| [sensitivity to cefoperazone (cefobid) of microorganisms isolated from diagnostic specimens]. | sensitivity of cephaperasone (cefobid) of 966 straits of microorganisms, isolated from 661 patients was assessed. the study was carried out according to the trial described by bauer et al, using the mueller-hinton medium and original cephaperasone tests (pfizer). full sensitivity was found in 87.1% of the organisms. the following conclusions were made: 1. most isolated organisms were sensitivite to cephaperasone (87.1%); e. coli (98.5%), kl. pneumoniae (94.8%), atrobacter (93.1%), enterobacter ( ... | 1989 | 2517638 |
| the microbial ecology of soybean soaking for tempe production. | soybeans soaked in tap water for 24 to 36 h at 20, 30 or 37 degrees c underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/ml (depending on temperature) and a reduction of ph from 6.5 to 4.5. lactobacillus casei, streptococcus faecium, staphylococcus epidermidis and streptococcus dysgalactiae dominated the fermentation but, significant contributions were also made by klebsiella pneumoniae, klebsiella ozaenae, enterobacter cloacae, enterobact ... | 1989 | 2518226 |
| [urinary tract infections in primary care: predominant microbes and their antibiotic sensitivity]. | we have prospectively evaluated 227 episodes of community-acquired urinary tract infection (uti) from october 1st to december, 15th, 1987. the urine samples, adequately collected, were processed in the bacteriology laboratory of the albacete general hospital. e. coli was the most commonly isolated organism (62%), followed by proteus mirabilis (6.6%), streptococcus faecalis (6.6%), serratia marcescens (6.6%) and klebsiella pneumoniae (6.2%). as regards the sensitivity of e. coli to the most commo ... | 1989 | 2518913 |
| "orostim"--polymicrobial preparation for oral administration. i. "in vivo" determination of toxicity and of inborn resistance stimulation characteristic. | "orostim" is a polymicrobial immunomodulator for oral administration, obtained from bacterial suspensions, disintegrated by ultrasonics and dried by atomization. the preparation was chemically characterized before and after atomization without presenting essential modifications. orostim was not shown to be toxic in mice and rats by esophageal intubation, as long as 20 days. the animals presented normal evolution; hemoleukograms, serum proteins and alkaline phosphatase, in rats, did not present s ... | 1989 | 2520673 |
| identification, cloning, and sequence analysis of the nitrogen regulation gene ntrc of agrobacterium tumefaciens c58. | we describe the cloning of an ntrc gene of agrobacterium tumefaciens c58 by interspecific complementation of an escherichia coli ntrc mutant. restriction mapping and southern blot analysis of the complementing clone identified a 1.7-kb ecori-pvuii dna fragment whose sequence was determined. analysis of this sequence revealed coding regions corresponding to a complete ntrc gene and the c-terminal region of an ntrb gene. amino acid sequence comparisons of a. tumefaciens ntrc protein with ntrc sequ ... | 1989 | 2520824 |
| [antibacterial effect of cefixime]. | cefixime (cfm) is a new hemi-synthetic orally active cephalosporin which exhibits a particular affinity for pbps 3, 1a, 1bs. its penetration through the gram negative bacilli outer membrane is similar to that of third generation cephalosporins. the mics were assessed by the agar dilution method against 2,489 bacterial strains collected in 10 hospitals. against enterobacteriaceae, mics50 and 90 are respectively (mg/l): naturally non beta-lactamase-producing species: e. coli and shigella: 0.25-0.5 ... | 1989 | 2530528 |
| [antibacterial effect of cefixime in the presence of the type of beta-lactamases produced by enterobacteriaceae]. | the susceptibility of isolates or enterobacteriaceae to orally absorbed beta-lactams (amoxicillin, cephalexin, cefaclor, cefuroxime, and cefixime), was maximum for the iminomethoxy-aminothiazolyl-cephalosporin but variable according to bacterial species. for e. coli, p. mirabilis, salmonella, shigella, k. pneumoniae, k. oxytoca (group 1) mic50 were congruent to 0.06 mg/l, and mic90 congruent to 0.12 mg/l. finally for c. freundii, e. aerogenes, e. cloacae, s. marcescens, m. morganii, mic50 were h ... | 1989 | 2530532 |
| [in vitro comparative bactericidal effect of cefotaxime and cefixime in a kinetic model]. | the bactericidal activity of cefotaxime against escherichia coli, klebsiella pneumoniae and serratia marcescens, was compared with that of cefixime in an in vitro model simulating the human pharmacokinetics of these antibiotics. kinetic parameters in this model were t1/2 = 1.3 h and cmax = 45 mg/l for cefotaxime; t1/2 = 3.5 h and cmax = 5 or 3.5 mg/l for cefixime. these parameters mimicked those obtained after a 1 g intravenous infusion of cefotaxime and an oral uptake of 0.4 or 0.2 g of cefixim ... | 1989 | 2530534 |
| novel aerobactin receptor in klebsiella pneumoniae. | several klebsiella pneumoniae strains which produced enterochelin but not aerobactin were nevertheless sensitive to cloacin df13. in contrast, a strain of serotype k1:o1 which produced both siderophores was cloacin-resistant. loss by mutation of the o1 but not k1 antigen rendered this strain cloacin-sensitive, indicating that the o1 antigen prevented access of cloacin to the cloacin/aerobactin receptor. unlike the k1:o1 strain, the aerobactin-negative strains failed to hybridize in a colony blot ... | 1989 | 2534599 |
| potentiation by nonspecific immunostimulation of the efficacy of antibiotics in the treatment of experimental bacterial infections. | in the present investigation, mouse infection models with either intracellular or extracellular bacteria were designed in order to assess the effect of nonspecific immunostimulation with the c. granulosum-derived p40 immunomodulator on infection treatment, performed with doses of antibiotics achieving a low percentage of cures. the results obtained showed that nonspecific immunostimulation was able to significantly enhance antibiotic therapy efficacy. in addition, combined treatments of bacteria ... | 1989 | 2534887 |
| the azurin gene from pseudomonas aeruginosa. cloning and characterization. | we have cloned and sequenced the pseudomonas aeruginosa azurin structural gene and its flanking regions. the dna sequence predicts a pre-protein with a signal peptide of 19 amino acids followed by the 128-amino-acid mature azurin protein. nuclease-s1 mapping and primer elongation experiments indicated two 5' termini of the azurin transcript. the major transcript of the azurin gene is initiated around 35 base pairs upstream from the translational start. the minor transcript, with a promoter regio ... | 1989 | 2537198 |
| comparison of tellurite resistance determinants from the incp alpha plasmid rp4ter and the inchii plasmid phh1508a. | the tellurite resistance (ter) determinants of the inchii plasmid phh1508a and the broad host range incp alpha plasmid rp4ter were cloned into puc8, creating plasmids pdt1364 and pdt1558, respectively. the ter region of pdt1364 was localized to a 1.25-kilobase region by using tn1000 insertion mutagenesis. insertions of tn1000 into pdt1558 which resulted in tellurite sensitivity spanned 1.75 kilobases of dna. no similarity between the restriction maps of these two plasmids was observed, and no ho ... | 1989 | 2539362 |
