| localization of adriamycin in model and natural membranes. influence of lipid molecular packing. | the interaction of adriamycin with lipids was studied in model (monolayers, small unilamellar vesicles, large multilamellar vesicles) and natural (chinese hamster ovary cell) membranes by measurement of fluorescence energy transfer and fluorescence quenching. 2-apam, 7-aste, 12-aste and anthracene-phosphatidylcholine were used as fluorescent probes in which the anthracene group is well located at graded depths in the membrane. egg-yolk phosphatidylcholine and a 1/1 mixture of it with bovine brai ... | 1989 | 2731543 |
| comparison of the reactivities and immunogenicities of procholeragenoid and the b subunit of cholera toxin in thai volunteers. | procholeragenoid, a stable high-molecular-weight aggregate of cholera toxin derived by heat treatment, was evaluated for reactivity and immunogenicity in adult thai volunteers. since procholeragenoid is known to retain some residual activity of cholera toxin, increasing amounts were ingested until diarrhea occurred; 250 micrograms induced diarrhea, but 100 micrograms did not. procholeragenoid and cholera toxin b subunit, both in 100-micrograms amounts, were then compared for systemic and intesti ... | 1989 | 2731977 |
| milk plasma supports growth and differentiation of a mouse teratocarcinoma cell line. | | 1989 | 2732192 |
| improved procedure for preparation of f(ab')2 fragments of mouse iggs by papain digestion. | preparation of f(ab')2 fragments from mouse monoclonal igg by papain digestion can result in incorporation of traces of papain into antibody fragments by disulfide exchange. such trace contamination can have detrimental effects on the integrity of these antibody fragments following reduction. gel filtration and ion exchange chromatography procedures did not eliminate papain contamination from f(ab')2 preparations. the use of antibody specific for papain to remove this contamination from f(ab')2 ... | 1989 | 2732475 |
| vibrio cholerae o1 adherence to human small intestinal m cells in vitro. | | 1989 | 2732514 |
| [microfilaments and intermediate filaments in the fetal capillary endothelial cells of the human chorionic villi--ultrastructural and immunohistochemical study]. | augmentation of cytoskeletons in fetal capillary endothelial cells is one of the well known pathologic changes in toxemic placentae. but we can find very few reports on these filaments in placentae, especially on their ontogeny. an ultrastructural study on human chorionic villi from 55 normal and 25 toxemic pregnancies, covering all periods of gestation, revealed that endothelial cells already had a small amount of intermediate filaments and microfilaments from 6 weeks' gestation and that they g ... | 1989 | 2732559 |
| turning off the diarrhea: the role of food and ors. | ninety-three boys aged 5 years or less who had diarrhea due to vibrio cholerae were randomly assigned to treatment with glucose oral rehydration salt (ors) or rice-based ors. for the first 24 h, ors only was given to all the patients. during the next 24 h, ors and normal food were given. the efficacy of the two types of ors was compared in terms of ors intake, stool output, change in hematocrit reading, serum specific gravity, and increase in body weight. at the end of the first 24 h of treatmen ... | 1989 | 2732867 |
| kinetic experiments with radionuclides concerning the perilymph-blood barrier in a guinea pig model. | since 1950 many animal radiotracer experiments have been performed to study inner ear kinetics. for the most part in these studies, radionuclides were applied systemically, following which a discontinuous probing of inner ear fluids or of inner ear tissues was done. two techniques have been developed in the section for experimental otorhinolaryngology of the university of würzburg. these have been adapted to the direct and continuous measurements of inner ear efflux kinetics for several hour per ... | 1989 | 2735826 |
| allyl alcohol-induced hemolysis and its relation to iron release and lipid peroxidation. | allyl alcohol administration to starved mice produced, along with liver necrosis, a high incidence (about 50%) of hemolysis. a marked decrease in erythrocyte glutathione (gsh) was seen in all the intoxicated animals. such a decrease was significantly higher in the animals showing hemolysis. in these animals a substantial amount of malonic dialdehyde (mda) was detected in plasma and a marked decrease in arachidonic and docosahexaenoic acids was found in erythrocyte phospholipids. these data sugge ... | 1989 | 2735939 |
| metabolism of triiodothyroacetic acid (ta3) in rat liver. ii. deiodination and conjugation of ta3 by rat hepatocytes and in rats in vivo. | the hepatic metabolism of 3,3',5-triiodothyroacetic acid (ta3), a naturally occurring side-chain analog of t3, was studied in vitro and in vivo. metabolites were quantified by hplc after sephadex lh-20 prepurification of samples obtained after incubation of [125i]ta3 or 3,[3'-125i]diiodothyroacetic acid (3,[3'-125i]ta2) with isolated rat hepatocytes under various conditions or after iv administration of [125i]ta3 to normal or 6-propyl-2-thiouracil (ptu)-treated rats. in protein-free incubations ... | 1989 | 2737157 |
| heterogeneity of fast-oxidative muscle fibers of chicken demonstrated by anti-myosin monoclonal antibodies. | the fiber type composition of two fast muscles of the chicken, namely, adductor superficialis (as) and pectoralis major (pm) was examined by the histochemical myosin atpase staining and immunochemical techniques using monoclonal antibodies (mcabs). two new mcabs produced against the myosin of the anterior latissimus dorsi (ald) muscle of the chicken and named ald-122 and ald-83 were characterized to be specific for myosin heavy chain (mhc) and for myosin light chain-1 respectively. they were use ... | 1989 | 2737924 |
| isolation of streptomyces tendae mutants with an altered nikkomycin spectrum. | to isolate streptomyces tendae mutants blocked in the biosynthesis of the nikkomycin nucleoside base 4-formyl-4-imidazoline-2-one, an assay was developed to detect the formation of nikkomycins containing this base during growth on solid medium. the assay is based on the reaction of the 4-formylimidazolone structure of nikkomycins with the aldehyde reagent barbituric acid leading to red-colored products. among 18,000 n-methyl-n'-nitro-n-nitrosoguanidine treated clones tested in the barbituric aci ... | 1989 | 2737950 |
