| [a case of klebsiella bacteremia with brain, lung, and liver and skin abscesses]. | | 1988 | 2457061 |
| characterization of nifh mutations of klebsiella pneumoniae. | nucleotide changes in the nifh gene of klebsiella pneumoniae were identified by dna cloning and sequencing of six selected mutant strains. the strains were un60, c-640-gc----tgc; un116, c-67-tc----ttc; un117, g-688-ag----aag; un1041, cg-302-c----cac; un1678, gc-713-c----gtc; and un1795, g-439-ag----aag. their corresponding amino acid substitutions were un60, arg-214----cys; un116, leu-23----phe; un117, glu-230----lys; un1041, arg-101----his; un1678, ala-238----val; and un1795, glu-147----lys. re ... | 1988 | 2457577 |
| autoimmune pathogenesis for ankylosing spondylitis (as) and reiter's syndrome (rs): autoantibodies against an epitope shared by hla b27 and klebsiella pneumoniae nitrogenase in sera of hla b27 patients with as and rs. | | 1987 | 2457977 |
| the pho-controlled outer membrane porin phoe does not contain specific binding sites for phosphate or polyphosphates. | purified phoe-porins were reconstituted into black lipid bilayer membranes, and the selectivity and size of the reconstituted pores were determined. addition of polyphosphates influenced the internal charge situation of the pore resulting in a shift from anion to cation selectivity. however, the pore size as judged from single channel conductances was not influenced by the addition of polyphosphates. a strong inhibition of the pore conductance only occurred when mg2+ was also present in the aque ... | 1988 | 2458341 |
| immunological comparison of constitutive beta-lactamases of gram-negative bacteria by neutralization in zymogram gels: properties of anti-tem-1 and anti-tem-2 sera. | the zymogram technique was applied to a beta-lactamase neutralization assay with anti-tem-1 and anti-tem-2 sera. both were shown to contain neutralizing antibodies directed towards various beta-lactamases of gram-negative bacteria. the quantitative neutralization allowed classification into five groups of the 28 beta-lactamases used as standards and 61 from clinical isolates. in the first were enzymes such as tem-1 and tem-2 including tle-1, shv-1, shv-2, penicillinases of klebsiella pneumoniae ... | 1988 | 2460115 |
| effect of macromolecular composition of microorganisms on the thermodynamic description of their growth. | most models describing bacterial growth, including the original mosaic non-equilibrium thermodynamic (mnet) description, do not take into account that the macromolecular composition of the cells varies with growth rate. the mnet description of bacterial growth is extended to account for such a variation in macromolecular composition of the cells in order to make the mnet description more generally applicable. klebsiella aerogenes nctc 418 was cultured in a chemostat under glucose- or ammonia-lim ... | 1988 | 2461738 |
| monoclonal antibodies against the capsular k antigen of escherichia coli (o9:k30(a):h12): characterisation and use in analysis of k antigen organisation on the cell surface. | monoclonal antibodies were produced against the capsular antigen of escherichia coli serotype k(a)30, using a mouse hybridoma system. the antibodies also recognised the chemically identical capsular polysaccharide produced by klebsiella k20. chemical modification of the k30 polysaccharide indicated that the glucuronic acid residues found in the e. coli k30 capsular antigen were important in the epitope recognised by these antibodies. use of the antibodies as molecular probes revealed the presenc ... | 1988 | 2461792 |
| molecular mimicry between human leukocyte antigen b27 and klebsiella. consequences for spondyloarthropathies. | ankylosing spondylitis and reiter's syndrome are the two major spondyloarthropathies highly associated with human leukocyte antigen (hla) b27. although the development of spondylitis is unclear, it has been hypothesized that hla-b27 may predispose to spondyloarthropathies via the phenomenon of molecular mimicry. a computer search for homologies between hla-b27 and microbes revealed a sequence of six consecutive amino acids (glutamine-threonine-aspartic acid-arginine-glutamic acid-aspartic acid) ... | 1988 | 2462350 |
| cross-reacting bacterial determinants in ankylosing spondylitis. | the importance of the association between the human histocompatibility antigen human leukocyte antigen-b27 and ankylosing spondylitis is undisputed, but its biologic significance remains unresolved. we have demonstrated specific cross reactivity between a range of enteric bacteria and a specific determinant found only on the surfaces of cells from human leukocyte antigen-b27-positive persons with ankylosing spondylitis. we have proposed that the genetic element coding for this cross-reactive det ... | 1988 | 2462351 |
| post-ercp acute necrotizing pancreatitis. | an analysis of acute necrotizing pancreatitis (anp) after endoscopic retrograde cholangiopancreatography (ercp) was carried out. the incidence of anp was 0.5% (5/914) for ercp and 0.5% (2/370) for endoscopic sphincterotomies (est). all the five patients were obese, middle-aged or older women. four had a suspicion of common bile duct stones and the fifth a pancreatic tumour as an indication for ercp. two had most probably a functional sphincteric disorder and the third was without clear pathologi ... | 1988 | 2462849 |
| transfer and expression of the genetic determinants for o and k antigen synthesis in escherichia coli o9:k(a)30 and klebsiella sp. o1:k20, in escherichia coli k12. | escherichia coli serotype o9:k(a)30 and klebsiella o1:k20 produce thermostable capsular polysaccharides or k antigens, which are chemically and serologically indistinguishable. plasmid pulb113 (rp4::mini-mu) has been used to mediate chromosomal transfer from e. coli o9:k30 and klebsiella o1:k20 to a multiply marked, unencapsulated, e. coli k12 recipient. analysis of the cell surface antigens of the transconjugants confirmed previous reports that the genetic determinants for the e. coli k(a) anti ... | 1988 | 2463068 |
| human monocytes exposed to biostim (ru 41740) alter lymphocyte mitogenesis: mechanisms of action. | the immunomodulatory agent ru 41740 (biostim), which is derived from klebsiella pneumoniae, may augment mitogenic responses of purified human blood lymphocytes. in non-purified preparations, however, responses may be sharply reduced due to the fact that biostim induces monocytes to secrete immunosuppressive factors. this investigation has shown that both these biological activities can be exerted by a single, major glucoprotein fraction of biostim termed f1. the biostim-induced suppression of mi ... | 1988 | 2466802 |
| cross-reactive epitope with klebsiella pneumoniae nitrogenase in articular tissue of hla-b27+ patients with ankylosing spondylitis. | synovial tissues from patients with ankylosing spondylitis or reactive arthritis were examined by an immunoperoxidase technique, using antisera to synthetic peptides representing antigens shared between hla-b27.1 and klebsiella pneumoniae nitrogenase. with either antiserum, all hla-b27+ patients with synovial inflammation showed strong immunoperoxidase staining in synovial lining cells, vascular endothelium, and infiltrating inflammatory cells. these findings indicate that antigens showing cross ... | 1989 | 2468338 |
