| immunogenic burkholderia pseudomallei outer membrane proteins as potential candidate vaccine targets. | burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. there is no vaccine towards the bacterium available in the market, and the efficacy of many of the bacterium's surface and secreted proteins are currently being evaluated as vaccine candidates. | 2009 | 19654871 |
| ecology and characterization of polyhydroxyalkanoate-producing microorganisms on and in plants. | polyhydroxyalkanoates are energy reserve polymers produced by bacteria to survive periods of starvation in natural habitats. little is known about the ecology of polyhydroxyalkanoate-producing bacteria. to analyse the occurrence of this specific group on/in seven different plant species, a combined strategy containing culture-dependent and -independent methods was applied. using microbial fingerprint techniques (single-strand conformation polymorphism analysis with specific primers for phac gene ... | 2009 | 19656194 |
| burkholderia cepacia keratitis with endophthalmitis. | keratitis with endophthalmitis of the right eye occurred in a 78-year-female following a complicated cataract surgery. prompt intravitreal vancomycin and ceftazidime with topical fortified tobramycin and cefazolin treatments were started. the corneal, aqueous and vitreous cultures grew a burkholderia cepacia complex (bcc) strain on the fourth day. restriction fragment length polymorphism analysis of the reca amplicon revealed b. cepacia genomovar i. the organism was found to be susceptible to ce ... | 2009 | 19661206 |
| [twenty years of care for cystic fibrosis adults in czech republic]. | most of cystic fibrosis (cf) patients survive now into adulthood and they are transferred to pulmonologist care. | 2009 | 19662884 |
| a burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens. | understanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. to provide insight into this area, we fabricated a protein microarray containing 1,205 burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, and identified 170 reactive antigens. this subset of antigens was printed on a smaller array and probed with a collection of 747 individual sera derived from 10 patient groups including melioidosis ... | 2009 | 19666533 |
| burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of vietnam. | nitrogen-fixing bacteria, strains sa41(t), sa42 and sa53, were isolated from an aquatic plant, eleocharis dulcis, that grows in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam. the isolates were gram-negative, aerobic, non-spore-forming, rod-shaped bacteria, having a cell width of 0.6-0.7 microm and a length of 1.5-1.7 microm. they showed good growth between ph 3.0 and 7.0, and between 17 and 37 degrees c. the organisms contained ubiquinone q-8 as the predominant isopr ... | 2010 | 19666801 |
| burkholderia cenocepacia: a new biocatalyst for efficient bioreduction of ezetimibe intermediate. | ezetimibe is a selective acyl-coenzyme a: cholesterol acyltransferase (acat) inhibitor used in hypercholesterolemia. synthesis of ezetimibe requires enantiopure 3-[5-(4-fluorophenyl)-5(s)-hydroxypentanoyl]-4(s)-4-phenyl-1,3-oxazolidin-2-one (fop alcohol) as a crucial intermediate which is produced by reduction of the corresponding prochiral ketone (fop dione). a new biocatalyst from acclimatized soil was screened for bioreduction of the above prochiral ketone. the microorganism was identified by ... | 2009 | 19669185 |
| environmentally relevant parameters affecting pcb degradation: carbon source- and growth phase-mitigated effects of the expression of the biphenyl pathway and associated genes in burkholderia xenovorans lb400. | the principal means for microbial degradation of polychlorinated biphenyls (pcbs) is through the biphenyl pathway. although molecular aspects of the regulation of the biphenyl pathway have been studied, information on environmental facets such as the effect of alternative carbon sources on (polychlorinated) biphenyl degradation is limited. here we explore the effect of environmental conditions (e.g., carbon source and growth phase) on the variation in pcb degradation profiles of burkholderia xen ... | 2010 | 19672561 |
| occidiofungin, a unique antifungal glycopeptide produced by a strain of burkholderia contaminans. | bacterial strain burkholderia contaminans ms14 was isolated from soil that suppressed brown patch disease of lawn grass. an antifungal compound was purified from the liquid culture of this bacterium. in this study, complete covalent structures of two purified closely related antifungal compounds were determined by the experiments of tocsy, noesy, roesy, 13c hsqc 2d nmr, and esi-ms and gc. the analysis of monoisotopic masses of the purified preparation indicated the presence of two related compou ... | 2009 | 19673482 |
| cystic fibrosis infections: treatment strategies and prospects. | pseudomonas aeruginosa and burkholderia cepacia are the two major gram-negative rods that colonize/infect the lungs of patients with cystic fibrosis (cf). these organisms may cause progressive respiratory failure, although occasionally more rapid infections result in the 'cepacia' syndrome. many antibiotics have been used against pseudomonas and burkholderia, but once chronic colonization has been established, eradication of these organisms is rare. drug therapy for cf patients is compromised by ... | 2009 | 19674113 |
| has the era of untreatable infections arrived? | antibiotic resistance is a major public health concern, with fears expressed that we shortly will run out of antibiotics. in reality, the picture is more mixed, improving against some pathogens but worsening against others. against methicillin-resistant staphylococcus aureus (mrsa)--the highest profile pathogen--the range of treatment options is expanding, with daptomycin, linezolid and tigecycline all launched, and telavancin, ceftobiprole, ceftaroline and dalbavancin anticipated. there is a gr ... | 2009 | 19675016 |
| a diagnosis of burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. | melioidosis is an infectious disease caused by the gram-negative bacterium burkholderia pseudomallei. interest in the molecular identification of b. pseudomallei has increased after its classification as a category b agent by the us centers for disease control and prevention. the present article reports a diagnosis of b. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. the results obtained show that direct detection of the 16-23s spacer sequence in br ... | 2009 | 19679240 |
| antibacterial activities of a fosfomycin/tobramycin combination: a novel inhaled antibiotic for bronchiectasis. | to compare the in vitro and in vivo activities of a 4:1 (w/w) fosfomycin/tobramycin combination (fti) with those of fosfomycin and tobramycin alone against cystic fibrosis (cf) and non-cf bronchiectasis pathogens. | 2009 | 19679597 |
