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vector competence of five common mosquito species in the people's republic of china for western equine encephalitis virus.two strains of the western equine encephalitis virus (weev) were first detected and isolated in china in 2001. the maintenance and transmission cycles of weev in china are currently not well understood, and the mosquito vectors involved in these cycles are unknown. to understand the ability of the local mosquitoes in china to transmit weev, the vector competence of five mosquito species, namely, culex pipiens pallens coquillett, cx. p. quinquefasciatus say, aedes (stegomyia) albopictus skuse, ae ...201222276651
[coevolution of symbiotic bacteria wolbachia and host mtdna in russian populations of the culex pipiens mosquito complex].the mitochondrial dna phylogenies of closely related forms of mosquitoes from the culex pipiens complex and of strains of the endosymbiotic bacteria wolbachia pipientis were compared. based on the cytochrome oxidase subunit i gene polymorphism, six mitochondrial haplotypes and four w. pipientis groups were discovered in mosquitoes from geographically remote populations. a strict correlation between the coi type and the type of w. pipientis proves the stable coinheritance and distribution of both ...201425739293
male mating performance and cytoplasmic incompatibility in a wpip wolbachia trans-infected line of aedes albopictus (stegomyia albopicta).wolbachia pipientis hertig (rickettsiales: rickettsiaceae) is a maternally inherited endosymbiont of a large number of insects and other arthropods that induces various effects on host reproductive biology. among these, cytoplasmic incompatibility (ci) is a form of sterility induced in eggs produced by mating between infected males and females uninfected or infected by an incompatible wolbachia strain. this phenomenon has been proposed as a potential way to produce functionally sterile males to ...201323171418
molecular xenomonitoring (mx) and transmission assessment survey (tas) of lymphatic filariasis elimination in two villages, menoufyia governorate, egypt.lymphatic filariasis (lf) is focally endemic in egypt where the female mosquito, culex pipiens, is responsible for its transmission. the aim of the study was to investigate the impact of implementation of the 13th round of mda in two egyptian villages in the menoufyia governorate area after failing the transmission assessment survey (tas) in 2005 using two methods, and to decide whether it is safe to stop mda in these, as well as in similar implementation units (ius). to achieve this aim, both t ...201728155014
mosquito identification and molecular xenomonitoring of lymphatic filariasis in selected endemic areas in giza and qualioubiya governorates, egypt.lymphatic filariasis is a vector-borne health problem that has been focally endemic in egypt for centuries. the chief vectors of transmission are culicinae species. control measures in the form of mass drug administration of dec citrate treatment have been implemented in nile delta for almost a decade. this study aimed to identify the prevalent mosquito species in endemic areas in giza and qualioubiya governorates and to monitor wuchereria bancrofti infection by detecting the parasite dna in col ...201627363044
effects of midgut bacteria and two protease inhibitors on the transmission of wuchereria bancrofti by the mosquito vector, culex pipiens.laboratory investigations were carried out to study the effect of two protease inhibitors on the transmission of w. bancrofti filarial by culex pipiens and to study the susceptibility interaction between filaria and protease inhibitors. the results obtained revealed that, infection rates were variable among untreated and treated symbiotic and aposymbiotic cx. pipiens females resulted from third instar larvae treated with e-64 and edta. the survival rate was variable among untreated and treated s ...201324260833
effects of midgut bacteria and two protease inhibitors on the reproductive potential and midgut enzymes of culex pipiens infected with wuchereria bancrofti.laboratory investigations were carried out to asses the effect of some proteases inhibitors on the reproductive potential of culex pipiens females resulted from larvae treated with different protease inhibitors. the fecundity and engorgement of symbiotic and aposymbiotic c. pipiens females were significantly reduced. the blood meal digestion period increased significantly. on the other hand, enzyme band with molecular weight of 40 kda which may be cysteine protease was detected in untreated symb ...201324260832
population ecology of mosquitoes and the status of bancroftian filariasis in el dakahlia governorate, the nile delta, egypt.mosquitoes were surveyed (oct. 2010 & apr. - oct. 2011) in some localities representing 13 centers of el-dakahlia governorate. six mosquito species were collected: culex pipiens, cx. antennatus, cx. perexiguus, ochlerotatus detritus, anopheles pharoensis and an. tenebrosus. culex pipiens was predominating (ca 79% larvae, 51% adults). culex antennatus and cx. perexiguus were also common. of the four types of the breeding habitats, the drainage canals were the most productive (53.4% larvae). for t ...201323697019
experimental transmission of zika virus by mosquitoes from central europe.mosquitoes collected in germany in 2016, including culex pipiens pipiens biotype pipiens, culex torrentium and aedes albopictus, as well as culex pipiens pipiens biotype molestus (in colony since 2011) were experimentally infected with zika virus (zikv) at 18 °c or 27 °c. none of the culex taxa showed vector competence for zikv. in contrast, aedes albopictus were susceptible for zikv but only at 27 °c, with transmission rates similar to an aedes aegypti laboratory colony tested in parallel.201728106528
culex species mosquitoes and zika virus.recent reports of zika virus (zikv) isolates from culex species mosquitoes have resulted in concern regarding a lack of knowledge on the number of competent vector species for zikv transmission in the new world. although observations in the field have demonstrated that zikv isolation can be made from culex species mosquitoes, the detection of zikv in these mosquitoes is not proof of their involvement in a zikv transmission cycle. detection may be due to recent feeding on a viremic vertebrate, an ...201627556838
mosquito c-type lectins maintain gut microbiome homeostasis.the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ...201627572642
phylogenetic analysis of the atp-binding cassette transporter family in three mosquito species.the atp-binding cassette (abc) transporter family functions in the atp-dependent transportation of various substrates across biological membranes. abc proteins participate in various biological processes and insecticide resistance in insects, and are divided into eight subfamilies (a-h). mosquitoes are important vectors of human diseases, but the mechanism by which the abc transporter family evolves in mosquitoes is unknown. in this study, we classified and compared the abc transporter families ...201627521922
plasmodium delichoni n. sp.: description, molecular characterisation and remarks on the exoerythrocytic merogony, persistence, vectors and transmission.malaria parasite plasmodium (novyella) delichoni n. sp. (haemosporida, plasmodiidae) was found in a widespread eurasian songbird, the common house martin delichon urbicum (hirundinidae). it is described based on the morphology of its blood stages and segments of the mitochondrial cytochrome b and apicoplast genes, which can be used for molecular identification of this species. erythrocytic meronts and gametocytes are strictly nucleophilic, and mature gametocytes possess pigment granules of marke ...201627000087
