| gnrh peptides and regulation of the corpus luteum. | | 1989 | 2681738 |
| mechanism of action of gnrh-induced oocyte maturation. | | 1989 | 2681744 |
| comparison of direct immunofluorescent staining and an enzyme-linked immunoabsorbent assay for identifying chlamydia trachomatis in cervical and urethral specimens. | a direct fluorescent antibody (dfa) stain (microtrak, syva) and an enzyme-linked immunoabsorbent assay (chlamydiazyme, abbott) were used to detect chlamydial infection in 179 cervical specimens and 56 specimens obtained from the female urethra. chlamydiazyme, when compared with the dfa technique, revealed a high degree of correlation in these specimens (22 and 21 positive cervical specimens, respectively, and 7 each positive urethral specimens). since both techniques are readily available, inexp ... | 1989 | 2681749 |
| the failure of intravenous cyclophosphamide therapy in refractory idiopathic inflammatory myopathy. | eleven patients with idiopathic inflammatory myopathy refractory to treatment with corticosteroids and other immunosuppressive agents were treated with monthly intravenous cyclophosphamide (0.75-1.357 g/m2). six patients completed a full course of 7 infusions at which point only one patient met predefined criteria for improvement in both strength and function. five had modest improvement in strength but did not meet the criteria for improvement. all patients have subsequently required treatment ... | 1989 | 2681763 |
| acute gluteal abscesses: injectable chloroquine as a cause. | one hundred and seven patients admitted to the casualty department with acute gluteal abscesses and surgically drained were studied. in 91 patients (85%) the abscess was a result of an intramuscular injection, of whom 71 patients (66%) had the abscess following a chloroquine injection (antimalarial). of those patients with an abscess following an intramuscular injection, in 64 patients (70%) a disposable syringe was used for injection of the drug and in 18% a boiled disposable was used. the inje ... | 1989 | 2681814 |
| [reca-independence of the process of amplification caused by the rs-1 sequence of vibrio cholerae]. | the processes of amplification and deamplification of a plasmid dna segment flanked by direct repeats of rsi-sequence of vibrio cholerae and carrying the structural genes of cholera toxin inside the recombinant plasmid in e. coli cells have been studied. these processes determined by rsi-sequences are shown to take place independent of the reca-system of e. coli cells. | 1989 | 2682220 |
| vibrio cholerae non-o1 infection presenting as localized cellulitis. | vibrio cholerae non-o1 characteristically causes gastrointestinal illness but can occasionally be responsible for extra-intestinal infections. the authors report an unusual case of a man presenting with extensive cellulitis that did not improve with conventional antimicrobial therapy. vibrio cholerae non-o1 was later isolated from a cutaneous bulla. although he had severe hepatic cirrhosis, the infection remained confined to the cutaneous tissues and he was never bacteremic. he improved dramatic ... | 1989 | 2683771 |
| single-dose treatment of cholera with furazolidone or tetracycline in a double-blind randomized trial. | to evaluate single doses of 400 mg of furazolidone and 1 g of tetracycline given orally to patients with diarrhea due to vibrio cholerae, we studied 87 adults in a randomized, double-blind, placebo-controlled trial. all patients received intravenous fluids for rehydration and no other drugs. the total volumes of stool (mean +/- standard deviation) during a 6-day period after treatment were significantly smaller in the tetracycline group (10.5 +/- 8.6 liters) than in the furazolidone group (20.9 ... | 1989 | 2684006 |
| detection with synthetic oligonucleotide probes of nucleotide sequence variations in the genes encoding enterotoxins of escherichia coli. | we examined variations in the genes encoding heat-stable enterotoxin (st) and heat-labile enterotoxin (lt) in 88 strains of escherichia coli isolated from individuals with traveler's diarrhea to find suitable sequences for use as oligonucleotide probes. four oligonucleotide probes of the gene encoding st of human origin (stib or sth), one oligonucleotide probe of the gene encoding st of porcine origin (stia or stp), and three oligonucleotide probes of the gene encoding lt of human origin (ltih) ... | 1989 | 2685027 |
| [clinical significance of immunoscintigraphy for the diagnosis and treatment of gastrointestinal malignant tumors]. | eighty-one immunoscintigraphy (is) studies in 80 patients with gastrointestinal malignancies were re-evaluated retrospectively to analyze accuracy and clinical impact of the is findings. selection of patients was based upon complete diagnostic records and a clinical follow-up of at least 6 months. the 3 antibody preparations used (99m-tc labelled complete anti-cea antibodies (bw 431/26), 111-in labelled f(ab')2-fragments against cea (bw 431/31), and a mixture of 131-i labelled f(ab')2-fragments ... | 1989 | 2685690 |
| [the development of a quantitative method for the in vivo determination of the adhesive activity of vibrio cholerae]. | the dynamics of the distribution and adhesion of v. cholerae in the intestine of suckling rabbits has been studied. the quantitative method for the in vivo determination of the adhesive activity of v. cholerae has been developed with the use of suckling rabbits as an experimental model. the method may be used for the determination of v. cholerae virulence and the pathogenesis of cholera. | 1989 | 2686301 |
| [quantitative evaluation of the adhesive activity of vibrio cholerae with various degrees of virulence]. | in the studies on the evaluation of v. cholerae adhesion on suckling rabbits by our method, the adhesive activity of the vibrios has been found to be directly related to their virulence. this method may be used as an additional test for the differentiation of virulent and avirulent v. cholerae. | 1989 | 2686311 |
| mechanisms of resistance in anaerobes and new developments in testing. | anaerobic bacteria currently demonstrate increased resistance to antimicrobial agents, primarily by the production of beta-lactamase. a number of species of bacteroides, most notably those in the bacteroides fragilis group, produce these enzymes. a few species of fusobacterium and clostridium produce beta-lactamase as well. fortunately, this mechanism of resistance is readily overcome by administering beta-lactamase inhibitors coupled with a beta-lactam antibiotic that would otherwise be inactiv ... | 1989 | 2686914 |
