| incidence of strains producing extended spectrum beta-lactamases in argentina. | the incidence of strains producing transferable beta-lactamases capable of hydrolyzing third generation cephalosporins or aminothiazole-oximino substituted monobactams in five buenos aires hospitals during a four month period was studied. these enzymes were categorized by 1) mic greater than or equal to 1 mg/l for third generation cephalosporins; 2) mic less than 0.06 mg/l for third generation cephalosporins combined with clavulanic acid or sulbactam; 3) sensitivity to imipenem or cephamycins (e ... | 1989 | 2613338 |
| in-vitro activity of cefetamet (ro 15-8074) compared with other oral agents. | the bacteriostatic and bactericidal activities of cefetamet (ro 15-8074) were compared with those of cefadroxil, cefaclor, ampicillin, doxycycline, chloramphenicol and erythromycin against a total of 400 bacterial strains isolated from nosocomial respiratory tract and auditory canal infections. the broth macrodilution method was used to determine antimicrobial susceptibility. none of the compounds was effective against pseudomonas aeruginosa and methicillin-resistant staphylococcus aureus strain ... | 1989 | 2613603 |
| [treatment of mediastinal infection following cardiac surgery by continuous closed irrigation with povidone-iodine in early infancy]. | two patients aged 2 months and 9 days with postoperative mediastinal infection were treated by continuous closed irrigation with 0.5% povidone-iodine solution. satisfactory eradication was achieved in both two patients. although serum iodine level was elevated as high as 7,610 micrograms/dl in the neonatal patient, no disturbances of the thyroid function and the acid-base balance were seen. the usefulness of continuous closed irrigation in the neonatal or infantile period was suggested. | 1989 | 2614126 |
| glutathione transferase in bacteria: subunit composition and antigenic characterization. | the presence of glutathione transferase (gst; ec 2.5.1.18) in escherichia coli atcc 25922, e. coli atcc 25422, proteus vulgaris atcc 8427, pseudomonas aeruginosa atcc 27853, klebsiella oxytoca cip 666, k. oxytoca af 101, enterobacter cloacae cip 6085, serratia marcescens cip 6755, and proteus mirabilis af 2924 was investigated. using 1-chloro-2,4-dinitrobenzene as substrate, gst activity was found in the glutathione-(gsh-)affinity-purified fraction of all strains tested. sds-page analysis of gsh ... | 1989 | 2614380 |
| upper small intestinal microflora in diarrhea and malnutrition in nigerian children. | the upper small intestinal microflora was determined quantitatively and qualitatively in a group of well-nourished diarrhea-free nigerian children and compared with those of well-nourished children with acute diarrhea and malnourished children with or without diarrhea. intestinal aspirate was collected by intubation after a 6-h fast. well-nourished children without diarrhea had flora consisting predominantly of gram-positive cocci. total bacterial counts were less than 10(5) organisms/ml; 18.2% ... | 1989 | 2614617 |
| [therapy of chronic recurrent respiratory tract infections]. | in 1987, the most frequently identified pathogens in chronic respiratory tract infections in our clinic were haemophilus influenzae, pseudomonas aeruginosa, branhamella catarrhalis, streptococcus pneumoniae, staphylococcus aureus and klebsiella pneumoniae. recurrent infection is a common phenomenon in patients with chronic respiratory tract infections, including chronic bronchitis, chronic bronchiolitis and bronchiectasis. h. influenzae is the most common pathogen in such patients. macrolides, t ... | 1989 | 2615085 |
| production of a unique cytotoxin by klebsiella oxytoca. | certain strains of klebsiella oxytoca isolated from patients with hemorrhagic enterocolitis produced a unique cytotoxin. the cytotoxin induced rounding of tissue culture cells, such as hep-2, vero, cho and hela cells. the induced morphologic changes were indistinguishable between cell types. seventy to 80% of the rounded cells died in 48 h incubation. the cytotoxin was purified 1000-fold from culture supernatant by sephadex g-25 and bio-gel p-2 gel filtration followed by reversed-phase high-perf ... | 1989 | 2615635 |
| [in vitro susceptibility to a new antimicrobial agent (imipenem) of pathogens isolated from inpatients at various centers]. | imipenem is a beta lactam antibiotic, a highly potent new carbapenem with broad antibacterial spectrum. to test the "in vitro" efficacy of this antimicrobial agent in pathogens more frequent in several medical centers in brazil, susceptibility testing with 10 mcg imipenem disks and, or corresponding mic were carried out with 1231 recent isolates of 41 different bacterial species, obtained mainly from hospitalized patients in 5 different medical centers of the cities of s. paulo, rio de janeiro a ... | 1989 | 2617012 |
| antimicrobial susceptibility of selected bacterial enteropathogens in latin america and worldwide. | we conducted an in vitro susceptibility study of bacterial pathogens to various antimicrobials. strains of shigella, salmonella, escherichia coli, and klebsiella pneumoniae collected in the 1960s, 1970s, and 1980s at the hospital infantil de mexico federico gomez were tested against ampicillin, chloramphenicol, tetracycline, trimethoprim-sulfamethoxazole, amikacin, gentamicin, and furazolidone. over the 3-decade period, the resistance of enteropathogens to furazolidone showed the least overall i ... | 1989 | 2617180 |
| pulmonary diseases in patients infected with the human immunodeficiency virus in zimbabwe, central africa. | during the 11 month period up to 30 september 1987, 37 patients (26 male, 11 female, mean age 27 years) with respiratory symptoms who were human immunodeficiency virus (hiv) positive, were studied prospectively on 40 occasions to determine the cause of any pulmonary complications. hiv was heterosexually transmitted. predominant symptoms were cough (89%), fever (89%), weight loss (83%), and dyspnoea (60%). transnasal fibre-optic bronchoscopy (with bronchoalveolar lavage, bronchial brushings and t ... | 1989 | 2617633 |
| [normal microflora of the pharyngeal mucosa]. | aerobic microflora of the throat mucosa was studied in 518 healthy persons aged 1 to 50 years. on the basis of the study results, criteria for estimating microbiocenoses of the upper respiratory tracts were defined. it was shown that the throat symbiotic flora included three groups of microorganisms playing different roles in the development of microbiocenosis. the indigenous group consisted of representatives of streptococcus and neisseria and was characterized by permanent (90-100 per cent) an ... | 1989 | 2619405 |
| new diterpenoid antibiotics, spirocardins a and b. | new antibiotics spirocardins a and b were isolated from the culture broth of an actinomycete isolated from a soil sample collected near lake hibara, fukushima prefecture, japan. the producing strain was classified as nocardia sp. sank 64282. the antibiotics were isolated from the culture filtrate by solvent extraction and purified further by silica gel and preparative reverse phase column chromatography. they were primarily active against gram-positive bacteria including methicillin-resistant st ... | 1989 | 2621157 |