| stimulatory effect of cefodizime on macrophage-mediated phagocytosis. | we evaluated the ingestion of anti-sheep erythrocyte (anti-e) igg- and igm-coated sheep erythrocytes by murine peritoneal macrophages exposed to cefodizime, a new semisynthetic cephalosporin, and other antibiotics. cefodizime enhanced the ingestion of anti-e igg-coated erythrocytes by peritoneal macrophages from cd-1 and balb/c mice in a dose-dependent manner, but had no effect on uncoated or igm-coated erythrocytes. similar enhancement was observed only in the case of cefpimizole (ac-1370), amo ... | 1989 | 2737956 |
| identification of human apolipoprotein e variant gene: apolipoprotein e7 (glu244,245----lys244,245). | apolipoprotein e (apoe) is one of the protein moieties of the human serum lipoproteins. three major isoforms of apoe (apoe2, apoe3, and apoe4) and minor variant isoforms (apoe1, apoe5, and apoe7) have been detected by isoelectric focusing. in this study we have cloned the apoe7 gene from a patient with the apoe3/e7 phenotype associated with hypertriglyceridemia and diabetes mellitus. dna sequencing revealed that the apoe7 gene has two base substitutions (g----a) changing glu244,245----lys244,245 ... | 1989 | 2738044 |
| ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart. | cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ... | 1989 | 2738265 |
| end-to-side and end-to-end vascular anastomoses with a carbon dioxide laser. | this study was designed to compare anastomoses performed with a carbon dioxide laser and conventional anastomoses performed with 7-0 polypropylene suture. in each of 80 rabbits, the divided left carotid artery was anastomosed by a continuous suture technique and the right carotid was anastomosed with a carbon dioxide laser. in each of 40 additional rabbits, both end-to-end and end-to-side laser anastomoses were performed on the same carotid artery. the laser technique involved the placement of t ... | 1989 | 2739426 |
| nocardial spinal epidural abscess. | | 1989 | 2739931 |
| [articular involvement in swiss patients with a borrelia burgdorferi infection--report of 8 cases]. | eight cases of lyme's arthritis are reported. in four of them, they are related to an erythema chronicum migrans, in two to an acrodermatitis atrophicans. in two cases, there was no cutaneous manifestation, but the serology was strongly positive for borrelia burgdorferi, and there was rapid relief of the pain with antibiotic therapy. it is suggested that lyme's arthritis is not rare in switzerland and that it must be searched for in every case of arthritis of unknown etiology, especially when th ... | 1989 | 2740677 |
| glycaemic effects of traditional european plant treatments for diabetes. studies in normal and streptozotocin diabetic mice. | twelve plants used for the traditional treatment of diabetes mellitus in northern europe were studied using normal and streptozotocin diabetic mice to evaluate effects on glucose homeostasis. the plants were administered in the diet (6.25% by weight) and/or as decoctions or infusions in place of drinking water, to coincide with the traditional method of preparation. treatment for 28 days with preparations of burdock (arctium lappa), cashew (anacardium occidentale), dandelion (taraxacum officinal ... | 1989 | 2743711 |
| cartilage content of an immature dog. | the near total mineral-free and elastin-free cartilage content of a three-month-old mongrel dog was 0.73% of the dry body weight. extraarticular (costal, tracheobronchial, thyroid, cricoid, nasal septum) cartilage constituted 0.44%; articular, 0.06; intervertebral disc, 0.10; elastic, 0.12 and meniscal fibrocartilage, 0.01%. articular tissue accounted for only 7.25% of the total cartilage uronic acid. this is the first estimate of the amount and distribution of cartilage in a vertebrate species. | 1989 | 2743766 |
| [induction with cadmium of lipid peroxidation in the tissues of albino rats and its prevention with ascorbic acid]. | | 1989 | 2744514 |
| synaptic cytoarchitecture of the cat septum pellucidum. | | 1989 | 2747886 |
| electrophysiological analysis of the hippocampal projections to the entorhinal area. | responses evoked in the entorhinal area by impulse volleys originating in the ipsilateral hippocampus were analysed in the guinea-pig by means of field potential analysis. perforant path volleys, synaptically elicited by stimulation of the dorsal psalterium of one side, were used to activate the hippocampal lamellar circuit of the same side and, through interhippocampal impulses, the hippocampal pyramidal neurons of the contralateral side. discharge of the hippocampal pyramidal neurons was follo ... | 1989 | 2747915 |
| connectivity of striatal grafts implanted into the ibotenic acid-lesioned striatum--ii. cortical afferents. | grafts of fetal striatal tissue, implanted into the ibotenic acid-lesioned caudate-putamen in adult recipient rats, have previously been shown to receive extensive afferent inputs from various subcortical areas in the host. in the present study, the formation of graft afferents from the host neocortex has been analysed, 3-12 months after transplantation, by means of the anterograde axonal tracer phaseolus vulgaris leucoagglutinin, iontophoretically injected at multiple sites into the host fronta ... | 1989 | 2747919 |
| oral cholera vaccines containing b-subunit-killed whole cells and killed whole cells only. i. cross-reacting antigens of members of family vibrionaceae and the vaccines. | antigenic similarity between the oral cholera b subunit-whole cell (bs-wc) and whole-cell only (wc) vaccines and strains of the family vibrionaceae was studied by crossed immunoelectrophoresis. a reference system consisting of vibrio cholerae inaba e1 tor antigenic extract and homologous rabbit antiserum was applied in the study. the system was represented by 30 anodically migrating antigens forming distinct precipitation bands. antigenic extracts of other members of the family vibrionaceae show ... | 1989 | 2750268 |
| time-resolved extrinsic fluorescence of aromatic-l-amino-acid decarboxylase. | the coenzyme-linked fluorescence of aromatic-l-amino-acid decarboxylase decays non-exponentially. the decay of both native and nabh4 reduced samples can only be fitted by two exponentials each roughly accounting for about half of the total fluorescence. denaturation of the reduced protein with 8 m urea makes the fluorescence decay mono-exponential, like that observed for the reference compound pyridoxamine-5-phosphate. an extra pyridoxyl moiety can be bound to the enzyme after incubation with ex ... | 1989 | 2752045 |
| [yersiniosis]. | | 1989 | 2752462 |
