| characterization of the zinc finger protein encoded by the wt1 wilms' tumor locus. | we analysed the biochemical properties of the transcription factor encoded by the putative tumor-suppressor gene present at the wt1 wilms' tumor locus. a gene containing the full-length amino acid coding sequence of human wt1 was reconstructed from synthetic oligonucleotides and cloned into expression vectors for in vitro and in vivo protein synthesis. polyclonal rabbit antibodies specific for the wt1 protein were raised to an escherichia coli-produced 91 amino acid n-terminal segment and to a 1 ... | 1991 | 1662794 |
| [alterations in number of rabbit myocardial beta-adrenergic receptors in endotoxic shock: down regulation in hyperdynamic sepsis model and effects of cytokines administration]. | we investigated alterations in myocardial beta- and beta 1-adrenergic receptor (bar and b1ar) number during hyperdynamic state induced by endotoxin or cytokines. [methods] twenty-nine japanese white rabbits were divided into 2 groups. hearts were removed 18 h after intraperitoneal administration of sterile saline (sal) or e. coli endotoxin (lps; 50 micrograms/kg) (group e, n = 12), or 3 h after intravenous injection of sal or cytokines (interleukin 1-beta; 5 micrograms/kg followed by 25 ng/kg/mi ... | 1991 | 1664539 |
| the possible involvement of platelet activation in endotoxin-induced renal insufficiency in a rabbit model. | the effects of the anticoagulants, heparin and low molecular weight heparin (lmwh), and the antiplatelet agents, prostaglandin e1 (pge1) and aspirin, on endotoxin-induced renal insufficiency not induced by prerenal factors, were investigated using rabbits to evaluate the clinical usefulness of these drugs and their possible involvement in the activation of hemostasis in renal insufficiency. the intravenous administration of peg1, at 0.4 microgram/kg/min, or aspirin, at 5 mg/kg, significantly res ... | 1991 | 1667536 |
| outer membranes are competitive inhibitors of escherichia coli o157:h7 adherence to epithelial cells. | escherichia coli of serotype o157:h7 are vero cytotoxin-producing enteric pathogens that have been associated recently with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic-uremic syndrome. adherence of many enteropathogenic bacteria to mucosal surfaces is a critical step in the pathogenesis of diarrheal disease. we showed previously that adherence of e. coli o157:h7 strain cl-56 to epithelial cells in vitro is inhibited by outer membranes. in this study we examined whe ... | 1991 | 1671778 |
| pseudomonas exotoxin fusion proteins are potent immunogens for raising antibodies against p-glycoprotein. | antibodies to specific regions of human p-glycoprotein have been difficult to obtain. we developed a method to express in e. coli fusions between pseudomonas exotoxin and specific regions of human p-glycoprotein. we used the polymerase chain reaction to amplify the desired regions of mdr1 cdna and to introduce appropriate restriction sites. these fragments were cloned into the 3' end of the pseudomonas exotoxin gene. with this system we produced large amounts of fusion proteins for immunizations ... | 1991 | 1676289 |
| ultrastructural studies of the lung of turkeys (meleagris gallopavo) inoculated intratracheally with escherichia coli. | to test the hypothesis that walls of air capillaries are a site for escherichia coli to pass the air-blood barrier, fimbriated and nonfimbriated strains of e. coli were inoculated intratracheally into 18-day-old turkeys. venous blood was cultured, and turkeys were necropsied from 0.5 to 8 hours post-inoculation. lungs were processed for histopathology and electron microscopy. e. coli 078 was identified ultrastructurally using rabbit anti-lipopolysaccharide antibody and protein a-colloidal gold. ... | 1991 | 1677505 |
| characteristics of adherence of enteroaggregative escherichia coli to human and animal mucosa. | an escherichia coli strain (serotype o127a:h2) that had been isolated from a child with diarrhea in thailand and that was negative for the virulence factors of the four categories of diarrheagenic e. coli (enterotoxigenic, enteropathogenic, enteroinvasive, and enterohemorrhagic) and that showed an aggregative pattern of adherence to hela cells was investigated for adherence to native or formalin-fixed human and animal mucosa. the hemagglutinating activity and adherence ability of the bacteria we ... | 1991 | 1680107 |
| serological conservation and location of the adhesin of avian escherichia coli type 1 fimbriae. | by inoculation of mice with purified type 1-like fimbriae isolated from an avian escherichia coli strain, a monoclonal antibody (mab g5) was obtained. mab g5 reacted in an enzyme-linked immunosorbent assay (elisa) with type 1-like and type 1a fimbriae differing in the molecular masses of their major fimbrial subunit and isolated from several avian e. coli strains. the specificity of mab g5 for type 1 fimbriae was assessed in a whole bacteria elisa with 16 reference e. coli strains expressing dif ... | 1991 | 1680213 |
| dna sequences of three papa genes from uropathogenic escherichia coli strains: evidence of structural and serological conservation. | pyelonephritis-associated pili (pap) are important in the pathogenesis of ascending, unobstructive escherichia coli-caused renal infections because these surface bacterial organelles mediate digalactoside-specific binding to host uroepithelial cells. pap are composed of many different polypeptides, of which only the tip proteins mediate specific binding. the papa moiety polymerizes to form the bulk of the pilus structure and has been employed in vaccines despite its lack of gal alpha(1-4)gal rec ... | 1991 | 1682251 |
| isolation and sequence analysis of the chlamydia pneumoniae groe operon. | chlamydia pneumoniae has emerged as an important human respiratory pathogen. from a lambda gt11 gene bank constructed from c. pneumoniae isolate ar-39 dna, an immunoreactive plaque containing a 3.0-kb insert was purified. in immunoblots, a 60-kda protein was recognized by anti-c. pneumoniae rabbit immune serum. the recombinant protein was reactive with a chlamydia genus-specific monoclonal antibody recognizing a 60-kda protein found in the sarkosyl-soluble fraction and with rabbit immune serum p ... | 1991 | 1682266 |
| [antibody response in mice, rabbits and pigs in response to vaccination with an inactivated oil vaccine containing rotavirus and escherichia coli strains with k88, k99 and 987p fimbrial antigens]. | in trials with mice, rabbits and weanling piglets, four experimental charges of a combined inactivated oil vaccine against diarrhoeas in mammals were tested: the vaccine was to be implanted to sows and it contained porcine rotavirus (prv); two charges also contained bovine rotavirus and bacterins of enterotoxicogenic strains of e. coli with protective antigens k88, k99 and 987p. at low starting antibody titres the twofold i.m. implantation of 0.2 ml vaccine stimulated in mice the production of a ... | 1991 | 1686823 |
| expression of bovine leukaemia virus antigens fused to ms2 polymerase in e. coli. | the main core protein and segments of envelope proteins of bovine leukaemia virus fused to ms2 polymerase were expressed in e. coli. the synthesis rate varied between 3 and 25% of the total cellular proteins. blv-ms2 polymerase fusion proteins were detected immunologically using rabbit anti-blv sera, monoclonal antibodies and a serum of a blv-infected cow. | 1991 | 1686964 |
