| alterations in pulmonary surfactant composition and activity after experimental lung transplantation. | pulmonary surfactant facilitates breathing by reducing the surface tension at the air/liquid interface. we examined the effect of experimental lung transplantation on the phospholipid pool sizes of alveolar surfactant large and small aggregates, the composition of the large aggregates, the surface tension-reducing ability of lipid extract surfactant, and the leakage of serum proteins into the lung. a double-lung block from the donor animal was stored for 2 or 12 h after perfusion with either eur ... | 1993 | 8317801 |
| [p-31 mr spectroscopy of excised pig liver]. | this study used p-31 mr spectroscopy (p-31 mrs) to evaluate the viability of a pig liver preserved with perfusate. pig liver was excised and preserved in cooled euro-collins solution after canulation of the portal vein and perfusion with the solution. p-31 mrs was performed on a 1.5 tesla machine. p-31 mrs showed that the beta-peak of atp in the preserved pig liver diminished within two hours. p-31 mrs of pig liver with perfusion of cooled and oxygenated euro-collins solution revealed a signific ... | 1993 | 8327320 |
| the effect of the addition of albumin to cardioplegic and preservation solutions on the isolated rat heart. | this experimental study evaluates the effect of the addition of albumin to cardioplegic and preservation solutions on isolated rat hearts. male wistar rat hearts were arrested with a 4 degrees c crystalloid cardioplegic solution. all hearts were immersed for 8 hours in a 4 degrees c euro-collins solution. they were divided into four groups: group i, the cardioplegic solution was used for cardiac arrest, and the 4 degrees c euro-collins solution did not contain albumin; group ii, the cardioplegic ... | 1993 | 8329420 |
| human islet purification: a prospective comparison of euro-ficoll and bovine serum albumin density gradients. | euro-ficoll (ef) and bovine serum albumin (bsa) are the two most commonly used media for the density gradient purification of human pancreatic islets. the aim of this study was to compare these two media with respect to the efficiency of human islet isolation. ten human pancreata were collagenase-digested, and samples of digest were separated on either a continuous linear density gradient of bsa or a discontinuous gradient of ef (1.108/1.096/1.037/euro-collins). efficiency of islet purification ... | 1993 | 8329732 |
| the efficiency of pulmonary blood transport following single lung transplantation. | perioperative right ventricular (rv) dysfunction remains a significant problem following single lung transplantation (slt), especially in patients with pulmonary hypertension. total rv power (wt), a determinant of rv function, is the sum of the mean component (wm) which contributes to actual blood flow and the oscillatory component (wo) which is the energy expended on arterial pulsation. calculation of wo is possible only through harmonic analysis of pulmonary arterial (pa) pressure and flow wav ... | 1993 | 8331922 |
| evidence of the existence of a soluble mediator of cold preservation injury. | a study was designed to determine whether soluble mediators of injury are released during cold preservation. a first set of livers consisting of three groups was stored in cold euro-collins solution. these were a control group stored for 10 min (group 1), an experimental group stored for 16 hr (group 2), and an "antiprotease" group to which a cocktail of antiproteases had been added, which was also stored for 16 hr (group 3). the preservation solution in these livers was washed out at the end of ... | 1993 | 8333066 |
| beneficial effect of the addition of nitroglycerin to the cardioplegic solution on the cold-stored reperfused isolated rat heart. | we discuss here the effect of the addition of trinitroglycerin to the cardioplegic solution on the isolated rat heart after eight hours of storage. this effect was evaluated by measuring cardiac function as well as myocardial calcium and enzymes. male wistar rats were divided into three groups based on the concentration of trinitroglycerine in the cardioplegic solution. in the control group, the fluid used was a crystalloid cardioplegic solution (k+ 25 meq/l) without trinitroglycerin and for gro ... | 1993 | 8341002 |
| normothermic liver perfusion ex situ: a resuscitation tool for hepatic grafts damaged by warm ischemia. | ex situ perfusion of the liver has been proposed as a preservation tool for organs to be grafted; in this study, we have investigated whether this procedure could help to reverse damage caused by prolonged ischaemia. the liver was explanted from 6 pigs under general anaesthesia. the organs were subject to warm ischaemia (37 degrees c) for 30 min., cooled in situ by portal infusion of euro collins, excised and maintained ischaemic at 4 degrees c for 30 min. more. afterwards, normothermic perfusio ... | 1993 | 8343355 |
| injury to cultured liver endothelial cells during cold preservation: energy-dependent versus energy-deficiency injury. | previously, we demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in university of wisconsin (uw) solution. here, the effects of histidine-tryptophan-ketoglutarate (htk) and euro-collins (ec) solutions on these cells were studied. in htk solution, 83% +/- 4% of the cells had lost viability after 9 h of incubation at 4 degrees c. the addition of cyanide (1 mm) to simulate hypoxic conditions protected the cells to the extent that only 9% +/- 1% of th ... | 1993 | 8347268 |
| protective effect of the paf antagonist bn 52021 in an experimental renal warm ischemia model. | platelet activating factor is involved in warm ischemic damage. we studied the effect of the paf receptor antagonist bn 52021 in an experimental model of 60 min of renal warm ischemia in which the left kidney was flushed with euro-collins solution and a right nephrectomy was performed. eighty wistar rats were divided into a sham-operated group, two control groups, and four study groups, according to the dosage and route of bn 52021 administration. bn 52021 was used in the flush solution at conce ... | 1993 | 8347271 |
| effects of water restriction on digestive function in two macropodid marsupials from divergent habitats and the feral goat. | the effects of water restriction on digestive function in the euro (macropus robustus erubescens) found in the arid zone of inland australia, the eastern wallaroo (m.r.robustus) from more mesic regions of eastern australia, and the feral goat (capra hircus) found throughout the range of m. robustus, were compared in order to examine some physiological adaptations required by herbivores for the exploitation of arid environments. eight animals of each species were held in individual metabolism cag ... | 1993 | 8349886 |