| structural, evolutionary and genetic analysis of the histidine biosynthetic "core" in the genus burkholderia. | in this work a detailed analysis of the structure, the expression and the organization of his genes belonging to the core of histidine biosynthesis (hisbhaf) in 40 newly determined and 13 available sequences of burkholderia strains was carried out. data obtained revealed a strong conservation of the structure and organization of these genes through the entire genus. the phylogenetic analysis showed the monophyletic origin of this gene cluster and indicated that it did not undergo horizontal gene ... | 2009 | 19683039 |
| structural and biological diversity of lipopolysaccharides from burkholderia pseudomallei and burkholderia thailandensis. | burkholderia pseudomallei, the etiological agent of melioidosis, is a facultative intracellular pathogen. as b. pseudomallei is a gram-negative bacterium, its outer membrane contains lipopolysaccharide (lps) molecules, which have been shown to have low-level immunological activities in vitro. in this study, the biological activities of b. pseudomallei lps were compared to those of burkholderia thailandensis lps, and it was found that both murine and human macrophages produced levels of tumor nec ... | 2009 | 19692625 |
| the genus burkholderia: analysis of 56 genomic sequences. | the genus burkholderia consists of a number of very diverse species, both in terms of lifestyle (which varies from category b pathogens to apathogenic soil bacteria and plant colonizers) and their genetic contents. we have used 56 publicly available genomes to explore the genomic diversity within this genus, including genome sequences that are not completely finished, but are available from the ncbi database. defining the pan- and core genomes of species results in insights in the conserved and ... | 2009 | 19696499 |
| effects of repeated glyphosate applications on soil microbial community composition and the mineralization of glyphosate. | repeated applications may have a greater impact on the soil microbial community than a single application of glyphosate. experiments were conducted to study the effect of one, two, three, four or five applications of glyphosate on soil microbial community composition and glyphosate mineralization and distribution of (14)c residues in soil. | 2010 | 19697445 |
| versatile dual-technology system for markerless allele replacement in burkholderia pseudomallei. | burkholderia pseudomallei is the etiologic agent of melioidosis, a rare but serious tropical disease. in the united states, genetic research with this select agent bacterium is strictly regulated. although several select agent compliant methods have been developed for allelic replacement, all of them suffer from some drawbacks, such as a need for specific host backgrounds or use of minimal media. here we describe a versatile select agent compliant allele replacement system for b. pseudomallei ba ... | 2009 | 19700544 |
| acc (1-aminocyclopropane-1-carboxylate) deaminase activity, a widespread trait in burkholderia species, and its growth-promoting effect on tomato plants. | the genus burkholderia includes pathogens of plants and animals and some human opportunistic pathogens, such as the burkholderia cepacia complex (bcc), but most species are nonpathogenic, plant associated, and rhizospheric or endophytic. since rhizobacteria expressing acc (1-aminocyclopropane-1-carboxylate) deaminase may enhance plant growth by lowering plant ethylene levels, in this work we investigated the presence of acc deaminase activity and the acds gene in 45 strains, most of which are pl ... | 2009 | 19700546 |
| characterisation of bacteria from pinus sylvestris-suillus luteus mycorrhizas and their effects on root-fungus interactions and plant growth. | bacteria from pinus sylvestris-suillus luteus mycorrhizas were isolated, characterised, and their effects on p. sylvestris-s. luteus interactions and plant growth investigated in vitro. the isolates formed five distinct phenotypic and physiological groups. two of the groups, accounting for 34 of the 55 isolates, consisted of bacillus spp., with three subgroups represented. the other groups contained burkholderia spp., serratia spp. and pseudomonas spp. representatives from each bacterial group w ... | 2002 | 19709201 |
| bacterial populations associated with mycelium of the arbuscular mycorrhizal fungus glomus intraradices. | abstract the influences of the arbuscular mycorrhizal fungus glomus intraradices on the culturable aerobic-heterotrophic bacterial communities in the rhizosphere and hyphosphere of cucumber plants (cucumis satvius) were investigated. mycorrhizal and non-mycorrhizal plants were grown in compartmentalised growth units, each with a root compartment and two lateral root-free compartments. samples representing rhizosphere, root-free soil, root-free sand and washed sand extract were collected 52 days ... | 2002 | 19709247 |
| phase variation has a role in burkholderia ambifaria niche adaptation. | members of the burkholderia cepacia complex (bcc), such as b. ambifaria, are effective biocontrol strains, for instance, as plant growth-promoting bacteria; however, bcc isolates can also cause severe respiratory infections in people suffering from cystic fibrosis (cf). no distinction is known between isolates from environmental and human origins, suggesting that the natural environment is a potential source of infectious bcc species. while investigating the presence and role of phase variation ... | 2010 | 19710710 |
| endophytic nitrogen fixation in dune grasses (ammophila arenaria and elymus mollis) from oregon. | several tropical grasses harbor symbiotic nitrogen-fixing bacteria within their stem and rhizome tissue that may contribute to the nitrogen nutrition of the host plant. we present evidence here that sand dune grasses (ammophila arenaria and elymus mollis) from oregon also contain nitrogen-fixing bacteria. surface-sterilized stem and rhizome tissue from these species possess acetylene reduction (nitrogen fixation) activity and large populations (10(5) to 10(6) cfu/g fresh weight) of bacteria. the ... | 2004 | 19712295 |
| burkholderia cenocepacia o antigen lipopolysaccharide prevents phagocytosis by macrophages and adhesion to epithelial cells. | chronic respiratory infections by the burkholderia cepacia complex (bcc) are of great concern to patients with cystic fibrosis. bcc isolates may survive intracellularly within amoebae, respiratory epithelial cells and macrophages. the molecular mechanisms facilitating colonization and pathogenesis remain unclear. given the importance of bacterial adhesion to host surfaces in microbial pathogenesis, we investigated the role of the o antigen lps in the interaction of burkholderia cenocepacia, a me ... | 2009 | 19713359 |