| methyl-directed repair of frameshift heteroduplexes in cell extracts from escherichia coli. | the methyl-directed dna repair efficiency of a series of m13mp9 frameshift heteroduplexes 1, 2, or 3 unpaired bases was determined by using an in vitro dna mismatch repair assay. repair of hemimethylated frameshift heteroduplexes in vitro was directed to the unmethylated strand; was dependent on muth, mutl, and muts; and was equally efficient on base insertions and deletions. however, fully methylated frameshift heteroduplexes were resistant to repair, while totally unmethylated substrates were ... | 1989 | 2687237 |
| regulator and enzyme specificities of the tol plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway. | the tol plasmid upper pathway operon encodes enzymes involved in the catabolism of aromatic hydrocarbons such as toluene and xylenes. the regulator of the gene pathway, the xylr protein, exhibits a very broad effector specificity, being able to recognize as effectors not only pathway substrates but also a wide variety of mono- and disubstituted methyl-, ethyl-, and chlorotoluenes, benzyl alcohols, and p-chlorobenzaldehyde. benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase, two upper pa ... | 1989 | 2687253 |
| sodium-transport nadh-quinone reductase of a marine vibrio alginolyticus. | the respiratory chain of a marine bacterium, vibrio alginolyticus, required na+ for maximum activity, and the site of na+ -dependent activation was localized on the nadh-quinone reductase segment. the na+ -dependent nadh-quinone reductase extruded na+ as a direct result of redox reaction. it was composed of three subunits, alpha, beta, and gamma, with apparent mr of 52, 46, and 32 kda, respectively. the reduction of ubiquinone-1 to ubiquinol proceeded via ubisemiquinone radicals. the former reac ... | 1989 | 2687259 |
| expression of the catalytic subunit of camp-dependent protein kinase in escherichia coli. | a cdna clone for the catalytic subunit of murine camp-dependent protein kinase was placed into two expression vectors, plws-3 and plsw-4. for plws-3, the entire coding region of the catalytic subunit was inserted into the ndei site of pt7-7 under the control of the t7 promoter. plws-4 contains a polycistronic transcript under control of the lac uv5 promoter encoding for the type i regulatory subunit followed by the catalytic subunit. significant expression was achieved with plws-4 in escherichia ... | 1989 | 2687267 |
| complement component c1q enhances invasion of human mononuclear phagocytes and fibroblasts by trypanosoma cruzi trypomastigotes. | internalization and infectivity of trypanosoma cruzi trypomastigotes by macrophages is enhanced by prior treatment of parasites with normal human serum. heating serum or removing c1q from serum abrogates the enhancement, but augmentation of attachment and infectivity is restored by addition of purified c1q to either serum source. although both noninfective epimastigotes (epi) and vertebrate-stage tissue culture trypomastigotes (tct) bind c1q in saturable fashion at 4 degrees c, internalization b ... | 1989 | 2687332 |
| a mutation in the dam gene of vibrio cholerae: 2-aminopurine sensitivity with intact gatc methylase activity. | vibrio cholerae mutants sensitive to 2-aminopurine (2ap) but with dna adenine methylase activity similar to parental cells have been isolated. the mutant strains were sensitive to ultraviolet light (uv), methyl methane sulphonate (mms) and 9-aminoacridine. the spontaneous mutation frequency of the mutants were not significantly affected. attempts to isolate dam v. cholerae cells by screening 2ap sensitive cells have not been successful. all the mutant phenotypes could be suppressed by introducin ... | 1989 | 2688642 |
| assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin. | a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ... | 1989 | 2688936 |
| cholera on the louisiana gulf coast: historical notes and case report. | a 67-year old woman was brought by ambulance to the hospital because of syncope and collapse. forty-eight hours prior to the admission she ate some crabs with her husband. the morning of admission she awakened with massive, watery diarrhea followed by vomiting and shock. she was admitted to the intensive care unit with hypotension and bradycardia. she was resuscitated after a large volume of fluid was administered. approximately 22 liters of fluids were administered in 24 hours. stool cultures g ... | 1989 | 2689545 |
| antibiotic resistance in the tropics. 1. the genetics of bacterial ampicillin resistance in tropical areas. | ampicillin and its derivatives are the most widely used beta-lactam antibiotics throughout the world. ampicillin resistance in gram-negative bacteria is usually manifested by plasmid-encoded beta-lactamases, which hydrolyse the beta-lactam ring of the antibiotic. there are at least 30 different plasmid-encoded beta-lactamases but almost all of them are found very infrequently. the one exception is the tem-1 beta-lactamase which is found wherever transferable ampicillin resistance emerges and acc ... | 1989 | 2690414 |
| characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan. | the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ... | 1989 | 2690736 |
| [principle, method, and application for the production of peptide hormones using genetic engineering]. | | 1989 | 2691727 |
| exercise training produces changes in free and conjugated catecholamines. | in dogs the concentrations of conjugated dopamine in plasma have previously been shown to increase after exercise training. this study was done to determine whether conjugated norepinephrine and epinephrine also increase. fifteen dogs were randomly divided into training (n = 8) or sedentary (n = 7) groups. all dogs were exercised acutely for 5 min at 4 mph with a 12% grade, following a 3-min warm-up, before and after either a 12-wk training or a 12-wk sedentary period. free and conjugated catech ... | 1989 | 2691817 |
