| firm but slippery attachment of deinococcus geothermalis. | bacterial biofilms impair the operation of many industrial processes. deinococcus geothermalis is efficient primary biofilm former in paper machine water, functioning as an adhesion platform for secondary biofilm bacteria. it produces thick biofilms on various abiotic surfaces, but the mechanism of attachment is not known. high-resolution field-emission scanning electron microscopy and atomic force microscopy (afm) showed peritrichous adhesion threads mediating the attachment of d. geothermalis ... | 2002 | 11948162 |
| solution structure of a monoheme ferrocytochrome c from shewanella putrefaciens and structural analysis of sequence-similar proteins: functional implications. | within the frame of the characterization of the structure and function of cytochromes c, an 81-amino acid cytochrome c was identified in the genome of shewanella putrefaciens. because of the scarce information about bacterial cytochromes of this type and the large variability in sequences and possibly function, we decided to proceed to its structural characterization. this protein was expressed in escherichia coli and purified. the oxidized species is largely high spin, with a detached methionin ... | 2002 | 11955059 |
| presence of a group ii intron in a multiresistant serratia marcescens strain that harbors three integrons and a novel gene fusion. | we analyzed the role of integrons in the dissemination of antibiotic resistance in a recent multiresistant clinical isolate, serratia marcescens sch88050909 (sch909). this isolate harbors three integrons, all on a 60-kb conjugative plasmid. by pcr, hybridization, and sequencing analyses, we found that integron 1 has the dfra1 and ant(3")-ia cassettes. the first cassette in integron 2 contains the ant(2")-ia gene, separated from its attc site (59-base element) by a 1,971-bp insert containing a gr ... | 2002 | 11959575 |
| genetic complementation in apicomplexan parasites. | a robust forward genetic model for apicomplexa could greatly enhance functional analysis of genes in these important protozoan pathogens. we have developed and successfully tested a genetic complementation strategy based on genomic insertion in toxoplasma gondii. adapting recombination cloning to genomic dna, we show that complementing sequences can be shuttled between parasite genome and bacterial plasmid, providing an efficient tool for the recovery and functional assessment of candidate genes ... | 2002 | 11959921 |
| comparison of adhesion of the food spoilage bacterium shewanella putrefaciens to stainless steel and silver surfaces. | to compare the number of attached shewanella putrefaciens on stainless steel with different silver surfaces, thus evaluating whether silver surfaces could contribute to a higher hygienic status in the food industry. | 2002 | 11972695 |
| sensory, chemical and bacteriological changes during storage of iced squid (todaropsis eblanae). | to relate sensory shelf-life of iced whole and gutted squid to bacterial growth and chemical changes. | 2002 | 11972700 |
| taxis response of various denitrifying bacteria to nitrate and nitrite. | the taxis response of rhodobacter sphaeroides 2.4.1 and 2.4.3, rhodopseudomonas palustris, and agrobacterium tumefaciens to nitrate and nitrite was evaluated by observing the macroscopic behavior of cells suspended in soft agar and incubated under various conditions. r. sphaeroides 2.4.3, which is capable of both nitrate and nitrite reduction, showed a taxis response to both nitrate and nitrite. r. sphaeroides 2.4.1, which contains nitrate reductase but not nitrite reductase, did not show a taxi ... | 2002 | 11976082 |
| enrichment of members of the family geobacteraceae associated with stimulation of dissimilatory metal reduction in uranium-contaminated aquifer sediments. | stimulating microbial reduction of soluble u(vi) to insoluble u(iv) shows promise as a strategy for immobilizing uranium in uranium-contaminated subsurface environments. in order to learn more about which microorganisms might be involved in u(vi) reduction in situ, the changes in the microbial community when u(vi) reduction was stimulated with the addition of acetate were monitored in sediments from three different uranium-contaminated sites in the floodplain of the san juan river in shiprock, n ... | 2002 | 11976101 |
| melanin production and use as a soluble electron shuttle for fe(iii) oxide reduction and as a terminal electron acceptor by shewanella algae bry. | dissimilatory metal-reducing bacteria (dmrb) utilize numerous compounds as terminal electron acceptors, including insoluble iron oxides. the mechanism(s) of insoluble-mineral reduction by dmrb is not well understood. here we report that extracellular melanin is produced by shewanella algae bry. the extracted melanin served as the sole terminal electron acceptor. upon reduction the reduced, soluble melanin reduced insoluble hydrous ferric oxide in the absence of bacteria, thus demonstrating that ... | 2002 | 11976119 |
| comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex. | we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ... | 2002 | 11980954 |
| modeling the inhibition of the bacteral reduction of u(vi) by beta-mno2(s). | pyrolusite (beta-mno2(s)) was used to assess the influence of a competitive electron acceptor on the kinetics of reduction of aqueous uranyl carbonate by a dissimilatory metal-reducing bacterium (dmrb), shewanella putrefaciens strain cn32. the enzymatic reduction of u(vi) and beta-mno2(s) and the abiotic redox reaction between beta-mno2(s) and biogenic uraninite (uo2(s)) were independently investigated to allow for interpretation of studies of u(vi) bioreduction in the presence of beta-mno2(s). ... | 2002 | 11999050 |
| chromate/nitrite interactions in shewanella oneidensis mr-1: evidence for multiple hexavalent chromium [cr(vi)] reduction mechanisms dependent on physiological growth conditions. | inhibition of hexavalent chromium [cr(vi)] reduction due to nitrate and nitrite was observed during tests with shewanella oneidensis mr-1 (previously named shewanella putrefaciens mr-1 and henceforth referred to as mr-1). initial cr(vi) reduction rates were measured at various nitrite concentrations, and a mixed inhibition kinetic model was used to determine the kinetic parameters-maximum cr(vi) reduction rate and inhibition constant [v(max,cr(vi)) and k(i,cr(vi))]. values of v(max,cr(vi)) and k ... | 2002 | 12001169 |
| enhancement of biological reduction of hematite by electron shuttling and fe(ii) complexation. | natural organic matter (nom) enhancement of the biological reduction of hematite (alpha-fe2o3) by the dissimilatory iron-reducing bacterium shewanella putrefaciens strain cn32 was investigated under nongrowth conditions designed to minimize precipitation of biogenic fe(ii). hydrogen served as the electron donor. anthraquinone-2,6-disulfonate (aqds), methyl viologen, and methylene blue [quinones with an ew0 (ph 7) of 0.011 v or less], ferrozine [a strong fe(ii) complexing agent], and characterize ... | 2002 | 12026974 |