| the separate roles of pqq and apo-enzyme syntheses in the regulation of glucose dehydrogenase activity in klebsiella pneumoniae nctc 418. | no holoenzyme pyrroloquinoline quinone (pqq)-dependent glucose dehydrogenase and only very low apoenzyme levels could be detected in cells of klebsiella pneumoniae, growing anaerobically, or carrying out a fumarate or nitrate respiration. low glucose dehydrogenase activity in some aerobic glucose-excess cultures of k. pneumoniae (ammonia or sulphate limitation) was increased significantly by addition of pqq, whereas in cells already possessing a high glucose dehydrogenase activity (phosphate or ... | 1989 | 2539792 |
| genetic regulation of nitrate assimilation in klebsiella pneumoniae m5al. | we isolated mu di1734 insertion mutants of klebsiella pneumoniae that were unable to assimilate nitrate or nitrite as the sole nitrogen source during aerobic growth (nas- phenotype). the mutants were not altered in respiratory (anaerobic) nitrate and nitrite reduction or in general nitrogen control. the mutations were linked and thus defined a single locus (nas) containing genes required for nitrate assimilation. beta-galactosidase synthesis in nas+/phi(nas-lacz) merodiploid strains was induced ... | 1989 | 2540153 |
| the use of lambda plac-mu hybrid phages in klebsiella pneumoniae and the isolation of stable hfr strains. | klebsiella pneumoniae 1033-5p14 and its p1-sensitive derivative kay2026 were found to be resistant to lambda although they contained a lamb protein, active as a maltoporin. sensitive derivatives could only be obtained after introduction of the ptroy9 plasmid which expresses lamb and the corresponding lambda receptor from escherichia coli k12 at high levels. lysogenic derivatives from such strains were shown to carry the phage at secondary att sites and to give high titer lysates when induced. th ... | 1989 | 2540416 |
| substitution of histidine for arginine-101 of dinitrogenase reductase disrupts electron transfer to dinitrogenase. | dinitrogenase reductase from klebsiella pneumoniae strain un1041 has a histidine residue substituted for arginine at position 101. the mutant dinitrogenase reductase was purified and characterized in order to determine the importance of arginine-101 in the interaction between dinitrogenase and dinitrogenase reductase during electron transfer. purified dinitrogenase reductase from un1041 is a dimer of 67 kda, contains a functional 4fe-4s cluster, undergoes a mgatp-dependent conformational change, ... | 1989 | 2540818 |
| mutations in the rna polymerase recognition sequence of the klebsiella pneumoniae nifh promoter permitting transcriptional activation in the absence of nifa binding to upstream activator sequences. | positive control of the wild-type klebsiella pneumoniae nifh promoter by the nifa protein requires that nifa is bound at the upstream activator sequence (uas). by introducing base substitutions at -15 to -17 in the rna polymerase recognition sequence of the nifh promoter, positive control by a form of nifa unable to bind to the uas was greatly increased when compared to the wild-type promoter. transcriptional activation still required the rpon encoded sigma factor and was initiated at the same n ... | 1989 | 2541410 |
| [microbiological study results (bacteriology and electron microscopy) of diarrhea in dog whelps]. | bacteriological examinations of 159 faeces and intestinal contents of dogs with diarrhoea revealed e. coli in 157 specimens. 73 of these samples contained non haemolytic strains, 18 haemolytic isolates, and 66 haemolytic, as well as non haemolytic strains. klebsiella sp. and staphylococcus aureus were found in 9 cases, and salmonella sp. (group b) was isolated once only. by electron microscopy parvovirus could be detected in 19 samples. ten were positive for coronavirus and one for rotavirus. mo ... | 1989 | 2541519 |
| molecular cloning and heterologous expression of a klebsiella pneumoniae gene encoding alginate lyase. | the alginate lyase (aly; guluronate specific)-coding gene of klebsiella pneumoniae was cloned using the cosmid vector pmmb33, transduced into escherichia coli and expressed in this host. four aly-positive clones with unstable phenotypes were identified out of 700 kanamycin-resistant transductants. a stable derivative of one of the clones was studied further and contained 12.1-kb of insert dna. the aly-coding gene (aly), still partially under the control of its native promoter, was localised with ... | 1989 | 2542130 |
| antimicrobial activity of clove oil dispersed in a concentrated sugar solution. | essential oil of clove, dispersed (0.4% v/v) in a concentrated sugar solution, had a marked germicidal effect against various bacteria and candida albicans. staphylococcus aureus (five strains), klebsiella pneumoniae, pseudomonas aeruginosa, clostridium perfringens, and escherichia coli inoculated at a level of 10(7) cfu/ml, and c. albicans (inoculum 4.0 x 10(5) cfu/ml) were killed (greater than 99.999%) after 2-7 min in a laboratory broth supplemented with 63% (v/w) of sugar, and containing 0.4 ... | 1989 | 2542213 |
| rapid detection of bordetella pertussis by a monoclonal antibody-based colony blot assay. | monoclonal antibodies to bordetella pertussis filamentous hemagglutinin (fha) and lipopolysaccharide (lps) were used in a colony blot enzyme-linked immunosorbent assay designed for rapid detection of b. pertussis. bacterial colonies from bordet-gengou agar plates were blotted onto nitrocellulose filter disks, lysed by immersion in chloroform, and reacted with monoclonal antibodies. following reaction with peroxidase-conjugated rabbit anti-mouse immunoglobulin antisera and 4-chloro-1-naphthol, bl ... | 1989 | 2542357 |
| conversion of 5-s-ethyl-5-thio-d-ribose to ethionine in klebsiella pneumoniae. basis for the selective toxicity of 5-s-ethyl-5-thio-d-ribose. | 5-s-ethyl-5-thio-d-ribose (ethylthioribose) exhibits antiprotozoal activity against plasmodium falciparum, giardia lamblia, and ochromonas malhamensis, but is nontoxic to cultured human and murine bone marrow cells (riscoe, m. k., ferro, a. j., and fitchen, j. h. (1988) antimicrob. agents chemother. 32, 1904-1906). we propose the following mechanism to account for the observed selective toxicity of ethylthioribose. 1) the cytocidal action of ethylthioribose against protozoa is a result of its co ... | 1989 | 2543672 |
| outreading promoters are located at both ends of the gamma-delta transposon. | two plasmids were isolated containing oppositely oriented gamma-delta insertions between the wild-type transcription initiation site of the nifhdky operon and the nifh coding sequence. the nifhdky promoter of klebsiella pneumoniae, similar to other nitrogen fixation (nif) promoters, normally requires the products of ntra and nifa for activity. mutations that allowed constitutive expression of the nifhdky operon were searched for by transforming a plasmid, containing the regulatory region of this ... | 1989 | 2543904 |
| a primary respiratory na+ pump of an anaerobic bacterium: the na+-dependent nadh:quinone oxidoreductase of klebsiella pneumoniae. | membranes of klebsiella pneumoniae, grown anaerobically on citrate, contain a nadh oxidase activity that is activated specifically by na+ or li+ ions and effectively inhibited by 2-heptyl-4-hydroxyquinoline-n-oxide (hqno). cytochromes b and d were present in the membranes, and the steady state reduction level of cytochrome b increased on nacl addition. inverted bacterial membrane vesicles accumulated na+ ions upon nadh oxidation. na+ uptake was completely inhibited by monensin and by hqno and sl ... | 1989 | 2545175 |