| ontogeny and regulation of drug metabolism in humans. phase i: monooxygenases. | most data concerning the development and regulation of cytochrome p-450-dependent monooxygenases were obtained in experimental animals, especially rats, by two successive approaches: first, the assay of total liver cytochrome p-450 and of related monooxygenase activities; then, more recently, the immunoquantification of molecular forms of cytochrome p-450, and the estimation of corresponding mrnas with nucleic acid probes. results were compared, before and after birth, in untreated animals, and ... | 1989 | 2693005 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| [various approaches to studying molecular-genetic mechanisms of low-temperature adaptation of microbial populations]. | the effect of biologically low temperature (12 degrees c) on the parameters of microbial population such as survival, catalase activity and its isoenzyme spectrum have been investigated on the models of escherichia coli and yersinia pseudotuberculosis. the quantity of nucleic acids, plasmid spectrum and temperature effect on the level of plasmid dna spiralization were studied under these conditions. the process of molecular genetic adaptation of bacterial populations having the broad temperature ... | 1989 | 2693957 |
| improved representation of cell surface structures by freeze substitution and backscattered electron imaging. | a surface preparation method for the sem based on cryofixation is presented suitable for the demonstration of membrane particles on whole cells. llc-pk1 cells (a renal epithelial cell line in culture) were fast frozen, freeze substituted, critical point dried, shadowed with 2 nm of platinum carbon and stabilized with a carbon backing layer. membrane bound particles are visualized by the material dependent backscattered electron image mainly originating from the platinum shadow. the surface of th ... | 1989 | 2694269 |
| mechanism of lhrh-stimulated steroidogenesis in rat leydig cells: lipoxygenase products of arachidonic acid may not be involved. | luteinizing hormone releasing hormone agonist, [(imbzl)-dhis6,pro9,net]-lhrh (lhrh-a), caused a two to threefold increase in in vitro testosterone (t) secretion by rat leydig cells. this lhrh-a-induced t secretion was completely blocked by quinacrine and chloroquine, inhibitors of phospholipase a2. addition of phospholipase a2, however, was ineffective in stimulating basal or lhrh-a-induced t secretion. phospholipase c, on the other hand, significantly stimulated both basal and lhrh-a-induced t ... | 1989 | 2695506 |
| chemotaxonomic study of vibrio cholerae bio-serogroup hakata on the basis of the sugar composition of the polysaccharide portion of its lipopolysaccharides. | a chemotaxonomic study was carried out on 31 strains of non-o1 vibrio cholerae bio-serogroup hakata, isolated in japan, which possesses the inaba antigen c of o1 v. cholerae. on the basis of the compositional sugar pattern of the polysaccharide portion of their lipopolysaccharides, the 23 strains isolated from the environment were separated into two groups, one (20 strains) containing mannose, glucose, fructose, l-glycero-d-mannoheptose, glucosamine, perosamine, quinovosamine, and an unidentifie ... | 1989 | 2695790 |
| the role of eicosanoids in human glomerular disease. | | 1989 | 2696358 |
| the role of eicosanoids in human glomerular disease. | | 1989 | 2696358 |
| group a streptococci revisited. | | 1989 | 2698123 |
| [human tympanic membrane temperature]. | | 1989 | 2698419 |
| [hiv infection--a sexually transmitted disease]. | in the light of literature reports and own experiences and observations the most important epidemiological aspects of hiv infection acquired through sexual contacts are discussed, including the likelihood of infection during heterosexual or homosexual intercourse, factors increasing the infection risk in homosexuals intercourse and the more or less safe forms of sexual intercourse. the combination of hiv infections with infections with other sexually transmitted diseases is discussed on the basi ... | 1989 | 2698486 |
| proteins of the inner and outer centromere of mitotic chromosomes. | we have used immunocytochemistry and molecular cloning methods to identify and characterize structural polypeptides of the centromere. these studies permit us to resolve two distinct regions: the inner and outer centromere. (i) components of the outer centromere: autoantibodies from certain patients with rheumatic disease identify a family of three immunologically related polypeptides that we have designated cenp-a (17 kda), cenp-b (80 kda), and cenp-c (140 kda). cenp-b has been cloned and seque ... | 1989 | 2698830 |
| [study of coenzyme reorientation in the active center of tryptophanase by linear dichroism method]. | tryptophanase from e.coli was oriented in a compressed slab of polyacrylamide gel and its linear dichroism (ld) and absorption spectra were measured. the free enzyme displays four ld bands at 305, 340, 425 and 490 nm. two bands at 340 and 425 nm belong to the internal coenzyme-lysine aldimine. the 305 nm band apparently belongs to an aromatic amino acid residue; the sign and form of this band are changed upon the enzyme reaction with substrate analogs. the 490 nm band is present in the ld spectr ... | 1989 | 2698994 |
| [pharmacokinetics of chinoin-170 in rats and dogs]. | | 1989 | 2701628 |
| [pharmacokinetics of chinoin-170 in rats and dogs]. | | 1989 | 2701628 |
| effect of accelerated natural lactic fermentation of infant food ingredients on some pathogenic microorganisms. | accelerated natural lactic fermentation in mixtures of water and ground sorghum, millet and pigeon pea was obtained by gradual selection of lactic acid bacteria, through inoculum recycling. weaning food prepared from ingredients fermented this way, contained approx. 0.7% lactic and 0.05% acetic acids and had a ph of about 3.8. in porridges, a ph of less than or equal to 4.0 was required to cause death of salmonella typhimurium and staphylococcus aureus. several intestinal pathogenic bacteria wer ... | 1989 | 2701696 |
| the immunological consequences of feeding cholera toxin. i. feeding cholera toxin suppresses the induction of systemic delayed-type hypersensitivity but not humoral immunity. | immunization of adult balb/c mice with 1 microgram cholera toxin (ct) in complete freund's adjuvant (cfa) induced both humoral (igg and iga) and cell-mediated (dth) immunity. although an immunopurified, formalinized, cholera toxoid (td) in cfa was inferior to the native holotoxin at inducing antitoxin antibodies, both cholera-derived antigens were equally immunogenic for specific dth. when mice were fed either 1 microgram ct or 5 microgram td 1 week before immunization, the induction of dth was ... | 1989 | 2703255 |
