| [anti-tumor effects on human laryngeal carcinoma hep-2 of recombinant fowlpox virus expressing chicken anemia virus apoptin gene]. | to investigate the anti-tumor effects and the mechanism of the recombinant fowlpox virus expressing apoptin gene on human laryngeal carcinoma hep-2. | 2009 | 19621600 |
| development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of chicken anaemia virus. | chicken anaemia virus (cav) causes an economically important viral disease in chickens worldwide. the main aim of this study was to establish a rapid, sensitive and specific loop-mediated isothermal amplification (lamp) assay for detecting cav infection. | 2010 | 19737344 |
| antitumor effects of a recombinant pseudotype baculovirus expressing apoptin in vitro and in vivo. | apoptin, a chicken anemia virus-derived, p53-independent, bcl-2-insenstive apoptotic protein with the ability to specifically induce apoptosis in tumor or transformed cells, is a promising tool for cancer gene therapy. in this study, pseudotype baculovirus, a recently developed alternative gene delivery system, was used as a vector to express apoptin. the resultant recombinant baculovirus (bv-apoptin) efficiently expressed the apoptin protein and induced apoptosis in hepg2 and h22 cells. studies ... | 2010 | 19824041 |
| proteins selectively killing tumor cells. | all human cells have a genetic program that upon activation will cause cell death, named apoptosis. cancer cells can grow due to unbalances in proliferation, cell cycle regulation and their apoptosis machinery: genomic mutations resulting in non-functional pro-apoptosis proteins or over-expression of anti-apoptosis proteins form the basis of tumor formation. surprisingly, lessons learned from viruses show that cancer cannot be regarded simply as the opposite of apoptosis. for instance, adenoviru ... | 2009 | 19836376 |
| nuclear drug delivery to target tumour cells. | cancer remains one of the leading causes of death worldwide. although enticing, the concept of a chemotherapeutic treatment directed towards a single target that kills tumour cells, without any harmful side effects or death of neighbouring cells is probably naïve, due to the fact that tumour cells arise from normal cells and share many common biological features with them. various means to damage/destroy tumour cells preferentially have been developed, but as yet, none are truly selective. howev ... | 2009 | 19836384 |
| molecular characterization of chicken infectious anemia virus circulating in argentina during 2007. | chicken infectious anemia virus (cav) is a worldwide-distributed infectious agent that affects commercial poultry. although this agent was first detected in argentina in 1994, no further studies on cav in this country were reported after that. the recent increased occurrence of clinical cases of immunosuppression that could be caused by cav has prompted this study. our results confirmed that cav is still circulating in commercial flocks in argentina. phylogenetic analysis focusing on the vp1 nuc ... | 2009 | 19848068 |
| apoptin enhances radiation-induced cell death in poorly responding head and neck squamous cell carcinoma cells. | treatment of head and neck cancers is still rather poor and worldwide new treatment options are sought. sensitizing radioresistant tumours by combining irradiation with other therapeutics to induce apoptosis are widely investigated. we examined whether chicken anaemia virus-derived apoptin protein would have a beneficial effect on irradiation of radiosensitive scc61 and radioresistant sqd9 human head and neck squamous carcinoma cell lines. in both cell lines, concurrent exposure to irradiation a ... | 2010 | 19874285 |
| pathological and immunohistochemical study of chickens with co-infection of marek's disease virus and chicken anaemia virus. | chicken anaemia virus (cav) is the most important confounding pathogen in marek's disease virus (mdv) infection. the effect of cav co-infection at 4 weeks of age after inoculation of virulent mdv (vmdv, ks strain) or very virulent mdv (vvmdv, md/5 strain) in 1-day-old chicks was investigated by pathological and immunohistochemical studies. cav increased the mortality rates induced by vmdv or vvmdv. the packed cell volume was reduced significantly in vmdv-cav infection; however, no reduction or n ... | 2009 | 19937536 |
| intestinal volvulus with coagulative hepatic necrosis in a chicken. | a 7-week-old spf chicken inoculated at 4 weeks of age with chicken anemia virus was puffed up depressed and had ruffled feathers and a good body condition. intestinal volvulus involving the jejunum and part of the duodenum forming two loops with one knob was observed. microscopically, venous infarction of the obstructed loops, periportal and sublobular multifocal coagulative hepatic necrosis and granulomatous inflammation of the cecal tonsils were observed. gram staining revealed no bacteria in ... | 2010 | 19959885 |
| characteristics of a very virulent infectious bursal disease virus from california. | an outbreak of infectious bursal disease (ibd) in two california layer flocks resulted in the isolation of two infectious bursal disease viruses designated ra and rb. increased mortality plus gross and histopathology in the layer flocks suggested ra and rb could be very virulent infectious bursal disease virus (vvibdv). preliminary studies indicated that high mortality resulted when bursa homogenates from the layer farms were used to inoculate specific-pathogen-free (spf) chicks. in addition, ra ... | 2009 | 20095162 |
| testing for viral contaminants of veterinary vaccines in hungary. | the safety of veterinary vaccines is of paramount importance and it is significantly jeopardised by extraneous agents such as bacteria, mycoplasma, chlamydia and viruses. several critical steps of vaccine manufacture involve a potential risk of viral contamination. viruses, as extraneous, agents can be divided into two main groups. group 1 agents, such as pestivirus, chicken anaemia virus (cav), and egg drop syndrome virus (edsv) are well-known to manufacturers and authorities. compendial detect ... | 2010 | 20338783 |
| development, standardization and assessment of pcr systems for purity testing of avian viral vaccines. | the european pharmacopoeia (ph. eur.) requires avian viral vaccines to be free of adventitious agents. purity testing is an essential quality requirement of immunological veterinary medicinal products (ivmps) and testing for extraneous agents includes monitoring for many different viruses. conventional virus detection methods include serology or virus culture, however, molecular tests have become a valid alternative testing method. nucleic acid testing (nat) is fast, highly sensitive and has a h ... | 2010 | 20338785 |
| an 8-year longitudinal survey for the presence of antibodies to chicken infectious anemia virus in two specific-pathogen-free strains of chickens. | cornell university maintains two genetic lines of specific-pathogen-free chickens in a filtered-air, positive-pressure house as a closed colony. offspring from each generation are maintained in the same house as the parents without clean-out between successive generations. the two lines have been persistently infected with chicken infectious anemia virus (ciav) since the mid-1990s. all flocks were monitored from 1999 to 2008 for the presence of ciav antibodies two to four times over the 65-wk li ... | 2010 | 20408398 |