| discrimination by rabbit anti-idiotypic antibodies of two murine igm monoclonal antibodies directed against lipid a. | two murine igm monoclonal antibodies (mas) directed against the lipid a portion of bacterial lipopolysaccharide (lps) were compared in their binding to re lps and lipid a and their idiotypic make-up with rabbit anti-idiotypic sera. horseradish peroxidase (hrpo)-labelled mas 8-2 and 26-20 bound equally well to re lps. the binding of hrpo-labelled ma 8-2 to synthetic lipid a was low compared to the relatively strong binding of labelled 26-20. the mas proved to be competitive in a competition bindi ... | 1990 | 1694210 |
| a general strategy for polymerization, assembly and expression of epitope-carrying peptides applied to the plasmodium falciparum antigen pf155/resa. | polymerization of dna fragments in a head-to-tail arrangement provides a convenient way to obtain multimeric expression of a specific gene product, e.g., epitope-carrying peptides for immunological studies. a novel technique for the polymerization and assembly of peptides has been developed, involving the use of the class-iis restriction enzyme bspmi which enables unidirectional insertion of the dna fragments to be polymerized [kim and szybalski, gene 71 (1988) 1-8]. one or several dna fragments ... | 1990 | 1695598 |
| the expression of active human reticulocyte 15-lipoxygenase in bacteria. | a cloned cdna that encodes human reticulocyte 15-lipoxygenase was characterized by northern blot analysis and heterologous expression in bacteria. the 2.7 kb cdna specifically hybridizes to reticulocyte rna from anemic rabbits. the rna levels correlate with the appearance of enzymatic activity in anemia. the cdna was subcloned into an inducible bacterial expression vector in frame with the amino terminal ten amino acids of beta-galactosidase (puclox). the soluble fraction of the cell lysate of e ... | 1990 | 1696810 |
| anti-idiotypic antibodies counteract the invasion inhibition capacity of antibodies to major epitopes of the plasmodium falciparum antigen pf155/resa. | rabbits were immunized with the synthetic peptide eenvehda or (eenv)2, corresponding to a tandemly repeated sequence in the c-terminal part of the plasmodium falciparum antigen pf155/resa, or with escherichia coli-derived fusion proteins containing the corresponding repeats. for all sera, the capacity of the total immunoglobulin g fractions to inhibit p. falciparum merozoite invasion in vitro was similar and relatively low. affinity purification of pf155/resa-specific antibodies on parasite-infe ... | 1990 | 1696930 |
| antigenic conservation of primary structural regions of s-adenosylmethionine synthetase. | although the physical and kinetic properties of s-adenosylmethionine (adomet) synthetases from different sources are quite different, it appears that these enzymes have structurally or antigenically conserved regions as demonstrated by studies with adomet synthetase specific antibodies. polyclonal anti-human lymphocyte adomet synthetase crossreacted with enzyme from rat liver (beta isozyme), escherichia coli and yeast. in addition, polyclonal anti-e. coli enzyme and antibodies to synthetic pepti ... | 1990 | 1698095 |
| antigenic conservation of the 15,000-dalton outer membrane lipoprotein pcp of haemophilus influenzae and biologic activity of anti-pcp antisera. | a gene from haemophilus influenzae encoding an outer membrane lipoprotein of about 15,000 daltons and which comigrates with the peptidoglycan-associated lipoprotein (pal) of h. influenzae on sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been previously reported and designated pcp gene, and its product has been designated pcp. in order to obtain specific immunologic probes for the analysis of pcp expression, cellular location, and antigenic conservation in h. influenzae, pcp was f ... | 1990 | 1698180 |
| properties of a rat monoclonal antibody reactive with both the mannan of candida species and the o-antigen 6,7 polysaccharide of serogroup c1 salmonellae. | monoclonal antibody masc1-mr9 of isotype immunoglobulin m was generated in lou/c rats by immunization with heat-killed salmonella thompson (serogroup c1, o-antigen 6,7 lipopolysaccharide) bacteria which had been further enriched for o-antigen by coating with homologous lipopolysaccharide. eight monoclonal antibodies were selected by screening against the lipopolysaccharide of s. thompson. in a subsequent test against candida mannan, only one antibody, masc1-mr9, was reactive. immunofluorescence ... | 1991 | 1702760 |
| complement-mediated lysis of trypanosoma cruzi trypomastigotes by human anti-alpha-galactosyl antibodies. | antibodies that lyse trypomastigotes in a complement-mediated reaction are believed to be the main participants in the protection against virulent trypanosoma cruzi. antibodies with a specificity for alpha-galactosyl-containing determinants--generally called antigal--were studied to determine their role in the lysis of trypomastigote forms. the titers of antigal markedly increase in chagas's disease. in the present study we demonstrate binding of this antibody to t. cruzi and the complement-medi ... | 1991 | 1706399 |
| segment iv of a salmonella flagellin gene specifies flagellar antigen epitopes. | each of the two mutants isolated from a flic (= hag, flagellin-deficient) escherichia coli strain made motile by a plasmid carrying the flic gene of salmonella muenchen by selection for motility in the presence of anti-d (salmonella flagellar antigen) serum had both lost and gained one or more subfactors of the wild-type antigen. in one mutant codon 246 was gac (alanine) instead of gcc (asparagine); the other had a deletion of 105 base pairs, explicable by a 10bp direct repeat, starting at bases ... | 1991 | 1710314 |
| aromatic-dependent salmonella as live vaccine presenters of foreign epitopes as inserts in flagellin. | synthetic oligonucleotides specifying amino acid sequences identified as epitopes of various foreign antigens (cholera toxin subunit b, hepatitis b surface protein and others) have been inserted at an ecorv-ecorv deletion site in a cloned salmonella flagellin gene; the resulting plasmids, when placed in flagellin-negative escherichia coli or salmonella sp. strains, caused production of flagellin expressing the epitope. if the chimeric flagellin allowed formation of flagella, the epitope was expo ... | 1990 | 1714093 |
| expression of the f glycoprotein gene from human respiratory syncytial virus in escherichia coli: mapping of a fusion inhibiting epitope. | a cdna copy of the gene encoding the entire amino acid sequence of the fusion (f) protein of human respiratory syncytial virus (strain a2) was inserted into a bacterial expression vector containing the lambda pr promoter. upon heat induction, escherichia coli cells harboring the vector produced a 45-kda peptide which reacted with rabbit polyclonal antiserum to the native f protein. expression of the f gene resulted in severe inhibition of bacterial growth, which was overcome by deletion of the d ... | 1991 | 1714666 |
| generation and use of anti-phosphotyrosine antibodies raised against bacterially expressed abl protein. | polyclonal antibodies for phosphotyrosine can be obtained by immunization with a t/abl fusion protein. these antibodies work well in immunoblotting and immunostaining experiments to identify p-tyr proteins. for example, a 95-kda thrombin-induced p-tyr protein in platelets is recognized by these polyclonal antibodies but reacts poorly with other types of anti-p-tyr antibodies (a. golden and j.s. brugge, 1988, personal communications). to obtain large quantities of anti-p-tyr antibodies for prepar ... | 1991 | 1719348 |
