involvement of its lps. primary human monocytes a ... | 2007 | 17709256 |
| periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: a case-control study. | recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. this case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (cad) based on clinical and periodontal microbiologic parameters. | 2007 | 17760542 |
| correlation between infections with different genotypes of human cytomegalovirus and epstein-barr virus in subgingival samples and periodontal status of patients. | accumulating evidence indicates that herpesviruses may be putative pathogens in various types of periodontal diseases. the present study was performed to examine infections with different genotypes of human cytomegalovirus (hcmv) and epstein-barr virus (ebv) in subgingival samples from a chinese population and to analyze the correlation with periodontal status. a nested pcr assay was used to identify the presence of hcmv, ebv type 1 (ebv-1), and ebv-2; and the amplicons were further analyzed by ... | 2007 | 17804655 |
| implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers. | the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ... | 2007 | 17912965 |
| quantitative proteomics of intracellular porphyromonas gingivalis. | whole-cell quantitative proteomic analyses were conducted to investigate the change from an extracellular to intracellular lifestyle for porphyromonas gingivalis, a gram-negative intracellular pathogen associated with periodontal disease. global protein abundance data for p. gingivalis strain atcc 33277 internalized for 18 h within human gingival epithelial cells and controls exposed to gingival cell culture medium were obtained at sufficient coverage to provide strong evidence that these change ... | 2007 | 17979175 |
| periodontal disease at the biofilm-gingival interface. | a molecular epidemiologic study provided the opportunity to characterize the biology of the biofilm-gingival interface (bgi) in 6,768 community-dwelling subjects. | 2007 | 18062113 |
| antimicrobial susceptibility and beta-lactamase production of anaerobic and aerobic bacteria isolated from pus specimens from orofacial infections. | most suppurative orofacial infections are polymicrobial. information regarding the antimicrobial susceptibility of the microorganisms involved can be useful in the choice of an effective antibiotic therapy. in this study we determined the antimicrobial susceptibility of a total 235 anaerobic and aerobic bacteria recently isolated from pus specimens of orofacial infections. all the viridans streptococci were susceptible to penicillin, cefotaxime, cefoxitin, imipenem and levofloxacin. imipenem and ... | 2007 | 18073147 |
| quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis. | the development of human periodontitis may depend upon cooperative interactions among herpesviruses, specific pathogenic bacteria and tissue-destructive inflammatory mediators. this study sought to identify associations among human cytomegalovirus, epstein-barr virus and six putative periodontopathic bacteria in periodontitis lesions. | 2008 | 18086168 |
| relationship between campylobacter rectus and periodontal status during pregnancy. | in a previous study, we showed that the growth of campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. in the present study, we examined the relationship between c. rectus levels in the saliva and the periodontal status of pregnant women. | 2008 | 18173799 |
| etiological analysis of initial colonization of periodontal pathogens in oral cavity. | it is unclear when the initial colonization by periodontal pathogens occurs in the oral cavity. therefore, we report here the association between specific age groups and the time when the initial colonization by periodontal pathogens occurs in the oral cavity in such groups. findings are based on an epidemiological analysis of the prevalence of five periodontal pathogens in the oral cavities of a wide range of age populations, from newborn to elderly, who were randomly selected in a geographic r ... | 2008 | 18287326 |
| colonization pattern of periodontal bacteria in japanese children and their mothers. | the purpose of this study was to determine the time of infection by anaerobic gram-negative rods associated with periodontal disease, and to clarify their transmission from mother to child. | 2008 | 18302616 |
| microbiologic and immunologic characteristics of periodontal disease in hispanic americans with type 2 diabetes. | the microbiology of periodontitis in type 1 diabetes has been reported, but less is known about type 2 diabetes. moreover, these data have not linked microbial colonization, host response, and clinical presentation in type 1 or type 2 diabetes. the objectives of this study were to relate periodontal status, periodontal microorganisms, and host-response characteristics in hispanic americans with type 2 diabetes. | 2008 | 18380556 |
| bleeding on probing differentially relates to bacterial profiles: the oral infections and vascular disease epidemiology study. | various bacterial species are differentially prevalent in periodontal health, gingivitis or periodontitis. we tested the independent associations between three bacterial groupings and gingival inflammation in an epidemiological study. | 2008 | 18400025 |
| [microbiological and biochemical characteristics of inflammatory tissues in the periodontium]. | to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva. | 2008 | 18413987 |
| effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates. | this report evaluated systemic inflammatory and immune biomarkers in a cohort of macaca mulatta (rhesus monkeys) maintained as a large family social unit, including an age range from <1 year to >24 years. we hypothesized that the systemic host responses would be affected by the age, gender, and clinical oral presentation of the population, each contributing to inflammatory and immune responses that would reflect chronic oral infections. the results demonstrated that the prevalence and severity o ... | 2008 | 18448617 |
| photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. | recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ... | 2009 | 18465191 |
| assessment of the microbiota in root canals of human primary teeth by checkerboard dna-dna hybridization. | the purpose of this study was to evaluate in vivo the prevalence of microorganisms in root canals of human primary teeth, by checkerboard dna-dna hybridization. | 2007 | 18477431 |
| [relationship between herpesviruses and periodontal pathogenic bacteria in subgingival plaque]. | to evaluate the prevalence rates of human cytomegalovirus(hcmv) and epstein-barr virus-1(ebv-1) in subgingival plaque and analyze the relationship between herpesviruses, periodontal pathogenic bacteria and periodontal clinical parameters in chinese patients with aggressive periodontitis(agp). | 2008 | 18560463 |
| rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method. | loop-mediated isothermal amplification (lamp) was applied to develop a rapid and simple detection system for eight periodontal pathogens: aggregatibacter (actinobacillus) actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia. primers were designed from the 16s ribosomal rna gene for each pathogen, and the lamp amplified the targets specifically and efficiently under ... | 2008 | 18565108 |
| differential gender effects of a reduced-calorie diet on systemic inflammatory and immune parameters in nonhuman primates. | dietary manipulation, including caloric restriction, has been shown to impact host response capabilities significantly, particularly in association with aging. this investigation compared systemic inflammatory and immune-response molecules in rhesus monkeys (macaca mulatta). | 2008 | 18565132 |
| biosynthesis of a rare di-n-acetylated sugar in the lipopolysaccharides of both pseudomonas aeruginosa and bordetella pertussis occurs via an identical scheme despite different gene clusters. | pseudomonas aeruginosa and bordetella pertussis produce lipopolysaccharide (lps) that contains 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid (d-mannac3naca). a five-enzyme biosynthetic pathway that requires wbpa, wbpb, wbpe, wbpd, and wbpi has been proposed for the production of this sugar in p. aeruginosa, based on analysis of genes present in the b-band lps biosynthesis cluster. in the analogous b. pertussis cluster, homologs of wbpb to wbpi were present, but a putative dehydrogenase gene was ... | 2008 | 18621892 |
| disease severity associated with presence in subgingival plaque of porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, and tannerella forsythia, singly or in combination, as detected by nested multiplex pcr. | this study used a nested multiplex pcr method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. the proportions of the patients who harbored these bacteria before periodontal treatment were as follows: tannerella forsythia, 81%; porphyromonas gingivalis, 78%; and aggregatibacter (formerly actinobacillus) actinomycetemcomitans, 47%. at 2 months posttreatment there was ... | 2008 | 18701660 |
| photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. | recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment. | 2008 | 18771363 |
| identification of novel genes in the oral pathogen campylobacter rectus. | a poorly described bacterium, campylobacter rectus, has been implicated as an etiological agent of periodontal disease. the aim of this study was to use a comparative genomics approach to identify genes that contribute to the lifestyle of c. rectus as an oral pathogen. | 2008 | 18793364 |
| periodontal bacterial profiles in pregnant women: response to treatment and associations with birth outcomes in the obstetrics and periodontal therapy (opt) study. | a recent clinical trial (obstetrics and periodontal therapy [opt] study) demonstrated that periodontal therapy during pregnancy improved periodontal outcomes but failed to impact preterm birth. the present study evaluated seven target bacteria, aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), porphyromonas gingivalis, treponema denticola, tannerella forsythia (previously t. forsythensis), prevotella intermedia, campylobacter rectus, and fusobacterium nucle ... | 2008 | 18834241 |
| detection of periodontal pathogens in oral mucous membranes of edentulous individuals. | the purpose of this study was to investigate the colonization of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), prevotella intermedia, and tannerella forsythia (previously t. forsythensis) in the tongue and cheek of newborns and elderly individuals with no teeth. | 2008 | 18834252 |
| granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease. | mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ... | 2009 | 18842116 |
| comparison of culture methods and multiplex pcr for the detection of periodontopathogenic bacteria in biofilm associated with severe forms of periodontitis. | conventional culture methods and multiplex pcr, both of which we have been used for a long time in our clinical microbiology laboratory, were compared for their ability to detect a selected panel of periodontopathic bacteria: aggregatibacter actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, and prevotella intermedia. tests were performed in a single subgingival sample taken from a periodontal diseased site with a probing depth eq ... | 2008 | 18843894 |
| effects of caloric restriction on inflammatory periodontal disease. | dietary caloric restriction (cr) has been found to reduce systemic markers of inflammation and may attenuate the effects of chronic inflammatory conditions. the purpose of this study was to examine the effects of long-term cr on naturally occurring chronic inflammatory periodontal disease in a nonhuman primate model. | 2009 | 18929461 |