| in vitro and in vivo antibacterial activity of kt3777, a new orally active carbacephem. | kt3777 is a novel carbacephem antibiotic structurally identical to cefaclor (ccl), except that the sulfur atom of position 1 of the cephem nucleus has been replaced by carbon. kt3777 was investigated for in vitro and in vivo antibacterial activities in comparison with ccl, cephalexin (cex) and amoxicillin. the mic50 of kt3777 ranged from 0.2 to 3.13 micrograms/ml for clinical isolates of staphylococcus aureus, streptococci, escherichia coli, klebsiella pneumoniae, proteus mirabilis, haemophilus ... | 1989 | 2621166 |
| monoclonal antibodies reacting with the exopolysaccharide xanthan from xanthomonas campestris. | we have prepared murine hybridomas secreting monoclonal antibodies against the exopopolysaccharide xanthan from xanthomonas campestris pv. campestris 646 after fusing nso myeloma cells and spleen cells from balb/c mice immunized with xanthan. four hybridomas, secreting antibodies designated a6 (igm kappa), b3 (igm kappa), d1 (igm kappa), and d3 (igg2a kappa), were selected for further studies. all antibodies reacted with a range of different xanthans. competition studies using variants of the ex ... | 1989 | 2621443 |
| [determination of the mic of cefotetan against freshly isolated gram-negative bacilli]. | antimicrobial activities (mics) of cefotetan (ctt) against 575 strains of 16 spp. of gram-negative bacilli isolated in 1988 were determined to investigate distribution of mics in comparison with those of cefmetazole (cmz), cefoxitin (cfx), latamoxef (lmox) and cefazolin (cez). the change in frequencies of incidence of cephem-resistant strains in the latter half of the 1980 was also investigated. distribution of mic of ctt varied with test strains. no or very few mics were at or higher than 12.5 ... | 1989 | 2621779 |
| [clinical laboratory approach to estimating the effective administration dose of imipenem/cilastatin. evaluation of the disk susceptibility test and its interpretation system]. | in vitro activities of imipenem/cilastatin (ipm/cs) against 413 clinical isolates were studied through the evaluation of mics and the results of disk susceptibility tests. the mics were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. the mic80s of imipenem (ipm) against staphylococcus aureus and staphylococcus epidermidis were 25 and 1.56 micrograms/ml, respectively, showing a bimodal mic distribution. however, the distribution of mics against other bacteria studi ... | 1989 | 2621780 |
| [study on cefotaxime in respiratory surgery: transfer to lung tissue and kinetics in serum]. | cefotaxime (ctx) was intravenously administered in a dose of 1 g to patients just prior to lung surgery. lung tissue specimens were collected at 1, 2 and 3 hours after the ctx administration, and the concentration of ctx in each specimen was determined. at the same time, the concentration of ctx in the serum was also measured. the results are summarized below. 1. determination of the ctx concentration in the lung tissue using bioassay showed values of 3.78 +/- 1.93 micrograms/g at 1 hour after c ... | 1989 | 2621782 |
| [the sensitivity to antibiotics and chloramine b of opportunistic microorganisms isolated from patients]. | strains of staphylococci, klebsiella, proteins, blue pus bacillus and enterobacteria resistant not only to antibiotics but also to chloramine, a disinfectant, were isolated from the test material samples obtained from patients with different purulent-inflammatory processes. the following strains are revealed among the test microorganisms: sensitive simultaneously to antibiotics and chloramine; sensitive to antibiotics but resistant to chloramine; sensitive to chloramine but resistant to antibiot ... | 1989 | 2622404 |
| [bacteriologic monitoring in a surgical department with emergency services. epidemiologic analysis and study of sensitivity to antibiotic therapy]. | the two year experience with 2091 biological cultures and the related antibiograms has been reported on the basis of a prospective, computerized study. the incidence of germs coming from each fluid collected has been analyzed as well as the sensibility to various antibacterial drugs. the gram negative such as escherichia coli, enterococcus, pseudomonas, proteus, klebsiella, serratia and enterobacter resulted more frequently isolated; in addition a revival of gram positive strains (staphylococcus ... | 1989 | 2622558 |
| comparison of bacteriologic culture of blood and necropsy specimens for determining the cause of foal septicemia: 47 cases (1978-1987) | diagnosis of bacterial septicemia was confirmed by results of bacteriologic culture of antemortem blood samples and/or necropsy specimens obtained from 47 foals up to 8 days old. gram-negative bacteria were isolated from all 47 foals, and mixed infections with more than one organism were involved in 26 (55%). escherichia coli, actinobacillus spp, and klebsiella pneumoniae were the most frequent isolates, infecting 55, 34, and 23% of foals, respectively. gram-positive bacteria and anaerobic bacte ... | 1989 | 2624660 |
| biliary stones and bacteriae in bile study in 211 consecutive cases. | a retrospective study have been performed to investigate the correlation between stone formation and bacteriae in the bile of 211 consecutive biliary stone patients (133 females and 78 males, age ranged 1.3 to 87.0 years old, 196 cholecystolithiasis, 54 choledocholithiasis and 9 hepatolithiasis). they had undergone surgery since january 1980 to december 1987. bile was collected to perform bacterial culture from the gallbladder and common bile duct during operation and all stones had been classif ... | 1989 | 2626748 |
| [results of intraoperative bacteriological studies of bile from patients operated on for cholelithiasis]. | complete bacteriological examination of bile was carried out in 123 patients operated on for cholecystolithiasis. the most frequently found gram-negative bacteria were e. coli and klebsiella species. the gram-positive organisms were most frequently staphylococcus epidermidis, str. viridans and str. faecalis. anaerobes were rarely found. the effectiveness of the antibiotics used was studied. | 1989 | 2629315 |
| [epidemiologic problems of gastrointestinal infections in children 0 to 2 years of age]. | the analysis is presented of certain epidemiological problems based on a material including 774 cases of gastrointestinal infections hospitalized in two paediatric centres of the province paediatric hospital in warsaw in the years 1979/1981/1983. faeces were examined obtained from children aged 0 to 24 months treated in hospital for diarrhoea. the material was presented in tables and was subjected to statistical analysis. the absolute number of the treated infants with diarrhoea increased succes ... | 1989 | 2629324 |
| [patterns of resistance of staph aureus and gram-negative bacteria to aminoglycosides and cephalosporins]. | the sensitivity to 4 aminoglycoside antibiotics (gentamicin, tobramycin, netilmycin and amicacin) and 5 cephalosporins (cefradine, cefamandol, cefotaxime, cefoperazone and ceftriaxone) was determined in 700 bacterial strains isolated from clinical materials in the years 1986-1987. the most frequent coexistent resistance was observed to gentamicin and tobramycin in s. aureus (30%), klebsiella (30%), proteus mirabilis (28%) and enterobacter (23%). resistance to 5 cephalosporins was found in entero ... | 1989 | 2629327 |