| characteristics and mechanism of action of a heat-stable enterotoxin produced by klebsiella pneumoniae from infants with secretory diarrhea. | escherichia coli heat-stable enterotoxins (st) are classified into sta and stb according to their physicochemical and biologic characteristics. sta induces diarrhea, activating the guanylate cyclase-cgmp system. st-like enterotoxins can be produced by bacteria other than e. coli, including klebsiella pneumoniae. a klebsiella st has previously been shown to share some chemical and immunologic characteristics with e. coli st. aiming to define better the nature of klebsiella st, we have screened 23 ... | 1989 | 2470015 |
| synovial expression of hla-b27 related antigens in ankylosing spondylitis. | | 1988 | 2471998 |
| the influence of the o-antigen and the capsular polysaccharide on the establishment of plcmts lysogeny in klebsiella pneumoniae. | the o-antigen of the lipopolysaccharide in klebsiella pneumoniae caused a significant reduction in the frequency of establishment of plcmts lysogeny, while the capsular polysaccharide showed no effect on this frequency. the bacterial receptor for plcmts are the lipopolysaccharide-core oligosaccharides, the results suggest that k. pneumoniae strains with an o-antigen in their lipopolysaccharide have a poorly accessible lipopolysaccharide-core (the plcmts bacterial receptor), while k. pneumoniae s ... | 1989 | 2477305 |
| [media for determining the dnaase of klebsiella]. | a modified medium is suggested for measuring klebsiella dnaase activity; the advantage of this medium is the ability of maximally producing the enzyme. the medium is simple to prepare and may be widely used in practical bacteriology. | 1989 | 2477634 |
| [preventive immunoprevention of rhinitis and sinusitis. ent director dr. w. elies, bielefeld, elaborates on the indications]. | | 1989 | 2478431 |
| a high-molecular-weight fraction of smooth lipopolysaccharide in klebsiella serotype o1:k20 contains a unique o-antigen epitope and determines resistance to nonspecific serum killing. | the lipopolysaccharide (lps) o-antigen side chains of klebsiella serotype o1 have been studied by using mutants selected by resistance to a klebsiella bacteriophage designated o1-a. two classes of lps mutants were identified. the major group (90%) synthesized rough lps. the remaining 10% of the mutants produced a novel lps profile that lacked the highest-molecular-weight o-substituted molecules (hmw-lps) but still produced lower-molecular-weight o-substituted species (lmw-lps). by using antisera ... | 1989 | 2478478 |
| [study of the adhesive properties of klebsiella and their evaluation]. | the study has been aimed at selection of the optimal blood group of humans for obtaining red cells to be used in the system for the studies of klebsiella adhesion characteristics. the results evidence that any human blood group is fit for it. examinations of 95 klebsiella strains have shown the informative parameters of adhesion and the criteria for the assessment of these parameters. | 1989 | 2481082 |
| sequence homology between putative raw-starch binding domains from different starch-degrading enzymes. | | 1989 | 2481445 |
| hospital-acquired neonatal bacterial meningitis: the impacts of cefotaxime usage on mortality and of amikacin usage on incidence. | all cases of bacterial meningitis in the neonatal unit at king edward viii hospital, durban for the period 1 january 1981 to 31 december 1987 were reviewed. in particular, we looked at the impact of cefotaxime on mortality rates and amikacin on the incidence of hospital-acquired gram-negative bacillary (gnb) meningitis. klebsiella was found to be the commonest cause of neonatal meningitis, followed by escherichia coli and streptococcus agalactiae. eighty-four per cent of all cases of gnb meningi ... | 1989 | 2482005 |
| use of a bacteriophage-encoded glycanase enzyme in the generation of lipopolysaccharide o side chain deficient mutants of escherichia coli o9:k30 and klebsiella o1:k20: role of o and k antigens in resistance to complement-mediated serum killing. | coliphage k30 lysates contain free and phage-associated forms of a bacteriophage-encoded capsule depolymerase (glycanase) enzyme, active against the serotype k30 capsular polysaccharide of escherichia coli. the free glycanase has been purified to apparent homogeneity. the molecular weight of the enzyme was estimated at 450,000, and when heated in sds at 100 degrees c, the enzyme dissociated into two subunits of 90,000 and 52,000. the glycanase enzyme was used as a reagent to reversibly degrade t ... | 1989 | 2482125 |
| molecular mimicry--a chimera? | | 1989 | 2483175 |
| [gram-negative flora of horticultural produce destined for consumption mainly in the raw state]. | a survey has been carried out to evaluate the recovery of enterobacteriaceae in freshly consumed horticultural products. 64 samples of these vegetables random chosen in different stores in the general vegetable market of ferrara have been examined among the families of compositae (lettuce, prickly lettuce, cabbage lettuce, common chicory, artichoke), umbrelliferae (curly parsley, carrot, celery, fennel), cruciferae (garden cabbage, red radish), liliaceae (onion), and solanaceae (tomato). 654 bac ... | 1989 | 2483908 |
| production of monoclonal antibodies against surface antigenic determinants of klebsiella pneumoniae. | the production of four murine monoclonal antibodies (kp26, kp53, kp62 and kp71) to klebsiella pneumoniae surface antigen(s) is described. the binding of all four monoclonal antibodies to k. pneumoniae was inhibited by f(ab')2 fragments of normal human serum igg, suggesting that the antigenic determinants of k. pneumoniae detected by the four monoclonal antibodies may be similar to those recognized by human serum igg. the antigen identified by kp62 was purified from a deoxycholate-solubilized bac ... | 1988 | 2485091 |
| [the bacteriological control of tpn mixtures at the hospital de bellvitge]. | the complications of sepsis often make it necessary to suspend nutritional therapy. the origin of these is the nutritive mixture as well as the catheter connection and/or point where it has been inserted. the aim of this study is to show the need for bacteriological control of the whole process, in order to evaluate the effectiveness of the methods used, dilucidate the origin of the sepsis and establish an internal quality control. study of different methods for bacteriological control of the nu ... | 1989 | 2485355 |
| phage typing of klebsiella strains in epidemiological studies. | a set of 14 routinely applied bacteriophages was used for the typing of klebsiella pneumoniae and klebsiella oxytoca strains isolated from patients in 1983-1986. the phage-sensitive strains (65%) differed in respect to the place and time of isolation which manifested itself by an occurrence of specific lysotypes in wrocław, gdańsk, wernigerode and włocławek, as well as various repeatibility on the lysotypes within 10 years. | 1989 | 2487377 |
