| corrigendum: assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | [this corrects the article on p. 760 in vol. 6, pmid: 26284045.]. | 2017 | 28450861 |
| rapid identification of pathogens responsible for necrotizing fasciitis on an integrated microfluidic system. | necrotic fasciitis (nf) is a particularly aggressive and serious infection of the fascia that can penetrate into the musculature and internal organs, resulting in death if not treated promptly. in this work, an integrated microfluidic system composed of micropumps, microvalves, and micromixers was used to automate the detection of pathogens associated with nf. the entire molecular diagnostic process, including bacteria isolation, lysis, nucleic acid amplification and optical detection steps, was ... | 2017 | 29282420 |
| correction: functional implications of hexameric assembly of rraa proteins from vibrio vulnificus. | [this corrects the article doi: 10.1371/journal.pone.0190064.]. | 2018 | 29352305 |
| melatonin inhibits apoptotic cell death induced by vibrio vulnificus vvha via melatonin receptor 2 coupling with ncf-1. | melatonin, an endogenous hormone molecule, has a variety of biological functions, but a functional role of melatonin in the infection of gram-negative bacterium vibrio vulnificus has yet to be described. in this study, we investigated the molecular mechanism of melatonin in the apoptosis of human intestinal epithelial (hct116) cells induced by the hemolysin (vvha) produced by v. vulnificus. melatonin (1 μm) significantly inhibited apoptosis induced by the recombinant protein (r) vvha, which had ... | 2018 | 29352110 |
| repression of vvpm protease expression by quorum sensing and the camp-crp complex in vibrio vulnificus. | septicemia-causing vibrio vulnificus produces at least three exoproteases, vvpe, vvps and vvpm, all of which participate in interactions with human cells. expression of vvpe and vvps is induced in the stationary phase by multiple transcription factors including sigma s, smcr, and camp-crp complex. distinct roles of vvpm, such as induction of apoptosis, lead us to hypothesize vvpm expression is different from that of the other exoproteases. its transcription, which was found to be independent of ... | 2018 | 29339417 |
| role of heat shock proteases in quorum-sensing-mediated regulation of biofilm formation by vibrio species. | capsular polysaccharide (cps) is essential for the dispersal of biofilms formed by the pathogenic bacterium vibrio vulnificus cps production is induced by the quorum-sensing (qs) master regulator smcr when biofilms mature. however, v. vulnificus biofilms formed under heat shock conditions did not exhibit the dispersion stage. transcripts of the cps gene cluster were at basal levels in the heat-exposed cell owing to reduced cellular levels of smcr. at least two proteases induced by heat shock, cl ... | 2018 | 29295912 |
| functional implications of hexameric assembly of rraa proteins from vibrio vulnificus. | rnase e has a pivotal role in the degradation and processing of rnas in escherichia coli, and protein inhibitors rraa and rrab control its enzymatic activity. the halophilic pathogenic bacterium vibrio vulnificus also expresses orthologs of rnase e and rraa-rnase ev, rraav1, and rraav2 (herein renamed as vvrnase e, vvrraa1, and vvrraa2). a previous study showed that vvrraa1 actively inhibits the ribonucleolytic activity of vvrnase e by interacting with the c-terminal region of vvrnase e. however ... | 2017 | 29261778 |
| transcriptome data of epinephelus fuscoguttatus infected by vibrio vulnificus. | vibriosis disease by vibrio spp. greatly reduced productivity of aquaculture, such as brown-marbled grouper (epinephelus fuscoguttatus), which is an economically important fish species in malaysia. preventive measures and immediate treatment are critical to reduce the mortality of e. fuscoguttatus from vibriosis. to investigate the molecular mechanisms associated with immune response and host-bacteria interaction, a transcriptomic analysis was performed to compare between healthy and vibrio-infe ... | 2018 | 29255779 |
| a conserved tad pilus promotes vibrio vulnificus oyster colonization. | vibrio vulnificus has the highest death rate (>35%) and per-case economic burden ($3.3 million) of any foodborne pathogen in the united states. infections occur via open wounds or following ingestion of contaminated seafood, most infamously oysters. we isolated a 1000th generation descendant, designated nt that exhibited increased biofilm and aggregate formation relative to its parent. we identified two significant causal changes underlying these phenotypes. first, the entire 24-kb capsular poly ... | 2017 | 29235706 |
| [amputation of an arm due to infection with vibrio vulnificus after beach holiday]. | infection with vibrio vulnificus is a rare condition with approximately 11 cases reported in the danish literature. it is characterized by soft tissue infection/wound, necrotizing fasciitis and septicaemia. in this case report we present a patient admitted with a rapid progression and sepsis consistent with v. vulnificus infection but with no informa-tion of water exposure. the initial treatment was surgery and sepsis management including broad-spectrum antibiotics. on day eight the patient's ri ... | 2017 | 29208199 |
