| [reliability and importance of tpha (author's transl)]. | | 1978 | 693458 |
| enteropathogenicity testing of treponema hyodysenteriae in ligated colonic loops of swine. | multiple, ligated loops of swine colon were used as an in vivo model in which to test enteropathogenicity of isolates of treponema hyodysenteriae. gross and microscopic lesions observed in 21 of 22 colonic loops in pigs killed 48 to 72 hours after inoculation with isolates known to be enteropathogenic were characteristic of swine dysentery. these lesions were not observed in 12 loops exposed to uninoculated media or in 12 loops inoculated with nonpathogenic isolates of t hyodysenteriae. the swin ... | 1978 | 697136 |
| microtitration agglutination for detection of treponema hyodysenteriae antibody. | a microtitration agglutination test for the detection of treponema hyodysenteriae antibody in swine and rabbit sera is described. the following methods provided the best test results: antigen produced from the spirochete after a culturing period of 36 to 44 h at 38 degrees c, washed antigen inactivated with 0.01% merthiolate at 4 degrees c for 24 to 36 h, sera heated at 56 degrees c for 30 min, a diluent of phosphate-buffered saline (0.01 m, ph 7.2), and test results read macroscopically after 1 ... | 1978 | 701465 |
| [treponema pallidum passive hemagglutination test with reagents of our production]. | | 1978 | 704941 |
| statistical aspects of the treponemal counts in the tpi test. | a method of counting treponemes in randomly selected visual fields is described. it was found that treponema pallidum is distributed as a poisson distribution among the visual fields and also among samples drawn from the same suspension. immobilized treponemes are binomally distributed among the total number of treponemes. application of the parameters of the poisson and the binomial distributions should be made in order to determine the dosage of t. pallidum in animal experimentation and to eva ... | 1978 | 716919 |
| one species or two? | | 1978 | 728723 |
| experimental infection with treponema hyodysenteriae in guinea pigs. | outbred and inbred (hartley strain) guinea pigs (gp) were inoculated intragastrically with pathogenic and nonpathogenic treponema hyodysenteriae. gp 3 to 16 weeks old received t. hyodysenteriae after a fasting period of 36 to 72 h. infected gp with pathogenic t. hyodysenteriae developed a diarrheal and/or depressive condition, with mucus but not blood in the feces. of 88 gp, 40 had gross lesions resembling those of swine dysentery. lesions were limited mainly to the large intestine. tp used as c ... | 1978 | 730345 |
| uveitis in rabbits with pleural effusion disease. clinical and histopathological observations. | pleural effusion disease (ped) is a generalized infection of laboratory rabbits caused by a virus-like agent. the disease was first described in the late sixties as a mortality problem among rabbits used for the propagation of nichols' pathogenic treponema pallidum in scandinavian laboratories using the t. pallidum immobilization (tpi) test for the serological diagnosis of syphilis. the iridocyclitis, described as a manifestation of ped, has been studied in detail in rabbits experimentally infec ... | 1978 | 735769 |
| cerebrospinal fluid findings in asymptomatic patients with reactive serum fluorescent treponemal antibody absorption tests. | it is common to examine the cerebrospinal fluid in untreated or inadequately treated asymptomatic patients with a reactive serum fluorescent treponemal antibody absorption (fta-abs) test before initiating antibiotic therapy for syphilis. this prospective study evaluated the usefulness of such examination. four hundred thirty-two patients over 40 years old, reporting for annual physical examination, had a serum fta-abs test. thirty-seven (8.6%) patients and 2 of 4 spouses were reactive repeatedly ... | 1978 | 742853 |
| [new routine diagnostic test for syphilis (treponema pallidum-iha test)]. | | 1978 | 746303 |
| isolation and characterization of large treponemes from the bovine rumen. | ten strains of strictly anaerobic spiral organisms were isolated in pure culture from a 10(-7) dilution of bovine rumen contents. three strains were studied in detail. these strains morphologically resembled previously isolated and described rumen treponemes except the new isolates were larger, 0.7 micrometer wide and 12 to 25 micrometer long. they rapidly fermented pectin and, less readily, l-arabinose, inulin, and sucrose. acetic and formic acid were the main fermentation products from pectin; ... | 1979 | 760632 |
| [the lipoid reaction, a new larger concept in medicine]. | | 1975 | 766435 |