| pulmonary toxicity of recombinant human tumor necrosis factor. | tumor necrosis factor is a cytokine produced by activated macrophages that causes hemorrhagic necrosis in various tumours. in preliminary clinical trials, patients have developed various degrees of respiratory insufficiency following administration of rtnf. twenty-seven patients were studied prospectively to evaluate the effect of administration of rtnf on pulmonary function. sixteen of the 27 patients completed the eight-week course of daily im injection of rtnf. spirometric data and dsb were m ... | 1989 | 2752816 |
| [transplantability and immunogenicity in mice of lymphoma nk/ly cells modified by concanavalin a and neuraminidase]. | the modifying effect of con a, vibrio cholerae and non-vibrio cholerae neuraminidase (vcn, nvcn) on the proliferative potential and immunogenicity of experimental lymphoma nk/ly cells is shown in vitro and in vivo. the efficacy of immunization by the modified tumour cells of mice with transplantable lymphoma is evaluated. a considerable decrease in the proliferative potential of transplantability and a moderate increase in immunogenicity of con a treated cells were determined in vitro. the high ... | 1989 | 2752940 |
| further studies on the role of the gonads in determining the ontogeny of gonadotropin secretion in the guinea pig (cavia porcelus). | to further examine whether nongonadal restraint of gonadotropin secretion during prepubertal development plays a major role in the timing of puberty in the guinea pig, as it does in the monkey and other primates, two experiments were performed. in the first, neonatal male and female guinea pigs were either bilaterally gonadectomized or sham gonadectomized on day 2 of age, or left intact. circulating lh concentrations were measured in blood samples collected by cardiac puncture every 4 days begin ... | 1989 | 2752984 |
| exposure level monitor of 3-amino-1,4-dimethyl-5h-pyrido[4,3-b]indole, a dietary carcinogen, in rabbits. | the present investigation describes a method for the detection of minute amounts of 3-amino-1,4-dimethyl-5h-pyrido[4,3-b]indole (trp-p-1), a carcinogenic tryptophan pyrolysate, bound to the hemoglobin of erythrocytes and plasma from rabbits dosed orally with the dietary carcinogen. the method consists of the acid-induced release of the dietary carcinogen adducts as the free carcinogen and their extraction with methylene chloride and subsequent quantitation by high-performance liquid chromatograp ... | 1989 | 2753027 |
| duration of inhibition of immediate skin test reactivity by hydroxyzine hydrochloride in dogs. | the duration of hydroxyzine-mediated suppression of the immediate hypersensitivity reaction in the skin of dogs was assessed by intradermal administration of various dilutions of histamine phosphate and of aqueous flea antigen in 18 dogs known to be allergic to fleas. wheal diameters and scores were used to evaluate the strength of the resulting reactions. in most dogs, significant (p less than 0.05) inhibition lasted from 3 to 5 days after withdrawal from treatment. some dogs took up to 9 days ... | 1989 | 2753775 |
| ascorbic acid facilitates chicken myoblast fusion in vitro. | addition of low concentrations of ascorbic acid (5 micrograms/ml to 10 micrograms/ml) to myogenic chick embryo cultures resulted in an early fusion. at 30 h cultures receiving small amounts of ascorbic acid presented fusion rates 3 times that of the control. however, control rates of fusion were not different from those of experimentals at 50 h. no such effect was seen with ascorbic acid added at 24 h of culture, or with ascorbic acid addition to a calcium-deprived system. these findings demonst ... | 1989 | 2753849 |
| staphylococcus aureus strains which are not identified by rapid agglutination methods are of capsular serotype 5. | a total of 183 recent staphylococcus aureus clinical isolates were tested with three commercially available rapid agglutination methods. the capsular polysaccharide type and resistance to oxacillin of these isolates were also determined. seven isolates were not identified correctly by agglutination methods. all isolates not identified by the rapid methods were of capsular serotype 5, and of these isolates, six were resistant to oxacillin. the results suggest that these agglutination kits can be ... | 1989 | 2754004 |
| on the asymmetry of the primary branching of vagal sensory axons: possible role of the supporting tissue. | central and peripheral nonmedullated processes of vagal nodosal neurons of the cat were studied in normal nerves and after regeneration along their anatomical course and along the hypoglossal nerve. nonmedullated fibers above the ganglion and in the root had comparable sizes (approximately 0.37 micron2) and caliber distribution. below the ganglion, the cross-sectional area increased to 1.0 micron2. in axons of equal caliber, supranodosal and radicular fibers had similar microtubular densities wh ... | 1989 | 2754026 |
| retinal projections to the medial terminal nucleus of the rat with induced unilateral congenital eye defects. | retinal projections, particularly the ipsilateral projections, to the medial terminal nucleus (mtn) of the accessory optic system were investigated by autoradiography in adult rats after being reared in one of three different conditions: (1) normal visual experiences, (2) an application of trypan blue for intrauterine induction of congenital unilateral anophthalmia; and (3) the procedures for intrauterine production of congenital microphthalmia in which both eyes are reduced in size. in congenit ... | 1989 | 2754416 |
| antiulcer agents. 4. conformational considerations and the antiulcer activity of substituted imidazo[1,2-a]pyridines and related analogues. | definition of the interrelationship between the conformational characteristics of a series of substituted imidazo[1,2-a]pyridines and their antiulcer activity was investigated by examining the conformational properties of 3-cyano-2-methyl-8-(phenylmethoxy)imidazo[1,2-a]pyridine (1), using a variety of experimental and theoretical methods. the results of these studies was the identification of two distinctly different candidates, designated the "folded" and the "extended" conformation, respective ... | 1989 | 2754693 |
| uptake of gentamicin in foetal and newborn rats after a single intraperitoneal injection. | the aim of the present investigation has been to examine 1) the placental passage and uptake of gentamicin in foetal rat tissues and 2) uptake and organ distribution of this drug in neonatal rats with special reference to the kidney. after a single intraperitoneal injection of tritium-labelled gentamicin to the mother, we found a rapid passage of radioactivity across the rat placenta, but no pronounced tendency to accumulation in the foetus. after administration of 10 micrograms or 100 mg gentam ... | 1989 | 2755903 |
