| [isolation and biodiversity of copper-resistant bacteria from rhizosphere soil of elsholtzia splendens]. | isolation and characterization of rhizosphere copper-resistant bacteria from a copper accumulator plant elsholtzia splendens were investigated. | 2009 | 20069883 |
| mechanism of binding of the inhibitor (e)-3-(furan-2-yl)-n-hydroxyacrylamide to a histone deacetylase-like amidohydrolase. | histone deacetylases have proven to be attractive novel targets for the treatment of cancer. the first inhibitor of histone deacetylases was approved for the treatment of cutaneous t-cell lymphoma in 2006. the identification of new lead structures with improved effectiveness and fewer side effects is necessary. this report investigates the mechanism of inhibition of a histone deacetylase-like amidohydrolase by stopped-flow and equilibrium titration techniques. the interaction between the inhibit ... | 2010 | 20082520 |
| directed evolution of copper nitrite reductase to a chromogenic reductant. | directed evolution methods were developed for cu-containing nitrite reductase (nir) from alcaligenes faecalis s-6. the pcr cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. the high-throughput screen includes colony lift onto a nylon membrane and subsequent lysis of nir-expressing colonies in the presence of cu(2+) ions for copper incorporation into intracellularly expressed nir. addition ... | 2010 | 20083495 |
| polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent. | biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ... | 2010 | 20093025 |
| [microbial load of reprocessable trocars after gynecological videolaparoscopy]. | to identify the microbial charge present in reusable trocars used in gynecological laparoscopies. | 2009 | 20101372 |
| biodegradation of the major color containing compounds in distillery wastewater by an aerobic bacterial culture and characterization of their metabolites. | this study deals the biodegradation of the major color containing compounds extracted from distillery wastewater (dww) by an aerobic bacterial consortium comprising bacillus licheniformis (dq79010), bacillus sp. (dq779011) and alcaligenes sp. (dq779012) and characterization of metabolic products. the degradation of color containing compounds by bacteria was studied by using the different carbon and nitrogen sources at different environmental conditions. results revealed that the bacterial consor ... | 2010 | 20146090 |
| a combined metabolic/polymerization kinetic model on the microbial production of poly(3-hydroxybutyrate). | in the present work, an integrated dynamic metabolic/polymerization kinetic model is developed for the prediction of the intracellular accumulation profile and the molecular weight distribution of poly(3-hydroxybutyrate) (p(3hb) or phb) produced in microbial cultures. the model integrates two different length/time scales by combining a polymerization kinetic model with a metabolic one. the bridging point between the two models is the concentration of the monomer unit (i.e. 3-hydroxybutyryl-coa) ... | 2010 | 20153452 |
| construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol. | pseudomonas sp. strain nyz402, a native soil organism that grows on para-nitrophenol (pnp), was genetically engineered for the simultaneous degradation of methyl parathion (mp) and ortho-nitrophenol (onp) by integrating mph (methyl parathion hydrolase gene) from pseudomonas sp. strain wbc-3 and onpab (onp 2-monooxygenase and onp o-benzoquinone reductase genes) from alcaligenes sp. strain nyz215 into the genome of strain nyz402. methyl parathion hydrolase (mph), onp 2-monooxygenase (onpa) and o-b ... | 2010 | 20157808 |
| characterization of two diesel fuel degrading microbial consortia enriched from a non acclimated, complex source of microorganisms. | the bioremediation of soils impacted by diesel fuels is very often limited by the lack of indigenous microflora with the required broad substrate specificity. in such cases, the soil inoculation with cultures with the desired catabolic capabilities (bioaugmentation) is an essential option. the use of consortia of microorganisms obtained from rich sources of microbes (e.g., sludges, composts, manure) via enrichment (i.e., serial growth transfers) on the polluting hydrocarbons would provide biorem ... | 2010 | 20158909 |
| detection of coumaphos in honey using a screening method based on an electrochemical acetylcholinesterase bioassay. | an analytical protocol based on an electrochemical assay for the detection of acetylcholinesterase (ache) inhibitors has been optimised for the detection of coumaphos in honey. coumaphos is a phosphotionate insecticide requiring transformation in the corresponding oxo-form to act as an effective ache inhibitor. the inhibition assay was based on the electrochemical detection of the product of ache, choline, via a choline oxidase biosensors obtained using prussian-blue modified screen printed elec ... | 2010 | 20188890 |
| functional characteristics and diversity of a novel lignocelluloses degrading composite microbial system with high xylanase activity. | to obtain an efficient natural lignocellulolytic complex enzyme, we screened an efficient lignocellulose degrading composite microbial system (xdc-2) from composted agricultural and animal wastes amended soil following a long-term directed acclimation. the xdc-2 could not only degrade natural lignocelluloses, but could also secret extracellular xylanase efficiently in liquid culture under static conditions at room temperature. the xdc-2 degraded rice straw by 60.3% after fermentation for 15 days ... | 2010 | 20208427 |
| improved a. faecalis penicillin amidase mutant retains the thermodynamic and ph stability of the wild type enzyme. | penicillin amidase from alacaligenes faecalis is an attractive biocatalyst for hydrolysis of penicillin g for production of 6-aminopenicillanic acid, which is used in the synthesis of semi-synthetic beta-lactam antibiotics. recently a mutant of this enzyme with extended c-terminus of the a-chain comprising parts of the connecting linker peptide was constructed. its turnover number for the hydrolysis of penicillin g was 140 s(-1), about twice of the value for the wild-type enzyme (80 s(-1)). at t ... | 2010 | 20224955 |
| evaluation of otoscope cone disinfection techniques and contamination level in small animal private practice. | the objective of this study was to evaluate the level of bacterial contamination of otoscope cones in veterinary private practice, and to determine the most effective method of disinfection. fifty small animal practices participated in this study, which included a detailed survey regarding otoscope cleaning, storage and usage and quantitative culture of the cleaned and stored otoscope cones. using sterile technique, two cones from each of the 50 hospitals were swabbed and submitted for quantitat ... | 2010 | 20230588 |