| a novel isoquinoline alkaloid, dd-carboxypeptidase inhibitor, with antibacterial activity isolated from streptomyces sp. 8812. part i: taxonomy, fermentation, isolation and biological activities. | a novel isoquinoline alkaloid of molecular formula c10h9no4, labeled js-1, was isolated from the culture broth of streptomyces sp. 8812. it was purified by acetone protein precipitation from the culture supernatant, followed by anion exchange and c18 rp hplc columns. js-1 is an inhibitor of exocellular dd-carboxypeptidases/transpeptidases (dd-peptidases) 64-575 ii from saccharopolyspora erythraea 64-575 ii, and r39 from actinomadura r39. js-1 exhibits activity against gram-negative bacteria, suc ... | 2009 | 19713994 |
| [persistence of burkholderia cenocepacia bacteria in vivo in dependence of their ability to form biofilms]. | to select the most susceptible line of mice which allows to conduct comparative studies of infectious process caused by different strains of b. cepacia in order to explore correlation between ability to form biofilms and persistence of bacteria in organs of infected animals. | 2009 | 19715200 |
| [role of "quorum sensing" regulatory system in formation of biofilms by burkholderia cepacia and pseudomonas aeruginosa]. | to study influence of chemically synthesized lactones with different length of carbonic chain on formation of biofilms by burkholderia cepacia and pseudomonas aeruginosa. | 2009 | 19715201 |
| epidemiology and molecular characterization of a clone of burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a french intensive care unit. | clustered cases of nosocomial pulmonary infections were observed in a french intensive care unit. biochemical tests showed the etiologic agents to be part of the bcc (bcc). reca polymerase chain reaction-restriction fragment length polymorphism analysis and molecular phylogeny positioned the isolates into burkholderia cenocepacia. their reca sequences were found identical to those of et12 strains responsible of necrotic pneumonia in cystic fibrosis patients. analyses of a multi locus sequence ty ... | 2010 | 19716254 |
| a rhizospheric burkholderia cepacia complex population: genotypic and phenotypic diversity of burkholderia cenocepacia and burkholderia ambifaria. | the burkholderia cepacia'complex' (bcc) presently comprises nine species and genomovars. in order to acquire a better comprehension of the species and genomovar distribution and of the genetic diversity among environmental bcc bacteria, a natural population of 60 bacterial isolates recovered from the rhizosphere of maize and belonging to the bcc has been characterised to assess the exact taxonomic position, the genetic polymorphism and the metabolic profiles of isolates. the identification of th ... | 2003 | 19719571 |
| degradation of mixtures of aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbon-degrading bacteria. | the well-characterized toluene-oxidizing bacteria pseudomonas putida paw1, p. putida f1, p. mendocina kr1, burkholderia cepacia g4, b. cepacia js150, and ralstonia pickettii pko1, as well as several strains of bacteria isolated from activated sludge, were examined for their ability to degrade mixtures of aromatic and chloroaliphatic hydrocarbons. collectively, the strains tested were able to transform all of the 14 aromatic hydrocarbons included in the mixtures, with most strains degrading five ... | 2003 | 19719688 |
| polymorphisms within the prnd and pltc genes from pyrrolnitrin and pyoluteorin-producing pseudomonas and burkholderia spp. | abstract pyrrolnitrin (prn) and pyoluteorin (plt) are broad-spectrum antibiotics produced by several strains of pseudomonas and burkholderia species. both antibiotics play an important role in the suppression of multiple plant pathogenic fungi. primers were developed from conserved sequences and amplified prnd and pltc fragments from 18 pseudomonas and four burkholderia spp. of worldwide origin that produce either prn or plt or both. subsequent rflp (restriction fragment length polymorphisms) an ... | 2003 | 19719693 |
| prevalence-dependent use of serological tests for diagnosing glanders in horses. | the internationally mandatory complement fixation test (cft) for testing of equine sera for the absence of glanders has repeatedly led to discrepant results. not only do "false positive" sera pose a problem for the diagnostician and the animal health authorities but they can also result in significant financial losses for the animal owners.due to the very low prevalence of glanders in the horse population it is of major importance to use tests with a high specificity to overcome unreliable predi ... | 2009 | 19723336 |
| identification of burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody. | the genus burkholderia includes many bacteria that cause serious human infections. as is the case with other gram-negative bacteria, burkholderia species produce lps, which is an abundant component of the bacterial cell surface. burkholderia cepacia complex (bcc) bacteria (which include at least 17 separate species) produce lps structures that are quite different. in an attempt to determine the degree of lps epitope variation among bcc species, a mab was produced, designated 5d8, specific for th ... | 2010 | 19729457 |
| isolation of identical nitrilase genes from multiple bacterial strains and real-time pcr detection of the genes from soils provides evidence of horizontal gene transfer. | bacterial enzymes capable of nitrile hydrolysis have significant industrial potential. microbacterium sp. aj115, rhodococcus erythropolis aj270 and aj300 were isolated from the same location in england and harbour identical nitrile hydratase/amidase gene clusters. strain aj270 has been well studied due to its nitrile hydratase and amidase activity. r. erythropolis itcbp was isolated from denmark and carries a very similar nitrile hydratase/amidase gene cluster. in this study, an identical nitril ... | 2009 | 19730817 |
| effect of two types of biosurfactants on phenanthrene availability to the bacterial bioreporter burkholderia sartisoli strain rp037. | biosurfactants are tensio-active agents that have often been proposed as a means to enhance the aqueous solubility of hydrophobic organic contaminants, such as polycyclic aromatic hydrocarbons (pahs). biosurfactant-producing bacteria such as those belonging to the genus pseudomonas might therefore enhance pah availability to pah-degrading bacteria. we tested the effects of two types of biosurfactants produced by pseudomonas sp., cyclic lipopeptides and rhamnolipids, on phenanthrene bioavailabili ... | 2010 | 19730847 |
