| a comparative study of avian bordetella-like strains, bordetella bronchiseptica, alcaligenes faecalis and other related nonfermentable bacteria. | cultural and biochemical characteristics, electrophoretic patterns of soluble proteins, and pathogenicity in turkey poults, of 30 avian gram-negative nonfermentable bacterial strains acquired from various geographical regions, of which 26 had been isolated from turkeys and one each from a chicken, duck, goose and sharp-tailed munia, were compared to those of bordetella bronchiseptica, alcaligenes faecalis, alcaligenes denitrificans, achromobacter xylosoxidans and bordetella parapertussis. with t ... | 1983 | 18766782 |
| experimental studies on turkey coryza. | disease with severe respiratory symptoms, nasal discharge, swelling of sinuses, conjunctivitis with foamy discharge and mortality has been observed in turkey flocks in israel. of the pathogenic agents isolated from field cases, three, paramyxovirus, yucaipa (yucaipa virus), alcaligenes faecalis (a. faecalis) and mycoplasma gallisepticum (mg) were used to infect turkey poults, singly or in combination, in our attempt to reproduce the field disease symptoms. yucaipa virus alone caused mild symptom ... | 1984 | 18766831 |
| body temperature response of turkey poults infected with alcaligenes faecalis. | experiments were conducted to investigate the colonic temperature (tc) response to alcaligenes faecalis infection in turkeys. one-day-old poults were inoculated intranasally with 0.05 ml of a 24 hour brain heart infusion (bhi) broth culture with a bacterial concentration of 1.0 x 10(9) cells/ml. within 2 hours after inoculation tc was depressed significantly in challenged poults. this depression was significant through day 14 in experiment 1 and day 6 in experiment 2. further studies with steril ... | 1984 | 18766888 |
| the effect of combined and separate infection by alcaligenes faecalis and paramyxovirus (yucaipa) on the surface morphology of the trachea in turkey poults. | sixty-seven 1-day-old turkey poults were inoculated in the infra orbital sinus with 3 x 10(8) alcaligenes faecalis bacteria and/or by 10(5.8)eid 50/ ml of yucaipa virus. twenty-five similar birds served as controls. identification of the agents inoculated was made during the development of disease by means of isolation and serological tests. during the development of the disease, the surface morphology of the trachea in the affected animals revealed cellular oedema associated with a mucus coatin ... | 1985 | 18766948 |
| effects of ph on the activity and structure of choline oxidase from alcaligenes species. | a reversible effect of ph on the ionization of amino-acid residues at the active center of choline oxidase was observed near the optimum ph (8). inactivation of choline oxidase took place in the ph ranges 3-6 and 9-11, in which irreversible changes in the structure occur leading to the enzyme inactivation. the first order rate constants of the enzyme's inactivation at various ph values were estimated for the irreversible changes. the arrhenius analysis revealed no significant changes in the acti ... | 2008 | 18769736 |
| amphoteric surfactant n-oleoyl-n-methyltaurine utilized by pseudomonas alcaligenes with excretion of n-methyltaurine. | the amphoteric surfactant n-oleoyl-n-methyltaurine, which is in use in skin-care products, was utilized by aerobic bacteria as the sole source of carbon or of nitrogen in enrichment cultures. one isolate, which was identified as pseudomonas alcaligenes, grew with the xenobiotic compound as the sole source of carbon and energy. the sulfonate moiety, n-methyltaurine, was excreted quantitatively during growth, while the fatty acid was dissimilated. the initial degradative reaction was shown to be h ... | 2008 | 18783436 |
| evaluation of screening methods for demulsifying bacteria and characterization of lipopeptide bio-demulsifier produced by alcaligenes sp. | in this paper, surface tension measurement, oil-spreading test and blood-plate hemolysis test were attempted in the screening of demulsifying bacteria. after the comparison to the screening results obtained in demulsification test, 50 mn/m of surface tension of culture was proposed as a preliminary screening standard for potential demulsifying bacteria. for the identification of efficient demulsifying strains, surface tension level was set at 40 mn/m. the detected strains were further verified i ... | 2009 | 18799309 |
| [enhanced production of curdlan by alcaligenes faecalis by selective feeding with ammonia water during the cell growth phase of fermentation]. | curdlan is a water insoluble exopolysaccharide produced by alcaligenes faecalis under nitrogen-limiting conditions. after excretion, the polysaccharide is attached the cell wall. thus enhancement of biomass production during the cell growth phase is important to curdlan production. a strategy of increasing nitrogen source to improve biomass production was adopted for curdlan production by alcaligenes faecalis (atcc 31749). in the batch fermentation of curdlan, a relatively higher nh4cl level of ... | 2008 | 18807988 |
