| diversity, epidemiology, and genetics of class d beta-lactamases. | class d beta-lactamase-mediated resistance to beta-lactams has been increasingly reported during the last decade. those enzymes also known as oxacillinases or oxas are widely distributed among gram negatives. genes encoding class d beta-lactamases are known to be intrinsic in many gram-negative rods, including acinetobacter baumannii and pseudomonas aeruginosa, but play a minor role in natural resistance phenotypes. the oxas (ca. 150 variants reported so far) are characterized by an important ge ... | 2010 | 19721065 |
| neonatal enteral feeding tubes as loci for colonisation by members of the enterobacteriaceae. | the objective of this study was to determine whether neonatal nasogastric enteral feeding tubes are colonised by the opportunistic pathogen cronobacter spp. (enterobacter sakazakii) and other enterobacteriaceae, and whether their presence was influenced by the feeding regime. | 2009 | 19723318 |
| [epidemiology of enterobacteriaceae causing bloodstream infections in neonatal intensive care unit patients]. | an increasing number of neonates require intensive care, and this population has a high risk of developing infections. the enterobacteriaceae most commonly related to healthcare-associated infection in neonatal intensive care units (nicu) are escherichia coli, klebsiella spp and enterobacter cloacae. | 2010 | 19726111 |
| international survey of cronobacter sakazakii and other cronobacter spp. in follow up formulas and infant foods. | a coordinated survey for cronobacter and related organisms in powdered infant formula, follow up formula and infant foods was undertaken by 8 laboratories in 7 countries in recognition of and in response to the data needs identified in an fao/who call for data in order to develop global risk management guidance for these products. the products (domestic and imported) were purchased from the local market and were categorised according to their principle ingredients. a total of 290 products were a ... | 2009 | 19729216 |
| identification of burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody. | the genus burkholderia includes many bacteria that cause serious human infections. as is the case with other gram-negative bacteria, burkholderia species produce lps, which is an abundant component of the bacterial cell surface. burkholderia cepacia complex (bcc) bacteria (which include at least 17 separate species) produce lps structures that are quite different. in an attempt to determine the degree of lps epitope variation among bcc species, a mab was produced, designated 5d8, specific for th ... | 2010 | 19729457 |
| structural and biochemical characterization of the interaction between kpc-2 beta-lactamase and beta-lactamase inhibitor protein. | kpc beta-lactamases hydrolyze the "last resort" beta-lactam antibiotics (carbapenems) used to treat multidrug resistant infections and are compromising efforts to combat life-threatening gram-negative bacterial infections in hospitals worldwide. consequently, the development of novel inhibitors is essential for restoring the effectiveness of existing antibiotics. the beta-lactamase inhibitor protein (blip) is a competitive inhibitor of a number of class a beta-lactamases. in this study, we chara ... | 2009 | 19731932 |
| evaluation of a novel real-time pcr test based on the ssra gene for the identification of group b streptococci in vaginal swabs. | despite the implementation of prevention guidelines, early-onset group b streptococci (gbs) disease remains a cause of neonatal morbidity and mortality worldwide. strategies to identify women who are at risk of transmitting gbs to their infant and the administration of intrapartum antibiotics have greatly reduced the incidence of neonatal gbs disease. however, there is a requirement for a rapid diagnostic test for gbs that can be carried out in a labour ward setting especially for women whose gb ... | 2009 | 19732424 |
| in vitro activity of tigecycline and comparator agents against a global collection of gram-negative and gram-positive organisms: tigecycline evaluation and surveillance trial 2004 to 2007. | the tigecycline evaluation and surveillance trial began in 2004 to monitor the in vitro activity of tigecycline and comparator agents against a global collection of gram-negative and gram-positive pathogens. against gram negatives (n = 63 699), tigecycline mic(90)'s ranged from 0.25 to 2 mg/l for escherichia coli, haemophilus influenzae, acinetobacter baumannii, klebsiella oxytoca, enterobacter cloacae, klebsiella pneumoniae, and serratia marcescens (but was > or =32 for pseudomonas aeruginosa). ... | 2009 | 19733459 |
| catabolism of raffinose, sucrose, and melibiose in erwinia chrysanthemi 3937. | erwinia chrysanthemi (dickeya dadantii) is a plant pathogenic bacterium that has a large capacity to degrade the plant cell wall polysaccharides. the present study reports the metabolic pathways used by e. chrysanthemi to assimilate the oligosaccharides sucrose and raffinose, which are particularly abundant plant sugars. e. chrysanthemi is able to use sucrose, raffinose, or melibiose as a sole carbon source for growth. the two gene clusters scrkyabr and rafrba are necessary for their catabolism. ... | 2009 | 19734309 |
| isolation of faecal coliform bacteria from the american alligator (alligator mississippiensis). | to determine whether american alligators (alligator mississippiensis) are an unrecognized poikilothermic source of faecal coliform and/or potential pathogenic bacteria in south carolina's coastal waters. | 2010 | 19735329 |
| nosocomial spread of colistin-only-sensitive sequence type 235 pseudomonas aeruginosa isolates producing the extended-spectrum beta-lactamases ges-1 and ges-5 in spain. | the mechanisms responsible for the increasing prevalence of colistin-only-sensitive (cos) pseudomonas aeruginosa isolates in a spanish hospital were investigated. pulsed-field gel electrophoresis revealed that 24 (50%) of the studied isolates belonged to the same clone, identified as the internationally spread sequence type 235 (st235) through multilocus sequence typing. in addition to several mutational resistance mechanisms, an integron containing seven resistance determinants was detected. re ... | 2009 | 19738007 |
| primary structure and antibacterial activity of chicken bone marrow-derived beta-defensins. | three biologically active beta-defensins were purified by chromatography from chicken bone marrow extract: avian beta-defensin 1 (avbd1), avbd2, and the newly isolated beta-defensin avbd7. mass spectrometry analyses showed that bone marrow-derived avbd1, -2, and -7 peptides were present as mature peptides and revealed posttranslational modifications for avbd1 and avbd7 in comparison to their in silico-predicted amino acid sequences. tandem mass spectrometry analysis using the nanoelectrospray-qu ... | 2009 | 19738012 |
