| q fever in young children, ghana. | | 2008 | 18258140 |
| [q fever]. | q fever is a zoonosis caused by coxiella burnetii. the infection is transmitted to humans mainly through aerosols generated from products of delivery of cows and other animals. generally the disease presents acutely with fever, pneumonitis and hepatitis. but chronic endocarditis is also a possible occurrence. diagnosis is usually made by serology. the acute form of the infection responds well to tetracyclines and other antibiotics. but endocarditis is difficult to treat, and its prognosis is gri ... | 2007 | 18275268 |
| infection of human monocyte-derived macrophages with coxiella burnetii. | coxiella burnetii, the agent of q fever, is an obligate intracellular bacterium that has a tropism for cells of the mononuclear phagocyte system. following internalization, c. burnetii remains in a phagosome that ultimately matures into a vacuole with lysosomal characteristics that supports pathogen replication. most in vitro investigations of coxiella - macrophage interactions have employed continuous cell lines. although these studies have been informative, genetic alterations of immortalized ... | 2008 | 18287757 |
| diagnosis and treatment of q fever: attempts to clarify current problems in japan. | "q fever" is a generic term for infection caused, mostly in the form of pneumonia or bronchitis, by coxiella burnetii (q-fever coxiella), a pathogen closely related to rickettsia and legionella. q fever is an influenza-like, transient febrile infectious disease that is common to humans and animals; it develops after the transmission of the infectious agent from livestock or pet animals, but person-to-person transmission is rare. in europe and the united states, it is ranked fourth or fifth as an ... | 2008 | 18297442 |
| [a 42-year-old farmer with nonspecific leucocytosis and elevated transaminases. acute septic reaction in coxiella burnetii infection]. | we report about a 42-year-old farmer with leucocytosis, elevation of transaminases and liver cirrhosis as an underlying condition. the diagnosis of q fever hepatitis was made through liver biopsy and serology. under therapy with doxycycline, transaminases initially increased again; after switching to ciprofloxacin, the patient could be discharged 3 weeks after admission. q fever is caused by coxiella burnetii. the most frequent acute manifestation is a self-limiting flu-like illness. chronic q f ... | 2008 | 18309471 |
| sustained axenic metabolic activity by the obligate intracellular bacterium coxiella burnetii. | growth of coxiella burnetii, the agent of q fever, is strictly limited to colonization of a viable eukaryotic host cell. following infection, the pathogen replicates exclusively in an acidified (ph 4.5 to 5) phagolysosome-like parasitophorous vacuole. axenic (host cell free) buffers have been described that activate c. burnetii metabolism in vitro, but metabolism is short-lived, with bacterial protein synthesis halting after a few hours. here, we describe a complex axenic medium that supports su ... | 2008 | 18310349 |
| limited role for iron regulation in coxiella burnetii pathogenesis. | in gram-negative bacteria, iron acquisition proteins are commonly regulated by fur (ferric uptake regulator), which binds iron-regulated promoters (the fur box). we hypothesized that coxiella burnetii requires iron and employs an iron-regulatory system and used various approaches to define a fur regulon. cloned c. burnetii fur complemented an escherichia coli fur deletion mutant. a ferrous iron transporter gene (cbu1766), a putative iron binding protein-encoding gene (cbu0970), and a cation effl ... | 2008 | 18316381 |
| bartonella native valve endocarditis: the first brazilian case alive and well. | bartonella is an important cause of blood culture-negative endocarditis in recent studies. seroprevalence studies in the states of minas gerais and rio de janeiro have shown bartonella igg positivity around 14% in healthy adults and 40% in hiv seropositive adults, respectively. a case report of a 46-year-old white male with moderate aortic regurgitation (ar) due to rheumatic heart disease (rhd), admitted due to worsening heart failure, is presented. clinical features were apyrexia, anemia, polyc ... | 2007 | 18327472 |
| q fever in returned febrile travelers. | despite the worldwide distribution of coxiella burnetii, q fever is not a frequent infection in travelers. this disease should be considered in recent travelers from the tropics with unknown fever, headache, raised liver enzymes, and low platelets. five cases in western travelers to the tropics returning with fever are presented. | 2008 | 18346248 |
| coxiella burnetii, the agent of q fever, stimulates an atypical m2 activation program in human macrophages. | coxiella burnetii is an obligate intracellular bacterium, responsible for q fever, which survives in macrophages by interfering with their microbicidal competence. as functional polarization of macrophages is critical for their microbicidal activity, we studied the activation program of monocyte-derived macrophages (mdm) stimulated with c. burnetii. this program was markedly distinct from that induced by lipopolysaccharides (lps), a canonical inducer of m1 polarization. indeed, c. burnetii up-re ... | 2008 | 18350541 |
| q fever bioprosthetic aortic valve endocarditis (pve) successfully treated with doxycycline monotherapy. | q fever is a zoonotic infection caused by coxiella burnetii. the most common clinical manifestation of acute q fever infection is as an atypical community-acquired pneumonia. the pulmonary findings are accompanied by extrapulmonary findings, most typically an increase in serum transaminases and splenomegaly. because c. burnetii is difficult to culture, the diagnosis of q fever is usually made serologically. the diagnosis of acute q fever atypical community-acquired pneumonia is made by demonstra ... | 2008 | 18371509 |