| antibiotic resistance and phage types of faecal klebsiella isolated from healthy zoo apes. | the isolated species of klebsiella were characterized by biotyping and phage typing methods. the majority of strains were resistant to certain examined drugs. for epidemiological reasons these strains can be potentially dangerous to the population of zoo animals. | 1989 | 2487378 |
| [activity of cefpirome against bacteria isolated from hospitalized patients]. | the activity of cefpirome (hr 810) was evaluated against 247 strains isolated from patients developing their infections while in a hospital in buenos aires. its activity against gram negative bacilli was compared with ceftriaxone, ceftazidime, cephalotin, piperacillin, amikacin, gentamicin and norfloxacin. in terms of mic50 and mic90 (mg/l) it was as follows: klebsiella pneumoniae: less than 0.125, less than 2.0; pseudomonas aeruginosa: less than 8, less than 16; escherichia coli: less than 0.01 ... | 1989 | 2487748 |
| evaluation of copovithane as a nonspecific immunomodulator in surgically simulated sepsis. | copovithane (cpv), a synthetic polymer, has been shown to have antitumor activity and also to reduce mortality in experimental murine peritonitis. the purpose of this study was to compare cpv with muramyl dipeptide (mdp), an immunomodulator of proven efficacy in simulated surgical infection. six groups of cba/j mice were compared; they received intramuscular injections of normal saline (controls), mdp (100 micrograms), or cpv (100, 200, and 400 mg/kg) 24 h prior to bacterial challenge. the chall ... | 1989 | 2488006 |
| [usefulness of 5 microbiological markers for the differentiation of strains of klebsiella ozaenae causing an outbreak of nosocomial infection]. | five different microbiological markers (biotyping, antimicrobial susceptibility, heavy-metal susceptibility, klebocin susceptibility and number of extrachromosomal dna fragments) were utilized in order to distinguish between klebsiella ozaenae strains arising from a nosocomial infection outbreak and strains of some species isolated from hospital environment. antimicrobial susceptibility pattern to 13 different antibiotics was the most reliable maker, because 85% of the outbreak strains shown res ... | 1989 | 2488767 |
| [susceptibility of clinically-isolated problem pathogenic bacteria to broad spectrum antibiotics]. | the action of broad-spectrum antibiotics investigated by disc diffusion method on 636 problem bacteria such as s. aureus, enterobacter, klebsiella, pseudomonas, e. coli, proteus that isolated from various clinical specimens. the highest and lowest ratio of susceptibility were as follows: s. aureus were found sensitive 90% to netilmicin, 27% to piperacillin, enterobacter species 78% to netilmicin, 25% to piperacillin, klebsiella 69% to amikacin, 20% to piperacillin, pseudomonas 66% to amikacin, 5 ... | 1989 | 2488944 |
| [antibiotic sensitivity of strains of klebsiella isolated in geneva and algiers]. | 573 strains of klebsiella were isolated in geneva (239 strains) and in algiers (334 strains). the complete work with bacteriologic features (culture, biochemistry and antigenicity) will be published later. two investigations took place in surgical cardio-vascular service in geneva cantonal hospital and in algiers hospitals: pediatric (beni-messous) and intensive care service (mustapha). high resistance percentages are found with penicillins (greater than 60% by c.m.i.) in geneva and algiers. the ... | 1989 | 2489399 |
| [resistance to cephalosporin in hospital strains of the kes group]. | in our laboratory kes group bacteria account for about 11% of all strains isolated from in-patients and are responsible for serious infections. their well known increasing tendency to become resistant to beta-lactams prompted us to the kes strains isolated in medical or surgical patients in 1986.87. 30 klebsiella retrospectively review the susceptibility to antibiotics of strains out of 59 had been isolated in that period from patients previously treated with beta-lactams: no significant variati ... | 1989 | 2490396 |
| [epidemiologic and therapeutic aspects of bacteremic infections of the urinary tract]. | a survey was carried out of all urinary tract infections which developed bacteremia during an 18 month period. the number of episodes of bacteremia of urinary origin (buo) detected in our institution during that period was 108. the urinary tract was the most common origin of the cases of community acquired (ca) bacteremia and the third in the hospital acquired (ha) cases. in both types, the most frequent organism was e. coli (69.6% and 40%, respectively). the most common underlying diseases were ... | 1989 | 2490670 |
| [liver abscess caused by klebsiella pneumoniae associated with septic endophthalmitis]. | | 1989 | 2490689 |
| [chronic pneumonia with a 4-year course]. | | 1989 | 2490713 |
| [evaluation of the treatment with oral ciprofloxacin: experience with 24 patients]. | we have evaluated the clinical effectiveness and possible side effects of oral ciprofloxacin therapy in 24 patients (11 females and 13 males). two episodes from overall 25 infections were excluded of clinical and microbiological evaluation but were assessed regarding possible side effects. in most patients, the clinical condition was stable at the beginning of therapy. only 3 patients had nonfatal diseases. seventeen episodes were urinary tract infections (four of them with concomitant bacteremi ... | 1989 | 2490722 |
| [nosocomially acquired infection of the urinary tract]. | we have evaluated 283 consecutive hospital acquired urinary tract infections (hauti) in a university hospital (incidence 5.6% of admissions). in females, spontaneous, symptomatic and younger patient infections predominated, while in males hauti were mostly asymptomatic, after catheterization and in elderly patients. chronic nonfatal diseases--particularly neurologic disease and diabetes--, old age, previous antibiotic use, the postoperative period, and cancer were the major general predisposing ... | 1989 | 2490855 |
| [liver abscesses due to klebsiella pneumoniae and acute anterior uveitis]. | liver abscesses are not very frequent and those produced by klebsiella spp. are quite exceptional. these germs have been related to the development of acute anterior uveitis (aau) in patients whose susceptibility has been linked to hla b27. we present a case of a female, hla b27 negative, who developed aau during a course of liver abscesses caused by klebsiella pneumoniae. | 1989 | 2491048 |
| immunomodulation of polymorphonuclear leukocytes by d53 immucytal and its constitutive fractions. | d53 (immucytal) is a compositive vaccine made of immunogenic ribosomes extracted from 4 bacterial species (klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes and streptococcus pneumoniae) associated with a membrane proteoglycan from a non-encapsulated strain of klebsiella pneumoniae (mpg-kp). in this work we have studied the effect of the compound on human polymorphonuclear leukocyte (pmn) function "in vitro". we have demonstrated that d53 was able to significantly increase fc ... | 1989 | 2491432 |