| effects of kynurenic acid and ketamine on neonatal sleep in rats. | kynurenic acid and ketamine are, besides non-specific actions, antagonists of excitatory acidic amino acids in the rat brain. at intraperitoneal doses of 300 mg/kg (1.6 mmol/l) and 5 mg/kg (18 mmol/l), respectively, they are equipotent antagonists of n-methyl-d-aspartate receptors. we studied the acute effects of ketamine and kynurenic acid, at these doses, on sleep patterns of rats during the second and third postnatal weeks with the so called static charge sensitive mattress (scsb). both kynur ... | 1989 | 2755918 |
| the timing of bedtime and waketime decisions in free-running subjects. | twelve "free-running" temporal isolation studies were performed using healthy human subjects aged 20-81 years (4 males, 8 females). circadian rhythms were measured in subjective alertness (using a visual analogue scale technique) and rectal temperature. in all 12 subjects best fitting rhythm period were found to be shorter for rectal temperature (mean = 24.3 hours) than for subjective alertness (mean = 24.8 hours). both rhythms were predictive of bedtime and waketime decisions. bedtime decisions ... | 1989 | 2756079 |
| protective effect of flavonoids on drug-induced hepatotoxicity in vitro. | primary cell cultures of neonatal hepatocytes were used to examine the protective effect of flavonoids in the presence of hepatotoxins. catechin (cat) and silybin (sil) protected the hepatocytes against cell injury produced by erythromycin estolate (ee), amitriptyline (at), nortriptyline (nt), and tert-butylhydroperoxide (tbooh). leakage of lactate dehydrogenase (ldh), aspartate aminotransferase (ast) and alanine aminotransferase (alt), as well as morphological parameters, were used as indices o ... | 1989 | 2756528 |
| [herpesvirus and acute peripheral facial paresis]. | | 1989 | 2756590 |
| assignment of human genes for phosphorylase kinase subunits alpha (phka) to xq12-q13 and beta (phkb) to 16q12-q13. | phosphorylase kinase (phk), the enzyme that activates glycogen phosphorylases in muscle, liver, and other tissues, is composed of four different subunits. recently isolated rabbit muscle cdnas for the larger two subunits, alpha and beta, have been used to map the location of their cognate sequences on human chromosomes. southern blot analysis of rodent x human somatic cell hybrid panels, as well as in situ chromosomal hybridization, have provided evidence of single sites for both genes. the alph ... | 1989 | 2757032 |
| time course of the anticonvulsant action of clonazepam during ontogenesis in the rat. | a time course of the protective action of clonazepam against metrazol-induced seizures was studied in 612 rats aged 7, 12, 18, 25 and 90 days. two doses of clonazepam (0.1 and/or 1 mg/kg) were used and metrazol (100 mg/kg in all but 18-day-old animals where 90 mg/kg were given) was administered s.c. from 30 min to 48 hr. not all time intervals were examined in all age groups. eight to 14 rats were used for each time interval, dose and age group. both doses exhibited a clear-cut antimetrazol acti ... | 1989 | 2757463 |
| perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats. | the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ... | 1989 | 2757582 |
| reduction of doxorubicin toxicity by methylene blue in cultured rat myocardial cells. | | 1989 | 2757653 |
| attempted passive transfer of a serum factor in chronic urticaria from human to monkey. | | 1989 | 2757947 |
| age changes in the crystallinity of bone mineral and in the disorder of its crystal. | the crystallinity of bone mineral and disorder of the crystal at different ages were measured by the x-ray diffraction method of ruland. measurements were made on femoral mid-diaphyses of wistar rat from 2 weeks to 1 year of age. for a given animal age, the crystallinity of bone mineral increases with age, while the overall disorder of the crystal does not vary within experimental accuracy. the increase in the crystallinity with age is attributed to an increase in crystallite size, a decrease in ... | 1989 | 2758062 |
| characterization of ion movements and their relationship to muscarinic receptor binding and excitation-contraction coupling in guinea pig ileal longitudinal smooth muscle. | the relationship between ion movements (sodium uptake and potassium release) and agonist-induced contractile responses or muscarinic receptor binding was investigated in the guinea pig ileal longitudinal muscle (gplm). sodium uptake and potassium release were agonist-dependent, concentration-dependent, and stereoselective, with the following rank order of maximum ion movement: muscarinic agonists greater than histamine greater than substance p = serotonin. potassium depolarization did not initia ... | 1989 | 2758375 |
| characterization of ion movements and their relationship to muscarinic receptor binding and excitation-contraction coupling in guinea pig ileal longitudinal smooth muscle. | the relationship between ion movements (sodium uptake and potassium release) and agonist-induced contractile responses or muscarinic receptor binding was investigated in the guinea pig ileal longitudinal muscle (gplm). sodium uptake and potassium release were agonist-dependent, concentration-dependent, and stereoselective, with the following rank order of maximum ion movement: muscarinic agonists greater than histamine greater than substance p = serotonin. potassium depolarization did not initia ... | 1989 | 2758375 |
| biological and immunological properties of sugi basic protein-pullulan conjugate. i. suppressive effect on ige antibody production and on ige-mediated reactions. | sugi basic protein (sbp), a major allergen of japanese cedar pollen, conjugated to pullulan acquires suppressive activities for ige, but not for igg antibody production, and for ige-mediated pk reactions in a sbp-specific manner. when either normal or ige anti-sbp antibody-producing mice were treated with the conjugate, ige anti-sbp antibody levels scarcely increased even after immunization with sbp + alum. this suppression was shown to be sbp specific and ige selective. the ability of sbp-pullu ... | 1989 | 2759711 |
| denervation of primate extraocular muscle. a unique pattern of structural alterations. | extraocular muscles differ from most other skeletal muscles in terms of constituent fiber types and innervation pattern. the rules that govern fiber responses to various experimental interventions for most skeletal muscles, therefore, may not strictly apply to the extraocular muscles. in this study, denervation of the extraocular muscles of cynomolgous monkeys, macaca fascicularis, was accomplished by intracranial transection of the oculomotor nerve. survival times of 3-167 days were allowed, an ... | 1989 | 2759804 |