| the missing link: bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic bordetellae. | bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus bordetella. phylogenetically, it connects the pathogenic bordetellae and environmental bacteria of the genera achromobacter and alcaligenes, which are opportunistic pathogens. b. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. recently, clinical isolates asso ... | 2008 | 18826580 |
| physico-chemical and microbial characteristics of the coral reef environment of the gulf of mannar marine biosphere reserve, india. | investigation on physico-chemical parameters and bacteial characteristics of the coral reef environs of the gulf of mannar biosphere reserve was studied. the study found the influence of different physico-chemical parameters on one another and also on the distribution of the total heterotrophic bacteria (thb) in the coral reef areas. nutrients exhibited considerable seasonal and spatial variations with influence on the bacterial population. coral reef areas recorded higher bacterial population d ... | 2008 | 18831378 |
| chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxb) in rhizobacteria isolated from crop plants. | twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16s rrna analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the beta and gamma subclasses of proteobacteria and actinobacteria. among these, five genera, including dyella, burkholderia, alcaligenes, microbacterium and leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. the thiosulfate-oxidizing dyella, burkholderia, alcaligenes, microbacterium, lei ... | 2008 | 18832027 |
| siderophore-producing alcaligenes feacalis exhibited more biocontrol potential vis-à-vis chemical fungicide. | we report the in vitro phytopathogen suppression activity of siderophoregenic preparations of alcaligenes feacalis vis-à-vis the oraganochlorine fungicide, bavistin. siderophore-rich culture broth, siderophore-rich supernatant, and purified siderophore preparation exerted antifungal activity against aspergillus niger ncim 1025, a. flavus ncim 650, fusarium oxysporum ncim 1008, and alternaria alternata iari 715. among all the preparations, siderophore-rich broth exhibited potent antifungal activi ... | 2009 | 18836770 |
| antibiograms of pathogenic bacteria isolated from laboratory rabbits in ibadan, nigeria. | antibiotic sensitivity patterns of 21 bacterial isolates from some clinically ill new zealand rabbits were evaluated against 12 commonly used antibiotics by the kirby-bauer disk-diffusion sensitivity testing method. the 21 isolates consisted of six bordetella bronchiseptica, eight pasteurella multocida, four staphylococcus aureus and three pseudomonas alcaligenes that were associated with snuffles, pneumonia, otitis media, genital infections and conjunctivitis in these groups of caged rabbits. t ... | 2008 | 18840619 |
| [the spectrum of microbiological agents causing pulmonary malt-type lymphomas. a 16s rrna-based analysis of microbial diversity]. | for several anatomical localisations of extranodal marginal zone b-cell lymphoma of malt type (emzbcl), an association with chronic inflammation caused by microbiological agents (e.g. helicobacter pylori in the stomach) has been described. in the lung, a link between lymphomagenesis and a defined causative organism is still missing. a comprehensive diversity survey using 16s-rdna library construction followed by restriction fragment length polymorphism (rflp) analysis, sequencing, and phylogenet ... | 2008 | 18854998 |
| fate of a genetically modified bacterium in foregut of glassy-winged sharpshooter (hemiptera: cicadellidae). | symbiotic control is a new strategy being investigated to prevent the spread of insect-transmitted pathogens by reducing vector competence. we are developing this strategy to reduce the spread of xylella fastidiosa by homalodisca vitripennis (germar) [formerly homalodisca coagulata (say)] (hemiptera: cicadellidae), the glassy-winged sharpshooter. in this study, the fate of a transformed symbiotic bacterium, alcaligenes xylosoxidans variety denitriicans (s1axd), in the foregut of glassy-winged sh ... | 2008 | 18950032 |
| [methodological aspects of assessing chitin utilization by soil microorganisms]. | a computational method for estimating specific activity of chitin decomposition by microorganisms is proposed. spectrophotometric and gas chromatographic methods have been used to determine the rates of chitinase production, biomass accumulation, and carbon dioxide emission by pure cultures of microorganisms grown on a chitin-containing medium. among dominants of the chitinolytic community of chernozem (trichoderma viride, stretomyces albolongus, alcaligenes, and arthrobacter), the highest chiti ... | 2008 | 18956744 |