| probabilistic clustering of sequences: inferring new bacterial regulons by comparative genomics. | genome-wide comparisons between enteric bacteria yield large sets of conserved putative regulatory sites on a gene-by-gene basis that need to be clustered into regulons. using the assumption that regulatory sites can be represented as samples from weight matrices (wms), we derive a unique probability distribution for assignments of sites into clusters. our algorithm, "procse" (probabilistic clustering of sequences), uses monte carlo sampling of this distribution to partition and align thousands ... | 2002 | 12032281 |
| genetic complementation of an outer membrane cytochrome omcb mutant of shewanella putrefaciens mr-1 requires omcb plus downstream dna. | anaerobically grown cells of the metal-reducing bacterium shewanella putrefaciens mr-1 contain multiple outer membrane (om) cytochromes. a gene replacement mutant (strain omcb1) lacking the om cytochrome omcb is markedly deficient in the reduction of mno2 and exhibits reduced rates of fe(iii) reduction. the levels of other om cytochromes are also decreased in omcb1. complementation of omcb1 with wild-type omcb did not restore any of these defects. however, a 21-kb genomic fragment from mr-1, whi ... | 2002 | 12039733 |
| structure of the phenol-soluble polysaccharide from shewanella putrefaciens strain a6. | the structure of the phenol-soluble polysaccharide from shewanella putrefaciens strain a6 has been elucidated. chemical modifications of the polymer in conjunction with 1h and 13c nmr spectroscopy, including 2d techniques, were employed in the analysis. it is concluded that the repeating unit is composed of two nine-carbon sugars as follows: -->4)-alpha-nonpa-(2-->3)-beta-sugp-(1--> where alpha-nonpa is 5-acetamido-7-acetamidino-8-o-acetyl-3,5,7,9-tetradeoxy-l-glycero-alpha-d-galacto-non-2-uloso ... | 2002 | 12062527 |
| impact of electrode composition on electricity generation in a single-compartment fuel cell using shewanella putrefaciens. | the production of electricity by shewanella putrefaciens in the absence of exogenous electron acceptors was examined in a single compartment fuel cell with different types of electrodes and varying physiological conditions. electricity production was dependent on anode composition, electron donor type and cell concentration. a maximum current of 2.5 ma and a current density of 10.2 mw/m(2)electrode was obtained with a mn(4+) graphite anode, 200 mm sodium lactate and a cell concentration of 3.9 g ... | 2002 | 12073132 |
| diverse bacteria are pathogens of caenorhabditis elegans. | practically and ethically attractive as model systems, invertebrate organisms are increasingly recognized as relevant for the study of bacterial pathogenesis. we show here that the nematode caenorhabditis elegans is susceptible to a surprisingly broad range of bacteria and may constitute a useful model for the study of both pathogens and symbionts. | 2002 | 12117988 |
| regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation. | the riboflavin biosynthesis in bacteria was analyzed using comparative analysis of genes, operons and regulatory elements. a model for regulation based on formation of alternative rna structures involving the rfn elements is suggested. in gram-positive bacteria including actinomycetes, thermotoga, thermus and deinococcus, the riboflavin metabolism and transport genes are predicted to be regulated by transcriptional attenuation, whereas in most gram-negative bacteria, the riboflavin biosynthesis ... | 2002 | 12136096 |
| cluster ii che genes from pseudomonas aeruginosa are required for an optimal chemotactic response. | pseudomonas aeruginosa, a gamma-proteobacterium, is motile by means of a single polar flagellum and is chemotactic to a variety of organic compounds and phosphate. p. aeruginosa has multiple homologues of escherichia coli chemotaxis genes that are organized into five gene clusters. previously, it was demonstrated that genes in cluster i and cluster v are essential for chemotaxis. a third cluster (cluster ii) contains a complete set of che genes, as well as two genes, mcpa and mcpb, encoding meth ... | 2002 | 12142407 |
| microarray transcription profiling of a shewanella oneidensis etra mutant. | dna microarrays were used to examine the effect of an insertional mutation in the shewanella oneidensis etra (electron transport regulator) locus on gene expression under anaerobic conditions. the mrna levels of 69 genes with documented functions in energy and carbon metabolism, regulation, transport, and other cellular processes displayed significant alterations in transcript abundance in an etra-mutant genetic background. this is the first microarray study indicating a possible involvement of ... | 2002 | 12142431 |
| enhancement of hematite bioreduction by natural organic matter. | the effects of natural organic matter (nom), ferrozine, and aqds (anthraquinone-2,6-disulfonate) on the reduction of hematite (alpha-fe2o3) by shewanella putrefaciens cn32 were studied. it has been proposed that nom enhances the reduction of fe(iii) by means of electron shuttling or by fe(ii) complexation. previously both mechanisms were studied separately using "functional analogues" (aqds for electron shuttling and ferrozine for complexation) and are presently compared with seven different nom ... | 2002 | 12144265 |
| the structure of the carbohydrate backbone of the lps from shewanella putrefaciens cn32. | the lipopolysaccharide (lps) from a natural rough strain of shewanella putrefaciens cn32 was analyzed using nmr and mass spectroscopy and chemical methods, and the following structure of its carbohydrate backbone is proposed: beta-galf-(1-->3)-beta-gal-(1-->4)-beta-glc-(1-->4)-alpha-ddhep2petn-(1-->5)-alpha-kdo4p-(1-->6)-beta-glcn4p-(1-->6)-alpha-glcn1p | 2002 | 12151208 |
| r391: a conjugative integrating mosaic comprised of phage, plasmid, and transposon elements. | the conjugative, chromosomally integrating element r391 is the archetype of the incj class of mobile genetic elements. originally found in a south african providencia rettgeri strain, r391 carries antibiotic and mercury resistance traits, as well as genes involved in mutagenic dna repair. while initially described as a plasmid, r391 has subsequently been shown to be integrated into the bacterial chromosome, employing a phage-like integration mechanism closely related to that of the sxt element f ... | 2002 | 12193633 |
| effect of modified atmosphere composition on the metabolism of glucose by brochothrix thermosphacta. | the influence of atmosphere composition on the metabolism of brochothrix thermosphacta was studied by analyzing the consumption of glucose and the production of ethanol, acetic and lactic acids, acetaldehyde, and diacetyl-acetoin under atmospheres containing different combinations of carbon dioxide and oxygen. when glucose was metabolized under oxygen-free atmospheres, lactic acid was one of the main end products, while under atmospheres rich in oxygen mainly acetoin-diacetyl was produced. the p ... | 2002 | 12200298 |