| human placental 17 beta-hydroxysteroid dehydrogenase is homologous to nodg protein of rhizobium meliloti. | the amino acid sequence of human placental 17 beta-hydroxysteroid dehydrogenase (17 beta-oh-steroid dehydrogenase) was found to be similar to that of the nodg protein of rhizobium meliloti. the computer-based comparison score is 11.5 sd higher than that obtained with 2500 comparisons of randomized sequences of these proteins. the probability of getting such a score by chance is 6 x 10(-31). 17 beta-oh-steroid dehydrogenase is also similar to klebsiella aerogenes ribitol dehydrogenase and escheri ... | 1989 | 2547159 |
| role of leukotriene b4 in the pathogenesis of klebsiella pneumoniae-induced bovine mastitis. | mastitis was induced in 4 lactating cows by inoculation of klebsiella pneumoniae (10(7) organisms/ml) via the teat canal. sterile isotonic saline solution (1 ml) was instilled into designated control quarters via the teat canal. changes in milk leukotriene b4 and c4 (ltb4, ltc4) concentrations, milk somatic cell counts, and milk bovine serum albumin concentration were monitored over a 24-hour postinoculation period. milk ltb4 concentration before inoculation in control quarters and quarters late ... | 1989 | 2548422 |
| the sodium ion translocating oxaloacetate decarboxylase of klebsiella pneumoniae. sequence of the integral membrane-bound subunits beta and gamma. | the sequences upstream and downstream of the cloned gene for the alpha-subunit of the na+ pump oxaloacetate decarboxylase of klebsiella pneumonia were determined. an open reading frame in the upstream region was identified as the gene for the gamma-subunit, and an open reading frame in the downstream region represents the gene for the beta-subunit. the deduced primary structure of the gamma- and beta-subunit was confirmed by protein sequencing of about 37 and 22%, respectively, of each polypepti ... | 1989 | 2549031 |
| proof of the occurrence of 5,6-o-(1-carboxyethylidene)-d-galactofuranose units in the capsular polysaccharide of klebsiella k12. | the 13c-n.m.r. spectra of the capsular polysaccharide of klebsiella k41 and phage-derived oligosaccharides k41-p1 and k41-p2 were compared with spectra from the structurally similar polysaccharide of klebsiella k12 and oligosaccharides k12-p1 and k12-p2. this led to the conclusion that k41 and k12 contain one and two galactofuranose residues per repeating unit, respectively, and that the terminal, lateral residue in k12 has the 5,6-o-(1-carboxyethylidene)-d-galactofuranose structure rather than ... | 1989 | 2550127 |
| in vitro activity of ytr 830. | ytr 830, now known as tazobactam, is a new penicillanic acid sulfone beta-lactamase inhibitor. the in vitro activity of ytr 830 combined with various penicillins was determined and compared to that of clavulanate and sulbactam combined with the same agents. combined with ampicillin or amoxicillin, all three inhibitors were active against beta-lactamase producing strains of staphylococcus aureus, haemophilus influenzae, klebsiella, citrobacter diversus, and all anaerobes except for bacteroides fr ... | 1989 | 2552029 |
| in vitro and in vivo efficacy of ytr-830h and piperacillin combinations versus beta-lactamase-producing bacteria. | ytr-830h, a beta-lactamase inhibitor, is a non-amino penicillanic sulfone. in vitro synergistic activity with piperacillin was determined for 226 beta-lactamase producing clinical cultures. combination of piperacillin: ytr in ratios of 2:1, 4:1, and 8:1 were highly effective vs escherichia coli, proteus, providencia, morganella, staphylococcus, and bacteroides. minimum inhibitory concentrations (mics) of piperacillin were reduced from the resistant to susceptible range. the higher ratios were le ... | 1989 | 2552030 |
| the nifa gene product from rhizobium leguminosarum biovar trifolii lacks the n-terminal domain found in other nifa proteins. | the nifa gene has been identified between the fixx and nifb genes in the clover microsymbiont rhizobium leguminosarum biovar trifolii (r.i. bv. trifolii) strain anu843. expression of the nifa gene is induced in the symbiotic state and site-directed mutagenesis experiments indicate that nifa expression is essential for symbiotic nitrogen fixation. interestingly, the predicted r.i. bv. trifolii nifa protein lacks an n-terminal domain that is present in the homologous proteins from r.i. bv. viciae, ... | 1989 | 2552256 |
| comparative in vitro activities of piperacillin-tazobactam and ticarcillin-clavulanate. | the in vitro activities of ticarcillin, piperacillin, clavulanic acid, tazobactam, ticarcillin-clavulanate, and piperacillin-tazobactam against 819 bacterial isolates were compared. the two beta-lactamase inhibitors, clavulanic acid and tazobactam, had little useful antibacterial activity but enhanced the activities of the penicillins against beta-lactamase-producing strains of haemophilus influenzae, branhamella catarrhalis, and methicillin-susceptible staphylococcus aureus; all strains were su ... | 1989 | 2552904 |
| [usefulness of endotoxin removal from the septic blood with direct hemoperfusion using pmx-f]. | sepsis and septic shock have been drugs difficult to treat despite many our therapeutic methods. there are many drugs for sepsis, but few can remove or detoxify such sabstances as endotoxin. the polystyrene fiber immobilized polymyxin b (pmx-f) have been invented and it was already confirmed the stability of its bonding. in this paper, mass-productive pmx-f for the clinical application were estimated. in vitro studies: pmx-f adsorbed endotoxin (10ng/ml) from the calf serum, and recombinant tumor ... | 1989 | 2555663 |
| isolation and characterization of oxaloacetate decarboxylase of salmonella typhimurium, a sodium ion pump. | anaerobic growth of salmonella typhimurium on citrate is na+-dependent and requires induction of the necessary enzymes during a 20-40 h lag phase. the citrate fermentation pathway involves citrate lyase and oxaloacetate decarboxylase. the decarboxylase is a membrane-bound, na+-activated, biotin-containing enzyme that functions as a na+ pump. oxaloacetate decarboxylase was isolated by affinity chromatography of a triton x-100 extract of the bacterial membranes on avidin-sepharose. the enzyme cons ... | 1989 | 2556085 |
| phosphate regulon in members of the family enterobacteriaceae: comparison of the phob-phor operons of escherichia coli, shigella dysenteriae, and klebsiella pneumoniae. | the structure and function of the phob and phor genes of shigella dysenteriae strains and klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. complementation tests among the genes of escherichia coli, s. dysenteriae strains, and k. pneumoniae for production of alkaline phosphatase indicate that s. dysenteriae serotype 2 and serotype 3 strains and k. pneumoniae are phoa+ phob+ phor+ but s. dysenteriae sh and serotype 1 strains are phoa phob+ phor. nucl ... | 1989 | 2556368 |