| biotechnological process for production of beta-dipeptides from cyanophycin on a technical scale and its optimization. | a triphasic process was developed for the production of beta dipeptides from cyanophycin (cgp) on a large scale. phase i comprises an optimized acid extraction method for technical isolation of cgp from biomass. it yielded highly purified cgp consisting of aspartate, arginine, and a little lysine. phase ii comprises the fermentative production of an extracellular cgpase (cphe(al)) from pseudomonas alcaligenes strain dip1 on a 500-liter scale in mineral salts medium, with citrate as the sole carb ... | 2009 | 18978083 |
| bacterial contamination in drinking water: a case study in rural areas of northern rajasthan, india. | the objective of this study was to determine the bacterial contaminations in drinking water samples collected form some rural habitations of northern rajasthan, india. a total of ten bacterial species: escherichia coli, pseudomonas aeruginosa, enterobacter aerogenes, klebsiella sp, proteus vulgaris, alcaligenes faecalis, bacillus cereus, staphylococcus aureus, streptococcus lactis and micrococcus luteum were identified form drinking water samples. the bacteria belonging to the family enterobacte ... | 2009 | 19023670 |
| escravos light crude oil degrading potentials of axenic and mixed bacterial cultures. | we assessed the relationship between growth profile and the extent of biodegradation of escravos light crude oil by axenic and mixed bacteria cultures in a shake flask. eleven petroleum-degrading bacteria were isolated by enrichment from oil-contaminated soils including, pseudomonas effusa, pseudomonas fluorescens, pseudomonas cruciviae, arthrobacter tumescens, pseudomonas species, pseudomonas tralucida, alcaligenes metacaligenes, micrococcus colpogenes, bacillus badius, nocardia paraffinae and ... | 2008 | 19029769 |
| identification and genetic characterization of phenol-degrading bacteria from leaf microbial communities. | microbial communities on aerial plant leaves may contribute to the degradation of organic air pollutants such as phenol. epiphytic bacteria capable of phenol degradation were isolated from the leaves of green ash trees grown at a site rich in airborne pollutants. bacteria from these communities were subjected, in parallel, to serial enrichments with increasing concentrations of phenol and to direct plating followed by a colony autoradiography screen in the presence of radiolabeled phenol. ten is ... | 2009 | 19034559 |
| bacterial community and biological nitrate removal: comparisons of autotrophic and heterotrophic reactors for denitrification with raw sewage. | an autotrophic denitrification reactor (adr-1) and a heterotrophic denitrification reactor (hdr-2) were operated to remove nitrate and nitrite in an anoxic environment in raw sewage. the no3-n removal rate of adr-1 was shown to range from 52.8% to 78.7%, which was higher than the no3-n removal rate of hdr-2. specific denitrification rates (sdnr) of adr-1 and hdr-2 were 3.0 to 4.0 and 1.1 to 1.2mgno3- n/gvss/h, respectively. from results of restriction fragment length polymorphism (rflp) of the 1 ... | 2008 | 19047828 |
| alcaligenes xylosoxidans endophthalmitis following phacoemulsification and intraocular lens implantation. | five consecutive cases of endophthalmitis that developed after cataract extraction by a single surgeon using the same operating room during one morning session are described. following preoperative topical administration of ciprofloxacin, surgery consisted of phacoemulsification with peristaltic pump and fluid venting, polymethylmethacrylate intraocular lens implantation, and corneal suture. no complications occurred during surgery. all five patients developed endophthalmitis caused by infection ... | 2008 | 19065983 |
| [polyhydroxyalkanoates microbiological synthesis from food wastes]. | to reduce the production cost of polyhydroxyalkanoates (phas), the process feasibility and physicochemical properties of phas synthesized by alcaligenes latus, staphylococcus epidermidis and activated sludge from malt waste, soy waste, confectionary waste, ice cream waste, milk waste, sesame oil and vinegar waste were analyzed. results showed that through two-stage fed-batch fermentation, the maximum yield of phas accumulated by the three kinds of microorganisms from malt waste was 70.1%, 16.0% ... | 2008 | 19068658 |