| epidemiology and effect on production parameters of an outbreak of inclusion body hepatitis in broilers. | in 2007, an inclusion body hepatitis (ibh) outbreak affected several broiler farms in mississippi. results of logistic regression analyses showed significant associations between ibh occurrence and high enzyme-linked immunosorbent assay geometric mean titers for infectious bursal disease virus. however, there was no association between ibh occurrence and chicken infectious anemia virus status. results of linear regression model analyses showed significant associations between ibh occurrence with ... | 2010 | 20408402 |
| development of a loop-mediated isothermal amplification method for rapid detection of reticuloendotheliosis virus. | a loop-mediated isothermal amplification (lamp) method for rapid detection of reticuloendotheliosis virus (rev) was developed. the method used a set of two pairs of primers to amplify the pol gene for detecting rev, showing high specificity and sensitivity. the rev lamp method did not cross-react with common avian dna viruses (marek's disease virus, chicken anaemia virus, avian leucosis virus of subgroup j). additionally, the assay could detect different rev strains and had a detection limit of ... | 2010 | 20435068 |
| attenuated salmonella typhimurium carrying trail and vp3 genes inhibits the growth of gastric cancer cells in vitro and in vivo. | tumor necrosis factor-related apoptosis-inducing ligand (trail) and apoptin (vp3) of chicken anemia virus can selectively induce apoptosis in human tumor cell lines by two different pathways. salmonella not only delivers functional genes to mammalian cells but also possesses antitumor activity and therefore could be adopted as a novel vector for anticancer therapy. | 2010 | 20572589 |
| comparative susceptibility of some avian and mammalian lymphoblastoid cell lines to chicken anemia virus. | although msb1 is the most-commonly used lymphoblastoid cell line for isolation of chicken anemia virus (cav), some researchers have reported a few biological drawbacks. none of them were supported by the results of the present study. another four avian (hp1, hp2, bk3 and cu10) and two mammalian (btl-26 and ko-1) cell lines were investigated for susceptibility to the tk-5803 and ah-9409 strains. both strains caused cpe on bk3 like msb1. the mean number of positive cells for each strain in msb1 an ... | 2010 | 20683176 |
| crucial roles for protein kinase c isoforms in tumor-specific killing by apoptin. | the chicken anemia virus-derived protein apoptin induces apoptosis in a variety of human malignant and transformed cells but not in normal cells. however, the mechanisms through which apoptin achieves its selective killing effects are not well understood. we developed a lentiviral vector encoding a green fluorescent protein-apoptin fusion gene (lv-gfp-ap) that can efficiently deliver apoptin into hematopoietic cells. apoptin selectively killed the human multiple myeloma cell lines mm1.r and mm1. ... | 2010 | 20719884 |
| molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in south korea. | in south korea, 32 sequences of chicken infectious anemia virus (ciav) from various flocks of breeder and commercial chickens were genetically characterized for the first time. phylogenetic analysis of the viral protein 1 gene, including a hypervariable region of the ciav genome, indicated that korean ciav strains were separated into groups ii, iiia, and iiib. strains were commonly identified in great-grandparent and grandparent breeder farms as well as commercial chicken farms. in the field, ci ... | 2010 | 20952706 |
| development of a dna vaccine against chicken anemia virus by using a bicistronic vector expressing vp1 and vp2 proteins of cav. | in the present study, we describe the development of a dna vaccine against chicken anemia virus. the vp1 and vp2 genes of cav were amplified and cloned into pbudce4.1 to construct two dna vaccines, namely, pbudvp1 and pbudvp2-vp1. in vitro and in vivo studies showed that co-expression of vp1 with vp2 are required to induce significant levels of antibody against cav. subsequently, the vaccines were tested in 2-week-old spf chickens. chickens immunized with the dna-plasmid pbudvp2-vp1 showed posit ... | 2010 | 21146874 |
| lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus. | the acma binding domains of lactococcus lactis were used to display the vp1 protein of chicken anemia virus (cav) on lactobacillus acidophilus. one and two repeats of the cell wall binding domain of acma gene were amplified from l. lactis mg1363 genome and then inserted into co-expression vector, pbudce4.1. the vp1 gene of cav was then fused to the acma sequences and the vp2 gene was cloned into the second mcs of the same vector before transformation into escherichia coli. the expressed recombin ... | 2010 | 21181148 |
| an optimized polymerase chain reaction assay to identify avian virus vaccine contamination with chicken anemia virus. | the use of embryonating chicken eggs in preparation of avian virus vaccines is the principle cause for contamination with chicken anemia virus (cav). identification of cav in contaminated vaccines relies on the expensive, tedious, and time-consuming practice of virus isolation in lymphoblastoid cell lines. the experience of the last 2 decades indicates that polymerase chain reaction is extending to replace most of the classic methods for detection of infectious agents. in the present report, a s ... | 2011 | 21217025 |
| mortality of one-week-old chickens during naturally occurring marek's disease virus infection. | marek's disease (md) is a disease of chickens that occurs worldwide and has serious economic consequences. md can present as one of several forms, with the most commonly occurring forms being the lymphoproliferative diseases. under experimental conditions, an early mortality syndrome has been recognized following infection by some but not all strains of md virus (mdv). this is the first report of a confirmed case of mortality due to naturally occurring mdv infection in 1-week-old, nonvaccinated, ... | 2011 | 21239693 |
| production and diagnostic application of a purified, e. coli-expressed, serological-specific chicken anaemia virus antigen vp3. | the aim of this study was to evaluate the production of chicken anaemia virus vp3 protein in different escherichia coli strains and to address the diagnostic application of purified e. coli-expressed vp3 protein for the detection of chicken anaemia virus (cav) infection and the development of an elisa kit. three e. coli strains, bl21, bl21 codonplus rp and bl21 plyss, each harbouring a vp3 protein expressing plasmid, were investigated after induction to produce recombinant vp3 protein. after iso ... | 2011 | 21276209 |
| chicken anemia virus: an understanding of the in-vitro host response over time. | chicken anemia virus (cav) is an economically important virus affecting the chicken meat and egg industry. cav is characterized by anemia, lymphoid depletion, and immunosuppression. microarrays were used to investigate the response of mdcc-msb1?cells (msb1) to infection with cav at 24 and 48?h post-infection (hpi). the major genes responding to cav infection include genes involved in inflammation, apoptosis, and antiviral activity. several cytokines were differentially regulated at either 24 or ... | 2011 | 21319974 |