bioactivity and laundering resistance of five commercially available, factory-treated permethrin-impregnated fabrics for the prevention of mosquito-borne diseases: the need for a standardized testing and licensing procedure.personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. in this regard, a major advance has been the development of residual insecticides that can be impregnated into clothing. currently, however, information on specific treatment procedures, initial insecticide concentrations, arthropod toxicity, residual activity, and laundering resistance is either fragmentary or non-existent, and no world health organization pesticides ...201626738734
modification of the suna trap for improved survival and quality of mosquitoes in support of epidemiological studies.monitoring adult mosquito populations provides information that is critical for assessing risk of vector-borne disease transmission. the recently developed suna trap was found to be a very effective trap when baited with an attractive odor blend. a modification of this trap was tested to improve its function as a tool for monitoring mosquito populations, including anopheles coluzzii (an. gambiae sensu stricto molecular form m), aedes aegypti, and culex pipiens. the modified suna trap (suna-m) wa ...201526375903
larvicidal activity of naturally occurring naphthalenedione and its structurally related analogs against three mosquito species.the objective of this study was to determine the larvicidal activity of an active compound isolated from cercis chinensis heartwood and its structurally related analogs against 4th-stage aedes aegypti, culex pipiens pallens, and ae. togoi. the larvicidal compound of c. chinensis was isolated with the use of various chromatographic techniques and identified as analogs of 1,4-naphthalenedione. based on the median lethal concentration (lc(50)) values of commercially procured analogs against ae. aeg ...201525843178
nectar protein content and attractiveness to aedes aegypti and culex pipiens in plants with nectar/insect associations.we chose five easily propagated garden plants previously shown to be attractive to mosquitoes, ants or other insects and tested them for attractiveness to culex pipiens and aedes aegypti. long term imbibition was tested by survival on each plant species. both mosquito species survived best on impatiens walleriana, the common garden impatiens, followed by asclepias curassavica, campsis radicans and passiflora edulis, which sponsored survival as well as the 10% sucrose control. immediate preferenc ...201525792420
detection and isolation of the α-proteobacterium asaia in culex mosquitoes.investigations of microbiota within mosquitoes continue to widen the spectrum of possible symbiont-based applications against vector-borne diseases. in this context, α-proteobacteria of the genus asaia (rhodospirillales: acetobacteraceae) are emerging as possible endosymbiotic candidates, particularly in paratransgenic approaches aimed at interrupting pathogen transmission. previous studies have shown that asaia spp. distribution among anopheles gambiae and anopheles stephensi (diptera: culicida ...201425387864
larvicidal activity of the active constituent isolated from tabebuia avellanedae bark and structurally related derivatives against three mosquito species.mosquito larvicidal activities of active constituent isolated from tabebuia avellanedae bark and its structurally related derivatives were examined against the fourth instar larvae of aedes aegypti, culex pipiens pallens, and ochlerotatus togoi. the insecticidal constituent of t. avellanedae bark was isolated by chromatographic techniques and identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthalenedione. on the basis of the 50% lethal concentration (lc50) values against c. pipiens pallens ...201324144401
ovicidal activity of three insect growth regulators against aedes and culex mosquitoes.interspecific variations in the susceptibility of freshly and embryonated eggs of aedes albopictus, ae. aegypti, ae. atropalpus and culex pipiens were tested against three classes of insect growth regulators (igrs) including ecdysone agonist (azadirachtin), chitin synthesis inhibitor (diflubenzuron) and juvenile hormone analog (pyriproxyfen) at 0.001, 0.01, 0.1 and 1.0ppm concentrations. egg hatching inhibition was dose dependent, the highest being at 1.0ppm concentration for freshly laid eggs o ...201323860181
[characterization of bacillus thuringrensis var. israelesis larvicide granules to larvae of mosquitoes].to develop a new formulation of bacillus thuringrensis var. israelesis (bti) granules and assess its effect on mosquito control.201323687813
larvicidal activity of cnidium monnieri fruit coumarins and structurally related compounds against insecticide-susceptible and insecticide-resistant culex pipiens pallens and aedes aegypti.an assessment was made of the toxicity of imperatorin and osthole identified in cnidium monnieri fruit, 11 related compounds and five insecticides to larvae from insecticide-susceptible culex pipiens pallens (ks-cp strain) and aedes aegypti and wild c.p. pallens (ys-cp colony) using a direct-contact mortality bioassay. results were compared with those of the conventional larvicide temephos.201222389164
a 2-yr mosquito survey focusing on aedes koreicus (diptera: culicidae) in northern italy and implications for adult trapping.aedes koreicus (edwards) is an invasive mosquito species, like aedes albopictus (skuse) and aedes japonicus japonicus (theobald), that has already colonized a large part of northeastern italy and other european countries. despite its rapid expansion, information about adult distribution and trapping is lacking. here, we conducted a 2-yr longitudinal survey using adult traps to investigate the spatiotemporal distribution of ae. koreicus and evaluated the effectiveness of three trapping devices in ...201728399310
baseline insecticide susceptibility screening against six active ingredients for culex and aedes (diptera: culicidae) mosquitoes in the united states.mosquitoes may develop resistance to insecticide active ingredients (ai). thus, mosquitoes should be tested for resistance to confirm efficacy of insecticide-based control, inform management decisions, and protect public and environmental health. our objectives were to determine a baseline of resistance for six ais used in mosquito control in the united states to assess how resistance differs between mosquito collection location, ai, and mosquito species (container-ovipositing aedes and culex th ...201728399272
the effect of interspecific competition on the temporal dynamics of aedes albopictus and culex pipiens.aedes albopictus and culex pipiens larvae reared in the same breeding site compete for resources, with an asymmetrical outcome that disadvantages only the latter species. the impact of these interactions on the overall ecology of these two species has not yet been assessed in the natural environment. in the present study, the temporal patterns of adult female mosquitoes from both species were analysed in north-eastern italy, and substantial temporal shifts between abundance curves of cx. pipiens ...201728228159