| isolation and identification of atrazine-degrading bacteria from corn field soil in fars province of iran. | in this study several agricultural fields with a long history of atrazine application in fars province of iran have been explored for their potential of atrazine biodegradation. after several subculturing for a period of 300 days acclimation, leads to an enhancement of atrazine biodegradation rate. a successful enrichment culture with a high capability for atrazine degradation was obtained (88%). a combination of enrichment culture technique, in a basal salt medium containing atrazine and carbon ... | 2007 | 19069990 |
| conformational exchange in pseudoazurin: different kinds of microsecond to millisecond dynamics characterized by their ph and buffer dependence using 15n nmr relaxation. | the dynamics of the reduced form of the blue copper protein pseudoazurin from alcaligenes faecalis s-6 was investigated using (15)n relaxation measurements with a focus on the dynamics of the micro- to millisecond time scale. different types of conformational exchange processes are observed in the protein on this time scale. at low ph, the protonation of the c-terminal copper-ligated histidine, his81, is observed. a comparison of the exchange rates in the presence and absence of added buffers sh ... | 2009 | 19072172 |
| accumulation of intracellular polyhydroxybutyrate in alcaligenes sp. d(2) under phenol stress. | alcaligenes sp. d(2) isolated from soil was earlier reported as a potent phenol-degrading organism. in the fourier transform/infrared spectroscopic analysis of the biodegraded sample, the aromatic stretching was missing and the spectrum gave evidence for the presence of polyhydroxybutyric acid along with its depolymerized products. in the gas chromatogram of the biodegraded sample, the peak of phenol at 14.997 min was absent and there were many peaks after 20 min. the organism could carry out 10 ... | 2009 | 19082761 |
| simultaneous determination of glucose and choline based on the intrinsic fluorescence of the enzymes. | it has been possible to perform the simultaneous determination of choline and glucose using the intrinsic fluorescence of the corresponding enzyme as an analytical signal. this can be done in two ways. first, for low glucose and choline concentrations (about 0.55 mm and 0.75 microm respectively) two differentiated signals, without mutual interference, are obtained for both analytes in the same measurement. second, when glucose and choline concentrations are higher, a new model has been designed ... | 2009 | 19089602 |
| alcaligenes xylosoxidans cholecystitis and meningitis acquired during bathing procedures in a burn unit: a case report. | the information in this article was presented at the 37th annual meeting of the japan society of burn, nagoya, japan, june 7-8, 2008. alcaligenes xylosoxidans, a nonfermentative, gram-negative rod often found in aqueous environments, has been isolated from respirators, incubators, and disinfectant solutions in the hospital environment. it is known to cause disease in immunocompromised (eg, burn) patients and represents a cross-contamination risk related to wound care. in the authors' burn unit, ... | 2008 | 19104123 |
| fate of chlortetracycline- and tylosin-resistant bacteria in an aerobic thermophilic sequencing batch reactor treating swine waste. | antibiotics have been added to animal feed for decades. consequently, food animals and their wastes constitute a reservoir of antibiotic-resistant bacteria. the objective of this work was to characterize the impact of an aerobic thermophilic biotreatment on aerobic, antibiotic-resistant bacteria in swine waste. the proportion of tylosin- and chlortetracycline-resistant bacteria grown at 25 degrees c, 37 degrees c, and 60 degrees c decreased after treatment, but they were still abundant (10(2) to ... | 2009 | 19125305 |
| atrazine degradation by stable mixed cultures enriched from agricultural soil and their characterization. | the aim of this work was to enrich stable mixed cultures from atrazine-contaminated soil. the cultures were examined for their atrazine biodegradation efficiencies in comparison with j14a, a known atrazine-degrading strain of agrobacterium radiobacter. the cultures were also characterized to identify community structure and bacterial species present. | 2009 | 19191954 |
| crystallization and preliminary x-ray diffraction analysis of a complex between the electron-transfer partners hexameric cu-containing nitrite reductase and pseudoazurin. | the complex between cu-containing nitrite reductase (hdnir) and its electron-donor protein pseudoazurin (hdpaz) from hyphomicrobium denitrificans has been crystallized. the crystals were obtained from a mixture of the two proteins using the hanging-drop vapour-diffusion method in the presence of polyethylene glycol (peg) and 2-methyl-2,4-pentanediol (mpd) as precipitants. sds-page analysis demonstrated that the crystals contained both proteins. the x-ray diffraction experiment was carried out at ... | 2009 | 19193999 |