| apoptin gene transfer via modified wheat histone h4 facilitates apoptosis of human ovarian cancer cells. | nonviral approaches have been used extensively for intracellular gene transfer and gene therapy. a modified wheat histone h4 protein, h4tl (h4-tat-lhrh), as a protein-based gene delivery vector that was able to form stable complexes with plasmid dna and increase gene delivery efficiency has been described previously. in this study, h4tl has been used to deliver apoptin gene into a human ovarian carcinoma cell line ho8910. after transfection, increased expression of apoptin at both mrna and prote ... | 2011 | 21355783 |
| improving the potency of dna vaccine against chicken anemia virus (cav) by fusing vp1 protein of cav to marek's disease virus (mdv) type-1 vp22 protein. | studies have shown that the vp22 gene of marek's disease virus type-1 (mdv-1) has the property of movement between cells from the original cell of expression into the neighboring cells. the ability to facilitate the spreading of the linked proteins was used to improve the potency of the constructed dna vaccines against chicken anemia virus (cav). | 2011 | 21401953 |
| discovery of a genome of a distant relative of chicken anemia virus reveals a new member of the genus gyrovirus. | a 2.4-kb phi29 polymerase amplification product from serum of a diseased chicken was cloned and sequenced. the 2383-nucleotide sequence showed about 40% identity to a representative genome of chicken anemia virus (cav), the only member of the genus gyrovirus, family circoviridae. the new genome had an organization similar to that of cav: a putative 5' untranscribed region of about 400 nt followed by three partially overlapping open reading frames encoding vp1, vp2 and vp3 homologs. the amino aci ... | 2011 | 21442232 |
| molecular epidemiology of chicken anemia virus in commercial farms in china. | chicken anemia virus (cav) is the causative agent of chicken infectious anemia (cia). a high prevalence of cav has been reported in china. however, vp1 sequences of chinese isolates show no clear genotype clustering or correlation with geographic origin. therefore, the present study aimed to detect and characterize cav isolates from china based on sequence and phylogenetic analysis of the vp1, vp2 and vp3 genes. | 2011 | 21447189 |
| apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in hepg2 cells. | resistance or insensitivity to radiation therapy is one of the hallmarks of hepatocellular carcinoma. sensitizing radioresistant cancer by combining radiation with other therapeutics to induce apoptosis has been widely investigated. our previous study showed that chicken anaemia virus-derived apoptin protein induced the apoptosis of hepatic carcinoma hepg2 cells. in the present study, we demonstrated that apoptin sensitizes cells to radiation-induced apoptosis using a lentivirus-apoptin expressi ... | 2011 | 21461564 |
| application of high-resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. | fowl adenoviruses (fadvs) cause inclusion body hepatitis (ibh) in chickens. in this study, clinical cases of ibh from australian broiler flocks were screened for the presence and genotype of fadvs. | 2011 | 21495991 |
| immune complex vaccines for chicken infectious anemia virus. | infection of maternal, antibody-negative chickens with chicken infectious anemia virus (ciav) can cause clinical disease, while infection after maternal antibodies wane often results in subclinical infection and immunosuppression. currently, vaccines are not available for vaccination in ovo or in newly hatched chickens. development of ciav vaccines for in ovo use depends on the ability to generate vaccines that do not cause lesions in newly hatched chicks and that can induce an immune response r ... | 2011 | 21500642 |
| a critical role of heat shock cognate protein 70 in apoptin-induced phosphorylation of akt. | apoptin, a protein from chicken anemia virus, selectively induces apoptosis of transformed or tumor cells, but not in normal cells. however, the mechanism of action of apoptin is still not well understood. using yeast two-hybrid and immunoprecipitation approaches, we found that apoptin interacted with heat shock cognate protein 70 (hsc70). in vivo, apoptin induced the translocation of endogenous hsc70 from the cytoplasm to the nucleus, and both were co-localized in the nucleus. in addition, apop ... | 2011 | 21565169 |
| identification of the first human gyrovirus, a virus related to chicken anemia virus. | we have identified in a skin swab sample from a healthy donor a new virus that we have named human gyrovirus (hgyv), because of its similarity with the chicken anemia virus (cav), the only previously known member of the gyrovirus genus. in particular, this virus encodes an homolog of the cav apoptin, a protein that selectively induces apoptosis in cancer cells. by pcr screening, hgyv was found in 5 of 115 other non-lesional skin specimens, but in none of 92 bronchoalveolar lavages or nasopharyng ... | 2011 | 21632766 |
| high yield expression in a recombinant e. coli of a codon optimized chicken anemia virus capsid protein vp1 useful for vaccine development. | abstract: background: chicken anemia virus (cav), the causative agent chicken anemia, is the only member of the genus gyrovirus of the circoviridae family. cav is an immune suppressive virus and causes anemia, lymph organ atrophy and immunodeficiency. the production and biochemical characterization of vp1 protein and its use in a subunit vaccine or as part of a diagnostic kit would be useful to cav infection prevention. results: significantly increased expression of the recombinant full-length v ... | 2011 | 21781331 |
| gallus heat shock cognate protein 70, a novel binding partner of apoptin. | chicken anemia virus (cav) infection of newly hatched chickens induces generalized lymphoid atrophy and causes immunosuppressive. vp3, also known as apoptin, is non-structural protein of cav. apoptin specifically induces apoptosis in transformed or tumor cells but not in normal cells. in particular, there are no known cellular homologues of apoptin hindering genetic approaches to elucidate its cellular function. although a number of apoptin-interacting molecules have been identified, the molecul ... | 2011 | 21707973 |
| effects of blood sample mishandling on elisa results for infectious bronchitis virus, avian encephalomyelitis virus and chicken anaemia virus. | this study determined the effect of sample mishandling on the performance of elisas for detection of antibodies against infectious bronchitis virus (ibv), avian encephalomyelitis virus (aev) and chicken anaemia virus (cav) in the serum of chickens. the effects of five different sample mishandling treatments were assessed: heat treatment, repetitive freezing and thawing and three levels of severity of haemolysis. these mishandling treatments simulated different conditions that might occur during ... | 2011 | 22015139 |
| variants of the recently discovered avian gyrovirus 2 are detected in southern brazil and the netherlands. | a genome of a virus preliminarily named avian gyrovirus 2 (agv2), a close relative to chicken anemia virus, was recently discovered in a chicken in the state of rio grande do sul, southern brazil. to study the occurrence of agv2 in rio grande do sul and the neighboring state santa catarina, a number of adult chickens (n=108 and n=48, respectively) were tested for the presence of agv2 dna. an agv2-specific pcr was developed, optimized and used to analyze dna extracted from clinical samples. agv2 ... | 2011 | 22018524 |