| effects of trehalose in preservation of canine lung for transplants. | the effect of trehalose, a non-reducing disaccharide which stabilizes and protects membranes, in the preservation of canine lungs was examined when euro-collins solution was basically used as a preservant. in group i, five lungs were perfused and preserved in an euro-collins solution modified by replacing the glucose with 35.0 g/l of trehalose. five control lungs (group ii) were perfused and preserved with euro-collins solution containing 35.0 g/l glucose. in both groups, no vasodilators were us ... | 1993 | 8367858 |
| cold- and cryopreservation of human liver and kidney slices. | tissue slices may provide a rapid and economical way of determining cold ischemic effects on human liver and kidney cell viability and metabolism. in contrast to isolated hepatocyte cultures, tissue slices offer an in vitro system which more closely resembles the in vivo situation because of the differentiation and functional heterogeneity of the slice. in this study, human liver and kidney slices were cold stored for 10 days in belzers university of wisconsin (uw), euro-collins, and modified sa ... | 1993 | 8370311 |
| [the effect of additional albumin with optimal concentration on the reperfusion after preservation of isolated rat hearts]. | we studied the effect of additional albumin in preservation solution on reperfusion after immersion of isolated rat hearts by measuring cardiac function, myocardial enzymes and calcium. male wistar rats weighing 300 gr to 450 gr were used. isolated rat hearts were arrested by cardioplegic solution and washed out with preservation solution. then, the hearts were immersed at 4 degrees c for six hours. the group was divided into four by the concentration of additional albumin in preservation soluti ... | 1993 | 8377753 |
| [effect of na-l-glutamate on mitochondrial function of atp synthesis after prolonged preservation in rat heart]. | we studied the effect of na-l-glutamate (glu) on mitochondrial functions of atp synthesis after prolonged hypothermic preservation of rat hearts. we divided isolated rat hearts into the following three groups: group a, hearts without immersion as control; group b, hearts immersed in euro-collins solution (e-c) without glu for 24 h at 4 degrees c; group c, hearts immersed in e-c with 10mm glu for 24 h at 4 degrees c. number of each group used was 6. mitochondrial functions of atp production were ... | 1993 | 8377763 |
| the effect of superoxide production on the replantation of rat limbs after cold ischemia. | in order to clarify the effect of superoxide anion radicals on the "no-reflow phenomenon," the authors examined the utility of simple cold preservation of rat limbs in replantation. they quantified such radicals, derived from erythrocytes and plasma in the preserved limb, using a chemiluminescence method employing a derivative of luciferin (cla). eighty-four lewis rat limbs were preserved at 4 degrees c in euro-collins solution for 10 to 120 hr, and grafted orthotopically to isogeneic rats by mi ... | 1993 | 8380871 |
| the effects of allopurinol and sod on lipid peroxidation and energy metabolism in the liver after ischemia in an aerobic/anaerobic persufflation. | this study was aimed at examining the vulnerability of the liver to oxygen-free radicals upon reoxygenation after prolonged ischemia. livers from male wistar rats were first flushed with ringer's and euro-collins solutions. after ischemic storage in krebs-henseleit solution at 37 degrees c for 60 min and in euro-collins solution at 4 degrees c for another 60 min, they were then persufflated with either gaseous o2 or n2 for 30 min at 37 degrees c, and rinsed again with ringer's solution. enzyme c ... | 1993 | 8400677 |
| role of the hepatovasculature in free radical mediated reperfusion damage of the liver. | this study was undertaken in order to assess the role of purely circulation-related effects upon free-radical-mediated reperfusion injury in the liver by comparing the respective effects of the oxygen free-radical scavenger superoxide dismutase (sod) and the vasodilative action of papaverine in an ischemia/reperfusion model of the liver. livers from male wistar rats were rinsed blood free via the portal vein and stored ischemically (60 min at 37 degrees c in krebs-henseleit solution and 60 min a ... | 1993 | 8404987 |
| [successful 24-hour pulmonary preservation in the use of hyperbaric oxygen]. | 24-hour lung preservation with modified euro-collins (e-c) solution under hyperbaric oxygen (ohp) was studied in the canine single lung transplantation model. a prostaglandin i2 (pgi2) was administered before cardiac arrest by st. thomas solution. lungs were flushed with modified e-c solution, then heart and lungs were excised en-bloc and immersed in 4 degrees c modified e-c solution for 24 hours in control group (n = 5). in ohp group (n = 5), heart and lung block were preserved similarly in the ... | 1993 | 8409595 |
| the effect of different solutions for organ preservation on immediate postischemic pancreatic function in vitro. | the present study compares the effect of organ preservation with euro-collins solution, cardioplegic histidine-tryptophan-ketoglutarate solution, and university of wisconsin solution on immediate pancreatic function after cold storage at 4 degrees c for 24 hr. postischemic organ quality of a porcine pancreas preparation was tested by quantification of physiological and biomedical parameters in a one-line reperfusion system. during reperfusion with a constant arterial pressure the arteriovenous f ... | 1993 | 8420035 |
| transplantation of canine lung allografts preserved in uw solution for 24 hours. | to evaluate the efficacy of university of wisconsin (uw) solution for lung preservation, we performed canine lung allotransplantation with lungs preserved in uw solution. donor lungs from eight dogs were flushed with uw solution and lungs from four dogs with euro-collins solution, and these were preserved for 24 hr in cold storage at 4-6 degrees c. all recipient dogs were given cyclosporine at a dose of 20 mg/kg/day until death. none of the four dogs that received a lung allograft flushed with e ... | 1993 | 8420040 |
| improved recovery of heart transplants with a specific kit of preservation solutions. | in the course of cardiac transplantation, donor hearts undergo a four-step sequence of events (arrest, cold storage, global ischemia during implantation, and reperfusion) during which myocardial damage can occur. we tested the hypothesis that the functional recovery of these hearts could be improved by exposure to two interdependently formulated preservation solutions throughout this four-step sequence. solution i was used as a perfusion and storage medium during the first three steps, and solut ... | 1993 | 8429664 |
| metabolic activity of freshly prepared and cryopreserved hepatocytes in monolayer culture. | the successful use of isolated hepatocytes for transplantation will, no doubt, require cryopreservation of the cells. however, cryopreservation results in the loss of viability of isolated hepatocytes. in this study a method is described that allows recovery of viable hepatocytes after cryopreservation. freshly prepared and cryopreserved hepatocytes (1.8 m me2so in euro-collins solution) were suspended in dulbecco's culture medium and allowed to form monolayers in primaria t-25 culture flasks. v ... | 1993 | 8440125 |