| passive transfer of immunity to experimental syphilis in rabbits by immune lymphocytes. | | 1975 | 766726 |
| [model experiments on the effect of igm- and igg-antibodies on the reactivity of treponema pallidum hemagglutination (tpha)-test]. | fractionated sera of patients in different stages of syphilis were examined comparatively with the tpha-fta(igm)- and fta(igg)-test. it was shown that the tpha-test proved to be convenient for early immunologic diagnosis of syphilis. the tpha-test includes not only igg anti-treponemal antibodies but also igm antibodies. in the early stage of syphilis a reactive result of tpha-test can be related exclusively on treponemal specific igm antibodies. | 1976 | 767301 |
| retention of motility of treponema pallidum (nichols virulent strain) in an anaerobic cell culture system and in a cell-free system. | optimum parameters for retention of motility of treponema pallidum (nichols virulent strain) were found by anaerobic co-incubation of the treponeme with rat glial cells and anaerobic incubation in spent medium obtained from glial cells originally grown aerobically. | 1976 | 767359 |
| letter: fta-abs test. | | 1976 | 768532 |
| [electron microscopic study of experimental syphilitic reinduration: on the findings of treponemas in the nerve fibres (author's transl)]. | | 1976 | 768552 |
| [value of tpha in the serological screening of lues]. | in the present work, 21,400 not selected sera were tested with hemagglutination test (tpha) and flocculation test (vdrl) with cardiolipin for screening of syphilis; sera that gave negative results against both tests showed remarkable agreement (97,81%). 468 sera gave positive results to least one of the tests employed in screening and were tested with the following procedures: fluorescent treponemal antibody-absorption (fta/abs) test, complement fixation (by kolmer, modified) with proteic (kp) a ... | 1975 | 769046 |
| demonstration of extracellular material at the surface of pathogenic t. pallidum cells. | pathogenic t. pallidum cells possess and extracellular layer when observed in vivo and in vitro after exposure to ruthenium-red. the extracellular layer is partially removed from a pre-fixed cell by repeated washing in vitro. non-pathogenic treponemes examined in this study do not possess an extracellular layer. it is hoped that the data presented herein will cause our colleagues to take another look at the methods and techniques used for preparing pathogenic t. pallidum cells for practical end- ... | 1976 | 769911 |
| growth and subculture of pathogenic t. pallidum (nichols strain) in bhk-21 cultured tissue cells. | the growth and nine subcultivations of the experimental nichols strain of pathogenic treponema pallidum were successfully accomplished in cultured baby hamster kidney tissue cells (bhk-21) using serum-free media. the number (motile and non-motile cells occurring extracellulary) of cell generations generally increased with each subcultivation, the largest increases (greater than 3-0) occurring in subcultures 4, 5, 7, 8, and 9. also, the number of motile cells decreased with each subcultivation. v ... | 1976 | 769912 |
| letter: specific immobilization of the treponeme--a phenomenon known long ago but sunk into oblivion and rediscovered? | | 1976 | 769914 |
| experimental syphilis vaccines in rabbits. i. differential protection with an adjuvant spectrum. | this study shows that 1 per cent. glutaraldehyde fixation of unwashed treponema pallidum, nichols pathogen, preserves the integrity of the outer envelope as well as the ruthenium-red (rr) staining extracellular slime layer. since other researchers wash their treponemal suspensions before preparing their vaccines, it is likely that the vaccine preparations used in this study contain antigens not tested elsewhere. in an attempt to eliminate the requirement of large numbers of injections to achieve ... | 1976 | 769915 |
| [electron microscopy studies on the masking effect of rolitetracycline (reverin) during experimental syphilis]. | | 1975 | 770217 |
| studies on the treponema pallidum immobilizing activity in normal human serum. 2. serum factors participating in the normal immobilization reaction. | the t. pallidum immobilization reaction to be achieved by unheated normal serum was found to be complement dependent and the results presented suggested that complement was activated via the classical pathway. besides complement, an immobilizing antibody of the igm class was necessary for the immobilization reaction to occur. lysozyme exerted an enhancing effect on the normal serum immobilization reaction. | 1976 | 773102 |