| effects of caloric restriction on inflammatory periodontal disease. | dietary caloric restriction (cr) has been found to reduce systemic markers of inflammation and may attenuate the effects of chronic inflammatory conditions. the purpose of this study was to examine the effects of long-term cr on naturally occurring chronic inflammatory periodontal disease in a nonhuman primate model. | 2009 | 18929461 |
| factors affecting human supragingival biofilm composition. ii. tooth position. | little is known regarding the factors that affect the microbial composition of supragingival biofilms. this study was designed to test the hypothesis that tooth location affects the microbial composition of supragingival plaque beyond the effect due to plaque mass as reflected by total dna probe count. | 2009 | 18973539 |
| factors affecting human supragingival biofilm composition. i. plaque mass. | little is known about the factors that affect the microbial composition of supragingival biofilms. this study was designed to examine the relationship between total dna probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. | 2009 | 18973540 |
| demographic determinants for campylobacter infection in england and wales: implications for future epidemiological studies. | despite a significant public health burden the epidemiology of human campylobacter infection remains blurred. the identification of demographic determinants for campylobacter infection is therefore essential for identifying potential areas for intervention. demographic data from an active, population-based sentinel surveillance system for campylobacter infection (from 2000 until 2003, n=15 907) were compared with appropriate denominator data from the 2001 united kingdom census. incidence was hig ... | 2008 | 19000328 |
| optimizing qpcr for the quantification of periodontal pathogens in a complex plaque biofilm. | quantitative pcr (qpcr) has recently been used to quantify microorganisms in complex communities, including dental plaque biofilms. however, there is variability in the qpcr protocols being used. this study was designed to evaluate the validity of two of these variables with the intent of developing a more standardized qpcr protocol. the two variables evaluated were (1) the use of dna content versus actual cell counts to estimate bacterial numbers in mixed plaque samples and (2) the effectivenes ... | 2008 | 19088882 |
| tobacco use increases susceptibility to bacterial infection. | abstract: active smokers and those exposed to secondhand smoke are at increased risk of bacterial infection. tobacco smoke exposure increases susceptibility to respiratory tract infections, including tuberculosis, pneumonia and legionnaires disease; bacterial vaginosis and sexually transmitted diseases, such as chlamydia and gonorrhoea; helicobacter pylori infection; periodontitis; meningitis; otitis media; and post-surgical and nosocomial infections. tobacco smoke compromises the anti-bacterial ... | 2008 | 19094204 |
| increased tlr4 expression in murine placentas after oral infection with periodontal pathogens. | maternal periodontitis has emerged as a putative risk factor for preterm births in humans. the periodontitis-associated dental biofilm is thought to serve as an important source of oral bacteria and related virulence factors that hematogenously disseminate and affect the fetoplacental unit; however the underlying biological mechanisms are yet to be fully elucidated. this study hypothesized that an oral infection with the human periodontal pathogens campylobacter rectus and porphyromonas gingival ... | 2009 | 19101032 |
| oral abscess caused by campylobacter rectus: case report and literature review. | campylobacter rectus was isolated under routine anaerobic conditions (no additional hydrogen gas in the atmosphere) from an oral, nonperiodontal abscess from a patient with gastroesophageal adenocarcinoma. we report the first case of a palate abscess caused by c. rectus and review the literature and atmospheric requirements of this organism. | 2009 | 19109480 |
| periodontal pathogen levels in adolescents before, during, and after fixed orthodontic appliance therapy. | this purpose of this study was to document and investigate changes in periodontal pathogen levels before, during, and after orthodontic treatment in adolescents. | 2009 | 19121507 |
| unreliability of results of pcr detection of helicobacter pylori in clinical or environmental samples. | the aim of this study was to compare published helicobacter pylori primer pairs for their ability to reliably detect h. pylori in gastric biopsy specimens and salivary samples. detection limits of the 26 pcr primer pairs previously described for detection of h. pylori dna in clinical samples were determined. sensitivity and specificity were determined using primers with detection limits of <100 cfu/ml using 50 h. pylori-positive and -negative (by concordance by culture and histology) coded gastr ... | 2009 | 19129407 |
| the vaginal microflora in relation to gingivitis. | gingivitis has been linked to adverse pregnancy outcome (apo). bacterial vaginosis (bv) has been associated with apo. we assessed if bacterial counts in bv is associated with gingivitis suggesting a systemic infectious susceptibilty. | 2009 | 19161595 |
| lysozyme and microbiota in relation to gingivitis and periodontitis. | the aim of this study was to investigate associations between the immune component of the oral cavity, lysozyme, in gingival crevicular fluid and unstimulated saliva, and colonization dynamics of periodontopathogenic bacteria from supra- and subgingival plaque samples in patients with gingivitis or periodontitis. | 2009 | 19179970 |
| bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from japanese subjects. | recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. the purpose of the present study was to investigate the distribution and correlation of 6 oral streptococcal and 6 periodontal species in saliva specimens taken from children and their mothers. | 2009 | 19230860 |