| predicted structure and phyletic distribution of the rna-binding protein hfq. | hfq, a bacterial rna-binding protein, was recently shown to contain the sm1 motif, a characteristic of sm and lsm proteins that function in rna processing events in archaea and eukaryotes. in this report, comparative structural modeling was used to predict a three-dimensional structure of the hfq core sequence. the predicted structure aligns with most major features of the methanobacterium thermoautotrophicum lsm protein structure. conserved residues in hfq are positioned at the same structural ... | 2002 | 12202750 |
| characterization and implications of the cell surface reactivity of calothrix sp. strain kc97. | the cell surface reactivity of the cyanobacterium calothrix sp. strain kc97, an isolate from the krisuvik hot spring, iceland, was investigated in terms of its proton binding behavior and charge characteristics by using acid-base titrations, electrophoretic mobility analysis, and transmission electron microscopy. analysis of titration data with the linear programming optimization method showed that intact filaments were dominated by surface proton binding sites inferred to be carboxyl groups (ac ... | 2002 | 12324327 |
| conservation of the biotin regulon and the bira regulatory signal in eubacteria and archaea. | biotin is a necessary cofactor of numerous biotin-dependent carboxylases in a variety of microorganisms. the strict control of biotin biosynthesis in escherichia coli is mediated by the bifunctional bira protein, which acts both as a biotin-protein ligase and as a transcriptional repressor of the biotin operon. little is known about regulation of biotin biosynthesis in other bacteria. using comparative genomics and phylogenetic analysis, we describe the biotin biosynthetic pathway and the bira r ... | 2002 | 12368242 |
| reduction kinetics of fe(iii), co(iii), u(vi), cr(vi), and tc(vii) in cultures of dissimilatory metal-reducing bacteria. | the reduction kinetics of fe(iii)citrate, fe(iii)nta, co(iii)edta-, u(vi)o(2) (2+), cr(vi)o(4) (2-), and tc(vii)o(4) (-) were studied in cultures of dissimilatory metal reducing bacteria (dmrb): shewanella alga strain bry, shewanella putrefaciens strain cn32, shewanella oneidensis strain mr-1, and geobacter metallireducens strain gs-15. reduction rates were metal specific with the following rate trend: fe(iii)citrate > or = fe(iii)nta > co(iii)edta- >> uo(2)(2+) > cro(4)(2-) > tco(4)(-), except ... | 2002 | 12378605 |
| mtrb is required for proper incorporation of the cytochromes omca and omcb into the outer membrane of shewanella putrefaciens mr-1. | when grown under anaerobic conditions, shewanella putrefaciens mr-1 synthesizes multiple outer membrane (om) cytochromes, some of which have a role in the use of insoluble electron acceptors (e.g., mno2) for anaerobic respiration. the cytochromes omca and omcb are localized to the om and the om-like intermediate-density membrane (im) in mr-1. the components necessary for proper localization of these cytochromes to the om have not been identified. a gene replacement mutant (strain mtrb1) lacking ... | 2002 | 12406753 |
| interactive inhibition of meat spoilage and pathogenic bacteria by lysozyme, nisin and edta in the presence of nitrite and sodium chloride at 24 degrees c. | to develop a nisin- and lysozyme-based antimicrobial treatment for use with processed ham and bologna, in vitro experiments were conducted to determine whether inhibition enhancing interactions occur between the antimicrobials lysozyme, chrisin (a commercial nisin preparation), edta, nacl and nano(2). inhibitory interactions were observed between a number of agents when used against specific pathogenic and spoilage bacteria. the observed interactions included lysozyme with edta (enterococcus fae ... | 2003 | 12423927 |
| structural investigation of the o-specific polysaccharides of morganella morganii consisting of two higher sugars. | the lipopolysaccharide of the bacterium morganella morganii (strain kf 1676, rk 4222) yielded two polysaccharides, ps1 and ps2, when subjected to mild acid degradation followed by gpc. the polysaccharides were studied by 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, 1h,(13)c hmqc, and hmbc experiments. each polysaccharide was found to contain a disaccharide repeating unit consisting of two higher sugars, 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-l-glycero-d-galact ... | 2002 | 12423973 |
| gene islands integrated into trna(gly) genes confer genome diversity on a pseudomonas aeruginosa clone. | intraclonal genome diversity of pseudomonas aeruginosa was studied in one of the most diverse mosaic regions of the p. aeruginosa chromosome. the ca. 110-kb large hypervariable region located near the liph gene in two members of the predominant p. aeruginosa clone c, strain c and strain sg17m, was sequenced. in both strains the region consists of an individual strain-specific gene island of 111 (strain c) or 106 (sg17m) open reading frames (orfs) and of a 7-kb stretch of clone c-specific sequenc ... | 2002 | 12426355 |
| solution structure and characterization of the heme chaperone ccme. | the covalent attachment of the heme cofactor in c-type cytochromes is a surprisingly complex process, which in bacteria involves a number of different proteins. among the latter, the ccme gene product is known to perform a key role in the heme delivery pathway in gram-negative bacteria. the solution structure of the soluble domain of apo-ccme from shewanella putrefaciens was determined through nmr spectroscopy on a 13c,15n-labeled sample. the structure is characterized by a compact core with lar ... | 2002 | 12427019 |
| pseudomonas fluorescens encodes the crohn's disease-associated i2 sequence and t-cell superantigen. | commensal bacteria have emerged as an important disease factor in human crohn's disease (cd) and murine inflammatory bowel disease (ibd) models. we recently isolated i2, a novel gene segment of microbial origin that is associated with human cd and that encodes a t-cell superantigen. to identify the i2 microorganism, blast analysis was used to identify a microbial homologue, pa2885, a novel open reading frame (orf) in the pseudomonas aeruginosa genome. pcr and southern analysis identified pseudom ... | 2002 | 12438326 |
| biochemistry and comparative genomics of sxxk superfamily acyltransferases offer a clue to the mycobacterial paradox: presence of penicillin-susceptible target proteins versus lack of efficiency of penicillin as therapeutic agent. | the bacterial acyltransferases of the sxxk superfamily vary enormously in sequence and function, with conservation of particular amino acid groups and all-alpha and alpha/beta folds. they occur as independent entities (free-standing polypeptides) and as modules linked to other polypeptides (protein fusions). they can be classified into three groups. the group i sxxk d,d-acyltransferases are ubiquitous in the bacterial world. they invariably bear the motifs sxxk, sxn(d), and kt(s)g. anchored in t ... | 2002 | 12456788 |
| shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxic-anoxic interface of the gotland deep in the central baltic sea. | three strains of denitrifying estuarine bacteria, os217(t), 05220 and os226, were characterized for their physiological and biochemical features, fatty acid profiles and their phylogenetic position based on 16s rdna sequences. the strains were isolated from the oxic-anoxic interface of an anoxic basin of the central baltic sea. phylogenetic analyses of the 16s rdna sequences revealed a clear affiliation with members of the genus shewanella of the gamma-proteobacteria. the closest sequence simila ... | 2002 | 12508890 |
| salmonella enterica serovar typhimurium rdoa is growth phase regulated and involved in relaying cpx-induced signals. | the disulfide oxidoreductase, dsba, mediates disulfide bond formation in proteins as they enter or pass through the periplasm of gram-negative bacteria. although dsba function has been well characterized, less is known about the factors that control its expression. previous studies with escherichia coli demonstrated that dsba is part of a two-gene operon that includes an uncharacterized, upstream gene, yihe, that is positively regulated via the cpx stress response pathway. to clarify the role of ... | 2003 | 12511488 |
| differential growth response of colony-forming alpha- and gamma-proteobacteria in dilution culture and nutrient addition experiments from lake kinneret (israel), the eastern mediterranean sea, and the gulf of eilat. | even though it is widely accepted that bacterioplankton growth in lakes and marine ecosystems is determined by the trophic status of the systems, knowledge of the relationship between nutrient concentrations and growth of particular bacterial species is almost nonexistent. to address this question, we performed a series of culture experiments with water from lake kinneret (israel), the eastern mediterranean sea, and the gulf of eilat (northern red sea). in the initial water samples, the proporti ... | 2003 | 12513996 |
| evaluation of microscan autoscan for identification of pseudomonas aeruginosa isolates from cystic fibrosis patients. | accurate identification of gram-negative bacilli from cystic fibrosis (cf) patients is essential. only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as pseudomonas aeruginosa with microscan autoscan. most common misidentifications were pseudomonas fluorescens-pseudomonas putida (i.e., the strain was either p. fluorescens or p. putida, but the system did not make the distinction and yielded the result p. fluorescens/putida) and alcaligenes ... | 2003 | 12517904 |
| comparison between nacl tolerance response and acclimation to cold temperature in shewanella putrefaciens. | two strains of the spoiling bacterium s. putrefaciens showed an adaptation capacity to hyperosmotic shock when they were pretreated with a sublethal concentration of nacl. the maximal tolerance factor for the cip 69.29 strain was obtained when cells were incubated for 1 h in the presence of 1.5% nacl, whereas for the j13.1 strain, an incubation of 15 min in the presence of 1% nacl seemed to be the optimal conditions to harden the cells against a subsequent lethal salt treatment. during nacl adap ... | 2003 | 12567236 |
| in situ environmental cell-transmission electron microscopy study of microbial reduction of chromium(vi) using electron energy loss spectroscopy. | reduction of cr(vi) by the bacterium, shewanella oneidensis (previously classified shewanella putrefaciens strain mr-1), was studied by absorption spectrophotometry and in situ, environmental cell-transmission electron microscopy (ec-tem) coupled with electron energy loss spectroscopy (eels). bacteria from rinsed cultures were placed directly in the environmental cell of the transmission electron microscope and examined under 100 torr pressure. bright field ec-tem images show two distinct popula ... | 2001 | 12597792 |
| photorhabdus species: bioluminescent bacteria as emerging human pathogens? | we report two australian patients with soft tissue infections due to photorhabdus species. recognized as important insect pathogens, photorhabdus spp. are bioluminescent gram-negative bacilli. bacteria belonging to the genus are emerging as a cause of both localized soft tissue and disseminated infections in humans in the united states and australia. the source of infection in humans remains unknown. | 2003 | 12603999 |
| effects of isso2 insertions in structural and regulatory genes of the trimethylamine oxide reductase of shewanella oneidensis. | we have isolated three shewanella oneidensis mutants specifically impaired in trimethylamine oxide (tmao) respiration. the mutations arose from insertions of an isso2 element into tora, torr, and tors, encoding, respectively, the tmao reductase tora, the response regulator torr, and the sensor tors. although tora is not the sole enzyme reducing tmao in s. oneidensis, growth analysis showed that it is the main respiratory tmao reductase. use of a plasmid-borne tore'-lacz fusion confirmed that the ... | 2003 | 12618472 |
| lateral gene transfer and ancient paralogy of operons containing redundant copies of tryptophan-pathway genes in xylella species and in heterocystous cyanobacteria. | tryptophan-pathway genes that exist within an apparent operon-like organization were evaluated as examples of multi-genic genomic regions that contain phylogenetically incongruous genes and coexist with genes outside the operon that are congruous. a seven-gene cluster in xylella fastidiosa includes genes encoding the two subunits of anthranilate synthase, an aryl-coa synthetase, and trpr. a second gene block, present in the anabaena/nostoc lineage, but not in other cyanobacteria, contains a near ... | 2003 | 12620124 |
| electricity production by geobacter sulfurreducens attached to electrodes. | previous studies have suggested that members of the geobacteraceae can use electrodes as electron acceptors for anaerobic respiration. in order to better understand this electron transfer process for energy production, geobacter sulfurreducens was inoculated into chambers in which a graphite electrode served as the sole electron acceptor and acetate or hydrogen was the electron donor. the electron-accepting electrodes were maintained at oxidizing potentials by connecting them to similar electrod ... | 2003 | 12620842 |
| prevalence of bacteria of division tm7 in human subgingival plaque and their association with disease. | members of the uncultivated bacterial division tm7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the presence of tm7 bacteria. tm7 ribosomal dna (rdna) was found in 96% of the samples, and it accounted for approximately 0.3%, on average, of all bacterial rdna in the samples as determine ... | 2003 | 12620860 |