| aerobic biotransformation of polybrominated diphenyl ethers (pbdes) by bacterial isolates. | polybrominated diphenyl ethers (pbdes) are flame retardants that have been used in consumer products and furniture for three decades. currently, very little is known about their fate in the environment and specifically about their susceptibility to aerobic biotransformation. here, we investigated the ability of the polychlorinated biphenyl (pcb) degrading bacteria rhodococcus jostii rha1 and burkholderia xenovorans lb400 to transform mono- through hexa-bdes at ppb levels. we also tested the pbde ... | 2009 | 19731666 |
| the molecular and cellular basis of pathogenesis in melioidosis: how does burkholderia pseudomallei cause disease? | melioidosis, a febrile illness with disease states ranging from acute pneumonia or septicaemia to chronic abscesses, was first documented by whitmore & krishnaswami (1912). the causative agent, burkholderia pseudomallei, was subsequently identified as a motile, gram-negative bacillus, which is principally an environmental saprophyte. melioidosis has become an increasingly important disease in endemic areas such as northern thailand and australia (currie et al., 2000). this health burden, plus th ... | 2009 | 19732156 |
| reca gene sequence and multilocus sequence typing for species-level resolution of burkholderia cepacia complex isolates. | to identify, by means of reca sequencing and multilocus sequence typing (mlst), burkholderia cepacia complex (bcc) isolates of environmental and clinical origin, which failed to be identified by reca rflp and species-specific pcr. | 2009 | 19732325 |
| extended spectrum beta-lactamases among gram-negative bacteria from an egyptian pediatric hospital: a two-year experience. | knowledge of the anti-microbial susceptibility pattern of common pathogens in a given area helps to inform the choice of empirical antibiotic therapy. the aim of this study was to determine the existence and to describe the characteristics of extended spectrum beta-lactamase (esbls) in a pediatric hospital in mansoura university, egypt, to aid in the choice of empirical antibiotic therapy. | 2007 | 19734604 |
| cervical osteomyelitis caused by burkholderia cepacia after rhinoplasty. | burkholderia cepacia, previously known as pseudomonas cepacia, has been implicated in vertebral osteomyelitis in patients who are intravenous drug abusers. we report a case of acute vertebral osteomyelitis in a non-intravenous drug user, following an elective rhinoplasty. | 2008 | 19736393 |
| melioidosis in south-eastern queensland. | | 2009 | 19740059 |
| trends in pathogens colonising the respiratory tract of adult patients with cystic fibrosis, 1985-2005. | the treatment of patients with cf has continued to evolve. we hypothesised that sputum microbiology may have changed as a result of this. | 2009 | 19740710 |
| eurocarecf quality assessment of diagnostic microbiology of cystic fibrosis isolates. | the identification of microbial species from respiratory specimens and their susceptibility to antimicrobial agents are among the most important diagnostic measures of care for patients with cystic fibrosis (cf). under the umbrella of eurocarecf, two quality assurance trials of cf microbiology were performed in 2007 and 2008. nine formulations with cf bacterial isolates were dispatched. a total of 31/37 laboratories from 18/21 european countries participated in the 2007 and 2008 trials. the comm ... | 2009 | 19741077 |
| two type iv secretion systems with different functions in burkholderia cenocepacia k56-2. | bacterial type iv secretion systems (t4ss) perform two fundamental functions related to pathogenesis: the delivery of effector molecules to eukaryotic target cells, and genetic exchange. two t4sss have been identified in burkholderia cenocepacia k56-2, a representative of the et12 lineage of the b. cepacia complex (bcc). the plant tissue watersoaking (ptw) t4ss encoded on a resident 92 kb plasmid is a chimera composed of virb/d4 and f-specific subunits, and is responsible for the translocation o ... | 2009 | 19744991 |
| role for the burkholderia pseudomallei capsular polysaccharide encoded by the wcb operon in acute disseminated melioidosis. | the capsular polysaccharide of burkholderia pseudomallei is an essential virulence determinant that is required for protection from host serum cidal activity and opsonophagocytosis. in this study, the immune response directed against a b. pseudomallei capsule mutant (jw270) was investigated in an acute respiratory murine model. jw270 was significantly attenuated in this model ( approximately 2 logs) to levels resembling those of avirulent burkholderia thailandensis. at lethal doses, jw270 coloni ... | 2009 | 19752033 |
| functional characterization of an orphan cupin protein from burkholderia xenovorans reveals a mononuclear nonheme fe2+-dependent oxygenase that cleaves beta-diketones. | cupins constitute a large and widespread superfamily of beta-barrel proteins in which a mononuclear metal site is both a conserved feature of the structure and a source of functional diversity. metal-binding residues are contributed from two core motifs that provide the signature for the superfamily. on the basis of conservation of this two-motif structure, we have identified an orf in the genome of burkholderia xenovorans that encodes a novel cupin protein (bxe_a2876) of unknown function. recom ... | 2009 | 19754880 |
| nosocomial burkholderia cepacia infections in a turkish university hospital: a five-year surveillance. | burkholderia cepacia has the potential to cause fatal infections in icus, and multidrug resistance makes them a serious threat in hospital settings. the aim of this study was to evaluate the epidemiology of b. cepacia infections in our hospital. | 2009 | 19759490 |
| burkholderia cepacia complex: impact on the cystic fibrosis lung lesion. | burkholderia cepacia complex (bcc) is a group of phenotypically similar nonfermenting, aerobic, gram-negative rods that infect 2 to 8% of patients with cystic fibrosis (cf). bcc comprises several distinct species (formerly termed genomovars). infection with bcc has been associated with worse outcomes and survival in cf patients. additionally, colonization with bcc has been associated with heightened mortality following lung transplantation. currently, the role of lung transplantation in bcc-infe ... | 2009 | 19760547 |
| assessment of three resistance-nodulation-cell division drug efflux transporters of burkholderia cenocepacia in intrinsic antibiotic resistance. | burkholderia cenocepacia are opportunistic gram-negative bacteria that can cause chronic pulmonary infections in patients with cystic fibrosis. these bacteria demonstrate a high-level of intrinsic antibiotic resistance to most clinically useful antibiotics complicating treatment. we previously identified 14 genes encoding putative resistance-nodulation-cell division (rnd) efflux pumps in the genome of b. cenocepacia j2315, but the contribution of these pumps to the intrinsic drug resistance of t ... | 2009 | 19761586 |