| synergistic interaction of josamycin with human neutrophils bactericidal function in vitro. | josamycin, a 16-membered ring macrolide is concentrated up to 20-fold in phagocytic cells compared with serum. we have studied the in-vitro interaction of this drug with human neutrophils (pmn) bactericidal function by using two strains resistant to this antibiotic, pseudomonas aeruginosa and klebsiella pneumoniae, and a sensitive one, staphylococcus aureus 209p. it was shown that josamycin-pretreated adherent pmn displayed an increased phagocytic activity (about 30 to 40%) for s. aureus or k. p ... | 1989 | 2557317 |
| comparative effects of f1 and p1 fractions obtained from a klebsiella pneumoniae glycoproteic extract (ru 41740) on polymorphonuclear leukocytes. | ru 41740 is a glycoprotein extract from klebsiella pneumoniae described as a macromolecular aggregation of a lipopolysaccharide (lps)-associated protein (f1 fraction) and a glycoproteic complex (p1 fraction). the human polymorphonuclear (pmn) response was studied after incubation of the cells in the presence of ru 41740, f1 and p1 fractions, or f1-p1 complex. oxidative metabolism was assessed by chemiluminescence, o2 consumption, o2- generation, and degranulation by beta-glucuronidase release. r ... | 1989 | 2557390 |
| site-directed mutagenesis of the klebsiella pneumoniae nitrogenase. effects of modifying conserved cysteine residues in the alpha- and beta-subunits. | the five conserved cysteine residues present in the alpha-subunit and the three conserved cysteine residues present in the beta-subunit of nitrogenase component 1 were individually changed to alanine. mutations in the alpha-subunit at positions 63, 89, 155 and 275 and in the beta-subunit at positions 69, 94 and 152 all resulted in a loss of diazotrophic growth and component 1 activity and loss of the normal e.p.r. signal of the component 1 protein. component 2 activity was retained. replacement ... | 1989 | 2557830 |
| multiplicity of tem-derived beta-lactamases from klebsiella pneumoniae strains isolated at the same hospital and relationships between the responsible plasmids. | five plasmid-mediated beta-lactamases conferring high-level resistance to ceftazidime were isolated from klebsiella pneumoniae strains in the same hospital. these enzymes had isoelectric points ranging from 5.3 to 6.5 (caz-1, 5.55; caz-2, 6.0; caz-3, 5.3; caz-6, 6.5; and caz-7, 6.3). all isolates and their escherichia coli transconjugants were highly resistant to amoxicillin (mics, greater than 4,096 micrograms/ml), piperacillin (64 to 256 micrograms/ml), cephalothin (32 to 256 micrograms/ml), a ... | 1989 | 2558614 |
| comparative in vitro and in vivo activities of piperacillin combined with the beta-lactamase inhibitors tazobactam, clavulanic acid, and sulbactam. | tazobactam (ytr-830h), a novel beta-lactamase inhibitor, was compared with clavulanic acid and sulbactam for enhancement of the activity of piperacillin against beta-lactamase-producing, piperacillin-resistant clinical isolates. piperacillin mics were determined in media containing a fixed concentration of 2 or 4 micrograms of the inhibitors per ml. the higher concentration was generally more effective. tazobactam was superior to sulbactam in enhancing the spectrum and potency of piperacillin. a ... | 1989 | 2558615 |
| in vitro activity of at-4140 against clinical bacterial isolates. | the activity of at-4140, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with those of existing analogs. at-4140 had a broad spectrum and a potent activity against gram-positive and -negative bacteria, including legionella spp. and bacteroides fragilis. the activity of at-4140 against gram-positive and -negative cocci, including acinetobacter calcoaceticus, was higher than those of ciprofloxacin, ofloxacin, and norfloxacin. its activity again ... | 1989 | 2558617 |
| comparison of the in-vitro effect of several macrolides on the oxidative burst of human neutrophils. | we have compared the in-vitro interaction of five macrolides (roxithromycin, erythromycin, spiramycin, oleandomycin and josamycin) with human neutrophils (pmn). only roxithromycin strongly impaired the oxidative burst of pmn assessed by luminol amplified chemiluminescence, superoxide anion generation, and myeloperoxidase-mediated iodination of proteins. this effect was observed only for high concentrations of this drug (100 and 50 mg/l). furthermore, the sensitivity of pmn to the depressive effe ... | 1989 | 2559072 |
| identification of a regulatory nifa type gene and physical mapping of cloned new nif regions of azospirillum brasilense. | three new tn5-mutagenized nif genes of azospirillum brasilense were characterized. the sizes of the restriction fragments and the restriction maps of the cloned nif dna regions showed that these nif genes are distinct from those reported earlier, e.g. nifhdk, nife, nifus, fixabc. the nif27 mutant was identified as a nifa type regulatory gene of a. brasilense (a) by genetic complementation with nifa of klebsiella pneumoniae, (b) by the absence of nitrogenase iron protein in western protein blots ... | 1989 | 2559312 |
| anaerobic growth of escherichia coli on glycerol by importing genes of the dha regulon from klebsiella pneumoniae. | the dha regulon of klebsiella pneumoniae specifying fermentative dissimilation of glycerol was mobilized by the broad-host-range plasmid rp4:mini mu and introduced conjugatively into escherichia coli. the recipient e. coli was enabled to grow anaerobically on glycerol without added hydrogen acceptors, although its cell yield was less than that of k. pneumoniae. the reduced cell yield was probably due to the lack of the coenzyme-b12-dependent glycerol dehydratase of the dha system. this enzyme in ... | 1989 | 2559947 |
| tazobactam prevention of emergence of resistance. | | 1989 | 2560419 |
| effects of the yeast extract components pyrroloquinoline quinone and aspartic acid on vitamin b12 production in klebsiella pneumoniae ifo 13541. | the production of vitamin b12 from carbohydrates, peptone, casamino acid, etc., by intestinal bacteria was investigated. klebsiella pneumoniae ifo 13541 was the most efficient strain for vitamin b12 production, which depended exclusively on the concentration of yeast extract added to the medium. a concentrated solution of yeast extract (1 ml) was chromatographed on a sephadex g-25 column (1 x 180 cm) and eluted with h2o (eighty fractions of 3 ml each were collected). it was found that fractions ... | 1989 | 2561367 |
| type 3 fimbriae among enterobacteria and the ability of spermidine to inhibit mr/k hemagglutination. | the distribution of the gene cluster encoding type 3 fimbriae among various isolates of the family enterobacteriaceae was investigated by using 112 clinical and nonclinical isolates. closely related dna sequences were detected in all klebsiella strains, in most enterobacter isolates, in a smaller number of escherichia coli and salmonella spp., and in a single isolate each of yersinia enterocolitica and serratia liquefaciens but not in isolates of morganella or providencia species or serratia mar ... | 1989 | 2562835 |
| expression of type 1 fimbriae and mannose-sensitive hemagglutinin by recombinant plasmids. | deletions within the cloned genes (fima) encoding the type 1 major fimbrial subunits of two isolates of klebsiella pneumoniae resulted in a nonfimbriate but hemagglutinating phenotype after transformation of escherichia coli hb101 or orn103. phenotypic expression of type 1 fimbriae could be restored by transformation with plasmids containing the fima genes of the fimbrial gene clusters from different strains. the surface fimbriae expressed were serologically identical to those of the polymerized ... | 1989 | 2563717 |
| identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of klebsiella pneumoniae. | strains of klebsiella pneumoniae are known to express two morphologically and functionally distinct filaments, the type 3 and the type 1 fimbriae. the gene (mrkd) encoding the adhesion of k. pneumoniae type 3 fimbriae was identified by transcomplementation analysis with the pap fimbrial gene cluster of escherichia coli. the nucleotide sequence of the mrkd gene was determined. in addition, the determinant coding for the k. pneumoniae type 1 fimbrial adhesion was identified, and its nucleotide seq ... | 1989 | 2563996 |
| rhizobium meliloti 1021 has three differentially regulated loci involved in glutamine biosynthesis, none of which is essential for symbiotic nitrogen fixation. | we have cloned and characterized three distinct rhizobium meliloti loci involved in glutamine biosynthesis (glna, glnii, and glnt). the glna locus shares dna homology with the glna gene of klebsiella pneumoniae, encodes a 55,000-dalton monomer subunit of the heat-stable glutamine synthetase (gs) protein (gsi), and complemented an escherichia coli glna mutation. the glnii locus shares dna homology with the glnii gene of bradyrhizobium japonicum and encodes a 36,000-dalton monomer subunit of the h ... | 1989 | 2563998 |
| klebsiella pneumoniae infection complicating a puncture wound of the foot: a case report. | klebsiella pneumoniae infections of the feet are rare following puncture wounds. we present a case of such an infection following a nail injury, and stress that there is nothing distinctive about the clinical presentation with this organism and that bacterial cultures and sensitivity tests of isolates are necessary for proper wound management. | 1989 | 2564175 |
| quantitative bacterial cultures and beta-lactamase activity in chronic suppurative otitis media. | aspiration of the exudate through open perforation was performed in 54 children with chronic suppurative otitis media. eighty aerobic and 81 anaerobic isolates were recovered. aerobic bacteria only were involved in 20 patients (37%), and anaerobic organisms only in seven (13%). mixed aerobic and anaerobic isolates were recovered from 27 patients (50%). the most common bacteria isolated were anaerobic gram-positive cocci, bacteroides melaninogenicus group, pseudomonas aeruginosa, klebsiella pneum ... | 1989 | 2565099 |
| effect of biostim (ru 41.740) on natural killer cell generation from bone marrow precursors. | we have evaluated the possible effect of ru 41.740 (biostim), a mixture of two glycoproteins extracted from k. pneumoniae, on the in vitro interleukin-2 (il-2)-induced generation of nk cells from bone marrow (bm) precursors and on the in vivo reconstitution of splenic nk activity in lethally irradiated (9 gy) and bm reconstituted mice. our results show that ru 41.740 is able to augment the generation of nk cells when added (1-0.01 micrograms/ml) to normal or 5-fluorouracil-resistant bm, cultured ... | 1989 | 2565297 |
| ecological impact of ampicillin and cefuroxime in neonatal units. | the relation between local antibiotic consumption and faecal carriage of beta-lactam-resistant strains of escherichia coli and klebsiella spp was studied in 953 children discharged from twenty-two neonatal units. an increased rate of carriers of klebsiella spp strains resistant to ampicillin, cefuroxime, and cephalexin and of e coli strains resistant to ampicillin was associated with the use of ampicillin, with or without gentamicin. ampicillin and cephalosporin (86% cefuroxime) consumptions wer ... | 1989 | 2567427 |
| enteropathogenic escherichia coli serotype o111:hnt isolated from preterm neonates in nairobi, kenya. | this investigation was initiated as a consequence of several cases of diarrhea in a nursery ward for preterm babies in nairobi, kenya. ten lactose-positive colonies were isolated from the stools of each of 30 neonates, regardless of whether they had diarrhea; 229 strains were identified as escherichia coli and 65 strains were identified as klebsiella pneumoniae. six strains were lost during laboratory handling. no other bacterial, viral, or parasitic enteropathogens were identified. using synthe ... | 1989 | 2568996 |
| ecological impact of antibiotics in neonatal units. | | 1989 | 2570220 |
| the klebsiella pneumoniae pii protein (glnb gene product) is not absolutely required for nitrogen regulation and is not involved in nifl-mediated nif gene regulation. | the role of the klebsiella pneumoniae pii protein (encoded by glnb) in nitrogen regulation has been studied using two classes of glnb mutants. in class i mutants pii appears not to be uridylylated in nitrogen-limiting conditions and in class ii mutants pii is not synthesised. the effects of these mutations on expression from nitrogen-regulated promoters indicate that pii is not absolutely required for nitrogen control. furthermore the uridylylated form of pii (pii-ump) plays a significant role i ... | 1989 | 2570349 |
| the activity of the pseudomonas aeruginosa pilin promoter is enhanced by an upstream regulatory site. | recently, a comparison of the nucleotide sequences upstream of the structural pilin genes (pil) from five different isolates of pseudomonas aeruginosa identified a sequence homologous to a nifa-binding site, a positive regulatory sequence in klebsiella pneumoniae which controls the expression of the genes which fix nitrogen [pasloske et al., j. bacteriol. 170 (1988) 3738-3741]. this sequence was located between -98 and -114 bp relative to the transcription start point. to establish whether this ... | 1989 | 2572513 |
| nosocomial bacteraemia in a teaching hospital in saudi arabia. | during a period of one year, 117 episodes of nosocomial bacteraemia were documented at king khalid university hospital, an incidence of 5 per 1000 admissions. sixty-two percent were gram-negative organisms with escherichia coli, klebsiella spp., serratia spp. and pseudomonas spp. being the most frequent. staphylococcus aureus was the most common gram-positive organism isolated. the source of infection was identified in 75% of patients. intravenous lines accounted for a high proportion of cases ( ... | 1989 | 2575101 |
| an outbreak of klebsiella pneumoniae bacteraemia in five children on intravenous therapy. | | 1989 | 2575113 |
| polyclonal b-cell activation by bacteria that induce nonsuppurative sequelae. | the polyclonal b cell activation (pba) process induced by klebsiella pneumoniae k34 (klebs) and yersinia enterocolitica 03 (yers) was investigated. both heat-inactivated bacteria and their cell wall biostructures (klebsm, muriene, protein i etc.) stimulate human blood b cells to differentiate into immunoglobulin-secreting cells without prior proliferation and without t cells. klebs-activated b cells secrete mainly igm and to a lesser degree igg (mainly igg2). the pba process was regulated by cd4 ... | 1989 | 2575282 |
| [treatment of infective recurrences of chronic bronchopneumopathies with klebsiella pneumoniae extracts]. | | 1989 | 2577793 |
| sharing of antigenic determinants between the nicotinic acetylcholine receptor and proteins in escherichia coli, proteus vulgaris, and klebsiella pneumoniae. possible role in the pathogenesis of myasthenia gravis. | | 1985 | 2578213 |