| long-chain acyl-coenzyme a thioesters and renal hypothermic ischemic injury: effects of glycine flush. | the effects of hypothermic ischemia utilizing euro-collins flush on renal tissue long-chain activated fatty acid content was studied in dogs. also, the ability of the simple amino acid glycine to complex these acyl thioesters was also investigated. renal inner cortex was found to contain (in increasing amounts) myristoyl-, palmitoleoyl-, palmitoyl-, arachidonyl-, and oleoyl-coenzyme a throughout the 3 days of cold ischemia. although the amounts of individual long-chain acyl-coa compounds varied ... | 1993 | 8440127 |
| preservation of small bowel with university of wisconsin and euro-collins solution. | | 1993 | 8442210 |
| an in vitro method for comparing the efficacy of two preservation solutions in one canine liver using the 5'-nucleotidase assay. | the activity and localization of the plasma membrane-bound enzyme 5'-nucleotidase (5'-nt) in liver tissue are sensitive parameters of ischemic damage. the value of 5'-nt as a marker of liver graft viability was studied in relation to liver preservation. in six mongrel dogs, the main right and left branches of the portal vein were cannulated and flushed separately in situ with cold university of wisconsin (uw) solution and euro-collins (ec) solution, respectively. after hepatectomy, the right and ... | 1993 | 8452636 |
| action plan for a tobacco-free europe: implementation in central and eastern europe, 1993-94. who regional office for europe. | major political and social changes in central and eastern european (cee) countries offer new opportunities and challenges in tobacco control. action against tobacco in cee countries is part of an overall european strategy, the action plan for a tobacco-free europe. several cee countries have voiced their concern about the deterioration of tobacco control and about the entry of the international tobacco industry. in response, the regional office has set up a framework for an international task fo ... | 1993 | 8461862 |
| a comparison of intracellular solutions for donor heart preservation. | numerous solutions have been advocated for the preservation of donor hearts, and there has been much interest in universal and intracellular preservation solutions. this study compared the effects of euro-collins, university of wisconsin, and bretschneider's solutions with the use of an in vitro human right atrial muscle preparation to assess recovery of function after a 24-hour period of simulated cardiac arrest. there were no statistically significant differences among groups in length, weight ... | 1993 | 8469000 |
| the value of histological study by surface analysis of preserved and injected pancreatic grafts: a prospective controlled study in dogs. | to evaluate the effects of pancreas preservation and pancreatic duct obliteration on the endocrine pancreas, three groups of dogs were used: a control group (six) in which histologic analysis of normal pancreas was performed and two randomized groups (seven) from which the caudal pancreas was auto-transplanted, injected with fibrin glue and removed on the 28th day. in group a, each graft was flushed out with euro-collins' solution and immediately transplanted. in group b, each graft was preserve ... | 1993 | 8473081 |
| cadaver lung donors: effect of preharvest ventilation on graft function. | the pulmonary donor pool would increase substantially if lungs could be safely transplanted after cessation of circulation. to determine whether ventilation of cadaver lungs could improve graft function, canine donors were sacrificed and then ventilated with 100% oxygen (n = 6) or 100% nitrogen (n = 6); 6 served as nonventilated controls. four hours after death, the lungs were flushed with modified euro-collins solution and harvested. controls were ventilated with 100% oxygen only during flush a ... | 1993 | 8494430 |
| protein synthesis and gene expression in transplanted and postischemic livers. | the expression of some genes has been comparatively studied in transplanted rat liver and in liver reperfused after ischemia in situ. experiments on protein synthesis by tissue slices from cold-stored or transplanted livers show that rat livers that retain a good capacity for protein synthesis during storage undergo a profound impairment in the capacity for protein synthesis during the first hours after transplantation. this recovers in the following hours. there is never any indication of synth ... | 1993 | 8497910 |
| improved ultrastructural lung preservation with prostaglandin e1 as donor pretreatment in a primate model of heart-lung transplantation. | donor pretreatment with prostaglandin e1 as a pulmonary vasodilator has developed as a simple, effective means to provide excellent preservation in heart-lung transplantation. this study was undertaken to investigate the degree of ultrastructural preservation of the lung with prostaglandin e1 and other pulmonary vasodilators in a primate heart-lung transplantation model. heart-lung transplantation was performed in 14 african green monkeys. donor cardiac preservation was achieved with cold crysta ... | 1993 | 8501946 |
| effect of a free radical scavenger on cadaver lung transplantation. | the pulmonary donor pool would increase substantially if lungs could be safely transplanted after cessation of circulation. to determine whether the addition of the free radical scavenger dimethylthiourea to the perfusate of cadaver lungs could improve graft function, canine donors were sacrificed, and lungs retrieved 2 hours after death. in a blinded fashion, dimethylthiourea was added to the modified euro-collins solution and infused into recipients (n = 9) perioperatively; a placebo was inclu ... | 1993 | 8512395 |
| perfadex is superior to euro-collins solution regarding 24-hour preservation of vascular function. | the aim of this study was to compare perfadex with euro-collins solution regarding 24-hour preservation of endothelium-dependent relaxation and vascular smooth muscle function. | 1995 | 8526601 |
| morphometric characterisation of the fine structure of human type ii pneumocytes. | pulmonary type ii pneumocytes have been examined by scanning electron microscopy (sem), transmission electron microscopy (tem), and morphometry in numerous mammals. until now, the fine structure of the human type ii pneumocyte has not been studied by means of morphometry. | 1995 | 8540632 |