| studies on the treponema pallidum immobilizing activity in normal human serum. 3. the kinetics of immobilization reaction of normal and immune sera. | the influence of immobilizing antibody, complement and lysozyme, on the t. pallidum immobilization reactions of normal and immune sera was studied. lysozyme shortened the lag periods and increased the reaction rates of the reactions of normal and immune sera. at high concentrations of added lysozyme, variations in the concentrations of immobilizing antibody and complement, within a wide range, did not further influence the kinetics of the two reactions. preincubation with lysozyme did not influe ... | 1976 | 773103 |
| letter: stripping technique as an aid in dark-field diagnosis of syphilis. | | 1976 | 773314 |
| virulent treponema pallidum: aerobe or anaerobe. | substrate degradation and protein synthesis served as indicators of metabolism in virulent treponema pallidum. opitmal metabolic activity in these spirochetes was observed at 10 to 20% o2 concentrations, with markedly reduced activity at higher or lower o2 levels or under anaerobiosis; alternate functioning electron acceptors that might substitute for o2 were not found. carbon monoxide and cyanide at concentrations that inactivate cytochrome oxidase were not effective metabolic poisons for t. pa ... | 1976 | 773821 |
| immunity to syphilis: passive transfer in rabbits using serial doses of immune serum. | the influence of prolonged treatment with serial doses of immune serum initiated either 2 or 48 h before intracutaneous challenge with treponema pallidum on the subsequent appearance and development of lesions was studied. whereas treatment with large doses of immune serum initiated 48 h before treponemal challenge caused a delay of 9 to 20 days in the onset of lesions and marked suppression of lesion development. treatment initiated 2 h before treponemal challenge failed to delay the onset of l ... | 1976 | 773833 |
| endotoxemia associated with the jarisch-herxheimer reaction. | | 1976 | 775337 |
| [use of ultrasound-treated treponema antigen for complement fixation test as a sorbent in the immunofluorescence reaction with absorption in syphilis]. | | 1975 | 775842 |
| ultrastructure of cells of treponema pertenue obtained from experimentally infected hamsters. | cells of treponema pertenue gauthier obtained by elution from skin lesions and lymph nodes of experimentally infected hamsters were studied in the electron microscope by means of negative staining. the cells were also examined in thin secions of skin biopsies and lymph nodes. fimbriae were observed on the negatively stained cells. until now fimbriae have not been demonstrated on negatively stained cells of other species of treponema, but at present only one straon of t. pertenue has been studied ... | 1976 | 775884 |
| [use of automatized treponemic (tpha) and cardiolipinic (art) test in the serologic screening of syphilis]. | | 1975 | 776101 |
| cellular and humoral immune response to guinea pig infected with treponema pallidum. | guinea pigs infected intradermally with treponema pallidum nichols strain were examined for the presence of lesions and for cellular and humoral response. of the 26 guinea pigs (77%) demonstrated darkfield positive lesions between 6 and 20 days after infection. animals divided into six groups of 4-5 in each were sacrificed between 2 and 24 weeks. the peritoneal exudate cells, obtained 4 days after injection of oil, were examined by the direct and the cells from lymphoid organs by the indirect ca ... | 1976 | 776839 |
| humoral immunity in experimental syphilis. i. the demonstration of resistance conferred by passive immunization. | evidence of a role for human factor(s) in immunity to experimental syphilis has been provided by the demonstration that passive immunization of rabbits by daily i.v. injections of immune serum significantly delays the appearance and markedly diminishes the severity and duration of lesions which develop after challenge with treponema pallidum. five rabbits were injected daily over 37 days with 3 ml/kg body weight of pooled immune rabbit serum injection with 1.1 x 10(3) t. pallidum, nichols strain ... | 1976 | 778261 |
| humoral immunity in experimental syphilis. ii. the relationship of neutralizing factors in immune serum to acquired resistance. | evidence for a humoral mechanism in immunity to experimental syphilis was provided by the demonstration of immune rabbit serum factor(s) capable of inactivating virulent treponema pallidum, nichols strain, in an in vitro-in vivo neutralization test. after intratesticular infection, rabbits were bled periodically and their resistance to reinfection was determined by challenge with t. pallidum. the results of challenge showed that resistance to reinfection begins to develop by 11 days after infect ... | 1976 | 778262 |