| bacteremia and acute meningitis due to alcaligenes faecalis; a case report. | | 1947 | 20267290 |
| recent advances in the treatment of cardiovascular disease. | | 1947 | 20267438 |
| occurrence of bact. alcaligenes sp. in duck eggs. | | 1946 | 20291458 |
| occurrence of bact. alcaligenes sp. in duck eggs. | | 1947 | 20295907 |
| characterization of phenol degradation by high-efficiency binary mixed culture. | two new high phenol-degrading strains, micrococcus sp. and alcaligenes faecalis jh 1013, were isolated. the two isolates could grow aerobically in mineral salts medium containing phenol as a sole carbon source at concentration of 3,000 mg l(-1). it was found that the binary mixed culture of the two isolates possessed good potential for phenol removal. | 2010 | 20300870 |
| diversity of microbes and potential exoelectrogenic bacteria on anode surface in microbial fuel cells. | single-chamber microbial fuel cells (mfcs), inoculated with anaerobic sludge and continuously run with two kinds of organic wastewater influents, were systemically investigated. the diversity of microbes, determined by 16s rdna analysis, was analyzed on three anodes under different conditions. one anode was in a closed circuit in synthetic wastewater containing glucose. the other two anodes, in open or closed circuits, were fed effluent from an anaerobic reactor treating starch wastewater. the c ... | 2010 | 20339216 |
| indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis. | the indigenous bacteria create natural cohabitation niches together with mucosal abs in the gastrointestinal (gi) tract. here we report that opportunistic bacteria, largely alcaligenes species, specifically inhabit host peyer's patches (pps) and isolated lymphoid follicles, with the associated preferential induction of antigen-specific mucosal iga abs in the gi tract. alcaligenes were identified as the dominant bacteria on the interior of pps from naïve, specific-pathogen-free but not from germ- ... | 2010 | 20360558 |
| microbial degradation of 17beta -estradiol and 17alpha -ethinylestradiol followed by a validated hplc-dad method. | this work aimed at studying the biodegradation of two estrogens, 17alpha -estradiol (e2) and 17beta -ethinylestradiol (ee2), and their potential metabolism to estrone (e1) by microbial consortia. the biodegradation studies were followed by high performance liquid chromatography-diode array detector (hplc-dad) using a specifically developed and validated method. biodegradation studies of the estrogens (e2 and ee2) were carried out with activated sludge (consortium a, ca) obtained from a wastewate ... | 2010 | 20390962 |
| electrical contacting of an assembly of pseudoazurin and nitrite reductase using dna-directed immobilization. | a method for the electrical contacting of redox enzymes that obtain oxidizing or reducing equivalents from small electron-transfer proteins is demonstrated. the electrochemical contacting of redox enzymes through their immobilization onto electrode supports offers great potential for technological applications and for fundamental studies, but finding appropriate methods to immobilize the enzymes in an orientation allowing rapid electron transfer with the electrode has proven difficult. the coppe ... | 2010 | 20397667 |
| biocatalytic synthesis of (r)-(-)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of alcaligenes faecalis ecu0401. | the nitrilase from alcaligenes faecalis ecu0401 belongs to the category of arylacetonitrilase, which could hydrolyze 2-chloromandelonitrile, 3,4-dimethoxyphenylacetonitrile, mandelonitrile, and phenylacetonitrile into the corresponding arylacetic acids. to overcome the permeability barrier and prepare whole cell biocatalysts with high activities, permeabilization of alcaligenes faecalis ecu0401 in relation to nitrilase activity was optimized by using cetyltrimethylammonium bromide (ctab) as perm ... | 2010 | 20411403 |
| the small subunit arob of arsenite oxidase: lessons on the [2fe-2s] rieske protein superfamily. | here, we describe the characterization of the [2fe-2s] clusters of arsenite oxidases from rhizobium sp. nt-26 and ralstonia sp. 22. both reduced rieske proteins feature epr signals similar to their homologs from rieske-cyt b complexes, with g values at 2.027, 1.88, and 1.77. redox titrations in a range of ph values showed that both [2fe-2s] centers have constant e(m) values up to ph 8 at approximately +210 mv. above this ph value, the e(m) values of both centers are ph-dependent, similar to what ... | 2010 | 20421651 |
| a new shuttle vector for gene expression in biopolymer-producing ralstonia eutropha. | ralstonia eutropha (formerly alcaligenes eutrophus) is a fascinating microorganism with a great scientific importance and an immense commercial potential. a new genetic transformation system for the organism would greatly facilitate the biological study and molecular engineering of this organism. we report here a versatile gene expression method for the genetic engineering of r. eutropha. this method, based on a simplified electroporation protocol, uses a recombinant plasmid, pbs29-p2, containin ... | 2010 | 20447426 |
| [investigation of biotechnological strains of microorganisms for their hygienic standardization]. | by analyzing the findings and databases on the hygienic standardization of industrial microorganisms, the authors have improved a research program for industrial strains on substantiating their safe exposure levels in workplace and ambient airs. a shortened program is proposed to investigate gram-positive bacteria and nocardioformic actinomycetes (lactobacillus, micrococcus, propionibacterim, brevibacterim, rhodococcus), which have no negative effect. it is necessary to study gram-negative bacte ... | 2010 | 20491276 |
| analysis of biological demulsification process of water-in-oil emulsion by alcaligenes sp. s-xj-1. | a demulsifying strain (s-xj-1) was isolated from petroleum-polluted soil and identified as alcaligenes sp. it showed emulsion breaking ratio of 81.3% for w/o emulsion within 24h when the cell concentration was 500mg/l. evolution of water droplets during the biological demulsification process was investigated using a turbiscan stability analyzer and microphotography. further investigation focused on cell surface hydrophobicity and oil-water interfacial properties. the biological demulsification p ... | 2010 | 20576429 |