| depression of nonadaptive immunity after surgical stress: influence on metastatic spread. | the question of increased tumor cell dissemination after surgical stress was addressed in the model system of the spontaneously metastasizing rat adenocarcinoma bsp73asml, wherein amputation of the hind leg 7 days after intrafootpad implantation of tumor cells cured the animals, while surgical stress by laparotomy 2 days prior to amputation resulted in lung metastases in 80% of rats. a detailed in vitro analysis of natural killer (nk) and macrophage (mo) activity in different lymphatic compartme ... | 1989 | 2703300 |
| depression of nonadaptive immunity after surgical stress: influence on metastatic spread. | the question of increased tumor cell dissemination after surgical stress was addressed in the model system of the spontaneously metastasizing rat adenocarcinoma bsp73asml, wherein amputation of the hind leg 7 days after intrafootpad implantation of tumor cells cured the animals, while surgical stress by laparotomy 2 days prior to amputation resulted in lung metastases in 80% of rats. a detailed in vitro analysis of natural killer (nk) and macrophage (mo) activity in different lymphatic compartme ... | 1989 | 2703300 |
| influence of phospholipids on ruminal fermentation in vitro and on nutrient digestion and serum lipids in sheep. | phospholipid supplements were incubated in vitro or fed to sheep to determine how they affected ruminal fermentation, nutrient digestion and serum lipid concentration. in vitro, deoiled soybean lecithin added to hay increased (p less than .05) total vfa concentration but had no effect on fiber digestion in four of five trials. purified phospholipid (synthetic phosphatidylethanolamine), however, had little effect on in vitro hay fermentation when tested at four levels (0, 10, 20 or 30 mg) in thre ... | 1989 | 2703447 |
| dna strand break accumulation, thymidylate synthesis and nad levels in lymphocytes from methyl donor-deficient rats. | although tumor development with methyl donor-deficient diets has been well established, the biochemical and molecular mechanisms which predispose to tumor development are not yet understood. in the present study, dna damage and nucleotide metabolism associated with dna synthesis and repair were evaluated in splenic lymphocytes from rats fed a basal diet low in methionine and lacking in choline and folate or a supplemented control diet for a period of 3 wk. the accumulation of dna strand breaks, ... | 1989 | 2703920 |
| aminopyridines enhance opening of calcium-activated potassium channels in gh3 anterior pituitary cells. | the effects of aminopyridine analogs on ca2+-activated k+ channels in gh3 clonal anterior pituitary cells were studied using whole-cell voltage-clamp and single-channel recording techniques. step depolarization from a holding potential of -50 mv activated a noninactivating, tetraethylammonium- and cd2+-sensitive outward current. tail current analysis indicated that this sustained outward current is carried predominantly by k+ ions. extracellular perfusion with 4-aminopyridine and 3,4-diaminopyri ... | 1989 | 2704369 |
| estimation of the avidity of antibodies in polyclonal antisera against streptococcus pneumoniae type 3 by inhibition elisa. | the reliability of the determination of antibody avidity in polyclonal sera by indirect sandwich elisa was studied. binding of igm and igg (sub)classes in unpurified serum to streptococcus pneumoniae type 3 capsular polysaccharide, which was coated onto elisa plates, was inhibited with different inhibitors. the inhibitor concn at which 50% inhibition of antibody binding to the elisa coat was achieved, was used as a measure for antibody avidity. as this 50% inhibition value is dependent upon the ... | 1989 | 2704374 |
| effects of undernutrition on infection with vibrio cholerae o1 and on response to oral cholera vaccine. | the association between undernutrition and the risk of colonization and disease with vibrio cholerae o1, concentrations of salivary iga and the serologic response to infection and to orally administered cholera b subunit were examined prospectively in a family study in bangladesh. children ages 1 to 8 years who were family contacts of patients hospitalized with culture-confirmed cholera were visited within 24 hours of the hospitalization and daily for 10 days, queried for the presence of diarrhe ... | 1989 | 2704600 |
| cell cycle traverse in nhik-3025 carcinoma of the uterine cervix after low-dose-rate irradiation. | theoretically, fractionation schemes could be tailored to the individual pattern of radiation-induced synchronization of cells in the radiosensitive g2 phase, leading to more effective radiotherapy. using a human cervical carcinoma xenografted to nude mice, the effects of low-dose-rate irradiation on the cell cycle distribution were studied. flow cytometric analysis demonstrated that cells accumulated in g2 + m phase 35 h after a total dose of 10 gy of 137cs irradiation. this accumulation time c ... | 1989 | 2704783 |
| streptococcus pneumoniae endocarditis presenting as an epidural abscess. | streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ... | 1989 | 2704929 |
| streptococcus pneumoniae endocarditis presenting as an epidural abscess. | streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ... | 1989 | 2704929 |
| isolation of vibrio cholerae from aquatic birds in colorado and utah. | vibrio cholerae was isolated from cloacal swabs and freshly voided feces collected from 20 species of aquatic birds in colorado and utah during 1986 and 1987. about 17% (198 of 1,131) fecal specimens collected from july 1986 through august 1987 contained the organism. both o1 and non-o1 v. cholerae strains were isolated from the fecal specimens. isolates from eight birds (representing five species) agglutinated in o group 1 antiserum. supernatants of broth cultures from three isolates which type ... | 1989 | 2705773 |
| post-training memory facilitation blocks extinction but not retroactive interference. | rats were trained in a step-down inhibitory avoidance task using a 0.2-ma footshock and tested 6 h later. exposure to a session of extinction (animals placed on the box where they had been trained and left to explore it freely for 100.0 s without footshocks) or to a series of 10 tones presented in another box, in the dark, 2 h after training, hindered retention test performance. the immediate post-training ip administration of epinephrine (5.0 micrograms/kg), acth-(1-24) (0.2 microgram/kg), or l ... | 1989 | 2705977 |