| reciprocal regulation by the cepir and cciir quorum sensing systems in burkholderia cenocepacia. | burkholderia cenocepacia belongs to a group of closely related organisms called the b. cepacia complex (bcc) which are important opportunistic human pathogens. b. cenocepacia utilizes a mechanism of cell-cell communication called quorum sensing to control gene expression including genes involved in virulence. the b. cenocepacia quorum sensing network includes the cepir and cciir regulatory systems. | 2009 | 19761612 |
| comparison of the bacterial community and characterization of plant growth-promoting rhizobacteria from different genotypes of chrysopogon zizanioides (l.) roberty (vetiver) rhizospheres. | molecular approaches [pcr-denaturing gradient gel electrophoresis (dgge)] were used to determine whether three different vetiver (chrysopogon zizanioides) genotypes, commercially used in brazil and considered economically important over the world, select specific bacterial populations to coexist in their rhizospheres. dgge profiles revealed that the predominant rhizospheric bacterial community hardly varies regarding the vetiver genotype. moreover, using traditional cultivation methods, bacteria ... | 2009 | 19763409 |
| disruption of the 2-methylcitric acid cycle and evaluation of poly-3-hydroxybutyrate-co-3-hydroxyvalerate biosynthesis suggest alternate catabolic pathways of propionate in burkholderia sacchari. | the objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2mcc) to the catabolism of propionate in burkholderia sacchari. two b. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnm, and the other in acnm and prpc deleted. an operative 2mcc significantly reduces the bacterial ability to incorporate 3-hydroxyvalerate (3hv) into a biodegradable copolyester accumulated from carbohydrates plus propionate. the efficiency of the mut ... | 2009 | 19767840 |
| structural basis of the affinity for oligomannosides and analogs displayed by bc2l-a, a burkholderia cenocepacia soluble lectin. | the opportunistic pathogen burkholderia cenocepacia contains three soluble carbohydrate-binding proteins, related to the fucose-binding lectin pa-iil from pseudomonas aeruginosa. all contain a pa-iil-like domain and two of them have an additional n-terminal domain that displays no sequence similarities with known proteins. printed arrays screening performed on the shortest one, b. cenocepacia lectin a (bc2l-a), demonstrated the strict specificity for oligomannose-type n-glycan structures (lameig ... | 2010 | 19770128 |
| molecular basis of rare aminoglycoside susceptibility and pathogenesis of burkholderia pseudomallei clinical isolates from thailand. | burkholderia pseudomallei is intrinsically resistant to aminoglycosides and macrolides, mostly due to amrab-opra efflux pump expression. we investigated the molecular mechanisms of aminoglycoside susceptibility exhibited by thai strains 708a, 2188a, and 3799a. | 2009 | 19771149 |
| [epidemiological survey of bacteria isolated from the respiratory tract of cystic fibrosis patients]. | with the aim of characterizing the evolution of the epidemiological profile of respiratory bacterial infections of patients having cystic fibrosis (cf), the authors conducted a retrospective analysis about it's incidence and prevalence in 78 cf patients followed at the cf specialized centre, paediatric department, santa maria hospital, lisbon, during a 5 years period (1995-1999). pseudomonas aeruginosa was the most frequently isolated bacteria during the first three years of the study (60-73%), ... | 2003 | 19771691 |
| the pathogenic properties of a novel and conserved gene product, kerv, in proteobacteria. | identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. in this study, we uncover such a factor, termed kerv, in proteobacteria. experiments carried out in a variety of eukaryotic host infection models revealed that the virulence of a pseudomonas aeruginosa kerv null mutant was compromised when it interacted with amoebae, plants, flies, and mice. bioinformatics analyses indicated that kerv is a hypothetical methyltra ... | 2009 | 19779606 |
| [arthritis caused by burkholderia cepacia]. | | 2010 | 19783076 |
| bioactive and structural metabolites of pseudomonas and burkholderia species causal agents of cultivated mushrooms diseases. | pseudomonas tolaasii, p. reactans and burkholderia gladioli pv. agaricicola, are responsible of diseases on some species of cultivated mushrooms. the main bioactive metabolites produced by both pseudomonas strains are the lipodepsipeptides (ldps) tolaasin i and ii and the so called white line inducing principle (wlip), respectively, ldps which have been extensively studied for their role in the disease process and for their biological properties. in particular, their antimicrobial activity and t ... | 2008 | 19787100 |
| proceedings from the 5th world melioidosis congress. khon kaen, thailand. november 21-23, 2007. | | 2008 | 19787859 |
| toll-like receptor signaling in airborne burkholderia thailandensis infection. | melioidosis is a tropical disease endemic in southeast asia and northern australia caused by the gram-negative soil saprophyte burkholderia pseudomallei. although infection is often systemic, the lung is frequently involved. b. thailandensis is a closely related organism that at high doses causes lethal pneumonia in mice. we examined the role of toll-like receptors (tlrs), essential components of innate immunity, in vitro and in vivo during murine b. thailandensis pneumonia. tlr2, tlr4, and tlr5 ... | 2009 | 19797072 |
| differential modulation of burkholderia cenocepacia virulence and energy metabolism by the quorum-sensing signal bdsf and its synthase. | burkholderia cenocepacia produces the molecule cis-2-dodecenoic acid (bdsf), which was previously shown to play a role in antagonism against the fungal pathogen candida albicans by interfering with its morphological transition. in this study, we show that production of bdsf is under stringent transcriptional control and the molecule accumulates in a cell density-dependent manner, typically found with quorum-sensing (qs) signals. b. cenocepacia mutant strain j2315 with a deleted bcam0581 gene, wh ... | 2009 | 19801414 |
| activity of ceftazidime against pseudomonas aeruginosa from bacteraemic and fibrocystic patients. | minimum inhibitory and bactericidal levels of ceftazidime were determined for 337 isolates of pseudomonas aeruginosa (135 mucoid, 202 non-mucoid) derived from fibrocystic patients, and 102 isolates of ps. aeruginosa from blood or csf. fifty per cent of isolates of pq. aeruginosa from all sources were inhibited by 2 mg/l of ceftazidime using inocula of 10(5)-10(6) colony forming units. ninety per cent of the blood-culture and mucoid isolates were inhibited by 4 mg/l of ceftazidime, but more than ... | 1981 | 19802981 |