| protection against infection with vibrio cholerae by passive transfer of monoclonal antibodies to outer membrane antigens. | ten monoclonal antibodies (mabs), produced by hybridomas obtained through the cell fusion of mouse myeloma cells and spleen cells from mice immunized with outer membrane antigens of vibrio cholerae, were mixed with suspensions of v. cholerae and administered orally to infant rabbits. six of these mabs, each one recognizing a different outer membrane antigen, did not confer protection from fatal v. cholerae infection. four mabs that reacted with the 18-kda antigen, also called the cholera protect ... | 1989 | 2760481 |
| clinical concentrations of volatile anesthetics reduce depolarization-evoked release of [3h]norepinephrine, but not [3h]acetylcholine, from rat cerebral cortex. | we compared the potencies of halothane, enflurane, and methoxyflurane in producing unconsciousness in vivo and in inhibiting the release of [3h]norepinephrine and [3h]acetylcholine in vitro. rats were anesthetized with various concentrations of each anesthetic, and responsiveness was determined by a hemostat tail pinch. slices of cerebral cortex were equilibrated with similar concentrations of each agent in vitro, and potassium-evoked release of [3h]norepinephrine and [3h]acetylcholine was deter ... | 1989 | 2760627 |
| relationship between concentration and anticonvulsant effect of phenytoin against electroshock-induced seizures in rats: comparison of sampling sites for concentration determinations. | the purpose of this investigation was to determine the optimum sampling site for phenytoin concentration measurements in the context of pharmacodynamic studies of the anticonvulsant effect of phenytoin. determination of drug concentrations in the serum, serum water, brain, and cerebrospinal fluid (csf) of rats as a function of time after iv injection of a 6-mg/kg dose revealed a significant disequilibrium between brain and serum water for 15 min and between csf and serum water for 5 min after in ... | 1989 | 2760817 |
| 2-bromo- and 16-bromo-estrogens and related compounds: potential inhibitors for both estradiol 2- and 16 alpha-hydroxylases in rat liver microsomes. | kinetic studies of inhibition of estradiol 2- and 16 alpha-hydroxylase activities in male rat liver microsomes with 2-bromoestrogens, 4-bromo-estrone (4-bre1), 16 alpha- and 16 beta-bromoestrones and 16 beta-acetylthioestrone (16-acse1) were carried out. 2-bromoestrogens and 4-bre1 nonspecifically blocked the two enzyme activities in a competitive manner, and 2-bromo-estradiol (2-bre2) was the most potent inhibitor for the two hydroxylases among the 2- and 4-bromo steroids. kinetic data, the app ... | 1989 | 2761259 |
| tamoxifen decreases the estradiol induced progesterone receptors by interfering with nuclear estrogen receptor accumulation. | the retention time of the estrogen receptor in the nucleus of target cells after antiestrogen treatment has been shown to be longer than after estradiol. this paper describes the accumulation of nuclear estrogen receptors and the obtention of estrogenic responses (i.e. synthesis of cytosolic progesterone receptors and dna) in the rat uterus after tamoxifen treatment in the presence or absence of estradiol. one-week ovariectomized adult rats were implanted with a silicone elastomer capsule contai ... | 1989 | 2761261 |
| influence of alanylglutamine infusion on gastrointestinal glutamine and alanine metabolism in anesthetized dogs. | in this study we administered an infusion of alanylglutamine (10 mumol/kg.min) for 60 minutes to postoperative anaesthetized dogs with catheters placed in the portal and hepatic veins. arterial plasma levels of alanylglutamine were 83 +/- 27 mumol/l 20 minutes after the onset of the infusion and remained constant throughout the infusion period. plasma levels of glutamine and alanine approximately doubled compared with preinfusion levels, indicating a release of these amino acids from the dipepti ... | 1989 | 2761423 |
| spectroscopic imaging by dephasing amplitude changing (sidac). | a technique for spectroscopic imaging based on a new concept of dual encoding (i.e., both phase and frequency encoding) of position along the direction of the read-out gradient is proposed. the phase encoding is produced by changing the amplitude of the dephasing gradient as in conventional phase encoding. high-resolution spectroscopic images of a normal human thigh obtained by a 2.0 tesla whole body mr imaging system are presented. | 1989 | 2762582 |
| [infection caused by paracoccidioides brasiliensis in people living in cocle and veraguas, republic of panamá]. | intradermal application of yeast phase paracoccidioidin antigen obtained by sonication and lysis from the fungus to 111 residents of veraguas province (68 men and 43 women) and 130 cocle province residents (57 men and 73 women) elicited a positive reaction in 9.01% (1/111) and 26.15% (34/30) of the veraguas and cocle residents respectively. positive reactions ranged between papules of 5 and 27 mm in diameter, the majority being between 11 and 20 mm. infection was more frequent in men (1:1.47 for ... | 1989 | 2762594 |
| experimental atherosclerosis and oxygen free radicals. | oxygen free radicals are known to produce cellular injury by peroxidation of phospholipids in the cell membrane. these free radicals might damage the endothelial cell and thus set the stage for atherosclerosis. the authors studied the effect of high-cholesterol diets on the genesis of atherosclerosis and lipid peroxidation products, malondialdehyde (mda) in rabbits. the animals were divided into four groups each comprising 5 rabbits, on the basis of their diets. group i, control diet; group ii, ... | 1989 | 2764311 |
| ecology of vibrio cholerae non-o1 and salmonella spp. and role of zooplankton in their seasonal distribution in fukuyama coastal waters, japan. | seasonal variation of human pathogens such as vibrio cholerae non-01 and salmonella spp. in fukuyama coastal waters and the role of zooplankton in their distribution were studies for a period of 1 year. comparison of two established methods, viz., the elevated temperature method and the two-step enrichment method of enumerating v. cholerae, showed that the former is superior in the recoveries of v. cholerae non-01. isolation of this pathogen on a wider range of salinities (0.4 to 32.5%) revealed ... | 1989 | 2764569 |