| q fever presenting as recurrent, culture-negative endocarditis with aortic prosthetic valve failure: a case report and review of the literature. | the present report describes a case of recurrent, culture-negative endocarditis presenting with aortic prosthetic valve dysfunction in a 62-year-old man who required four valve replacement surgeries. on each occasion, he presented with valve failure. fever was only documented during his first presentation. furthermore, no vegetations were detectable on his aortic valve at transesophageal echocardiography. on the occasion of his most recent presentation, a detailed history of animal exposure - in ... | 2006 | 18382649 |
| q fever-related otological manifestations following chronic exposure to products of conception? | | 2008 | 18384471 |
| human seroprevalence to coxiella burnetii (q fever) in northern ireland. | despite the widespread prevalence of infection with coxiella burnetii, there have been few large population-based studies examining the epidemiology of this infection. the aim of this study was to examine the distribution and determinants of c. burnetii past infection in northern ireland (ni). coxiella burnetii phase ii specific igg antibodies were measured by enzyme-linked immunosorbent assay in stored serum from 2,394 randomly selected subjects, aged 12-64, who had participated in population-b ... | 2008 | 18387140 |
| [epidemiological aspects of human q fever in indre-et-loire between 2003 and 2005 and comparison with caprine q fever]. | chronic infection is the major risk of q fever. c. burnetii infections result from the inhalation of contaminated aerosols. indre-et-loire is a rural french area with numerous goat farms. we evaluated human q fever epidemiology and compared it with q fever in goats. | 2008 | 18395376 |
| [q fever--a new zoonotic disease in denmark? the danish society for infectious diseases]. | | 2008 | 18397651 |
| 16s rrna-, groel- and mucz-based assessment of the taxonomic position of 'rickettsiella melolonthae' and its implications for the organization of the genus rickettsiella. | 'rickettsiella melolonthae' is an intracellularly multiplying bacterial pathogen of european cockchafers, melolontha melolontha (linnaeus, 1758) and melolontha hippocastani (fabricius, 1801) (coleoptera: scarabaeidae). we report the first determination of nucleotide sequences from this organism, i.e. the 16s rrna encoding rrs gene, the chaperonin encoding groel gene and the mucz gene encoding the orthologue of a capsule synthesis-inducing factor of coxiella burnetii. within the genus rickettsiel ... | 2008 | 18398164 |
| [q fever in hospital-acquired pneumonia]. | we studied the effects of q fever in hospital-acquired pneumonia. the subjects consisted of 121 cases with hospital-acquired pneumonia treated during the period from december 2004 till june 2007. q fever was diagnosed using a panbio coxiella burnetii elisa test kit. there were no patients with acute infection by coxiella burnetii. it is concluded that c. burnetii cannot induce onset of hospital-acquired pneumonia. | 2008 | 18409563 |
| fractionation of the coxiella burnetii parasitophorous vacuole. | coxiella burnetii is a bacterial obligate intracellular pathogen that replicates within a spacious parasitophorous vacuole (pv) with lysosomal characteristics. the pathogen actively participates in the biogenesis of its pv by synthesizing proteins that mediate vesicular interactions. both c. burnetii and host factors that regulate pv formation are likely localized to the pv membrane, and their identification would be aided by an efficient method for isolating the c. burnetii vacuole. to this end ... | 2008 | 18425464 |
| human perception and awareness of ticks in a south australian rural community and implications for management of amblyomma triguttatum triguttatum. | the ornate kangaroo tick, amblyomma triguttatum triguttatum, is invasive on yorke peninsula, south australia. to assess the potential for developing comprehensive management strategies for the species, we mailed 1,000 surveys to yorke peninsula residents to investigate community perceptions of ticks, gather information on tick-host ecology, and assess whether or not occupation and length of residence affected whether residents had seen ticks. the response rate was 32%; most respondents (63%) had ... | 2008 | 18427936 |
| comparison of pcr, immunofluorescence assay, and pathogen isolation for diagnosis of q fever in humans with spontaneous abortions. | coxiella burnetii, an obligate intracellular parasite with a worldwide distribution, is the causative agent of q fever in humans. we tested a total of 368 samples (placental bits, genital swabs, fecal swabs, and urine and serum samples) collected from women (n = 74) with spontaneous abortions for c. burnetii by a pcr assay targeting is1111, the repetitive transposon-like region of c. burnetii (trans-pcr); real-time pcr; an indirect immunofluorescence assay (ifa); and the isolation of the pathoge ... | 2008 | 18448698 |
| q fever: epidemiology, diagnosis, and treatment. | q fever, a zoonosis caused by coxiella burnetii, is seen throughout the world. recent reports suggest that its incidence in the united states is increasing, with more than 30 cases reported in the us military. the disease has many acute and chronic manifestations. endocarditis is the most common form of chronic disease, and recent studies have led to substantial changes in the approach to its diagnosis and treatment. military and civilian health care professionals need to consider q fever when e ... | 2008 | 18452690 |
| [drug activity against coxiella burnetii, a pathogen of q fever]. | | 2007 | 18461810 |