identification of larvicidal constituents of the  essential oil of echinops grijsii roots against the  three species of mosquitoes.the screening of chinese medicinal herbs for insecticidal principles showed that the essential oil of echinops grijsii hance roots possessed significant larvicidal activity against mosquitoes. the essential oil was extracted via hydrodistillation and its constituents were determined by gas chromatography-mass spectrometry (gc-ms) analysis. gc-ms analyses revealed the presence of 31 components, with 5-(3-buten-1-yn-1-yl)-2,2'-bithiophene (5-bbt, 27.63%), αterthienyl (α-t, 14.95%),1,8-cineole (5.5 ...201728134799
malaria transmission and the sensitivity of aggressive mosquitoes to insecticides in a poorly urbanized area of the deido health district in douala (cameroon).the design of an appropriate strategy for mosquito control in urban areas must take into account the local ecoclimate and its particularities. this study sought to determine the dynamics of malaria transmission and the level of insecticide susceptibility of aggressive mosquitoes in ndogbong, a poorly urbanized district of douala. mosquitoes were captured on volunteers in 4 houses and outdoors for 2 consecutive nights a month, from april to september 2014, and identified by molecular and morpholo ...201728132950
west virginia mosquitoes: sequential list by publication, newly found species, corrections, and notes for earlier records.a chronological list of species based on date of publication is provided for confirmed mosquito species in west virginia. five additional newly found species in the state are documented with collection data. two previously misidentified or mislabeled published records are corrected. the number of confirmed species occurring in the state is now 35, and many more species are likely present, but undetected. significant populations of 4 widely distributed known vector species, aedes albopictus , ae. ...201627802407
abundance and distribution of immature mosquitoes in urban rivers proximate to their larval habitats.whether ecological restoration of polluted urban rivers would provide suitable breeding habitats for some mosquitoes was not clear yet. it was therefore important to determine how altered river conditions influence mosquito ecology. monthly data on water quality and larval density were obtained to determine the effects of river systems on the distribution and abundance of immature mosquitoes in two coastal cities in eastern china. in total, 5 species within two genera of mosquitoes were collecte ...201627515809
molecular detection of six (endo-) symbiotic bacteria in belgian mosquitoes: first step towards the selection of appropriate paratransgenesis candidates.actually, the use of symbiotic bacteria is one of alternative solution to avoid vector resistance to pesticides. in belgium, among 31 identified mosquito species, 10 were considered as potential vectors. given to introduction risks of arbovirosis, the purpose of this study was to investigate the presence of symbiosis bacteria in potential mosquito vectors. eleven species caught from 12 sites in belgium were used: culex pipiens s.l., culex torrentium, culex hortensis, anopheles claviger, anophele ...201626670313
species composition and seasonal distribution of mosquito larvae (diptera: culicidae) in southern new jersey, burlington county.a total of 36,495 larvae consisting of 45 species from 11 genera were collected from 7,189 sites from southern new jersey, burlington county between the months of march and october, 2001-2014. density and seasonal distribution were determined among natural and artificial habitat. the most dominant species collected from natural habitat was aedes vexans (meigen) followed by ochlerotatus canadensis canadensis (theobald), culex restuans theobald, culex pipiens l., and culex territans walker. the mo ...201526336214
macrocyclops albidus (copepoda: cyclopidae) for the biocontrol of aedes albopictus and culex pipiens in italy.the aim of our study was to assess the potential of macrocyclops albidus as a biological control agent against the 1st and 2nd instars of culex pipiens and aedes albopictus. under laboratory conditions of prey saturation (50 1st instars/copepod), an average of 58.98% of cx. pipiens and 54.99% of ae. albopictus larvae were killed by 1 copepod in 24 h. trials run in big drums containing 200 liters of water showed that the m. albidus population, inoculated in april, efficiently controlled the mosqu ...201525843174
susceptibility of field-collected mosquitoes in central new jersey to organophosphates and a pyrethroid.chemical insecticides are the primary means to control mosquitoes, and mosquito control programs must regularly monitor for resistance of mosquito vectors to commonly used insecticides to ensure the efficacy and sustainability of active ingredients. we performed insecticide resistance bioassays to test the susceptibility of field-collected mosquitoes in central new jersey to 1 larvicide (temephos) and 2 adulticides (malathion and sumithrin). larval susceptibility of culex pipiens pipiens to teme ...201425102600
a multiyear surveillance for aedes albopictus with biogents sentinel trap counts for males and species composition of other mosquito species.the biogents sentinel (bgs) trap is a very effective tool to monitor adult populations of aedes albopictus. we utilized bgs traps during an intensive 5-year surveillance as part of an "area-wide management program for the asian tiger mosquito." during this period, >52,000 mosquitoes were collected, comprising a total of 24 species. the most abundant species was ae. albopictus (54.4%) followed by culex spp. (8.7%), which primarily comprised culex pipiens pipiens (6.5%) and cx. restuans (0.9%). we ...201425102596
distribution and mosquito hosts of chaoyang virus, a newly reported flavivirus from the republic of korea, 2008-2011.in total, 183,602 female culicine mosquitoes were captured by mosquito magnet, black light, and new jersey light traps, and manual aspiration of resting blood-fed mosquitoes, in the republic of korea from 2008 to 2011. culicine mosquitoes were identified to species, placed in pools of up to 30 mosquitoes each, and screened for flavivirus rna by using an sybr green i-based reverse transcription-polymerase chain reaction assay. thirty-two of the 8,199 pools assayed were positive by quantitative po ...201424724298
socioeconomic status affects mosquito (diptera: culicidae) larval habitat type availability and infestation level.mosquito populations are largely regulated by processes occurring at the larval stage. we sampled mosquito larval microhabitats (mostly water-holding containers) in six neighborhoods in the washington, dc, area that varied in socioeconomic status (ses) and housing structure (row houses vs. stand-alone houses) to test associations among these neighborhood characteristics, microhabitat abundance and parameters, and mosquito occurrence and densities. thirty-four percent (33.9%) of sampled microhabi ...201323926774
successional mosquito dynamics in surrogate treehole and ground-container habitats in the northeastern united states: where does aedes albopictus fit in?this study assessed the risk of larval displacement of the eastern treehole mosquito, aedes triseriatus, and the northern house mosquito, culex pipiens, by aedes albopictus, the asian tiger mosquito, during the establishment and successional stages of novel larval mosquito treehole and ground-container habitats in the state of new jersey, u.s.a. culex pipiens and culex restuans were the first mosquito species to colonize ground-container habitats and were the dominant larval species throughout t ...201323701622