| regeneration after in situ flushing of partially hepatectomised rat livers. | the effect of various components of the transplant procedure upon the regenerative process in reduced liver grafts is not known. in this study, partially hepatectomised rat liver remnants were flushed with 5 ml of either ringer's lactate, euro collins solution or university of wisconsin solution at 4 degrees c and then 5 ml ringer's lactate at 4 degrees c. after partial hepatectomy alone, the peak increase in thymidine kinase was measured at 24 hours (36,021 +/- 8,060 disintegrations per minute ... | 1995 | 8545730 |
| delta opioid extends hypothermic preservation time of the lung. | to test the hypothesis that a delta opioid, dadle ([d-ala2, d-leu5]-enkephalin), could protect tissue from ischemic damage during hypothermic lung preservation, we studied three groups of rats. in group 1 (n = 8), lung function was studied immediately after harvesting. in group 2 (n = 8), the lung was flushed with 4 degrees c euro-collins solution and preserved for 24 hours. in group 3 (n = 8), the lung was flushed with 4 degrees c euro-collins solution plus dadle (1 mg/kg) and preserved for 24 ... | 1996 | 8551774 |
| the f(ab')2 fragment of an anti-icam-1 monoclonal antibody attenuates liver injury after orthotopic liver transplantation. | long-term storage of liver grafts results in increased adhesion of leukocytes onto the sinusoidal walls. this eventually leads to posttransplant graft damage through disturbances of hepatic microcirculation. intracellular adhesion molecule-1 (icam-1) is known to be involved in attachment of leukocytes. this study was designed to examine whether icam-1 participated in the pathogenesis of posttransplant liver injury. inbred lewis rats were used as both donors and recipients to avoid immunoreactivi ... | 1996 | 8560583 |
| improved 20-hour canine lung preservation with a new solution--et-kyoto solution. | we developed a new solution, et-kyoto (et-k) solution, and compared its efficacy in 20 h lung preservation with that of euro-collins (ec) solution in a canine left lung transplantation model. the lungs were flushed with et-k solution (et-k group, n = 5), with ec solution plus prostaglandin e1 (pge1) pretreatment (ec + pge1 group, n = 6) or with ec solution (ec group, n = 6), and were stored at 4 degrees c for 20 h. the function of left lung grafts was assessed 40, 70, and 130 min after transplan ... | 1995 | 8562098 |
| [histopathological changes in the small intestine after hypothermic preservation with university of wisconsin and euro-collins solutions. experimental study]. | the objective of this study is to compare small bowel histopathologic alterations under hypothermic preservation with university of wisconsin (uw) and euro-collins (ec) solutions. | 1995 | 8574227 |
| the mechanism of action of the two-layer cold storage method in canine pancreas preservation--protection of pancreatic microvascular endothelium. | we have demonstrated that oxygenation of a pancreas during preservation by a two-layer method leads continued atp production to maintain cellular integrity and produces an extended period of preserved pancreatic viability. the aim of this study is to examine the effect of atp vs. oxygenation per se on viability of nonparenchymal cell (vascular endothelium), using 2.4 dinitrophenol, an uncoupler of mitochondrial oxidative phosphorylation. mongrel dogs of both sexes, weighing 12-18kg were used. un ... | 1995 | 8577086 |
| in vitro evaluation of donor liver preservation fluids on human hepatocyte function. | successful liver transplantation depends on adequate preservation of cellular function. we therefore tested the effects of two currently used liver preservation fluids, euro-collins (ec) solution and university of wisconsin (uw) solution, on the viability and some functional activities of hepatocytes isolated from human livers. cells in primary culture were maintained under hypoxic (95% n2/5% co2) and hypothermic (4 degrees c) conditions for 24 h, either in ec or uw solution. this treatment did ... | 1995 | 8579732 |
| preservation of skin free-flap using trehalose. | in this study, we looked for a solution able to preserve traumatically amputated tissues such as nose tip, ear, and scalp for at least 48 hr that was easy to handle and low in price. trehalose is a nonreducing disaccharide that can protect many prokaryotes, fungi, and yeasts against adverse conditions by stabilizing cell membranes. in order to study its protective effect, 60 skin free-flaps of the ears of rabbits were preserved in euro-collins (ec) solution or in modified euro-collins solution i ... | 1996 | 8606500 |
| pentoxifylline in flush solution improves early lung allograft function. | postischemic ischemia reperfusion injury is a frequent and unpredictable problem in clinical lung transplantation. pentoxifylline (ptx) has a number of effects that could decrease reperfusion injury: reduced neutrophil adhesion to endothelium, decreased production of tumor necrosis factor, decreased platelet aggregation, and increased production of vasodilatory prostaglandins by vascular endothelium. we have demonstrated previously that ptx administered before storage and again during reperfusio ... | 1996 | 8607655 |
| importance of calcium in long-term preservation of the vasculature. | the aim was to investigate the effect of calcium in organ preservation solutions with respect to 36-hour preservation of vascular smooth muscle function and endothelium-dependent relaxation. | 1996 | 8607675 |
| do splanchnic viscera contribute to liver preservation reperfusion injury? | preservation-reperfusion injury of hepatic allografts is thought to be associated with kupffer cell activation and tnf release from the transplanted organ. confirmation that the allograft is the source of this tnf in an in vivo model is difficult because of rapid equilibration of this cytokine into all compartments. a novel experimental design was devised to aid in accurate localization of the site of tnf release following a orthotopic liver transplant (olt). in the first group (anhepatic), live ... | 1996 | 8610407 |
| lidocaine reduces reperfusion injury and neutrophil migration in canine lung allografts. | depletion of neutrophils (pmns) and inhibition of pmn endothelial adhesion ameliorate post-ischemic lung reperfusion injury. lidocaine reduces pmn adhesion to endothelial surfaces in vivo, and inhibits upregulation of pmn-cd11b/cd18 (mac-1) in vitro. we evaluated the effect of lidocaine on reperfusion injury, pmn adhesion, and pmn migration in preserved lung allografts. | 1996 | 8619724 |
| low-potassium solution for lung preservation in the setting of high-flow reperfusion. | we previously demonstrated that standard preservation using euro-collins solution impairs lung function in the setting of high-flow reperfusion because of potassium-induced vasoconstriction. preservation strategies for single-lung transplantation are an important factor in patients with pulmonary hypertension. this study investigates the hypothesis that low-potassium preservation solution will improve function of lungs subjected to high-flow reperfusion. | 1996 | 8619728 |