| a metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase. | a zn(cys)(4) center has been found in the c-terminal region of the crystal structure of the anaerobic class iii ribonucleotide reductase (rnr) from bacteriophage t4. the metal center is structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. mutant enzymes of the homologous rnr from escherichia coli, in which the coordinating cysteines, conserved in almost all known class iii rnr sequences, have been mutated into alanines, are shown to be inactive as the result of their ... | 2003 | 12655046 |
| phylogenetic analysis of bacterial populations in an anaerobic microbial consortium capable of degrading saturation concentrations of tetrachloroethylene. | an anaerobic microbial consortium able to biodegrade saturation levels of perchloroethylene (pce) in a column containing a source zone of pce was examined phylogenetically to determine microbial community structure and spatial variation in relation to the pce source. the consortium was comprised of at least 34 members with 7 organisms sharing affiliations with known respiratory or cometabolic dechlorinators. seven other organisms had their closest phylogenetic relative detected in other environm ... | 2003 | 12674344 |
| evaluation of preservative systems in a sunscreen formula by linear regression method. | a sunscreen formula with eight different preservative systems was evaluated by linear regression, pharmacopeial, and the ctfa (cosmetic, toiletry and fragrance association) methods. the preparations were tested against staphylococcus aureus, burkholderia cepacia, shewanella putrefaciens, escherichia coli, and bacillus sp. the linear regression method proved to be useful in the selection of the most effective preservative system used in cosmetic formulation. | 2003 | 12688287 |
| the roles of natural organic matter in chemical and microbial reduction of ferric iron. | although natural organic matter (nom) is known to be redox reactive, the roles and effectiveness of specific functional groups of nom in metal reduction are still a subject of intense investigation. this study entails the investigation of the fe(iii) reduction kinetics and capacity by three fractionated nom subcomponents in the presence or absence of the dissimilatory metal reducing bacterium shewanella putrefaciens cn32. results indicate that nom was able to reduce fe(iii) abiotically; the redu ... | 2003 | 12711432 |
| evaluation of the vitek 2 id-gnb assay for identification of members of the family enterobacteriaceae and other nonenteric gram-negative bacilli and comparison with the vitek gni+ card. | we evaluated the vitek 2 id-gnb identification card (biomérieux, inc., durham, n.c.) for its ability to identify members of the family enterobacteriaceae and other gram-negative bacilli that are isolated in clinical microbiology laboratories. using 482 enteric stock cultures and 103 strains of oxidase-positive, gram-negative glucose-fermenting and nonfermenting bacilli that were maintained at -70 degrees c and passaged three times before use, we inoculated cards according to the manufacturer's d ... | 2003 | 12734254 |
| soft tissue infection and bacteremia caused by shewanella putrefaciens. | shewanella putrefaciens is as yet rarely responsible for clinical syndromes in humans. however, a case involving multiple organs in an elderly male under treatment with appropriate steroids confirms that attention should be devoted to unusual pathogens. | 2003 | 12734291 |
| inhibition of bacterial u(vi) reduction by calcium. | the rapid kinetics of bacterial u(vi) reduction and low solubility of uraninite (uo2,cr) make this process an attractive option for removing uranium from groundwater. nevertheless, conditions that may promote or inhibit u(vi) reduction are not well-defined. recent descriptions of ca-uo2-co3 complexes indicate that these species may dominate the aqueous speciation of u(vi) in many environments. we monitored the bacterial reduction of u(vi) in bicarbonate-buffered solution in the presence and abse ... | 2003 | 12775057 |
| involvement of cyclic amp (camp) and camp receptor protein in anaerobic respiration of shewanella oneidensis. | shewanella oneidensis is a metal reducer that can use several terminal electron acceptors for anaerobic respiration, including fumarate, nitrate, dimethyl sulfoxide (dmso), trimethylamine n-oxide (tmao), nitrite, and insoluble iron and manganese oxides. two s. oneidensis mutants, sr-558 and sr-559, with tn5 insertions in crp, were isolated and analyzed. both mutants were deficient in fe(iii) and mn(iv) reduction. they were also deficient in anaerobic growth with, and reduction of, nitrate, fumar ... | 2003 | 12775705 |
| chemical and biological interactions during nitrate and goethite reduction by shewanella putrefaciens 200. | although previous research has demonstrated that no(3)(-) inhibits microbial fe(iii) reduction in laboratory cultures and natural sediments, the mechanisms of this inhibition have not been fully studied in an environmentally relevant medium that utilizes solid-phase, iron oxide minerals as a fe(iii) source. to study the dynamics of fe and no(3)(-) biogeochemistry when ferric (hydr)oxides are used as the fe(iii) source, shewanella putrefaciens 200 was incubated under anoxic conditions in a low-io ... | 2003 | 12788758 |
| the c-terminal flexible domain of the heme chaperone ccme is important but not essential for its function. | ccme is a heme chaperone active in the cytochrome c maturation pathway of escherichia coli. it first binds heme covalently to strictly conserved histidine h130 and subsequently delivers it to apo-cytochrome c. the recently solved structure of soluble ccme revealed a compact core consisting of a beta-barrel and a flexible c-terminal domain with a short alpha-helical turn. in order to elucidate the function of this poorly conserved domain, ccme was truncated stepwise from the c terminus. removal o ... | 2003 | 12813076 |
| curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant. | a presumed antimicrobial enzyme system, the curvularia haloperoxidase system, was examined with the aim of evaluating its potential as a sanitizing agent. in the presence of hydrogen peroxide, curvularia haloperoxidase facilitates the oxidation of halides, such as chloride, bromide, and iodide, to antimicrobial compounds. the curvularia haloperoxidase system caused several-log-unit reductions in counts of bacteria (pseudomonas spp., escherichia coli, serratia marcescens, aeromonas salmonicida, s ... | 2003 | 12902249 |
| predictive modelling of growth and enzyme production and activity by a cocktail of pseudomonas spp., shewanella putrefaciens and acinetobacter sp. | the possibility was examined of developing a predictive model that combined microbial growth (increase in cellular number) with extracellular lipolytic and proteolytic enzyme activity of a cocktail of four strains of pseudomonas spp. and one strain each of acinetobacter sp. and shewanella putrefaciens. environmental conditions within the following matrix of conditions were examined: temperature 2-20 degrees c, ph value 4.0-7.5 and water activity (a(w)) 0.95-0.995 and a model was constructed, whi ... | 2003 | 12915038 |