| antimicrobial activity of dactimicin in vitro compared with that of dibekacin, netilmicin, sisomicin and micronomicin. | antimicrobial activity of dactimicin, a pseudo-disaccharide aminoglycoside antibiotic, was compared with those of dibekacin, netilmicin, sisomicin and micronomicin using clinical isolates of four gram-positive and sixteen gram-negative bacteria. dactimicin was more active than the reference amino-glycosides against serratia marcescens, especially gentamicin-resistant serratia sp., proteus vulgaris, p. rettgeri and klebsiella oxytoca, but less active against pseudomonas aeruginosa and p. mirabili ... | 1989 | 2632214 |
| oral gram-negative bacilli in bone marrow transplant patients given chlorhexidine rinses. | fifteen bone marrow transplant (bmt) patients who received three 0.12% chlorhexidine digluconate (chx) mouthrinses daily for eight weeks were monitored weekly for the occurrence of oral opportunistic gram-negative bacilli (gnb). tongue and buccal mucosa were sampled with use of culturette swabs that were streaked on plates containing selective macconkey agar. after incubation, colony-forming units were scored and putative gnb classified with use of the api 20e rapid identification system and sup ... | 1989 | 2632605 |
| gall bladder disease: an analytical report of 250 cases. | two hundred and fifty cases of biliary tract disease were studied as regards case history, physical and laboratory investigations, surgery and follow-up. it was found that females especially multipara were frequently affected; majority of cases were in 3rd to 5th decade of their life, rise in age showing decline in incidence. majority of cases (82.4%) were vegetarians and had used vegetable fats (oriental diet). most of the cases (98.8%) belonged to middle and poor class and were lean and thin. ... | 1989 | 2634700 |
| [antibacterial action of some saturated and unsaturated long-chain carboxylic acids and their bis(2-chloroethyl)aminoethyl esters in vitro]. | using the method of twofold serial dilution of substances in liquid nutrient medium with subsequent reseeding on solid nutrient medium a comparative study has been carried out of the antibacterial action in vitro of long chain saturated (c10-c18) and unsaturated (c11-c22) carboxylic acids and their bis(2-chloroethyl)aminoethyl esters against pseudomonas aeruginosa, klebsiella pneumoniae tp 16 koph, escherichia coli atcc 25922, staphylococcus aureus 101+ and proteus mirabilis 56. the compounds st ... | 1989 | 2635322 |
| a clinical evaluation of sulbactam/ampicillin in the treatment of pediatric infections. | we have treated 42 episodes of pediatric infections with sulbactam/ampicillin since 1987. included were 9 cellulitis, 9 urinary tract infections, 5 cervical lymphadenitis, 4 meningitis, 2 thoracic empyema, 2 osteomyelitis, 2 sepsis, 1 furuncle, 1 perianal abscess, 1 dental abscess, 1 peritonsillitis, 1 salmonellosis, 1 shigellosis, 1 peritonitis, 1 suppurative thyroiditis, 1 infective endocarditis. responsible pathogens were escherichia coli in 8, staphylococcus aureus in 6, hemophilus influenza ... | 1989 | 2637593 |
| urinary tract infections in children. | from january 1981 to december 1987, 346 children with urinary tract infections, proved by urine culture, were admitted to the department of pediatrics, mackay memorial hospital. the ratio of male to female was 3.0 in children below 2 years, and 0.8 in children above 2 years, of age. the urine specimens were collected from suprapubic punctures in 281 cases (81.2%). fever was the most common clinical manifestation. in children below two years old, other common symptoms and signs were body weight l ... | 1989 | 2637602 |
| spontaneous focal intestinal perforation in prematurity: report of three cases. | three premature neonates with a localized perforation of the intestine, but not associated with necrotizing enterocolitis (nec) or gastrointestinal anomaly are reported. the birth weight of these babies was around 1500 g and they exhibited striking similarities in the clinical course. before laparotomy, nec was firstly impressed. abdominal distension, refusal of feedings, poor activity and respiratory distress were the major manifestations. pneumoperitoneum was all detected before operation. the ... | 1989 | 2637614 |
| neonatal septicaemia--incidence, etiology and outcome. a 6-year analysis. | between 1983 and 1988 we observed altogether 222 cases of neonatal septicemia and/or meningitis in our department of neonatology. the incidence was 8.46 per 1,000 liveborn infants. the case fatality rate amounted to 45.9%. the most frequently isolated causative agents were escherichia coli (23.4%) followed by group b streptococci (16.7%), staphylococcus aureus (9.9%), klebsiella pneumoniae species (8.8%), serratia marcescens (7.9%), pseudomonas aeruginosa and coagulase-negative staphylococci eac ... | 1989 | 2642246 |
| bacterial infections among patients with diabetes in papua new guinea. | this study reports the morbidity that resulted from bacterial infections in melanesian patients with non-insulin-dependent diabetes who attended the port moresby general hospital, papua new guinea, between january 1, 1982 and june 30, 1984. fifty-three of 160 patients with diabetes experienced 66 episodes of infection, 48 of which required inpatient hospital treatment. the average length of stay in hospital was 37.6 days per episode of infection. of 88 patients who were newly-diagnosed as diabet ... | 1989 | 2642592 |
| three different types of catalases in klebsiella pneumoniae. | crude extracts from aerobically grown bacterium klebsiella pneumoniae contain three different types of catalases, designated kpt, kpcp, and kpa, whose activities in crude extracts are in the ratio 4.1:1:0.3. kpt resembles typical catalases: its molecular weight is 259,000, its activity is independent of ph in the range 5.5-10.5, it is stable at 52 degrees c but loses 80% of its activity when incubated at 60 degrees c for 45 min, and it has hydrophobic properties revealed by binding to phenyl-sep ... | 1989 | 2643382 |
| the roles of the nifw, nifz and nifm genes of klebsiella pneumoniae in nitrogenase biosynthesis. | active fe protein of nitrogenase was synthesised in a non-nitrogen fixing organism when escherichia coli was transformed with a plasmid encoding only two nif-specific genes, nifh and nifm of klebsiella pneumoniae. hence proteins nifh and nifm are sufficient to produce active fe protein in e. coli. k. pneumoniae strains carrying chromosomal nifw- and nifz- mutations were constructed and shown to be significant c2h2-reducing activity and to grow on n-free plates. nevertheless, derepressing culture ... | 1989 | 2643516 |