| [significant bacteriological findings in hiv-positive patients]. | twenty-four episodes of bacterial infections were identified over a 18 month period in 11 patients (8 with acquired immunodeficiency syndrome and 3 with aids related complex). eight of the 11 infected patients were drug abusers and 3 homosexual people. nosocomial bacterial infections were common in patients with aids and had high fatality rates. gram-negative bacteria resulted the most common micro-organisms (e.coli, proteus, enterobacter, serratia, klebsiella). the aztreonam treatment was very ... | 1989 | 2491436 |
| expression and nucleotide sequence of the lactobacillus bulgaricus beta-galactosidase gene cloned in escherichia coli. | the lactobacillus bulgaricus beta-galactosidase gene was cloned on a ca. 7-kilobase-pair hindiii fragment in the vector pkk223-3 and expressed in escherichia coli by using its own promoter. the nucleotide sequence of the gene and approximately 400 bases of 3'- and 5'-flanking sequences was determined. the amino acid sequence of the beta-galactosidase, deduced from the nucleotide sequence of the gene, yielded a monomeric molecular mass of ca. 114 kilodaltons, slightly smaller than the e. coli lac ... | 1989 | 2492511 |
| mechanisms of bacterial opsonization by immune globulin intravenous: correlation of complement consumption with opsonic activity and protective efficacy. | to study the mechanism of bacterial opsonization by immune globulin intravenous (igiv) complement consumption and polymorphonuclear leukocyte (pmnl) membrane receptor (fcrlo, cr1, and cr3)-mediated phagocytosis of staphylococcus epidermidis, klebsiella pneumoniae, and groups a and b streptococci were examined. igiv alone did not consume complement and showed no opsonic activity by itself for these organisms. when these bacteria were preopsonized in igiv, significant amounts of complement were co ... | 1989 | 2494269 |
| evolution of chemotactic-signal transducers in enteric bacteria. | the methyl-accepting chemotactic-signal transducers of the enteric bacteria are transmembrane proteins that consist of a periplasmic receptor domain and a cytoplasmic signaling domain. to study their evolution, transducer genes from enterobacter aerogenes and klebsiella pneumoniae were compared with transducer genes from escherichia coli and salmonella typhimurium. there are at least two functional transducer genes in the nonmotile species k. pneumoniae, one of which complements the defect in se ... | 1989 | 2496104 |
| enhancing effect of serum ultrafiltrate on the activity of cephalosporins against gram-negative bacilli. | a few studies have suggested that the inhibitory effect of serum on activity of broad-spectrum cephalosporins is less than that predicted by the degree of protein binding. microdilution mics of ceftriaxone, cefoperazone, moxalactam, and ceftizoxime were therefore determined against atcc and clinical strains of escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, and staphylococcus aureus in mueller-hinton broth containing either human albumin (as 0, 2.5, or 5% solution) or heat-inact ... | 1989 | 2496656 |
| [therapeutic effectiveness of an intravenous immunoglobulin with ph 4.25 in experimental neonatal sepsis caused by klebsiella pneumoniae]. | the in vitro opsonic activity and in vivo therapeutic effect of an intravenous immunoglobulin (igiv) ph 4.25 against klebsiella pneumoniae were evaluated in this study. by an opsonophagocytic assay in microtiter plates, bacteria were opsonized with igiv ph 4.25, 10% rabbit serum, or 10% rabbit serum heated at 56 degrees c for 30 minutes. opsonized bacteria were challenged with polymorphonuclear leukocytes (pmns) from normal adults and bacterial killing was measured at 60 and 150 minutes. forty-f ... | 1989 | 2496700 |
| [primary macroglobulinemia with severe neutropenia, leading to a rapid development of septic shock]. | a 88-year-old man was admitted because of the left chest pain due to herpes zoster for 1 week. blood analyses and immunoelectrophoresis revealed anemia, severe neutropenia, rouleaux formation and igm, lambda-type monoclonal gammopathy. the he staining and peroxidase-anti-peroxidase staining of biopsy specimens of the cervical lymph node swelling appeared from the fifth hospital day, revealed an increase in atypical lymphocytes bearing igm, lambda-type immunoglobulin. then a diagnosis of primary ... | 1989 | 2497264 |
| [the rapid identification of e. coli by the detection of 3 enzymes]. | 232 isolates of gram-negative, oxidase-negative bacteria, isolated from various samples of different animal species, were tested with help of rapidec coli for the production of beta-glucuronidase, beta-galactosidase and indole. the test correctly identified 164 of 175 e. coli strains (sensitivity 93.7%) and correctly indicated that 57 of 57 isolates of the family enterobacteriaceae (7 citrobacter sp., 18 enterobacter sp., 16 klebsiella sp., 10 proteus sp., 2 providencia sp. and 4 salmonella sp.) ... | 1989 | 2497726 |
| synthesis of novel 5-fluoro analogues of norfloxacin and ciprofloxacin. | a series of polyfluoro-3-quinolonecarboxylic acids have been synthesized and their in vitro antibacterial activity evaluated. the desired 7-(substituted amino) derivatives were prepared from the 5,6,7,8-tetrafluoroquinolone acids. conversely, amine displacement occurred primarily at the 5-position when the ester was used. structure-activity studies indicated that the antibacterial activity was greatest when the n-1 substituent was cyclopropyl and the 7-substituent was 4-methyl-1-piperazinyl. all ... | 1989 | 2498519 |
| [antagonistic action of bacillus subtilis cultures on bacteria of the genus klebsiella]. | | 1989 | 2498623 |
| [antibiotic resistance of gram-negative bacteria isolated at a hospital intensive care unit]. | the antibiotic sensitivity of gram-negative facultatively pathogenic bacteria isolated between 1982-1986 in the respiratory intensive care unit of the 3rd department of pediatry of the "lászló" hospital (budapest) has been studied. pseudomonas aeruginosa, klebsiella, acinetobacter calcoaceticus and acinetobacter lwoffii were most frequently isolated both from the clinical samples (total 56.9%) and in samples obtained from hospital-hygienic check-examinations. 70-100% of the individual species wa ... | 1989 | 2498804 |
| [prevention of dysbacteriosis in the early neonatal period using a pure culture of acidophilic bacteria]. | in order to prevent dysbacterioses occurring in the early neonatal period, 60 neonates classified with the risk group were administered, when they were still at the puerperal room, pure cultures of specially selected strains of lactobacillus acidophilus possessing marked anti-klebsiella activity and antagonistic activity against staphylococcus aureus. the cultures were instilled into the mouth and nasal passages. forty neonates administered bifidum-bacterin and 54 neonates not given biopreparati ... | 1989 | 2498834 |