| long-term cryopreservation can prevent rejection of canine tracheal allografts with preservation of graft viability. | we previously reported that cryopreservation of tracheas for 1 month was possible in a canine tracheal autotransplantation model with use of a preservative solution containing trehalose. realizing that the allogenicity of many organs is decreased by freezing, we examined the possibility of immunosuppressant-free canine tracheal allotransplantation after long-term cryopreservation. six to 10 rings of the trachea were removed from donor dogs (n = 12), immersed in the preservative solution, and cry ... | 1996 | 8622315 |
| enhanced isolated lung function after ischemia with anti-intercellular adhesion molecule antibody. | the binding of leukocytes to intercellular adhesion molecules expressed on endothelial surfaces during ischemia and subsequent reperfusion initiates leukocyte-mediated reperfusion injury. interruption of this leukocyte-endothelium interaction may therefore prevent reperfusion injury. in an isolated, ventilated, blood-perfused rabbit lung preparation, we studied the effect of a monoclonal anti-intercellular adhesion molecule antibody on lung function during reperfusion. lungs were harvested with ... | 1996 | 8622317 |
| cadaver lungs for transplantation. effect of ventilation with alveolar gas. | in an effort to increase the donor pool for lung transplantation (ltx), we have demonstrated the feasibility of ltx from circulation-arrested cadavers in a canine ltx model. we hypothesized that ventilation of the cadaver lung with alveolar gas (20% o2, 5% co2, balance n2) (ag) would be superior to ventilation with 100% oxygen (o2) after circulatory arrest of the donor. twelve mongrel dogs were intubated, heparinized and euthanized by pentothal injection and ventilated with ag (n=6) or o2 (n=6). ... | 1996 | 8623177 |
| effects of university of wisconsin and euro-collins solutions on interstitial pulmonary edema in isolated rat lungs. | macromolecules are present in lung preservation solutions to limit liquid filtration out of the pulmonary circulation and minimize pulmonary edema. we tested the effectiveness of these molecules by measuring interstitial edema in rat lungs perfused with macromolecular solutions (university of wisconsin [uw] solution and euro-collins solution supplemented with modified pentastarch [pentafraction, pen]) or with solutions that lacked macromolecules (uw solution with pen and euro-collins solution.) ... | 1996 | 8623178 |
| glyoxalase ii and glutathione levels in rat liver mitochondria during cold storage in euro-collins and university of wisconsin solutions. | glutathione and glyoxalase ii levels were evaluated in cytosolic and mitochondrial compartments of rat liver after 7 and 24 hr of cold storage in university of wisconsin (uw) and euro-collins solutions. 1-4 a similar time-dependent depletion of cytosolic glutathione up to about 60% of control values was observed in both euro-collins and uw solutions. cytosolic glyoxalase ii showed activity oscillations in livers stored in euro-collins but not in uw. mitochondrial glutathione and glyoxalase ii we ... | 1996 | 8629310 |
| critical importance of the first 10 minutes of lung graft reperfusion after hypothermic storage. | we have shown previously that lung graft function can be improved by achieving reperfusion with stepwise increments of perfusion pressure over 60 minutes. this study aimed to establish whether similar benefit could be achieved with a shorter, simpler protocol and different storage conditions. | 1996 | 8651760 |
| an arterial blood gas analysis in the canine bilateral sequential lung transplant model flushed with modified euro-collins solution. | | 1996 | 8658906 |
| ultrastructural changes in canine lung preserved in newly developed solutions. | the present study was undertaken to clarify the effect of differences in the ionic composition of a preservation solution by investigating ultrastructures of pulmonary endothelial cells and oxygenation ability of preserved lungs after reperfusion. the relation between the ultrastructural changes and the oxygenation ability was also examined. canine lungs flushed with an extracellular-type (et)-kyoto solution (group a, n = 6), with an intracellular-type-kyoto solution and prostaglandin el (group ... | 1996 | 8661244 |
| pulmonary allograft ischemic time: an important predictor of survival after lung transplantation. | traditionally organ availability in human lung transplantation has been limited by aiming to keep the graft ischemic time under 6 hours. to maximize organ supply in a country with a widely spread population, we have routinely procured organs beyond this time. our experience outlines the clinical consequences of a prolonged allograft ischemic time. | 1996 | 8672519 |
| euro-collins solution exacerbates lung injury in the setting of high-flow reperfusion. | single-lung transplantation has been abandoned for the treatment of pulmonary hypertension by many centers because of overperfusion of the graft following implantation. euro-collins solution is currently used for lung preservation despite the vasoconstrictive effect of this intracellular-type solution. we hypothesized that high-flow reperfusion, alone or in combination with euro-collins-induced vasoconstriction, may cause lung dysfunction. twenty-eight new zealand white rabbit lungs were harvest ... | 1996 | 8691854 |
| hyperinflation of canine lung allografts during storage increases reperfusion pulmonary edema. | the optimal state of inflation for lung allografts during preservation is not known. we previously showed that hyperinflation of canine lung allografts during storage improved posttransplant graft function as measured during 10 minutes of contralateral pulmonary artery occlusion. however, we have also shown that hyperinflation during storage increases pulmonary capillary permeability. it is possible that short-term total cardiac perfusion through the transplanted left lung (for assessment) may n ... | 1996 | 8691891 |
| a systematic study of hypothermic lung preservation solutions: euro-collins solution. | testing lung function after preservation is difficult because a suitable model is still lacking; thus, the effectiveness of different solutions for lung preservation has not been confirmed. this study tested the effectiveness of euro-collins solution alone for hypothermic preservation of rat lungs. | 1996 | 8694591 |
| assessment of endothelial preservation in human cell cultures. | impairment of microcirculation due to endothelial cell damage must be considered a limiting factor in organ preservation. the present study aims at a quantitative assessment of preservation-induced injury in cultured human endothelial cells. | 1996 | 8694617 |