| in vitro activity of nemonoxacin, a novel nonfluorinated quinolone, against 2,440 clinical isolates. | the in vitro activity of nemonoxacin (tg-873870), a novel nonfluorinated quinolone, was tested against 2,440 clinical isolates. nemonoxacin was at least fourfold more active than levofloxacin and moxifloxacin against most gram-positive cocci tested (shown by the following mic(90)/range [microg/ml] values; community-associated methicillin [meticillin]-resistant staphylococcus aureus, 0.5/0.015 to 2; staphylococcus epidermidis, 0.5/0.015 to 4 for methicillin-susceptible staphylococci and 2/0.12 to ... | 2009 | 19738018 |
| extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil. | extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ... | 2009 | 19739000 |
| infections with vim-1 metallo-{beta}-lactamase-producing enterobacter cloacae and their correlation with clinical outcome. | the aim of this study was to ascertain the incidence and clinical significance of metallo-beta-lactamases among enterobacter strains isolated from patients with nosocomial infections. we prospectively collected data on patients with enterobacter infection during a 13-month period. all of the strains were investigated for antibiotic susceptibility, the presence and expression of metallo-beta-lactamases, and clonality. of 29 infections (11 involving the urinary tract, 7 pneumonias, 3 skin/soft tis ... | 2009 | 19741074 |
| plant-associated bacterial degradation of toxic organic compounds in soil. | a number of toxic synthetic organic compounds can contaminate environmental soil through either local (e.g., industrial) or diffuse (e.g., agricultural) contamination. increased levels of these toxic organic compounds in the environment have been associated with human health risks including cancer. plant-associated bacteria, such as endophytic bacteria (non-pathogenic bacteria that occur naturally in plants) and rhizospheric bacteria (bacteria that live on and near the roots of plants), have bee ... | 2009 | 19742157 |
| clinical course and spectrum of intensive care unit patients reactivating herpes simplex-1 virus: a retrospective analysis. | herpes simplex-1 virus (hsv-1) reactivation in the respiratory tract is common in intensive care unit (icu) patients. however, susceptible icu populations are poorly defined. clinical recognition of hsv infection of the respiratory tract is difficult and the impact of such reactivation is not understood. | 2008 | 19742269 |
| egfrviii-targeted vaccination therapy of malignant glioma. | given the highly infiltrative growth pattern of malignant glioma and the lack of specificity associated with currently available treatment regimens, alternative strategies designed to eradicate cancer cells while limiting collateral toxicity in normal tissues remain a high priority. to this end, the development of specific immunotherapies against targeted neoplastic cells represents a promising approach. the epidermal growth factor receptor class iii variant (egfrviii), a constitutively activate ... | 2009 | 19744042 |
| in vitro susceptibilities of aerobic and facultative anaerobic gram-negative bacilli from patients with intra-abdominal infections worldwide from 2005-2007: results from the smart study. | smart (study for monitoring antimicrobial resistance trends) is an ongoing multiyear surveillance study to monitor worldwide antimicrobial resistance trends among aerobic and facultative anaerobic gram-negative bacilli (gnb) isolated from intra-abdominal infections. during 2005-2007, a total of 255 laboratories from 37 countries in five global regions collected intra-abdominal gnb for antimicrobial susceptibility testing using broth microdilution according to clinical and laboratory standards in ... | 2009 | 19748234 |
| ceftobiprole, a broad-spectrum cephalosporin with activity against methicillin-resistant staphylococcus aureus (mrsa). | | 2008 | 19750059 |
| expression of bacterial genes in transgenic tobacco: methods, applications and future prospects. | tobacco is the most commonly used plant for expression of transgenes from a variety of organisms, because it is easily grown and transformed, it provides abundant amounts of fresh tissue and has a well-established cell culture system. many bacterial proteins involved in the synthesis of commercial products are currently engineered for production in tobacco. bacterial enzymes synthesized in tobacco can enhance protection against abiotic stresses and diseases, and provide a system to test applied ... | 2007 | 19750137 |
| novel beta-lactamase-random peptide fusion libraries for phage display selection of cancer cell-targeting agents suitable for enzyme prodrug therapy. | novel phage-displayed random linear dodecapeptide (x(12)) and cysteine-constrained decapeptide (cx(10)c) libraries constructed in fusion to the amino-terminus of p99 beta-lactamase molecules were used for identifying beta-lactamase-linked cancer cell-specific ligands. the size and quality of both libraries were comparable to the standards of other reported phage display systems. using the single-round panning method based on phage dna recovery, we identified several beta-lactamase fusion peptide ... | 2010 | 19751096 |
| the antibacterial properties of malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. | antibiotic resistance of bacteria is on the rise, thus the discovery of alternative therapeutic agents is urgently needed. honey possesses therapeutic potential, including wound healing properties and antimicrobial activity. although the antimicrobial activity of honey has been effectively established against an extensive spectrum of microorganisms, it differs depending on the type of honey. to date, no extensive studies of the antibacterial properties of tualang (koompassia excelsa) honey on wo ... | 2009 | 19754926 |
| the olive fly endosymbiont, "candidatus erwinia dacicola," switches from an intracellular existence to an extracellular existence during host insect development. | as polyphagous, holometabolous insects, tephritid fruit flies (diptera: tephritidae) provide a unique habitat for endosymbiotic bacteria, especially those microbes associated with the digestive system. here we examine the endosymbiont of the olive fly [bactrocera oleae (rossi) (diptera: tephritidae)], a tephritid of great economic importance. "candidatus erwinia dacicola" was found in the digestive systems of all life stages of wild olive flies from the southwestern united states. pcr and micros ... | 2009 | 19767463 |
| nosocomial transmission of highly resistant microorganisms on a spinal cord rehabilitation ward. | to study the mechanism of nosocomial transmission of highly resistant microorganisms (hrmos). | 2009 | 19777864 |