| apoptin enhances the oncolytic properties of newcastle disease virus. | objective: naturally occurring strains of newcastle disease virus (ndv) have demonstrated the potential to kill cancer cells in both preclinical and clinical studies. previous studies showed that apoptin, the vp3 protein of chicken infectious anemia virus, is a p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in transformed cells. in this study, we tested the hypothesis that apoptin enhances ndv-mediated tumor cell death. methods: reverse gen ... | 2011 | 21865658 |
| analysis of chicken anemia virus genome: evidence of intersubtype recombination. | chicken anemia virus (cav) is the causative agent of chicken infectious anemia. cav putative intergenotypic recombinants have been reported previously. this fact is based on the previous classification of cav sequences into three genotypes. however, it is unknown whether intersubtype recombination occurs between the recently reported four cav genotypes and five subtypes of genome sequences. | 2011 | 22073985 |
| rapid detection of marek's disease viral genome in chicken feathers by loop mediated isothermal amplification (lamp). | a loop-mediated isothermal amplification (lamp) method for the rapid detection of serotype 1 marek's disease virus (mdv) was developed. the method used a set of three pairs of primers to amplify the meq gene for detecting serotype 1 mdv. the mdv lamp method did not cross-react with serotype 2 and serotype 3, nor did the lamp primers have binding sites for the common avian dna viruses (reticuloendotheliosis virus, chicken anemia virus, subgroup j of the avian leucosis virus). additionally, the as ... | 2011 | 22170920 |
| Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan. | Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In th ... | 2011 | 21923956 |
| Development and application of an in vitro apoptin kinase assay. | Apoptin, a protein derived from chicken anemia virus (CAV), induces apoptosis selectively in human tumor cells as compared with normal cells. This activity depends on phosphorylation and relocation of apoptin to the nucleus of cancer cells. Here, we describe an in vitro kinase assay that allows the biochemical characterization of apoptin kinase activity in tumor cells. The kinase phosphorylates apoptin in a strictly ATP-dependent fashion and in a broad salt range. The kinase activity is present ... | 2011 | 22080040 |
| dna damage response signaling triggers nuclear localization of the chicken anemia virus protein apoptin. | the chicken anemia virus (cav) protein apoptin is a small, 13.6-kda protein that has the intriguing activity of inducing g(2)/m arrest and apoptosis specifically in cancer cells by a mechanism that is independent of p53. the activity of apoptin is regulated at the level of localization. whereas apoptin is cytoplasmic in primary cells and does not affect cell growth, in transformed cells it localizes to the nucleus, where it induces apoptosis. the properties of cancer cells that are responsible f ... | 2011 | 21937663 |
| mechanisms of apoptin-induced cell death. | apoptin, a 13.6-kd protein encoded by chicken anemia virus, is paid more and more attention, since it selectively induces apoptosis in tumor cells while abolishes cytotoxic effect in normal cells. in addition, apoptin shows different localization in tumor cells and normal cells: it predominantly accumulates in nucleus of tumor cells, whereas in normal cells, it is detected mainly in cytoplasm. there are various mechanisms implicated in the program of apoptin-mediated cell death. up to now, the i ... | 2011 | 22105148 |
| inclusion body hepatitis caused by fowl adenovirus in broiler chickens in japan, 2009-2010. | from january 2009 to june 2010, many broiler chicks suddenly died without clinical signs. the mortality rates were from 1.2% to 17.0% in affected flocks. inclusion body hepatitis (ibh) was detected in 13 prefectures (northern, eastern, western, and southern areas) in japan. the livers were enlarged and pale. the bursa of fabricius and thymus had not atrophied. multifocal necroses of hepatocytes with basophilic intranuclear inclusions were seen in the liver. eosinophilic intranuclear inclusion bo ... | 2011 | 22313000 |
| flock prevalence of exposure to avian adeno-associated virus, chicken anemia virus, fowl adenovirus, and infectious bursal disease virus among ontario broiler chicken flocks. | samples from 231 randomly selected commercial broiler chicken flocks in ontario were tested at slaughter for exposure to chicken anemia virus (cav), fowl adenovirus (fadv), and infectious bursal disease virus (ibdv). fifteen blood samples per flock were collected and analyzed for the presence of antibodies against cav, fadv, and ibdv by elisa or agar gel immunodiffusion test. fifteen cecal tonsils and cloacal swabs per flock were analyzed for the presence of cav, fadv, and ibdv by pcr. the preva ... | 2014 | 24758116 |
| isolation and pathogenic analysis of virulent marek's disease virus field strain in china. | marek's disease (md) has become increasingly common in china, resulting in considerable economic loss. the etiological agent is unclear. in this study, we isolated a field md virus (mdv) strain, designated sx1301, from cvi988/rispens-vaccinated chickens with tumors. co-infection of avian leukosis virus, reticuloendotheliosis virus, and chicken infectious anemia virus was excluded by polymerase chain reaction, enzyme-linked immunosorbant assay, dna blotting hybridization, and indirect immunofluor ... | 2016 | 26976907 |
| simultaneous typing of nine avian respiratory pathogens using a novel gexp analyzer-based multiplex pcr assay. | a new, rapid, and high-throughput genomelab gene expression profiler (gexp) analyzer-based multiplex pcr method was developed for simultaneous detection and differentiation of nine avian respiratory pathogens. the respiratory pathogens included in this study were avian influenza subtypes h5, h7, and h9, infectious bronchitis virus (ibv), newcastle disease virus (ndv), infectious laryngotracheitis virus (iltv), mycoplasma gallisepticum (mg), mycoplasma synoviae (ms) and haemophilus paragallinarum ... | 2014 | 25025815 |
| detection of infectious laryngotracheitis virus by real-time pcr in naturally and experimentally infected chickens. | infectious laryngotracheitis (ilt) is an acute, highly contagious upper-respiratory infectious disease of chickens. in this study, a real-time pcr method was developed for fast and accurate detection and quantitation of iltv dna of chickens experimentally infected with iltv strain ljs09 and naturally infected chickens. the detection lower limit of the assay was 10 copies of dna. there were no cross reactions with the dna and rna of infectious bursal disease virus, chicken anemia virus, reticuloe ... | 2013 | 23840745 |
| human gyrovirus-apoptin interferes with the cell cycle and induces g2/m arrest prior to apoptosis. | the human gyrovirus-apoptin (hgyv-apoptin) is a protein that gained attention because it is selectively cytotoxic toward cancer cells. in this study, we have investigated the effect of hgyv-apoptin on cell cycle progression of cancer cells. we also compared hgyv-apoptin's action to its homologue chicken anemia virus apoptin (cav-apoptin). we show that hgyv-apoptin induces g2/m arrest in cancer cells. this is at least in part due to the fact that hgyv-apoptin induces an abnormal spindle formation ... | 2017 | 28386695 |