| validation of a lanthanide tag for the analysis of protein dynamics by paramagnetic nmr spectroscopy. | paramagnetic lanthanide tags potentially can enhance the effects of microsecond to millisecond dynamics in proteins on nmr signals and provide structural information on lowly populated states encoded in the pseudocontact shifts. we have investigated the microsecond to millisecond mobility of a two-point attached lanthanide tag, clanp-5, using paramagnetic (1)h cpmg relaxation dispersion methods. clanp-5 loaded with lu(3+), yb(3+), or tm(3+) was attached to three sites on the surface of two prote ... | 2010 | 20586489 |
| enhanced welan gum production using a two-stage agitation speed control strategy in alcaligenes sp. cgmcc2428. | batch fermentative production of welan gum by alcaligenes sp. cgmcc2428 was investigated under various oxygen supply conditions using regulating agitation speed. based on a three kinetic parameters analysis that includes specific cell growth rate (μ), specific glucose consumption rate (q (s)), and specific welan formation rate (q (p)), a two-stage agitation speed control strategy was proposed to achieve high concentration, high yield, and high viscosity of welan. during the first 22 h, the agita ... | 2011 | 20640447 |
| enantioselective biocatalytic hydrolysis of (r,s)-mandelonitrile for production of (r)-(-)-mandelic acid by a newly isolated mutant strain. | (r)-(-)-mandelic acid (r-ma) is an important intermediate with broad uses. recently, r-ma production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. in this work, a new bacterium wt10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of r-ma by enantioselective biocatalytic hydrolysis of (r,s)-mandelonitrile, was isolated and identified as a strain of alcaligenes faecalis. in order to impr ... | 2011 | 20640871 |
| characterization of soluble microbial products (smp) under stressful conditions. | soluble microbial products (smp) in the wastewater treatment process not only cause fouling to the membrane, but also generate disinfection by-products (dbp) in the effluent, thus get increasing attention. in this study, smp produced by activated sludge and isolates under different stressful conditions, i.e. starvation, salinity, heavy metals, low ph and high temperature, were characterized to investigate the effects of these conditions on the amount of smp and their compositions. the analysis r ... | 2010 | 20655085 |
| capd peritonitis caused by alcaligenes xylosoxidans in a diabetic cirrhosis patient. | despite the advancement of disconnecting system, peritonitis remains one of the most common causes in patients treated with continuous ambulatory peritoneal dialysis (capd). we report a cirrhosis patient undergoing capd who suffered from an episode of peritonitis caused by a rare pathogen, alcaligenes xylosoxidans (ax). this organism rarely induces capd peritonitis and only nine cases were reported in the literature. this is the first case of ax-related capd peritonitis in cirrhosis patient. inf ... | 2010 | 20662708 |
| linalool dehydratase-isomerase, a bifunctional enzyme in the anaerobic degradation of monoterpenes. | castellaniella (ex alcaligenes) defragrans strain 65phen mineralizes monoterpenes in the absence of oxygen. soluble cell extracts anaerobically catalyzed the isomerization of geraniol to linalool and the dehydration of linalool to myrcene. the linalool dehydratase was present in cells grown on monoterpenes, but not if grown on acetate. we purified the novel enzyme ∼1800-fold to complete homogeneity. the native enzyme had a molecular mass of 160 kda. denaturing gel electrophoresis revealed one si ... | 2010 | 20663876 |
| [microbial contamination of nipradiol with and without benzalkonium chloride]. | to compare microbial contamination of nipradiol both with and without benzalkonium chloride (bac). | 2010 | 20681256 |
| [isolation, identification and characterization of a high-efficient heterotrophic nitrifier]. | in order to improve the rate of the heterotrophic nitrification, we screened and identified a high-efficient heterotrophic nitrifier, as well as studied its nitrification characteristics and nitrification conditions. | 2010 | 20687347 |
| optimization of biodemulsifier production from alcaligenes sp. s-xj-1 and its application in breaking crude oil emulsion. | a biodemulsifier-producing strain of alcaligenes sp. s-xj-1, isolated from petroleum-contaminated soil of the karamay oilfield, exhibited excellent demulsifying ability. the application of this biodemulsifier significantly improved the quality of separated water compared with the chemical demulsifier, polyether, which clearly indicates that it has potential applications in the crude oil extraction industry. to optimize its biosynthesis, the impacts of carbon sources, nitrogen sources and ph were ... | 2010 | 20702035 |
| long-term hg pollution-induced structural shifts of bacterial community in the terrestrial isopod (porcellio scaber) gut. | in previous studies we detected lower species richness and lower hg sensitivity of the bacteria present in egested guts of porcellio scaber (crustacea, isopoda) from chronically hg polluted than from unpolluted environment. basis for such results were further investigated by sequencing of 16s rrna genes of mercury-resistant (hgr) isolates and clone libraries. we observed up to 385 times higher numbers of hgr bacteria in guts of animals from polluted than from unpolluted environment. the majority ... | 2010 | 20724045 |
| biodegradation of phenanthrene by alcaligenes sp. strain pph: partial purification and characterization of 1-hydroxy-2-naphthoic acid hydroxylase. | alcaligenes sp. strain pph degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-h2na), 1,2-dihydroxynaphthalene (1,2-dhn), salicylic acid and catechol. enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase and salicylate hydroxylase. 1-hydroxy-2-naphthoic acid hydroxylase was partially purified (yield 48%, fold 81) and found to be a homodimer with a subunit molecular weight of ∼34 kda ... | 2010 | 20727010 |
| effect of bimetallic and polymer-coated fe nanoparticles on biological denitrification. | bimetallic nanoparticles (nano fe-ni, nano fe-cu) and coated iron nanoparticles (chitosan-fe(0), sodium oleate-fe(0)) were utilized to support autotrophic denitrification. in comparison to nanoscale zero-valent iron (nzvi) particles, ni-containing nanoparticles resulted in faster nitrate removal, but generated 17% more ammonium. the nano fe-cu integrated system, required two days less than the unmodified nzvi integrated system to remove all the nitrate and decrease ammonium by 13%, but a large a ... | 2010 | 20727742 |