| performance of vitek-2 compact and overnight microscan panels for direct identification and susceptibility testing of gram-negative bacilli from positive fan bact/alert blood culture bottles. | we describe the reliability of the vitek-2 compact and overnight microscan panels for direct identification and susceptibility testing from the bact/alert blood culture system when using fan (fa and fn) bottles. a simple procedure, in two centrifugation steps, was designed to remove the charcoal particles present in fa and fn bottles. a total of 113 positive blood cultures showing gram-negative rods were investigated. enterobacteriaceae were isolated in 104 cases, and pseudomonas aeruginosa in n ... | 2010 | 19778301 |
| introduction of ertapenem into a hospital formulary: effect on antimicrobial usage and improved in vitro susceptibility of pseudomonas aeruginosa. | after ertapenem was added to the formulary of a 344-bed community teaching hospital, we retrospectively studied its effect on antimicrobial utilization and on the in vitro susceptibility of various antimicrobial agents against pseudomonas aeruginosa. three study periods were defined as preintroduction (months 1 to 9), postintroduction but before the autosubstitution of ertapenem for ampicillin-sulbactam (months 10 to 18), and after the policy of autosubstitution (months 19 to 48) was initiated. ... | 2009 | 19786596 |
| acinetobacter baumannii-associated skin and soft tissue infections: recognizing a broadening spectrum of disease. | acinetobacter baumannii is gaining importance as a cause of nosocomial infections, but its role in skin and soft tissue infection (ssti) is not well defined. as a result of the outbreak of a. baumannii occurring in military personnel in iraq and afghanistan, reports of severe wound infections and ssti caused by this pathogen are increasing in frequency. | 2010 | 19788383 |
| diagnosis of endotoxemia with gram-negative bacteremia is bacterial species dependent: a meta-analysis of clinical studies. | endotoxemia is undetectable for up to 60% of cases of bacteremia caused by gram-negative (gn) species, a discordance attributed to the limitations of the limulus assay for endotoxemia. the lipid a structure of the endotoxin molecule is critical for the sensing of gn bacteria by the host immune system although not so for sensing by the limulus assay. the lipid a structure of commensal enterobacteriaceae is hexa-acyl, whereas non-enterobacteriaceae have a broader range of structures. by using a pr ... | 2009 | 19794055 |
| [is pneumatic sample transport system also a carrier for microorganisms?]. | the purpose of this study was to evaluate the possible infection and contamination risk of the pneumatic system used in our hospital and to establish essential infection control measures. the study was conducted in a quaternary health care center with 1.000 bed capacity. a total of 614 specimens were taken 2 times weekly from the pneumatic transport system and its carriers at 22 wards, 5 intensive care units, 3 laboratories, 2 blood taking units, and pharmacy. samples were also obtained from the ... | 2009 | 19795620 |
| kinetic and theoretical study of the chalcones as inhibitors of beta-lactamase enzyme. | staphyloccoccus aureus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii, enterobacter cloacae and more bacteria have shown resistance to antibiotics in colombia, therefore, resistance to antibiotics is a problem that is on increase in colombia. the resistance mechanism to penicillin antibiotics in these bacteria is the expression of beta-lactamase enzyme. in order to use the penicillin antibiotics which are still effective against them, we had evaluated 1 ... | 2009 | 19799548 |
| antibacterial activity of ceftazidime (gr 20263) against routine hospital isolates and highly resistant strains of enterobacteriaceae and pseudomonas aeruginosa. | ceftazidime was found to be highly active against routine hospital isolates of strains of enterobacteriaceae such as escherichia coli, klebsiella aerogenes, enterobacter cloacae and proteus mirabilis, with the mean mics varying between 0.11 and 0.75 mg/l. it had marked activity against pseudomonas aeruginosa with a mean mic of 1.8 mg/l which was comparable to tobramycin (11 mg/l) and superior to other aminoglycosides, penicillins and cefotaxime. although ceftazidime was considerably more active ... | 1981 | 19802975 |
| ceftazidime, a pseudomonas-active cephalosporin: in-vitro antimicrobial activity evaluation including recommendations for disc diffusion susceptibility tests. | the in-vitro qualities of ceftazidime, as compared to other beta-lactams and three other antimicrobials (amikacin, gentamicin and chloramphenicol), were evaluated in a multilaboratory, multiphasic study in the united states. a total of 12,986 recent clinical isolates were tested by reference dilution methods in six medical centres over 45-60 days. ceftazidime was superior to the comparison cephalosporin (cefamandole) and comparable in spectrum and activity to gentamicin against enterobacteriacea ... | 1981 | 19802985 |
| the in-vivo antibacterial activity of ceftazidime (gr 20263)--a comparison with other new beta-lactam antibiotics and gentamicin. | ceftazidime was compared with cefotaxime, ceftizoxime, cefmenoxime, cefotiam, cefoperazone, moxalactam, piperacillin, carbenicillin, mezlocillin, cefsulodin and the aminoglycoside antibiotic gentamicin in a series of mouse protection tests. ceftazidime, together with the other cephalosporin antibiotics and moxalactam were equally effective against infections caused by staphylococcus aureus with ed50 values ranging from 3.5 to 25 mg/kg. gentamicin was the most active antibiotic with ed50 values o ... | 1981 | 19802994 |
| ceftazidime in gram-negative infections: three case reports. | three patients with serious infections due to gram-negative bacilli were treated with ceftazidime: a 52-year-old woman with enterobacter cloacae meningitis, a 33-year-old woman with post-traumatic pseudomonas aeruginosa mediastinitis and a 68-year-old man with myelomatosis and ps. aeruginosa pneumonia. in all three cases the use of ceftazidime, in combination with gentamicin, resulted in clinical improvement associated with rapid clearing of the organism from infected sites. no haematological or ... | 1981 | 19803006 |
| behaviour of ceftazidime towards beta-lactamases. | from escherichia coli, enterobacter cloacae, proteus morganii and pseudomonas aeruginosa, but the geometric mean of the inhibition constant ki used to measure this interaction was 120 and almost 700 times higher with ceftazidime than with cefotaxime and cefuroxime, respectively. two other beta-lactamases isolated from pr. vulgaris and klebsiella oxytoca also showed little or no interaction with ceftazidime. a complementary microbiological technique, the "double-disc" technique, indicated that ce ... | 1981 | 19810176 |