| complete genome sequence of the vibrio vulnificus strain vv2014djh, a human-pathogenic bacterium isolated from a death case in china. | vibrio vulnificus, an opportunistic pathogen, is the causative agent of life-threatening septicemia and severe wound infections. however, the pathogenicity and virulence factors of v. vulnificus are not fully understood. here we report the complete genome sequence of v. vulnificus vv2014djh, which was isolated from a death case. | 2017 | 29201149 |
| analysis of the bacterial microbiome in the small octopus, octopus variabilis, from south korea to detect the potential risk of foodborne illness and to improve product management. | the small octopus (octopus variabilis) is a popular seafood in many countries including south korea. because it is often consumed uncooked, the microorganisms in it often cause food poisoning. therefore, analyzing the microbiome of the small octopus can help to understand the risk of food poisoning and manage octopus products better. a total of 40 small octopuses were collected from four sites in november and august. the microbiota was analyzed using illumina miseq sequencing, and the amount of ... | 2017 | 29195979 |
| direct detection of vibrio vulnificus, vibrio parahaemolyticus, and vibrio alginolyticus from clinical and environmental samples by a multiplex touchdown polymerase chain reaction assay. | vibrio vulnificus, v. alginolyticus, and v. parahaemolyticus are commonly and opportunistically pathogenic to humans. | 2018 | 29173069 |
| bioactive pimarane diterpenes from the arctic fungus eutypella sp. d-1. | two new pimarane diterpenes, libertellenone m (1) and libertellenone n (2), together with five known compounds were isolated from the culture extract of eutypella sp. d-1 derived from high-latitude soil of the arctic. the structures of these compounds were determined by spectroscopic data as well as experimental and calculated electronic circular dichroism (ecd) analysis. antimicrobial and cytotoxic activities of the isolated compounds were evaluated. compound 3 exhibited weak antibacterial acti ... | 2017 | 29168349 |
| the opportunistic pathogen vibrio vulnificus produces outer membrane vesicles in a spatially distinct manner related to capsular polysaccharide. | vibrio vulnificus, a bacterial species that inhabits brackish waters, is an opportunistic pathogen of humans. v. vulnificus infections can cause acute gastroenteritis, invasive septicemia, tissue necrosis, and potentially death. virulence factors associated with v. vulnificus include the capsular polysaccharide (cps), lipopolysaccharide, flagellum, pili, and outer membrane vesicles (omvs). the aims of this study were to characterize the morphology of v. vulnificus cells and the formation and arr ... | 2017 | 29163452 |
| vibrio parahaemolyticus and vibrio vulnificus recovered from oysters during an oyster relay study. | vibrio parahaemolyticus and vibrio vulnificus are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the united states. though multiple-antibiotic-resistant v. parahaemolyticus and v. vulnificus strains have been reported, resistance patterns in vibrios are not as well documented as those of other foodborne bacterial pathogens. salinity relaying (sr) is a postharvest processing (php) treatment to reduce the abundances of these path ... | 2018 | 29150510 |
| immunogenicity and protective efficacy of vibrio vulnificus flagellin protein flab in a wound infection model. | vibrio vulnificus is known as an opportunistic bacterial pathogen that causes primary septicemia and wound infection in humans. recently, the incidence of wound infection by v. vulnificus is increasing in warm countries. in this study, we examined a vaccine antigen against v. vulnificus in mice. flab, a component protein of the v. vulnificus flagellum, was expressed as a recombinant protein, named rflab. after immunization of mice with rflab, the mice were challenged by subcutaneous inoculation ... | 2018 | 29142160 |
| vibrio vulnificus infection induces the maturation and activation of dendritic cells with inflammatory th17-polarizing ability. | vibrio vulnificus (v. vulnificus) is a gram-negative bacterium, which causes life-threatening septicemia and gastroenteritis through the consumption of contaminated seafood or wound infection. in addition, v. vulnificus infection is known to stimulate the production of several pro-inflammatory cytokines, which are associated with inflammatory responses mediated predominantly by dendritic cells (dcs), functioning as antigen-presenting cells. the present study aimed to investigate whether v. vulni ... | 2018 | 29115398 |
| cytotoxic and antibacterial compounds from the coral-derived fungus aspergillus tritici sp2-8-1. | three novel compounds, 4-methyl-candidusin a (1), aspetritone a (2) and aspetritone b (3), were obtained from the culture of a coral-derived fungus aspergillus tritici sp2-8-1, together with fifteen known compounds (4-18). their structures, including absolute configurations, were assigned based on nmr, ms, and time-dependent density functional theory (td-dft) ecd calculations. compounds 2 and 5 exhibited better activities against methicillin-resistant strains of s. aureus (mrsa) atcc 43300 and m ... | 2017 | 29112138 |