| purification and characterization of arsenite oxidase from arthrobacter sp. | the chemolithoautotroph, arthrobacter sp.15b oxidizes arsenite to arsenate using a membrane bound arsenite oxidase. the enzyme arsenite oxidase is purified to its homogeneity and identified using maldi-tof ms analysis. upon further characterization, it was observed that the enzyme is a heterodimer showing native molecular mass as approximately 100 kda and appeared as two subunits of approximately 85 kda lsu and 14 kda ssu on sds-page. the v(max) and k(m) values of the enzyme was found to be 2.45 ... | 2009 | 19214757 |
| nickel(ii)-substituted azurin i from alcaligenes xylosoxidans as characterized by resonance raman spectroscopy at cryogenic temperature. | metal-substituted blue copper proteins (cupredoxins) have been successfully used to study the effect of metal-ion identity on their active-site properties, specifically the coordination geometry and metal-ligand bond strengths. in this work, low-temperature (77 k) resonance raman (rr) spectra of the blue copper protein alcaligenes xylosoxidans azurin i and its ni(ii) derivative are reported. a detailed analysis of all observed bands is presented and responsiveness to metal substitution is discus ... | 2009 | 19224256 |
| identification and characterization of sulfur-oxidizing bacteria in an artificial wetland that treats wastewater from a tannery. | wastewater from tanneries contains high concentrations of organic matter, chromium, nitrogen, and sulfur compounds. in this study, an artificial wetland is is used as the tertiary treatment in a tannery in león gto., méxico. it consists of three subplots with an area of about 450 m2. two subplots were planted with typha sp. and the third with scirpus americanus. geochemical analyses along the flowpath of the wetland show that contaminants were effectively attenuated. the most probable number tec ... | 2008 | 19260219 |
| characterization of a polycyclic aromatic hydrocarbon degradation gene cluster in a phenanthrene-degrading acidovorax strain. | acidovorax sp. strain na3 was isolated from polycyclic aromatic hydrocarbon (pah)-contaminated soil that had been treated in a bioreactor and enriched with phenanthrene. the 16s rrna gene of the isolate possessed 99.8 to 99.9% similarity to the dominant sequences recovered during a previous stable-isotope probing experiment with [u-(13)c]phenanthrene on the same soil (d. r. singleton, s. n. powell, r. sangaiah, a. gold, l. m. ball, and m. d. aitken, appl. environ. microbiol. 71:1202-1209, 2005). ... | 2009 | 19270134 |
| a tn5051-like mer-containing transposon identified in a heavy metal tolerant strain achromobacter sp. ao22. | achromobacter sp. ao22 (formerly alcaligenes sp. ao22), a bacterial strain isolated from a lead-contaminated industrial site in australia, was previously found to be resistant to moderate to high levels of mercury, copper and other heavy metals. however, the nature and location of the genetic basis for mercuric ion resistance in this strain, had not been previously identified. | 2009 | 19284535 |
| investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from south china sea sponges. | to obtain bacteria with pks (polyketide synthase) genes and antimicrobial activity from sponges. | 2009 | 19302490 |
| effects of organoclays on soil eubacterial community assessed by molecular approaches. | the aim of this study was to evaluate the effects of the commercial organoclays, cloisite 30b, nanofil 804 and dellite 26c on soil eubacterial community. an enrichment test was carried out on nutrient broth containing the organoclay and the microorganisms previously isolated from soil. four transfers were made, each after 7 days incubation. the molecular analyses on the eubacterial community were performed before treatment and 7 days after each transfer. dna was extracted, amplified with eubacte ... | 2009 | 19304378 |
| screening of strain-specific actinobacillus pleuropneumoniae genes using a combination method. | we describe a three-step method designed to identify distinct antigen-coding genes between two related bacterial genomes by: (a) constructing a subtractive library using representational difference analysis (rda), (b) characterization of gene expression in vitro using a ribosome display system combined with antibody screening and (c) gene recovery and confirmation using rt-pcr and reverse southern hybridization, respectively. to test the efficacy of this strategy we screened the antigen-coding g ... | 2009 | 19318057 |
| microbiological spectrum of organisms isolated from explanted scleral buckles. | scleral buckle removal is an uncommon procedure performed for various reasons. microbiological information on explanted scleral buckles remains limited. the authors identified 37 cases of scleral buckle removal during an 18-year period. bacterial cultures isolated an organism in 4 of 9 patients (44%) with clinical infection. organisms identified included methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa, mycobacterium abscessus, and coagulase-negative staphylococcus species. ba ... | 2009 | 19320315 |