| component identification of electron transport chains in curdlan-producing agrobacterium sp. atcc 31749 and its genome-specific prediction using comparative genome and phylogenetic trees analysis. | agrobacterium sp. atcc 31749 (formerly named alcaligenes faecalis var. myxogenes) is a non-pathogenic aerobic soil bacterium used in large scale biotechnological production of curdlan. however, little is known about its genomic information. dna partial sequence of electron transport chains (etcs) protein genes were obtained in order to understand the components of etc and genomic-specificity in agrobacterium sp. atcc 31749. degenerate primers were designed according to etc conserved sequences in ... | 2010 | 20730594 |
| bacillaemia due to infection with b. faecalis alcaligenes. | | 1916 | 20768418 |
| modification of cell surface properties of pseudomonas alcaligenes s22 during hydrocarbon biodegradation. | biodegradation of water insoluble hydrocarbons can be significantly increased by the addition of natural surfactants one. very promising option is the use of saponins. the obtained results indicated that in this system, after 21 days, 92% biodegradation of diesel oil could be achieved using pseudomonas alcaligenes. no positive effect on the biodegradation process was observed using synthetic surfactant triton x-100. the kind of carbon source influences the cell surface properties of microorganis ... | 2011 | 20820883 |
| predacious bacteria, bdellovibrio with potential for biocontrol. | bacteria of the genus of bdellovibrio are highly motile gram-negative predators of other gram-negative bacteria causing lysis of their prey. here we report results of studies on the interactions of bdellovibrio with species of alcaligenes, campylobacter, erwinia, escherichia, helicobacter, pseudomonas, legionella, and shigella in agar lower, liquid media and cells attached to a surface. helicobacter pylori was studied employing both actively growing and viable but nonculturable (vbnc) cells. the ... | 2010 | 20850380 |
| selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases. | a microbial community was selected for growth on dried and naoh-treated switchgrass. during a 14-day liquid cultivation, a 70% loss in dry weight was observed during the first 4 days and after 14 days, the hemicellulose and cellulose in the system were degraded by 73.5% and 67.3%, respectively. the carboxymethyl cellulase (cmcase) and xylanase levels reached 0.21 and 3.75 iu, respectively. the optimal ph for cmcase and xylanase activities was 5 and 6, respectively. the optimal reaction temperatu ... | 2010 | 20933405 |
| the crystal structure of cobalt-substituted pseudoazurin from alcaligenes faecalis. | the cu(ii) center at the active site of the blue copper protein pseudoazurin from alcaligenes faecalis has been substituted by co(ii) via denaturing of the protein, chelation and removal of copper by edta and refolding of the apo-protein, followed by addition of an aqueous solution of cocl(2). sitting drop vapour diffusion experiments produced green hexagonal crystals, which belong to space group p6(5), with unit cell dimensions a = b = 50.03, c = 98.80 å. diffraction data, collected at 291 k on ... | 2011 | 20945335 |
| cytochromes: reactivity of the "dark side" of the heme. | ligand binding to the heme distal side is a paradigm of heme-protein biochemistry, the proximal axial ligand being in most cases a his residue. no binds to the ferrous heme-fe-atom giving rise to hexa-coordinated adducts (as in myoglobin and hemoglobin) with his and no as proximal and distal axial ligands, respectively, or to penta-coordinated adducts (as in soluble guanylate cyclase) with no as the axial distal ligand. recently, the ferrous derivative of alcaligenes xylosoxidans cytochrome c' ( ... | 2010 | 20952122 |
| potential pathogenic bacteria in metalworking fluids and aerosols from a machining facility. | the metalworking and machining industry utilizes recirculating metalworking fluids for integral aspects of the fabrication process. despite the use of biocides, these fluids sustain substantial biological growth. subsequently, the high-shear forces incurred during metalworking processing aerosolize bacterial cells and may cause dermatologic and respiratory effects in exposed workers. we quantified and identified the bacterial load for metalworking fluid and aerosol samples of a machining facilit ... | 2010 | 20955193 |
| cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from alcaligenes sp. ecu0401 and its potential for (r)-(-)-mandelic acid production. | a nitrilase gene from alcaligenes sp. ecu0401 was cloned and overexpressed in escherichia coli bl21 (de3) in a soluble form. the encoded protein with a his₆-tag was purified to nearly homogeneity as revealed by sds-page with a molecular weight of approximately 38.5 kda, and the holoenzyme was estimated to be composed of 10 subunits of identical size by size exclusion chromatography. the v(max) and k(m) parameters were determined to be 27.9 μmol min⁻¹ mg⁻¹ protein and 21.8 mm, respectively, with ... | 2010 | 20960011 |
| effect of penicillin on growth of alcaligenes fecalis. | | 1946 | 21017956 |
| [not available]. | | 1946 | 21027747 |
| a high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry. | nitrile-hydrolyzing enzymes (nitrilase or nitrile hydratase/amidase) have been widely used in the pharmaceutical industry for the production of carboxylic acids and their derivatives, and it is important to build a method for screening for nitrile-hydrolyzing enzymes. in this paper, a simple, rapid, and high-throughput screening method based on the ferric hydroxamate spectrophotometry has been proposed. to validate the accuracy of this screening strategy, the nitrilases from rhodococcus erythrop ... | 2010 | 21038095 |
| kinetic modeling and half life study on bioremediation of crude oil dispersed by corexit 9500. | hydrocarbon pollution in marine ecosystems occurs mainly by accidental oil spills, deliberate discharge of ballast waters from oil tankers and bilge waste discharges; causing site pollution and serious adverse effects on aquatic environments as well as human health. a large number of petroleum hydrocarbons are biodegradable, thus bioremediation has become an important method for the restoration of oil polluted areas. in this research, a series of natural attenuation, crude oil (co) and dispersed ... | 2010 | 21041026 |