| post-training memory facilitation blocks extinction but not retroactive interference. | rats were trained in a step-down inhibitory avoidance task using a 0.2-ma footshock and tested 6 h later. exposure to a session of extinction (animals placed on the box where they had been trained and left to explore it freely for 100.0 s without footshocks) or to a series of 10 tones presented in another box, in the dark, 2 h after training, hindered retention test performance. the immediate post-training ip administration of epinephrine (5.0 micrograms/kg), acth-(1-24) (0.2 microgram/kg), or l ... | 1989 | 2705977 |
| involvement of protein kinase c in the ca2+-dependent vesicular release of gaba from central and enteric neurons of the guinea pig. | the involvement of protein kinase c (pkc) in the release of endogenous gamma-aminobutyric acid (gaba) was studied using slices of deep cerebellar nucleus and strips of small intestine from the guinea pig. 12-o-tetradecanoylphorbol 13-acetate (tpa), but not 4 alpha-phorbol-12,13-didecanoate (4 alpha-pdd), potentiated the high k+-evoked release of gaba from both preparations in the presence of tetrodotoxin. ouabain evoked the release of gaba from both preparations, and this release was not altered ... | 1989 | 2706529 |
| factors affecting the response of lung clearance systems to acid aerosols: role of exposure concentration, exposure time, and relative acidity. | the ability of the lungs to clear deposited material is essential for maintenance of lung homeostasis. acid aerosols have been shown to alter the efficiency of this process. this paper assesses the role of acid aerosol exposure concentration (c), exposure time (t), and relative acidity in producing changes in clearance from both the tracheobronchial tree and respiratory region of the lungs of rabbits. the response was found to be due to total exposure, i.e., some combination form of c x t, and w ... | 1989 | 2707191 |
| fully automated capillary isotachophoresis of proteins. | an apparatus for fully automated capillary isotachophoresis was constructed. a commercial apparatus (shimadzu ip-2a) was modified in the electrolyte pumping system and the flow lines were simplified. an automatic sampler was used for sequential sampling. the equipment, pumps, the sampler, a high-voltage dc power supply, and a recorder, were controlled by a system controller which comprises a microcomputer and a basic program for time-control of the equipments. the apparatus was successfully used ... | 1989 | 2707241 |
| increased hepatic lipid peroxidation with methionine toxicity in the rat. | consumption of excess methionine by rats is known to cause membrane damage, liver enlargement and accumulation of iron in the spleen. in this study two groups (n = 5) of male, wistar rats were pair-fed either a methionine supplemented (20.0 g/kg) or control (2.0 g/kg) diet for 7 weeks. hepatic and erythrocyte copper-zinc superoxide dismutase activities were significantly reduced (p less than 0.05 and p less than 0.001 respectively) by methionine supplementation while the activities of catalase ( ... | 1989 | 2707623 |
| neurogenesis of glutamic acid decarboxylase immunoreactive cells in the hippocampus of the mouse. i: regio superior and regio inferior. | the neurogenetic gradients of neurons showing glutamic acid decarboxylase (gad) immunoreactivity were determined in the regio superior and in the regio inferior of the mouse hippocampus. pregnant c57bl mice received pulse injections of (3h)thymidine from e11 through e17 (e0 being the day of mating). distributions of (3h)thymidine-labeled, gad-positive neurons in the different strata of the hippocampus proper were recorded in adult animals. gad-positive neurons in this region are generated prenat ... | 1989 | 2708583 |
| establishment of the experimental conditions for inducing saccular cerebral aneurysms in primates with special reference to hypertension. | to find out the optimum experimental conditions for the induction of saccular cerebral aneurysms in primates, various procedures for inducing renal hypertension were used in cynomolgus monkeys treated with ligation of unilateral carotid artery and beta-amino-propionitrile feeding. animals were divided into four groups. in the first group, both kidneys were simultaneously compressed with a figure-eight ligation. in the second group, the kidneys were ligated at an interval of one week. in the thir ... | 1989 | 2711898 |
| an attempt to evaluate diagnostic and prognostic significance of blood endogenous ethanol in alcoholics and their relatives. | endogenous ethanol in the blood of human subjects was measured by gas chromatography. in healthy males, 12-13-year-old boys (sons of alcoholic and nonalcoholic fathers), and alcoholic inpatients (after cessation of all drugs), the endogenous ethanol levels ranged from 0 to 4.3 mg/l. the results showed no significant differences between the groups. at the period of alcohol withdrawal reactions the concentrations of endogenous ethanol were minimal in patients with delirium tremens and maximal in p ... | 1989 | 2713093 |
| periosteal neochondrogenesis for biologically resurfacing joints: its cellular origin. | the purpose of this study was to determine if the hyaline-like cartilage produced in major full-thickness defects of a joint surface, treated by a free periosteal graft and subjected to continuous passive motion (cpm), originated exclusively from the progenitor cells of the cambium of the graft. free periosteal grafts were raised from the tibia of both hind legs of eight male new zealand rabbits and transplanted into full-thickness defects across the entire width of the patellar groove of 15 fem ... | 1989 | 2713771 |
| periosteal neochondrogenesis for biologically resurfacing joints: its cellular origin. | the purpose of this study was to determine if the hyaline-like cartilage produced in major full-thickness defects of a joint surface, treated by a free periosteal graft and subjected to continuous passive motion (cpm), originated exclusively from the progenitor cells of the cambium of the graft. free periosteal grafts were raised from the tibia of both hind legs of eight male new zealand rabbits and transplanted into full-thickness defects across the entire width of the patellar groove of 15 fem ... | 1989 | 2713771 |