| [survival and humoral response of mice infected with treponema pallidum (author's transl)]. | to judge the pathogenicity of treponema palllidum in mice, we inoculated two groups of animals. 1) one with a normal immune system : adult swiss mice. 2) the other with an immune system presenting a defect in t cells : athymic nude mice. no lesions appeared at the points of inoculation and the survival of these animals was not modified compared with control animals. we have also carried out a study of antitreponema antibodies in adult and new-born swiss mice, using, for the adults, various conc ... | 1976 | 778756 |
| [treponema-pallidum antigen preservation possibilities for the fluorescence serodiagnosis of syphilis]. | | 1975 | 779294 |
| changes in intra-vascular complement and anti-treponemal antibody titres preceding the jarisch-herxheimer reaction in secondary syphilis. | six patients with secondary syphilis and one control subject were observed over a period of up to 8 hr following the administration of either penicillin or erythromycin. serial clinical observations were made and blood samples obtained for complement and serological analysis. the patients showed jarisch-herxheimer reactions (jhr) of varying intensity which were found to be preceded by and to parallel in degree certain changes in complement and syphilitic antibody titres. falls in total haemolyti ... | 1976 | 780021 |
| depression of lymphocyte response to concanavalin a in rabbits infected with treponema pallidum (nichols strain). | peripheral blood lymphocytes isolated from rabbits in the early stages of treponema pallidum infection responded poorly when exposed to concanavalin a in vitro. maximal depression of blastogenesis occurred when lymphocytes were cultured in the presence of autologous serum in comparison with fetal calf or normal homologous rabbit serum. | 1976 | 780277 |
| relative efficacy of clindamycin, erythromycin, and penicillin in treatment of treponema pallidum in skin syphilomas of rabbits. | the currently recommended antibiotic for treatment of fetal syphilis in pregnant women who are allergic to penicillin is erythromycin. however, clindamycin crosses the placenta more effectively than erythromycin. therefore, an in vivo rabbit model of intradermal syphilomas was used to determine the effect of clindamycin compared with the effects of erythromycin and penicillin on the growth of virulent treponema pallidum. the average number of motile treponemes in two, paired, mature lesions was ... | 1976 | 781147 |
| [clinical significances of antitreponemal antibodies with special reference to change of tpha titer by treatment (author's transl)]. | | 1976 | 781344 |
| [fluorescence treponema antibody test in the serological diagnosis of syphilis]. | | 1976 | 781506 |
| [our experiences with the fta-abs test in serological diagnosis of syphilis]. | | 1976 | 781507 |
| in vitro susceptibility of treponema hyodysenteriae to carbadox, virginiamycin, and tylosin. | | 1976 | 782006 |
| [use of antigens from cultured, preserved and lyophilized pathogenic treponema pallidum in the immunofluorescence reaction in syphilis]. | | 1975 | 782070 |
| treponema pallidum in lesions of early syphilis. | | 1976 | 782077 |
| positive fta-abs tests in subjects with corticosteroid-induced uveitis. | of 17 subjects who developed severe anterior nongranulomatous uveitis during or immediatelyy after testing with topical corticosteroid preparations, 14 (82%) had a positive fluorescent-treponemal-antibody absorption (fta-abs) test. no association was found between corticosteroid-induced uveitis and any specific hl-a locus. | 1976 | 782252 |
| [significance of incomplete antibodies for the serodiagnosis of lues. 1. causes of the inagglutinability of fresh isolated treponema pallidum]. | | 1976 | 782946 |
| qualitative evaluation of the reagin screen test. | after a preliminary study of 557 sera used as a procedural training exercise, the reagin screen test (rst) for the macroscopic detection of reagin (as an aid to detecting syphilis) was qualitatively compared to the rapid plasma reagin (rpr) (circle) card test and veneral disease research laboratory (vdrl) slide test on 435 random portions of sera using fluorescent treponemal antibody adsorption (fta-abs) results as a comparative base. a comparison of total agreement (positive and negative) with ... | 1976 | 783187 |