| effects of co-administration of di(2-ethylhexyl)phthalate and testosterone on several parameters in the testis and pharmacokinetics of its mono-de-esterified metabolite. | the administration of 1 g/kg di(2-ethylhexyl)phthalate (dehp) or 5 mg/kg testosterone for 1 week did not affect the testicular and prostatic gland weights in rats. however, co-administration of dehp and testosterone induced severe testicular atrophy accompanied by a decrease of zinc concentration in the testis and reduction of the activity of testicular specific lactate dehydrogenase isozyme. these changes were similar to the results of high dose administration of dehp alone. values of biologica ... | 1989 | 2764717 |
| effects of antibodies against chicken riboflavin carrier protein on fetal hepatic cell ultrastructure. | day 12 pregnant mice administered antibodies to crcp exhibited riboflavin deprival, which resulted in progressive alteration in the fetal hepatic cell structure, which eventually led to fetal wastage and termination of pregnancy. these changes were evident as early as 1 h following antiserum treatment. three hours following treatment, other degenerative changes such as disorganization of glycogen particles and endoplasmic reticulum and degranulation of mitochondria were observed. nuclear pycnosi ... | 1989 | 2765133 |
| [clinical manifestations of infection by human immunodeficiency virus in children]. | hiv infection in children presents a wide spectrum of clinical manifestations, from asymptomatic to opportunistic infections, neurologic deterioration and malignancy. this retrospective study describes the characteristics of 30 hiv infected children, attended at the hospital infantil de méxico. six patients were asymptomatic and 24 symptomatic. twenty four patients presented a secondary infection, 66% opportunistic infections, compared against the 100% who presented around 3 serious bacterial in ... | 1989 | 2765158 |
| [prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity]. | it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ... | 1989 | 2765581 |
| hamster circadian rhythms are phase-shifted by electrical stimulation of the geniculo-hypothalamic tract. | the suprachiasmatic nuclei (scn) contain the major pacemaker for mammalian circadian rhythms. the scn receive photic input both directly, via the retinohypothalamic tract (rht), and indirectly, via the geniculohypothalamic tract (ght), which originates in cells in the intergeniculate leaflet (igl) and anterior portions of the ventral lateral geniculate nucleus (vlgn). we tested whether electrical stimulation of the ght would induce phase shifts in wheel-running activity rhythms of syrian hamster ... | 1989 | 2765900 |
| factors affecting the colonization of isolated rabbit intestinal epithelial cells by vibrio cholerae 01 in vitro. | the adhesive capability of vibrio cholerae 01 strains to isolated rabbit intestinal epithelial cells was maximally expressed when the bacteria were grown in synthetic broth and was enhanced by the presence of ca2+ in the growth media. n-acetyl-d-glucosamine could inhibit the adhesion of the bacteria to rabbit intestinal epithelial cells as could lipopolysaccharide o-antigen from vibrio cholerae 01 and lectin from triticum vulgaris. since the lipopolysaccharide is known to contain n-acetyl-d-gluc ... | 1989 | 2766118 |
| purification and partial characterization of a cytotonic enterotoxin produced by aeromonas hydrophila. | this report describes the purification and partial characterization of a cytotonic enterotoxin produced by a human diarrheal isolate (ssu) of aeromonas hydrophila. the extracellular enterotoxin was purified by (nh4)2so4 precipitation, hydrophobic column chromatography, and chromatofocusing. the highly purified enterotoxin exhibited a molecular mass of 44 kda and an isoelectric point in the range of 4.3 - 5.5 as determined by chromatofocusing. western blot analysis using aeromonas anti-enterotoxi ... | 1989 | 2766121 |
| treatment of childhood bacterial meningitis with ceftriaxone once daily: open, prospective, randomized, comparative study of short-course versus standard-length therapy. | fifty-two children were included in this study to evaluate and compare short- versus standard-length ceftriaxone therapy for bacterial meningitis. the duration of the short-course regimens was 4, 6 and 7 days for neisseria meningitidis, hemophilus influenzae and streptococcus pneumoniae, respectively. the standard-length regimens were twice as long. on the basis of a computer-generated randomization list, 26 children were assigned either to the short- or to the standard-treatment regimen. ceftri ... | 1989 | 2766869 |
| transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene. | two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ... | 1989 | 2766934 |
| exclusion of clinically atypical or microbiologically mixed diarrhoeal episodes from outcome events in a field trial of oral cholera vaccines. | we investigated whether alternative clinical and microbiological criteria for outcome events affected estimates of vaccine efficacy in a randomized, double-blind field trial of b subunit-killed whole cell (bs-wc) and killed whole cell-only (wc) oral cholera vaccines among 62,285 rural bangladeshi participants. at one year of follow-up estimates of vaccine protective efficacy (pe = 60%, p less than 0.0001 for bs-wc; pe = 54%, p less than 0.0001 for wc) against all treated diarrhoeal episodes asso ... | 1989 | 2767860 |
| detection of trypsin- and chymotrypsin-like proteases using p-nitroanilide substrates after sodium dodecyl sulphate polyacrylamide gel electrophoresis. | specific chromogenic p-nitroanilide substrates have proved useful for localizing proteolytic enzymes, such as trypsin, chymotrypsin and elastase after separation by agarose gel electrophoresis and when immobilized on nitrocellulose. this procedure was further developed for use with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). after sds-page, proteins were transferred electrophoretically to a nitrocellulose membrane. the membrane was incubated for 10-60 min with bz-ile-g ... | 1989 | 2768384 |
| vibrio cholerae non-serogroup o1 cystitis. | we report a case of a patient who developed cystitis caused by non-serogroup o1 vibrio cholerae after swimming in the chesapeake bay. treatment was empirical, with complete symptomatic resolution. genitourinary tract infections by vibrio spp. are uncommon but should be considered when cystitis occurs after saltwater exposure in appropriate geographic regions. | 1989 | 2768474 |
| isolation and morphological characterization of el tor cholera phages. | five bacteriophages have been isolated for phage typing of vibrio cholerae biotype el tor. the morphology of these phages has been studied by electron microscopy. | 1989 | 2769238 |
| studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro. | we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ... | 1989 | 2769548 |
| studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro. | we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ... | 1989 | 2769548 |
| [cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control]. | fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ... | 1989 | 2772364 |
| infected false aneurysms of the femoral artery in intravenous drug addicts. | the evaluation and treatment of 60 intravenous drug addicts with infected false aneurysms of the femoral artery seen at henry ford hospital (detroit) from 1977 to 1988 were retrospectively reviewed. the most common presenting signs and symptoms were groin swelling and/or mass in 56 (93%) of the patients and pain and/or tenderness in 48 (80%) of the patients. digital subtraction angiography and standard arteriography showed sensitivities of 92% and 96%, respectively, in the detection of aneurysms ... | 1989 | 2772464 |
| pseudomonas cepacia typing systems: collaborative study to assess their potential in epidemiologic investigations. | to determine the utility of available pseudomonas cepacia typing systems for confirming the relatedness of isolates, we applied these methods to isolates associated with previously investigated nosocomial outbreaks. we compared chromosome analysis, serologic reactions, biochemical tests, bacteriocin production and susceptibility, and antimicrobial susceptibility in their ability to determine outbreak relatedness. chromosome analysis, serologic reactions, and biochemical tests were each demonstra ... | 1989 | 2772466 |
| twenty-year comparison of the human allograft and porcine xenograft. | this study compares a retrospective consecutive series of human allografts and concurrent porcine xenografts implanted over a 10-year interval. there were 571 allograft valves and 1,351 xenograft valves implanted in the aortic and mitral position with mean follow-up of 12.8 years for the allografts and 6.2 years for the xenografts. this study compares the incidence of structural deterioration over long-term follow-up. we found that there was no significant difference between four manufacturers o ... | 1989 | 2774760 |
| cholera in louisiana. widening spectrum of seafood vehicles. | the largest cholera outbreak in the united states in over a century occurred in louisiana from august through october 1986. eighteen persons in 12 family clusters had stool culture or serologic evidence of infection with toxigenic vibrio cholerae 0-group 1. thirteen of these persons had severe diarrhea, and 4 required intensive care unit treatment. although all 18 survived, 1 96-year-old woman with suspected cholera died shortly after hospital admission. a case-control study showed that case-pat ... | 1989 | 2774784 |
| primary structure of a novel 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits)-binding membrane protein highly expressed in torpedo californica electroplax. | polyclonal rabbit antibodies were raised against 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits), an inhibitor of a variety of anion transport proteins. these antibodies specifically recognize sits-reacted erythrocyte band 3 in immunoprecipitations and western blots. in western blots of sits-reacted membrane proteins derived from vesicles of the electric organ of torpedo californica (known to express a sits-sensitive cl- channel) the antibodies recognized two major species of ap ... | 1989 | 2775201 |
| metabolism of platelet-activating factor (paf; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-paf (1-o-alkyl-2-lyso-sn-glycero-3-phosphocholine) by cultured rat kupffer cells. | the metabolism of platelet-activating factor (paf; identified as agepc: 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-paf (lyso-gepc: 1-o-alkyl-2-lyso-sn-glycero-3-phosphocholine) was investigated in cultured rat kupffer cells. the rat kupffer cells accumulated [3h]agepc and deacetylated this compound to the corresponding [3h]lyso-gepc, which was the major metabolic product of [3h]agepc. [3h]lyso-gepc was distributed primarily in the supernatant fraction of incubated cells throughout ... | 1989 | 2775221 |
| sialidase from different sources compared for electrophoretically separating serum alkaline phosphatase fractions from liver and bone. | we compared sialidase (neuraminidase; ec 3.2.1.18) from vibrio cholerae, clostridium perfringens, and arthrobacter ureafaciens, seeking to improve the electrophoretic separation of the liver and bone isoenzymes of alkaline phosphatase (ec 3.1.3.1) on cellulose acetate membranes. resolution is decisively determined by the type and activity of sialidase used in the preincubation of serum sample. sialidase from arthrobacter ureafaciens is not suited for this method. for optimal separation of the tw ... | 1989 | 2776324 |
| in vitro determination of phagocytosis and intracellular killing of brucella melitensis by goat's polymorphonuclear phagocytes. | one hundred and forty samples of goats serum taken from animals suspected of having brucellosis, have been studied by trying to assess the phagocytosis in vitro of brucella melitensis rev-1 by polymorphonuclear neutrophil phagocytes opsonized with antibrucella serum. the serum agglutination test and the coombs antiglobulin test were carried out with the serum samples. the opsonization ingestion, intracellular killing and germ killing power of the serum were also assessed. the results demonstrate ... | 1989 | 2776406 |
| comparison of trimethadione and antipyrine as indicators of oxidative drug metabolizing capacity in man. | ten healthy male volunteers were given trimethadione (tmo) 4 mg/kg and antipyrine (ap) 500 mg alone or concomitantly to determine whether the metabolism of the drugs was mediated by the same or closely related forms of cytochrome p-450. whether administered alone or together the clearance (cl) and half-life (t 1/2) of tmo and ap were the same, and there was a good correlation between the cl and t 1/2 of tmo and ap (alone r = 0.755 and 0.623, respectively; coadministered r = 0.771 and 0.503, resp ... | 1989 | 2776822 |
| comparative pharmacokinetics of zidovudine (azt) and its metabolite (g.azt) in healthy subjects and hiv seropositive patients. | | 1989 | 2776824 |
| suppression of antibody responses to the acetylcholine receptor by natural antibodies. | some relationships between "natural" physiological autoreactivity and antibody responses against the acetylcholine receptor (achr) were investigated. collections of unselected monoclonal antibodies were derived from nonmanipulated newborn mice of susceptible (balb.b) and resistant (balb/c) mouse strains, and screened for reactivity with heterologous achr. five in 200 balb/c but none in 200 balb.b clones were found reactive. four of these anti-achr antibodies were analyzed for connectivity to nin ... | 1989 | 2776827 |