| emerging infections in burns. | patients who suffer severe burns are at higher risk for local and systemic infections. in recent years, emerging resistant pathogens have forced burn care providers world wide to search for alternative forms of treatment. multidrug-resistant staphylococcus aureus, pseudomonas aeruginosa, acinetobacter spp., and various fungal strains have been the major contributors to the increase in morbidity and mortality rates. multi-drug-resistant s. aureus remains the major cause of gram-positive burn woun ... | 2009 | 19810827 |
| bacterial sepsis after prostate biopsy--a new perspective. | to determine the incidence of sepsis following transrectal ultrasound (trus)-guided prostate biopsy at our center. | 2009 | 19815258 |
| bacterial tracheobronchitis. a rare cause of adult airway stenosis. | bacterial tracheobronchitis is a rare cause of airway stenosis in adults. this report describes a 73-year-old woman with a recent history of polysialadenitis, who presented with severe airway obstruction due to infection and stenosis of tracheal and bronchial tissue. tissue culture of the bronchial mucosa showed growth of methicillin resistant staphylococcus epidermidis (mrse). sputum culture showed growth of mrse, pseudomonas aeruginosa, enterobacter cloacae and enterococcus faecalis; the same ... | 2009 | 19818053 |
| transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges. | insect-borne diseases cause significant human morbidity and mortality. current control and preventive methods against vector-borne diseases rely mainly on insecticides. the emergence of insecticide resistance in many disease vectors highlights the necessity to develop new strategies to control these insects. vector transgenesis and paratransgenesis are novel strategies that aim at reducing insect vectorial capacity, or seek to eliminate transmission of pathogens such as plasmodium sp., trypanoso ... | 2010 | 19819346 |
| transgenesis and paratransgenesis to control insect-borne diseases: current status and future challenges. | insect-borne diseases cause significant human morbidity and mortality. current control and preventive methods against vector-borne diseases rely mainly on insecticides. the emergence of insecticide resistance in many disease vectors highlights the necessity to develop new strategies to control these insects. vector transgenesis and paratransgenesis are novel strategies that aim at reducing insect vectorial capacity, or seek to eliminate transmission of pathogens such as plasmodium sp., trypanoso ... | 2010 | 19819346 |
| antibacterial-resistant pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. | treatment of infectious diseases becomes more challenging with each passing year. this is especially true for infections caused by the opportunistic pathogen pseudomonas aeruginosa, with its ability to rapidly develop resistance to multiple classes of antibiotics. although the import of resistance mechanisms on mobile genetic elements is always a concern, the most difficult challenge we face with p. aeruginosa is its ability to rapidly develop resistance during the course of treating an infectio ... | 2009 | 19822890 |
| misdiagnosed esophageal perforation treated with endoscopic stent placement: a case report. | esophageal perforation has a high rate of mortality. many strategies have been advocated for its management. therapeutic options are surgical repair or resection, endoscopic placement of self-expandable metallic stents or, in selected cases, conservative management.we describe a case of a 75-year-old man admitted to our hospital for forceful vomiting since 24 hours. the patient was treated with endoscopic placement of a covered self expandable metallic stent. although the late diagnosis delayed ... | 2009 | 19829835 |
| intraspecific variation and interspecific differences in the bacterial and fungal assemblages of blue tit (cyanistes caeruleus) and great tit (parus major) nests. | although interest in the relationship between birds and microorganisms is increasing, few studies have compared nest microbial assemblages in wild passerines to determine variation within and between species. culturing microorganisms from blue tit (cyanistes caeruleus) and great tit (parus major) nests from the same study site demonstrated diverse microbial communities with 32 bacterial and 13 fungal species being isolated. dominant bacteria were pseudomonas fluorescens, pseudomonas putida, and ... | 2010 | 19830477 |
| generation of b cell memory to the bacterial polysaccharide alpha-1,3 dextran. | b1b b cells generate a novel form of memory and provide ab mediated-protection to persisting bacterial pathogens. to understand how b1b b cells establish memory to polysaccharide ags, we studied an oligoclonal b cell response to alpha-1,3 dextran (dex) expressed on enterobacter cloacae. b cells specific for dex enrich in the marginal zone (mz) and b1b b cell populations. after e. cloacae immunization, mz b cells were responsible for the generation of initial peak dex-specific ab titers, whereas, ... | 2009 | 19841173 |
| seasonal variation in escherichia coli bloodstream infection: a population-based study. | seasonal variation in the rates of infection with certain gram-negative organisms has been previously examined in tertiary-care centres. we performed a population-based investigation to evaluate the seasonal variation in escherichia coli bloodstream infection (bsi). we identified 461 unique patients in olmsted county, minnesota, from 1 january 1998 to 31 december 2007, with e. coli bsi. incidence rates (ir) and ir ratios were calculated using rochester epidemiology project tools. multivariable p ... | 2009 | 19845704 |
| [evolution of third-generation cephalosporin resistance in enterobacteriaceae from 2000 to 2008 in a perpignan hospital]. | the aim of this work was to evaluate the evolution of enterobacteriaceae resistance to third generation cephalosporin (3cg) from 2000 to 2008 at perpignan hospital. were observed: the percentage of strains isolated from short stay wards, intensive care unit and medium and long-term care facility. the percentage of strains isolated from: urine, suppuration, tracheal aspiration, and blood have been evaluated. the proportion of escherichia coli (e. coli) strains among the enterobacteriaceae strains ... | 2010 | 19854588 |
| klebsiella pneumoniae acrab efflux pump contributes to antimicrobial resistance and virulence. | respiratory infections caused by klebsiella pneumoniae are characterized by high rates of mortality and morbidity. management of these infections is often difficult, due to the high frequency of strains that are resistant to multiple antimicrobial agents. multidrug efflux pumps play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. in the present study, we investigated the role of the k. pneumoniae acrrab operon in antimicrobial resistance and virulence by using ... | 2010 | 19858254 |