| examination, by 1h-n.m.r. spectroscopy, of the binding of a synthetic, high-affinity heparin pentasaccharide to human antithrombin iii. | binding of a synthetic, high-affinity heparin pentasaccharide and of intact heparin to both native and elastase-modified human antithrombin iii have been examined by 1h-n.m.r. spectroscopy. the pentasaccharide perturbs many protein resonances in the same way as does intact heparin. there are, however, differences that seem to arise both from fewer contacts in the heparin binding-site when the pentasaccharide binds and from dissimilar conformational changes in the protein. the resonance of the h- ... | 1989 | 2713873 |
| effect of estradiol on the phosphorylation of rat liver ribosome proteins. | the effect of estradiol on phosphorylation with gamma-32p-atp of ribosome proteins from polyribosome fraction, isolated from female rat liver is found out. rats (180 g) used were: sham-operated, ovariectomized and treated after ovariectomy for 11 days with 20 micrograms 17 beta-estradiol-dipropionate per 100 g body weight. the estradiol deficiency after ovariectomy decreased phosphorylation, but the estrogenization of ovariectomized rats increases it twice. in vitro the hormone (1-2 mumol/l) [co ... | 1989 | 2714224 |
| effects of inducers of differentiation on protein kinase c and cmp-n-acetylneuraminic acid:lactosylceramide sialyltransferase activities of hl-60 leukemia cells. | exposure of hl-60 leukemia cells to either 12-o-tetradecanoylphorbol-13-acetate (tpa), dimethylsulfoxide (dmso), exogenous gangliosides gm3, gm1, or bovine brain ganglioside mixture (bbg) resulted in a marked inhibition of the growth of cells. the order of the inhibitory potency was tpa greater than gm3 greater than dmso greater than bbg greater than gm1. in contrast, sulfatides were without effect on cellular replication. treatment of hl-60 cells with tpa or gm3 induced differentiation along th ... | 1989 | 2715723 |
| tumor necrosis factor enhances expression of tissue factor mrna in endothelial cells. | we have examined the effect of recombinant tumor necrosis factor on the expression of tissue factor activity and tissue factor mrna levels in vascular endothelial cells. following exposure of human umbilical vein endothelial cells to this cytokine, the appearance of tissue factor procoagulant activity was detected following cell disruption, and was maximal at 6 hours. northern blot analysis of cytokine-treated cells demonstrated a similar increase in the synthesis of tissue factor mrna, followed ... | 1989 | 2718149 |
| survival and weight increase in the fetal rat after selective hypophysectomy in utero. | rat fetuses were subjected to selective hypophysectomy in utero on day 16, 17 or 18 of gestation and inspected on day 22 (the day before birth). the survival rate of fetuses operated on day 17 and 18 was about 20 and 50%, respectively. losses were mostly caused by the surgery preceding the removal of the pituitary, and no significant survival-promoting activity of the gland could be demonstrated. the surgical injuries, as such, also reduced the weight increase substantially. still, the loss of t ... | 1989 | 2718734 |
| [viral infection and obstructive cholangiography in children]. | in the last years it has been suggested that idiopathic obstructive cholangiopathy is a pathologic process with evolutive studies: extrahepatic biliary atresia and neonatal hepatitis. the possibility of a viral agent as the inciting factor has been investigated. this report shows four male and one female with confirmed idiopathic obstructive cholangiopathy with different clinical picture. there was evidence of cytomegalovirus infection in two patients, rubella infection in one patient, hepatitis ... | 1989 | 2719422 |
| non-oi vibrio cholerae septicemia associated with a motor vehicle accident. | | 1989 | 2720517 |
| a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds. | focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ... | 1989 | 2721224 |
| a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds. | focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ... | 1989 | 2721224 |
| protection by desferrioxamine against histopathological changes of the liver in the post-oligaemic phase of clinical haemorrhagic shock in dogs: correlation with improved survival rate and recovery. | haemorrhagic shock was produced in anaesthetized dogs, by rapid arterial bleeding to mean arterial blood pressure 35 mmhg, and maintained oligaemic for 4 h followed by return of withdrawn blood(rowb). dogs were observed for 72 h after rowb for survival and recovery, and, for histopathological (hp) studies on liver, dogs were sacrificed 2 h after rowb in non-survival experiments. desferrioxamine mesylate (25 mg/kg) was administered intra-muscularly at 2,3 and 4 h after blood loss in survival expe ... | 1989 | 2722020 |
| vibrio cholerae expresses cell surface antigens during intestinal infection which are not expressed during in vitro culture. | vibrio cholerae o1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env) proteins that were not found on vibrios after in vitro culture in various ordinary liquid media. at the same time, several of the env proteins ordinarily expressed in vitro had disappeared or become much reduced. the infection-induced novel env protein were immunogenic. in immunoblot analyses, antisera rais ... | 1989 | 2722240 |
| bilateral chorioretinitis, centripetal optic neuritis, and encephalitis in a llama. | blindness characterized by dilated, unresponsive pupils and ophthalmoscopic evidence of optic nerve atrophy and retinal degeneration developed in a 5-year-old llama. lesions were not progressive during 2 months of observation. the llama was euthanatized and necropsied. histopathologic findings included chorioretinitis, optic neuritis, and encephalitis characterized by mononuclear perivascular infiltration. attempts to prove an infectious cause were unsuccessful. | 1989 | 2722665 |
| the effects of amphetamine on synaptic plasticity in rat's medial prefrontal cortex. | morphometric analysis of medial prefrontal cortex (layer vi) of rats treated daily with amphetamine in a dose of 2.5 mg/kg during 3 weeks was performed on the electron microscopic level. the efficacy of the amphetamine dosage was tested on behavioral observation. synapses on dendritic shafts and spines were studied. the density of axo-dendritic synapses increase on 74%, while the density of synapses on spine's neck decreased on 53%. most synaptic parameters measured in axo-dendritic (1) and axo- ... | 1989 | 2723411 |