| in vitro pharmacodynamics of levofloxacin and other aerosolized antibiotics under multiple conditions relevant to chronic pulmonary infection in cystic fibrosis. | the inhalational administration of antibiotics can provide high concentrations locally in the lungs of cystic fibrosis patients and, thus, can be useful for the treatment of chronic bacterial infections. the present study evaluated the in vitro activities of levofloxacin, ciprofloxacin, tobramycin, amikacin, and aztreonam against clinical isolates of pseudomonas aeruginosa, burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and staphylococcus aureus from cystic ... | 2010 | 19805554 |
| innate immune responses of pulmonary epithelial cells to burkholderia pseudomallei infection. | burkholderia pseudomallei, a facultative intracellular pathogen, causes systemic infection in humans with high mortality especially when infection occurs through an infectious aerosol. previous studies indicated that the epithelial cells in the lung are an active participant in host immunity. in this study, we aimed to investigate the innate immune responses of lung epithelial cells against b. pseudomallei. | 2009 | 19806192 |
| bacterial dynamics of biofilm development during toluene degradation by burkholderia vietnamiensis g4 in a gas phase membrane bioreactor. | in this study, the dynamics of living cells (lc) and dead cells (dc) in a laboratory-scale biofilm membrane bioreactor for waste gas treatment was examined. toluene was used as a model pollutant. the bacterial cells were enumerated as fluoromicroscopic counts during a 140 operating day period using baclight nucleic acid staining in combination with epifluorescence and confocal laser scanning microscopy (cslm). overall, five different phases could be distinguished during the biofilm development: ... | 2009 | 19809262 |
| control of lipase enantioselectivity by engineering the substrate binding site and access channel. | lipase from burkholderia cepacia (bcl) has proven to be a very useful biocatalyst for the resolution of 2-substituted racemic acid derivatives, which are important chiral building blocks. our previous work showed that enantioselectivity of the wild-type bcl could be improved by chemical engineering of the substrate's molecular structure. from this earlier study, three amino acids (l17, v266 and l287) were proposed as targets for mutagenesis aimed at tailoring enzyme enantioselectivity. in the pr ... | 2009 | 19816890 |
| burkholderia acidipaludis sp. nov., aluminum-tolerant bacteria isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps in south-east asia. | two strains of aluminium-tolerant bacteria, sa33(t) and 7a078, were isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam (sa33(t)) and thailand (7a078). the strains were gram-negative, aerobic, non-spore-forming rods, 0.6-0.7 mum wide and 1.3-1.7 mum long. these strains showed good growth at ph 3.0-8.0 and 17-37 degrees c. the organisms contained ubiquinone q-8 as the predominant isoprenoid quinone and c(16 ... | 2010 | 19819996 |
| use of a low-dose steroid as an adjunct in the treatment, in mice, of severe sepsis caused by burkholderia pseudomallei. | human melioidosis caused by burkholderia pseudomallei is a severe septic disease that is associated with high mortality, even under appropriate antibiotic treatment. the therapeutic effects of low-dose hydrocortisone plus ceftazidime, and of ceftazidime alone, have recently been investigated in the treatment of acute, severe sepsis caused by b. pseudomallei, both in normal balb/c mice and in balb/c mice with streptozotocin-induced diabetes. the mice were infected and then treated intravenously, ... | 2009 | 19825285 |
| [phenanthren biodegradation and interaction of pseudomonas putida bs3701 and burkholderia sp.bs3702 in plant rhizosphere]. | | 2009 | 19827713 |
| regulation of phenylacetic acid degradation genes of burkholderia cenocepacia k56-2. | metabolically versatile soil bacteria burkholderia cepacia complex (bcc) have emerged as opportunistic pathogens, especially of cystic fibrosis (cf). previously, we initiated the characterization of the phenylacetic acid (pa) degradation pathway in b. cenocepacia, a member of the bcc, and demonstrated the necessity of a functional pa catabolic pathway for full virulence in caenorhabditis elegans. in this study, we aimed to characterize regulatory elements and nutritional requirements that contro ... | 2009 | 19835630 |
| terminal-restriction fragment length polymorphism analysis of biphenyl dioxygenase genes from a polychlorinated biphenyl-polluted soil. | polychlorinated biphenyls (pcbs) are ubiquitous persistent organic pollutants that can be co-metabolically biotransformed by biphenyl-utilizing bacteria. in this study, terminal-restriction fragment length polymorphism (t-rflp) was applied to the substrate specificity-determining region of the 2,3-biphenyl dioxygenase encoding genes of a microbial community found in a pcb-polluted soil. notably, both the total biphenyl/pcb-utilizing community and its members actively expressing the 2,3-biphenyl ... | 2009 | 19835952 |
| pip(2)-binding site in kir channels: definition by multiscale biomolecular simulations. | phosphatidylinositol bisphosphate (pip(2)) is an activator of mammalian inwardly rectifying potassium (kir) channels. multiscale simulations, via a sequential combination of coarse-grained and atomistic molecular dynamics, enabled exploration of the interactions of pip(2) molecules within the inner leaflet of a lipid bilayer membrane with possible binding sites on kir channels. three kir channel structures were investigated: x-ray structures of kirbac1.1 and of a kir3.1-kirbac1.3 chimera and a h ... | 2009 | 19839652 |
| identifying translation initiation sites in prokaryotes using support vector machine. | motivation: gene identification in genomes has been a fundamental and long-standing task in bioinformatics and computational biology. many computational methods have been developed to predict genes in prokaryote genomes by identifying translation initiation site (tis) in transcript data. however, the pseudo-tiss at the genome level make these methods suffer from a high number of false positive predictions. in addition, most of the existing tools use an unsupervised learning framework, whose pred ... | 2010 | 19840808 |