| klebsiella pneumoniae acrab efflux pump contributes to antimicrobial resistance and virulence. | respiratory infections caused by klebsiella pneumoniae are characterized by high rates of mortality and morbidity. management of these infections is often difficult, due to the high frequency of strains that are resistant to multiple antimicrobial agents. multidrug efflux pumps play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. in the present study, we investigated the role of the k. pneumoniae acrrab operon in antimicrobial resistance and virulence by using ... | 2010 | 19858254 |
| the in-vitro antimicrobial activity of some medicinal plants against beta-lactam-resistant bacteria. | in effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria. | 2009 | 19858568 |
| why hypothetical protein kpn00728 of klebsiella pneumoniae should be classified as chain c of succinate dehydrogenase? | twenty percent of genes that encode for hypothetical proteins from klebsiella pneumoniae mgh78578 were identified, leading to kpn00728 and kpn00729 after bioinformatics analysis. both open reading frames showed high sequence homology to succinate dehydrogenase chain c (sdhc) and d (sdhd) from escherichia coli. recently, kpn00729 was assigned as sdhd. kpn00728 thus remains of particular interest as no annotated genes from the complete genome sequence encode for sdhc. we discovered kpn00728 has a ... | 2009 | 19859792 |
| [bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008]. | bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ... | 2009 | 19860320 |
| isolation of cronobacter spp. (formerly enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, pcr and 16s rrna sequencing. | cronobacter spp. (formerly enterobacter sakazakii), are a group of gram-negative pathogens that have been implicated as causative agents of meningitis and necrotizing enterocolitis in infants. the pathogens are linked to infant formula; however, they have also been isolated from a wide range of foods and environmental samples. | 2009 | 19860874 |
| [evolution of enterobacteriaceae resistance to antibiotics in reunion island: emergence of extended-spectrum beta-lactamases]. | the antibiotic resistance of enterobacteriacae knows a worldwide worrying evolution with an increase of the extended spectrum betalactamases (esbl) that spread into the community. few publications describe this problem in the indian ocean area. the aim of this study is first to identify in félix guyon hospital (reunion island) the emergent antibiotics resistance for enterobacteriaceae between 1997/1998 and 2006/2007 periods, at second, to update the prophylactic and therapeutic measures for hand ... | 2010 | 19864085 |
| identification, cloning, and functional characterization of emrd-3, a putative multidrug efflux pump of the major facilitator superfamily from vibrio cholerae o395. | a putative multidrug efflux pump, emrd-3, belonging to the major facilitator superfamily (mfs) of transporters and sharing homology with the bcr/cfla subfamily, was identified in vibrio cholerae o395. we cloned the emrd-3 gene and evaluated its role in antimicrobial efflux in a hypersensitive escherichia coli strain. the efflux activity of this membrane protein resulted in lowering the intracellular concentration of ethidium. the recombinant plasmid carrying emrd-3 conferred enhanced resistance ... | 2009 | 19876617 |
| [septic pylephlebitis associated with enterobacter cloacae septicemia]. | septic pylephlebitis or purulent thrombosis of the portal venous system generally results from a progressive extension of suppurated thrombophlebitis, secondary to an intrabdominal infection. germs most often found are escherichia coli and streptococcus, isolation of enterobacter cloacae is unusual. we report a particular observation of septic pylephlebitis associated with e. cloacae bacteremia, without biliary, digestive or pancreatic lesion on the ct-scan. the antibiotic sensitivity pattern of ... | 2010 | 19879707 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| isolation and characterization of two members of the siderophore-microcin family, microcins m and h47. | in this paper we provide the first biochemical evidence of the existence of a family of structure-related antimicrobial peptides, the siderophore-microcins, in the enterobacteriaceae family. we isolated and characterized two novel siderophore-microcins, mccm and mcch47, previously characterized through genetic studies. mccm and mcch47 were expressed from several escherichia coli strains containing the microcin gene clusters. the spectra of their bactericidal activities were found to be restricte ... | 2010 | 19884380 |
| isolation and characterization of two members of the siderophore-microcin family, microcins m and h47. | in this paper we provide the first biochemical evidence of the existence of a family of structure-related antimicrobial peptides, the siderophore-microcins, in the enterobacteriaceae family. we isolated and characterized two novel siderophore-microcins, mccm and mcch47, previously characterized through genetic studies. mccm and mcch47 were expressed from several escherichia coli strains containing the microcin gene clusters. the spectra of their bactericidal activities were found to be restricte ... | 2010 | 19884380 |
| in vitro activities of antimicrobials against six important species of gram-negative bacteria isolated from raw milk samples in korea. | gram-negative bacteria (gnb) with multidrug resistance pose a serious threat to public health. they are environmental pathogens frequently isolated from raw milk and mastitis in dairy cattle. this study was to examine the in vitro antimicrobial activities against 225 isolates belonging to six important species of gnb from mastitic raw milk samples of dairy herds in the republic of korea: acinetobacter baumannii (n = 17), citrobacter freundii (n = 19), enterobacter cloacae (n = 54), klebsiella pn ... | 2010 | 19895257 |
| novel ambler class a carbapenem-hydrolyzing beta-lactamase from a pseudomonas fluorescens isolate from the seine river, paris, france. | a pseudomonas fluorescens isolate (pf-1) resistant to carbapenems was recovered during an environmental survey performed with water from the seine river (paris). it expressed a novel ambler class a carbapenemase, bic-1, sharing 68 and 59% amino acid identities with beta-lactamases sfc-1 from serratia fonticola and the plasmid-encoded kpc-2, respectively. beta-lactamase bic-1 hydrolyzed penicillins, carbapenems, and cephalosporins except ceftazidime and monobactams. the bla(bic-1) gene was chromo ... | 2010 | 19901091 |