| the uptake of apoptotic cells drives coxiella burnetii replication and macrophage polarization: a model for q fever endocarditis. | patients with valvulopathy have the highest risk to develop infective endocarditis (ie), although the relationship between valvulopathy and ie is not clearly understood. q fever endocarditis, an ie due to coxiella burnetii, is accompanied by immune impairment. patients with valvulopathy exhibited increased levels of circulating apoptotic leukocytes, as determined by the measurement of active caspases and nucleosome determination. the binding of apoptotic cells to monocytes and macrophages, the h ... | 2008 | 18483547 |
| high throughput detection of coxiella burnetii by real-time pcr with internal control system and automated dna preparation. | coxiella burnetii is the causative agent of q-fever, a widespread zoonosis. due to its high environmental stability and infectivity it is regarded as a category b biological weapon agent. in domestic animals infection remains either asymptomatic or presents as infertility or abortion. clinical presentation in humans can range from mild flu-like illness to acute pneumonia and hepatitis. endocarditis represents the most common form of chronic q-fever. in humans serology is the gold standard for di ... | 2008 | 18489780 |
| "doughnut" granulomas from erythema nodosum in acute q fever. | | 2008 | 18494244 |
| rickettsial seroepidemiology among farm workers, tianjin, people's republic of china. | high seroprevalence rates for anaplasma phagocytophilum (8.8%), coxiella burnetii (6.4%), bartonella henselae (9.6%), and rickettsia typhi (4.1%) in 365 farm workers near tianjin, people's republic of china, suggest that human infections with these zoonotic bacteria are frequent and largely unrecognized. demographic features of seropositive persons suggest distinct epidemiology, ecology, and risks. | 2008 | 18507907 |
| coxiella burnetii in wild-caught filth flies. | | 2008 | 18507933 |
| high prevalence of fastidious bacteria in 1520 cases of uveitis of unknown etiology. | the etiologic evaluation of uveitis is frequently unsuccessful when noninvasive methods are used. we conducted a prospective study to evaluate systematic screening for pathogens of uveitis. all patients with uveitis referred to the participating tertiary ophthalmology departments from january 2001 to september 2007 underwent intraocular and serum specimen collection. the standardized protocol for laboratory investigations included universal polymerase chain reaction (pcr)-based detection of any ... | 2008 | 18520326 |
| incidental live-saving polymerase chain reaction in a case of prosthetic valve dual-pathogen endocarditis. | | 2008 | 18522509 |
| clinical spectrum of fever of intermediate duration in the south of spain. | fever of intermediate duration (fid) is a new nosologic entity defined as fever higher than 38 degrees c that has a duration of between 1 and 4 weeks and that after an initial approach has not been diagnosed. it has clinical similarities with fever of unknown origin, but because of characteristic etiologies it requires the term fid. we describe the clinical characteristics and etiology of fid in the south of spain and create a treatment algorithm. retrospective study of the medical charts of pat ... | 2008 | 18536946 |
| type iv secretion systems: tools of bacterial horizontal gene transfer and virulence. | type iv secretion systems (t4sss) are multisubunit cell-envelope-spanning structures, ancestrally related to bacterial conjugation machines, which transfer proteins and nucleoprotein complexes across membranes. t4sss mediate horizontal gene transfer, thus contributing to genome plasticity and the evolution of pathogens through dissemination of antibiotic resistance and virulence genes. moreover, t4sss are also used for the delivery of bacterial effector proteins across the bacterial membrane and ... | 2008 | 18549454 |
| [q fever in tunisia]. | q fever is a common zoonosis with almost a worldwide distribution caused by coxiella burnetii. farm animals and pets are the main reservoirs of infection and transmission to humans is usually via inhalation of contaminated aerosols. infection in humans is often asymptomatic, but it can manifest as an acute disease (usually a self-limited flu-like illness, pneumonia or hepatitis) or as a chronic form (mainly endocarditis, but also hepatitis and chronic-fatigue syndrome). in tunisia, although prev ... | 2009 | 18554822 |
| ankyrin repeat proteins comprise a diverse family of bacterial type iv effectors. | specialized secretion systems are used by many bacteria to deliver effector proteins into host cells that can either mimic or disrupt the function of eukaryotic factors. we found that the intracellular pathogens legionella pneumophila and coxiella burnetii use a type iv secretion system to deliver into eukaryotic cells a large number of different bacterial proteins containing ankyrin repeat homology domains called anks. the l. pneumophila ankx protein prevented microtubule-dependent vesicular tr ... | 2008 | 18566289 |
| seroprevalence of antibodies to coxiella burnetii among employees of the veterinary university in kosice, eastern slovakia. | coxiella burnetii is an obligate intracellular pathogen known to be the causative agent of q fever, a zoonosis with worldwide occurrence. the organism has been found in many wild and domestic animals. infected animals shed highly stable bacteria in urine, faeces, milk, and through placental and birth fluids. humans acquire the infection mainly by inhaling infected aerosols, or by ingesting contaminated raw milk or fresh dairy products; tick transmission has been proven but is probably rare. the ... | 2008 | 18581989 |