| potent adjuvant action of lipopolysaccharides possessing the o-specific polysaccharide moieties consisting of mannans in antibody response against protein antigen. | it was previously reported that klebsiella o3 lipopolysaccharide (lps) exhibits extraordinarily strong adjuvant activity in augmenting antibody response against protein antigens in mice compared with other kinds of lps, for example, lps from escherichia coli o55, o111, and o127 and salmonella enteritidis. the present study was undertaken to clarify the relationship between the strong adjuvant activity in augmenting antibody response against deaggregated bovine gammaglobulin and the chemical stru ... | 1985 | 2578897 |
| klebsiella oxytoca endocarditis after transurethral resection of the prostate gland. | klebsiella oxytoca endocarditis developed in an 87-year-old man after transurethral resection of the prostate gland. he was treated for k oxytoca urinary tract infection and septicemia seven weeks before admission. fever and bacteremia persisted for ten days during therapy for endocarditis. he was treated with a combination of cefazolin and tobramycin for six weeks. despite peak serum bactericidal titers of only 1:4, the patient recovered completely and was apparently healthy at followup two yea ... | 1985 | 2579445 |
| klebsiella 'modifying factor': binding studies with hla-b27+ and b27- lymphocytes. | on the basis that extracts of some klebsiella organisms bind selectively to the lymphocytes of hla-b27+ individuals and induce the appearance of new antigens, attempts were made to detect the binding of klebsiella products to hla-b27+ and b27- lymphocytes by a number of different techniques. firstly, blocking of the binding of two different hla-b27 specific monoclonal antibodies to hla-b27+ lymphocytes has been examined following exposure of the lymphocytes to a cell-free culture filtrate from k ... | 1985 | 2579614 |
| construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the family enterobacteriaceae. | the genes encoding type 1 fimbriae of salmonella typhimurium, enterobacter cloacae, and serratia marcescens were cloned in escherichia coli. all transformants possessing recombinant plasmids were shown to be fimbriate and demonstrated mannose-sensitive hemagglutinating activity. a comparison of the physical maps of these plasmids revealed little similarity among them, although plasmids encoding type 1 fimbriae of escherichia coli and klebsiella pneumoniae appeared similar with respect to restric ... | 1985 | 2580786 |
| [demonstration of a species specific determinant of bacteroides fragilis lipopolysaccharides using monoclonal antibodies]. | the reactivity of two monoclonal antibodies to bacteroides fragilis was tested (elisa) with 37 strains of b. fragilis, 1 strain each of b. thetaiotaomicron, b. ovatus, b. vulgatus and b. eggerthii, 1 strain of fusobacterium, as well as pseudomonas aeruginosa (6 strains), e coli (8 strains), klebsiella (2 strains), yersinia enterocolitica (1 strain) and lps from salmonella minnesota. both monoclonal antibodies proved to be specific for b. fragilis. the determinants recognized by these antibodies ... | 1985 | 2581371 |
| control of klebsiella pneumoniae nif mrna synthesis. | four probes, each specific for a single nif transcript, were used for an analysis of the regulation of nif mrna synthesis. transcription of the nifla operon was repressed by nh4+ but not by amino acids, o2, or temperatures above 37 degrees c. the nifa gene product was required for the activation of transcription of the other nif operons but not nifla. synthesis of the other nif transcripts was rapidly turned off by the addition of o2, nh4+, serine, or glutamine. these regulatory effects required ... | 1985 | 2581933 |
| diagnostic considerations and interpretation of microbiological findings for evaluation of chronic prostatitis. | seventy-five patients attending a clinic for chronic prostatitis were evaluated by use of lower urinary tract localization cultures. coagulase-negative staphylococci, alpha-hemolytic streptococci, and diphtheroids were the most common isolates, but none of these organisms were pathogens, based on the absence of bacteriuria or evidence of an inflammatory response in prostatic secretions. recognized uropathogens were isolated in 12 (16%) of the 75 cases and included escherichia coli in 6 cases, en ... | 1989 | 2584375 |
| aerobic and anaerobic microbiology of biliary tract disease. | a retrospective analysis of the experiences of two military hospitals over 4 years in the recovery of organisms from biliary tract specimens was done. bacterial growth was obtained in 123 bile specimens. aerobic and facultative bacteria only were present in 59 specimens (48%), aerobic bacteria only were present in 4 specimens (3%), and mixed anaerobic and aerobic or facultative bacteria were present in 60 specimens (49%). of 286 isolates recovered, 216 were aerobic or facultative (1.8 per specim ... | 1989 | 2584384 |
| hygienic status of food handlers. | the study demonstrated bacterial species on hands and nails of food-handlers before and after hand-washing. those were staphylococcus spp., streptococcus spp., micrococcus spp., bacillus spp., diphtheroid, aeromonas hydrophila, klebsiella pneumoniae, acinetobacter, enterobacter cloacae, escherichia coli, pseudomonas spp., proteus mirabilis, serratia spp., citrobacter freundii. before hand washing, each food-handler harboured one to eight bacterial species. after hand-washing (eight with water fr ... | 1989 | 2584903 |
| chronic bronchitis. iv--antibody titres to bacterial antigens during acute exacerbations. | in 49 patients of chronic bronchitis with acute exacerbation, serum antibody titres were estimated against the respective pathogen isolated and cultured from sputum of individual patients. antibody titres to klebsiella and staphylococcus coagulase positive organisms were found in 28 and 25 patients respectively. paired serum samples from 21 patients showed rising antibody titres in 17 of them, whereas the antibody titres fell in the remaining 4 patients. notably, only 2 patients demonstrated a f ... | 1989 | 2585335 |
| mycotic aneurysms of the suprarenal abdominal aorta: prolonged survival after in situ aortic and visceral reconstruction. | necrotizing infection of the arterial wall causes rupture and false ("mycotic") aneurysm formation, with a very poor prognosis if untreated. cure can be achieved by surgical drainage and debridement, with restoration of arterial continuity through uncontaminated tissues. the dilemma of applying these principles to the treatment of mycotic aneurysms of the suprarenal aorta is that no remote or extraanatomic routes are available to maintain perfusion to the viscera. we report the first case of kle ... | 1989 | 2585652 |
| [immunotherapy with solco urovac vaccine in chronic genitourinary infection]. | twenty five patients with chronic urogenital infections (cystitis, pyelitis and prostatitis) were treated with solco urovak infection which is active against escherichia coli, proteus, klebsiella and streptococcus fecalis organisms. this microbial flora has been isolated from these patients and proved resistant to the conventional antibacterial agents. the vaccine was very well tolerated, gave no side reactions, but transient short lasting rise of the temperature to 38 degrees c. the bacteriolog ... | 1989 | 2585977 |
| biostin (ru-41740) does not impair antipyrine metabolism in healthy volunteers. | | 1989 | 2586200 |