| novel ambler class a carbapenem-hydrolyzing beta-lactamase from a pseudomonas fluorescens isolate from the seine river, paris, france. | a pseudomonas fluorescens isolate (pf-1) resistant to carbapenems was recovered during an environmental survey performed with water from the seine river (paris). it expressed a novel ambler class a carbapenemase, bic-1, sharing 68 and 59% amino acid identities with beta-lactamases sfc-1 from serratia fonticola and the plasmid-encoded kpc-2, respectively. beta-lactamase bic-1 hydrolyzed penicillins, carbapenems, and cephalosporins except ceftazidime and monobactams. the bla(bic-1) gene was chromo ... | 2010 | 19901091 |
| dispersal of carbapenemase blavim-1 gene associated with different tn402 variants, mercury transposons, and conjugative plasmids in enterobacteriaceae and pseudomonas aeruginosa. | the emergence of bla(vim-1) within four different genetic platforms from distinct enterobacteriaceae and pseudomonas aeruginosa isolates in an area with a low prevalence of metallo-beta-lactamase producers is reported. forty-three vim-1-producing isolates (including 19 enterobacter cloacae, 2 escherichia coli, and 2 p. aeruginosa isolates, 18 klebsiella pneumoniae isolate, and 2 klebsiella oxytoca isolate) recovered from 2005 to 2007 and corresponding to 15 pulsed-field gel electrophoresis types ... | 2010 | 19901094 |
| plasmid-mediated carbapenem-hydrolyzing enzyme kpc-2 and arma 16s rrna methylase conferring high-level aminoglycoside resistance in carbapenem-resistant enterobacter cloacae in china. | the emergence and spread of carbapenem-resistant enterobacteriaceae in the world are a major concern. we investigated 5 isolates of enterobacter cloacae that were resistant to all clinically available antimicrobial agents, except polymyxin b. the mics of imipenem and aminoglycosides were >32 and >256 mg/l, respectively. all of the isolates produced 5 extended-spectrum beta-lactamase (esbls) with pis of 5.4 (tem-1), 6.7 (kpc-2), 8.2 (shv-12), 8.4 (ctx-m-14), and arma 16s rrna methylase. bla(kpc-2 ... | 2010 | 19903584 |
| in vitro synergy of levofloxacin plus piperacillin/tazobactam against pseudomonas aeruginosa. | in vitro synergy testing using levofloxacin (lvx) plus piperacillin/tazobactam (tzp) was performed by etest and time-kill assay (tka) for 31 unique fluoroquinolone-resistant pseudomonas aeruginosa isolates. the etest method showed synergy for 9/31 (29%) of isolates, while tka showed synergy with 14/31 (45%) of isolates. when comparing the etest method and tka, concordant results for synergy, antagonism, and indifference were obtained for 24/31 (77%) of the isolates tested. | 2009 | 19911066 |
| detecting related cases of bloodstream infections using time-interval distribution modelling. | an algorithm was designed to highlight related bloodstream infections using data from a nosocomial infection surveillance system to help local public health authorities direct specific measures towards clusters of cases. the approach was based on a two-step procedure. the first was a test to identify pathogens with an abnormal number of close cases. the second modelled, for the identified pathogens, the distribution of time intervals between successive cases as a mixture of two theoretical distr ... | 2010 | 19914738 |
| bacteria colonizing root nodules of wild legumes exhibit virulence-associated properties of mammalian pathogens. | bacteria not proficient in nitrogen fixing symbiosis were proven able to invade root nodules of three wild legumes of the genus hedysarum in algeria and to be multiplying in these in place of the natural rhizobium symbionts. the involved species featured taxa known as human pathogens including: enterobacter cloacae, enterobacter kobei, escherichia vulneris, pantoea agglomerans and leclercia adecarboxylata. a direct screening of the phenotypic determinants of virulence using human cultured cells ... | 2010 | 19916054 |
| novel insights about class 2 integrons from experimental and genomic epidemiology. | in order to contribute to the knowledge of the architecture and epidemiology of class 2 integrons, we performed a class 2 integron molecular survey in which we analyzed 726 isolates in two bacterial populations from environmental and nonepidemiologically related clinical samples, respectively, collected from 1982 to 2007. we recovered the inti2 gene from 130 of 726 isolates, most of which were clinical isolates, and only 1 (a psychrophilic pseudomonas sp.) was from a water sample. unlike the wid ... | 2010 | 19917745 |
| antineoplastic agents. 556. isolation and structure of coprinastatin 1 from coprinus cinereus. | cancer cell line bioassay-guided separation of an ethyl acetate extract prepared from a plant-associated fungus, coprinus cinereus, led to the isolation of three new sesquiterpenes, coprinastatin 1 (1), coprinol (2), and the epimer (4a), of the known sesquiterpene triol (4b). the previously described sesquiterpene 3 and oxazolinone 5 were also isolated. the structure and relative configuration of coprinastatin 1 (1) were determined by hrms and by 1d- and 2d-nmr spectroscopic analyses. the struct ... | 2010 | 19919060 |
| structural basis of the inhibition of class a beta-lactamases and penicillin-binding proteins by 6-beta-iodopenicillanate. | 6-beta-halogenopenicillanates are powerful, irreversible inhibitors of various beta-lactamases and penicillin-binding proteins. upon acylation of these enzymes, the inhibitors are thought to undergo a structural rearrangement associated with the departure of the iodide and formation of a dihydrothiazine ring, but, to date, no structural evidence has proven this. 6-beta-iodopenicillanic acid (bip) is shown here to be an active antibiotic against various bacterial strains and an effective inhibito ... | 2009 | 19919161 |
| characteristics of doripenem: a new broad-spectrum antibiotic. | doripenem (s-4661) is a new parenteral antibiotic from the carbapenem class; similarly to imipenem and meropenem, it has a broad-spectrum activity against gram-positive, gram-negative, and anaerobic bacteria. it is active against multiresistant gram-negative bacilli such as extended-spectrum beta-lactamase-producing (esbl) gram-negative enterobacteriaceae and nonfermentative gram-negative bacilli including some strains of pseudomonas aeruginosa that are resistant to other carbapenems. doripenem' ... | 2009 | 19920933 |
| novel integron gene cassette arrays identified in a global collection of multi-drug resistant non-typhoidal salmonella enterica. | investigation of integron carriage in a global collection of multi-drug resistant salmonella enterica identified 3 unique class 1 integron gene cassette arrays not previously reported in this species. the present study used pcr and dna sequence analysis to characterize the structure of these gene cassette arrays. a approximately 4.0 kb integron containing the gene cassette array arr2/cmla5/bla (oxa10) /aada1 was found in isolates belonging to serovars isangi and typhimurium from south africa. a ... | 2010 | 19921331 |