| use of antibodies against the p36 protein of mycoplasma hyopneumoniae for the identification of m. hyopneumoniae strains. | mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kda protein (p36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. polyclonal antibodies were made against the recombinant p36 protein in rabbits and used for the identification of m. hyopneumoniae by the immunoblot technique. the proteins from the m. hyopneumoniae reference strains and from 13 m. hyopneumoniae field strains isolated from natur ... | 1991 | 1723490 |
| a model for the aminoacyl-trna binding site of eukaryotic elongation factor 1 alpha. | eukaryotic elongation factor 1 alpha (ef-1 alpha) binds all the aminoacyl-trnas except the initiator trna in a gtp-dependent manner. while the gtp binding site is delineated by the three gtp binding consensus elements, less is known about the aminoacyl-trna binding sites. in order to better understand this site, we have initiated cross-linking and protease mapping studies of the ef-1 alpha-gtp-aminoacyl-trna complex. two different chemical cross-linking reagents, trans-diaminedichloroplatinum(ii ... | 1992 | 1730707 |
| enhancement of bacillus calmette-guerin attachment to the urothelium by removal of the rabbit bladder mucin layer. | it has been established that the urothelial mucin layer functions as a bacterial anti-adherence factor. intravesical bacillus calmette-guerin is used to treat patients with superficial bladder cancer. the proposed mechanism of action of bacillus calmette-guerin is adherence to the urothelium with induction of an immunologic and/or inflammatory response. the current study was designed to determine if rabbit bladder mucin removal results in increased bacillus calmette-guerin urothelial adherence. ... | 1992 | 1732628 |
| marker profile, enzyme activity, and function of a human myelomonocytic leukemia cell line. | morphological and functional characteristics of a permanent human leukemia cell line (dd) that possesses myelomonocytic features were investigated. the cells bear a second type fc gamma receptor and form rosettes with sheep erythrocytes sensitized with rabbit igg (ea). however, the surface-bound ea is not internalized. the cell line lacks the surface markers cd2, cd19, cd14, hla-dr, fc gamma receptor i, fc gamma receptor iii, and cr3. alpha 1-antitrypsin, lysozyme, factor xiii a subunit of blood ... | 1992 | 1733517 |
| expression of human il-1 beta in salmonella typhimurium. a model system for the delivery of recombinant therapeutic proteins in vivo. | the feasibility of using salmonella typhimurium aroa mutant (sl3261) to deliver protein therapeutic agents was investigated in a murine model system. we have constructed an escherichia coli expression plasmid designed to express the human protein il-1 beta. this plasmid expresses il-1 beta to high levels (greater than 30% total cell protein) in e. coli. in salmonella the il-1 beta is expressed constitutively to about 10% total cell protein, as verified by western blotting analysis using polyclon ... | 1992 | 1737934 |
| [synthesis of the e-antigen of the hepatitis b virus (hbeag) in eukaryotic cells by a recombinant strain of the vaccinia virus]. | the recombinant plasmids containing the gene for hepatitis b viral core-antigen with the pre-core-sequence controlled by the early-late promoter of the 7.5' k protein gene were constructed. the recombinant strains of vaccinia virus were obtained on their basis (vhbe42-1 and vhbe42-3) selectively expressing hbeag of hepatitis b virus. the kinetics of hbeag synthesis was studied in infected cells as well as secretion of the protein into culturing medium. three proteins were found by blotting techn ... | 1991 | 1745274 |
| studies on adherence and outer membrane protein of enteropathogenic escherichia coli 0127: h6 and their related plasmids. | the following characteristics were found in 20 epidemic strains of enteropathogenic escherichia coli (epec) 0127: h6 isolated from an outbreak of neonatal diarrhea: 1) absent vero toxin production; 2) no potential for invasiveness; 3) possession of 1.5 and 60 md plasmid identical restriction digest and outer membrane protein (omp) patterns; 4) ability of localized adherence to hep-2, hela and fl cells; 5) capability to cause diarrhea in rabbits with destruction of the ileal microvilli at the are ... | 1991 | 1750334 |
| use of ampicillin-sulbactam for treatment of experimental meningitis caused by a beta-lactamase-producing strain of escherichia coli k-1. | we evaluated the pharmacokinetics and therapeutic efficacy of ampicillin combined with sulbactam in a rabbit model of meningitis due to a beta-lactamase-producing strain of escherichia coli k-1. ceftriaxone was used as a comparison drug. the mic and mbc were 32 and greater than 64 micrograms/ml (ampicillin), greater than 256 and greater than 256 micrograms/ml (sulbactam), 2.0 and 4.0 micrograms/ml (ampicillin-sulbactam [2:1 ratio, ampicillin concentration]) and 0.125 and 0.25 micrograms/ml (ceft ... | 1991 | 1759824 |
| pseudomonas aeruginosa lasb mutant constructed by insertional mutagenesis reveals elastolytic activity due to alkaline proteinase and the lasa fragment. | the extracellularly secreted endopeptidase elastase (lasb) is regarded as an important virulence factor of pseudomonas aeruginosa. it has also been implicated in the processing of lasa which enhances elastolytic activity of lasb. in order to investigate the role of lasb in virulence and lasa processing, a lasb-negative mutant, pao1e, was constructed by insertional mutagenesis of the lasb structural gene, lasb, in p. aeruginosa pao. an internal 636 bp lasb fragment of the plasmid prb1803 was liga ... | 1991 | 1766384 |
| a recombinant escherichia coli heat-stable enterotoxin (sta) fusion protein eliciting anti-sta neutralizing antibodies. | a recombinant fusion protein consisting of native escherichia coli heat-stable enterotoxin (sta) and a dimer of a synthetic igg-binding fragment (zz), derived from staphylococcus aureus protein a was produced in e. coli. the fusion protein (zzsta) was secreted in large quantities into the growth medium and recovered by affinity chromatography on igg-sepharose. rabbits immunized with the fusion protein responded by producing high serum levels of anti-sta antibodies that also effectively neutraliz ... | 1991 | 1769524 |
| enterotoxigenic klebsiella pneumoniae in acute childhood diarrhoea. | k. pneumoniae strains recovered as pure or predominant isolate from stool specimens of 50 children below three years of age, presenting with acute watery diarrhoea, were studied for heat-labile enterotoxin production. twenty three (46%) of the 50 crude toxin concentrates showed positivity in rabbit ileal loops and skin permeability tests. antigenically 17 (34%) and 20 (40%) of the toxin extracts reacted with immuno-purified anti h-lt antibody in latex particle agglutination and immuno-dot blot a ... | 1991 | 1778616 |
| translocation of bacteria from gut lumen to mesenteric lymph nodes--and beyond? | | 1991 | 1779320 |
| [cloning of the gene and immunochemical specificity of recombinant immunoglobulin binding protein v7 from streptococcus valente (group g)]. | the fragment of the structural gene coding for the fc-receptor of streptococcus valente (g group) has been cloned. the resulting recombinant plasmid ppgsv1 contains the o, kb hindiii fragment of streptococcal chromosomal dna inserted into the vector plasmid puc19 and determines the expression of the 31 kd protein in escherichia coli cells. the protein binds the immunoglobulins of human, rabbit, guinea pig origin, but in contrast to the g protein of another g group streptococcus it is nonreactive ... | 1991 | 1784298 |