| in-vitro susceptibility of nosocomial gram-negative bloodstream pathogens to quinolones and other antibiotics--a statistical approach. | we examined the in-vitro activity of 12 antibiotics against gram-negative bacillary isolates from 141 distinct episodes of nosocomial bloodstream infection occurring from july 1984 through november 1986. at least ten strains of each of the seven most frequently encountered species were tested. relative potency was carefully assessed by extending the concentrations from 0.004 to 64 mg/l in microdilution tests performed in duplicate. we estimated mic50 and mic90 and, importantly, calculated 95% co ... | 1989 | 2499564 |
| opsonic activity of a new intravenous immunoglobulin preparation: pentaglobin compared with sandoglobulin. | standard preparations of immunoglobulin for intravenous use consist predominantly of igg (greater than 95%). we have compared the ability of a standard preparation (sandoglobulin) with that of a new preparation (pentaglobin, containing 12% igm and 12% iga) to improve the opsonic activity of antibody-deficient human sera in vitro. panhypogammaglobulinaemic sera were poorly opsonic for five of six organisms tested, particularly pseudomonas aeruginosa, escherichia coli and streptococcus pneumoniae, ... | 1989 | 2500275 |
| bacteriological study of genital tract infection in puerperium. | of 185 vaginal and 13 wound swabs obtained from febrile females after delivery and abortions, 140 (75.6%) vaginal and 10 (76.9%) wound swabs were positive for bacteria. infection caused by a single bacterium was found in 100 (71.4%) and mixed infection in 40 (28.5%). e. coli was mainly isolated followed by staph. aureus and klebsiella. fosfomycin was the most effective antibiotic against e. coli and gentamycin against staph. aureus. | 1989 | 2500542 |
| [plasmids and genetic determinants of antibiotic resistance of gram-negative bacteria]. | distribution of plasmids and genetic determinants of antibiotic resistance was studied in 129 strains of pseudomonas, klebsiella, serratia and enterobacter isolated from oncological patients. it was shown that 56 isolates contained the plasmids, 9 conjugative plasmids being plasmids with broad bacterial host spectrum. a significant part of the strains contained genes controlling production of aph (3"), type ii aph (3'), type i and ii dhps and type type ii dhfr. genetic determinants of tetracycli ... | 1989 | 2500901 |
| bacteriological study of choledochal bile in patients with common bile duct stones, with or without acute suppurative cholangitis. | acute suppurative cholangitis is a serious complication in extrahepatic biliary tract obstruction. bacteriological studies have shown that in these patients the bile cultures usually present several kinds of bacteria, especially gram-negative aerobic bacteria such as e. coli and klebsiella sp. the mortality rate in our patients with common bile duct stones and acute suppurative cholangitis is enhanced 5- to 10-fold. the aim of the present study was to analyse the bacterial properties of choledoc ... | 1989 | 2502489 |
| rapid impedance detection of salmonellas in confectionery using modified licnr broth. | a commercially available broth, with the addition of inhibitors, was used for the rapid impediometric detection of salmonellas in confectionery. pre-enrichment in skimmed milk was followed by lysine-iron-cystine-neutral red broth in a bactometer 123 system. results were obtained 3 d earlier than is possible with conventional microbiological tests. some false positives were obtained predominantly with citrobacter freundii, but this problem is also frequently encountered with traditional methods. ... | 1989 | 2502528 |
| kinetics of ethanolic fermentation of d-xylose by klebsiella pneumoniae and its mutants. | the microbial production of ethanol from d-xylose by a new soil isolate of klebsiella pneumoniae and the mutants k. pneumoniae mb-16 and mb-16-1048 was studied. kinetic and physiological properties of the mutants were compared with those of the original isolate. the volumetric rates of ethanol formation by mutants mb-16-1048 and mb-16 and the original isolate were 1.58, 0.50, and 0.06 g liter-1 h-1, respectively. the cultivation times of mutants mb-16-1048 and mb-16 were 20 and 18 h, respectivel ... | 1989 | 2502944 |
| contaminated enteral nutrition solutions as a cause of nosocomial bloodstream infection: a study using plasmid fingerprinting. | in july 1984, two patients fed enteral nutrition solutions contaminated with enterobacter cloacae developed nosocomial bacteremia. despite careful review of the preparation procedures as well as repeated microbiological surveys, 83 (27%) of the 309 formula bottles tested over a 1-yr period were contaminated and the source of contamination remained unknown. e. cloacae was the most frequent organism isolated (34%). the plasmid profiles of e. cloacae recovered from enteral nutrition solutions remai ... | 1989 | 2503632 |
| effect of nucleotides on the activity of dinitrogenase reductase adp-ribosyltransferase from rhodospirillum rubrum. | the mechanism by which mgadp stimulates the activity of dinitrogenase reductase adp-ribosyltransferase (drat) has been examined by using dinitrogenase reductases from rhodospirillum rubrum, klebsiella pneumoniae, and azotobacter vinelandii as acceptor substrates. in the presence of 0.2 mm nad, maximal rates of adp-ribosylation of all three acceptors were observed at an adp concentration of 150 microm; in the absence of added adp, drat activity with the dinitrogenase reductases from r. rubrum and ... | 1989 | 2504283 |
| serum bactericidal activity against enterobacteriaceae producing broad-spectrum beta-lactamases in volunteers administered ofloxacin and cefotaxime, alone or combined. | the activity of ofloxacin and cefotaxime, alone or combined, against four strains of enterobacteriaceae was evaluated both in vitro and in sera from volunteers given a single infusion over 30 min of 200 mg ofloxacin or 1 g cefotaxime. the strains showed resistance or decreased susceptibility to third-generation cephalosporins. the combination was not found to be synergistic in vitro. analysis of the bactericidal titres and killing kinetics of sera taken at the time of the peak concentration and ... | 1989 | 2504593 |
| comparative activity of cefixime and cefaclor in an in vitro model simulating human pharmacokinetics. | the dependence of the antibacterial activity of the two oral cephalosporins cefixime and cefaclor on pharmacokinetic properties was investigated in an in vitro model using strains of enterobacteria and a streptococcal strain. in the cultures the course of serum concentrations of the respective antibiotic was simulated. the more rapidly attained (1 h) high peak levels (17.5 micrograms/ml) of cefaclor (500 mg dose) in no case showed an advantage over the more slowly reached (3 h) low peak levels ( ... | 1989 | 2504600 |
| surveillance of antibiotic resistance in south east asia. | antibiotic resistance in gram-negative bacteria, particularly salmonella and shigella, requires surveillance worldwide. this study describes results of surveys in hong kong, bangkok and kuala lumpur. all strains were isolated in hospitals which have large community catchment areas in addition to specialised hospital units. the prevalence of resistant strains was high in all areas. gram-negative bacteria such as enterobacter associated with hospital infections were resistant to penicillins and ce ... | 1989 | 2504618 |