| antimicrobial activity of a newly identified kazal-type serine proteinase inhibitor, cckpi1, from the jellyfish cyanea capillata. | in this study, we reported a jellyfish-derived kazal-type serine protease inhibitor, named cckpi1, from cyanea capillata. cckpi1 has a calculated molecular mass of 19.02kda and contains three typical kazal domains. soluble recombinant cckpi1 (rcckpi1) was successfully expressed and purified. rcckpi1 exhibited significant inhibitory activities against elastase, subtilisin a and proteinase k, but not against trypsin or chymotrypsin. kinetic studies showed that all of the inhibitory effects of rcck ... | 2018 | 29054522 |
| antimicrobial peptide-loaded gold nanoparticle-dna aptamer conjugates as highly effective antibacterial therapeutics against vibrio vulnificus. | vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against v. vulnificus infection. however, the therapeutic effects of antibiotics are limited by multidrug resistance. in this study, we demonstrated that an antimicrobial peptide (amp), hpa3phis, loaded onto a gold nanoparticle-dna aptamer (aunp-apt) conjugate (aunp-apt-hpa3phis) is an effective therapeutic tool against v. vulnificus infection in vivo in mice. hpa3phis induced bacterial c ... | 2017 | 29051620 |
| isolation, identification and phenotypic and molecular characterization of pathogenic vibrio vulnificus isolated from litopenaeus vannamei. | the morphology and the drug sensitivity of the strain gyx2014-1 isolated from the hepatic pancreatic tissue of moribund litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. detection of extracellulase and hemolysin activity shows that the isolated gyx2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. the 16s rrna gene (genbank accession number: kt781675) was ... | 2017 | 29045415 |
| vibrio vulnificus: new insights into a deadly opportunistic pathogen. | vibrio vulnificus is a gram-negative aquatic bacterium first isolated by the united states (us) centers for disease control and prevention (cdc) in 1964. this bacterium is part of the normal microbiota of estuarine waters and occurs in high numbers in molluscan shellfish around the world, particularly in warmer months. infections in humans are derived from consumption of seafood produce and from water exposure. vibrio vulnificus is a striking and enigmatic human pathogen, yet many aspects relate ... | 2017 | 29027375 |
| a case of vibrio vulnificus infection complicated with fulminant purpura: gene and biotype analysis of the pathogen. | introduction.vibrio vulnificus (v. vulnificus) causes a severe infection that develops in the compromised host. its pathophysiology is classified into three types: (1) primary septicaemia, (2) gastrointestinal illness pattern and (3) wound infection pattern. of these, primary septicaemia is critical. v. vulnificus can be classified into three biotypes and two genotypes and its pathogenicity is type-dependent. case presentation. a 47-year-old man presented to a local hospital with chief complaint ... | 2017 | 29026623 |
| efficacy of ceftriaxone, cefepime, doxycycline, ciprofloxacin, and combination therapy for vibrio vulnificus foodborne septicemia. | foodborne vibrio vulnificus infections are associated with higher rates of sepsis and mortality than wound infections; however, antibiotic efficacy studies have not been performed in foodborne infection models. the efficacies of ceftriaxone, cefepime, doxycycline, ciprofloxacin, and combination therapy were assessed in v. vulnificus intestinal infection in mice in order to model foodborne infections. in accordance with prior studies of cefotaxime, cefepime was synergistic with doxycycline and ci ... | 2017 | 28971862 |
| rapid biosynthesized agnps from gelidiella acerosa aqueous extract mitigates quorum sensing mediated biofilm formation of vibrio species-an in vitro and in vivo approach. | the present study explores the non-bactericidal anti-virulence efficacy of green synthesized silver nanoparticles (agnps) from gelidiella acerosa against multi-drug resistant vibrio spp. spectral characterization of agnps was performed through uv-visible, ft-ir, and energy-dispersive spectroscopic techniques followed by x-ray crystallography and zeta potential analysis. further, the structural characterization was done by electron and atomic force microscopic techniques. agnps profoundly quelled ... | 2017 | 28965300 |
| vibrio species involved in seafood-borne outbreaks (vibrio cholerae, v. parahaemolyticus and v. vulnificus): review of microbiological versus recent molecular detection methods in seafood products. | seafood products are widely consumed all around the world and play a significant role on the economic market. bacteria of the vibrio genus can contaminate seafood and thus pose a risk to human health. three main vibrio species, v. cholerae, v. parahaemolyticus and v. vulnificus, are potentially pathogenic to humans. these species are responsible for a dramatic increase of seafood-borne infections worldwide. hence, early detection of total and pathogenic vibrio is needed and should rely on quick ... | 2017 | 28956623 |
| occurrence of virulence genes associated with human pathogenic vibrios isolated from two commercial dusky kob (argyrosmus japonicus) farms and kareiga estuary in the eastern cape province, south africa. | background: seafood-borne vibrio infections, often linked to contaminated seafood and water, are of increasing global public health concern. the aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and kareiga estuary, south africa. methods: a total of 200 samples including dusky kob fish (n = 120) and seawater (n = 80) were subjected to vibrio screening on thiosulf ... | 2017 | 28946684 |