| cross immunoreactivity of aortic kappa-2-elastin with some antibacterial antisera. | immunological and microbial factors may lead to the formation of atherosclerotic lesions. cross immune reactions between aortic kappa-2-elastin and antisera against some antigens of gram-negative bacteria--salmonella and escherichia coli--were studied using the dot-immunobinding assay. positive results were obtained with antisera against salmonella ao, bo, co, eo, hm and escherichia coli ok(a). among immunological mechanisms leading to atherosclerosis cross immunoreactivity of aortic antigens wi ... | 1991 | 1797575 |
| [an immunoenzyme test system for the detection of antibodies to campylobacter]. | an enzyme immunoassay system for the detection of antibodies to bacteria of the genus campylobacter in human blood serum has been developed. the system is based on the use of ethanol-treated c. jejuni and c. coli whole cells as antigen. the study of sera obtained from healthy donors in this assay has made it possible to establish the value of the tentative diagnostic titer: 320. | 1991 | 1801490 |
| the effect of human milk on the adherence of enterohemorrhagic e. coli to rabbit intestinal cells. | | 1991 | 1808994 |
| [a comparative study of bacteroides fragilis and e. coli related to the pathogenesis of calcium bilirubinate gallstones]. | a comparative study of bacteroides fragilis and e. coli bacterial infection in the biliary tract in relation to the pathogenesis of pigment stone formation was carried out on the basis of gallstone rabbit's model of anaerobic bacterial infection. one hundred and twenty japanese hybrid big-ear white rabbits were randomly divided into four groups: 14 in control group, 31 in b. fragilis (bf) group, 42 in e. coli group and 33 in the mixed group. in the experimental groups we successfully made gallst ... | 1991 | 1814825 |
| high-level bacterial expression, purification and characterization of human calreticulin. | to investigate its cellular function and role in autoimmune disease pathogenesis, we have bacterially expressed human calreticulin, a major calcium-binding protein in the endoplasmic reticulum and a human autoantigen. this is the first report describing the heterologous expression of calreticulin from any source. the recombinant calreticulin constituted approximately 32% of the soluble escherichia coli proteins, and was purified to apparent homogeneity by ion exchange and hydrophobic liquid chro ... | 1991 | 1817262 |
| rapid method for altering bacterial ribosome-binding sequences for overexpression of proteins in escherichia coli. | in an escherichia coli expression system, two genes, one from an anaerobic intestinal bacterium and one from e. coli, were overexpressed following the alteration of ribosome-binding (shine-dalgarno) sequences. for both genes, the polymerase chain reaction (pcr) was used to modify the ribosome-binding sequence and, at the same time, provide restriction endonuclease sequences at each end of the gene. these restriction endonuclease sequences were used for inserting the dna into the e. coli plasmid ... | 1991 | 1821779 |
| lung vascular injury after administration of viable hemolysin-forming escherichia coli in isolated rabbit lungs. | escherichia coli hemolysin, a transmembrane pore-forming exotoxin, is considered an important virulence factor. in the present study, the possible significance of hemolysin production was investigated in a model of septic lung failure through infusion of viable bacteria in isolated rabbit lungs; 10(4) to 10(7) e. coli/ml perfusate caused a dose- and time-dependent appearance of hemolysin, accompanied by release of potassium, thromboxane a2, and pgi2 into the perfusate. concomitantly, marked pulm ... | 1991 | 1826193 |
| [hemolytic properties of escherichia coli strains isolated from cases of urinary tract infection and enteropathy]. | different strains of e. coli, that were isolated during years 1986-1990 from 217 urine samples in patients with asymptomatic bacteriuria, cystitis, cysto-pyelitis and pyelonephritis, appeared to be more frequently haemolytic (34.5%) than strains of e. coli isolated from faecal samples in patients with acute enteropathy. it was confirmed that the haemolysin production occurs during log -phase of bacterial growth. the degree of haemolysis can vary widely according to the strain involved. agglutina ... | 1991 | 1844919 |
| immunologic priming with recombinant hepatitis a virus capsid proteins produced in escherichia coli. | hepatitis a virus capsid proteins (vp0, vp3, and vp1) have been synthesized in escherichia coli for use in antigenic and immunogenic analyses. rabbits immunized with each of these individual recombinant capsid proteins developed a rapid neutralizing antibody response when subsequently challenged with a subimmunogenic dose of whole virus. | 1991 | 1850041 |
| autoantibodies to dna topoisomerase ii in juvenile rheumatoid arthritis. | sera from 58 children with juvenile rheumatoid arthritis were examined for the presence of antibodies to dna topoisomerase ii. eight sera were reactive in immunoblotting with purified human topoisomerase ii and a protein encoded by a cloned cdna expressed in escherichia coli which represents the carboxy-terminal domain of the human enzyme. in addition, the sera detect topoisomerase ii in mitotic chromosomes and chromosome scaffolds. five of the sera bind to the native enzyme in solution and depl ... | 1991 | 1851057 |
| [the pyrogenicity of lipopolysaccharide-contaminated human albumin solutions]. | | 1991 | 1852766 |
| temperature-sensitive production of rabbit muscle glycogen phosphorylase in escherichia coli. | in order to understand how allosteric switches regulate both the catalytic activity and molecular interactions of glycogen phosphorylase, it is necessary to design and analyze variant proteins that test hypotheses about the structural details of the allosteric mechanism. essential to such an investigation is the ability to obtain large amounts of variant proteins. we developed a system for obtaining milligram amounts (greater than 20 mg/l) of rabbit muscle phosphorylase from bacteria. phosphoryl ... | 1991 | 1857717 |
| interaction of ribo- and deoxyriboanalogs of yeast trna(phe) anticodon arm with programmed small ribosomal subunits of escherichia coli and rabbit liver. | a synthetic ribooligonucleotide, r(ccagacugm-aagaucugg), corresponding to the unmodified yeast trna(phe) anticodon arm is shown to bind to poly(u) programmed small ribosomal subunits of both e. coli and rabbit liver with affinity two order less than that of a natural anticodon arm. its deoxyriboanalogs d(ccagactgaagatctgg) and d(ccaga)r(cugm-aaga)d(tctgg), are used to study the influence of sugar-phosphate modification on the interaction of trna with programmed small ribosomal subunits. the deox ... | 1991 | 1870974 |
| stepwise improvements in catalytic effectiveness: independence and interdependence in combinations of point mutations of a sluggish triosephosphate isomerase. | second-site suppressor changes that improve the catalytic potency of a sluggish mutant of the enzyme triosephosphate isomerase have been examined both individually and in combination. each of the second-site mutations increases the specific catalytic activity of a triosephosphate isomerase in which the catalytic base, glutamate-165, has been changed to aspartate. these second-site suppressors are g10s, s96p, s96t, e97d, v167d, and g233r. not one of these changes enhances the value of kcat/km for ... | 1991 | 1883832 |