| prevention of coxiella burnetii shedding in infected dairy herds using a phase i c. burnetii inactivated vaccine. | the main objective of this study was to assess the efficacy of a monovalent inactivated vaccine containing phase i coxiella burnetii to prevent coxiella shedding in susceptible dairy cows within infected herds in comparison to a placebo. a total of 336 dairy cows and heifers, from six spontaneously infected herds, were followed over a 1-year period. before treatment (i.e. vaccination or placebo), the c. burnetii infection status of the cows was determined on the basis on pcr results on milk, vag ... | 2008 | 18586060 |
| lovastatin, but not pravastatin, limits in vitro infection due to coxiella burnetii. | | 2008 | 18593722 |
| bartonella quintana and coxiella burnetii as causes of endocarditis, india. | | 2008 | 18598654 |
| toxic introns and parasitic intein in coxiella burnetii: legacies of a promiscuous past. | the genome of the obligate intracellular pathogen coxiella burnetii contains a large number of selfish genetic elements, including two group i introns (cbu.l1917 and cbu.l1951) and an intervening sequence that interrupts the 23s rrna gene, an intein (cbu.dnab) within dnab and 29 insertion sequences. here, we describe the ability of the intron-encoded rnas (ribozymes) to retard bacterial growth rate (toxicity) and examine the functionality and phylogenetic history of cbu.dnab. when expressed in e ... | 2008 | 18606739 |
| [ticks and human tick-borne diseases in africa]. | ticks are obligate hematophagous arthropod parasites that feed on the blood of every class of vertebrates in almost every region of the world. tick bites can transmit bacterial, viral and parasitic diseases to humans. in this review we describe ticks and human tick-borne diseases in africa. the first part of this article presents elements linking the morphology and biology of these acarians as well as various aspects of their taxonomy and phylogeny. the next part of the article describes the mai ... | 2008 | 18630043 |
| constitutive sos expression and damage-inducible addab-mediated recombinational repair systems for coxiella burnetii as potential adaptations for survival within macrophages. | summary: coxiella burnetii, a gram-negative obligate intracellular pathogen, replicates within an parasitophorous vacuole with lysosomal characteristics. to understand how c. burnetii maintains genomic integrity in this environment, a database search for genes involved in dna repair was performed. major components of repair, sos response and recombination were identified, including reca and ruvabc, but lexa and recbcd were absent. instead, c. burnetii possesses addab orthologous genes, functiona ... | 2008 | 18647165 |
| low-dose priming before vaccination with the phase i chloroform-methanol residue vaccine against q fever enhances humoral and cellular immune responses to coxiella burnetii. | although the phase i coxiella burnetii cellular vaccine is completely efficacious in humans, adverse local and systemic reactions may develop if immune individuals are inadvertently vaccinated. the phase i chloroform-methanol residue (cmri) vaccine was developed as a potentially safer alternative. human volunteers with no evidence of previous exposure to c. burnetii received a subcutaneous vaccination with the cmri vaccine in phase i studies under protocol ind 3516 to evaluate the safety and imm ... | 2008 | 18701647 |
| detection and identification of coxiella burnetii based on the mass spectrometric analyses of the extracted proteins. | rapid and reliable detection, identification, and typing of bacterial species are necessary in response to natural or terrorist-caused outbreaks of infectious diseases and play crucial roles in diagnosis and efficient treatment. we report here two proteomic approaches with a high potential in the detection and identification of coxiella burnetii, the causative agent of q fever. the first of them starts with the acetonitrile (acn) and trichloroacetic acid extractions of inactivated c. burnetii ce ... | 2008 | 18707130 |
| recurrent pericarditis: infectious or autoimmune? | the etiology and pathogenesis of idiopathic recurrent acute pericarditis (irap) remain controversial standing like a bridge that crosses infectious, autoimmune and autoinflammatory pathways. anything may cause acute pericarditis; echo-virus, and coxsackie are the most frequently involved viruses, mycobacterium tuberculosis and coxiella burnetii the most common bacteria, but in 85% of cases it remains "idiopathic". recurrences occur in up to 20-50% of patients. an immuno-mediated pathogenesis is ... | 2008 | 18708165 |
| confirmation by pcr of coxiella burnetii infection in animals at a zoo in lisbon, portugal. | | 2008 | 18708658 |
| q fever. | q fever is a worldwide zoonosis caused by the pathogen coxiella burnetii causing acute and chronic clinical manifestations. the name "q fever" derives from "query fever" and was given in 1935 following an outbreak of febrile illness in an abattoir in queensland, australia. c burnetii is considered a potential agent of bioterrorism (class b by the centers for disease control). | 2008 | 18755387 |
| [taxonometry, ecology and evolutional relation of rickettsia]. | ecology of rickettsia is main direction of researches of rams academician i. v. tarasevich and her team. characteristics of alpha 1-protobacteriae--parasites of eukaryotic cells--given in the work. the accent to rickettsia characteristics in relation of their evolution relationship with eukaryote mitochondria is made. main directions of researches in q-fever, tsutsugamushi fever, rickettsioses of tick-borne spotted fever (tsf) group, performed in our country under direction of rams academician i ... | 2008 | 18756777 |