| the mechanism of domain alternation in the acyl-adenylate forming ligase superfamily member 4-chlorobenzoate: coenzyme a ligase. | 4-chlorobenzoate:coa ligase (cbl) belongs to the adenylate-forming family of enzymes that catalyze a two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme a or an acyl-carrier protein. the active site is located at the interface of a large n-terminal domain and a smaller c-terminal domain. crystallographic structures have been determined at multiple steps along the reaction pathway and form the ba ... | 2009 | 19320426 |
| arsenite oxidation by immobilized cells of alcaligenes faecalis strain o1201 in a fluidized-bed reactor. | arsenic(iii) oxidation was evaluated in a continuous-flow fluidized-bed reactor (fbr) with alcaligenes faecalis strain 01201 immobilized in gel beads. the fbr was operated under 300 mg/l citrate and a range of influent as(iii) concentrations (75 to 3000 mg/l) at short hydraulic retention times (1.06 to 3.17 hours). the ph and temperature in the fbr were maintained at optimal growth conditions for strain o1201 (ph 7 and 30 degrees c) throughout the study. a total of 10 quasi-steady-state operatin ... | 2009 | 19323288 |
| bioproduction of glycolic acid from glycolonitrile with a new bacterial isolate of alcaligenes sp. ecu0401. | alcaligenes sp. ecu0401 has been isolated from soil samples with high nitrilase activity against glycolonitrile using the enrichment culture technique. the preferred carbon/nitrogen sources and metal ions were sodium acetate, a composite of peptone and yeast extract, and cu(2+), respectively. glycolic acid was obtained in a yield of 96.5% after 14 h of biotransformation from a total of 200 mm glycolonitrile in the mode of sequential addition during the cultivation of alcaligenes sp. ecu0401 in a ... | 2010 | 19333557 |
| activation parameters for heme-no binding in alcaligenes xylosoxidans cytochrome c': the putative dinitrosyl intermediate forms via a dissociative mechanism. | the bacterial heme protein alcaligenes xylosoxidans cytochrome c' (axcp) forms a novel five-coordinate heme-nitrosyl (5c-no) complex in which no resides at the proximal heme face in place of the endogenous protein ligand. intriguingly, axcp shares no-binding properties with the eukaryotic no-sensor, soluble guanylate cyclase (sgc), including 5c-no formation via two no-dependent reactions. for both proteins, a model has been proposed in which no binds to the vacant distal face to form a transient ... | 2009 | 19334778 |
| structure of d-3-hydroxybutyrate dehydrogenase prepared in the presence of the substrate d-3-hydroxybutyrate and nad+. | d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis catalyzes the reversible conversion between d-3-hydroxybutyrate and acetoacetate. the enzyme was crystallized in the presence of the substrate d-3-hydroxybutyrate and the cofactor nad(+) at the optimum ph for the catalytic reaction. the structure, which was solved by x-ray crystallography, is isomorphous to that of the complex with the substrate analogue acetate. the product as well as the substrate molecule are accommodated well in the ... | 2009 | 19342772 |
| [denitrifying bacteria isolated from river sediment and its nitrogen and phosphorus removal capacity from river water]. | 20 strains of bacteria were isolated from river sediment using enrichment culture medium for denitrification, and the denitrification intensity was determined. f10, one of bacteria strain, was identified having the highest denitrifying intensity, and further used to test its role in the removing of nitrogen and phosphorus from wastewater in laboratory. by checking the individual morphology, colony culture characteristics, dna sequencing and 16s rdna gene bank, f10 was identified as alcaligenes f ... | 2009 | 19353863 |
| structures of various cytochromes c evaluated from the redox behaviors using the optically active co(iii) complex-modified au electrode. | electrochemical studies of three c-type cytochromes (cyt c from horse heart, cyt c(2) from rhodospirillum rubrum, and cyt c(553) from alcaligenes xylosoxidans gifu 1051) were performed by using the optically active co(iii) complex-modified au electrode. three cytochromes gave different redox behaviors reflected from the respective structural information. from relationship between their redox behaviors and their structural characteristics, we evaluated the solution structure around heme center of ... | 2007 | 19356022 |