| morphological characterization and biocontrol effects of vibrio vulnificus phages against vibriosis in the shrimp aquaculture environment. | the re-emerging field of phage therapy is the potential biocontrol agents for the transfer of virulence factor and to kill their bacterial hosts. in this study, the lytic vibrio vulnificus phages were studied to provide a better understanding of phage-host interactions and development of phage therapy. four new v. vulnificus phages were detected from shrimp aquaculture system, named vv1, vv2, vv3 and vv4. all lytic v. vulnificus phages are the tectiviruses of the family tectiviridae with typical ... | 2017 | 28917670 |
| biochemical characterization of a catalase from vibrio vulnificus, a pathogen that causes gastroenteritis. | vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. most v. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. in the study presented here, a catalase from v. vulnificus (cat-vv) was purified to homogeneity after expression in escherichia coli. the kinetics and function of cat-vv were examined. cat-vv catalyzed the reduction of h2o2 at an optimal ph of 7.5 and tempe ... | 2017 | 28892106 |
| proteomics and 1h nmr-based metabolomics analysis of pathogenic vibrio vulnificus aquacultures isolated from sewage drains. | vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. vibrio vulnificus is a pathogenic bacterium that infects people and livestock. it is usually found in offshore waters or within fish and shellfish. this study presents a comparative proteomic analysis of the outer membrane protein (omp) changes in v. vulnificus proteins after stimulation with sewage from sewage drains. using two-dimensional electrophoresis followed by maldi-tof ms/ms, 32 protein ... | 2017 | 28864971 |
| exogenous polyunsaturated fatty acids impact membrane remodeling and affect virulence phenotypes among pathogenic vibrio species. | the pathogenic vibrio species (v. cholerae, v. parahaemolyticus, and v. vulnificus) represent a constant threat to human health, causing foodborne and skin wound infections as a result of ingestion of or exposure to contaminated water and seafood. recent studies have highlighted vibrio's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. the possession and conservation of such machinery provokes consideration of fatty acids as important factors in ... | 2017 | 28864654 |
| complete genome sequence of the pathogenic vibrio vulnificus type strain atcc 27562. | vibrio vulnificus has the highest death rate and economic burden per case of any foodborne pathogen in the united states. a complete genome sequence of the type strain promotes comparative analyses with other clinical and environmental isolates, improving our understanding of this important human pathogen and successful environmental organism. | 2017 | 28860258 |
| partial characterization of bacteriocin produced by halotolerant pediococcus acidilactici strain qc38 isolated from traditional cotija cheese. | during a screening of lactic acid bacteria producing bacteriocin from cotija cheese, the strain qc38 was isolated. based on the 16s rrna gene nucleotide sequencing (516 pb accession no kj210322) and phylogenetic analysis, the isolate was identified as pediococcus acidilactici. neutralized cell-free supernatant was tested for antimicrobial activity against 17 gram-negative and gram-positive pathogens. growth inhibition was achieved against listeria monocytogenes (supplier or indication or source) ... | 2016 | 29334047 |
| to17: a teleost antimicrobial peptide that induces degradation of bacterial nucleic acids and inhibits bacterial infection in red drum, sciaenops ocellatus. | tissue factor pathway inhibitor (tfpi)-1 is well known for its role as an inhibitor of blood coagulation. several studies have demonstrated that the c-terminal peptides of tfpi-1 are active against a broad spectrum of microorganisms. in a previous study, we found that to17 (with 17 amino acids), a tfpi-1 c-terminal peptide from red drum (sciaenops ocellatus), was active against edwardsiella tarda. in the present study, we investigated further the antimicrobial spectrum, action mode, as well as t ... | 2018 | 29183811 |
| wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution. | fish skin mucosal surfaces (sms) are quite similar in composition and function to some mammalian ms and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. we aimed to test this hypothesis by searching for metagenomic and genomic evidences in the sms-microbiome of a model fish species (anguilla anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the ... | 2017 | 29268781 |
| crystal structure of peroxiredoxin 3 from vibrio vulnificus and its implications for scavenging peroxides and nitric oxide. | peroxiredoxins (prxs) are ubiquitous cysteine-based peroxidase enzymes. recently, a new type of prx, vvprx3, was identified in the pathogenic bacterium vibrio vulnificus as being important for survival in macrophages. it employs only one catalytic cysteine residue to decompose peroxides. here, crystal structures of vvprx3 representing its reduced and oxidized states have been determined, together with an h2o2-bound structure, at high resolution. the crystal structure representing the reduced prx ... | 2018 | 29354274 |