| [ecological and epidemiological aspects of q-fever]. | ecological and epidemiological features of q-fever throughout its study are represented. the fact of transformation of q-fever natural focuses to antropurgical focuses is proved. it is established that intraherd focuses, determining epidemiological status of q-fever, have great persistence. significant economical loss due to recurrence and chronization of the infection are proved, economical loss from one case of the disease is calculated. | 2008 | 18756778 |
| [rams academician i. v. tarasevich is a leader of development of combined inactivated vaccine against q-fever]. | short information about significance of q-fever in human pathology is represented. necessity of vaccination is proved. the row of vaccines, developed in czechoslovakia and romania and identified as small-effective, was considered. live vaccine from m-44 strain, was made in ussr, still remains in russia. however, experimental data of american and russian authors showed persistency of q-fever agents in vaccinated animals, abortions and other pathology. who recommended declining to use live vaccine ... | 2008 | 18756779 |
| [medical geography in study of rickettsioses]. | shorts resume of medical-geographical studies of q-fever, tsutsugamushi fever, rickettsioses of tick-borne spotted fever is represented. influence of biotic and abiotic factors on spread and landscape tropism of infection agents is considered. types of focuses on endemic territories are determined, regions with high risk of infection for humans are detected. | 2008 | 18756781 |
| [nature-focal rickettsioses in mongolia. two decades of russian-mongolian scientific collaboration]. | the results of many years' studies of nature-focal rickettsioses in mongolia in collaboration of soviet (russian) and mongolian specialists under leadership of i. v. tarasevich are represented. presence of contacts of population, domestic and wild animals with coxiella burnetii and rickettsia sibirica on the territory of mongolia was established. the map of spread of ticks corresponding to their epidemiological significance was developed. methodological documents for prevention of nature-focal r ... | 2008 | 18756785 |
| prevalence of tick-borne zoonotic bacteria in questing adult ticks from northern spain. | a total of 691 questing adult ixodid ticks of the genera ixodes, haemaphysalis, dermacentor, and rhipicephalus were tested by polymerase chain reaction (pcr) and reverse line blot (rlb) for the presence of anaplasma phagocytophilum, coxiella burnetii, borrelia spp., and spotted fever group (sfg) rickettsiae. ticks were collected by blanket dragging during 2 sampling years (2003-2005) in 10 recreational areas in the basque country (northern spain). adult ticks were collected every month of the ye ... | 2008 | 18759563 |
| large ongoing q fever outbreak in the south of the netherlands, 2008. | | 2008 | 18761906 |
| the chlamydial inclusion preferentially intercepts basolaterally directed sphingomyelin-containing exocytic vacuoles. | chlamydiae replicate intracellularly within a unique vacuole termed the inclusion. the inclusion circumvents classical endosomal/lysosomal pathways but actively intercepts a subset of golgi-derived exocytic vesicles containing sphingomyelin (sm) and cholesterol. to further examine this interaction, we developed a polarized epithelial cell model to study vectoral trafficking of lipids and proteins to the inclusion. we examined seven epithelial cell lines for their ability to form single monolayer ... | 2008 | 18778406 |
| identification of protein candidates for the serodiagnosis of q fever endocarditis by an immunoproteomic approach. | q fever is a worldwide zoonosis caused by coxiella burnetii bacterium. two clinical forms are present: acute q fever and chronic disease, including endocarditis. currently, the diagnosis of q fever endocarditis is based on the detection of anti-phase i antibodies. the objective of the study was to identify candidate proteins for the serological diagnosis of endocarditis due to c. burnetii. the immunoreactivities of sera from 12 patients with c. burnetii infections, including the sera from patien ... | 2009 | 18797945 |
| q fever in the netherlands - what matters is seriousness of disease rather than quantity. | | 2008 | 18801314 |
| [q fever: a challenge for the veterinarian]. | | 2008 | 18807615 |
| adaptive immunity to the obligate intracellular pathogen coxiella burnetii. | coxiella burnetii is an obligate intracellular bacterial pathogen that causes the zoonosis q fever. while an effective whole-cell vaccine (wcv) against q fever exists, the vaccine has limitations in being highly reactogenic in sensitized individuals. thus, a safe and effective vaccine based on recombinant protein antigen (ag) is desirable. to achieve this goal, a better understanding of the host response to primary infection and the precise mechanisms involved in protective immunity to c. burnet ... | 2009 | 18813881 |
| prophylaxis after exposure to coxiella burnetii. | coxiella burnetii is a category b bioterrorism agent. we numerically evaluated the risks and benefits from postexposure prophylaxis (pep) after an intentional release of c. burnetii to the general population, pregnant women, and other high-risk populations. for each group, we constructed a decision tree to estimate illness and deaths averted by use of pep/100,000 population. we calculated the threshold points at which the number of pep-related adverse events was equal to the cases averted. pep w ... | 2008 | 18826819 |
| evaluation of the recombinant heat shock protein b (hspb) of coxiella burnetii as a potential antigen for immunodiagnostic of q fever in goats. | coxiella burnetii is an intracellular bacterium that causes a worldwide zoonosis, the q fever. currently, to diagnose the infection in ruminants, whole cell antigens-based elisas are used. in this study a heat shock protein, the hspb, was evaluated for its ability to be recognized by the goat immune system and its capacity to sign a stage of infection. the htpb gene of c. burnetii was cloned and sequenced. a high identity (>90%) was observed among the htpb genes of four ruminant strains tested. ... | 2009 | 18835111 |