| structure, assembly, and mechanism of a plp-dependent dodecameric l-aspartate beta-decarboxylase. | the type-i plp enzyme l-aspartate beta-decarboxylase converts aspartate to alanine and co(2). similar to the homodimeric aminotransferases, its protein subunit comprises a large and a small domain, of 410 and 120 residues, respectively. the crystal structure reveals a dodecamer made of six identical dimers arranged in a truncated tetrahedron whose assembly involves tetramer and hexamer as intermediates. the additional helical motifs i and ii participate in the oligomer formation. triple mutation ... | 2009 | 19368885 |
| pseudomonas aeruginosa las quorum sensing autoinducer suppresses growth and biofilm production in legionella species. | bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (qs). recent studies have shown that the las qs autoinducer n-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-c(12)-hsl) of pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. in this study, we report the effects of pseudomonas 3-oxo-c(12)-hsl on the growth and suppression of virulence factors ... | 2009 | 19383702 |
| effect of periplasmic nitrate reductase on diauxic lag of paracoccus pantotrophus. | paracoccus pantotrophus expresses two nitrate reductases-membrane bound nitrate reductase (nar) and periplasmic nitrate reductase (nap). in growth experiments with two denitrifying species (paracoccus pantotrophus and alcaligenes eutrophus) that have both nap and nar and two species (pseudomonas denitrificans and pseudomonas fluorescens) with nar only, it was found that diauxic lag is shorter for bacteria that express nap. in p. pantotrophus, napedabc encodes the periplasmic nitrate reductase. t ... | 2009 | 19399903 |
| impact of biocide treatments on the bacterial communities of the lascaux cave. | the lascaux cave contains a remarkable set of paintings from the upper palaeolithic. shortly after discovery in 1940, the cave was modified for public viewing and, in 2001, was invaded by a fusarium solani species complex. benzalkonium chloride was used from 2001 to 2004 to eliminate the fungal outbreak. in this study, we carried out a sampling in most of the cave halls and galleries. sequence analysis and isolation methods detected that the most abundant genera of bacteria were ralstonia and ps ... | 2009 | 19404600 |
| unusual microbes in asthma exacerbation: alcaligenes xylosoxidans and leishmania. | asthma is a chronic inflammatory condition characterised by a variable degree of airflow limitation. exacerbations during the course of asthma often occur due to environmental factors or infectious, mostly viral, aetiology. the present study reports the case of a 61-yr-old male with severe asthma hospitalised due to increasing respiratory distress. since recovery was delayed despite anti-obstructive/anti-inflammatory and antibiotic therapy, further diagnostic procedures, including bronchoscopy, ... | 2009 | 19407055 |
| linear alkylbenzene sulfonate tolerance in bacteria isolated from sediment of tropical water bodies polluted with detergents. | the discharge of untreated detergent-bearing waste introduces linear alklcylbenzene sulfonates (las) to the aquatic environment. the surfactant persists in some streams and rivers in nigeria, some is adsorbed to suspended materials and end in the sediment of the receiving water bodies. in this study, bacteria isolated from sediments of some tropical detergent-effluent-polluted streams were tested for tolerance to las using the media dilution technique. las-tolerance was indicated by growth of th ... | 2008 | 19419067 |
| strain-specific virulence of bordetella hinzii in poultry. | bordetella hinzii is commonly acquired from the respiratory tract of diseased poultry but is generally regarded as nonpathogenic in avian hosts because attempts to demonstrate disease following experimental infection of chickens and turkeys have failed. recently, with the availability of highly specific dna-based methods for identification of this agent, it was recognized that some isolates used in previous studies were misidentified at the time of their acquisition as bordetella avium, b. avium ... | 2009 | 19432003 |
| [cellulose-utilising bacterial association]. | | 2009 | 19449742 |
| impact of antifungals producing rhizobacteria on the performance of vigna radiata in the presence of arbuscular mycorrhizal fungi. | plant growth-promoting rhizobacteria (pgpr) that produce antifungal metabolites are potential threats for the arbuscular mycorrhizal (am) fungi known for their beneficial symbiosis with plants that is crucially important for low-input sustainable agriculture. to address this issue, we used a compartmented container system where test plants, vigna radiata, could only reach a separate nutrient-rich compartment indirectly via the hyphae of am fungi associated with their roots. in this system, where ... | 2009 | 19458967 |
| alcaligenes xylosoxidans keratitis after autologous cultivated limbal epithelium transplant. | | 2009 | 19491995 |