| fever produced by intrahypothalamic injection of interleukin-1 and interleukin-6. | pure human interleukin-1 beta (il-1 beta) and interleukin-6 (il-6), both of natural origin, were found to cause fever in rabbits when injected into the po/ah region of the brain. the threshold dose required for this effect was between 0.4 and 4 u, equivalent to 0.04 to 0.4 ng for il-1 beta, and around 50 u, equivalent to 0.05 ng for il-6. from this it was estimated that this area of the brain responds to a local concentration of approximately 1 ng/ml of these cytokines, a level which can easily ... | 1991 | 1883958 |
| characterization of thermostable direct hemolysins encoded by four representative tdh genes of vibrio parahaemolyticus. | four tdh genes encoding thermostable direct hemolysin (tdh) cloned from two representative strains of v. parahaemolyticus (tdh 1 and tdh 2 from a hemolytic strain, tdh3 and tdh4 from a non-hemolytic strain) have different nucleotide sequences (the maximum divergence: 3.3%). in this study, each tdh gene product was purified from the lysate of escherichia coli cells carrying the cloned gene and their properties were compared to investigate the influence of the amino acid substitutions caused by th ... | 1991 | 1890953 |
| [sodium sulfapyridazine in new delayed-action bases for the treatment of bacterial conjunctivitis]. | possible preparation of 10 per cent sodium sulfapyridazine ophthalmic drops containing aubazidan, a microbial polysaccharide, providing prolongation of the drops action and stability on the storage was studied. the pharmacokinetic studies showed that aubazidan which is a natural polymer provided high prolongation of the sulfapyridazine effect in the tissues of the anterior part of the eye in rabbits. the prolonged effect was similar to the previously observed effect of the solutions containing s ... | 1991 | 1892429 |
| sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes. | a sensitive enzyme immunoassay for measurements of rat parvalbumin was established using antibodies raised in rabbits with parvalbumin purified from skeletal muscles. antibodies in the antiserum were purified with a parvalbumin-coupled sepharose column. the sandwich-type immunoassay system for parvalbumin was composed of polystyrene balls with immobilized purified antibodies and the same antibodies labeled with beta-d-galactosidase from escherichia coli. the assay was highly sensitive and the mi ... | 1991 | 1892868 |
| efficacy of enteric-coated protease in preventing attachment of enterotoxigenic escherichia coli and diarrheal disease in the ritard model. | in this study, we report on a novel approach based on modification of the intestinal surface to prevent diarrhea caused by enterotoxigenic escherichia coli (etec). the removable intestinal tie adult rabbit diarrhea (ritard) model was used to test the efficacy of an enteric-coated protease preparation (detach; enzacor technology pty. ltd.) in the prevention of bacterial attachment and diarrheal disease caused by colonization factor antigen i-positive (cfa/i+) e. coli h10407. protease was administ ... | 1991 | 1894370 |
| hsp104 is a highly conserved protein with two essential nucleotide-binding sites. | most eukaryotic cells produce proteins with relative molecular masses in the range of 100,000 to 110,000 after exposure to high temperatures. these proteins have been studied only in yeast and mammalian cells. in saccharomyces cerevisiae, heat-shock protein hsp104 is vital for tolerance to heat, ethanol and other stresses. the mammalian hsp110 protein is nucleolar and redistributes with growth state, nutritional conditions and heat shock. the relationships between hsp110, hsp104 and the high mol ... | 1991 | 1896074 |
| molecular cloning of the thymus-specific parvalbumin known as avian thymic hormone: isolation of a full length cdna and expression of the recombinant protein in escherichia coli. | the complete coding sequence of the thymus-specific parvalbumin called avian thymic hormone (ath) has been cloned into escherichia coli. the translated amino acid sequence was found to be identical to the sequence of map turtle parvalbumin at 90 of 108 positions. northern blot analysis of thymic rna indicated a transcript length of approximately 1050 bp. however, the ath cdna probe failed to hybridize to poly(a)+ rna from chicken leg muscle, a further indication that avian thymic hormone is dist ... | 1991 | 1897928 |
| purification and characterization of catalytically active precursor of rat liver mitochondrial aldehyde dehydrogenase expressed in escherichia coli. | the cdna coding for the precursor (p-aldh) or mature (m-aldh) rat liver mitochondrial aldehyde dehydrogenase was cloned in an expression vector pt7-7 and expressed in escherichia coli strain bl21 (de3)/plyss. the p-aldh expressed in e. coli was a soluble tetrameric protein. it exhibited virtually the same specific activity and kms for substrates as m-aldh. n-terminal sequencing of isolated p-aldh provided the evidence that the catalytic activity was not derived from a partially processed mature- ... | 1991 | 1898068 |
| b subunit of cholera toxin produced in escherichia coli. | an engineered e. coli strain containing high expression level of ct-b subunits has been obtained by the application of recombinant dna techniques. the b subunit can be secreted into the medium and reaches 20-40 micrograms/ml when this strain is incubated in a 50 l fermentation tank. the ct-b subunit purified with affinity chromatography in e. coli has the same characters as the natural ct-b subunit in molecular weight, n terminal amino acid analysis and antigenicity. the ct-b subunit has good im ... | 1991 | 1902353 |
| the carboxyl-terminal region of staphylococcal enterotoxin type a is required for a fully active molecule. | staphylococcal enterotoxin type a (sea) gene (sea+) mutations were constructed by exonuclease iii digestion or cassette mutagenesis. five different sea mutations that had 1, 3, 7, 39, and 65 codons deleted from the 3' end of sea+ were identified and confirmed by restriction enzyme and nucleotide sequence analyses. each of these sea mutations was constructed in escherichia coli and transferred to staphylococcus aureus by using the plasmid vector pc194. culture supernatants from the parent s. aure ... | 1991 | 1903773 |
| the smg gds-induced activation of smg p21 is initiated by cyclic amp-dependent protein kinase-catalyzed phosphorylation of smg p21. | we have shown that smg p21b is phosphorylated by cyclic amp-dependent protein kinase (protein kinase a) and that membrane acidic phospholipids such as phosphatidic acid and phosphatidylinositol markedly inhibit the smg gds-induced activation of smg p21b. however, we show here that phosphatidic acid and phosphatidylinositol exhibit a less inhibitory effect on the smg gds-induced activation of the phosphorylated form of smg p21b. thus, in the presence of membrane acidic phospholipids, the smg gds- ... | 1991 | 1905539 |
| cytochrome b5 and a recombinant protein containing the cytochrome b5 hydrophobic domain spontaneously associate with the plasma membranes of cells. | both cytochrome b5, isolated from rabbit liver microsomes, and lacz:hp, a recombinant protein consisting of enzymatically active escherichia coli beta-galactosidase coupled to the c-terminal membrane-anchoring hydrophobic domain of cytochrome b5, were shown to spontaneously associate with the plasma membranes of erythrocytes and 3t3 cells. association was promoted by low ph values, but proceeded satisfactorily over several hours at physiological ph and temperature. about 150,000 cytochrome b5 mo ... | 1991 | 1906748 |