| ovine-associated q fever. | in atlantic canada, the traditional risk factor for acquisition of q fever infection has been exposure to infected parturient cats or newborn kittens. in this study we describe the first case of q fever in nova scotia acquired as a result of direct exposure to sheep. a serosurvey of the associated flock was undertaken using an indirect immunofluorescence assay (ifa) testing for antibodies to phase i and phase ii coxiella burnetii antigens. this serosurvey revealed that 23 of 46 sheep (50%) were ... | 2009 | 18840318 |
| candidate antigens for q fever serodiagnosis revealed by immunoscreening of a coxiella burnetii protein microarray. | q fever is a widespread zoonosis caused by coxiella burnetii. diagnosis of q fever is usually based on serological testing of patient serum. the diagnostic antigen of test kits is formalin-fixed phase i and phase ii organisms of the nine mile reference strain. deficiencies of this antigen include (i) potential for cross-reactivity with other pathogens; (ii) an inability to distinguish between c. burnetii strains; and (iii) a need to propagate and purify c. burnetii, a difficult and potentially h ... | 2008 | 18845831 |
| seroprevalence of q fever (coxiellosis) in sheep from the southern marmara region, turkey. | little information is available in turkey on q fever, a zoonose caused by coxiella burnetii and transmitted from domestic ruminants. this study aimed at investigating the seroprevalence in sheep flocks from three provinces (bursa, balikesir and canakkale). serosurvey was undertaken on 42 flocks, which were categorised by sizes. sera were collected randomly from specific age groups within the young population. chekit q-fever elisa kit was used to identify the infection in sheep. the results showe ... | 2010 | 18848356 |
| [endocarditis due to coxiella burnetii (q fever): a rare or underdiagnosed disease? case report]. | q fever is a zoonosis of worldwide distribution that is caused by coxiella burnetii. however, reports of this disease in brazil are rare. seroepidemiological studies have shown relatively high frequencies of antibodies against coxiella burnetii in populations with occupational exposure. in humans, it can be manifested clinically as acute or chronic disease. endocarditis is the most frequent chronic form of q fever and the form with the greatest morbidity and mortality. we report a severe case of ... | 2008 | 18853017 |
| [not available]. | | 1948 | 18864219 |
| q fever. | | 1948 | 18869871 |
| q fever; present status in the united states. | | 1948 | 18872304 |
| experimental studies of q fever in cattle; observations on four heifers and two milk cows. | | 1948 | 18885745 |
| q fever studies in southern california; occurrence of coxiella burneti in the spinose ear tick, otobius megnini. | | 1948 | 18893251 |
| q fever. | | 1947 | 18897492 |
| q fever studies in southern california; recovery of rickettsia burneti from raw milk. | | 1948 | 18899804 |
| action of streptomycin in experimental infection with q fever. | | 1948 | 18907327 |
| a probable outbreak of q fever in munich, germany. | | 1947 | 18917279 |
| q fever notes. | | 1947 | 18919083 |
| q fever myocarditis. | clinical manifestations of q fever infection are fever, productive cough, decrease in exercise tolerance and chills. cardiovascular involvement is well recognized and usually presents as endocarditis and infection of an aneurysm or vascular graft. myocarditis has only rarely been described as a manifestation of acute q fever infection. in this report we describe a case of a young adult who presented with angina-like symptoms and ecg and biochemical markers indicative of acute coronary syndrome. ... | 2008 | 18923753 |
| artificial plasmid engineered to simulate multiple biological threat agents. | the objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. we identified and selected specific genes to detect yersinia pestis, francisella tularensis, burkholderia mallei, burkholderia pseudomallei, rickettsia sp., coxiella burnetii, brucella sp., enterohemorrhagic escherichia coli o157:h7, bacillus anthracis, and variola (smallpox) virus. we then designed a ... | 2009 | 18923830 |
| q fever in the netherlands: a concise overview and implications of the largest ongoing outbreak. | two outbreaks of q fever were reported in the netherlands in 2007 and 2008. the ongoing 2008 outbreak in the south-eastern part of the netherlands is the largest community outbreak ever described, with 808 cases reported until august 2008. the changing epidemiology of q fever is most likely related to intensive goat farming, and has important implications for the clinical care of patients in endemic areas. treatment of community-acquired pneumonia has to take possible q fever into account, and t ... | 2008 | 18931396 |
| cytokine polymorphisms have a synergistic effect on severity of the acute sickness response to infection. | functional polymorphisms in immune response genes are increasingly recognized as important contributors to the marked individual differences in susceptibility to and outcomes of infectious disease. the acute sickness response is a stereotypical set of illness manifestations mediated by the proinflammatory cytokines induced by many different pathogens. the genetic determinants of severity of the acute sickness response have not previously been explored. | 2008 | 18937577 |
| detection of coxiella burnetii in ticks collected from central spain. | a total of 1482 adult ticks collected from vegetation and animals in central spain in 2003-2005 were tested for the presence of coxiella burnetii by polymerase chain reaction and subsequent reverse line blot hybridization (pcr-rlb). c. burnetii was identified in 7.7% of questing ticks (80/1039) and 3.4% of ticks collected from animals (15/443) belonging to four species: hyalomma lusitanicum, dermacentor marginatus, rhiphicephalus sanguineus, and r. pusillus. these findings show an active role of ... | 2009 | 18945192 |