| [production of biodemulsifier by alcaligenes sp. s-xj-1 using waste diesel oil]. | biodemulsifier is a new type of demulsifiers for breaking oil-water emulsion. one demulsifier-producing strain, alcaligenes sp. s-xj-1 could grow on waste diesel oil (wdo), dry weight of the strain was up to 2.0 g/l after being cultivated for 7 d, 10 g/l s-xj-1 cell suspension reduced surface tension of water from 72.0 mn/m to 29.7 mn/m. biodemulsifier produced by s-xj-1 was 0.3 g/l, its critical micelle concentration (cmc) was 150 mg/l, showing a better surface activity than chemical surfactant ... | 2010 | 21072942 |
| distal-to-proximal no conversion in hemoproteins: the role of the proximal pocket. | hemoproteins play central roles in the formation and utilization of nitric oxide (no) in cellular signaling, as well as in protection against nitrosative stress. key to heme-nitrosyl function and reactivity is the fe coordination number (5 or 6). for (five-coordinate) 5c-no complexes, the potential for no to bind on either heme face exists, as in the microbial cytochrome c' from alcaligenes xylosoxidans (axcytcp), which forms a stable proximal 5c-no complex via a distal six-coordinate no interme ... | 2010 | 21073879 |
| effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan. | chitosan is a biopolymer with antimicrobial activity and film-forming properties. in this study, the effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan was evaluated. a chitosan was selected from eight types (four non-commercial and four commercial) based on its antimicrobial activity against salmonella enterica serovar enteritidis (s. enteritidis). for this purpose, a contact plate method was developed and chitosans were applied at a concen ... | 2010 | 21146239 |
| optimization of nitrilase production from alcaligenes faecalis mtcc 10757 (iict-a3): effect of inducers on substrate specificity. | microbial nitrilases are biocatalysts of interest and the enzyme produced using various inducers exhibits altered substrate specificity, which is of great interest in bioprocess development. the aim of the present study is to investigate the nitrilase-producing alcaligenes faecalis mtcc 10757 (iict-a3) for its ability to transform various nitriles in the presence of different inducers after optimization of various parameters for maximum enzyme production and activity. the production of a. faecal ... | 2010 | 21188422 |
| [in vitro antimicrobial activity of piperacillin-sulbactam and other 7 antimicrobial agents against non-fermentative bacilli]. | to evaluate the in vitro antimicrobial activity of piperacillin-sulbactam against clinical isolates of non-fermentative bacilli isolated from common infections. | 2010 | 21211358 |
| dddy, a periplasmic dimethylsulfoniopropionate lyase found in taxonomically diverse species of proteobacteria. | the abundant compatible solute dimethylsulfoniopropionate (dmsp) is made by many marine algae. different marine bacteria catabolise dmsp by various mechanisms, some of which liberate the environmentally important gas dimethyl sulfide (dms). we describe an enzyme, dddy, which cleaves dmsp into dms plus acrylate and is located in the bacterial periplasm, unlike other dmsp lyases that catalyse this reaction. there are dddy-like genes in strains of alcaligenes, arcobacter and shewanella, in the β-, ... | 2011 | 21248856 |
| [characteristics of proteins synthesized by hydrogen-oxidizing microorganisms]. | the study was conducted to determine the biological value of proteins synthesized by hydrogen-oxidizing microorganisms--the hydrogen bacteria alcaligenes eutrophus z1 and ralstonia eutropha b5786 and the co-resistant strain of carboxydobacterium seliberia carboxydohydrogena z1062. based on a number of significant parameters characterizing the biological value of a product, the proteins of hydrogen-oxidizing microorganisms have been found to occupy an intermediate position between traditional ani ... | 2010 | 21261071 |
| comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries. | the preparation of enantiopure tertiary alcohols is of great contemporary interest due to the application of these versatile building blocks in organic synthesis and as precursors towards high value pharmaceutical compounds. herein, we describe two approaches taken towards the discovery of novel biocatalysts for the synthesis of these valuable compounds. the first approach was initiated with screening of 47 bacterial strains for hydrolytic activity towards the simple tertiary alcohol ester tert- ... | 2011 | 21318363 |
| immunostimulatory activities of a decapeptide derived from alcaligenes faecalis fy-3 to crucian carp. | a strain was isolated from a soil sample collected from weihe river in shaanxi province (108°03'e 34°14'n), which was identified as alcaligenes faecalis by 16s rrna analysis. a compound m showing potent immune activity was isolated from secondary metabolites of the strain through bioassay-guided isolation techniques. the structure of the compound m was elucidated using ft-ir, ei-ms, 1h nmr and 13c nmr spectra and identified as cyclo-(l-pro-gly)5 which was first time reported as a natural product ... | 2011 | 21332568 |
| bacterial community composition characterization of a lead-contaminated microcoleus sp. consortium. | a microcoleus sp. consortium, obtained from the ebro delta microbial mat, was maintained under different conditions including uncontaminated, lead-contaminated, and acidic conditions. | 2011 | 21340467 |
| [significance of evaluation of the antibiotic-resistant properties of bacterial producing strains in the hygienic standardization system]. | the study was undertaken to evaluate the antibiotic sensitivity of microorganisms that were used in biotechnological production and belonged to different taxons: gram-positive (bacillus subtilis, bacillus licheniformis, and bacillus thuringiensis) and gram-negative (alcaligenes denitrificans and pseudomonas caryophylii) bacteria and noncardioform actinomycetes (rhodococcus erythropolis and rhodococcus corallinus). the sensitivity of the strains to a range of antibiotics was determined by the aga ... | 2010 | 21344698 |
| [biodiversity of mesophilic microbial community bynd-8 capability of lignocellulose degradation and its effect on biogas production]. | the biodiversity of a mesophilic microbial community bynd-8 capable of degrading lignocellulose at 30 degrees c was detected using denaturing gradient gel electrophoresis (dgge) and the isolation of pure cultures, and the effect of the liquid of rice straw degradation by bynd-8 on biogas production was measured. six bacterial strains were isolated using peptone cellulose solution medium, and the highest similarities of their 16s rdna gene sequences to serratia sp. psgb 13, s. marcescens strain u ... | 2011 | 21404695 |