| effect of polymyxin b on experimental shock from meningococcal and escherichia coli endotoxins. | meningococcemia is the most frequent cause of septic shock in healthy children. to determine whether polymyxin b (pmb) might improve mortality from meningococcal shock, its protective activity was evaluated in rabbits challenged with an ld90 of meningococcal lipooligosaccharide (los) and compared with an ld80 of escherichia coli o111:b4 lipopolysaccharide (lps). pmb (5 mg/kg) administered intravenously 30 min before meningococcal los challenge had no significant effect on heart rate, mean arteri ... | 1991 | 1908001 |
| functional evidence that the ser-195 residue of staphylococcal exfoliative toxin a is essential for biological activity. | the substitution of the serine 195 residue of staphylococcal exfoliative toxin a by a cysteine residue led to a biologically inactive protein. this result is consistent with the hypothesis that exfoliative toxin a could be a protease or a lipase. however, no protease or lipase activity was detected with the native toxin. | 1991 | 1908832 |
| bacteriologically stressed animal model of new closed catheter drainage system with microbicidal outlet tube. | we have used a catheterized rabbit model to test a newly developed catheter drainage system that incorporates a microbicidal outlet tube. in these tests, this new system was compared with a currently marketed closed drainage system, with daily challenge at the outlet tube using an auxotrophically marked uropathogenic strain of escherichia coli. in the control series (silicone catheter connected to a standard drainage bag via a tamper-evidence seal) the challenge organism developed upstream biofi ... | 1991 | 1909475 |
| pyrogenic responses of the pika, ochotona rufescens rufescens. | in 3-month-old male and female pikas, ochotona rufescens rufescens, reared at an ambient temperature of 25 degrees c, the mean within-day body temperature was 39.2 degrees c without significant variance. at an ambient temperature of 35 degrees c it increased markedly in males while decreased slightly at 5 degrees c. the pikas showed lower sensitivity than japanese white rabbits to a pyrogenic e. coli endotoxin. | 1991 | 1915603 |
| effect of the microbial constituents, lps and bcg, on the glycosaminoglycans of chondrocyte cultures. | earlier we reported that articular chondrocytes in monolayer culture produce pericellular proteoglycans both with short and long half lives, t-1 and t-2 (kittlick et al. 1991 b). now monolayer cultures have been investigated to assess the influence on the metabolism of pericellular proteoglycans or glycosaminoglycans by lipopolysaccharide of e. coli and s. typhimurium as well as by bacillus calmette-guérin. | 1991 | 1915758 |
| fine mapping of canine parvovirus b cell epitopes. | in this report we describe the topological mapping of neutralizing domains of canine parvovirus (cpv). we obtained 11 cpv-specific monoclonal antibodies (mabs), six of which are neutralizing. the reactivities were as determined by elisa and western blot (immunoblot) analysis. vp2, the most abundant protein of the cpv capsid, seemed to contain all the neutralization sites. also, an almost full-length genomic clone of cpv was constructed in the bacterial plasmid puc18 to enable expression of cpv p ... | 1991 | 1919526 |
| [the mechanism of corneal ring formation caused by bacterial endotoxin]. | corneal ring can be produced experimentally by injection of endotoxin into the rabbit cornea. to clarify this phenomenon, the effects of inhibitors of arachidonic acid metabolite were studied on the formation of the corneal ring. phospholipase a2 inhibitor (dexamethasone) and lipoxygenase inhibitor (tei-3308) blocked the accumulation of polymorphonuclear leukocytes (pmns) in the endotoxin-injected cornea, while cyclooxygenase inhibitor (indomethacin) enhanced pmn accumulation. this result sugges ... | 1991 | 1927741 |
| t7 infection-dependent selective expression of cloned genes in p1-lysogenic escherichia coli. | expression systems based on the selective transcription of genes cloned behind a t7 promoter, by t7 rna polymerase, display a non-negligible basal expression when the t7 rna polymerase gene is present within the host organism before induction of the system. this is a problem, especially for cloning and controlled expression of genes toxic to the host organism. we have circumvented this problem by taking advantage of abortive t7 infection of e. coli (p1), in the course of which t7 rna polymerase ... | 1991 | 1930165 |
| fluorescence study of a mutant cytochrome b5 with a single tryptophan in the membrane-binding domain. | fluorescence studies of cytochrome b5 are complicated by the presence of three tryptophans, at positions 108, 109, and 112, in the membrane-binding domain. the cdna for rabbit liver cytochrome b5, isolated from a lambda gt11 library, was used to generate a mutated mrna where the codons for tryptophans-108 and -112 were replaced by codons for leucine. the sequence was expressed in escherichia coli and the mutant protein was isolated. this mutant protein had the expected absorption spectrum, and i ... | 1991 | 1931948 |
| characterisation of the binding sites for escherichia coli heat-labile toxin type i in intestinal brush borders. | intestinal brush borders from wistar rats contained a total of 20-30-times more binding sites for escherichia coli heat-labile enterotoxin (lt-1) than for cholera toxin (ct). the results suggest that lt-1 binds to sites in addition to ganglioside gm1, the binding site for ct. brush border proteins were separated by sds-page, blotted to nitrocellulose and the filters incubated with 125i-labeled toxins. [125i]lt-1 was shown to bind to a series of brush border galactoproteins ranging in size from 1 ... | 1991 | 1932071 |
| immunoreactive alpha a crystallin in rat non-lenticular tissues detected with a sensitive immunoassay method. | for the quantitative analysis of the a subunit of alpha crystallin (alpha a) in the lens and for the survey of possible existence of alpha a in the non-lenticular tissues, we have established a highly sensitive and specific immunoassay method for alpha a. antisera to alpha a were raised in rabbits with alpha a purified from bovine lens, or the c-terminal decapeptide (eekpssapss) of alpha a (alpha apep). the antibodies to alpha a and alpha apep were purified by the use of an alpha a-coupled sepha ... | 1991 | 1932094 |
| the serological relationship between yersinia enterocolitica o9 and escherichia coli o157 using sera from patients with yersiniosis and haemolytic uraemic syndrome. | sera from patients with yersiniosis, shown to contain antibodies to yersinia enterocolitica o9; and sera from patients with haemolytic uraemic syndrome (hus) caused by escherichia coli o157, were used to investigate serological cross-reactions between y. enterocolitica o9 and e. coli o157. lipopolysaccharide (lps) was isolated from strains of y. enterocolitica o9 and e. coli o157 and reacted with sera by immunoblotting and elisa. sera from patients with hus contained antibodies to the lps of e. ... | 1991 | 1936156 |
| recombinant escherichia coli clones expressing chlamydia trachomatis gene products attach to human endometrial epithelial cells. | to identify chlamydia trachomatis genes involved in attachment to host cells, a chlamydial genomic library was screened on the basis of binding characteristics by two methods. in the whole-cell screen, individual recombinant escherichia coli clones were assayed for adherence to eukaryotic cells. in the membrane-binding screen, each recombinant colony of e. coli was treated with chcl3 and assayed for binding to purified, 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propanesulfonate (chaps)-solubili ... | 1991 | 1937759 |