| mucoid phenotype of klebsiella pneumoniae is a plasmid-encoded virulence factor. | we have previously reported that the presence of a 180-kilobase plasmid encoding production of aerobactin was correlated with the virulence of klebsiella pneumoniae k1 and k2 isolates. this work demonstrates that a variant of a k2 strain which has lost this plasmid, pkp100, becomes avirulent. labeling of this plasmid with the mobilizable, replication-defective element pme28, used here as a mobilizable transposon, allowed the transfer of this plasmid into a plasmidless derivative. virulence was r ... | 1989 | 2643575 |
| isolation and characterization of klebsiella pneumoniae unencapsulated mutants. | klebsiella pneumoniae mutants were obtained after uv irradiation and negative selection with anticapsular serum. unencapsulation, rather than expression of a structurally altered capsule, was found in the mutants. the mutant strains showed no alterations in their outer membrane proteins and lipopolysaccharide, and a great similarity with the wild type in the properties tested (serum resistance, antimicrobial sensitivity, and lipopolysaccharide-specific bacteriophage sensitivity), with the except ... | 1989 | 2643625 |
| invasion of vero cells by salmonella species. | the invasiveness of salmonella strains for vero cells was studied by quantitative bacteriology; the technique was more sensitive than phase contrast microscopy. all of 59 salmonella strains, of 19 different serotypes, were more invasive than escherichia coli k12. three strains of shigella were as invasive as most of the salmonella strains whereas 29 strains of e. coli, two of proteus, three of klebsiella and one of serratia were much less invasive. two citrobacter strains exhibited intermediate ... | 1989 | 2643706 |
| direct and indirect pathogenicity of beta-lactamase-producing bacteria in mixed infections in children. | the recent emergence of numerous aerobic and anaerobic beta-lactamase-producing bacterial strains has been associated with an increase in the failure rate of penicillins in the therapy of infection caused by these organisms. these include respiratory tract, skin of soft tissue, female genital tract, intra-abdominal, and other miscellaneous infections. the important aerobic beta-lactamase-producing bacteria (blpb) include staphylococcus aureus, branhamella catarrhalis, haemophilus sp., neisseria ... | 1989 | 2644068 |
| characterization of a wide host range plasmid panv-6 from citrobacter diversus. | panv-6 is a 5.85 kb, streptomycin resistant, high copy number plasmid isolated from a multi drug resistant clinical isolate of c. diversus by transformation in escherichia coli c-600. the plasmid was very stable, noncurable and could not be amplified with chloramphenicol. it was non-conjugative among enterobacteriaceae hosts. plasmid was transferred by transformation to several gram negative and gram positive hosts. these included esch. coli, serratia marcescens, salmonella typhimurium, klebsiel ... | 1989 | 2644170 |
| physical and genetic map of the major nif gene cluster from azotobacter vinelandii. | determination of a 28,793-base-pair dna sequence of a region from the azotobacter vinelandii genome that includes and flanks the nitrogenase structural gene region was completed. this information was used to revise the previously proposed organization of the major nif cluster. the major nif cluster from a. vinelandii encodes 15 nif-specific genes whose products bear significant structural identity to the corresponding nif-specific gene products from klebsiella pneumoniae. these genes include nif ... | 1989 | 2644218 |
| comparative antibiotic dose-effect relations at several dosing intervals in murine pneumonitis and thigh-infection models. | animal studies that compare antibiotics have used only a limited number of doses administered at intervals chosen without regard for their pharmacodynamic effects of pharmacokinetic profiles. we compared the relative efficacy and potency of three beta-lactams and two aminoglycosides in lung and thigh-infection models in neutropenic mice by defining the maximum attainable antimicrobial effect at 24 h (emax) and the total dose required to reach 50% of maximum effect (p50) at several dosing interva ... | 1989 | 2644371 |
| impairment of phagocytosis by the klebsiella pneumoniae mannose-inhibitable adhesin-t7 receptor. | it has been previously shown that klebsiella pneumoniae k59 carrying the mannose-inhibitable adhesin-t7 receptor (miat) efficiently binds to polymorphonuclear leukocytes (pmns) incubated at 4 degrees c but is not efficiently bound and internalized by phagocytes incubated at 37 degrees c. pretreatment of k59 with compounds that bind the miat ligand (d-mannose, uv-inactivated t7 phages, and pepsin-digested anti-miat antibodies) enables pmns to phagocytize and kill these bacteria. in this article, ... | 1989 | 2645216 |
| a multicenter, double-blind, trimethoprim-sulfamethoxazole controlled study of enoxacin in the treatment of patients with complicated urinary tract infections. | in a double-blind, randomized, controlled trial, 249 patients with complicated urinary tract infections received either 400 mg. enoxacin or 160 mg. trimethoprim plus 800 mg. sulfamethoxazole orally every 12 hours for 14 days. the clinical outcome at the end of treatment revealed that all 89 evaluable patients (100 per cent) in the enoxacin group and 88 of 90 (98 per cent) in the trimethoprim-sulfamethoxazole group had satisfactory clinical responses (cure or improvement). bacteriological effecti ... | 1989 | 2645421 |
| subacute osteomyelitis presenting as bone tumors. | the problem of the differential diagnosis between subacute osteomyelitis and primary bone tumors is a difficult one. presenting symptoms, duration of disease, laboratory data, and location of the pathologic process are of little assistance. the authors reviewed eight typical cases. preoperative diagnosis included benign and malignant bone tumors, as well as osteomyelitis; but the final diagnosis of osteomyelitis was made only after open biopsy and culture. the presenting symptoms and signs inclu ... | 1989 | 2646622 |
| [the rapid identification of escherichia coli using bactident e. coli]. | 165 (93.7%) of 175 e. coli strains isolated from various materials of different animal species were correctly identified as e. coli with help of commercially available bactident e. coli (e. merck, d-6100 darmstadt). further 52 strains of the family of enterobacteriaceae (2x providencia sp., 2x salmonella sp., 7 citrobacter sp., 9 proteus sp., 15 klebsiella sp., 17 enterobacter sp.) were correctly not identified as e. coli by this test. bactident e. coli is a suitable test for rapid identificatio ... | 1989 | 2647078 |
| purification and properties of two succinic semialdehyde dehydrogenases from klebsiella pneumoniae. | two forms of succinic semialdehyde dehydrogenase have been isolated in klebsiella pneumoniae m5a1. the two enzymes could be separated by filtration on sephacryl s-300 and their apparent molecular weights were approx. 275,000 and 300,000. the large enzyme is specific for nadp. the smaller enzyme, which is induced by growth on 3-hydroxyphenylacetic acid, 4-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid and gamma-aminobutyrate, has been purified to 96% homogeneity by affinity chromatograp ... | 1989 | 2647149 |