| identification and characterization of the nifh and nifj promoter regions located on the nif-plasmid pea3 of enterobacter agglomerans 333. | small restriction fragments of the plasmid-borne enterobacter agglomerans 333 nif region were cloned into a promoter probe plasmid as transcriptional fusions with the lacz gene. identification of nifa-dependent promoters was accomplished by using a compatible plasmid which constitutively expresses the klebsiella pneumoniae nifa gene. beta-galactosidase assays showed strong activation of the cloned e. agglomerans promoters in escherichia coli by the heterologous k. pneumoniae nifa gene product. t ... | 1989 | 2504647 |
| purple urine bags. | purple urine drainage bags were found in 7 of 71 chronically catheterized elderly women. the purple staining of the bags is due to a violet discoloration (indirubin) of the plastic of the catheter bag and fine blue crystals of indigo in the urine. the colors are formed from the substrate indoxyl sulfate (indican) and all 7 patients had bacteria in the urine that would produce blue colonies on agar enriched with the urine (filter sterilized) of the patients involved. organisms identified were pro ... | 1989 | 2504940 |
| urinary tract infection. | over two years, 9892 mid-stream urine samples from patients attending the aga khan university hospital, karachi were cultured. significant bacterial growth was seen in 23.5% samples. further identification of these organisms revealed 40% of e. coli, 16% pseudomonas aeruginosa, 11% klebsiella aerogenes, 5.0% enterobacter sp., 13% proteus sp., 4.0% serratia liquifaciens, 1.0% acinetobacter sp., 3.0% citrobacter sp., 4.0% enterococci, 0.5% staphylococcus aureus. results of sensitivity tests perform ... | 1989 | 2504948 |
| synergy of fluoroquinolones with other antimicrobial agents. | synergy resulting from the combination of ciprofloxacin and an antipseudomonal penicillin has been reported for 20%-50% of isolates of pseudomonas aeruginosa. a similar effect has also been reported for the combination of ciprofloxacin and fosfomycin. in contrast, the combination of quinolones and aminoglycosides rarely showed synergy when tested against p. aeruginosa. most studies have shown that the combination of beta-lactams or aminoglycosides with quinolones is indifferent against escherich ... | 1989 | 2505355 |
| growth of gram-positive and gram-negative bacteria in platelet concentrates. | in 1986 the allowable platelet storage time was reduced from 7 to 5 days because of a recent increase in septic deaths associated with platelet transfusion. in this study, the growth curves of two gram-positive and two gram-negative organisms in platelets stored for 7 days in clx and pl-732 bags were evaluated. platelets in clx bags were inoculated with 10(1), 10(2), and 10(3) organisms and 10(2) organisms were introduced into pl-732 bags. test organisms were inoculated into trypticase soy broth ... | 1989 | 2505412 |
| studies on the emergence of resistance to lomefloxacin in vitro. | the emergence of resistance to lomefloxacin, norfloxacin and ciprofloxacin was investigated in nalidixic acid sensitive and resistant urinary isolates by continuous turbidimetry. a decline in susceptibility was observed after a single exposure to each of the drugs, and further increments of resistance occurred during three sequential passages. variants resistant to one quinolone were cross-resistant to the others. the level of resistance selected by norfloxacin in three of the five test strains ... | 1989 | 2506046 |
| [a case of septic shock due to pneumonia caused by klebsiella pneumoniae]. | a 51-year-old male, who had a history of excessive drinking and chronic hepatitis, was admitted to our hospital because of high fever and shock. physical examinations, chest x-ray films and hemodynamic data revealed that he had progressed to septic shock due to pneumonia. combination chemotherapy of latamoxef plus piperacillin was immediately started. after klebsiella pneumoniae was isolated from bronchoalveolar lavage fluid, the antibiotics were changed to ceftizoxime plus amikacin. furthermore ... | 1989 | 2506304 |
| [clinical studies on septicemia and infective endocarditis encountered between 1985-1986]. | septicemia encountered at kawasaki municipal hospital between 1985 and 1986 were studied clinically. forty six patients had monomicrobial and 5 has polymicrobial infections, respectively. out of these 46 patients with septicemia, 17 were due to escherichia coli, 7 were due to klebsiella pneumoniae and 4 were due to staphylococcus aureus. ten patients had hepatobiliary, 7 had hematological, 7 had malignant diseases as underlying diseases, respectively. out of 10 patients complicated with septic s ... | 1989 | 2506308 |
| [bactericidal synergy of josamycin and human polynuclear neutrophils in vitro]. | due to their high intracellular uptake, macrolides may interfere with phagocytes antibacterial system. we have studied the interaction of josamycin with bactericidal activity of human neutrophils (pmns) or whole blood, in vitro. pmns preincubated with josamycin (100-0.1 mg/l) display an increased phagocytic ability for k. pneumoniae, independently of the concentrations of josamycin; this effect appears to correlate with the recruitment of an additional population of phagocytizing pmns without al ... | 1989 | 2506509 |
| [antibacterial activity of pefloxacin in the urine during the 7 days following a single 800 mg oral dose]. | antibacterial activity of pefloxacin was studied in the urine after a single 800 mg oral dose in ten healthy female volunteers. urine was collected in 9 periods: 0-6 h, 6-12 h, 12-24 h, 24-28 h, 48-72 h, 72-96 h, 96-120 h, 120-144 h, 144-168 h. pefloxacin concentrations were assayed in all samples by a microbiological method and by hplc. urine antibacterial activity was determined towards five bacterial strains isolated in urine: 2 e. coli strains, one sensitive and the other resistant to nalidi ... | 1989 | 2506514 |
| [antibacterial activity of lomefloxacin in the urine during the 4 days following a single 400 mg oral dose]. | antibacterial activity of lomefloxacin was studied in the urine after single dose of 400 mg in ten healthy female volunteers. urine was collected in 7 periods: 0-3 h, 3-6 h, 6-12 h, 12-24 h, 24-48 h, 48-72 h, 72-96 h. lomefloxacin concentration were assayed in all samples by microbiological method. urine antibacterial activity was determined towards five strains isolated in urine: 2 e. coli strains one sensitive and the other resistant to nalidixic acid (nal-a), 1 klebsiella pneumoniae resistant ... | 1989 | 2506515 |