| acute q fever presenting as fever of unknown origin with rapidly progressive hepatic failure in a patient with alcoholism. | we report a case of fulminant acute q fever presenting as fever of unknown origin with rapidly progressive hepatic failure in a patient with alcoholism. a 51-year-old electrician, who was a habitual drinker, presented with a 2-week history of intermittent high fever, acute hepatomegaly and rapidly progressive jaundice after being accidentally exposed to dust from bird nests when he was repairing electrical equipment and circuitry at an abandoned factory in taipei county. ascites and prolonged pr ... | 2008 | 18971160 |
| ectoparasites and associated pathogens of free-roaming and captive animals in zoos of south carolina. | a survey of ectoparasites and their associated pathogens was conducted in two south carolina zoos, from 2004 to 2007. dead, wild birds and mammals, as well as captive animals examined during routine veterinary checks constituted the study populations. ectoparasites were tested for species of anaplasma, bartonella, coxiella burnetii, ehrlichia, rickettsia, and trypanosoma. forty-six species of ectoparasites were collected from 133 free-roaming and captive hosts and their associated nesting and be ... | 2009 | 18973443 |
| sustained activation of akt and erk1/2 is required for coxiella burnetii antiapoptotic activity. | coxiella burnetii is an obligate intracellular bacterial pathogen that directs biogenesis of a lysosome-like, parasitophorous vacuole in mammalian cells. we recently reported that c. burnetii inhibits apoptotic cell death in macrophages, presumably as a mechanism to sustain the host for completion of its lengthy infectious cycle. in the current study, we further investigated c. burnetii manipulation of host cell signaling and apoptosis by examining the effect of c. burnetii infection on activati ... | 2009 | 18981248 |
| coxiella burnetii shedding routes and antibody response after outbreaks of q fever-induced abortion in dairy goat herds. | q fever is a zoonosis caused by coxiella burnetii, a bacterium largely carried by ruminants and shed into milk, vaginal mucus, and feces. the main potential hazard to humans and animals is due to shedding of bacteria that can then persist in the environment and be aerosolized. the purpose of this study was to evaluate shedding after an outbreak of q fever abortion in goat herds and to assess the relationship with the occurrence of abortions and antibody responses. aborting and nonaborting goats ... | 2009 | 19011054 |
| [q fever: current diagnosis and treatment options]. | q fever is a zoonotic disease caused by the ubiquitous pathogen coxiella burnetii responsible for acute and chronic clinical manifestations. its geographically heterogeneous prevalence seems mainly related to the clinician interest and the availability of a reference center. its polymorphic clinical expression imposes reference to diagnosis in presence of pneumonia, hepatitis, prolonged fever or endocarditis with no proof of its etiology. the diagnosis is mainly serological. if acute q fever is ... | 2009 | 19013734 |
| brachial plexopathy associated with q fever: case report and review of the literature. | neurologic manifestations of q fever are predominantly central. we report the case of a 35-year-old man with recurrent fever and motor and sensory deficits in the right c5, c6 territories. electrophysiological findings were consistent with a right upper-trunk brachial plexopathy or with suprascapular and axillary neuropathies. the patient had full resolution of neurologic symptoms with antibiotic treatment. the association of brachial plexopathy with q fever infection, described in other rare in ... | 2008 | 19016536 |
| neospora caninum, potential cause of abortions in dairy cows: the current serological follow-up in slovakia. | neosporosis is considered to be a contributing risk factor for abortions in dairy cows and other farm animals and has negative economic impact on their breeding. in respect of the rapid spread of neosporosis in herds throughout the world, our aim was to detect the prevalence of anti-neospora antibodies in cows post-abortion (pa) (pa group n=716) and in cows without any reproduction problems (control group n=247) on large breeding farms from eastern slovakia. the overall mean seropositivity in pa ... | 2009 | 19019551 |
| [acute q fever, antiphopholipid antibodies and renal artery thrombosis: case report and literature review]. | antiphospholipid antibodies (apl) can be associated with numerous infectious and particularly q fever. data on the pathogenicity of apl in the course of acute q fever are scarce. | 2009 | 19026472 |
| spread of ticks and tick-borne diseases in germany due to global warming. | tick-transmitted diseases like tick-borne encephalitis and lyme borreliosis have been well known in germany for decades. global climate changes may influence the emergence and reemergence of diseases. ongoing research now gives an additional focus on other tick-borne pathogens such as coxiella burnetii, rickettsia conorii, anaplasma phagocytophilum and babesia spp., the causative agents of q-fever, mediterranean spotted fever, anaplasmosis and babesiosis, respectively. the epidemiology of these ... | 2008 | 19030892 |