| protective effects of murine monoclonal antibodies in experimental septicemia: e. coli antibodies protect against different serotypes of e. coli. | murine monoclonal antibodies that bind outer membrane antigens of the j5 mutant of escherichia coli o111:b4 were derived from spleen cells of balb/c mice immunized with killed whole cells and boosted with lipopolysaccharide (lps) and lps-associated proteins. seven hybridomas were selected for their reactivity against the j5 lps; they cross-reacted with o111, o55, o127, and o128 e. coli lps. one (b7b3) also reacted with the serratia marcescens lps and klebsiella pneumoniae lipid a. a protective e ... | 1989 | 2647860 |
| klebsiella pneumoniae endocarditis. | | 1989 | 2647988 |
| [streptococcus group b tardive meningitis revealing chronic septic granulomatosis]. | a first-born boy operated on d20 for an abscess of the anal margin (e. coli, klebsiella) developed purulent meningitis due to a group b serotype iii streptococcus on d35. the outcome was rapidly fatal. results of immunologic investigations done on the day before death were suggestive of chronic granulomatous disease (cgd) as complete absence of reduction of nitroblue tetrazolium (nbt) was evidenced. studies of polymorphonuclear cells from the mother showed normal production of oxygen, chemilumin ... | 1989 | 2648937 |
| intermittent urethral catheterization in the elderly. | thirty-five elderly patients receiving intermittent urethral catheterization in a veterans administration hospital and attached nursing home care unit were prospectively studied for development of bacteriuria and/or urinary tract infection. thirty-one of the 35 patients (88.6%) developed urinary tract colonization. the mean time from initiation of catheterization to development of colonization was 5.7 +/- 1.3 days. persistent bacteriuria with one or several different microorganisms developed in ... | 1989 | 2649539 |
| polymicrobial bacteremia: clinical and microbiologic patterns. | between 1971 and 1987, 97 patients with polymicrobial bacteremia (pmb) were seen by a consulting infectious disease service. seventy-four had severe underlying illnesses, and infection was hospital acquired in 80. pmb resulted from intraabdominal, urinary tract, or soft tissue infection in 45 patients, but a wide range of sources were implicated in the rest. eleven patients had more than one source for the bacteremia, and, despite intensive diagnostic efforts, 24 had no identifiable source for a ... | 1989 | 2649955 |
| the influence of the culture ph value on the direct glucose oxidative pathway in klebsiella pneumoniae nctc 418. | klebsiella pneumoniae nctc 418 was cultured aerobically in chemostat cultures (d = 0.3 h-1; 35 degrees c) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. the effect of the external ph value on glucose metabolism and on the enzymes of the direct glucose oxidative pathway was examined. the ph value of the medium had a profound influence on both the activity and the synthesis of the glucose dehydrogenas ... | 1989 | 2650650 |
| reduction of cyclopropene by nifv- and wild-type nitrogenases from klebsiella pneumoniae. | the nitrogenase from wild-type klebsiella pneumoniae reduces cyclopropene to cyclopropane and propene in the ratio 1:2 at ph 7.5. we show in this paper that the nitrogenase from a nifv mutant of k. pneumoniae also reduces cyclopropene to cyclopropane and propene, but the ratio of products is now 1:1.4. however, both nitrogenases exhibit the same km for cyclopropene (2.1 x 10(4) +/- 0.2 x 10(4) pa), considerably more than the km for the analogous reaction with azotobacter vinelandii nitrogenase u ... | 1989 | 2650681 |
| relationship between bacteria and ige. | in this preliminary report, we describe our inability to induce ige antibody to a well-characterized bacterial component, the membrane proteoglycan of klebsiella pneumoniae and its major protein fraction fiii-b in balb/c mice. in contrast, an immunomodulatory effect of membrane proteoglycan of k. pneumoniae on the ige and igg antibody responses to ovalbumin could be demonstrated. | 1989 | 2651316 |
| in vivo selection of a cephamycin-resistant, porin-deficient mutant of klebsiella pneumoniae producing a tem-3 beta-lactamase. | | 1989 | 2651531 |
| protracted diarrhea: the importance of the enteropathogenic e. coli (epec) strains and salmonella in its genesis. | the etiology of the protracted diarrhea is ill-defined, but in the underdeveloped countries acute gastroenteritis might be the most common triggering factor, especially due to certain enteropathogenic bacteria, such as enteropathogenic e. coli (epec) and salmonella. we investigated the role of these agents in the genesis of protracted diarrhea in 29 infants with a mean age of 4.6 months. the patients underwent the following tests: stool culture, culture of the jejunal secretion, and small bowel ... | 1989 | 2651634 |
| [clinically relevant pathogens isolated from blood cultures of inpatient treated children (1972 to 1985)]. | blood cultures are extremely useful in diagnosing various bacteremic diseases in childhood. the results obtained in this study are presented in relation to two periods of equal length (1972-1978, 1979-1985). at the present time there are about 22 relevant isolates from blood cultures per 1000 admitted children and year in the department of pediatrics, and about 3 isolates per 1000 admitted children and year in the department of pediatric surgery, respectively. from newborns we isolated most freq ... | 1989 | 2652038 |
| perinephric abscess in pediatric patients: report of six cases and review of the literature. | | 1989 | 2652088 |
| [selection of physiologic bacterial flora by chemotherapeutic agents]. | a variety of antimicrobial agents has been shown to induce alterations in the bacterial homeostasis of the human microflora. although the role of the normal flora is still poorly understood, there is evidence that alterations in the flora have a number of important clinical consequences. the normal flora acts as a natural defence against colonization or infection with pathogens. in addition, the altered flora may assume importance as a reservoir of potential pathogens. thus, antibiotic-induced c ... | 1989 | 2652892 |
| effect of etoposide and cyclophosphamide on an experimental pulmonary infection in mice. | the effects of monocytopenia and granulocytopenia on the proliferation of klebsiella pneumoniae and the efficacy of treatment with ceftriaxone were studied in an experimental lung infection in mice. treatment with etoposide led to monocytopenia, and cyclophosphamide to granulocytopenia and monocytopenia. both drugs gave a decrease in the number of alveolar macrophages during the infection and a diminished influx of granulocytes into the alveolar space. the mice treated with etoposide did not dif ... | 1989 | 2653009 |
| chromosomal beta-lactamase of klebsiella oxytoca, a new class a enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics. | the chromosomally encoded beta-lactamase gene of klebsiella oxytoca e23004, a strain resistant to cefoperazone and aztreonam, was cloned and expressed in escherichia coli hb101. the molecular mass and pi of this enzyme were 28 kilodaltons and 7.4, respectively. although the beta-lactamase of k. oxytoca hydrolyzed many cephalosporins, including broad-spectrum drugs, the nucleotide sequence and deduced amino acid sequence lacked homology with chromosomal class c beta-lactamase genes (ampc) of e. c ... | 1989 | 2653216 |