| immunization of guinea pigs with recombinant tmpb antigen induces protection against challenge infection with treponema pallidum nichols. | treponema pallidum-susceptible guinea pigs of strain c4d were immunized with recombinant t. pallidum antigens tmpa, tmpb, tmpc, and tmpa plus tmpb plus tmpc; with escherichia coli membranes; or with adjuvant alone. animals in groups of five received six immunizing injections, each of 100 micrograms of antigen incorporated in ribi adjuvant. after the sixth immunization, all experimental and nonimmunized controls were intradermally challenged with 3 x 10(6) t. pallidum nichols freshly extracted fr ... | 1991 | 1937794 |
| secretory reflexes in ileum and jejunum: absence of remote effects. | we have tested the hypothesis that luminal secretagogues initiate neural reflexes that alter ion transport in small intestinal segments proximal or distal to the site of the secretory stimulus. effects of secretagogues that act by different mechanisms were studied in vitro by measuring short circuit current (isc) of ileum or jejunum mounted in a unique flux chamber while proximal mucosa in neural continuity with the tissue was perfused with secretagogues (na deoxycholate. escherichia coli sta, 5 ... | 1991 | 1940027 |
| the localization of indium-111-leukocytes, gallium-67-polyclonal igg and other radioactive agents in acute focal inflammatory lesions. | a variety of radioactive agents, injected directly intravenously have demonstrated foci of inflammation by gamma camera imaging, avoiding the in vitro preparation of labeled leukocytes. this study sought to find out if any of these agents mimicked the biodistribution in abscesses and non-target organs of labeled mixed leukocyte suspensions. eight different agents were compared with 111in-oxine labeled leukocytes in an acute soft tissue e. coli abscess and an acute arthritic lesion in 24 dogs one ... | 1991 | 1941149 |
| synthesis of bovine leukemia virus antigens in escherichia coli. | plasmids were constructed by the use of pex vectors that encode and express different parts of the bovine leukemia virus (blv): main core protein p24, nucleic acid-binding protein p12, transmembrane protein gp30, and different segments of envelope protein gp51. expression of fusion proteins with molecular weights higher than 117 kd for all recombinant plasmids was shown in coomassie-blue stained gels and by western blot analysis with rabbit anti-blv sera. coupling of a gp51-encoding with a p24-e ... | 1990 | 1966363 |
| identification of plasmid-encoded mannose-resistant hemagglutinin and hep-2 and hela cell adherence factors of two diarrheagenic escherichia coli strains belonging to an enteropathogenic serogroup. | two escherichia coli strains (b/m 369 and c-35) belonging to enteropathogenic serogroup o86 were isolated from patients with infantile diarrhea and studied with respect to their cellular adherence properties. both strains exhibited adherence (ad+) to hep-2 and hela cell monolayers in vitro and expressed mannose-resistant hemagglutinating (mrha+) activity towards human, chicken, and sheep (but not mouse, rabbit, or guinea pig) erythrocytes. cellular adherence properties of both strains could be s ... | 1990 | 1969390 |
| cloning of the genes for af/r1 pili from rabbit enteroadherent escherichia coli rdec-1 and dna sequence of the major structural subunit. | af/r1 pili on the surface of escherichia coli rdec-1 promote attachment of the bacteria to rabbit intestinal brush borders. in order to characterize af/r1 pili and manipulate their expression, we cloned the genes necessary for af/r1 expression; determined the size of proteins produced in minicells; located the gene encoding the major structural subunit, named afra; and determined the dna sequence of afra as well as the sequence of 700 additional nucleotides upstream of afra. two contiguous ecori ... | 1990 | 1969392 |
| streptococcus pneumoniae-stimulated macrophages induce neutrophils to emigrate by a cd18-independent mechanism of adherence. | neutrophil adherence to and emigration across endothelium are in large part dependent upon the neutrophil membrane cd11/cd18 glycoprotein complex. recently, however, we have demonstrated that some stimuli can elicit neutrophil emigration in the lung by a cd18-independent pathway. we examined further the mechanism involved in cd18-independent emigration in a rabbit model of inflamed peritoneum. neutrophil emigration in the peritoneum induced by instillation of e. coli and s. pneumoniae was studie ... | 1990 | 1972663 |
| expression of tac antigen component of bovine interleukin-2 receptor in different leukocyte populations infected with theileria parva or theileria annulata. | the tac antigen component of the bovine interleukin-2 receptor was expressed as a cro-beta-galactosidase fusion protein in escherichia coli and used to raise antibodies in rabbits. these antibodies were used for flow cytofluorimetric analysis to investigate the expression of tac antigen in a variety of theileria parva-infected cell lines and also in three theileria annulata-infected cell lines. cells expressing tac antigen on their surface were found in all t. parva-infected cell lines tested wh ... | 1990 | 1979317 |
| immune complex induced experimental colitis: beneficial effect of sulphasalazine in rabbits. | experimental colitis was induced in rabbits by exposing the colon mucosa to 1% formalin followed by i.v. injections of soluble immune complexes made with antigen in excess. the animals were preimmunized with escherichia coli o14:k7:h--inducing antibodies cross-reactive to intestinal epithelium. animals with this colitis were divided in two groups. one group was treated with sulphasalazine and the other was given vehicle only. sulphasalazine was administered daily at 125.5 mumol (50 mg) per kg bo ... | 1990 | 1983323 |
| construction and expression of the complete clostridium difficile toxin a gene in escherichia coli. | cloned fragments constituting the 8.1-kb toxin a gene of clostridium difficile were used to reconstruct the intact gene. the recombinant toxin expressed in escherichia coli was cytotoxic, enterotoxic, and lethal. in addition, toxic lysate caused hemagglutination of rabbit erythrocytes. the toxic activities were inhibited by antibody specific for toxin a. our findings demonstrate that the biological activities exhibited by native toxin a are functions of a single protein encoded by the 8.1-kb tox ... | 1991 | 1987027 |
| contribution of a 28-kilodalton membrane protein to the virulence of haemophilus influenzae. | a haemophilus influenzae b (hib) membrane protein with a molecular mass of 28 kda bound polyclonal antisera raised against a highly purified hib fimbrial subunit. we cloned the gene encoding this protein and found that the gene was expressed in escherichia coli. dna sequence analysis identified an 843-bp open reading frame which predicted a 26.78-kda protein with an amino-terminal signal sequence and a mature protein with 70% similarity to the 28-kda lipoprotein of e. coli (f. yu, s. inouye, and ... | 1991 | 1987077 |