| genomic characterization of recent chicken anemia virus isolates in china. | chicken anemia virus (cav) causes diseases in young chickens, which include increased pathogenicity of secondary infectious agents, generalized lymphoid depletion, and immunodepression. in the present study, we have identified 22 cav strains isolated from several commercial chicken farms in northern china during 2014-2015. in addition, two cavs were also isolated from stray mouse and dog feces, respectively. to our knowledge, this is the first report of identification of cav from mouse and dog f ... | 2017 | 28344576 |
| genetic analysis of two chicken infectious anemia virus variants-related gyrovirus in stray mice and dogs: the first report in china, 2015. | chicken infectious anemia virus (ciav) causes acute viral infection in chickens worldwide. it can infect chickens of all ages, but the disease is seen only in young chickens and is characterized by hemorrhagic lesions in the muscles, atrophic changes in the lymphoid organs, aplastic bone marrow, and immunosuppression causing increased mortality. previous studies have demonstrated that ciav can be isolated from blood specimens of humans and fecal samples of stray cats. in the present study, two v ... | 2017 | 28326326 |
| c-terminal region of apoptin affects chicken anemia virus replication and virulence. | chicken anemia virus (cav) causes anemia and immune suppression, which are important diseases in the poultry industry. cav vp3, also referred as 'apoptin', has been shown to selectively kill tumor cells, raising great hopes for its utilization as an anticancer therapy. the ability of apoptin to induce apoptosis is closely related to its nuclear localization. the c-terminal region of apoptin contains a bipartite nuclear localization signals (nls), and a nuclear export signal (nes) is located betw ... | 2017 | 28222746 |
| revisiting the taxonomy of the family circoviridae: establishment of the genus cyclovirus and removal of the genus gyrovirus. | the family circoviridae contains viruses with covalently closed, circular, single-stranded dna (ssdna) genomes, including the smallest known autonomously replicating, capsid-encoding animal pathogens. members of this family are known to cause fatal diseases in birds and pigs and have been historically classified in one of two genera: circovirus, which contains avian and porcine pathogens, and gyrovirus, which includes a single species (chicken anemia virus). however, over the course of the past ... | 2017 | 28155197 |
| a truncated apoptin protein variant selectively kills cancer cells. | apoptin is a nonstructural protein encoded by one of the three open reading frames of the chicken anemia virus genome. it has attracted a great deal of interest due to its ability to induce apoptosis in multiple transformed and malignant mammalian cell lines without affecting primary and non-transformed cells. however, the use of apoptin as an anticancer drug is restricted by its strong tendency to aggregate. a number of methods to overcome this problem have been proposed, including transduction ... | 2017 | 28120180 |
| unexpected heat shock element binding ability and tumor-killing activity of the combinatorial function domain of apoptin. | apoptin, derived from the chicken anemia virus, has been found to exert tumor-preferential apoptotic activity. it is a potential anticancer agent with direct clinical applications. however, if this viral protein were to be used as a new drug, it might also induce a strong immune response, causing toxic side effects. in a previous study, our group showed that tat-apoptin downregulates the stress expression of heat shock protein 70 by competing with heat shock factor protein 1 in binding to the he ... | 2017 | 28045701 |
| genetically engineered and self-assembled oncolytic protein nanoparticles for targeted cancer therapy. | the integration of a targeted delivery with a tumour-selective agent has been considered an ideal platform for achieving high therapeutic efficacy and negligible side effects in cancer therapy. here, we present engineered protein nanoparticles comprising a tumour-selective oncolytic protein and a targeting moiety as a new format for the targeted cancer therapy. apoptin from chicken anaemia virus (cav) was used as a tumour-selective apoptotic protein. an egfr-specific repebody, which is composed ... | 2017 | 28024232 |
| full-length infectious clone of an iranian isolate of chicken anemia virus. | an iranian field strain of chicken anemia virus (cav), designated ir cav, was isolated in the marek's disease virus-transformed lymphoblastoid cell line mdcc-msb1 (msb1) culture for the first time. the full-length cav dna of this strain was cloned in the bacterial plasmid ptz57r/t to create the molecular clone ptz-cav. the nucleotide and deduced amino acid sequences of viral proteins of ir cav were compared with those of representative cav sequences including reference and commercial vaccine str ... | 2017 | 27933433 |
| molecular characterization of chicken anemia virus outbreaks in nagpur province, india from 2012 to 2015. | chicken anemia virus (cav) is one of the important poultry pathogen. cav infection can cause immunosuppression, aggravation of co-infections, vaccination failures and mortality. we are reporting the cav outbreaks from the nagpur province of india, between the years 2012-2015. the breeds included cockerel and black australorp of age varying from 29 to 50 days. the mortality rate observed among poultry was from 20% to 62.5%. clinical symptoms like anemia, subcutaneous hemorrhages, growth retardati ... | 2017 | 27899306 |
| molecular characterization of chicken infectious anemia virus from contaminated live-virus vaccines. | the aim of this study was to investigate possible causes of the pervasiveness of chicken infectious anemia virus ( ciav: ) infection in chickens in recent years in china. a total of 14 batches of live-virus vaccines were examined using pcr to detect ciav contamination, of which only 2 samples (a newcastle disease vaccine and a fowl pox vaccine) tested positive for ciav. these newcastle and fowl pox vaccines were then inoculated into 1-day-old specific-pathogen-free chickens. serum samples were c ... | 2016 | 27811322 |
| ptd4-apoptin induces bcl-2-insensitive apoptosis in human cervical carcinoma in vitro and in vivo. | worldwide, cervix carcinoma is among the most dangerous cancer types, and novel therapies are under development. cancer treatments are often hampered because of lack of specificity. the chicken anemia virus-derived apoptin induces apoptosis selectively in tumor cells and leaves normal cells unharmed. here, we have carried out in-vitro and in-vivo studies on the cytotoxic effect of apoptin in a cervix carcinoma model. apoptin was fused to the protein transduction domain 4 (ptd4), enabling deliver ... | 2016 | 27548349 |
| activation of the chicken anemia virus apoptin protein by chk1/2 phosphorylation is required for apoptotic activity and efficient viral replication. | chicken anemia virus (cav) is a single-stranded circular dna virus that carries 3 genes, the most studied of which is the gene encoding vp3, also known as apoptin. this protein has been demonstrated to specifically kill transformed cells while leaving normal cells unharmed in a manner that is independent of p53 status. although the mechanistic basis for this differential activity is unclear, it is evident that the subcellular localization of the protein is important for the difference. in normal ... | 2016 | 27512067 |