| tissue penetration and clinical efficacy of enoxacin in urinary tract infections. | the fluoroquinolones in general, and particularly enoxacin, show great promise in the treatment of urinary tract infection. orally administered enoxacin achieves high concentrations in the serum and urine as well as in prostate tissue, kidney and perirenal fat and muscle. these concentrations are generally in excess of the minimum inhibitory concentrations (mic) for 95% of the common uropathogens, including escherichia coli, pseudomonas aeruginosa, klebsiella spp., proteus spp., enterobacter spp ... | 1989 | 2653693 |
| comparative study of three different dosing regimens of cefotaxime for treatment of gram-negative bacteremia. | thirty-one patients with gram-negative bacteremia with organisms susceptible to cefotaxime (ctx) (mic of 1 microgram/ml or less) were randomized to receive 2 g of ctx every 6, 8, or 12 hr. five-hour susceptibility studies were performed on a bacterial pellet obtained from the patient's positive blood culture vial. thus, patients were enrolled within hours after gram-negative organisms were demonstrated in their blood cultures. all bacteremias were cleared although two patients had unsatisfactory ... | 1989 | 2653713 |
| synergistic interaction of cefotaxime and its metabolite desacetylcefotaxime demonstrated by drug-impregnated disks. | because no simple in vitro test for cefotaxime (ctx) and desacetylcefotaxime (dctx) synergy exists, and, because no disk susceptibility data is available for dctx, we investigated the potential of antibiotic impregnated disks as a test for positive in vitro interaction. results of this study indicate that all strains of staphylococcus aureus tested demonstrated additive interactions using disks that contained 30 micrograms of dctx. in contrast, it was necessary to use much smaller concentrations ... | 1989 | 2653715 |
| extracellular product of nocardia amarae induces bacterial cell flocculation. | the fact that nocardia amarae yk1 produced a bacterial flocculation-inducing substance (designated as fix) was discovered. fix had a function of flocculating proliferous cells. fix-induced flocculation was inhibited by making cells resting, but not completely by adding chloramphenicol. fix worked widely on gram-positive to -negative bacteria. in the presence of fix, achromobacter cycloclastus iam1013, acinetobacter calcoaceticus iam1517, bacillus subtilis iam1069, escherichia coli c600-1, e. col ... | 1989 | 2653957 |
| prophylactic co-trimoxazole versus norfloxacin in neutropenic children--perspective randomized study. | co-trimoxazole or norfloxacin were randomly administered to 44 granulocytopenic children with malignancies in order to prevent bacterial infections. although more patients in the co-trimoxazole group had febrile episodes (p less than 0.01), the mean of febrile days and the mean of days with systemic antibiotics did not differ significantly in the two groups. five patients in the co-trimoxazole group had a microbiologically documented infection (four with septicemia) due to escherichia coli (n = ... | 1989 | 2654019 |
| klebocin activity of klebsiella pneumoniae strains and its heterologous effect on staphylococcus sp. | thirty six klebsiella pneumoniae strains were assayed in a test to determine bacterial interference; two of them had bacteriocinic effects on homologous species, strains 6 and 150 inhibited sensitive k. pneumoniae and they also acted on positive and negative staphylococci coagulase. all cocci were sensitive to the bacteriocinogenic k. pneumoniae and none of the non-bacteriocinogenic strains inhibited them. klebocin with homologous activity on k. pneumoniae seemed to be undistinguishable from the ... | 1989 | 2654571 |
| intestinal decontamination for control of nosocomial multiresistant gram-negative bacilli. study of an outbreak in an intensive care unit. | to study the efficacy of intestinal decontamination by oral nonabsorbable antibiotic agents to control a nosocomial outbreak of intestinal colonization and infection with multiresistant enterobacteriaceae, and to examine its effects on endemic nosocomial infection rates. | 1989 | 2655521 |
| estimation of nitrogenase activity in the presence of ethylene biosynthesis by use of deuterated acetylene as a substrate. | nitrogenase reduces deuterated acetylene primarily to cis dideuterated ethylene. this can be distinguished from undeuterated ethylene by the use of fourier transform infrared spectroscopy. characteristic bands in the region from 800 to 3,500 cm-1 can be used to identify and quantitate levels of these products. this technique is applicable to field studies of nitrogen fixation where ethylene biosynthesis by plants or bacteria is occurring. we have verified the reaction stoichiometry by using kleb ... | 1989 | 2655535 |
| purification and characterization of a novel type of catalase from the bacterium klebsiella pneumoniae. | a novel type of catalase, designated kpa, was purified from the bacterium klebsiella pneumoniae. the enzyme is unique in that it is a dimer with subunit molecular weight of 80,000, it bears a chlorine-type heme as prosthetic group, and is active over a very wide range of h+ concentrations, with a plateau from ph 2.8 to 11.8. yet, some properties of kpa are characteristic of typical catalases: it is stable when treated with with ethanol/chloroform, cannot be reduced by dithionite and it is inhibi ... | 1989 | 2655713 |
| 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenases of escherichia coli c and klebsiella pneumoniae m5a1 show very high n-terminal sequence homology. | 5-carboxymethyl-2-hydroxymuconic semialdehyde (chms) dehydrogenase from escherichia coli c and klebsiella pneumoniae m5a1 have been purified and some of their properties studied. the apparent km values for nad and chms were 11.7 +/- 1.5 microm and 5.2 +/- 1.9 microm, respectively, for the k. pneumoniae enzyme, and 19.5 +/- 2.7 microm and 9.2 +/- 1.4 microm, respectively, for the e. coli enzyme. both enzymes were optimally active at ph 7.5 in sodium phosphate buffer. they had subunit molecular we ... | 1989 | 2656390 |
| review of the in-vitro spectrum and characteristics of cefmetazole (cs-1170). | the in-vitro antimicrobial qualities of cefmetazole are summarized from a review of over 30 publications. cefmetazole, a 7 alpha-methoxy cephalosporin, is shown to have an antimicrobial spectrum closely resembling cefoxitin's. however, cefmetazole is approximately two- to eight-fold more active than cefoxitin against commonly isolated species such as escherichia coli, klebsiella spp., proteus mirabilis, staphylococcus aureus, pyogenic streptococci, pneumococci, and haemophilus influenzae. cefmet ... | 1989 | 2656622 |
| n2o reduction and hd formation by nitrogenase from a nifv mutant of klebsiella pneumoniae. | dinitrogenase from a nifv mutant of klebsiella pneumoniae contains an altered form of iron-molybdenum cofactor (femoco) that lacks a biologically active homocitric acid molecule. change in the composition of femoco led to substantial variation in the kinetics of nitrogenase action. the kms of the mutant enzyme for n2 and n2o were 0.244 and 0.175 atm (24,714 and 17,726 kpa), respectively. the km for n2 was higher and the km for n2o was lower than that for the wild-type enzyme. the mutant enzyme w ... | 1989 | 2656643 |