| trat: a powerful carrier molecule for the stimulation of immune responses to protein and peptide antigens. | a number of integral membrane proteins (imps) isolated from escherichia coli have been examined for their ability to generate serum antibody responses in the absence of adjuvant. these proteins were found to stimulate high titers of serum antibody when injected into rabbits or mice in saline. the antibody titers elicited were not significantly increased by the addition of a powerful adjuvant such as ifa. covalent conjugation of bsa, of the dnp group, and of a peptide ag from plasmodium falciparu ... | 1991 | 1988496 |
| identification of three amino acid residues in the b subunit of shiga toxin and shiga-like toxin type ii that are essential for holotoxin activity. | shiga toxin of shigella dysenteriae type i and shiga-like toxins i and ii (slt-i and slt-ii, respectively) of enterohemorrhagic escherichia coli are functionally similar protein cytotoxins. these toxin molecules have a bipartite molecular structure which consists of an enzymatically active a subunit that inhibits protein synthesis in eukaryotic cells and an oligomeric b subunit that binds to globotriaosylceramide glycolipid receptors on eukaryotic cells. regionally directed chemical mutagenesis ... | 1991 | 1991714 |
| identification of residues essential for progesterone binding to uteroglobin by site-directed mutagenesis. | in order to identify amino acids directly involved in progesterone binding to rabbit uteroglobin we have mutated phe 6, tyr 21 and thr 60 by site-directed mutagenesis of the uteroglobin cdna. these residues have been postulated previously to participate in progesterone binding. high-level expression of the mutated uteroglobin cdnas in escherichia coli yields recombinant protein mutants that, like natural uteroglobin, form stable dimers, suggesting that the tertiary structure of the protein has n ... | 1991 | 1997121 |
| cloning of trichosanthin cdna and its expression in escherichia coli. | several cdna clones coding for trichosanthin (tcs) have been isolated from a cdna library prepared from the poly(a)+rna of the root tuber of trichosanthes kirilowii maximowicz. the nucleotide sequence codes for a protein of 289 amino acids (aa) including a putative signal peptide of 23 aa and an extra 19 aa at the c terminus; the latter two have not been found in tcs obtained from the natural product [collins et al., j. biol. chem. 265 (1990) 8665-8669]. recombinant tcs (retcs) was synthesized i ... | 1991 | 1999291 |
| the effect of postnatal age on the adherence of enterohemorrhagic escherichia coli to rabbit intestinal cells. | enterohemorrhagic escherichia coli (ehec) are associated with hemorrhagic colitis and hemolytic uremic syndrome. these illnesses are typically seen in young children, but are rare before 6 mo of age. the cause of this age restriction is unclear. because bacterial adherence to intestinal mucosa is considered a critical initial event in pathogenesis, we studied the ontogeny of the adherence of ehec (o157:h7 and other serotypes) isolated from children with diarrhea, hemorrhagic colitis, or hemolyti ... | 1991 | 2000256 |
| a recombinant c-terminal toxin fragment provides evidence that membrane insertion is important for clostridium perfringens enterotoxin cytotoxicity. | clostridium perfringens enterotoxin (cpe) is believed to be involved in several important gastrointestinal illnesses. recent studies have identified a number of distinct molecular events which occur after cpe treatment of eukaryotic cells or isolated membranes. additional studies are underway to determine the temporal order and intrinsic importance of each cpe event for cytotoxicity. we now demonstrate that a truncated cpe fragment binds to membranes, but is unable to insert into membranes or ca ... | 1991 | 2014001 |
| pharmacokinetics and antibacterial efficacy of cefpirome (hr 810) in experimental escherichia coli and haemophilus influenzae type b meningitis. | cefpirome (hr 810) is a new cephalosporin related to cefotaxime that has potent bactericidal activity against a broad spectrum of gram-negative and gram-positive organisms. the pharmacokinetics and bacteriological efficacy of cefpirome administered as a single intravenous dose were assessed in rabbits with experimental haemophilus influenzae type b and escherichia coli k1 meningitis. the mean penetrations into the cerebrospinal fluid (csf) in relation to the amount of drug in serum of animals in ... | 1991 | 2024953 |
| production of antisera selective for m1 muscarinic receptors using fusion proteins: distribution of m1 receptors in rat brain. | a fragment of the cdna encoding the third intracellular loop of the rat m1 muscarinic receptor was cloned, and the dna was expressed in escherichia coli as a fusion protein. the fusion protein was purified and utilized as an antigen to raise a polyclonal antiserum in rabbits. chinese hamster ovary cells stably transfected with the cdna encoding each of the five known subtypes of muscarinic receptor were used as tissue sources to test the antiserum. the antiserum was found to quantitatively immun ... | 1991 | 2034236 |
| r plasmid in escherichia coli o103 coding for colonization of the rabbit intestinal tract. | one rabbit pathogenic escherichia coli strain, belonging to serogroup o103, harbors a self-transferable 117-kb plasmid (prec-1) encoding resistance to several antibiotics. the role of this r plasmid in the colonization of the digestive tract in specific-pathogen-free (e. coli o103-free) rabbits was studied. five-week-old rabbits were inoculated with the wild-type strain, with its variant cured of the plasmid, with an e. coli k-12 strain, or with an untypeable e. coli strain from a healthy rabbit ... | 1991 | 2037350 |
| biosynthesis of klebsiella k2 capsular polysaccharide in escherichia coli hb101 requires the functions of rmpa and the chromosomal cps gene cluster of the virulent strain klebsiella pneumoniae chedid (o1:k2). | the genes determining the biosynthesis of type 2 (k2) capsular polysaccharide [3----beta glc1,4----beta man(1,3----beta glcua) 1,4----alpha glc1----] of klebsiella pneumoniae chedid (o1:k2), which is highly virulent for mice, were cloned and introduced into escherichia coli hb101 and into four noncapsulated mutants derived from k. pneumoniae reference strains of k1, k7, k9, and k28. the recombinant plasmid pcps7b06 carried 23 kb of a chromosomal dna fragment of strain chedid and encoded a part o ... | 1991 | 2037364 |
| the c terminus of mouse ornithine decarboxylase confers rapid degradation on dihydrofolate reductase. support for the pest hypothesis. | several years ago, we proposed that polypeptide regions rich in proline (p), glutamic acid (e), serine (s), and threonine (t) (pest) target intracellular proteins for destruction (rogers, s., wells, r., and rechsteiner, m. (1986) science 234, 364-368). to test the pest hypothesis, we have produced chimeric proteins in which the n or c terminus of mouse dihydrofolate reductase is extended by the pest-containing c terminus of mouse ornithine decarboxylase. oligonucleotides encoding the 37 c-termin ... | 1991 | 2040628 |
| infection of rabbit mammary glands with ovine mastitis bacterial strains. | an experimental model was developed in rabbits to study ovine mastitis. a total of 19 ovine mastitis bacterial strains (seven staphylococcus aureus, four staph. chromogenes, four staph. hyicus and four escherichia coli) were used for mammary gland infections. the histopathological results showed that the ovine mastitis types corresponded to experimental infections produced in the rabbit with the ovine strains. these results helped the grading of the bacterial species tested according to the seve ... | 1991 | 2061429 |
| molecular cloning of aldolases for synthetic applications. | | 1990 | 2076016 |