| a possible correlation between histological changes in regional subcutaneous tissue induced by bacterial lipopolysaccharides and their adjuvant activities. | previously it was demonstrated that klebsiella pneumoniae o3 lipopolysaccharide (ko3 lps) exhibited much stronger adjuvant action on antibody response to subcutaneously (s.c.) injected sheep red blood cells or deaggregated bovine serum albumin than did other kinds of lps, the r-form lps lacking the o-specific polysaccharide chain of ko3 lps (r-lps), and the lipid a fractionated from ko3 lps. we compared histological changes in the regional subcutaneous tissues of mice injected subcutaneously (s. ... | 1989 | 2586346 |
| ciprofloxacin in patients with bacteremic infections. the spanish group for the study of ciprofloxacin. | the efficacy and safety of ciprofloxacin in the treatment of 68 episodes of bacteremia were studied. patients were treated intravenously (30 cases), orally (13 cases), or with sequential intravenous/oral therapy (25 cases). intravenous doses ranged from 200 to 400 mg per day and oral doses ranged from 1,000 to 1,500 mg per day. according to the criteria of mccabe and jackson, 39 cases had nonfatal and 29 had ultimately fatal underlying diseases. the clinical condition of patients at the start of ... | 1989 | 2589366 |
| effect of the abscess environment on the antimicrobial activity of ciprofloxacin. | the present studies were conducted to identify factors in human purulent material that might limit or enhance the activity of ciprofloxacin against bacteria causing suppurative infection. ciprofloxacin, imipenem, and ampicillin were tested with regard to binding or inactivation by pus. the bactericidal activity of ciprofloxacin and imipenem were tested against escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, or staphylococcus aureus in human pus with a ph of 6.0 incubated at 37 d ... | 1989 | 2589367 |
| treatment of sepsis in patients with neoplastic diseases with intravenous ciprofloxacin. | hospitalized patients with sepsis caused by ciprofloxacin-susceptible organisms were treated with intravenous ciprofloxacin 300 mg every 12 hours. patients were followed closely for side effects and adverse reactions as well as for their response to therapy. culture specimens and laboratory parameters including serum chemistry profile, complete blood cell count, and urinalysis were obtained before, during (every four to five days), and after ciprofloxacin therapy. antimicrobial susceptibility of ... | 1989 | 2589376 |
| comparative in vitro activity and beta-lactamase stability of fk482, a new oral cephalosporin. | fk482 is an oral aminothiazolyl hydroxyimino cephalosporin with a c-3 vinyl group. its activity was compared with those of cephalexin, cefuroxime, cefixime, and amoxicillin-clavulanate. fk482 inhibited 90% of staphylococcus aureus isolates at 1 micrograms/ml and 90% of streptococcus pyogenes, streptococcus agalactiae, and streptococcus pneumoniae isolates at less than or equal to 0.012 micrograms/ml, superior to cephalexin and cefuroxime and similar to cefixime. it did not inhibit oxacillin-resi ... | 1989 | 2589845 |
| combination effect of recombinant human interleukin-1 alpha with antimicrobial agents. | combination effects of recombinant human interleukin-1 alpha with ceftazidime, moxalactam, gentamicin, enoxacin, amphotericin b, miconazole, or an immunoglobulin preparation were evaluated in systemic infections with pseudomonas aeruginosa, klebsiella pneumoniae, and candida albicans in normal mice and systemic infection with p. aeruginosa in mice with leukopenia induced by preadministration of cyclophosphamide. synergistic effects were generally observed at interleukin-1 alpha doses as low as 1 ... | 1989 | 2589847 |
| carbohydrate fermentation by gut microflora in preterm neonates. | to study organic acid excretion, urine was collected from 52 preterm infants at weekly intervals and analysed by capillary gas chromatography-mass spectrometry. twelve of 22 babies born before 33 weeks' gestation excreted 2,3-butanediol, as did six born between 33 and 36 weeks. six very immature babies also excreted acetoin, the metabolic precursor of the diol. other products derived from carbohydrate included methylmalonic and ethylmalonic acids in one baby, and d-lactic acid in five. acetoin h ... | 1989 | 2589871 |
| [effect of sisomicin on intestinal microflora and colonization resistance in rats and their offspring]. | in experiments with wistar female rats it was shown that after administration in the form of inhalations (10.87 mg/m3) sisomicin was present in the animal feces in a concentration of 26.6 micrograms/g. prolonged exposure to the antibiotic during the pregnancy term led to changes in microbiocenosis of the animal large intestine which was observed even a month after the delivery. the progeny of the animals was characterized by the presence of significant numbers of aerobic bacteria resistant to si ... | 1989 | 2589899 |
| survival of and plasmid stability in pseudomonas and klebsiella spp. introduced into agricultural drainage water. | cell survival and plasmid stability in pseudomonas fluorescens r2f and pseudomonas putida cym 318 containing respectively, plasmid rp4 and prk2501, and klebsiella aerogenes nctc 418 harboring plasmid pbr322 were studied in sterile and nonsterile agricultural drainage water under both aerobic and anaerobic conditions and in the absence and presence of added nutrients. both pseudomonas strains survived well in sterile drainage water incubated aerobically, with or without added nutrients. however, ... | 1989 | 2590305 |
| the source of campylobacter pylori. | sera from 98 abattoir workers were tested for igg as well as for iga to campylobacter pylori, c. jejuni and klebsiella. clerical workers had significantly lower c. pylori and c. jejuni antibody levels than any of the groups in direct contact with freshly cut animal parts. no difference was found for antibodies to klebsiella. twenty-nine non-clerical workers with high igg antibody levels against c. pylori consented to upper gastrointestinal endoscopy. c. pylori associated gastritis was found in a ... | 1989 | 2590720 |
| increased urinary nitrosamine excretion in patients with urinary diversions. | tumor development at the site of ureterointestinal anastomosis is a recognized complication in patients with continent urinary diversions. aerobic cultures of rectal urine samples from 30 patients with urinary diversions (26 ureterosigmoidostomies, two colon conduits, one ileal conduit and a gersuny bladder) showed a complex bacterial flora containing nitrate-reducing organisms (escherichia coli, proteus and klebsiella spp.). in comparison to normal bladder urine samples from control volunteers ... | 1989 | 2591028 |
| antibiotic resistance in australia with special reference to sulbactam/ampicillin. | a survey of antibiotic resistance in australian states was undertaken by the microbiology quality assurance program of the royal college of pathologists of australasia. data were obtained from hospitals and private pathology laboratories serving both in-patients and out-patients at community hospitals. the study showed that resistance varied from state to state; it was highest in the eastern states of new south wales, victoria, and queensland, and lowest in tasmania and western australia. in sou ... | 1989 | 2591174 |