| unusual nucleotide arrangement with repeated sequences in the escherichia coli k-12 chromosome. | between 59 and 60 min on the escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. about 24 kilobase pairs downstream of the 14 repeats, a similar 29-base-pair sequence with a spacing of 32 base pairs appears seven times. nucleotide sequences hybridizing with the 29-base-pair fragment were also detected in shigella dysenteria ... | 1989 | 2656660 |
| computed tomography of renal abscesses complicating medullary sponge kidney. | although urinary tract infection occurs in approximately one-third of symptomatic patients with medullary sponge kidney (msk), renal abscess is a rare complication of the disorder. we describe two patients with msk complicated by renal abscesses that were diagnosed by ct. the diagnosis of renal abscess should be suspected in patients with msk and acute pyelonephritis who do not respond to appropriate antibiotic therapy, and renal ct should be done. computed tomography may show large abscesses th ... | 1989 | 2656783 |
| klebsiella pneumoniae bacteremia in the community hospital. | the purpose of this study was to elicit the circumstances of occurrence and organism sensitivities of klebsiella pneumoniae bacteremia in the community hospital, since data on this illness from the community hospital are rare. all records of documented klebsiella pneumoniae bacteremia (46 cases) at alachua general hospital, gainesville, florida, over the period july 14, 1982, through july 27, 1985, were reviewed in detail. fifty-nine percent (27 organisms) were nosocomial, whereas 41% (19 organi ... | 1989 | 2656904 |
| successful treatment of metastatic endophthalmitis. case reports. | endophthalmitis is a well-recognized complication of intraocular surgery, penetrating ocular trauma and systemic infection. metastatic bacterial endophthalmitis is rare. however, once it happens, the visual outcome is very poor. in order to prevent visual damage, early diagnosis and treatment is essential. due to the blood-ocular barrier, intravitreal drug concentrations are low after systemic administration. strong antibiotics with good penetration into the vitrous humor are needed to obtain ad ... | 1989 | 2657535 |
| immunomodulating drugs in pneumology. | | 1989 | 2657918 |
| suppurative thrombophlebitis of the superior vena cava. | a 22-year-old woman, a neurosurgical comatose patient developed suppurative thrombophlebitis of the superior vena cava due to klebsiella pneumoniae, as a complication of catheterisation for parenteral nutrition. the diagnosis was established by gallium scan, computed tomography and digital vascular imaging. conservative treatment with antibiotics and heparin resulted in the emergence of a resistant mutant of the causative agent. the infection could only be eradicated after surgical thrombectomy. | 1989 | 2658015 |
| [isolation and characteristics of klebsiella pneumoniae lysozyme factor]. | for the first time the preparation of k. pneumoniae antilysozyme factor has been isolated and purified. this factor, having a molecular weight of about 1,000 daltons, is oligopeptide with an oligosaccharide part. it also contains such amino acids as proline, lysine, arginine and tyrosine. the antilysozyme factor is resistant to 30-minute boiling and has no optimal ph value for its action. | 1989 | 2658433 |
| mucociliary disease of the middle ear during experimental otitis media with effusion induced by bacterial endotoxin. | lipopolysaccharide (10 micrograms/ml) derived from klebsiella pneumoniae was injected into the middle ear of guinea pigs. the animals were killed painlessly on days 1, 3, and 7 after inoculation, and the mucosal samples from two sites within the tympanic cavity, close to the tympanic orifice and distal to the orifice, were examined for ciliary activity and epithelial morphology. at day 1 and day 3 serous effusion was observed and deterioration of ciliary activity and morphologic changes were obs ... | 1989 | 2658718 |
| effects of microamperage, medium, and bacterial concentration on iontophoretic killing of bacteria in fluid. | prevention of nosocomial urinary tract infections by iontophoresis is addressed. an iontophoretic generator was used to provide microamperage (10 to 400 microa) to vials containing either synthetic urine or supplemented synthetic urine. bacteria were added to vials, and parameters of growth, bacterial killing, and multiple electrode materials were examined. escherichia coli and proteus species were both inhibited and killed at various microamperages and with several electrode types, the most eff ... | 1989 | 2658791 |
| effect of carbonyl cyanide m-chlorophenylhydrazone on escherichia coli halotolerance. | the growth-inhibitory effect of carbonyl cyanide m-chlorophenylhydrazone (cccp) was less on members of the family enterobacteriaceae (halotolerant organisms) than it was on species of vibrio (moderately halophilic organisms). when sodium chloride concentration increased from 0.5 to 0.85 m, this effect was more pronounced for escherichia coli; it remained relatively stable for vibrio spp. the effect of carbonyl cyanide m-chlorophenylhydrazone was antagonized by the addition of glycine betaine or ... | 1989 | 2658803 |
| prospective randomized comparison of mezlocillin therapy alone with combined ampicillin and gentamicin therapy for patients with cholangitis. | forty-six patients with cholangitis were randomized to receive therapy with mezlocillin sodium (24 patients) or a combination of ampicillin sodium--gentamicin sulfate (22 patients). the biliary concentration of mezlocillin was 112 times higher than that of ampicillin and 778 times higher than that of gentamicin. the ratio of the concentration in serum or bile over the minimum inhibitory concentration against aerobic gram-negative bacilli (therapeutic index) was higher for mezlocillin than for ei ... | 1989 | 2658897 |
| klebsiella pneumoniae liver abscess associated with septic endophthalmitis. | | 1989 | 2658901 |
| intrarenal abscess caused by klebsiella pneumoniae in a neonate: modern management and diagnosis. | we recently cared for a 12-day-old infant who was hospitalized for renal abscess caused by klebsiella pneumoniae, an organism not identified as etiologic in the three newborns described to date with this disorder. the advent of cross-sectional diagnostic imaging techniques, specifically ultrasonography and computed tomography (ct), has changed the management of renal abscess in children and neonates. ultrasonography is the diagnostic procedure of choice, but ct is another reliable imaging modali ... | 1989 | 2659021 |
| escherichia coli serotype-39 capsular polysaccharide: primary structure and depolymerisation by a bacteriophage-associated glycanase. | the acidic capsular polysaccharide isolated from escherichia coli o9:k39:h9 was investigated, using n.m.r. spectroscopy, methylation analysis, uronic acid degradation of the native and methylated polysaccharides, and bacteriophage-associated enzyme degradation. the structure of the repeating unit, which is shown below, is identical to that reported for klebsiella serotype-61 capsular polysaccharide. (formula; see text) | 1989 | 2659169 |