linking mosquito infestation to resident socioeconomic status, knowledge, and source reduction practices in suburban washington, dc.eliminating water-holding containers where mosquitoes oviposit and develop (source reduction) can help manage urban disease-vector mosquitoes. source reduction requires residents to be knowledgeable of effective practices and motivated to implement them. we tested relationships between demographics, resident knowledge, attitudes, and practices (kap), and mosquito infestation by administering larval mosquito surveys and kap questionnaires in washington, dc. respondents who reported practicing sou ...201323377982
evaluation of commercial products for personal protection against mosquitoes.human landing catch studies were conducted in a semi-field setting to determine the efficacy of seven commercial products used for personal protection against mosquitoes. experiments were conducted in two empty, insecticide free, mesh-enclosed greenhouses, in israel, with either 1500 aedes albopictus or 1500 culex pipiens released on consecutive study nights. the products tested in this study were the off!(®) clip-on™ mosquito repellent (metofluthrin 31.2%) and the terminix(®) allclear(®) sideki ...201323092689
reduction of mosquito biting pressure by timed-release 0.3% aerosolized geraniol.we conducted a study to determine the degree of personal protection provided by the terminix(®) allclear(®) mosquito mister - lantern edition. this outdoor unit was operated to disperse an aerosolized aqueous 0.3% geraniol emulsion in timed-release intervals of 5.0, 7.5, and 10.0 min. human volunteers participated in landing catch experiments to test the effect of geraniol sprayed at pre-set time intervals, at two distances: (1) 18 ft (5.49 m), the maximum effective distance claimed by the manuf ...201222750481
comparative morphology of the pyloric armature of adult mosquitoes (diptera: culicidae).the structure of the pyloric armature, hypothesized to aid in blood-meal digestion or parasite resistance, was compared quantitatively among the following 8 species in 5 genera of adult mosquitoes from the southeastern united states: aedes albopictus, aedes japonicus, aedes triseriatus, anopheles punctipennis, culex pipiens s.l., culex restuans, orthopodomyia signifera, and toxorhynchites rutilus. females differed significantly among species in the structure of spines composing the armature, wit ...201222548826
tree-hole breeding mosquitoes in israel.a survey was conducted to evaluate the number of tree-hole breeding mosquito species and their distribution in the six principal woodland types in israel. out of approximately 3,000 mature trees examined, only 38 contained holes that retained water for extended periods of time, and breeding mosquitoes were observed in 27 of them. two specialized tree-hole breeders, aedes pulchritarsis rondani and aedes geniculatus oliver, were found breeding at several sites in northern israel, always at locatio ...201222548543
blood-feeding ecology of mosquitoes in zoos.to determine if the unique host assemblages in zoos influence blood-feeding by mosquitoes (diptera: culicidae), a sampling programme was conducted in greenville and riverbanks zoos, south carolina, u.s.a., from april 2009 to october 2010. a total of 4355 female mosquitoes of 14 species were collected, of which 106 individuals of nine species were blood-fed. the most common taxa were aedes albopictus (skuse), aedes triseriatus (say), anopheles punctipennis (say), culex erraticus (dyar & knab), cu ...201222390304
weak larval competition between the invasive mosquito aedes japonicus japonicus (diptera: culicidae) and three resident container-inhabiting mosquitoes in the laboratory.the spread of exotic mosquito species into new environments can introduce shifts in mosquito populations and potentially alter public health risks to mosquito-borne diseases. the successful establishment of exotic species may occur due to their competitive advantage over other cohabitating species. we hypothesized that the recently introduced exotic mosquito aedes japonicus japonicus (theobald) would be a more effective competitor than aedes atropalpus (coquillett) and aedes triseriatus (say), a ...201222493844
hyperparasitism of mosquitoes by water mite larvae.hyperparasitism of ectoparasitic water mite larvae on mosquitoes is still a neglected relationship and was investigated only in a few studies. we analysed 2313 female mosquitoes from six different sampling localities with regard to their degree of parasitism with water mite larvae. in total, we found 38 mosquito individuals parasitized by 93 water mite larvae, ranging from 1 to 12 larvae per mosquito. water mite larvae detected are members of the two species parathyas cf. barbigera (n = 92) and ...201525899329
evaluation of the repellent effects of nepeta parnassica extract, essential oil, and its major nepetalactone metabolite against mosquitoes.the dichloromethane-methanol extract, the essential oil obtained by hydrodistillation from nepeta parnassica, and the isolated 4aα,7α,7aβ-nepetalactone were evaluated for their repellent effect against the mosquitoes aedes (stegomyia) cretinus edwards and culex pipiens pipiens biotype molestus forskål. the chemical analysis of n. parnassica essential oil, dominated by oxygenated monoterpenes (87 %), revealed 4aα,7α,7aβ-nepetalactone (36.8%), 1,8-cineole (25.5%), and 4aα,7β,7aβ-nepetalactone (11. ...201424449446
comparison of adult mosquito black-light and light-emitting diode traps at three cowsheds located in malaria-endemic areas of the republic of korea.adult mosquito surveillance and field trials evaluated selected commercially available ultraviolet black-light (bl) and light-emitting diode (led) traps at three sites where vivax malaria is endemic from may to october 2015 in northwestern republic of korea. collections totaled 283,929 adult mosquitoes (280,355 [98.74%] females and 3,574 [1.26%] males) comprising 17 species (including six members of the anopheles hyrcanus group) belonging to six genera. the four most predominant female species c ...201728082650
comparison of adult mosquito black-light and light-emitting diode traps at three cowsheds located in malaria-endemic areas of the republic of korea.adult mosquito surveillance and field trials evaluated selected commercially available ultraviolet black-light (bl) and light-emitting diode (led) traps at three sites where vivax malaria is endemic from may to october 2015 in northwestern republic of korea. collections totaled 283,929 adult mosquitoes (280,355 [98.74%] females and 3,574 [1.26%] males) comprising 17 species (including six members of the anopheles hyrcanus group) belonging to six genera. the four most predominant female species c ...201627638960
seasonal and daily activity patterns of mosquito (diptera: culicidae) vectors of pathogens in northeastern italy.the seasonal and daily activity of mosquito vectors of pathogens affecting animals and humans were studied in northeastern italy at a site within the po river delta park. a cdc-co2 trap and a gravid trap were operated at 2-h intervals for 24 h every 15 d from may to october 2010. overall, 5,788 mosquitoes comprising six species were collected, namely culex pipiens l. (75.1% of total), aedes caspius (pallas) (15.2%), aedes vexans (meigen) (6.9%), anopheles maculipennis s.l. meigen (2.6%), culiset ...201526336280