| [studies on 145 cases of septicemia in infancy and childhood--especially on septicemic patients with malignant and hematological diseases]. | clinical and bacteriological data of 145 inpatients with septicemia, treated at the hospital of chiba university school of medicine from 1972 to 1987, were reviewed by dividing them into three stages. (stages i: 1972-76, stage ii: 1978-82, stage iii: 1983-87) patients with underlying diseases have been increasing: 91.8% of the total patients in stage iii. among the patients with underlying diseases, malignant and hematological diseases occupied about 60%, and in the other diseases, congenital he ... | 1989 | 2507684 |
| [susceptibility of clinical isolates to aztreonam]. | in vitro antibacterial activities of 9 antibiotics including aztreonam (azt) against clinically isolated gram-negative bacteria were determined using mic-2000 plus system. bacteria were isolated from clinical materials in saga medical school during a period from may 1987 to march 1988. summarized results were as follows: 1. azt showed excellent antibacterial activities against escherichia coli, klebsiella pneumoniae, proteus sp. and haemophilus influenzae, and mic80 values of azt against these o ... | 1989 | 2507799 |
| [in vitro antibacterial effect of a new oral cephalosporin, cefixime. results of a multicenter study]. | minimal inhibitory concentrations (mic) of cefixime (cxm) were evaluated by agar dilution against 2,469 bacterial strains isolated in 10 hospitals. for enterobacteriaceae, mic 50 and 90% micrograms/ml were respectively: (i) naturally non beta lactamase producing species: e. coli and shigella 0.25-0.5; salmonella 0.06-0.25; p. mirabilis 0.008-0.032. (ii) chromosomal penicillinase producing species: klebsiella 0.06-2. (iii) chromosomal cephalosporinase producing species: e. cloacae and c. freundii ... | 1989 | 2508039 |
| effect of concentration of substrates and products on the growth of klebsiella pneumoniae in chemostat cultures. | non-equilibrium thermodynamics (net) can be used to describe microbial growth. in this description, the concentrations of products contribute to the driving forces of the metabolic processes (anabolism and catabolism). thus, in contrast to the model of bacterial growth of monod (recherches sur la croissance les cultures bactériennes (1942) herman et cie, paris), it is predicted that the growth rate of a bacterial chemostat culture is, in principle, dependent on the concentration of the catabolic ... | 1989 | 2508755 |
| in-vitro activity of meropenem imipenem, the penem hre 664 and ceftazidine against clinical isolates from west germany. | the antibacterial activity in vitro of meropenem was compared with another carbapenem, imipenem, the penem hre 664, and ceftazidime. mics were not influenced by ph nor by inoculum size below 5 x 10(5) cfu/ml: higher inocula caused a modest increase in mic. cation supplementation of mueller-hinton broth was without effect. meropenem was more active than imipenem and the other comparative agents against the majority of gram-negative species (in particular proteus, providencia, morganella, haemophi ... | 1989 | 2509418 |
| [study on bacterial infection by the investigation of antimicrobial susceptibility of various clinical isolates. iii. comparison of drug susceptibility between the isolates from the materials of out- and in-patients, and among the isolates from the materials of different hospital units]. | antimicrobial agents susceptibility of 42, 940 strains of clinical isolates (from 1979 to 1986) were investigated and the data were analyzed on the basis of the sources of isolates; materials from in- and out-patients or from the different hospital units. bacteria studied were limited to the species of which isolates were 100 or more during 1979 to 1986. the following results were obtained. i) when the antibiotic susceptibility of the isolates from the out-patient-materials were compared with th ... | 1989 | 2509589 |
| [production of oxygen free radicals by phagocytes in patients on hemodialysis]. | in order to elucidate the vulnerability to infection in patients on chronic hemodialysis, as one of the host defense mechanisms, the production of oxygen free radicals by phagocytes was studied in patients by luminol- or lucigenine-enhanced chemiluminescence (cl). whole blood cl of the patients, in both luminol- or lucigenine-enhanced was significantly higher than that in healthy adults after stimulation by zymosan, pma, and staphylococcus aureus (s. aureus) 209 p. however, the cl response of th ... | 1989 | 2509599 |
| saudi arabian-american differences in antimicrobial resistance of escherichia, klebsiella, and pseudomonas. | increasing microbial resistance to newly developed antibiotics has been a limiting factor in the therapeutic use of these agents. to determine the extent of the problem, in vitro antimicrobial susceptibility of 7,140 clinical isolates of escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa to seven commonly used antibiotics was established at the 1,700-bed riyadh central hospital in saudi arabia and compared with 5,513 isolates at the oklahoma memorial hospital and veterans admini ... | 1989 | 2509721 |
| effect of bacterial lipopolysaccharide on growth of murine bladder cancer, mbt-2. | intradermal palpable mbt-2 tumor responded with bacterial lipopolysaccharide to hemorrhagic necrosis (lps) in c3h/hen (endotoxin sensitive) mice. we have tested lps fractions isolated from e. coli, klebsiella pneumoniae, salmonella minnesota, pseudomonas aeruginosa and serratia culture filtrates. all these lps preparations showed tumor necrotizing activity accompanied by toxicity (body weight loss) in c3h/hen mice. however, mbt-2 tumors grown in an endotoxin-resistant strain (c3h/hej) of mice di ... | 1989 | 2510388 |
| bacteriological investigations and resistance patterns in beef and goat's meat in colombo's slaughter-house. | a high contamination rate was found in meat of slaughtered cattle and goats at the time of this study. certain organisms were isolated from meat and also from wounds of slaughter workers. a high antibiotic resistance pattern was found - in some instances. following observations of high surface meat contamination, associated with high antibiotic resistance, it must be assumed, that these highly resistant organisms can also be directly transmitted to humans by transport, transaction and by food pr ... | 1989 | 2511074 |
| analysis of infection in a burn ward. | one hundred and seventy-two burn wound swabs obtained from 90 patients admitted to the aljila hospital burn unit, benghazi over a 3-month period were processed to determine the microbial flora colonizing burns and their resistance patterns to selected locally available topical and systemic agents. approximately 84.9 per cent of the swab specimens yielded growth of 11 bacterial species and candida spp.; of these, ps. aeruginosa, staph. aureus and klebsiella spp. predominated in order of prevalenc ... | 1989 | 2511853 |
| impact of the dosage schedule on the efficacy of ceftazidime, gentamicin and ciprofloxacin in klebsiella pneumoniae pneumonia and septicemia in leukopenic rats. | the impact of the dosage schedule on the therapeutic efficacy of antibiotics was investigated in experimental klebsiella pneumoniae pneumonia and septicemia in leukopenic rats. the daily doses (mg/kg) that protected 50% of the animals from death, when calculated for administration at 6 h intervals or by continuous infusion, were as follows: ceftazidime 24.4 and 1.5 (p less than 0.001), gentamicin 2.8 and 3.8 (p greater than 0.05), and ciprofloxacin 3.3 and 6.5 (p less than 0.05), respectively. t ... | 1989 | 2512132 |