| recurrent jarisch-herxheimer reaction in a patient with q fever pneumonia: a case report. | abstract: q fever is a zoonotic disease caused by coxiella burnetii. the jarisch-herxheimer reaction (jhr) is associated with the antibiotic treatment of certain bacterial infections. we report a very rare case of a 36-year-old male with q fever pneumonia that resulted in recurrent ards and presented the jhr during his treatment. the patient was admitted for treatment of community acquired pneumonia. he developed ards, was intubated and placed on mechanical ventilation. doxycycline was empirical ... | 2008 | 19040755 |
| comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus coxiella. | genetically distinct isolates of coxiella burnetii, the cause of human q fever, display different phenotypes with respect to in vitro infectivity/cytopathology and pathogenicity for laboratory animals. moreover, correlations between c. burnetii genomic groups and human disease presentation (acute versus chronic) have been described, suggesting that isolates have distinct virulence characteristics. to provide a more-complete understanding of c. burnetii's genetic diversity, evolution, and pathoge ... | 2009 | 19047403 |
| role of the lone star tick, amblyomma americanum (l.), in human and animal diseases. | we reviewed scientific literature pertaining to known and putative disease agents associated with the lone star tick, amblyomma americanum. reports in the literature concerning the role of the lone star tick in the transmission of pathogens of human and animal diseases have sometimes been unclear and even contradictory. this overview has indicated that a. americanum is involved in the ecology of several disease agents of humans and other animals, and the role of this tick as a vector of these di ... | 2009 | 19054615 |
| multiplexed whole bacterial antigen microarray, a new format for the automation of serodiagnosis: the culture-negative endocarditis paradigm. | the serological diagnosis of blood culture-negative endocarditis due to coxiella burnetii, bartonella spp., brucella melitensis and legionella pneumophila is based on a manual immunofluorescence assay (ifa), which is taken to be the reference method. the automated ifa inodiag multiplexed antigenic microarray, which includes a slide with all the above bacteria and four internal controls, an incubator, a fluorescent reader and software with an algorithm of interpretation for infectious endocarditi ... | 2008 | 19076842 |
| comparison of the new inodiag automated fluorescence multiplexed antigen microarray to the reference technique in the serodiagnosis of atypical bacterial pneumonia. | the aetiological diagnosis of pneumonia depends largely on culture-, antigen- or pcr-based tests. atypical agents of pneumonia include coxiella burnetii, chlamydophila pneumoniae, chlamydia psittaci, legionella pneumophila, francisella tularensis and mycoplasma pneumoniae. in these cases, serological tests are commonly used for diagnosis. all of the above species were comparatively screened for by using the inodiag multiplexed automatic immunofluorescence assay and established reference techniqu ... | 2008 | 19076843 |
| [aortic aneurysm and coxiella burnetii]. | | 2008 | 19094873 |
| [acute acalculous cholecystitis due to coxiella burnetii]. | | 2008 | 19094886 |
| acute q-fever and history taking--a lesson learned. | a 50-year-old patient underwent a routine primary total hip replacement. soon after surgery, he developed acute respiratory failure from post-operative sepsis. his condition deteriorated rapidly despite supportive management and he required admission into intensive care unit for assisted ventilation. it took almost one week before the underlying cause of the deterioration was determined to be unrelated to complications of surgery. a diagnosis of q-fever was made following detailed attention to t ... | 2008 | 19097013 |
| prevalence of q fever in domestic animals with reproductive disorders. | the occurrence of coxiella burnetii in animals with reproductive disorders was studied. a total of 920 samples (genital and faecal swabs, milk, urine and serum) were collected from cows (88), buffaloes (33), sheep (43) and goats (53) with a history of reproductive disorders and screened for c. burnetii by a pcr assay targeting the repetitive transposon-like region of c. burnetii (trans-pcr), real-time pcr, indirect immunofluorescence assay (ifa), enzyme-linked immunosorbent assay (elisa) and iso ... | 2010 | 19101035 |
| systemic immune presentations of coxiella burnetii infection (q fever). | q fever is a worldwide zoonosis caused by coxiella burnetii. its presentation can be atypical, delaying and complicating the diagnosis. we report 7 cases of q fever mimicking vasculitis, systemic inflammatory disease, or auto-immune disorder. | 2010 | 19110298 |
| characterization of a coxiella burnetii ftsz mutant generated by himar1 transposon mutagenesis. | coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human q fever. the lack of methods to genetically manipulate c. burnetii significantly impedes the study of this organism. we describe here the cloning and characterization of a c. burnetii ftsz mutant generated by mariner-based himar1 transposon (tn) mutagenesis. c. burnetii was coelectroporated with a plasmid encoding the himar1 c9 transposase variant and a plasmid containing a himar1 transposon en ... | 2009 | 19114492 |
| field collection and genetic classification of tick-borne rickettsiae and rickettsiae-like pathogens from south texas: coxiella burnetii isolated from field-collected amblyomma cajennense. | we are reporting the first known isolation of the q-fever agent coxiella burnetii from field-collected cayenne ticks amblyomma cajennense in north america. q-fever affects a number of domestic ungulates where it can lead to abortion in sheep and goats. there is far less known about the disease's effects on wild species, primarily because of the tendency of the disease to self resolve and to provide long-term immunity to subsequent infections. the first recovery of c. burnetii in north america wa ... | 2008 | 19120212 |