| first detection of chicken anemia virus and norovirus genogroup ii in stool of children with acute gastroenteritis in taiwan. | to date, there has been no report of co-infection of chicken anemia virus (cav) with enteric virus in patients with acute gastroenteritis (age). cav has been recently detected in various types of human samples including stool, indicating pathogenicity in gastrointestinal tract. examination by pcr-based methods of cav and norovivus genogroup ii (nv gii) in stool of 110 children with age at a hospital in taiwan revealed for the first time of co-infection in two cases. this is the first description ... | 2016 | 27405124 |
| [effect of low dose of chicken infectious anemia virus in attenuated vaccine on spf chicken body weight and vaccine immune antibody]. | in order to observe the effect of the immune and weight of chickens after use the attenuated vaccine with low dose of chicken infectious anemia virus (ciav). in this study, the effects of low dose of ciav on the weight of spf chickens and ndv antibody production were observed by simulated experiments. the results showed that 10 eid50 and 5 eid50 ciav per plume attenuated ndv vaccines were used to cause the weight loss of spf chickens. compared with the use of the non contaminated vaccine group, ... | 2016 | 27396163 |
| genomic analysis of the chicken infectious anemia virus in a specific pathogen-free chicken population in china. | the antibody to chicken infectious anemia virus (ciav) was positive in a specific pathogen-free (spf) chicken population by elisa test in our previous inspection, indicating a possible infection with ciav. in this study, blood samples collected from the spf chickens were used to isolate ciav by inoculating into msb1 cells and pcr amplification. a ciav strain (sd1403) was isolated and successfully identified. three overlapping genomic fragments were obtained by pcr amplification and sequencing. t ... | 2016 | 27298822 |
| impact of virus load on immunocytological and histopathological parameters during clinical chicken anemia virus (cav) infection in poultry. | chicken anemia virus (cav) is one the important pathogen affecting commercial poultry sector globally by causing mortality, production losses, immunosuppression, aggravating co-infections and vaccination failures. here, we describe the effects of cav load on hematological, histopathological and immunocytochemical alterations in 1-day old infected chicks. the effects of cav on cytokine expression profiles and generation of virus specific antibody titer were also studied and compared with viral cl ... | 2016 | 27165537 |
| inhibitory effect of genetically engineered mesenchymal stem cells with apoptin on hepatoma cells in vitro and in vivo. | hepatocellular carcinoma (hcc) is an aggressive tumor and has become one of the most frequent causes of cancer death in the world. the rate of post-operative recurrence and metastasis are still high even though after surgical resection. it is a difficult problem with extraordinary importance for the clinical treatment. so stem cell therapy becomes one of the anti-tumor biotherapy methods which is exploring. due to the feature of homing to tumor site and immunosuppressive, mesenchymal stem cells ... | 2016 | 27142531 |
| combined administration of the apoptin gene and poly (i:c) induces potent anti-tumor immune response and inhibits growth of mouse mammary tumors. | many viral proteins exhibit selective cytotoxicity for tumor cells without affecting the normal diploid cells. the apoptin protein of chicken infectious anemia virus is one of such proteins, which has been shown to kill tumor cells specifically. however, an effective cancer treatment strategy also requires assistance from the immune system. recently, poly (i:c) has been shown to be an effective cancer vaccine adjuvant. | 2016 | 27064544 |
| occurrence and phylogenetic studies of chicken anemia virus from polish broiler flocks. | chicken anemia virus (cav) is a widespread chicken pathogen of significant economic importance. in 2013, broiler chicken flocks in poland were examined for the presence of cav, and phylogenetic relatedness between the strains was established. ten cloacal swabs from each of 106 broiler flocks (birds aged 3-6 wk) were collected in different regions of the country and tested with the use of real-time pcr (all samples) and conventional pcr (those samples positive in real-time pcr) assays. the presen ... | 2016 | 26953947 |
| persistence of chicken anemia virus antigen and inclusions in spontaneous cases of marek's disease visceral lymphomas in broiler chickens at slaughterhouses. | the chicken anemia virus (cav) and marek's disease virus (mdv) infect chickens worldwide; a single or dual infection by these viruses has a great impact on poultry production. in the present study, we examined the existence of cav antigen and its inclusions in marek's disease (md) lymphomas in chickens in the slaughterhouses of iwate prefecture, japan. forty-nine spleens and 13 livers with different degrees of nodular lesions were histopathologically examined at our laboratory. grossly, the test ... | 2016 | 26888585 |
| serological and molecular detection of chicken anaemia virus in iranian poultry flocks. | despite chicken being the main natural host for chicken anaemia virus (cav), other birds may be infected by this virus too. in this study we examined chickens, turkeys, and quails for serological and molecular detection of cav in iran. for this study, we used 375 sera and thymus samples from broiler chickens, 100 sera and blood samples from turkeys, and 250 thymus samples from quails. the sample were collected from all over iran between 2009 and 2010. serum samples were examined using elisa. dna ... | 2015 | 26455374 |
| association of increased rate of condemnation of broiler carcasses due to hepatic abnormalities with immunosuppressive diseases in the broiler chicken industry in saskatchewan. | the objective of this study was to identify the causative agents of hepatitis observed in broiler chickens at processing. livers of chickens from 16 broiler farms in saskatchewan with gross lesions of hepatitis were collected at processing. in addition to routine bacterial isolation and histopathological examination, serologic studies for infectious bursal disease virus (ibdv) and chicken anaemia virus (cav), calculation of the ratio of the weight of the bursa of fabricius (bf) to body weight (b ... | 2015 | 26424905 |
| development of a subunit vaccine containing recombinant chicken anemia virus vp1 and pigeon ifn-γ. | chicken anemia virus (cav) is a severe threat to the chicken industry and causes heavy economic losses worldwide. in this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant vp1 (rvp1) and pigeon interferon-γ (rpiifn-γ). results indicated that rpiifn-γ enhanced humoral immunity elicited by rvp1 as early as 10 day after primary immunization and reach the high titer after secondary immunization. when compared to chickens immunized wi ... | 2015 | 26315776 |