| laboratoire d'hygi'ene de la facult'e de m'edecine de lyon universit'e claude-bernard, domaine rockefeller, 8, avenue rockefeller, 69373 lyon cedex 2 france. | the objective of this study was to investigate the false-positive reactions in the kline, fta200 and fta-abs tests in acute disseminated lupus erythematosus, polyarthritis and controls. though the false-positive kline tests are frequent in autoimmune diseases, false-positive fta200 when made on non lyophilised treponema are uncommon. on the other hand, when lyophilised treponema are used as substrate for the immunofluorescence, false-positive fta200 and fta-abs are more common in the patients wi ... | 1976 | 785344 |
| treponemal antigens in congenital and acquired syphilitic nephritis: demonstration by immunofluorescence studies. | two patients, a 4-month-old infant girl with congenital syphilis and a 45-year-old man with secondary syphilis, had the nephrotic syndrome with glomerulonephritis. immunoglobulins and treponemal antigenic material were seen in the glomeruli of both patients by immunofluorescence microscopic studies of renal tissue. electron micrographs showed subepithelial electron dense deposits along the glomerular basement membrane. this confirms earlier suggestions that the renal injury is of an immune-compl ... | 1976 | 786093 |
| reactivity of lymphocytes from patients with syphilis towards t. pallidum antigen in the leucocyte migration and lymphocyte transformation tests. | the reactivity of lymphocytes to treponema pallidum antigen was studied before and after treatment in nine patients with early syphilis using a leucocyte migration test and a lymphocyte transformation test. lymphocyte reactivity was also investigated in six patients treated for syphilis within the last 4 years, and in five untreated patients with a positive result to the t. pallidum immobilization test, but negative results to other serum tests for syphilis antibodies and without any known expos ... | 1976 | 786437 |
| treponema pallidum infection in subcutaneous polyethylene chambers in rabbits. | male new zealand white rabbits with subcutaneous polyethylene chambers in place for at least 3 months were inoculated by one of the following three methods: (i) "intra-chamber" (ic) inoculation with "normal" chamber fluid: (ii) intratesticular inoculation with treponema pallidum; or (iii) ic inoculation with t. pallidum. rabbits given dexamethasone only, oxisuran only, both drugs, or no drug were observed serially after inoculation. t. pallidum survived and temporarily multiplied to significant ... | 1976 | 786879 |
| the immunology of syphilis. | | 1976 | 786912 |
| correlated transmission electron microscopy (tem) and scanning electron microscopy (sem) of single cells. | | 1976 | 787821 |
| serologic diagnosis of syphilis: an update. | tests used in the serologic diagnosis of syphilis can be divided into two main groups: nontreponemal (reagin) tests and treponemal tests. the most widely used nontreponemal test is the venereal disease research laboratory (vdrl) slide test, and the standard treponemal test is the manual fluorescent treponemal antibody-absorption (fta-abs) test. these tests and other newer tests available for the serologic diagnosis of syphilis are reviewed, with emphasis placed on their current clinical usefulne ... | 1976 | 788161 |
| [the treponema pallidum hemagglutination test as a diagnostic reaction]. | | 1976 | 788217 |
| [treponema pallidum hemagglutination test a new method for the demonstration of syphilis infection]. | | 1976 | 788382 |
| immunoglobulin specificity for the fluorescent treponemal antibody-absorption test conjugate. | quantitative indirect immunofluorescence tests have demonstrated the predominance of immunoglobulin g antibody in most stages of syphilis. a class-specific anti-human immunoglobulin g conjugate is recommended to replace the present fluorescent treponemal antibody-absorption test conjugate. this would allow further definition and standardization of the fluorescent treponemal antibody-absorption test. | 1976 | 789394 |
| examination of various cell culture techniques for co-incubation of virulent treponema pallidum (nichols i strain) under anaerobic conditions. | treponema pallidum (nichols virulent) was incubated with and without cells in cell culture medium reduced to -275 mv ecal, ph 7.3, under deoxygenated conditions. five to ten percent of the treponemes attached to cells and remained motile for at least 120 h in cell-treponeme systems of co-incubation. virulent treponemes could be detected after 120 to 144 h in the supernatant fluids of cell-treponeme co-incubation cultures and in cell-free tubes containing medium harvested from aerobically cultiva ... | 1976 | 789395 |