| mixotrophic metabolism in burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of burkholderia kururiensis as burkholderia kururiensis subsp. kururiensis subsp. nov. | a thiosulfate-oxidizing facultative chemolithoautotrophic burkholderia sp. strain atsb13(t) was previously isolated from rhizosphere soil of tobacco plant. strain atsb13(t) was aerobic, gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. strain atsb13(t) exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. a phylogenetic study based on 16s rrna gene sequence analysis indicated that strain atsb13(t) was most closely related to burkholderia ku ... | 2009 | 19841903 |
| lack of correlation of burkholderia pseudomallei quantities in blood, urine, sputum and pus. | we evaluated the correlation of burkholderia pseudomallei quantities in blood versus urine, sputum or pus. correlations between bacterial counts in blood and other samples were not found. it is likely that an initial seeding event to extracellular organs is followed by independent growth of b. pseudomallei, and that bacteria in the urine were not passively filtered from the bloodstream. | 2009 | 19842414 |
| pathema: a clade-specific bioinformatics resource center for pathogen research. | pathema (http://pathema.jcvi.org) is one of the eight bioinformatics resource centers (brcs) funded by the national institute of allergy and infectious disease (niaid) designed to serve as a core resource for the bio-defense and infectious disease research community. pathema strives to support basic research and accelerate scientific progress for understanding, detecting, diagnosing and treating an established set of six target niaid category a-c pathogens: category a priority pathogens; bacillu ... | 2010 | 19843611 |
| the clinical profile and predictors of mortality in patients with melioidosis in south india. | melioidosis is an underdiagnosed and underreported disease in india with protean clinical manifestations. mortality in this study population was 17%. the predominant risk factor for melioidosis was diabetes mellitus. multifocal disease was present in 66% and pulmonary involvement in 61% of patients. in a country like india where the conditions are conducive for endemic melioidosis and due to the clinical similarity of melioidosis to diseases like tuberculosis, it is essential for clinicians to h ... | 2010 | 19850605 |
| purification and crystallization of a putative transcriptional regulator of the benzoate oxidation pathway in burkholderia xenovorans lb400. | burkholderia xenovorans lb400 harbours two paralogous copies of the recently discovered benzoate oxidation (box) pathway. while both copies are functional, the paralogues are differentially regulated and flanked by putative transcriptional regulators from distinct families. the putative lysr-type transcriptional regulator (lttr) adjacent to the megaplasmid-encoded box enzymes, bxe_c0898, has been produced recombinantly in escherichia coli and purified to homogeneity. gel-filtration studies show ... | 2009 | 19851006 |
| biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic burkholderia sp. is-01. | burkholderia sp. is-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3hb-co-3hv)] copolyesters with a high molar fraction of 3hv was isolated from the gut of the adult longicorn beetle, moechotypa diphysis. the strain is-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3hb-co-86.5 mol% 3hv) copolyester when cultivated on a mixture of gluconate (20 g/l) and levulinic acid (12.5 g/l). in this case, the content of t ... | 2009 | 19851740 |
| pahang melioidosis registry. | melioidosis has a high annual incidence and mortality rate in pahang, malaysia. we initiated the first melioidosis registry in the country on 1st july 2005 to improve the management of melioidosis in the state. continuous medical education on melioidosis was carried out in all hospitals in the state to highlight the magnitude of the disease and to educate the doctors on the treatment of the disease. all culture confirmed cases were registered and analysed. during the one-year study period from 1 ... | 2009 | 19852316 |
| differential susceptibility of inbred mouse strains to burkholderia thailandensis aerosol infection. | burkholderia pseudomallei is the causative agent of melioidosis, an emerging bacterial disease that accounts for high rates of septicaemia and death in parts of southeast asia and northern australia. the closely related species burkholderia thailandensis is considered avirulent in humans and has been used as a surrogate for b. pseudomallei in several studies. the pathogenesis of b. pseudomallei and the role of toll-like receptors (tlrs) in host immunity to infection are not well-defined. in this ... | 2010 | 19853031 |
| icmf is a fusion between the radical b12 enzyme isobutyryl-coa mutase and its g-protein chaperone. | coenzyme b(12) is used by two highly similar radical enzymes, which catalyze carbon skeleton rearrangements, methylmalonyl-coa mutase and isobutyryl-coa mutase (icm). icm catalyzes the reversible interconversion of isobutyryl-coa and n-butyryl-coa and exists as a heterotetramer. in this study, we have identified >70 bacterial proteins, which represent fusions between the subunits of icm and a p-loop gtpase and are currently misannotated as methylmalonyl-coa mutases. we designate this fusion prot ... | 2010 | 19864421 |
| [doripenem: need for a new carbapenem]. | doripenem is a new member of the carbapenem family. its spectrum is a little wider than meropenem and it is active on some pseudomonas aeruginosa strains resistant to other carbapenems and on burkholderia cepacia. doripenem was evaluated in nosocomial pneumonia including ventilator-acquired pneumonia, complicated intra-abdominal infection, and complicated urinary tract infection. in nosocomial pneumonia, doripenem showed an interesting activity in p. aeruginosa infected patients. this data, alon ... | 2009 | 19875257 |
| the active site of an enzyme can host both enantiomers of a racemic ligand simultaneously. | | 2009 | 19876985 |
| topological analysis of a haloacid permease of a burkholderia sp. bacterium with a phoa-lacz reporter. | 2-haloacids can be found in the natural environment as degradative products of natural and synthetic halogenated compounds. they can also be generated by disinfection of water and have been shown to be mutagenic and to inhibit glyceraldehyde-3-phosphate dehydrogenase activity. we have recently identified a novel haloacid permease deh4p from a bromoacetate-degrading bacterium burkholderia sp. mba4. comparative analyses suggested that deh4p is a member of the major facilitator superfamily (mfs), w ... | 2009 | 19878597 |
| the lipid a of burkholderia multivorans c1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model. | cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. burkholderia multivorans and burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. lipopolysaccharide endotoxins (lpss) are among the foremost factors of pathogenesis of gram-negative infection and, in particular, lipid a is the endotoxic portion of lps r ... | 2010 | 19880661 |
