| fluorescence lifetime analysis of nitrite reductase from alcaligenes xylosoxidans at the single-molecule level reveals the enzyme mechanism. | | 2011 | 21922585 |
| orientational control over nitrite reductase on modified gold electrode and its effects on the interfacial electron transfer. | recently, studies have been reported in which fluorescently labeled redox proteins have been studied with a combination of spectroscopy and electrochemistry. in order to understand the effect of the dye on the protein-electrode interaction, voltammetry and surface analysis have been performed on protein films of dye-labeled and unlabeled forms of a cysteine-surface variant (l93c) and the wild type (wt) of the copper containing nitrite reductase (nir) from alcaligenes faecalis s6. the protein h ... | 2011 | 21939276 |
| Synthesis and biochemical analysis of 2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoro-N-hydroxy-octanediamides as inhibitors of human histone deacetylases. | Inhibition of human histone deacetylases (HDACs) has emerged as a novel concept in the chemotherapeutic treatment of cancer. Two chemical entities, SAHA (ZOLINZA, Merck) and romidepsin (Istodax, Celgene) have been recently approved by the FDA as first-in-class drugs against cutaneous T-cell lymphoma. Clinical use of these drugs revealed several side effects including gastro-intestinal symptoms, fatigue, thrombocytopenia, thrombosis. Romidepsin is associated with an yet unresolved cardiotoxicity ... | 2011 | 22182579 |
| Effect of physicochemical parameters on enzymatic biodecaffeination during tea fermentation. | We report for the first time the development of a biodecaffeination process for tea synchronised with tea fermentation process using enzymes isolated from Pseudomonas alcaligenes. Cell-free extract was used for biodecaffeination of tea during fermentation of tea and 80% of the caffeine in the tea dhool was degraded within 90 min of incubation. Several factors that tend to effect the biodecaffeination during this stage, like moisture, aeration, intermittent enzyme addition and mixing, were optimi ... | 2011 | 22116671 |
| alcaligenes xylosoxidans contact lens-related keratitis--a case report and literature review. | alcaligenes xylosoxidans can result in opportunistic infections in patients who are immunocompromised. alcaligenes xylosoxidans keratitis is a rare infection, almost always occurring in a compromised cornea. few reported cases have occurred as contact lens-induced keratitis. the purposes of this report are to report a case of a. xylosoxidans keratitis in an immunocompetent contact lens wearer (2-week disposable) and to review the related literature. | 2011 | 21993586 |
| Relationship of cell-wall bound fatty acids and the demulsification efficiency of demulsifying bacteria Alcaligenes sp. S-XJ-1 cultured with vegetable oils. | Considering that the surface properties of demulsifying cells correlate with their demulsification efficiency, the demulsifying bacteria Alcaligenes sp. S-XJ-1 with various surface properties were obtained using different vegetable oils as carbon sources. The results show that better performance was achieved with demulsifying bacteria S-XJ-1 possessing a relatively high cell surface hydrophobicity (CSH) and total unsaturated degree for the cell-wall bound fatty acids. There also appeared to be a ... | 2012 | 22093975 |
| biodegradation kinetics of highly chlorinated biphenyls by alcaligenes sp. jb1 in an aerobic continuous culture system. | | 1995 | 22148213 |
| identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis candidates. | to describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ... | 2012 | 22074685 |
| Cutinolytic esterase activity of bacteria isolated from mixed-plant compost and characterization of a cutinase gene from Pseudomonas pseudoalcaligenes. | The objective of the current study was to examine cutinolytic esterase (i.e., cutinase) activity by pseudomonads and bacteria isolated from mixed-plant compost. Approximately 400 isolates representing 52 taxa recovered from mixed-plant compost using cuticle baits, along with 117 pseudomonad isolates obtained from a culture collection (i.e., non-compost habitats), were evaluated. The ability of isolates to degrade the synthetic cutin polycaprolactone (PCL) was initially measured. Isolates from 23 ... | 2011 | 22029433 |
| development of an immunochromatographic assay for diagnosing the production of imp-type metallo-β-lactamases that mediate carbapenem resistance in pseudomonas. | rapid and reliable detection of carbapenem-resistant bacteria is an important infection-control measure and a crucial aspect of antimicrobial chemotherapy. imp-type metallo-β-lactamase (mbl) is an emzyme that mediate carbapenem resistance in bacteria. here, an immunochromatographic assay was newly developed using novel monoclonal antibodies (mabs) recognizing imp-type mbl. epitope mapping of mabs and mutational analysis of the epitope region in imp antigen suggested that the mabs could react to ... | 2011 | 21986031 |
| gene cloning, expression, and characterization of a nitrilase from alcaligenes faecalis zjutb10. | nitrilases are important industrial enzymes that convert nitriles directly into the corresponding carboxylic acids. in the current work, the fragment with a length of 1068 bp that encodes the a. faecalis zjutb10 nitrilase was obtained. moreover, a catalytic triad was proposed and verified by site-directed mutagenesis, and the detailed mechanism of this nitrilase was clarified. the substrate specificity study demonstrated that the a. faecalis zjutb10 nitrilase belongs to the family of arylacetoni ... | 2011 | 21913706 |
| application of waste frying oils in the biosynthesis of biodemulsifier by a demulsifying strain alcaligenes sp. s-xj-1. | exploration of biodemulsifiers has become a new research aspect. using waste frying oils (wfos) as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the application of biodemulsifiers in the oilfield. in this study, a demulsifying strain, alcaligenes sp. s-xj-1, was investigated to synthesize a biodemulsifier using waste frying oils as carbon source. it was found that the increase of initial ph of culture medium could increase the bio ... | 2011 | 22066226 |
| microbiological contamination of spirometers - an exploratory study in general practice. | background spirometry is an important tool when diagnosing chronic respiratory conditions in general practice. however, the equipment may harbour pathogenic micro-organisms and cross-transmission of aerolised pathogens could occur if hygiene measures are insufficient. methods we assessed microbiological contamination in 16 spirometers from a convenience sample of south australian general practices. results we found potentially relevant microbiological contamination in three spirometers: two ps ... | 2012 | 22276288 |
| onpa, an unusual flavin-dependent monooxygenase containing a cytochrome b5 domain. | ortho-nitrophenol 2-monooxygenase (ec 1.14.13.31) from alcaligenes sp. strain nyz215 catalyzes monooxygenation of ortho-nitrophenol to form catechol via ortho-benzoquinone. sequence analysis of this onpa-encoded enzyme revealed that it contained a flavin-binding monooxygenase domain and a heme-binding cytochrome b5 domain. onpa was purified to homogeneity as a his-tagged protein, and was considered to be a monomer as determined by gel filtration. fad and heme were identified by hplc (high-perfor ... | 2012 | 22267507 |