| persistence of treponema pallidum following penicillin g therapy. report of two cases. | the isolation of treponema pallidum from cerebrospinal fluid (csf) obtained from two adult patients, both of whom had normal csf values except for positive fluorescent treponemal antibody-absorption (fta-abs) tests and both of whom had received currently recommended courses of penicillin, raises important considerations in the treatment of neurosyphilis in adults. | 1976 | 789927 |
| [modern syphilis serology]. | | 1976 | 790796 |
| [judgement and clinical significance of treponema pallidum hemagglutination (tpha) test in syphilis diagnostics]. | | 1976 | 790797 |
| [therapy of syphilis]. | | 1976 | 790798 |
| survival of t. pallidum under microaerobic conditions in cell and tissue cultures. | the survival of pathogenic t. pallidum was studied under microaerobic conditions (partial pressure of oxygen 7 mm hg) with and without tissue cell cultures. as between one third and half of the germs were motile even after 96 hours of incubation, treponemes do not seem to be strictly anerobic as has been generally believed so far. flocks containing enormous numbers of treponemes were observed in the nutrient medium. for their formation, human serum, testicular components from rabbits and modifie ... | 1976 | 790857 |
| immunity in syphilis. studies in active immunity. | support for the concept of the development of immunity during the course of syphilis is avaiable in the literature. in experimental syphilis in rabbits, some immunity is present approximately 3 weeks after infection with treponema pallidum. resistance to re-infection increases to a maximum at approximately 3 months after infection. termination of this state by penicillin treatment within this 3-month period may enable re-infection to be accomplished. attempts to reproduce this state of immunity ... | 1976 | 791454 |
| the enigma of the pathogenesis of the jarisch-herxheimer reaction. | | 1976 | 791455 |
| [the effect of antigen-antibody relations on the sensitivity of the tpi-test]. | | 1976 | 793208 |
| [fluorescence-immunological syphilis diagnosis (fta and fta-abs test) (proceedings)]. | | 1976 | 793223 |
| [hemagglutination procedure in syphilis serology (tpha test) (proceedings)]. | | 1976 | 793224 |
| [value of the tpha test in the diagnosis of late syphilis]. | | 1976 | 794934 |
| [recent data on the biological behavior of treponema pallidum]. | | 1976 | 794984 |
| treponema pallidum in early syphilitic lesions in humans during high-dosage penicillin therapy. an electron microscopical study. | the alterations of early syphilitic infection occuring in the course of high dosage penicillin (120 mega iu, 36 h) as clinical experimental trial has been studied both from the clinical and the electron microscopical views. by electron microscopical studies, findings revealing the localization and the status of treponemes before and during penicillin treatment could be established. before treatment started, the majority of treponemes was of intercellular localization. in the course of treatment ... | 1976 | 795383 |
| problems with beaded fluorescence pattern in fta-abs test. | the significance of false-positive fta-abs fluorescence in connective tissue diseases and other clinical conditions was evaluated by studying the serum from several groups of patients. in 12% of 67 patients without syphilis, serum with an antinuclear antibody (ana) titer of 1:32 or greater gave low intensity fta-abs test fluorescence. in 20% of 150, patients (2.7% with a history of syphilis), serum with rheumatoid factor (rf) titers of 1:640 or greater demonstrated some reactivity. only 1.3% of ... | 1976 | 795797 |
| the microhemagglutination assay for treponema pallidum antibodies (mha-tp), a new treponemal test for syphilis: where does it fit? | the status of a new treponemal test, the microhemagglutination assay for treponema pallidum antibodies (mha-tp) is reviewed and compared with regard to its sensitivity, specificity, and current clinical application to the vdrl slide test, the most widely used non-treponemal test, and to the manual fta-abs test, the standard treponemal test. at present, the mha-tp test seems to combine the simplicity and lower cost of nontreponemal tests and the sensitivity and specificity of treponemal tests, an ... | 1976 | 795798 |
| biopharmacology of syphilotherapy. | data collected from experimental rabbit syphilis and from in vitro studies with nonpathogenic treponemes can be extended only with great caution to human syphilis. the following tentative conclusions may be drawn: penicillin acts on treponema pallidum by interfering with cell wall synthesis. concentrations of penicillin greater than 0.1 mug/ml do not have increased treponemicidal effect. regimens producing penicillinemia for at least 8 days are considerably more efficient than treatment yielding ... | 1976 | 795799 |