| role of extracellular space in hyperosmotic suppression of potassium-induced electrographic seizures. | 1. focal electrographic seizures arose in the ca1 region of rat hippocampal slices bathed in elevated (8.5 mm) external potassium [( k+]o). high [k+]o also induced spontaneous interictal bursts that originated in area ca3 and propagated to ca1. to examine the contribution to electrographic seizure initiation of excitatory mechanisms that are influenced by extracellular volume, we studied the effect of hyperosmotic expansion of interstitial volume on seizure occurrence, interictal bursts, and exc ... | 1989 | 2723735 |
| synthesis and biological evaluation of s-adenosyl-1,12-diamino-3-thio-9-azadodecane, a multisubstrate adduct inhibitor of spermine synthase. | as part of a continuing search for specific inhibitors of the enzymes involved in polyamine biosynthesis, we have designed and synthesized a multisubstrate adduct inhibitor, s-adenosyl-1,12-diamino-3-thio-9-azadodecane (adodatad), in which critical portions of the nucleophilic aminopropyl acceptor are covalently linked to critical portions of the electrophilic aminopropyl donor to form a potent and specific inhibitor of spermine synthase. in addition, the corresponding desamino analogue which wa ... | 1989 | 2724300 |
| identification of some antigenically related outer-membrane proteins of strains of vibrio cholerae o1 and non-o1 serovars involved in intestinal adhesion and the protective role of antibodies to them. | outer-membrane proteins (omps) of vibrio cholerae strains of o1 and non-o1 serovars were studied. marked similarity was found in the omp profiles of different v. cholerae o1 strains but the omp profile of a non-o1 strain was somewhat different. antigenic relatedness between the omps of different v. cholerae strains was established by enzyme-linked immunosorbent assay (elisa). immunoblotting experiments demonstrated that at least two omps of 36 and 25-26 kda were immunogenic and common to strains ... | 1989 | 2724325 |
| [current status of imported infectious diseases and control policy of the ministry of health and welfare]. | | 1989 | 2724553 |
| [incidence of cholera outbreaks in the world: distribution in the environment and imported seafoods]. | | 1989 | 2724570 |
| alterations in offspring behavior induced by chronic prenatal cocaine dosing. | sperm-positive female long-evans hooded rats were dosed subcutaneously with 10 mg/kg/day cocaine or an equal volume of vehicle (0.9% sterile saline) from gestation day 4 (gd4) through gd18. offspring were assessed for development of negative geotaxis, righting reflex, spontaneous alternation, and open field activity, and for adult behaviors including drl-20 acquisition, water maze, visual discrimination, barbiturate sleep time, shuttlebox avoidance, footshock sensitivity, and tail flick latency. ... | 1989 | 2725438 |
| primary structures of cytochrome p-450 isozyme 5 from rabbit and rat and regulation of species-dependent expression and induction in lung and liver: identification of cytochrome p-450 gene subfamily ivb. | the primary structure of rabbit cytochrome p-450 isozyme 5 has been derived from the nucleotide sequence of cloned cdna. identical sequences were obtained for cdnas constructed with mrna from four different sources, lung and liver of untreated rabbits and liver from rabbits treated once or four times with phenobarbital. isozyme 5 shows significant sequence identity only with rabbit p-450p2 (54%) and rat p-450la omega (53%), which places it in a previously unrecognized cytochrome p-450 gene subfa ... | 1989 | 2725471 |
| tetrahydroaminoacridine and other allosteric antagonists of hippocampal m1 muscarine receptors. | tetrahydroaminoacridine (tha) and a variety of other nonclassical antagonists of muscarine receptors were studied for their ability to bind to primary and allosteric sites on muscarine receptors in rabbit hippocampal membranes. competition curves between 13 antagonists and 1 nm [3h]pirenzepine (kd = 3 nm) were simple mass action curves, but tha produced steeper curves, indicating positive cooperativity. nonetheless, tha inhibited the binding of low concentrations of [3h]pirenzepine, [3h]n-methyl ... | 1989 | 2725474 |
| electrophoretic analysis of histoplasma capsulatum chromosomal dna. | seven chromosome-sized dna molecules in the downs strain of histoplasma capsulatum were resolved by using chromosome-specific dna probes in blot hybridizations of contour-clamped homogeneous electric field (chef) and field-inversion gel electrophoresis (fige) agarose gels. the sizes of the chromosomal dna bands extended from that of the largest saccharomyces cerevisiae chromosome to beyond that of the schizosaccharomyces pombe chromosomes. under our experimental conditions, the order of the five ... | 1989 | 2725508 |
| characterization and purification of h1tf2, a novel ccaat-binding protein that interacts with a histone h1 subtype-specific consensus element. | definition of mechanisms regulating human histone h1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. two distinct binding activities have been identified in nuclear extracts from hela cells and mapped within a 180-base-pair (bp) region of a cell cycle-regulated h1 gene promoter. h1tf1 bound to an h1-specific a + c-rich sequence (ac box), 100 bp upstream of the cap site; h1tf2 inter ... | 1989 | 2725515 |
| primary structures of sm31/32 diagnostic proteins of schistosoma mansoni and their identification as proteases. | we have constructed cdna clones containing the complete nucleotide sequences coding for two highly antigenic schistosoma mansoni adult worm proteins, sm31 and sm32. the predicted amino acid sequence of sm31 shows significant homology to mouse, rat and human cathepsin b. the nucleotide sequence of sm32 is identical to that reported by others for s. mansoni "haemoglobinase'. the different nucleotide sequences demonstrate the existence of two different proteolytic enzymes, both of which are synthes ... | 1989 | 2725581 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 24-1989. a 77-year-old man with back pain and acute inability to walk. | | 1989 | 2725601 |