| evaluation of a new solution containing trehalose for twenty-hour canine lung preservation. | we examined the efficacy of two new preservation solutions containing trehalose--an extracellular type (et-k) of solution and an intracellular type (it-k) of solution--in relation to that of euro-collins (ec) solution in 20-h canine lung preservation. canine lungs were flushed with one of the three solutions (n = 5 for each solution) after pretreatment with pge1 (20 micrograms/kg) and were stored for 20 h at 4 degrees c. the left lungs were transplanted and evaluated to 6 h post transplant. in t ... | 1996 | 8723186 |
| retrograde versus antegrade flush in canine left lung preservation for six hours. | retrograde flush through the left atrium is now used by some investigators in clinical lung preservation. however, to date there are no studies which compare its result with that of routine antegrade flush. | 1996 | 8732599 |
| normothermic renal artery perfusion: a comparison of perfusates. | hypothermia and preservative perfusates have been used to decrease ischemic renal injury. this study was performed to identify the preservative function of perfusates independent of the effects of hypothermia. rats underwent 45 minutes of renal ischemia. rectal and renal parenchyma temperatures were monitored and maintained within 1 degree c of normal. perfusates were university of wisconsin solution (uw), euro-collins solution, normal saline solution, and ringer's lactate solution. a nonperfuse ... | 1996 | 8733863 |
| effect of ischemia and three different perfusion solutions on the rabbit epiphyseal growth plate. | an isolated vascularized knee joint model was used (1) to determine the minimum ischemia time that produced growth retarding damage to the epiphyseal plate and (2) to evaluate whether intra-arterial perfusion could reduce the ischemia damage. experiment 1 consisted of 31 rabbits in 5 groups: 0, 2,4,6, and 8 hr of warm ischemia produced by clamping the pedicle. experiment 2 consisted of 18 rabbits in which 10 ml of one of (1) euro-collins, (2) university of wisconsin solution, or (3) heparinized ... | 1995 | 8747288 |
| inhaled nitric oxide improves lung allograft function after prolonged storage. | morbidity caused by early allograft dysfunction, manifested by a progressive increase in pulmonary vascular resistance and a decrease in oxygenation, remains a serious problem in lung transplantation. inhalation of nitric oxide, an essential homeostatic molecule, has been shown to have beneficial effects on a variety of acute lung injuries. the purpose of the present study was to investigate the effect of inhaled nitric oxide on posttransplant function of canine left lung allografts. fourteen do ... | 1996 | 8751493 |
| insulin-like growth factor-i attenuates delayed graft function in a canine renal autotransplantation model. | insulin-like growth factor-i (igf-i) has been shown to accelerate recovery in animal models of ischemic or toxic acute renal injury. ischemic renal injury is frequently encountered after cadaveric transplantation manifested as delayed graft function. this study was performed to determine whether perfusion of kidneys with preservation solution supplemented with igf-i would improve the course of renal injury in a canine autotransplantation model of delayed graft function. | 1996 | 8751586 |
| a new family of entodiniomorph protozoa from the marsupial forestomach, with descriptions of a new genus and five new species. | a unique group of entodiniomorph protozoa was found in forestomach contents from quokka (setonix brachyurus), western grey kangaroo (macropus fuliginosus), red kangaroo (macropus rufus) and euro (macropus robustus erubescens). a new genus, macropodinium n.g., containing five new species, is described. three species are described from forestomach contents of the quokka: macropodinium baldense n. sp., macropodinium moiri n. sp. and macropodinium setonixum n. sp. a single species, macropodinium enn ... | 1996 | 8768432 |
| prostacyclin analog op2507 prevents pulmonary arterial and airway constriction during lung preservation and reperfusion. | the effects of op2507 on lung preservation with cold euro-collins solution and during the reperfusion period were evaluated. | 1996 | 8771502 |
| lung retrieval from cadaver donors with nonbeating hearts: optimal preservation solution. | we have previously studied the time course of pulmonary cell viability, ultrastructural damage, and adenine nucleotide metabolites after circulatory arrest in a rat model to investigate the feasibility of lung retrieval for transplantation from cadavers. this study was designed to investigate the effect of hypothermic flush and subsequent 4-hour storage with either modified euro-collins or university of wisconsin solution on lungs retrieved 4 hours after death. | 1996 | 8771505 |
| beneficial effect of thromboxane a2 synthetase inhibitor (oky-046) on 24-hr simple hypothermic preservation of the canine pancreas graft. | this study was performed to investigate the potential protective effect of oky-046, an inhibitor of thromboxane a2 synthetase, on the viability of 24-hr preserved canine segmental pancreas graft. each graft was preserved in either cold euro-collins (e-c) solution (control group; n = 5) or e c+oky-046(10(-4)m) solution (oky-046 group; n = 5). in both groups, grafts were preserved for 24 hr and calculated the total levels of 6keto-prostaglandin f1 alpha (6ket-pgf1 alpha) and thromboxane b2(txb2) i ... | 1995 | 8775620 |
| donor lungs from ventilated cadavers: impart of a free radical scavenger. | the shortage of donors for lung transplantation may be alleviated with the use of lungs retrieved from cadavers. the purpose of this study was to determine whether a free radical scavenger, dimethylthiourea, would improve the function of lungs retrieved from ventilated cadavers. | 1996 | 8777211 |
| nitroprusside ameliorates lung allograft reperfusion injury. | nitric oxide is believed to play a critical role in the maintenance of vascular integrity through its interaction with neutrophils, platelets, and cellular components of the vessel wall. it has been reported that endogenous nitric oxide level was depressed after ischemia, reperfusion, or both. furthermore, exogenous as well as endogenous nitric oxide decreases reperfusion-induced vascular dysfunction. we hypothesized that nitroprusside, a potent nitric oxide donor, might enhance lung preservatio ... | 1996 | 8784010 |
| maximal preservation time of tracheal allografts. the paris-sud university lung transplantation group. | our objective was to study the maximal preservation time of directly revascularized tracheal allografts in immunosuppressed piglets. | 1995 | 8787449 |
| prostaglandin e1 or prostacyclin in euro-collins solution fails to improve lung preservation. | in search of the ideal composition of the flush solution for pulmonary preservation, we studied the effects of prostaglandin e1 (pge1) and prostacyclin as an additive to euro-collins solution (ecs) on pulmonary hemodynamics and gas exchange in a porcine single lung transplantation model using extracorporeal circulation and right heart bypass. | 1995 | 8787453 |