| a case of polyglandular autoimmune syndrome type i with unusual presentation. | eight years old girl presented with mucocutaneous candidiasis, nail dystrophy, twitching left half of face, progressively increasing generalized skin hyperpigmentation and hypopigmented patches over both shins. her investigations revealed low intact pth level, low serum cortisol, high acth, impaired glucose tolerance test and candidal onycomycosis. she was diagnosed as polyglandular autoimmune syndrome (pga) type i. she also developed idiopathic generalized epileptiform seizures and alcaligenes ... | 2011 | 21419032 |
| turbiscan lab ® expert analysis of the biological demulsification of a water-in-oil emulsion by two biodemulsifiers. | the long-term destabilization process of a water-in-oil emulsion was investigated with two different biodemulsifiers produced under different culture conditions by alcaligenes sp. s-xj-1. biodemulsifier i was obtained by using paraffin as substrate at initial culture ph of 10 and biodemulsifier ii was produced with waste frying oils at ph of 7. the former exhibited higher demulsifying ability and interfacial activity than the latter. bottle test, microscopy and turbiscan lab(®) expert were used ... | 2011 | 21458159 |
| isolation and characterization of an alcaligenes sp. strain dg-5 capable of degrading ddts under aerobic conditions. | in this study, an alcaligenes sp. strain dg-5 that can effectively degrade dichlorodiphenyltrichloro-ethanes (ddts) under aerobic conditions was isolated from ddts-contaminated sediment. various factors that affect the biodegradation of ddts by dg-5 were investigated. about 88 %, 65 % and 45 % of the total ddts were consumed within 120 h when their initial concentrations were 0.5, 5 and 15 mg l?¹, respectively. however, almost no degradation was observed when their concentration was increased to ... | 2011 | 21462053 |
| surface properties of bacteria from activated sludge in relation to bioflocculation. | two bacterial stains were isolated from the activated sludge and identified as leucobacter sp. and alcaligenesfaecalis by 16s rdna sequencing. pure cultures of these two strains, representing well or poorly settled bacteria, were used to investigate the mechanism of bioflocculation in activated sludge. based on the analyses of the characteristics of cells hydrophobicity, zeta-potential, flocculation ability and extracellular polymeric substance (eps) composition under different growth stages, it ... | 2010 | 21462699 |
| proton-coupled electron transfer in the catalytic cycle of alcaligenes xylosoxidans copper-dependent nitrite reductase. | we demonstrated recently that two protons are involved in reduction of nitrite to nitric oxide through a proton-coupled electron transfer (et) reaction catalyzed by the blue cu-dependent nitrite reductase (cu nir) of alcaligenes xylosoxidans (axnir). here, the functionality of two putative proton channels, one involving asn90 and the other his254, is studied using single (n90s, h254f) and double (n90s-h254f) mutants. all mutants studied are active, indicating that protons are still able to reach ... | 2011 | 21469743 |
| unusual causes of peritonitis in a peritoneal dialysis patient: alcaligenes faecalis and pantoea agglomerans. | an 87 -year-old female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. with the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients. | 2011 | 21477370 |
| cell-based biosensor for measurement of phenol and nitrophenols toxicity. | a cost-effective whole cell biosensor based on electrochemical technique to detect toxicities of phenol and nitrophenols has been developed. this method relied on the inhibition effect for respiratory chain activity of microorganism by toxicant, which was measured by chronoamperometry using mediator (ferricyanide). the current signals produced by suspended microorganisms and reoxidation of ferrocyanide were transformed to inhibiting efficiency directly, and 50% inhibiting concentration (ic(50)) ... | 2011 | 21482280 |
| visualization and direct counting of individual denitrifying bacterial cells in soil by nirk-targeted direct in situ pcr. | the abundance of denitrifying bacteria in soil has been determined primarily by the conventional most probable number (mpn) method. we have developed a single-cell identification technique that is culture-independent, direct in situ pcr, to enumerate denitrifying bacteria in soils. the specificity of this method was evaluated with six species of denitrifying bacteria using nirk as the target gene; escherichia coli was used as a negative control. almost all (97.3%-100%) of the nirk-type denitrify ... | 2011 | 21487206 |
| engineering the substrate specificity of alcaligenesd-aminoacylase useful for the production of d-amino acids by optical resolution. | d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6 (axd-naase) offers a novel biotechnological application, the production of d-amino acid from the racemic mixture of n-acyl-dl-amino acids. however, its substrate specificity is biased toward certain n-acyl-d-amino acids. to construct mutant axd-naases with substrate specificities different from those of wild-type enzyme, the substrate recognition site of the axd-naase was rationally manipulated based on computational structural ... | 2011 | 21546325 |
| polyphasic approach for assessing changes in an autochthonous marine bacterial community in the presence of prestige fuel oil and its biodegradation potential. | a laboratory experiment was conducted to identify key hydrocarbon degraders from a marine oil spill sample (prestige fuel oil), to ascertain their role in the degradation of different hydrocarbons, and to assess their biodegradation potential for this complex heavy oil. after a 17-month enrichment in weathered fuel, the bacterial community, initially consisting mainly of methylophaga species, underwent a major selective pressure in favor of obligate hydrocarbonoclastic microorganisms, such as al ... | 2011 | 21562979 |
| a novel synthesis of iminodiacetic acid: biocatalysis by whole alcaligenes faecalis zjb-09133 cells from iminodiacetonitrile. | iminodiacetic acid (ida) has been widely used as an important intermediate in the fine chemical industry. in this study, a novel synthesis route of ida from iminodiacetonitrile by whole microorganisms was investigated. a strain with the capability of producing nitrilase, zjb-09133, was isolated and identified, and later named alcaligenes faecalis zjb-09133. in addition, the detailed biocatalysis of iminodiacetonitrile to produce ida using zjb-09133 was investigated. the results showed that the c ... | 2011 | 21567989 |