methods of mosquito plague control in świnoujście area based on the analysis of species distribution.studies conducted in 2013 in świnoujście area aimed at determining mosquito species distribution and analyzing sites, productivity and dynamics of larva and adult breeding as well as developing guidelines for effective mosquito control in this area.201525862454
description of the first cryptic avian malaria parasite, plasmodium homocircumflexum n. sp., with experimental data on its virulence and development in avian hosts and mosquitoes.for over 100 years studies on avian haemosporidian parasite species have relied on similarities in their morphology to establish a species concept. some exceptional cases have also included information about the life cycle and sporogonic development. more than 50 avian plasmodium spp. have now been described. however, pcr-based studies show a much broader diversity of haemosporidian parasites, indicating the possible existence of a diverse group of cryptic species. in the present study, using bo ...201525449950
stage and age structured aedes vexans and culex pipiens (diptera: culicidae) climate-dependent matrix population model.aedes vexans and culex pipiens mosquitoes are potential vectors of many arbovirial diseases. due to the ongoing climate changes and reappearance of some zoonoses that were considered eradicated, there is a growing concern about potential disease outbreaks. therefore, the prediction of increased adult population abundances becomes an essential tool for the appropriate implementation of mosquito control strategies. in order to describe the population dynamics of a. vexans and c. pipiens mosquitoes ...201322971424
cross-correlation map analyses show weather variation influences on mosquito abundance patterns in saginaw county, michigan, 1989-2005.weather is important determinant of mosquito abundance that, in turn, influences vectorborne disease dynamics. in temperate regions, transmission generally is seasonal as mosquito abundance and behavior varies with temperature, precipitation, and other meteorological factors. we investigated how such factors affected species-specific mosquito abundance patterns in saginaw county, mi, during a 17-yr period. systematic sampling was undertaken at 22 trapping sites from may to september, during 1989 ...201222897045
toxicity of amorphigenin from the seeds of amorpha fruticosa against the larvae of culex pipiens pallens (diptera: culicidae).the larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, culex pipiens pallens after 24 h exposure. of the tested extracts, the ethanol one exhibited the highest larvicidal activity (lc50 = 22.69 mg/l). amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with lc50 and lc90 values ...201525690287
mosquitoes (diptera: culicidae) of metropolitan hamburg, germany.in europe, mosquito-related public health concerns are growing due to the increasing spread of invasive mosquito species and the recent emergence of mosquito-borne arboviruses. a vital backbone in the assessment of these issues is detailed knowledge of the mosquito fauna, i.e. regional mosquito inventories. it was therefore decided to intensify nationwide investigations on the occurrence and distribution of mosquitoes in germany in order to update old records and to detect possible faunal change ...201424870250
blood-feeding patterns of native mosquitoes and insights into their potential role as pathogen vectors in the thames estuary region of the united kingdom.the range of vertebrate hosts on which species of mosquito blood-feed is an important parameter for identifying potential vectors and in assessing the risk of incursion and establishment of vector-borne pathogens. in the united kingdom, studies of mosquito host range have collected relatively few specimens and used techniques that could only broadly identify host species. this study conducted intensive collection and analysis of mosquitoes from a grazing marsh environment in southeast england. t ...201728347323
species composition, co-occurrence, association and affinity indices of mosquito larvae (diptera: culicidae) in mazandaran province, northern iran.although considerable progress has been made in the past years in management of mosquito borne diseases such as malaria, dengue, yellow fever and west nile fever through research in biology and ecology of the vectors, these diseases are still major threats to human health. therefore, more research is required for better management of the diseases. this investigation provides information on the composition, co-occurrence, association and affinity indices of mosquito larvae in mazandaran province, ...201626805471
a novel window entry/exit trap for the study of endophilic behavior of mosquitoes.to overcome the limitations of currently used window entry/exit traps, we developed an efficient new glue net entry/exit trap (gnt) that is economical, easily transported and assembled, and can be utilized for a variety of studies which aim to investigate the dynamics of mosquito movements between indoor and outdoor habitats. cage experiments were conducted to determine what percentage of mosquitoes trying to pass through the netting are actually being caught. the gnt caught 97% of female and 98 ...201728027886
identification of wild collected mosquito vectors of diseases using gas chromatography-mass spectrometry in jazan province, saudi arabia.thirty-three species of mosquitoes have been reported from the kingdom of saudi arabia. several of these mosquitoes, anopheles gambiae giles s.l., anopheles stephensi liston, culex pipiens linnaeus, culex quinquefasciatus say, culex tritaeniorhynchus giles, stegomyia aegypti (linnaeus) and aedimorphus vexans arabiensis (patton) are known vectors of human and animal diseases. in this study, the cuticular hydrocarbon profiles of eight mosquito species using gas chromatography-mass spectrometry wer ...201324259205
a generic weather-driven model to predict mosquito population dynamics applied to species of anopheles, culex and aedes genera of southern france.an accurate understanding and prediction of mosquito population dynamics are needed to identify areas where there is a high risk of mosquito-borne disease spread and persistence. simulation tools are relevant for supporting decision-makers in the surveillance of vector populations, as models of vector population dynamics provide predictions of the greatest risk periods for vector abundance, which can be particularly helpful in areas with a highly variable environment. we present a generic weathe ...201525623972
study of culex tritaeniorhynchus and species composition of mosquitoes in a rice field in greece.mosquito species composition and seasonal abundance were studied in a rice field in western greece over a three-year period (2009-2011). a total of 11,716 larvae and pupae of mosquitoes were recorded, representing seven species, namely aedes caspius (pallas), anopheles hyrcanus (pallas), anopheles sacharovi favre, culex theileri theobald, culex tritaeniorhynchus giles, culex pipiens linnaeus, uranotaenia unguiculata edwards and belonging to four genera. cx. tritaeniorhynchus constituted the most ...201424613156
larval habitats diversity and distribution of the mosquito (diptera: culicidae) species in the republic of moldova.a countrywide field survey of immature mosquitoes was conducted in moldova with the aim to evaluate the culicidae species composition in different larval habitats and their distribution in the country. in total, 259 potential larval habitats were sampled in the 53 localities, resulting in 9,456 specimens. twenty species belonging to the genera anopheles, aedes, culex, culiseta, and uranotaenia were collected. mean species richness in aquatic habitats ranged from 1.00 to 4.00, and, for example, w ...201526364191