| identification of species and capsular types of klebsiella clinical isolates, with special reference to klebsiella planticola. | in the 77 reference strains for klebsiella k types, there are 17 strains (22.1%) of klebsiella planticola, 6 strains (7.8%) of klebsiella oxytoca, 1 strain (1.3%) of klebsiella terrigena, and 53 strains (68.8%) of klebsiella pneumoniae. the species k. planticola, which was originally isolated from botanical and aquatic environments and hence thus named, was also identified at high incidence (81 strains, 18.5%) among the 439 recent clinical isolates of klebsiella species. among these k. planticol ... | 1989 | 2593874 |
| recombinant dna research; proposed actions under guidelines. national institutes of health, phs, dhhs. | this notice sets forth proposed actions to be taken under the national institutes of health (nih) guidelines for research involving recombinant dna molecules. interested parties are invited to submit comments concerning these proposals. these proposals will be considered by the recombinant dna advisory committee (rac) at its meeting on october 6, 1989. after consideration of these proposals and comments by the rac, the acting director of the national institutes of health will issue decisions in ... | 1989 | 2595019 |
| a contribution to the situation of antibiotic resistance in abeokuta (ogunstate) and minna (nigerstate) in nigeria. | after testing various chemotherapeutics the following conclusions could be drawn: pseudomonas was sensitive only to gentamicin. gentamicin, aminopenicilline + calvulanacid and cefoxitin were 100% effective against e. coli. gentamicin also proved effective against enterobacter (83%). cefoxitin, aminopenicillin + clavulanacid, gentamicin and trimethoprim + sulfonamide were effective against klebsiella. concerning proteus sp., cefoxitin showed best results (100%). acinetobacter was 100% inhibited b ... | 1989 | 2599386 |
| analysis of blood-culture isolates from neonates of a rural hospital. | all blood culture reports obtained from newborns admitted in a neonatal care unit during 1983-87 were reviewed to determine the current bacteriological profile of neonatal septicemia. a total of 1160 neonates of high risk category or having clinical features suggestive of septicemia were subjected to blood culture study during this period. the culture positivity rate was 73%. of these, 24.9% cultures were polymicrobial. among the 1059, growths obtained, 60.1% were gram negative, with klebsiella, ... | 1989 | 2599614 |
| urethral obstruction in a male llama. | an 8-year-old sexually intact male llama was euthanatized because of obstruction of the penile portion of the urethra and rupture of the urinary bladder. clinical signs of obstruction included anorexia, lethargy, teeth grinding, straining to defecate, inability to urinate, and tense abdomen. laboratory findings included anemia, leukocytosis with left shift, high serum urea nitrogen, creatinine, and phosphorus concentrations, and low serum chloride concentration. necropsy revealed ruptured urinar ... | 1989 | 2599947 |
| pattern of infection in hematologic malignancies: an indian experience. | ninety-nine consecutive patients who received cytotoxic therapy for acute leukemia were retrospectively studied to determine the pattern of infection at the tata memorial hospital, bombay, india. in all, 224 infective episodes occurred in these patients. bacterial infection was the commonest type, accounting for 152 (67.9%) of 224 infective episodes, followed by fungal and viral infections (15.6% and 14.3%, respectively). gram-negative organisms (pseudomonas and klebsiella) were the commonest ba ... | 1989 | 2602780 |
| ticarcillin/clavulanate in severe infections in patients with varying renal function. | the therapeutic efficacy of ticarcillin/clavulanate was assessed in 71 patients with severe infections: 38 acute pyelonephritis, 16 septicaemia and 19 miscellaneous infections. the patients were classified according to their renal function in: group a, normal (16 cases); b, mild renal impairment (ri) with creatinine clearance (clcr) between 80 and 40 ml/min (18 cases); c, moderate ri with clcr between 40 and 15 ml/min (12 cases); d, severe ri with (clcr) between 15 and 5 ml/min (13 cases) and e, ... | 1989 | 2606815 |
| efficacy of clavulanate-potentiated antibiotics against bacteroides species and artificially associated cultures of aerobes and anaerobes. | clavulanic acid is known to potentiate the activity of amoxycillin and ticarcillin against beta-lactamase-producing bacteroides species. in order to assess the usefulness of the clavulanate antibiotics in mixed infections with aerobes and anaerobes, artificially associated cultures of clinically significant bacteroides species and facultative anaerobes (escherichia coli, klebsiella pneumoniae, haemophilus influenzae, enterococcus faecalis, staphylococcus spp. and streptococcus spp.) were tested. ... | 1989 | 2606820 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1986). iii. secular changes in susceptibility]. | changes in the susceptibility of various infectious microorganisms to antimicrobial agents from 1982 to 1986 were evaluated. the microorganisms investigated were escherichia coli, klebsiella spp., citrobacter spp., enterobacter spp., proteus spp., serratia marcescens and pseudomonas aeruginosa isolated from patients with urinary tract infections. we compared susceptibilities of microorganisms obtained from simple urinary tract infections with those from complicated infections with or without ind ... | 1989 | 2607604 |
| clinical efficacy of sulbactam/ampicillin in the treatment of moderately severe bacterial infections. | 20 patients with moderately severe bacterial infections were studied to determine the clinical efficacy and safety of parenteral sulbactam/ampicillin. there were 9 female and 11 male patients. their mean age was 51 years. 8 patients had pneumonia, 5 urinary tract infection, 4 cellulitis of the leg and 3 had pustular tonsillitis. 85% of patients had resolution of fever and symptoms within 48 hours of commencing treatment. 95% had successful treatment outcome. the organisms isolated included e. co ... | 1989 | 2609176 |
| a re-investigation of the structure of the capsular polysaccharide of klebsiella k10. | the capsular polysaccharide of klebsiella k10 was investigated by methylation analysis and 1h-n.m.r. spectroscopy, and the deacetylated bacteriophage-degraded polysaccharide by 1d- and 2d-n.m.r. spectroscopy. the repeating unit was shown to be a branched hexasaccharide (see text). oac substituents were located on the terminal and 2-linked galactopyranosyl residues by prehm methylation of a low-molecular-weight fraction obtained by bacteriophage degradation. | 1989 | 2611780 |
| ceftriaxone monotherapy for severe bacteremic infections. spanish ceftriaxone study group. | we tested the efficacy of a single daily dose of ceftriaxone (active ingredient of rocephin) for the treatment of severe bacteremic infections in 125 non-neutropenic adult patients. a single daily dose of ceftriaxone ranging from 1 to 4 g was given. surgical procedures were performed if needed. seventy-six (60.8%) were males and bacteremia was nosocomially acquired in 45 (36%). microbiologically proven bacteremia was demonstrated in all patients. the most common microorganisms isolated were esch ... | 1989 | 2612237 |