| removal of heavy metals using different polymer matrixes as support for bacterial immobilisation. | great attention is focused on the microbial treatment of metal contaminated environments. three bacterial strains, 1c2, 1zp4 and ec30, belonging to genera cupriavidus, sphingobacterium and alcaligenes, respectively, showing high tolerance to zn and cd, up to concentrations of 1000ppm, were isolated from a contaminated area in northern portugal. their contribution to zn and cd removal from aqueous streams using immobilised alginate, pectate and a synthetic cross-linked polymer was assessed. in mo ... | 2011 | 21592655 |
| reverse-transcriptase quantitative pcr method to detect uptake of hydrogen produced from cyanobacteria by alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium. | hydrogen-oxidising bacteria play a key ecological role in a variety of habitats including the rhizosphere and hot springs. to investigate the possibly of interspecies hydrogen exchange between cyanobacteria and hydrogen-oxidising bacteria, we developed a sensitive and reliable reverse-transcriptase qpcr assay for up-regulation of the hups gene in the knallgas bacterium alcaligenes hydrogenophilus dsm2625. the assay detected up-regulation of the gene at initial hydrogen concentrations as low as 0 ... | 2011 | 21617984 |
| fatal catheter-related bacteremia due to alcaligenes (achromobacter) xylosoxidans in a hemodialysis patient. | | 2011 | 21637989 |
| screening and characterization of potential cadmium biosorbent alcaligenes strain from industrial effluent. | cadmium (cd) is a heavy metal that is easily accumulated in the living organisms in connection with anthropogenic activities which may result in serious health problems. in the present study, five potential cadmium tolerant bacterial strains were isolated from industrial effluent with heavy metal contamination and were screened for biosorption potential with their active growth in different media. after growth in shake flasks containing mineral salt media and tryptone soya broth, cell pellet of ... | 2011 | 21656817 |
| effects of entrapment on nucleic acid content, cell morphology, cell surface property, and stress of pure cultures commonly found in biological wastewater treatment. | the effects of cell entrapment on nucleic acid content, cell morphology, cell surface property, and stress of major groups of bacteria (betaproteobacteria and gammaproteobacteria) in biological municipal wastewater treatment were investigated. three different entrapment media (alginate, carrageenan, and polyvinyl alcohol) were examined. results indicated that the entrapment and type of entrapment media affected nucleic acid content, cell morphology, cell surface property, and stress of the three ... | 2011 | 21660542 |
| emerging gram-negative infections in burn wounds. | gram-negative infection remains a major contributor to morbidity, mortality, and cost of care. in the absence of comparative multinational epidemiological studies specific to burn patients, we sought to review literature trends in emerging gram-negative burn wound infections within the past 60 years. mapping trends in these organisms, although in a minority compared with the six "eskape" pathogens currently being targeted by the infectious diseases society of north america, would identify pathog ... | 2011 | 21792068 |
| characterization and biodegradation of polycyclic aromatic hydrocarbons in radioactive wastewater. | pah degrading pseudomonad and alcaligenes species were isolated from landfill soil and mine drainage in south africa. the isolated organisms were mildly radiation tolerant and were able to degrade pahs in simulated nuclear wastewater. the radiation in the simulated wastewater, at 0.677bq/µl, was compatible to measured values in wastewater from a local radioisotope manufacturing facility, and was enough to inhibit metabolic activity of known pah degraders from soil such as pseudomonas putida gmp- ... | 2011 | 21782341 |
| biodegradation of tributyl phosphate by novel bacteria isolated from enrichment cultures. | tributyl phosphate (tbp) is an organophosphorous compound, used extensively (3000-5000 tonnes/annum) as a solvent for nuclear fuel processing and as a base stock in the formulation of fire-resistant aircraft hydraulic fluids and other applications. because of its wide applications and relative stability in the natural environment tbp poses the problem of pollution and health hazards. in the present study, fifteen potent bacterial strains capable of using tributyl phosphate (tbp) as sole carbon a ... | 2011 | 21755325 |
| surface microbial consortia from livarot, a french smear-ripened cheese. | the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ... | 2011 | 21815832 |
| enrichment of co2-fixing bacteria in cylinder-type electrochemical bioreactor with built-in anode compartment. | bacterial assimilation of co2 into stable biomolecules using electrochemical reducing power may be an effective method to reduce atmospheric co2 without fossil fuel combustion. for the enrichment of the co2-fixing bacteria using electrochemical reducing power as an energy source, a cylinder-type electrochemical bioreactor with a built-in anode compartment was developed. a graphite felt cathode modified with neutral red (nr-graphite cathode) was used as a solid electron mediator to induce bacteri ... | 2011 | 21715965 |
| cell growth and p(3hb) accumulation from co(2) of a carbon monoxide-tolerant hydrogen-oxidizing bacterium, ideonella sp. o-1. | cell growth and accumulation of polyhydroxybutyric acid, p(3hb), from co(2) in autotrophic condition of a newly isolated hydrogen-oxidizing bacterium, the strain o-1, was investigated. the bacterium, which was deposited in the japan collection of microorganisms as jcm17105, autotrophically grows by assimilating h(2), o(2), and co(2) as substrate. 16s rrna gene sequence of the bacterium was the closest to ideonella dechloratans (99%). specific growth rate of the strain o-1 was faster than a hydro ... | 2011 | 21695533 |
| production optimization of cyanophycinase chpeal from pseudomonas alcaligenes dip1. | abstract: pseudomonas alcaligenes dip1 produces an extracellular cyanophycinase (cpheal). the corresponding gene (cpheal) was identified from subclones of a genomic dna gene library by heterologously expressing the functionally active enzyme in escherichia coli. the nucleotide sequence of the gene (1260 base pairs) was determined indicating a theoretical mass of 43.6 kda (mature cpheal) plus a leader peptide of 2,6 kda which corresponds well to the apparent molecular mass of 45 kda as revealed b ... | 2011 | 22060187 |