| [role of specific serological reactions in the diagnosis of latent and late forms of syphilis]. | | 1976 | 797166 |
| [method of using ultrasonicated treponema antigen]. | | 1976 | 799434 |
| [obtaining of dry and immersion dark field in an ordinary microscope]. | | 1976 | 799441 |
| [activity of procaine penicillin on p and n strains of nichols treponemas from in vivo experiments in rabbits. a comparative study]. | | 1976 | 801667 |
| [specificity of the fta-abs tests in pregnancy]. | | 1976 | 801669 |
| [critical assessment of results obtained in the treatment of early syphilis according to current therapeutic methods and follow-up studies]. | | 1976 | 801670 |
| comparison of the microhemagglutination assay for antibodies to treponema pallidum and the automated fluorescent treponemal antibody-absorption test. | the qualitative microhemagglutination assay for detection of antibodies to treponema pallidum and the automated fluorescent treponemal antibody-absorption tests were directly compared as confirmatory tests for syphilis. they were compared on 2,790 specimens from patients of new york city social hygiene clinics. overall agreement between the two assay methods was 89.6%. only 1.5% of the total specimens tested were reactive by the automated fluorescent treponemal antibody-absorption test, and nonr ... | 1975 | 802487 |
| late syphilis. otologic symptoms and results of the fta-abs test. | the clinician occasionally encounters a patient whose cochleovestibular symptoms are thought to be due to late syphilis mainly on the basis of a reactive fta-abs test. i describe 21 such patients, 11 of whom had either a spurious- or a false-positive reaction. the following suggestions may avoid an inaccurate diagnosis of late syphilis in such patients: (1) repeat all reactive serological tests for syphilis (sts); (2) rule out false-positive reactions; (3) request the degree of fluorescence (1+ ... | 1976 | 803177 |
| isolation and antigenic characteristics of axial filaments from the reiter treponeme. | axial filaments were isolated and purified from reiter treponemes after detergent solubilization of the cells" outer envelope. the axial filaments were separated from the spirochetal cells by shearing, purified by density gradient centrifugation, and fragmented by ultrasonication. acrylamide gel electrophoresis of dissociated filaments revealed two major protein bands. gel diffusion precipitin tests and immunoelectrophoresis between a purified axial filament suspension and anti-reiter treponeme ... | 1975 | 803472 |
| direct antiglobulin tests in narcotic addicted patients. | direct antiglobulin tests (dat) as well as a number of immunological parameters (immunoglobulin levels, latex fixation, vdrl) were studied in 168 narcotic-addicted patients. of these, 94 were untreated narcotic addicts (group i), 61 were methadone maintained patients (group ii), and 13 had completed successful detoxification with methadone and were drug-free (group iii). weakly positive dat were detected in 2.1 per cent of individuals in group i, 18 per cent in group ii, and 15 per cent in group ... | 1975 | 804188 |
| the gynecologist's approach to sexually transmitted infections. | | 1975 | 804366 |
| treponema pallidum (nichols strain) in tissue cultures: cellular attachment, entry, and survival. | the interaction of treponema pallidum (nichols strain) with cultured cells was investigated under aerobic conditions. cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. large numbers of organisms rapidly attached to cultured cells. within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. in addition, t. pallidum attained intracellularity as early as 30 min after inoculation of the cell mo ... | 1975 | 804444 |
| induction of acquired cellular resistance following transfer of thymus-dependent lymphocytes from syphilitic rabbits. | syphilitic rabbits have previously been shown to resist challenge with listeria monocytogenes. thirty days after rabbits were infected with treponema pallidum, transfer of 4 to 6 times 10-8 viable spleen cells along with t. pallidum conferred resistance to listeria on normal recipients. treatment of the spleen cells with anti-thymus serum and complement inhibited or abolished their ability to transfer resistance to listeria. these results support the hypothesis that the ability of syphilitic rab ... | 1975 | 804508 |