| beneficial effect of steroid on myocardial preservation in isolated rat hearts. | myocardial injury after heart storage has been known to contribute to an increase in myocardial calcium content or oxygen free radical production. the present experiment was designed to determine the inhibitory effect of steroid in the preservation solution on myocardial injury during simple immersion of isolated rat hearts. the experimental group was divided into 5 sub-groups according to the concentration of steroid added to the solution. group i was stored in preservation solution containing ... | 1995 | 8788373 |
| canine double-lung transplantation with cadaveric donors. | if lungs could be retrieved from cadavers after circulatory arrest, the critical shortage of donors for lung transplantation might be alleviated. to assess gas exchange after transplantation of lungs from cadaveric donors, we performed double-lung transplantation through sequential thoracotomies in 12 dogs. donors were sacrificed by intravenous pentobarbital injection and then ventilated with 100% oxygen. lungs were harvested 2 hours (n = 6) or 4 hours (n = 6) after death and flushed with 2 l mo ... | 1996 | 8800142 |
| neutrophil endopeptidase inhibitor improves pulmonary function during reperfusion after eighteen-hour preservation. | reperfusion injury remains a significant problem after lung transplantation and is thought to be in part mediated by neutrophils. ulinastatin inhibits release of elastase and cathepsin g from neutrophil granules. we hypothesized that inhibition of these neutrophi endopeptidases (proteases) would attenuate pulmonary reperfusion injury. | 1996 | 8800146 |
| expression of intercellular and vascular cell adhesion molecules and class ii major histocompatibility antigens in human lungs: lack of influence by conditions of organ preservation. | the expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and class ii major histocompatibility complex antigens was studied in control lung tissue and preserved human donor lungs. the three controls were represented by wedge biopsy specimens taken from non-neoplastic lung surrounding bronchogenic carcinomas. | 1995 | 8800726 |
| cold- and cryopreservation of dog liver and kidney slices. | the use of tissue slices in culture could decrease the number of animals used in health-related research and decrease experimental variation. this reduction may come about particularly if the methods of cold- and cryopreserving tissue slices are perfected, and one can conduct sequential in vitro experiments into xenobiotic metabolism, organ-specific toxicity, or organ-specific biochemical processes with tissue slices. with this goal in mind, dog liver and kidney slices were placed in cold storag ... | 1996 | 8812095 |
| health beliefs as predictors of hiv-preventive behavior and ethnic differences in prediction. | the incidence of a variety of safer-sex behaviors was predicted, using five measures from the health belief model (janz & becker, 1984): perceived susceptibility, perceived severity, self-efficacy, social support, and perceived barriers. the participants, 424 u.s. undergraduates at six schools, completed a written questionnaire. the results of multiple regression analyses indicated that perceived susceptibility, self-efficacy, and social support predicted many safer-sex behaviors. although the h ... | 1996 | 8851449 |
| hiv infection and aids in jerusalem: a microcosm of illness in israel. | during the decade 1985-94, 158 individuals (124 males, 34 females) with hiv/aids were seen at the aids clinic of hadassah university hospital. they comprised 10% of the total reported number of hiv-infected israelis and included 6% of all reported hiv-infected ethiopians and all hiv-infected west bank palestinians. most individuals (82%) were self-referred; 12% were detected through the local hiv screening clinic. risk groups for aids were homo/bisexuality (39%), heterosexual relations (30%), in ... | 1996 | 8865824 |
| potential of 31p magnetic resonance spectroscopy in monitoring the viability of human renal grafts stored in euro-collins perfusion solution. | the relative concentrations of inorganic phosphate and phosphomonoesters (pme) of 18 human cadaveric kidneys stored in euro-collins perfusion solution were measured by 31p mr spectroscopy. the signals of intracellular inorganic phosphate (pii) and inorganic phosphate contained in the perfusion solution (pie) were separated by the deconvolution technique. the ratio of the signal intensities of phosphomonoesters and intracellular inorganic phosphate (pme/pii) was used as a marker of kidney viabili ... | 1995 | 8869268 |
| amelioration of pulmonary allograft injury by administering a second rinse solution. | the use of rinse solutions before reperfusing liver allografts has been shown to reduce cell death in rats. carolina rinse solution (an extracellular solution that contains antioxidants, vasodilators, and other substrates that help prevent ischemia-reperfusion injury) has also been shown to improve liver function clinically in liver transplant recipients. this pilot study evaluates the value of a second pulmonary artery flush before reperfusion of a lung graft. | 1996 | 8873728 |
| comparison of low potassium euro-collins solution and standard euro-collins solution in an extracorporeal rat heart-lung model. | euro-collins solution (ec) is routinely used in lung transplantation. the high potassium of ec, however, may damage the vascular endothelium, thereby contributing to postischemic reperfusion injury. to assess the influence of the potassium concentration on lung preservation, we evaluated the effect of a "low potassium euro-collins solution" (lpec), in which the sodium and potassium concentrations were reversed. | 1996 | 8875169 |
| rat whole-limb viability after cold immersion using university of wisconsin and euro-collins solutions. | the purpose of this study was to compare university of wisconsin (uw) solution with euro-collins (ec) solution in their cold preservation effects on rat limbs. thirty-six lewis rat limbs were preserved in ec solution (n=18) or uw solution (n=18) at 4 degrees c for 72 hr, and grafted orthotopically to a syngeneic rat using microsurgical techniques. the surgeon was blinded to the solution used. we evaluated the vascular patency rate and death rate of both groups at day 7 after surgery and performe ... | 1996 | 8878378 |
| morphological changes of human pulmonary-artery endothelial cells after 12-hours hypothermic storage in organ-preservation solutions. | pulmonary endothelial cells are known to be fundamental for lung preservation and one of the most serious limiting factors observed during transplantation is the stress to which these cells are subjected. on this premise, strenuous efforts should be made to select and employ the preservation solution best able to prolong ischemia time and thus prevent cytotoxic effects. the aim of this study was to identify the solution with minimum toxicity on endothelial cells. for this purpose, we analysed th ... | 1996 | 8885690 |