| transcriptomic profiling of virus-host cell interactions following chicken anaemia virus (cav) infection in an in vivo model. | chicken anaemia virus (cav) is an economically important virus that targets lymphoid and erythroblastoid progenitor cells leading to immunosuppression. this study aimed to investigate the interplay between viral infection and the host's immune response to better understand the pathways that lead to cav-induced immunosuppression. to mimic vertical transmission of cav in the absence of maternally-derived antibody, day-old chicks were infected and their responses measured at various time-points pos ... | 2015 | 26244502 |
| evidence of apoptosis induced by viral protein 2 of chicken anaemia virus. | although viral protein 3 (vp3) of chicken anaemia virus (cav) has been well recognised as an inducer of apoptosis, viral protein 2 (vp2) of the virus has only been speculated to have apoptotic activity. this has not been verified because the open reading frame (orf) encoding vp2 completely encompasses that encoding vp3, and thus the possibility of expression of vp3 cannot be excluded. the aim of this study was to elucidate the potential role of vp2 as an inducer of apoptosis. site-directed mutag ... | 2015 | 26233670 |
| avian gyrovirus 2 dna in fowl from live poultry markets and in healthy humans, china. | | 2015 | 26196944 |
| t-cell reprogramming through targeted cd4-coreceptor and t-cell receptor expression on maturing thymocytes by latent circoviridae family member porcine circovirus type 2 cell infections in the thymus. | although porcine circovirus type 2 (pcv2)-associated diseases have been evaluated for known immune evasion strategies, the pathogenicity of these viruses remained concealed for decades. surprisingly, the same viruses that cause panzootics in livestock are widespread in young, unaffected animals. recently, evidence has emerged that circovirus-like viruses are also linked to complex diseases in humans, including children. we detected pcv2 genome-carrying cells in fetal pig thymi. to elucidate viru ... | 2015 | 26038767 |
| development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus. | a blocking latex agglutination test (b-lat) developed in this study was evaluated for the detection of antibodies against chicken anemia virus (cav) in chickens. polystyrene latex beads were coupled with a neutralizing monoclonal antibody (mab) to cav (mab-beads). when mab-beads were mixed with antigens prepared from the lysate of mdcc-msb1 cells infected with cav, agglutination occurred. a short pre-incubation of cav antigens with cav-specific antiserum inhibited the agglutination of mab-beads. ... | 2015 | 25952731 |
| apoptin interacts with and regulates the activity of protein kinase c beta in cancer cells. | apoptin, the vp3 protein from chicken anaemia virus (cav), induces tumour cell-specific cell death and represents a potential future anti-cancer therapeutic. in tumour but not in normal cells, apoptin is phosphorylated and translocates to the nucleus, enabling its cytotoxic activity. recently, the β isozyme of protein kinase c (pkcβ) was shown to phosphorylate apoptin in multiple myeloma cell lines. however, the exact mechanism and nature of interaction between pkcβ and apoptin remain unclear. h ... | 2015 | 25828882 |
| apoptin as a potential viral gene oncotherapeutic agent. | the use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. several viruses and their proteins have been evaluated for oncolytic effect. the vp3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while leaving normal cells unharmed. in the present study, the apoptosis inducing potential of vp3 protein of cav was evaluated in human cervical cancer cell line (hela). it was found that ... | 2015 | 25809990 |
| assessing the efficacy of an inactivated chicken anemia virus vaccine. | chicken anemia virus (cav) is an immunosuppressive virus that causes chicken infectious anemia (cia) which is a highly contagious avian disease. cav causes major economic losses in the poultry industry worldwide. the current cav vaccine is a live attenuated strain administered in the drinking water that risks horizontal infection of other chickens. the purpose of this study was to develop a novel vaccine against cav that can be administered safely using a highly pathogenic isolate inactivated wi ... | 2015 | 25758933 |
| application of cross-priming amplification (cpa) for detection of fowl adenovirus (fadv) strains. | fowl adenoviruses (fadvs) are widely distributed among chickens. detection of fadvs is mainly accomplished by virus isolation, serological assays, various polymerase chain reaction (pcr) assays, and loop-mediated isothermal amplification (lamp). to increase the diagnostic capacity of currently applied techniques, cross-priming amplification (cpa) for the detection of the fadv hexon gene was developed. the single cpa assay was optimised to detect all serotypes 1-8a-8b-11 representing the species ... | 2015 | 25655263 |
| characterization of mabs to chicken anemia virus and epitope mapping on its viral protein, vp1. | three (mocav/f2, mocav/f8 and mocav/f11) of four mouse mabs established against the a2/76 strain of chicken anemia virus (cav) showed neutralization activity. immunoprecipitation showed a band at ~50 kda in a2/76-infected cell lysates by neutralizing mabs, corresponding to the 50 kda capsid protein (vp1) of cav, and the mabs reacted with recombinant vp1 proteins expressed in cos7 cells. mocav/f2 and mocav/f8 neutralized the 14 cav strains tested, whereas mocav/f11 did not neutralize five of the ... | 2015 | 25568186 |
| chicken anemia virus and avian gyrovirus 2 as contaminants in poultry vaccines. | this study focuses on the detection of chicken anemia virus (cav) and avian gyrovirus 2 (agv2) genomes in commercially available poultry vaccines. a duplex quantitative real-time pcr (dqpcr), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. genomes of cav were detec ... | 2014 | 25439092 |
| transformed cell-specific induction of apoptosis by porcine circovirus type 1 viral protein 3. | several members of the family circoviridae have been shown to encode proteins with apoptotic activity. for example, both porcine circovirus type 2 (pcv2) and chicken anemia virus (cav) encode a third viral protein (vp3) that has been shown to be cytotoxic. interestingly, in the case of the cav protein (designated apoptin), apoptosis is specific to transformed cell types. similarities in genome structure and organization suggest that pcv type 1 (pcv1) may also contain a third orf, which codes for ... | 2015 | 25381055 |
| epidemiology and genetic characteristics of pigeon circovirus (picv) in eastern china. | pigeon circovirus (picv) is one of four viruses in the family circoviridae that affect young pigeons around the world. we collected 158 serum or tissue samples from six poultry farms in eastern china to investigate the prevalence and genetic characteristics of picv in chinese pigeons. we tested for picv using a pcr assay and found that picv was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were picv positive. one picv-p ... | 2015 | 25348272 |
| molecular characterization of chicken anemia virus circulating in chicken flocks in egypt. | introduction. although many previous studies reported detection of chicken anemia virus (cav) in egypt since 1990, genomic characterization of this circulating cav has not been published. in the present study, four nucleotide sequences of detected cav were genetically characterized. methods. these nucleotide sequences were obtained from commercial chicken flocks in two different locations of egypt during 2010. the target region for sequencing was 675 bp nucleotide of partial coding region of vp1 ... | 2014 | 25302064 |