| ochrobactrum anthropi misidentified as shewanella putrefaciens. | | 2003 | 12958302 |
| the utilization of sucrose by pseudomonas putrefaciens. | | 1953 | 13069479 |
| some properties of the hexokinase of pseudomonas putrefaciens. | | 1953 | 13117789 |
| [search for alternative secondary structures of rna, regulating expression of bacterial genes]. | expression of many bacterial genes is regulated by formation of alternative secondary rna structure within the leader mrna sequence. our algorithm designed to search for these structures (basing on analysis of one nucleotide sequence) was applied to analyze operons of amino acid biosynthesis in alpha- and gamma-proteobacteria. the attenuators of these operons are predicted for genomes of some poorly known gamma-proteobacteria including shewanella putrefaciens, attenuators of the tryptophan opero ... | 2003 | 14593920 |
| bacterial synergism or antagonism in a gel cassette system. | the growth and the metabolic activity of shewanella putrfaciens, brochothrix thermosphacta, and pseudomonas sp., when cultured individually or in all possible combinations in gel cassettes system supplemented with 0.1% glucose at 5 degrees c, were investigated. the overall outcome was that the coexistence of the above-mentioned microorganisms affected not only each growth rate but also their type of metabolic end products compared to the control cultures. these effects were varied and depended o ... | 2003 | 14660367 |
| chromosome-encoded ambler class d beta-lactamase of shewanella oneidensis as a progenitor of carbapenem-hydrolyzing oxacillinase. | a chromosome-encoded beta-lactamase gene from a shewanella oneidensis reference strain was cloned and expressed in escherichia coli. it encoded a carbapenem-hydrolyzing ambler class d beta-lactamase, oxa-54, that shared 92% amino acid identity with the plasmid-encoded carbapenem-hydrolyzing oxacillinase oxa-48 from klebsiella pneumoniae. this work suggests that shewanella spp. may produce the progenitor of oxacillinases compromising the efficacy of imipenem in clinically relevant gram-negative p ... | 2004 | 14693565 |
| periplasmic cytochrome c3 of desulfovibrio vulgaris is directly involved in h2-mediated metal but not sulfate reduction. | kinetic parameters and the role of cytochrome c(3) in sulfate, fe(iii), and u(vi) reduction were investigated in desulfovibrio vulgaris hildenborough. while sulfate reduction followed michaelis-menten kinetics (k(m) = 220 micro m), loss of fe(iii) and u(vi) was first-order at all concentrations tested. initial reduction rates of all electron acceptors were similar for cells grown with h(2) and sulfate, while cultures grown using lactate and sulfate had similar rates of metal loss but lower sulfa ... | 2004 | 14711670 |
| putrescine, cadaverine, and indole production by bacteria isolated from wild and aquacultured penaeid shrimp stored at 0, 12, 24, and 36 degrees c. | putrescine, cadaverine, and indole production capabilities of bacteria isolated from wild domestic and aquacultured ni-caraguan penaeid shrimp in progressive decomposition states were evaluated. the numbers and types of microorganisms responsible for the production of putrescine, cadaverine, and indole in wild and aquacultured shrimp increased with increasing decomposition temperature and time. throughout the storage experiments, mean aerobic plate counts (log/g) ranged from 4.5 to 9.7 and 4.5 t ... | 2004 | 14717362 |
| proteomic analysis of the sarcosine-insoluble outer membrane fraction of helicobacter pylori strain 26695. | helicobacter pylori causes gastroduodenal disease, which is mediated in part by its outer membrane proteins (omps). to identify omps of h. pylori strain 26695, we performed a proteomic analysis. a sarcosine-insoluble outer membrane fraction was resolved by two-dimensional electrophoresis with immobilized ph gradient strips. most of the protein spots, with molecular masses of 10 to 100 kda, were visible on the gel in the alkaline pi regions (6.0 to 10.0). the proteome of the omps was analyzed by ... | 2004 | 14761989 |
| fructose utilization by pseudomonas putrefaciens. | | 1951 | 14832193 |
| isolation and characterization of integron-containing bacteria without antibiotic selection. | the emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. the use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. the class 1 and 2 integrase genes (inti1 and inti2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (bpw) followed by integrase-specific pcr. integrase-positive bacterial co ... | 2004 | 14982773 |
| [investigation of the bacteria in the seawater of xisha in the south china sea and their antibiotic sensitivity profile]. | to investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of xisha in the south china sea. | 2004 | 15041561 |
| unraveling the function of the rhodospirillum rubrum activator of polyhydroxybutyrate (phb) degradation: the activator is a phb-granule-bound protein (phasin). | efficient hydrolysis of native poly(3-hydroxybutyrate) (nphb) granules in vitro by soluble phb depolymerase of rhodospirillum rubrum requires pretreatment of nphb with an activator compound present in r. rubrum cells (j. m. merrick and m. doudoroff, j. bacteriol. 88:60-71, 1964). edman sequencing of the purified activator (17.4 kda; matrix-assisted laser desorption ionization-time of flight mass spectrometry) revealed identity to a hypothetical protein deduced from a partially sequenced r. rubru ... | 2004 | 15060050 |
| characterisation of a tetrasaccharide released on mild acid hydrolysis of lps from two rough strains of shewanella species representing different dna homology groups. | a reducing tetrasaccharide of the following structure was released by mild acid hydrolysis of r-type lps from shewanella putrefaciens strains ncimb 10472 and 10473. the same tetrasaccharide containing acetal-linked open-chain galnac is present in the core region of lps from s. oneidensis strain mr-1 and may be characteristic of genomic groups ii and iii of s. putrefaciens and related strains. (1s)-d-galanac-(1-->4,6)-alpha-d-galp-(1-->6)-alpha-d-galp-(1-->3)-d-gal. | 2004 | 15063209 |
| real-time quantitative pcr for assessment of abundance of pseudoalteromonas species in marine samples. | a real-time quantitative pcr (rtq-pcr) method for measuring the abundance of pseudoalteromonas species in marine samples is presented. pcr primers targeting a pseudoalteromonas-specific region of the 16s rrna gene were tested at three different levels using database searches (in silico), a selection of pure cultures (in vitro), and a combined denaturing gradient gel electrophoresis and cloning approach on environmental dna (in situ). the rtq-pcr method allowed for the detection of sybr green flu ... | 2004 | 15066834 |
| a comparative genome analysis identifies distinct sorting pathways in gram-positive bacteria. | surface proteins in gram-positive bacteria are frequently required for virulence, and many are attached to the cell wall by sortase enzymes. bacteria frequently encode more than one sortase enzyme and an even larger number of potential sortase substrates that possess an lpxtg-type cell wall sorting signal. in order to elucidate the sorting pathways present in gram-positive bacteria, we performed a comparative analysis of 72 sequenced microbial genomes. we show that sortase enzymes can be partiti ... | 2004 | 15102780 |