| aztreonam in the treatment of urinary tract infections: a multicenter trial. | a multicenter trial involving several urologic units in italy provided pooled data on 1,427 patients with urinary tract infections to evaluate the efficacy and safety of aztreonam, a new monobactam antibiotic. microbiologic and clinical data were collected methodically in all cases. a majority of the patients (79.9%) were hospitalized during the study period, an associated pathology was noted in 29.1%, and 16.7% were receiving additional therapy. aztreonam was administered by different routes an ... | 1989 | 2659294 |
| a comparative analysis of aztreonam + clindamycin versus tobramycin + clindamycin or amikacin + mezlocillin in the treatment of gram-negative lower respiratory tract infections. | one hundred ten patients were randomized to receive one of the following antibiotic combinations: aztreonam + clindamycin, tobramycin + clindamycin, or amikacin + mezlocillin for the treatment of lower respiratory tract infections (lrti) caused by gram-negative bacilli. of the 68 patients who received aztreonam + clindamycin, 60 were clinically evaluable and 50 were bacteriologically evaluable. of the 60 clinically evaluable patients, 54 were cured and 5 were treatment failures or died during th ... | 1989 | 2659296 |
| resistotyping as a new epidemiological marker for klebsiella pneumoniae. | resistogram typing was established with the help of 30 randomly chosen clinical isolates of k. pneumoniae using sodium arsenate, malachite green, boric acid, potassium tellurite, mercuric chloride, antimony potassium tartarate and sodium arsenite. the resistance to these chemicals was designated as a, b, c, d, e, f and g respectively. the technique was then applied to 152 clinical isolates of k. pneumoniae. a total of 35 patterns were obtained. common patterns were abefg and abfg. there was no c ... | 1989 | 2661423 |
| klebsiella pneumoniae puls gene encodes an outer membrane lipoprotein required for pullulanase secretion. | the product of the klebsiella pneumoniae gene puls, which is located downstream from the pullulanase structural gene (pula), is essential for the cell surface localization and extracellular release of pullulanase in escherichia coli k-12. puls is transcribed in the opposite direction to pula, from which it is separated by a region of 624 nucleotides. although this latter region contains a new component of the maltose regulon, pulb, which is transcribed from the pula promoter, it is not required ... | 1989 | 2661532 |
| comparison of identification of enterobacteriaceae by api 20e and sensititre autoidentification system. | of 251 isolates of the enterobacteriaceae identified to species level by api 20e, 208 (83%) were similarily identified by the sensititre autoidentification system. both systems shared a common problem in that discrimination between species of the genera klebsiella, enterobacter, and serratia was poor. the eight digit biocode generated by the sensititre system for individual isolates is not reproducible and therefore not of epidemiological value. | 1989 | 2661593 |
| emphysematous pyelonephritis. | a case of emphysematous pyelonephritis with gas formation extending into the renal vein is presented. diagnostic procedures and aggressive surgical management are discussed. | 1989 | 2661615 |
| a case of infectious pseudoaneurysm of the abdominal aorta associated with infectious spondylitis due to klebsiella pneumoniae. | a 67-year-old woman with pneumonia and diabetes mellitus was admitted with the complaints of abdominal and back pain. sputum culture was positive for klebsiella pneumoniae. computed tomographic scanning (ct) of the abdomen and spinal radiograph of the lumber column revealed a paraventebral space-occupying lesion, abdominal aortic aneurysm and destructive change of l3 and l4. pseudoaneurysm of the abdominal aorta associated with infectious spondylitis with paravertebral abscess was suspected and ... | 1989 | 2661892 |
| microcalorimetric studies of the effects of platinum group metal complexes on bacterial growth. | a range of platinum group metal complexes (pgmc) were screened for antibacterial activity against klebsiella aerogenes and staphylococcus aureus. the effects of the complexes were monitored by changes in the thermal and growth properties of the organisms. active complexes caused an immediate cessation of power and biomass production which did not recover whilst the complex was present in the medium. removal of the complex by washing the cells allowed the growth of the small viable proportion of ... | 1989 | 2661974 |
| immunological activities of ru-41740, a glycoprotein extract from klebsiella pneumoniae. iii. role of lps-like and lps-non-related molecules. | ru-41740, a glycoprotein complex extracted from klebsiella pneumoniae, is an immunomodulating agent which acts on b cells and macrophages. it has been shown that ru-41740 is composed mainly of two macromolecular fractions, f1, having an lps-related structure, and p1, with a proteoglycan structure. in the present paper, the effects of these molecules on b cells and on il-1 and tumour necrosis factor (tnf), production by macrophages were compared. data reveal that both fractions were mitogenic for ... | 1989 | 2662280 |
| [combined chemotherapy of experimental infection in neutropenia]. | a significant decrease in resistance to infections caused by gramnegative pathogens was observed in mice with neutropenia induced by cytostatics. efficacy of schemes for combined chemotherapy with beta-lactams, aminoglycosides and a novel peptide antibiotic was studied on model infections in mice with neutropenia. in the neutropenic mice with sepsis caused by pseudomonas the peptide antibiotic administered parenterally in a single dose of 50 micrograms/kg provided high therapeutic activity. in c ... | 1989 | 2662929 |
| homocitrate is a component of the iron-molybdenum cofactor of nitrogenase. | when apodinitrogenase (lacking femo-co) was activated with femo-co synthesized in vitro in the presence of 3h-labeled homocitrate, label was incorporated into dinitrogenase. the physical association of the label with femo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. the presence of homocitrate in femo-co was established by nmr analysis of the organic acid extracted from dinitrogenase. quantitation of homocitrate in dinitrogenase showed it to be present ... | 1989 | 2663059 |
| comparative activity of cefoxitin, ampicillin/sulbactam, and imipenem against clinical isolates of escherichia coli and klebsiella pneumoniae. | the in vitro activities of cefoxitin, ampicillin/sulbactam, and imipenem were determined by the standard twofold agar dilution method against 62 strains of escherichia coli and 40 strains of klebsiella pneumoniae isolated from patients in intensive care units. judging from the concentrations required to inhibit at least 90% of the test isolates, imipenem (mic90 less than or equal to 0.125 micrograms/ml) was markedly more active than cefoxitin (mic90 = 4 micrograms/ml) and ampicillin/sulbactam (m ... | 1989 | 2663161 |