| robustness of the bacterial community in the cabbage white butterfly larval midgut. | microbial communities typically vary in composition and structure over space and time. little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. in this study, we use 16s ribosomal rna gene sequences to describe variation among bacterial communities in the midguts of cabbage white butterfly (pieris rapae) larvae and examine the influence of community ... | 2010 | 19924467 |
| robustness of the bacterial community in the cabbage white butterfly larval midgut. | microbial communities typically vary in composition and structure over space and time. little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. in this study, we use 16s ribosomal rna gene sequences to describe variation among bacterial communities in the midguts of cabbage white butterfly (pieris rapae) larvae and examine the influence of community ... | 2010 | 19924467 |
| inhibition of the class c beta-lactamase from acinetobacter spp.: insights into effective inhibitor design. | the need to develop beta-lactamase inhibitors against class c cephalosporinases of gram-negative pathogens represents an urgent clinical priority. to respond to this challenge, five boronic acid derivatives, including a new cefoperazone analogue, were synthesized and tested against the class c cephalosporinase of acinetobacter baumannii [acinetobacter-derived cephalosporinase (adc)]. the commercially available carbapenem antibiotics were also assayed. in the boronic acid series, a chiral cephalo ... | 2010 | 19925018 |
| characterization of expression, activity and role in antibacterial immunity of anopheles gambiae lysozyme c-1. | there are eight lysozyme genes in the anopheles gambiae genome. transcripts of one of these genes, lysc-1, increased in anopheles gambiae cell line 4a3b by 24 h after exposure to heat-killed micrococcus luteus. lysozyme activity was also identified in conditioned media from the cell line from which the protein was purified to homogeneity using ion exchange and gel filtration. mass spectrometric analysis of the purified protein showed 100% identity to lysozyme c-1. purified lysozyme c-1 was teste ... | 2010 | 19932188 |
| substituted aryl malonamates as new serine beta-lactamase substrates: structure-activity studies. | a series of substituted aryl malonamates have been prepared. these compounds are analogues of aryl phenaceturates where the amido side chain has been replaced by a retro-amide. like the phenaceturates, these compounds are substrates of typical class a and class c beta-lactamases, particularly of the latter, and of soluble dd-peptidases. the effect of substituents alpha to the ester carbonyl group on turnover by these enzymes is similar to that in the phenaceturates. on the other hand, n-alkylati ... | 2010 | 19932622 |
| substituted aryl malonamates as new serine beta-lactamase substrates: structure-activity studies. | a series of substituted aryl malonamates have been prepared. these compounds are analogues of aryl phenaceturates where the amido side chain has been replaced by a retro-amide. like the phenaceturates, these compounds are substrates of typical class a and class c beta-lactamases, particularly of the latter, and of soluble dd-peptidases. the effect of substituents alpha to the ester carbonyl group on turnover by these enzymes is similar to that in the phenaceturates. on the other hand, n-alkylati ... | 2010 | 19932622 |
| extended spectrum beta-lactamases among gram-negative bacterial isolates from clinical specimens in three major hospitals in northern jordan. | background and objectives. extended spectrum beta-lactamase (esbl) production is increasing all over the world, and organisms other than e. coli and k. pneumoniae are acquiring this character. esbl production is detectable by automation, e-test, double disk diffusion (ddd), and pcr. this study aimed to determine the prevalence of esbl production among clinical isolates of gram-negative rods, and to evaluate the effectiveness of augmentation of clavunate with cefotaxime, ceftazoxime, aztreonam, c ... | 2009 | 19936109 |
| enterobacteriaceae and pseudomonadaceae on the dorsum of the human tongue. | the aim of this study was to correlate the presence of enterobacteriaceae, pseudomonadaceae, moraxellaceae and xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use. | 2009 | 19936511 |
| pyogenic vertebral osteomyelitis: identification of microorganism and laboratory markers used to predict clinical outcome. | the aim of this study is to determine the predictive values of laboratory indicators of pyogenic vertebral osteomyelitis (pvo) and a potential cure if the microorganism cannot be identified. forty-five consecutive patients with pvo were enrolled. antibiotic therapy with or without surgery was performed according to microorganism. in the negative-culture (nc) group, cefazolin was administered in cases of hematogenous pvo, and vancomycin was administered in cases of postoperative or procedure-rela ... | 2010 | 19937064 |
| pyogenic vertebral osteomyelitis: identification of microorganism and laboratory markers used to predict clinical outcome. | the aim of this study is to determine the predictive values of laboratory indicators of pyogenic vertebral osteomyelitis (pvo) and a potential cure if the microorganism cannot be identified. forty-five consecutive patients with pvo were enrolled. antibiotic therapy with or without surgery was performed according to microorganism. in the negative-culture (nc) group, cefazolin was administered in cases of hematogenous pvo, and vancomycin was administered in cases of postoperative or procedure-rela ... | 2010 | 19937064 |
| direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. | with long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (maldi-tof) mass spectrometry was demonstrated to accurately identify bacteria routinely isolated in a clinical biology laboratory. in order to speed up the identification proce ... | 2009 | 19946369 |
| a one-step, real-time pcr assay for rapid detection of rhinovirus. | one-step, real-time pcr assays for rhinovirus have been developed for a limited number of pcr amplification platforms and chemistries, and some exhibit cross-reactivity with genetically similar enteroviruses. we developed a one-step, real-time pcr assay for rhinovirus by using a sequence detection system (applied biosystems; foster city, ca). the primers were designed to amplify a 120-base target in the noncoding region of picornavirus rna, and a taqman (applied biosystems) degenerate probe was ... | 2010 | 19948820 |