| comparative histopathological and immunological study of two field strains of chicken anemia virus. | infection of poultry with chicken anemia virus (cav) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. recently, we have reported an increased occurrence of clinical cases caused by cav strains distantly related to those commonly used for vaccination. in order to understand the behavior of two argentinean cav strains (cav-10 and cav-18) in two-week-old chickens, an immune and h ... | 2014 | 25294222 |
| human-gyrovirus-apoptin triggers mitochondrial death pathway--nur77 is required for apoptosis triggering. | the human gyrovirus derived protein apoptin (hgv-apoptin) a homologue of the chicken anemia virus apoptin (cav-apoptin), a protein with high cancer cells selective toxicity, triggers apoptosis selectively in cancer cells. in this paper, we show that hgv-apoptin acts independently from the death receptor pathway as it induces apoptosis in similar rates in jurkat cells deficient in either fadd (fas-associated death domain) function or caspase-8 (key players of the extrinsic pathway) and their pare ... | 2014 | 25246270 |
| what is for dinner? viral metagenomics of us store bought beef, pork, and chicken. | we describe here the metagenomics-derived viral sequences detected in beef, pork, and chicken purchased from stores in san francisco. in beef we detected four previously reported viruses (two parvoviruses belonging to different genera, an anellovirus, and one circovirus-like virus) and one novel bovine polyomavirus species (bpyv2-sf) whose closest relatives infect primates. detection of porcine hokovirus in beef indicated that this parvovirus can infect both ungulate species. in pork we detected ... | 2014 | 25217712 |
| apoptins: selective anticancer agents. | therapies that selectively target cancer cells for death have been the center of intense research recently. one potential therapy may involve apoptin proteins, which are able to induce apoptosis in cancer cells leaving normal cells unharmed. apoptin was originally discovered in the chicken anemia virus (cav); however, human gyroviruses (hgyv) have recently been found that also harbor apoptin-like proteins. although the cancer cell specific activity of these apoptins appears to be well conserved, ... | 2014 | 25164066 |
| apoptin towards safe and efficient anticancer therapies. | the chicken anemia virus derived protein apoptin harbors cancer-selective cell killing characteristics, essentially based on phosphorylation-mediated nuclear transfer in cancer cells and efficient cytoplasmic degradation in normal cells. here, we describe a growing set of preclinical experiments underlying the promises of the anti-cancer potential of apoptin. various non-replicative oncolytic viral vector systems have revealed the safety and efficacy of apoptin. in addition, apoptin enhanced the ... | 2014 | 25001531 |
| novel gyroviruses, including chicken anaemia virus, in clinical and chicken samples from south africa. | introduction. chicken anaemia virus, cav, was until recently the only member of the gyrovirus genus. 6 novel gyroviruses, agv2, hgyv1, and gyv3-6, have since been discovered in human and chicken samples. methods. pcr amplification of the vp2 gene was used to detect agv2/hgyv1, gyv3, and cav in a range of clinical samples including stool, respiratory, csf, and hiv-positive plasma. screening of fresh local chicken meat was also performed. results. agv2/hgyv1 or gyv3 was detected in stools from hea ... | 2014 | 24876841 |
| identification of a chicken anemia virus variant-related gyrovirus in stray cats in china, 2012. | the chicken anemia virus (cav), is a known member of the genus gyrovirus and was first isolated from chickens in japan in 1979. some reports have also demonstrated that cav can be identified in human stool specimens. in this study, a variant of cav was detected using pcr with cav-based primers in fecal samples of stray cats. the genome of cav variant was sequenced and the results suggest that it could be a recombinant viral strain from parental cav strains jq690762 and af311900. recombination is ... | 2014 | 24689034 |
| potentiation of apoptin-induced apoptosis by cecropin b-like antibacterial peptide abps1 in human hela cervical cancer cell lines is associated with membrane pore formation and caspase-3 activation. | apoptin, a chicken anemia virus-encoded protein, induces apoptosis in chicken or human tumor cells, localizing in their nuclei as opposed to the cytoplasm of non-transformed cells. the present study was undertaken to investigate whether abps1 could potentiate apoptininduced apoptosis in hela cells. abps1 and the apoptin genes were successfully cloned into pires2-egfp expression vector and expressed in hela cells. we report that abps1 augments apoptin cell growth inhibition in a concentration- an ... | 2014 | 24633228 |
| full-genome sequence of chicken anemia virus strain gxc060821, isolated from a guangxi sanhuang chicken. | we report here the complete genomic sequence of a novel chicken anemia virus strain gxc060821, isolated from a sanhuang chicken in guangxi province of southern china. the complete genome of gxc060821 was sequenced. the full-length of gxc060821 is 2,292 bp and contains three overlapping open reading frames (orfs). a comparison of the complete sequences and the deduced amino acid sequences of gxc060821 with 31 other published chicken anemia virus sequences showed that the homologies of the nucleot ... | 2014 | 24558234 |
| phylogenetic and molecular characterization of chicken anemia virus in southern china from 2011 to 2012. | chicken anemia virus (cav) is an important pathogen that causes severe immunosuppression in young chickens. we have characterized 13 cavs isolated from different commercial farms in southern china between 2011 and 2012. we discovered 92 variable residues compared to 37 other cav complete genome sequences from other parts of the world listed in genbank; these residues have not been previously observed. all of the chinese cav genomes that were characterized in this study had a glutamine at positio ... | 2013 | 24343380 |
| λ phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo. | using phages is a novel field of cancer therapy and phage nanobioparticles (nbps) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. apoptin, a protein from chicken anemia virus (cav) has the ability to specifically induce apoptosis only in carcinoma cells. we presented a safe method of breast tumor therapy via the apoptin expressing λ nbps. here, we constructed a λ zap-cmv-apoptin recombinant nbp and investiga ... | 2013 | 24278212 |
| selective apoptosis induction in mcf-7 cell line by truncated minimal functional region of apoptin. | chicken anemia virus (cav) vp3 protein (also known as apoptin), a basic and proline-rich protein has a unique capability in inducing apoptosis in cancer cells but not in normal cells. five truncated apoptin proteins were analyzed to determine their selective ability to migrate into the nucleus of human breast adenocarcinoma mcf-7 cells for inducing apoptosis. | 2013 | 24144306 |