frequency tuning of individual auditory receptors in female mosquitoes (diptera, culicidae).the acoustic sensory organs in mosquitoes (johnston organs) have been thoroughly studied; yet, to date, no data are available on the individual tuning properties of the numerous receptors that convert sound-induced vibrations into electrical signals. all previous measurements of frequency tuning in mosquitoes have been based on the acoustically evoked field potentials recorded from the entire johnston organ. here, we present evidence that individual receptors have various frequency tunings and t ...201323742968
impact of physical and chemical characteristics of breeding sites on mosquito larval abundance at ismailia governorate, egypt.the distribution and monthly abundance of mosquito larvae in released water, drainage canals and sewage water tanks in ismailia governorate were investigated. the results obtained indicated the presence of five culicine (culex. pipiens, cx. pusillus, cx. perexiguus, cx. theleri and ochlerotatus. caspius) and two anopheline (anopheles. multicolor and an. pharoensis) mosquito species. significantly higher larval density was recorded in sewage water (n= 5534; 46.08%) as compared with released water ...201324260817
ecology of the mosquito larvae in urban environments of cairo governorate, egypt.mosquitoes were surveyed over one year period in two localities in cairo representing different levels of urban planning: el-muqattam (planned) and abu-seir (unplanned). culex pipiens, cx. perexiguus, cx. pusillus, ochlerotatus caspius, culiseta longiareolata and anopheles multicolor were the collected species at both sites. mosquitoes were more common in abu-seir than in el-muqattam, with larvae of the filaria vector cx. pipiens accounting for 81% and 52% of recorded individuals at these sites, ...201222662608
flight periodicities and vertical distribution of aedes cantons (mg.), ae. geniculatus (ol.), anopheles plumbeus steph. and culex pipiens l. (dipt., culicidae) in southern england. 197122894870
measure post-bloodmeal dispersal of mosquitoes and duration of radioactivity by using the isotope ³²p.the radioactive isotope (32)p-labeled disodium phosphate (na₂h(32)po₄) was injected via the jugular vein into a cow kept in a shed in maozhuang village, cao township of shanxian county, china. over the following 5 d, mosquitoes feeding on the cow were captured at distances up to 400 m to determine dispersal distance. the duration of radioactivity in the cow and marked mosquitoes was 10 d. the results showed that after blood feeding, anopheles sinensis and culex tritaeniorhynchus temporarily rest ...201425502034
identification of chemical constituents and larvicidal activity of kelussia odoratissima mozaffarian essential oil against two mosquito vectors anopheles stephensi and culex pipiens (diptera: culicidae).the larvicidal activity of essential oil extracted from an indigenous plant, kelussia odoratissima mozaffarian was evaluated against two mosquito species, anopheles stephensi and culex pipiens. the chemical composition of the essential oil obtained by hydrodistillation from branch tips and leaf of this plant was determined by gc and gc/ms analysis. forty-nine constituents were identified in the oil. the main constituents of the oil were z-ligustilide (77.73%), 2-octen-1-ol acetate (6.27%), e-lig ...201223022522
additive effects on the improvement of insecticidal activity: design, synthesis, and insecticidal activity of novel pymetrozine derivatives.a series of new pymetrozine analogues containing both methyl on the imine carbon and phenoxy group at the pyridine ring were designed and synthesized. their insecticidal activities against bean aphid (aphis craccivora), mosquito larvae (culex pipiens pallens), cotton bollworm (helicoverpa armigera), corn borer (ostrinia nubilalis) and oriental armyworm (mythimna separata) were evaluated. the results of bioassays indicated that most of the target compounds showed good insecticidal activity agains ...201626342545
design, synthesis, and insecticidal evaluation of new pyrazole derivatives containing imine, oxime ether, oxime ester, and dihydroisoxazoline groups based on the inhibitor binding pocket of respiratory complex i.on the basis of complex i receptor protein binding site and commercial tebufenpyrad and tolfenpyrad, four series of novel pyrazole-5-carboxamides containing imine, oxime ether, oxime ester, and dihydroisoxazoline were designed and synthesized via the key intermediate 4-chloro-3-ethyl-n-(4-formylbenzyl)-1-methyl-1h-pyrazole-5-carboxamide. the structures of target compounds were confirmed by ¹h nmr and high-resolution mass spectrum (hrms). the results of bioassays indicated that the target compoun ...201323972278
design, synthesis, and insecticidal activity of novel pyrazole derivatives containing α-hydroxymethyl-n-benzyl carboxamide, α-chloromethyl-n-benzyl carboxamide, and 4,5-dihydrooxazole moieties.on the basis of commercial insecticides tebufenpyrad and tolfenpyrad, two series of novel pyrazole-5-carboxamides containing α-hydroxymethyl-n-benzyl or α-chloromethyl-n-benzyl and pyrazoles containing 4,5-dihydrooxazole moieties were designed and synthesized via the key intermediate 2-amino-1-(4-substituted) phenyl ethanol. the structures of target compounds were confirmed by (1)h nmr and elemental analysis or high-resolution mass spectrum (hrms), and their activities against cotton bollworm (h ...201222304385
carboxylesterase-mediated insecticide resistance: quantitative increase induces broader metabolic resistance than qualitative change.carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (op) insecticides. carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (g/a151d) or from tryptophan to leucine at the 271 site (w271l), follo ...201526047115
response of primary vectors and related diseases to impoundment by the three gorges dam.to investigate the impact of the three gorges dam on the local ecological environment. we conducted a 3-year cross-sectional survey of natural focus infectious diseases in the area before and after the water level rose to 156 m to evaluate the dam's health impacts.201424684420
toxicity of some plant extracts against vector of lymphatic filariasis, culex pipiens.many insecticides are generally used as larvicides to control culex pipiens, vector of lymphatic filariasis. this study was undertaken to evaluate the larvicidal activity of some potential larvicidal plants extracts against c. pipiens larvae. the toxic effects of both ethanolic and petroleum ether plant extracts were evaluated under laboratory conditions against 3rd instar larvae of c. pipiens. forty ethanolic and petroleum ether extracts of 10 plants namely echinochloa stagninum, phragmites aus ...201526012233
effects of host sex, plant species, and putative host species on the prevalence of wolbachia in natural populations of bemisia tabaci (hemiptera: aleyrodidae): a modified nested pcr study.bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a globally distributed pest. one of the key endosymbionts in b. tabaci is wolbachia, an α-proteobacterium implicated in many important biological processes. previous studies indicated that the infection frequency of wolbachia in middle east-asia minor 1 (meam1) and mediterranean (med) varied greatly among populations in different areas. however, little is known about the factors that influence the prevalence of wolbachia in b. tabaci. in thi ...201526470122