| prevalence of extended-spectrum beta-lactamases in enterobacter cloacae in taiwan and comparison of 3 phenotypic confirmatory methods for detecting extended-spectrum beta-lactamase production. | the prevalence of extended-spectrum beta-lactamases (esbls) in enterobacter cloacae remains unclear in taiwan. this study was conducted to investigate the prevalence of esbl-producing e. cloacae in taiwan. | 2009 | 19949754 |
| overexpression of hns in the plant growth-promoting bacterium enterobacter cloacae uw5 increases root colonization. | plant growth-promoting rhizobacteria (pgpr) introduced into soil often do not compete effectively with indigenous micro-organisms for plant colonization. the aim of this study was to identify novel genes that are important for root colonization by the pgpr enterobacter cloacae uw5. | 2010 | 19951377 |
| multilocus sequence analysis for typing leptospira interrogans and leptospira kirschneri. | fifty-three strains belonging to the pathogenic species leptospira interrogans and leptospira kirschneri were analyzed by multilocus sequence analysis. the species formed two distinct branches. in the l. interrogans branch, the phylogenetic tree clustered the strains into three subgroups. genogroups and serogroups were superimposed but not strictly. | 2010 | 19955271 |
| multilocus sequence analysis for typing leptospira interrogans and leptospira kirschneri. | fifty-three strains belonging to the pathogenic species leptospira interrogans and leptospira kirschneri were analyzed by multilocus sequence analysis. the species formed two distinct branches. in the l. interrogans branch, the phylogenetic tree clustered the strains into three subgroups. genogroups and serogroups were superimposed but not strictly. | 2010 | 19955271 |
| rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry is a rapid, accurate method for identifying bacteria and fungi recovered on agar culture media. we report herein a method for the direct identification of bacteria in positive blood culture broths by maldi-tof mass spectrometry. a total of 212 positive cultures were examined, representing 32 genera and 60 species or groups. the identification of bacterial isolates by maldi-tof mass spectrometry was compared ... | 2010 | 19955282 |
| rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry is a rapid, accurate method for identifying bacteria and fungi recovered on agar culture media. we report herein a method for the direct identification of bacteria in positive blood culture broths by maldi-tof mass spectrometry. a total of 212 positive cultures were examined, representing 32 genera and 60 species or groups. the identification of bacterial isolates by maldi-tof mass spectrometry was compared ... | 2010 | 19955282 |
| [analysis of antibiotics treatment in 86 cases of liver transplant recipients]. | to analyze the characteristic of bacterial infections, and the relationship between antibiotics treatment and bacterial infections after liver transplantation, and to prevent antibiotic-resistant bacterial infections. | 2009 | 19958647 |
| a case of asymptomatic fungal and bacterial colonization of an intragastric balloon. | intragastric balloon therapy, as a part of a multidisciplinary weight management program, is an effective short-term intervention for weight loss. although the insertion procedure is easy and generally well tolerated by patients, a few complications can occur. we report here a heavy smoker with intragastric balloon insertion complicated by colonization with opportunistic organisms. the 27-year-old female, body mass index 35.5 kg/m(2), had a bioenterics intragastric balloon inserted under conscio ... | 2009 | 19960576 |
| retained hawthorn fragment in a child's foot complicated by infection: diagnosis and excision aided by localization with ultrasound. | puncture wounds in children are very common and often result in foreign body retention. organic materials in the form of plant thorns present problems in identification and localization because they are not visualized with plain radiographs. a case of a 10-year-old girl with a small piece of retained hawthorn is presented. correct diagnosis and treatment were delayed because of misinterpretation of magnetic resonance image studies. ultrasound ultimately located the foreign body, and assisted in ... | 2010 | 19962326 |
| molybdate reduction by pseudomonas sp. strain dry2. | to isolate and characterize a potent molybdenum-reducing bacterium. | 2010 | 19968732 |
| international spread and persistence of tem-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an inca/c2 plasmid containing tn1696::tn1 and is5075-tn21. | tem-24 remains one of the most widespread tem-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae. to analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative tem-24 producers from belgium, france, portugal, and spain (13 enterobacter aerogenes isolates, 6 escherichia coli isolates, 6 klebsiella pneumoniae isolates, 2 proteus mirabilis isolates, and 1 klebsiella oxytoca isolate, from 1998 to 2004). ... | 2010 | 19995930 |
| international spread and persistence of tem-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an inca/c2 plasmid containing tn1696::tn1 and is5075-tn21. | tem-24 remains one of the most widespread tem-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae. to analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative tem-24 producers from belgium, france, portugal, and spain (13 enterobacter aerogenes isolates, 6 escherichia coli isolates, 6 klebsiella pneumoniae isolates, 2 proteus mirabilis isolates, and 1 klebsiella oxytoca isolate, from 1998 to 2004). ... | 2010 | 19995930 |
| colistin therapy for microbiologically documented multidrug-resistant gram-negative bacterial infections: a retrospective cohort study of 258 patients. | it is unclear whether the effectiveness of polymyxins depends on the site of infection, the responsible pathogen, dosage, and monotherapy vs. combination therapy. we investigated colistin therapy in a large, retrospective, single-centre, cohort study. primary analysis outcomes were infection outcome, survival and nephrotoxicity. over a 7-year period (october 2000 to october 2007), 258 patients received intravenous (i.v.) colistin for at least 72h for microbiologically documented multidrug-resist ... | 2010 | 20006471 |
| wound botulism complicating internal fixation of a complex radial fracture. | botulism developed in a patient following surgical repair of an open radial fracture. symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew clostridium argentinense. this case highlights the management of botulism associated with orthopedic hardware. | 2010 | 20007390 |
| wound botulism complicating internal fixation of a complex radial fracture. | botulism developed in a patient following surgical repair of an open radial fracture. symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew clostridium argentinense. this case highlights the management of botulism associated with orthopedic hardware. | 2010 | 20007390 |