| genetic and biochemical characterization of a chromosome-encoded carbapenem-hydrolyzing ambler class d beta-lactamase from shewanella algae. | a chromosome-encoded beta-lactamase gene from shewanella algae clinical isolate kb-1 was cloned and expressed in escherichia coli. it encoded the ambler class d enzyme oxa-55, sharing less than 55% identity with any other oxacillinases. although conferring a narrow-spectrum beta-lactam resistance phenotype, oxa-55 had carbapenem-hydrolyzing activity that mirrored the reduced susceptibility to imipenem observed in s. algae kb-1. very similar oxacillinases were found in other s. algae isolates. | 2004 | 15105119 |
| prolinks: a database of protein functional linkages derived from coevolution. | the advent of whole-genome sequencing has led to methods that infer protein function and linkages. we have combined four such algorithms (phylogenetic profile, rosetta stone, gene neighbor and gene cluster) in a single database--prolinks--that spans 83 organisms and includes 10 million high-confidence links. the proteome navigator tool allows users to browse predicted linkage networks interactively, providing accompanying annotation from public databases. the prolinks database and the proteome n ... | 2004 | 15128449 |
| interaction of the sliding clamp beta-subunit and hda, a dnaa-related protein. | in escherichia coli, interactions between the replication initiation protein dnaa, the beta subunit of dna polymerase iii (the sliding clamp protein), and hda, the recently identified dnaa-related protein, are required to convert the active atp-bound form of dnaa to an inactive adp-bound form through the accelerated hydrolysis of atp. this rapid hydrolysis of atp is proposed to be the main mechanism that blocks multiple initiations during cell cycle and acts as a molecular switch from initiation ... | 2004 | 15150238 |
| new method for rapid and sensitive quantification of sulphide-producing bacteria in fish from arctic and temperate waters. | the offensive, fishy, rotten h2s-off-odours in spoiled, aerobically and cold stored fish from arctic and temperate waters are generally caused by sulphide-producing bacteria (spb), mainly shewanella putrefaciens. in the present work, a new, rapid, simple and accurate method for estimation of the spb content in fish from these areas is described. the quantification is based on the formation rate of iron sulphide during growth of spbs incubated at 30 degrees c in a liquid growth medium containing ... | 2004 | 15163589 |
| a core catalytic domain of the tyra protein family: arogenate dehydrogenase from synechocystis. | the tyra protein family includes prephenate dehydrogenases, cyclohexadienyl dehydrogenases and tyra(a)s (arogenate dehydrogenases). tyra(a) from synechocystis sp. pcc 6803, encoding a 30 kda tyra(a) protein, was cloned into an overexpression vector in escherichia coli. tyra(a) was then purified to apparent homogeneity and characterized. this protein is a model structure for a catalytic core domain in the tyra superfamily, uncomplicated by allosteric or fused domains. competitive inhibitors actin ... | 2004 | 15171683 |
| identification of mdod, an mdog paralog which encodes a twin-arginine-dependent periplasmic protein that controls osmoregulated periplasmic glucan backbone structures. | osmoregulated periplasmic glucans (opgs) of escherichia coli are anionic and highly branched oligosaccharides that accumulate in the periplasmic space in response to low osmolarity of the medium. the glucan length, ranging from 5 to 12 glucose residues, is under strict control. two genes that form an operon, mdogh, govern glucose backbone synthesis. the new gene mdod, which appears to be a paralog of mdog, was characterized in this study. cassette inactivation of mdod resulted in production of o ... | 2004 | 15175282 |
| explorative multivariate analyses of 16s rrna gene data from microbial communities in modified-atmosphere-packed salmon and coalfish. | modified-atmosphere packaging (map) of foods in combination with low-temperature storage extends product shelf life by limiting microbial growth. we investigated the microbial biodiversity of map salmon and coalfish by using an explorative approach and analyzing both the total amounts of bacteria and the microbial group composition (both aerobic and anaerobic bacteria). real-time pcr analyses revealed a surprisingly large difference in the microbial loads for the different fish samples. the micr ... | 2004 | 15294842 |
| shewanella oneidensis mr-1 restores menaquinone synthesis to a menaquinone-negative mutant. | the mechanisms underlying the use of insoluble electron acceptors by metal-reducing bacteria, such as shewanella oneidensis mr-1, are currently under intensive study. current models for shuttling electrons across the outer membrane (om) of mr-1 include roles for om cytochromes and the possible excretion of a redox shuttle. while mr-1 is able to release a substance that restores the ability of a menaquinone (mk)-negative mutant, cma-1, to reduce the humic acid analog anthraquinone-2,6-disulfonate ... | 2004 | 15345428 |
| alkaline anaerobic respiration: isolation and characterization of a novel alkaliphilic and metal-reducing bacterium. | iron-reducing enrichments were obtained from leachate ponds at the u.s. borax company in boron, calif. based on partial small-subunit (ssu) rrna gene sequences (approximately 500 nucleotides), six isolates shared 98.9% nucleotide identity. as a representative, the isolate qymf was selected for further analysis. qymf could be grown with fe(iii)-citrate, fe(iii)-edta, co(iii)-edta, or cr(vi) as electron acceptors, and yeast extract and lactate could serve as electron donors. growth during iron red ... | 2004 | 15345448 |
| a rare cause of wound infection: shewanella putrefaciens. | shewanella putrefaciens has a wide geographical distribution, including all forms of sea water, fresh water, fish and soil. in humans, it is an unusual pathogen of wound infections. in this report, a wound infection was presented in a previously healthy man. | 2004 | 15370659 |
| the mutation of pseudomonas putrefaciens to glucose utilization and its enzymatic basis. | | 1950 | 15436448 |
| potential role of a novel psychrotolerant member of the family geobacteraceae, geopsychrobacter electrodiphilus gen. nov., sp. nov., in electricity production by a marine sediment fuel cell. | previous studies have shown that members of the family geobacteraceae that attach to the anodes of sediment fuel cells are directly involved in harvesting electricity by oxidizing organic compounds to carbon dioxide and transferring the electrons to the anode. in order to learn more about this process, microorganisms from the anode surface of a marine sediment fuel cell were enriched and isolated with fe(iii) oxide. two unique marine isolates were recovered, strains a1(t) and a2. they are gram-n ... | 2004 | 15466546 |