| influence of dextrans on lung preservation: is the molecular weight important? | the addition of dextran with a molecular weight of 40,000 dalton in pulmonary preservation solutions has proved to be beneficial. however, dextrans of other size have not yet been investigated. therefore, it is unclear whether dextran 40,000 represents the optimal additive for lung preservation solutions. | 1996 | 8889986 |
| improving the palatability of oral rehydration solutions has implications for salt and water transport: a study in animal models. | it is believed that improving the taste of oral rehydration solutions (orss) might lead to greater patient acceptability. a pilot trial showed that replacing glucose with sucrose and increasing the citrate concentration at the expense of chloride improves palatability. however, the transport implications of such modifications are not known. three hypotonic experimental orss (suc/cit-ors, 211 mosmol/kg; suc/cl-ors, 224 mosmol/kg; and glu-ors, 224 mosmol/kg) were compared with a standard european ... | 1996 | 8890078 |
| studies of rat lung viability and adenine nucleotide metabolism after death. | prior studies from our laboratory have supported the use of cadaveric lungs for transplantation. in this study we investigated different preservation strategies for lungs retrieved from cadavers 4 hours after circulatory arrest. | 1996 | 8893582 |
| relative importance of prostaglandin/cyclic adenosine monophosphate and nitric oxide/cyclic guanosine monophosphate pathways in lung preservation. | modulation of vascular tone and platelet and neutrophil function through the prostaglandin/cyclic adenosine monophosphate or nitric oxide/cyclic guanosine monophosphate pathway can benefit lung graft function. the relative importance of these pathways is unclear. | 1996 | 8893590 |
| recovery of adenosine triphosphate tissue levels of grafts preserved by the two-layer method after reperfusion. | recovery of tissue adenosine triphosphate (atp) levels after transplantation is very important for graft survival. we examined whether the pancreas grafts preserved by the two-layer method have the ability to synthesize atp after reperfusion, and this is one of the mechanisms of action for the two-layer method in successful pancreas preservation. after preservation by the two-layer method using euro-collins' solution (ec) for 48 h (group 1, n = 5) or simple cold storage in ec for 48 h (group 2, ... | 1996 | 8896732 |
| comparison of bretschneider's-htk and euro-collins solution using an in vitro small bowel perfusion model. | | 1996 | 8907988 |
| nicorandil, a potent adenosine triphosphate-sensitive potassium-channel opener, ameliorates lung allograft reperfusion injury. | lung allograft ischemia-reperfusion injury, characterized by increased pulmonary vascular resistance, pulmonary edema, and hypoxia, is the most frequent cause of early graft failure. exogenous nitric oxide has been shown to reduce lung allograft reperfusion injury. during hypoxia, the adenosine triphosphate-sensitive potassium channel is an important ionic channel that links the bioenergetic metabolism to membrane excitability. it has been shown to play a critical role in vascular permeability a ... | 1996 | 8911328 |
| effects of ischaemia and preservation on the ultrastructure of the bronchiolar epithelium. a quantitative electron microscopic study of human and canine lungs. | in ten cases of clinical human single-lung transplantation, the nontransplanted euro-collins-preserved contralateral lungs were examined using electron microscopy to determine the effects of ischaemia on the bronchiolar epithelium. existing structural damage at the time of transplantation was characterized using this approach, and nine nonpreserved canine single lungs were also investigated to identify the impact of ischaemia. the study revealed a significant correlation between the duration of ... | 1996 | 8917712 |
| evaluation of four commercial enzyme immunoassays for detection of immunoglobulin m antibodies to human parvovirus b19. | four commercial enzyme immunoassays (eias) for the detection of parvovirus b19-specific immunoglobulin m (igm) antibodies [biotrin parvovirus b19 igm (biotrin international, ireland); parvoscan b19 igm (euro-diagnostica, sweden); parvovirus igm (immunobiological laboratories [ibl], germany); and human parvovirus b19 igm (hillcrest biologicals, usa)] were compared to indirect immunofluorescence assay (ifa) and polymerase chain reaction (pcr). using ifa as the reference test, high sensitivities (> ... | 1996 | 8922581 |
| effects of euro-collins, university of wisconsin, and new extracellular-type trehalase-containing kyoto solutions in an ex vivo rat lung preservation model. | we have previously reported the effects of trehalose-based extracellular-type kyoto (et-k) solution in lung preservation. now, we have developed a new et-k solution by adding three substances--n-acetyl cysteine, dibutyryl cyclic amp, and nitroglycerin, to et-k solution. we studied the effects of new et-k solution in lung preservation, and compare it with euro-collins (ec) and university of wisconsin (uw) solutions using an ex vivo rat reperfusion model. | 1996 | 8932258 |
| an effective solution for prolonged preservation of cultured human pulmonary artery endothelial cells. | pulmonary endothelium is considered the compartment most susceptible to preservation damage. this investigation was designed to analyze the efficacy of an original, university of parma low-potassium-albumin solution (spal up) on cultured human pulmonary artery endothelial cells (hpaec) and to compare its effects with those of university of wisconsin solution (uw) and euro-collins solution (ec). cryopreserved hpaec tertiary cultures were inoculated at the density of 5000 cells/cm2 in 9-cm2 well-p ... | 1996 | 8932290 |
| preservative effects of trehalose on rat muscle flaps. | in this experiment, the effect of trehalose on the preservation of rat gracilis muscle flaps was studied. the muscle flaps were preserved in 4 degrees c euro-collins solution or 4 degrees c trehalose-euro-collins solution for 6, 12, 24, 48, 72, and 96 hours, and then replanted in the same rats using microsurgical techniques. immediately after preservation and 1 week after replantation, the levels of adenosine triphosphate and creatine phosphate in flaps preserved in the trehalose-euro-collins so ... | 1996 | 8937609 |
| a bewitching case of factitious disorder in zimbabwe. | a rural zimbabwean man attributed the appearance of needles in his leg to witchcraft, but medical practitioners suspected that the needles were self-introduced. psychiatric evaluation revealed no major mental illness. the patient met criteria for the euro-american diagnosis of factitious disorder, persisting in his claim that he had been the victim of witchcraft. the patient's claim cannot be considered delusional because belief in witchcraft is culturally sanctioned within shona culture. the ca ... | 1996 | 8937912 |