laboratory evaluation of the effect of egyptian native plants against some parasitic vectors.four plant extracts possessing molluscicidal and insecticidal efficacy were evaluated under laboratory conditions versus biomphalaria alexandrina, lymnea cailliaudi snails, their egg masses and culex pipiens larvae. these extracts included grape seed, eucalyptus, pomegranate, verbesina alcoholic extracts, as well as eucalyptus oil.201223169160
molluscicidal and mosquitocidal activities of the essential oils of thymus capitatus hoff. et link. and marrubium vulgare l.steam distillation of essential oils of aerial parts of thymus capitatus and marrubium vulgare l. collected at north cost of egypt yielded 0.5% and 0.2%, respectively. results of gas chromatography-mass spectrometry analyses of the two samples identified 96.27% and 90.19% of the total oil composition for t. capitatus and m. vulgare, respectively. the two oil samples appeared dominated by the oxygenated constituents (88.22% for t. capitatus and 57.50% for m. vulgare), composed of phenols, mainly ...201222983292
a major facilitator superfamily protein encoded by tcmuck gene is not required for cuticle pigmentation, growth and development in tribolium castaneum.insect cuticle pigmentation and sclerotization (tanning) are vital physiological processes for insect growth, development and survival. we have previously identified several colorless precursor molecules as well as enzymes involved in their biosynthesis and processing to yield the mature intensely colored body cuticle pigments. a recent study indicated that the bombyx mori (silkmoth) gene, bmmuck, which encodes a protein orthologous to a culex pipiens quiquefasciatus (southern house mosquito) ci ...201424681434
blood meal identification of the mosquito (diptera: culicidae) specimens belong to culex pipiens complex that were collected from kayseri province.this study aimed to determine the host preferences in blood meal of specimens belonging to culex pipiens complex.201628091388
laboratory evaluation of differential attraction of culex pipiens pallens to fruit-based sugar baits.mosquito adults usually need to obtain sugar from floral nectaries and damaged fruits/seed pods to replenish their energy reserves. the newly developed attractive toxic sugar baits have been successfully applied in controlling various mosquito species outdoors. however, the attraction of culex pipiens pallens to different fruit-based sugar baits remains unknown. in the present study, we selected nine common fruit species, prepared the fruit-based sugar solutions, and investigated the attractiven ...201627456936
development of multifunctional metabolic synergists to suppress the evolution of resistance against pyrethroids in insects that blood feed on humans.pyrethroids are the insecticides of choice when exposure to humans is likely, such as occurs in vector and public-health-related control programs. unfortunately, the pyrethroids share a common resistance mechanism with dichlorodiphenyltrichloroethane (ddt), knockdown resistance (kdr), and prior extensive use of ddt has predisposed the pyrethroids to cross-resistance via kdr. given the widespread occurrence of kdr, the use of synergists with pyrethroids is considered to be prudent to guard agains ...201525044360
"singing in the tube"--audiovisual assay of plant oil repellent activity against mosquitoes (culex pipiens).plant essential oils have been suggested as a promising alternative to the established mosquito repellent deet (n,n-diethyl-meta-toluamide). searching for an assay with generally available equipment, we designed a new audiovisual assay of repellent activity against mosquitoes "singing in the tube," testing single mosquitoes in drosophila cultivation tubes. statistics with regression analysis should compensate for limitations of simple hardware. the assay was established with female culex pipiens ...201626412058
efficiency of colocasia esculenta leaves extract and histopathological effects on culex pipiens (diptera: culicidae).this study evaluated the toxicity of colocasia esculenta leaves extract on 3rd, 4th instars larvae and pupae of culex pipiens. bioassays showed that the 3rd instar larvae was the most susceptible to the different concentrations of extract, where the lc50 after 48 hr. post-exposure was 79.41, 109.65 & 141.25 for the 3rd, 4th instars larvae and pupal stage respectively. the histo-pathological effects of c. esculenta leaves extract on midgut regions and gastric caeca of the 3rd instar larvae were s ...201526012222
avian malaria infections in western european mosquitoes.in the complex life cycle of avian malaria parasites (plasmodium sp.), we still have a poor understanding on the vector-parasite relationships. this study described the community of potential avian malaria vectors in four portuguese reedbeds. we tested if their geographical distribution differed, and investigated on their plasmodium infections. the mosquitoes' feeding preferences were evaluated using co(2), mice, and birds as baits. the most abundant species were culex pipiens, culex theileri, a ...201222427023
oviposition strategies of florida culex (melanoconion) mosquitoes.in the genus culex, oviposition is often generalized as laying floating egg rafts directly on the surface of the water, as reported for arbovirus vectors culex pipiens l. and culex tarsalis coquillett, both members of subgenus culex. the current work reports on investigations of exceptions to this strategy by species of melanoconion, a diverse and mostly tropical subgenus of culex, with many arbovirus vectors. wild gravid females from vero beach, fl, were introduced into outdoor cages with open ...201728402498
[comparative characteristics of intergenic spacers of ribosomal rna gene cluster in mosquitoes of the genus culex (diptera: culicidae)].nucleotide sequences of intergenic spacer of ribosomal rna gene cluster (rigs) were identified in mosquitoes culex modestus, culex torrentium and culex pipiens pallens. the level of interpopulation variability of the rigs in the subspecies c. pipiens pipiens (form pipiens--mosquitoes that inhabit the open waters, and form molestus--mosquitoes that inhabit basements) living in russia was estimated. no extensive repetitive sequences characteristic of the rigs of all previously described species of ...201323888772
seasonal pattern of daily activity of aedes caspius, aedes detritus, culex modestus, and culex pipiens in the po delta of northern italy and significance for vector-borne disease risk assessment.the increasing concern about vector-borne diseases such as west nile disease in northern italy motivated our analysis of data on the mosquito fauna and the seasonal and daily flight patterns collected in 1998 in the po valley. collections were performed once a week from may to november, with human landing collections and co(2) traps. culex pipiens was present from july to october and showed a clearly unimodal nocturnal flight habit. culex modestus appeared in july-august and showed a bimodal fli ...201222548536
pirna-3312: a putative role for pyrethroid resistance in culex pipiens pallens (diptera: culicidae).piwi-interacting rnas (pirnas) are a newly identified class of small noncoding rnas. they are associated with chromatin organization, messenger rna stability, and genome structure. although the overexpression of pirna-3312 in deltamethrin-susceptible (ds) strain of culex pipiens pallens (l.) was observed in our previous large-scale transcriptome data, the roles of pirna in insecticide resistance have not been clearly defined. the aim of the present study was to investigate how pirna-3312 is invo ...201728399266