| burkholderia mallei cluster 1 type vi secretion mutants exhibit growth and actin polymerization defects in raw 264.7 murine macrophages. | burkholderia mallei is a facultative intracellular pathogen that causes severe disease in animals and humans. recent studies have shown that the cluster 1 type vi secretion system (t6ss-1) expressed by this organism is essential for survival in a hamster model of glanders. to better understand the role of t6ss-1 in the pathogenesis of disease, studies were initiated to examine the interactions of b. mallei tsse mutants with raw 264.7 murine macrophages. results obtained by utilizing modified gen ... | 2010 | 19884331 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| effects of nutrients on quorum signals and secondary metabolite productions of burkholderia sp. o33. | several bioactive metabolites, including pyrrolnitrin, nacylhomoserine lactones, and polyhydroxyalkanoates were isolated from burkholderia sp. o33. effects of various nutrients, including sugars, gluconolactone, glycerol, tryptophan, chloride, and zinc were investigated in relation to the production of these metabolites. logarithmic increase of pyrrolnitrin was observed between 2-5 days and reached a maximum at 7-10 days. tryptophan concentration reached the maximum at 3 days, whereas 7-chlorotr ... | 2009 | 19884772 |
| computational study of the lipase-mediated desymmetrisation of 2-substituted-propane-1,3-diamines. | the enantioselectivity displayed by the lipase from pseudomonas cepacia towards a wide range of prochiral 2-substituted-propane-1,3-diamines was studied by means of molecular dynamics simulations (mds). in all cases the enzyme allows the recovery of the corresponding amino carbamates of r configuration. however, the enantioselectivity is only synthetically useful if no ortho substituent is present and the aromatic ring is directly bonded to the 2-carbon of the 1,3-diamine core. analysis of the m ... | 2009 | 19885896 |
| backbone and side chain (1)h, (13)c, and (15)n nmr assignments for the organic hydroperoxide resistance protein (ohr) from burkholderia pseudomallei. | burkholderia pseudomallei is a niaid category b microorganism responsible for melioidosis. here we report backbone and side chain nmr assignments for the 139-residue, homodimeric, organic hydroperoxide resistance protein (ohr) from this organism. | 2009 | 19888681 |
| oxidative stress of burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. | burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (cf). a cf population in the czech republic experienced an epidemic outbreak caused by a b. cenocepacia st-32 strain. the clonality of the isolates was evident by multilocus sequence typing; however, fingerprinting profiles obtained by pulsed-field gel electrophoresis (pfge) showed substantial band variability. we investigated whether the pfge pattern diversity resulted from genomic rearrangement ... | 2010 | 19889907 |
| burkholderia pseudomallei misidentified by automated system. | after returning from thailand, a 35-year-old man from switzerland was hospitalized with an abscess of the head. material cultured from the abscess and adjacent bone grew a gram-negative rod, which was misidentified by an automated microbiology system as burkholderia cepacia. the organism was eventually identified by molecular methods as b. pseudomallei. | 2009 | 19891868 |
| imported melioidosis, israel, 2008. | in 2008, melioidosis was diagnosed in an agricultural worker from thailand in the southern jordan valley in israel. he had newly diagnosed diabetes mellitus, fever, multiple abscesses, and osteomyelitis. burkholderia pseudomallei was isolated from urine and blood. four of 10 laboratory staff members exposed to the organism received chemoprophylaxis, 3 of whom had adverse events. | 2009 | 19891871 |
| [a sensitive, specific and predictive isolation medium developed for stenotrophomonas maltophilia study in healthcare settings]. | stenotrophomonas maltophilia (smalto) is a prominent nosocomial pathogen, commonly isolated in the hospital environment. multiple smalto nosocomial outbreaks have been linked to contaminated water sources. this study aimed to develop a medium able to ease healthcare environment smalto isolation. | 2010 | 19892479 |
| mycotic iliac aneurysm associated with burkholderia pseudomallei. | | 2010 | 19897393 |
| cloning and overexpression of the xylose isomerase gene from burkholderia sacchari and production of polyhydroxybutyrate from xylose. | a different organization for the xyl operon was found in different genomes of burkholderia and pseudomomas species. degenerated primers were designed based on burkholderia genomes and used to amplify the xylose isomerase gene (xyla) from burkholderia sacchari ipt101. the gene encoded a protein of 329 amino acids, which showed the highest similarity (90%) to the homologous gene of burkholderia dolosa. it was cloned in the broad host range plasmid pbbr1mcs-2, which partially restored growth and po ... | 2009 | 19898542 |
| false-positive widal in melioidosis. | enteric fever is endemic in this part of the world, and widal test is one of the time-honored laboratory tests that are being used for years to diagnose the disease. on the other hand, melioidosis is a newly emerging disease from this region, which is most often misdiagnosed or underdiagnosed by clinicians. it is well accepted that false-positive widal reactions following certain non-typhoid salmonella infections may occur commonly. three cases of high titers of widal test are described, where m ... | 2009 | 19901486 |
| genomic transcriptional profiling identifies a candidate blood biomarker signature for the diagnosis of septicemic melioidosis. | melioidosis is a severe infectious disease caused by burkholderia pseudomallei, a gram-negative bacillus classified by the national institute of allergy and infectious diseases (niaid) as a category b priority agent. septicemia is the most common presentation of the disease with a 40% mortality rate even with appropriate treatments. better diagnostic tests are therefore needed to improve therapeutic efficacy and survival rates. | 2009 | 19903332 |
| colloidal and bacterial deposition: role of gravity. | the role of gravitational force on the deposition of 0.5, 1.1, and 1.8 mum carboxylate-modified polystyrene latex (cml) microspheres and bacterium burkholderia cepacia g4g has been evaluated using a parallel plate flow chamber system. this experimental system utilized an inverted and an upright optical microscope attached with image-capturing devices to directly observe and determine the deposition kinetics onto glass surfaces located at the top and bottom of the flow chamber. deposition kinetic ... | 2010 | 19911823 |