| comparison of selective and complete inhibitors of enkephalin-degrading enzymes on morphine withdrawal syndrome. | we investigated the effects of thiorphan, a selective inhibitor of endopeptidase 24.11 'enkephalinase', kelatorphan ((r)-3-(n-hydroxy-carboxamido-2-benzylpropanoyl)-l-alanine), and rb 38 a ((r)-3-(n-hydroxycarboxamido-2-benzylpropanoyl)-l-phenylalanine) two almost complete inhibitors of enkephalin metabolism, on the naloxone-precipitated morphine withdrawal syndrome in rats. inhibitors administered intracerebroventricularly reduced several symptoms of the withdrawal syndrome. jumping, chewing an ... | 1989 | 2776828 |
| neutralisation of the new cholera toxin by antiserum against crude enterotoxin of cholera toxin gene-positive vibrio cholerae 01 in rabbit ileal loop model. | the enterotoxicity of the new cholera toxin (nct) prepared from cholera toxin gene-negative (ct-) v. cholerae 01 strains isolated from human diarrhoeal and environmental sources was assayed in rabbit ileal loops and the toxin unit was calculated to be 24 micrograms of protein. the enterotoxicity of the nct preparations were completely neutralised by the antiserum raised against the enterotoxin preparation from the ct+ v. cholerae 01 strain 569b at 1 in 16 dilution in ileal loops. the antiserum c ... | 1989 | 2777342 |
| a survey of the human t-cell lymphotropic virus type i (htlv-i) in south-western colombia. | to evaluate altitude of birthplace and residence as factors associated with geographic clustering of htlv-i infection in colombia, we sampled a total of 670 current residents of the south pacific coastal lowland and of upland regions (cali and environs) of the valle and cauca provinces, located at an altitude of 3,100 ft. among the 255 lowland study subjects, 4.3% had antibody against htlv-i, compared to 0.9% of the 415 upland study subjects. a hypothesis emerging from this study is that the low ... | 1989 | 2777408 |
| an actin-depolymerizing protein in embryonic chicken skeletal muscle: purification and characterization. | in embryonic skeletal muscle, a large amount of non-polymerized actin exists in the cytoplasm (shimizu and obinata [1986] j. biochem. 99, 751-759). a 19-kda protein (called 19k protein) which binds to g-actin was purified by sequential chromatography on dnase i-agarose, hydroxylapatite, sp-sephadex, and sephadex g-75, from the sarcoplasmic fraction of embryonic chicken skeletal muscle. this protein decreased the extent of actin polymerization at a steady state and increased the monomeric actin i ... | 1989 | 2777748 |
| [total anomalous pulmonary venous connection in adult: report of a case]. | total anomalous pulmonary venous connection (tapvc) is a cardiac disorder that seldom permits survival into adulthood without surgical correction in infancy. we reported a successful total correction in a 47-year-old man with tapvc. cardiovascular angiography demonstrated the features of darling i a type tapvc. cardiac catheterization showed qp/qs: 5.8, pp/ps: 0.31, rp/rs: 0.06 and mild pulmonary hypertension. on operation, the posterior wall of left atrium anastomosed to the common pulmonary ve ... | 1989 | 2779053 |
| hemolytic vibrio cholerae o1 that is sensitive to mukerjee's cholera phage iv and the phage produced by the hemolytic vibrio lysogenized with the infection of mukerjee's cholera phage iv. | strains of hemolytic vibrio cholerae o1 (el tor vibrio) which are sensitive to mukerjee's cholera phage group iv were isolated from cholera patients in north-east thailand in 1986. plaques of the phage on these hemolytic v. cholerae o1 were usually translucent but almost transparent on some strains, just like the plaques on non-hemolytic v. cholerae o1 (classical vibrio). these hemolytic v. cholerae o1 were lysogenized with the infection of cholera phage iv, and the lysogenized strains produced ... | 1989 | 2779469 |
| sugar composition of the polysaccharide portion of lipopolysaccharides isolated from non-o1 vibrio cholerae o2 to o41, o44, and o68. | the sugar composition of the polysaccharide portion of lipopolysaccharides (lps) was determined for 42 serovars of non-o1 vibrio cholerae, i.e., from serogroups o2 to o41, o44, and o68. on the basis of their compositional sugar pattern, they were classified into 24 chemotypes. 2-keto-3-deoxyoctonate (kdo) was totally undetectable by the conventional color test (weissbach's reaction) under the conventional hydrolysis conditions. instead, a kind of kdo-like substance, which was positive in the rea ... | 1989 | 2779472 |
| purification of cross-reacting protein antigen shared by yersinia enterocolitica and other gram-negative bacteria with monoclonal antibody. | a monoclonal antibody against the yersinia enterocolitica 60-kilodalton (kda) antigen, designated cross-reacting protein antigen (crpa), was obtained by cell fusion. the crpa common to gram-negative bacteria was purified from y. enterocolitica by the affinity chromatography with the monoclonal antibody (igg1) thus obtained. the purified crpa showed a single band of 60 kda in sds-polyacrylamide gel electrophoresis (sds-page), and reacted with rabbit antisera against y. enterocolitica, vibrio chol ... | 1989 | 2779474 |
| evolution of androgen-regulated ornithine decarboxylase expression in mouse kidney. | the analysis of naturally occurring variations in hormone-regulated gene expression generates insights into the mechanisms governing evolutionary changes in hormone response. in the mouse (genus mus), kidney ornithine decarboxylase (odc) expression, which is regulated by androgens, has been modified during evolution. this has resulted in intra- and interspecies variations that have accumulated over a 10-15 million year time period. we have examined odc expression and its response to androgens in ... | 1989 | 2779581 |
| rat gene 33: analysis of its structure, messenger rna and basal promoter activity. | several overlapping cdna and genomic dna clones corresponding to mrna g33 and gene 33, respectively, were isolated and characterized. the mrna g33 molecule is 2971 nt in length, exclusive of a poly(a+) tail, and encodes a putative 459 amino acid protein (49,946 daltons). the 13.2 kbp gene consists of four exons, three introns, and has two initiation sites located 27 and 30 bp downstream from a tata box. transfection of h4iie cells with a fusion gene 33 (-1900 to +32)/luciferase construct (psl330 ... | 1989 | 2780291 |
| reasons for the ineffectiveness of eglin c to ameliorate endotoxin shock in sheep. | | 1989 | 2780742 |