| global transcriptome analysis of the heat shock response of shewanella oneidensis. | shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities. however, the genetic basis and regulatory mechanisms underlying the ability of s. oneidensis to survive and adapt to various environmentally relevant stresses is poorly understood. to define this organism's molecular response to elevated growth temperatures, temporal gene expression profiles were examined in cells subjected to heat stress by using whole-genome dna micr ... | 2004 | 15516594 |
| shewanella profunda sp. nov., isolated from deep marine sediment of the nankai trough. | a novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain lt13a(t)) was isolated from a deep sediment layer in the nankai trough (leg 190, ocean drilling program) off the coast of japan. this organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. max ... | 2004 | 15545415 |
| preliminary evaluation of the api 20ne and rapid nf plus systems for rapid identification of burkholderia pseudomallei and b. mallei. | we evaluated the api 20ne and the rapid nf plus systems with 58 burkholderia pseudomallei and 23 b. mallei strains for identification of these agents, but neither was reliable for confirmatory identification, with only 0 to 60% strains identified accurately. a greater diversity of strains in the system databases would be beneficial. | 2005 | 15635021 |
| actinide and metal toxicity to prospective bioremediation bacteria. | bacteria may be beneficial for alleviating actinide contaminant migration through processes such as bioaccumulation or metal reduction. however, sites with radioactive contamination often contain multiple additional contaminants, including metals and organic chelators. bacteria-based bioremediation requires that the microorganism functions in the presence of the target contaminant, as well as other contaminants. here, we evaluate the toxicity of actinides, metals and chelators to two different b ... | 2005 | 15643939 |
| manual and automated instrumentation for identification of enterobacteriaceae and other aerobic gram-negative bacilli. | identification of gram-negative bacilli, both enteric and nonenteric, by conventional methods is not realistic for clinical microbiology laboratories performing routine cultures in today's world. the use of commercial kits, either manual or automated, to identify these organisms is a common practice. the advent of rapid or "spot" testing has eliminated the need for some commonly isolated organisms to be identified with the systems approach. commercially available systems provide more in-depth id ... | 2005 | 15653824 |
| shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. | a highly efficient dye-decolorizing bacterium, strain s12t, was isolated from activated sludge of a textile-printing waste-water treatment plant in guangzhou, china. the cells were gram-negative and motile by means of a single polar flagellum. the strain was capable of anaerobic growth either by fermentation of glucose or by anaerobic respiration and utilized a variety of electron acceptors, including nitrate, iron oxide and thiosulfate. the physiological properties, tested by using the biolog g ... | 2005 | 15653901 |
| anaerobic, nitrate-dependent oxidation of u(iv) oxide minerals by the chemolithoautotrophic bacterium thiobacillus denitrificans. | under anaerobic conditions and at circumneutral ph, cells of the widely distributed, obligate chemolithoautotrophic bacterium thiobacillus denitrificans oxidatively dissolved synthetic and biogenic u(iv) oxides (uraninite) in nitrate-dependent fashion: u(iv) oxidation required the presence of nitrate and was strongly correlated with nitrate consumption. this is the first report of anaerobic u(iv) oxidation by an autotrophic bacterium. | 2005 | 15812053 |
| monochloramine versus sodium hypochlorite as antimicrobial agents for reducing populations of bacteria on broiler chicken carcasses. | studies were conducted to compare the effect of sodium hypochlorite (sh) versus monochloramine (mon) on bacterial populations associated with broiler chicken carcasses. in study 1, nominal populations (6.5 to 7.5 log cfu) of escherichia coli, listeria monocytogenes, pseudomonas fluorescens, salmonella serovars, shewanella putrefaciens, and staphylococcus aureus were exposed to sterilized chiller water (controls) or sterilized chiller water containing 50 ppm sh or mon. sh at 50 ppm eliminated all ... | 2005 | 15830667 |
| microbiological quality changes in the intestine of hybrid tilapia (oreochromis niloticus x oreochromis aureus) in fresh and frozen storage condition. | the goal of this study was to monitor the quantitative and qualitative bacterial flora in the intestine of hybrid tilapia in fresh fish and fish kept in frozen storage conditions for 1 year. | 2005 | 15892747 |
| surface structure and nanomechanical properties of shewanella putrefaciens bacteria at two ph values (4 and 10) determined by atomic force microscopy. | the nanomechanical properties of gram-negative bacteria (shewanella putrefaciens) were investigated in situ in aqueous solutions at two ph values, specifically, 4 and 10, by atomic force microscopy (afm). for both ph values, the approach force curves exhibited subsequent nonlinear and linear regimens that were related to the progressive indentation of the afm tip in the bacterial cell wall, including a priori polymeric fringe (nonlinear part), while the linear part was ascribed to compression of ... | 2005 | 15901713 |
| [opportunistic infections caused by shewanella, new emergent bacteria]. | shewanella putrefaciens and shewanella algae are gram negative, nonfermentative and oxidative bacilli whose the main phenotypic feature is the production of hydrogen sulfide gas. widespread in the environment, both s. putrefaciens and s. algae species are rare human bacteria although they are reported with increasing frequency as a cause of opportunistic infection in humans, such as skin and soft tissue infections and bacteremia. chronic infections of the lower limbs and liver disease have been ... | 2005 | 15914286 |
| microbial diversity in ultra-high-pressure rocks and fluids from the chinese continental scientific drilling project in china. | microbial communities in ultra-high-pressure (uhp) rocks and drilling fluids from the chinese continental scientific drilling project were characterized. the rocks had a porosity of 1 to 3.5% and a permeability of approximately 0.5 mdarcy. abundant fluid and gas inclusions were present in the minerals. the rocks contained significant amounts of fe2o3, feo, p2o5, and nitrate (3 to 16 ppm). acridine orange direct counting and phospholipid fatty acid analysis indicated that the total counts in the ... | 2005 | 15933024 |
| ['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp]. | gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ... | 2005 | 15991478 |