| new evidence for the non-infectivity of treponema pallidum for mice. | we have recently shown that syphilitic rabbits are resistant to challenge with listeria monocytogenes. this resistance was thought to reflect stimulation of cell-mediated immunity by active infection with treponema pallidum. we now report data which show that the growth of listeria was not suppressed in mice inoculated with t. pallidum. re-inoculation with t, pallidum or with a large dose of an avirulent treponeme also failed to suppress the growth of listeria. these results contrast with those ... | 1975 | 804955 |
| venereal disease in adolescents. | | 1975 | 806905 |
| personal prophylaxis for venereal diseases. | | 1975 | 807753 |
| [modern serological diagnosis of syphilis (author's transl)]. | after discussion of the antigen structure of treponema pallidum it is shown that only reactions with the pallidum antigen can establish a serological diagnosis of syphilis. reactions with reiter antigen are superfluous, reactions with lipoid antigen (cardiolipin) are non-specific and not very sensitive and consequently only conditionally useful as a test reaction. the behavior of the treponema and lipoid antigen reactions is different in the various stages of lues, and consequently permits certa ... | 1975 | 807840 |
| effect macrophage activation on infection with treponema pallidum. | infection of rabbits with treponema pallidum induces nonspecific acquired cellular resistance (acr) to listeria monocytogenes. this resistance can be adoptively transferred using thymus-dependent lymphocytes. since infections that induce acr are usually brought under control by cellular mechanisms, we sought to determine whether induction of acr in rabbits stimulates resistance to challenge with t. pallidum. when bcg-infected rabbits which suppressed the growth of listeria were challenged intrav ... | 1975 | 809355 |
| [blood-csf kinetics of penicillin g in neurosyphilis (author's transl)]. | measurements of the penicillin concentration in serum and cerebrospinal fluid patients with neurosyphilis showed that conventional therapy can attain effective treponemocidal levels for only a short time or not at all in the csf and brain-tissue, the regions where the treponemas are found in this particular stage of disease. intravenous infusions with high doses (0,5 mega unit penicillin-g/kg body weight in 24 hours) over more than one generation phase of the treponemas (35-43 hours) considerabl ... | 1975 | 809679 |
| sexually transmitted diseases of the anus and rectum. | | 1975 | 810279 |
| [tpha test. comparison with classical lues reactions and the fta-abs test (author's transl)]. | 1. 227 sera from a mixed group of patients were investigated by the tpha test, fta-abs test, the complement fixation reaction with cardiolipin and pallida antigens, the meinicke test ii and the vdrl test, and compared. 2. 124 sera with a diagnosis of "latent seropositive syphilis" were compared in the same way. the tpha, fta-abs and vdrl tests were in very good agreement with a high specificity and sensitivity, so that all 3 methods are suitable for routine diagnosis. 3. a change in the diagnost ... | 1976 | 814423 |
| pulmonary pneumocystosis in nonhuman primates. | pulmonary infection with pneumocystis carinii was detected in two aged owl monkeys (aotus trivirgatus) and two young chimpanzees (pan troglodytes). the clinical histories of the owl monkeys were similar and included progressive weight loss, anorexia, failure to thrive, and death. one of the owl monkeys had no concurrent disease, whereas the other had been experimentally inoculated with treponema pallidum 44 months before death. in both chimpanzees, an underlying myeloproliferative malignant neop ... | 1976 | 814879 |
| studies on the treponema pallidum immobilizing activity in normal human serum. 4. the importance for the outcome of the conventional tpi-test. | t. pallidum immobilizing activity was demonstrated in the majority (84%) of samples of unheated sera obtained from healthy adult subjects. a similar activity was found in unheated sera obtained from some animal species such as monkey and white rat, but not in sera from rabbit or guinea pig. the results of serological studies of acquired, early syphilis in man suggested that the determination of the activity of the naturally occurring igm immobilisins was not influenced by coexisting tpi antibodi ... | 1976 | 817564 |
| the differentiation of an adult's bloodstain from that of a child using an indirect fluorescent antibody technique. | a procedure is described for the detection of specific antimicrobial antibodies in bloodstains. the technique is described and the relationship between antibody concentration and the age of the doner is reported. it is shown that, provided foetal blood can be excluded, an unknown bloodstain may be identified as that of a person over 15 years with a high degree of confidence. | 1975 | 817999 |