| effects of reserpine on dopamine metabolite in the nucleus accumbens and locomotor activity in freely moving rats. | the effect of reserpine (2 mg/kg i.p.) on both locomotor activity and the turnover of dopamine metabolite in the rat nucleus accumbens was estimated by using an activity monitor (animex) and by in vivo brain microdialysis. three to five hours after reserpine administration locomotor activity was reduced and there was a concomitant increase in the level of the dopamine metabolite, homovanillic these findings suggest that depletion of dopamine from the nucleus accumbens may result in decreased loc ... | 1989 | 2725828 |
| electrical and mechanical properties of spontaneous contraction in hypertensive rat portal vein. | contractile and electrical activities of longitudinal smooth muscle of portal vein from normotensive wistar kyoto rats (wky) and stroke-prone spontaneously hypertensive rats (shrsp) were compared. amplitude and duration of spontaneous contraction of shrsp portal vein were greater than those of wky portal vein. no significant differences were observed in the resting membrane potentials between these preparations. spontaneous spike activity appeared as a form of bursts. duration of the burst and t ... | 1989 | 2726435 |
| conjugated diene production after airway acid aspiration. | | 1989 | 2726984 |
| [use of the dna-dna hybridization method on filters for determining the toxic potency of atypical strains of vibrio cholerae isolated from natural sources]. | the methods of the radioimmunoassay and the blot hybridization of restricted fragments of chromosomal dna have been used for the characterization of v. cholerae atypical strains isolated from the natural environment. for all strains under study, the radioimmunoassay has been found to yield the most sharply defined data characterizing their atoxigenicity. the absence of the structural genes of toxin in the chromosomes of these strains has been shown by the method of blot hybridization. some metho ... | 1989 | 2728700 |
| distribution of different fiber types in whole cross-sections of human platysma. a histological and morphometric study. | analysis of cryostat cross-sections of the entire platysma muscle from human autopsies revealed enzyme histochemical and morphometric differences between normal human limb and facial muscles. the mean diameter of platysma fibers was about 50% of that of normal limb muscle fibers. fiber type diameter increased from the medial to the lateral parts of the platysma. a variability coefficient of 356 indicated great variation in fiber caliber, with many fibers of 10 microns and less. type i fibers sho ... | 1989 | 2728841 |
| negative ion fast atom bombardment mass spectrometry for native gangliosides using a neutral matrix. | a hexamethylphosphoric triamide proved to be a useful solvent for negative ion fast atom bombardment mass spectrometry (fabms) of underivatized gangliosides, using a conventional glycerol matrix. analysis of the gangliosides using a hexamethylphosphoric triamide was more convenient and more efficient not only for molecular weight determination but also for elucidating the structure of the carbohydrate sequence. we also noted the significance of the high polarity of the solvent as well as the ele ... | 1989 | 2729549 |
| negative ion fast atom bombardment mass spectrometry for native gangliosides using a neutral matrix. | a hexamethylphosphoric triamide proved to be a useful solvent for negative ion fast atom bombardment mass spectrometry (fabms) of underivatized gangliosides, using a conventional glycerol matrix. analysis of the gangliosides using a hexamethylphosphoric triamide was more convenient and more efficient not only for molecular weight determination but also for elucidating the structure of the carbohydrate sequence. we also noted the significance of the high polarity of the solvent as well as the ele ... | 1989 | 2729549 |
| isoelectric focusing under dissociating conditions for analysis of muscle protein from clinically normal dogs and labrador retrievers with hereditary myopathy. | protein profiles of whole homogenates of anconeus (slow twitch) and biceps femoris (fast twitch) muscles of clinically normal dogs and of labrador retrievers with hereditary myopathy (hm) were resolved on flat bed polyacrylamide isoelectric-focusing gels. three methods of sample solubilization were performed. the solubilization buffer, with high concentrations of urea, precipitated the zwitterionic detergent, but use of the buffer containing 3% np-40, 9.2m urea, and 0.1m arginine resulted in bet ... | 1989 | 2729710 |
| large phosphate shifts with treatment for hyperglycemia. | although hypophosphatemia is known to commonly accompany therapy for hyperglycemia, it is not generally appreciated that severe life-threatening depletion of phosphate may occur. i followed up two patients who had precipitous drops during intravenous insulin therapy for diabetic ketoacidosis and hyperosmolar nonketotic state. the patient with diabetic ketoacidosis had a phosphate level nadir that equaled the lowest recorded phosphate level in a living human (0.03 mmol/l). because (1) serum phosp ... | 1989 | 2730261 |
| in vivo dna cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse l1210 leukemia and normal bone marrow cells. | alkaline elution was done on a variety of cells following cyclophosphamide (cy) treatment in vivo. cells used were l1210 leukemia, normal mouse bone marrow, and peripheral blood cells obtained from a patient with chronic lymphatic leukemia (cll). endpoints used were determination of single strand breaks, dna-dna interstrand and dna-protein cross-links. after treatment of mice with cy (4 mg/mouse), low levels of single strand breaks were observed in both l1210 leukemia and cdf1 normal bone marrow ... | 1989 | 2731167 |
| age-related induction and disappearance of carcinogen-dna-adducts in livers of rats exposed to low levels of 2-acetylaminofluorene. | it was investigated whether in vivo aging of rat liver is associated with changes in the induction and rate of disappearance of dna damage. for this purpose 6- and 36-month-old rats were intraperitoneally injected with a single, low dose (5 mg/kg body wt.) of the model liver carcinogen 2-acetylaminofluorene (aaf). using the 32p-postlabeling assay we found that n-(deoxyguanosin-8-yl)-2-aminofluorene (dg-c8-af) was the major dna-adduct formed. the minor adduct n-(deoxyguanosin-8-yl)-2-acetylaminof ... | 1989 | 2731307 |