| comparative in vitro activity of the new oral penem alp-201 against aerobic and anaerobic bacteria. | the in vitro activity of the new penem derivative alp-201 against 226 aerobic and 350 anaerobic clinical bacterial isolates was determined using agar dilution techniques. for comparison amoxicillin, cefaclor, ceftazidime, doxycycline, erythromycin, imipenem and trimethoprim/sulfamethoxazole were also tested with aerobic bacteria, and cefoxitin, chloramphenicol, clindamycin, imipenem, metronidazole and piperacillin with anaerobic bacteria. alp-201 was found to be highly active against escherichia ... | 1989 | 2512142 |
| protective effects of recombinant human tumour necrosis factor alpha and interferon gamma against surgically simulated wound infection in mice. | tumour necrosis factor alpha and interferon gamma have both been shown to have immunoregulatory properties, and to be able to influence, several microbial infections. this study showed that tumour necrosis factor was effective in modifying surgically simulated wound infections when administered both as prophylaxis and as therapy. two models were used; one was an intramuscular bacterial challenge, and the other involved the use of a bacteria-laden thigh suture. the test bacterium for both models ... | 1989 | 2514003 |
| in vitro activity of dactimicin, a novel pseudodisaccharide aminoglycoside, compared with activities of other aminoglycosides. | the in vitro activity of dactimicin, a new pseudodisaccharide aminoglycoside which possesses a formimidoyl group, was compared with those of gentamicin, tobramycin, and amikacin against 500 isolates. dactimicin inhibited 90% of isolates from the family enterobacteriaceae at a concentration of less than or equal to 4 micrograms/ml. it was more active than amikacin against klebsiella pneumoniae, serratia marcescens, citrobacter diversus, enterobacter agglomerans, yersinia species, and salmonella s ... | 1989 | 2514625 |
| [kinetics of the antibacterial effect of ampicillin and sulbactam combinations in dynamic and static conditions]. | kinetics of antimicrobial effect (ame) of ampicillin/sulbactam combinations (ratios of 4:1 to 1:2) on ampicillin resistant bacterial strains producing beta-lactamases of types ii, iii, iv and v according to richmond classification was studied with using the computerized system ms-2 (turbidimetric recording) and an in vitro dynamic model (microcalorimetric recording). the concentrations of the drugs in system ms-2 (static conditions) corresponded to the maximum ones observed in serum of humans af ... | 1989 | 2514630 |
| antibacterial activity and beta-lactamase stability of mdl 19,592, an oral cephalosporin. | we compared the in vitro activity and beta-lactamase stability of mdl 19,592, an orally absorbed cephalosporin, with that of cephalexin and cefaclor. it inhabited staphylococcus aureus at less than or equal to 4 micrograms/ml, streptococcus pyogenes at 0.25 microgram/ml, sero groups b, c and g streptococci at 1 microgram/ml, and streptococcus pneumoniae at 2 micrograms/ml. it was slightly more active than cefaclor and cephalexin. mdl 19,592 did not significantly inhibit enterobacteriaceae, enter ... | 1989 | 2515027 |
| dna sequence analysis of artificially evolved ebg enzyme and ebg repressor genes. | the ebg system has been used as a model to study the artificial selection of new catalytic functions of enzymes and of inducer specificities of repressors. a series of mutant enzymes with altered catalytic specificities were previously characterized biochemically as were the changes in inducer specificities of mutant, but fully functional, repressors. the wild type ebg operon has been sequenced, and the sequence differences of the mutant enzymes and repressors have been determined. we now report ... | 1989 | 2515108 |
| clonal analysis of the lac operons from klebsiella m5al and the lac plasmid (pre2) from klebsiella v9a. | the chromosomal lac region of the coliform bacterium klebsiella m5al was cloned into the multicopy plasmid pbr322 to give phe7 and phe8. phe8 contains 12.6 kb of m5al dna, including its complete lac operon, and phe7 contains 2.5 kb of m5al dna and includes the complete lac y gene and a small segment of lacz. the m5al operon has the same gene order as the escherichia coli lac operon. the lac genes of the lac plasmid of klebsiella v9a were cloned into pbr322 to give phe1 and phe2, of approximately ... | 1989 | 2515112 |
| [a clinical study of terminal pneumonia]. | a total of 210 cases of terminal pneumonia were studied out of 1183 autopsied cases at tenri yorozu hospital from 1978 to 1985. underlying diseases included lung cancer (77 patients), gastric cancer (26 patients), leukemia (24 patients). there was no statistical significance between the time from death until autopsy and the bacterial examination of autopsied lung and blood. p. aeruginosa and klebsiella sp. were the most frequently isolated organisms. seventy percent of isolated organisms were gr ... | 1989 | 2515333 |
| functional expression of a rhodospirillum rubrum gene encoding dinitrogenase reductase adp-ribosyltransferase in enteric bacteria. | the function of the cloned drat gene of rhodospirillum rubrum was studied by placing it under the control of the tac promoter in the vector, pkk223-3. after induction with isopropyl-beta-d-thiogalactopyranoside, dinitrogenase reductase adp-ribosyltransferase (drat) activity was detected in crude extracts of the heterologous hosts escherichia coli and klebsiella pneumoniae. in addition, the expression of drat produced a nif- phenotype in the otherwise wild-type k. pneumoniae strains, the result o ... | 1989 | 2515993 |
| long-term experience with 1 per cent topical silver sulphadiazine cream in the management of burn wounds. | three hundred and forty-two patients with 10-50 per cent body surface area burns were studied prospectively over the 5-year period from 1982 to 1986 for the effectiveness of topical 1 per cent silver sulphadiazine. various parameters were studied including: (i) healing time of deep partial skin thickness burns, (ii) eschar separation time, (iii) conversion rate of deep dermal burns to full skin thickness burns, (iv) burn wound surface bacterial flora and their changing pattern over the years, (v ... | 1989 | 2516445 |
| immunoprotective extracellular polysaccharides of klebsiella aerogenes capsular type k1, expressed in escherichia coli. | a gene library of genomic dna klebsiella aerogenes of capsular serotype k1 was constructed in e. coli le392 using the cosmid pmmb33. culture filtrates of e. coli recombinants were screened by elisa for extracellular polysaccharides specific for k. aerogenes k1. extracellular polysaccharide extracts from k. aerogenes k1 and 3% of the e. coli recombinants contained immunoprotective extracellular polysaccharides (iep) with similar chemical and immunological properties as shown by gel filtration thr ... | 1989 | 2517220 |