| Effects of associated bacteria on the pathogenicity and reproduction of the insect-parasitic nematode Rhabditis blumi (Nematoda: Rhabditida). | Three bacteria, Alcaligenes faecalis , Flavobacterium sp., and Providencia vermicola , were isolated from dauer juveniles of Rhabditis blumi . The pathogenic effects of the bacteria against 4th instar larvae of Galleria mellonella were investigated. Providencia vermicola and Flavobacterium sp. showed 100% mortality at 48 h after haemocoelic injection, whereas A. faecalis showed less than 30% mortality. Dauer juveniles showed 100% mortality against G. mellonella larvae, whereas axenic juveniles, ... | 2011 | 21867444 |
| Exploiting metagenomic diversity for novel polyhydroxyalkanoate synthases: Production of a terpolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) with a recombinant Pseudomonas putida strain. | A metagenomic library of 2.1×10(6) clones was constructed using oil-contaminated soil from Gujarat (India). One of the fosmid clones, 40N22, encodes a polyhydroxyalkanoate synthase showing 76% identity with an Alcaligenes sp. synthase. The corresponding gene was expressed in Pseudomonas putida KT2440 ?phaC1 which is impaired in PHA production. The gene conferred the recombinant strain PpKT-40N22 with the ability to produce copolymers with up to 21% in medium-chain-length content. Thus, 37% and 4 ... | 2012 | 22071242 |
| Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity. | Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium contai ... | 2011 | 22131949 |
| Spoilage potentials and antimicrobial resistance of Pseudomonas spp. isolated from cheeses. | Pseudomonas spp. are aerobic, gram-negative bacteria that are recognized as major food spoilage microorganisms. A total of 32 (22.9%) Pseudomonas spp. from 140 homemade white cheese samples collected from the open-air public bazaar were isolated and characterized. The aim of the present study was to investigate the biochemical characteristics, the production of extracellular enzymes, slime and ß-lactamase, and antimicrobial susceptibility of Pseudomonas spp. isolated from cheeses. The identified ... | 2011 | 22118075 |
| redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase. | single-molecule measurements are a valuable tool for revealing details of enzyme mechanisms by enabling observation of unsynchronized behavior. however, this approach often requires immobilizing the enzyme on a substrate, a process which may alter enzyme behavior. we apply a microfluidic trapping device to allow, for the first time, prolonged solution-phase measurement of single enzymes in solution. individual redox events are observed for single molecules of a blue nitrite reductase and are use ... | 2011 | 21969548 |
| Remediation of oil-based drill cuttings through a biosurfactant-based washing followed by a biodegradation treatment. | In this study, oil-based drill cuttings were washed by a rhamnolipid solution and then subjected to bioremediation in stainless steel boxes using sawdust as bulking agent. A mixed bacterial culture, mainly containing Pseudomonas, Acinetobacter, Alcaligenes, Agrobacterium, and Comamonas, was used as inoculums. Approximately 83% of organics were removed after washing under optimal conditions (liquid/solid ratio, 3:1; washing time, 20 min; stirring speed, 200 rpm; rhamnolipid concentration, 360 mg/ ... | 2011 | 21925873 |
| hessian fly-associated bacteria: transmission, essentiality, and composition. | plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ... | 2011 | 21858016 |
| assessment of denitrifying bacterial composition in activated sludge. | the abundance and structure of denitrifying bacterial community in different activated sludge samples were assessed, where the abundance of denitrifying functional genes showed nirs in the range of 10(4)-10(5), nosz with 10(4)-10(6) and 16s rrna gene in the range 10(9)-10(10) copy number per ml of sludge. the culturable approach revealed pseudomonas sp. and alcaligenes sp. to be numerically high, whereas culture independent method showed betaproteobacteria to dominate the sludge samples. comamon ... | 2011 | 21868215 |
| [bioaugmentation of bioreactors with a pjp4 receiving transconjugant to enhance the removal of 2,4-d]. | the paper first investigated horizontal transfer of a conjugative plasmid pjp4 to two pure strains of e. coli dh5alpha and alcaligenes sp., and a mixed culture of aerobic granular sludge, respectively. with a pjp4 receiving transconjugant alcaligenes sp. :: pjp4 as the bioaugmented bacteria, bioaugmentation experiments were conducted in an aerobic granular sludge reactor and a biofilm reactor, respectively, to enhance the removal of a recalcitrant compound 2,4-dichlorophenoxyacetic acid(2,4-d). ... | 2011 | 21922845 |
| spectroelectrochemical investigation of intramolecular and interfacial electron-transfer rates reveals differences between nitrite reductase at rest and during turnover. | a combined fluorescence and electrochemical method is described that is used to simultaneously monitor the type-1 copper oxidation state and the nitrite turnover rate of a nitrite reductase (nir) from alcaligenes faecalis s-6. the catalytic activity of nir is measured electrochemically by exploiting a direct electron transfer to fluorescently labeled enzyme molecules immobilized on modified gold electrodes, whereas the redox state of the type-1 copper site is determined from fluorescence intensi ... | 2011 | 21863850 |
| indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance. | introduction: transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. the purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora. methods: microorganisms in pristine and oily soils wer ... | 2011 | 21948132 |
| biodegradation and detoxification of melanoidin from distillery effluent using an aerobic bacterial strain sag5 of alcaligenes faecalis. | distillery effluent retains very dark brown color even after anaerobic treatment due to presence of various water soluble, recalcitrant and coloring compounds mainly melanoidins. in laboratory conditions, melanoidin decolorizing bacteria was isolated and optimized the cultural conditions at various incubation temperatures, ph, carbon sources, nitrogen sources and combined effect of both carbon and nitrogen sources. the optimum decolorization (72.6 ± 0.56%) of melanoidins was achieved at ph 7.5 a ... | 2011 | 21880418 |
| evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders. | investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ... | 2011 | 22205979 |