life table analysis of culex pipiens under simulated weather conditions in egypt.the effect of fluctuating ambient air and water temperatures and relative humidity (rh) on the population parameters of culex pipiens was studied using life table data during the winter-spring and summer-autumn seasons in giza province, egypt. in winter-spring (20.46 ± 1.8°c and 51.68 ± 3.63% rh), the preadult survival rate for cx. pipiens was 80.65%, which was higher than the 50.00% observed in the summer-autumn (30.19 ± 0.67°c and 52.68 ± 8.04% rh). during the winter-spring period, the develop ...201728388321
mosquito allergy in children: clinical features and limitation of commercially-available diagnostic tests.to determine the clinical features of mosquito allergy in children and the ability of commercially available mosquito allergy tests to detect children with mosquito allergy in thailand.201728364407
the role of mir-2∼13∼71 cluster in resistance to deltamethrin in culex pipiens pallens.excessive and continuous application of deltamethrin has resulted in the development of deltamethrin resistance among mosquitoes, which becomes a major obstacle for mosquito control. in a previous study, differentially expressed mirnas between deltamethrin-susceptible (ds) strain and deltamethrin-resistant (dr) strain using illumina sequencing in culex pipiens pallens were identified. in this study, we applied rnai and the centers for disease control and prevention (cdc) bottle bioassay to inves ...201728342977
toxicity of β-citronellol, geraniol and linalool from pelargonium roseum essential oil against the west nile and filariasis vector culex pipiens (diptera: culicidae).insect vectors are responsible for spreading devastating parasites and pathogens. a large number of botanicals have been suggested for eco-friendly control programs against mosquito vectors, and some of them are aromatic plants. pelargonium roseum, a species belonging to the geraniaceae family, due to its pleasant rose-like odor may represent a suitable candidate as mosquito repellent and/or larvicide. in this research, we evaluated the toxicity of the essential oil from p. roseum and its major ...201728297637
a novel rhabdovirus, related to merida virus, in field-collected mosquitoes from anatolia and thrace.next-generation sequencing technologies have significantly facilitated the discovery of novel viruses, and metagenomic surveillance of arthropods has enabled exploration of the diversity of novel or known viral agents. we have identified a novel rhabdovirus that is genetically related to the recently described merida virus via next-generation sequencing in a mosquito pool from thrace. the complete viral genome contains 11,798 nucleotides with 83% genome-wide nucleotide sequence similarity to mer ...201728283817
insecticide resistance and cytochrome-p450 activation in unfed and blood-fed laboratory and field populations of culex pipiens pallens.understanding the mechanisms of insecticide resistance to vector mosquitoes is critical for the implementation of effective control measures. a nulliparous susceptible culex pipiens pallens (kscp) laboratory colony and two field strains from paju (paj) and jeonju (jeo) korea were evaluated for susceptibility to five pesticides by microapplication techniques. unfed paj and jeo females demonstrated increased resistance compared to unfed kscp females, respectively. while blood-fed kscp females demo ...201728275327
comparative analysis of the circadian rhythm genes period and timeless in culex pipiens linnaeus, 1758 (diptera, culicidae).nucleotide sequences of the circadian rhythm genes, period and timeless, were studied for the first time in mosquitoes culex pipiens linnaeus, 1758. in this work we evaluated variations of the studied genome fragments for the two forms of culex pipiens (forma "pipiens" - mosquitoes common for aboveground habitats, forma "molestus" - underground mosquitoes). we compared culex pipiens from russia with transatlantic culex pipiens and subtropical culex quinquefasciatus say, 1823. our results show th ...201628123673
the diapause program impacts renal excretion and molecular expression of aquaporins in the northern house mosquito, culex pipiens.adult females of the mosquito culex pipiens entering diapause increase sugar water ingestion and reduce evaporative water loss, but how these attributes of the diapause program impact activity of the renal excretory system remains unknown. here we compared the renal excretory capacity of diapausing and non-diapausing females, as well as the molecular expression of aquaporin (aqp) genes that encode channels involved in transporting water and/or small metabolites. baseline urine excretion rates in ...201728034679
identification of disease-transmitting mosquitoes: development of species-specific probes for dna chip assay using mitochondrial coi and nd2 genes and ribosomal internal transcribed spacer 2.mosquitoes, which transmit infectious diseases, such as malaria and dengue fever, are harmful to human health. thus, accurate and rapid identification of vectors is a critical step for the control of mosquito-borne diseases. however, phenotypic variations in adults, lack of recognizable features of the immature, and fragility of mosquitoes make identification difficult. molecular approaches have been widely applied to identify mosquitoes, yet these methods have been focused only on the identific ...201728028142
a novel anticoagulant protein with antithrombotic properties from the mosquito culex pipiens pallens.a newly discovered protein, named 'cpp protein', which possesses antithrombotic and anticoagulant properties, was purified from the salivary gland of the mosquito culex pipiens pallens. cpp protein was found to have an estimated molecular mass of 21.7kda, and to be active at 60°c, and ph 7.0. the anticoagulation activity of cpp protein was strongly inhibited by calcium ions. cpp protein inhibited fibrin clot formation and platelet activation, and it degraded blood clots. it also inhibited the en ...201627554937
pyrethroid-resistance is modulated by mir-92a by targeting cpcpr4 in culex pipiens pallens.the wide use of pyrethroids has resulted in the emergence and spread of resistance in mosquito populations, which represent a major obstacle in the struggle against vector-borne diseases. resistance to pyrethroids is a complex genetic phenomenon attributed by polygenetic inheritance. we previously have sequenced and analyzed the mirna profiles of culex pipiens pallens. mir-92a was found to be overexpressed in a deltamethrin-resistant (dr) strain. the association of mir-92a with pyrethroid-resist ...201727627779
relating fitness to long-term environmental variations in natura.quantifying links between ecological processes and adaptation dynamics in natura remains a crucial challenge. many studies have documented the strength, form and direction of selection, and its variations in space and time, but only a few managed to link these variations to their proximal causes. this step is, however, crucial, if we are to understand how the variation in selective pressure affects adaptive allele dynamics in natural settings. we used data from a long-term survey (about 30 years ...201627662519
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