| respiratory microbiology of patients with cystic fibrosis in the united states, 1995 to 2005. | numerous improvements in diagnostic and therapeutic strategies for patients with cystic fibrosis (cf) have occurred during the past 2 decades. we hypothesized that these changes could impact trends in respiratory microbiology. | 2009 | 19505987 |
| multiple drug-resistant alcaligenes xylosoxidans keratitis in a sanitation worker. | to report a case of daily wear soft contact lens-associated keratitis caused by alcaligenes xylosoxidans in a portable toilet sanitation worker. | 2009 | 19516143 |
| efficacy of bacterial consortium-aie2 for contemporaneous cr(vi) and azo dye bioremediation in batch and continuous bioreactor systems, monitoring steady-state bacterial dynamics using qpcr assays. | bacterial consortium-aie2 with a capability of contemporaneous cr(vi) reduction and azo dye rv5 decolourization was developed from industrial wastewaters by enrichment culture technique. the 16s rrna gene based molecular analyses revealed that the consortium bacterial community structure consisted of four bacterial strains namely, alcaligenes sp. dma, bacillus sp. dmb, stenotrophomonas sp. dms and enterococcus sp. dme. cumulative mechanism of cr(vi) reduction by the consortium was determined usi ... | 2009 | 19517254 |
| [evaluation of occurrence of alcaligenes faecalis in clinical samples of patients of the university hospital in bydgoszcz]. | alcaligenes faecalis is an aerobic gram-negative, non-fermentative rod. it's saprophyte of water and soil. it may be recovered from wet places of hospital environment. it is considered as an opportunistic pathogen. the aim of this review was evaluation of occurrence in clinical samples and susceptibility to antibiotics of 72 a. faecalis strains isolated in years 2003-2008. over 30% of strains were isolated from patients in surgical ward, 19.6% from patients in outpatient clinic and almost 14% fr ... | 2009 | 19517818 |
| [etiology of infections in the wounded victims of wenchuan earthquake]. | to analyze the etiology of infections in the wounded victims of wenchuan earthquake. | 2009 | 19567109 |
| identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid psah in alcaligenes sp. strain o-1. | alcaligenes sp. strain o-1 inducibly deaminates 2-aminobenzenesulfonate (abs) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. this intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (scm). the complete pathway is encoded on the 180-kb plasmid psah, 20kb of which was sequenced. twenty open reading frames (orfs) were detected. two clusters (abs and scm) with degradative genes were surroun ... | 2010 | 19577910 |
| identification of amino acid residues responsible for the enantioselectivity and amide formation capacity of the arylacetonitrilase from pseudomonas fluorescens ebc191. | the nitrilase from pseudomonas fluorescens ebc191 converted (r,s)-mandelonitrile with a low enantioselectivity to (r)-mandelic acid and (s)-mandeloamide in a ratio of about 4:1. in contrast, the same substrate was hydrolyzed by the homologous nitrilase from alcaligenes faecalis atcc 8750 almost exclusively to (r)-mandelic acid. a chimeric enzyme between both nitrilases was constructed, which represented in total 16 amino acid exchanges in the central part of the nitrilase from p. fluorescens ebc ... | 2009 | 19581475 |
| demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases. | the reduction of nitrite (no2-) into nitric oxide (no), catalyzed by nitrite reductase, is an important reaction in the denitrification pathway. in this study, the catalytic mechanism of the copper-containing nitrite reductase from alcaligenes xylosoxidans (axnir) has been studied using single and multiple turnover experiments at ph 7.0 and is shown to involve two protons. a novel steady-state assay was developed, in which deoxyhemoglobin was employed as an no scavenger. a moderate solvent kinet ... | 2009 | 19586913 |
| complicated intra-abdominal infection caused by extended drug-resistant achromobacter xylosoxidans. | achromobacter xylosoxidans (formerly alcaligenes xylosoxidans) is a rare but important nosocomial pathogen. antibiotic resistance has been increasing during the past decade. a. xylosoxidans may be confused with pseudomonas spp. but, unlike pseudomonas spp., this organism has peritrichous flagella. complicated intra-abdominal infection with a. xylosoxidans has rarely been reported in the literature. this report is of an immunocompetent patient with acute cholecystitis complicated by an intra-abdo ... | 2009 | 19597652 |
| biodegradation of complex bacteria on phenolic derivatives in river water. | to isolate, incubate, and identify 4-chlorophenol-degrading complex bacteria, determine the tolerance of these bacteria to phenolic derivatives and study their synergetic metabolism as well as the aboriginal micrpbes and co-metabolic degradation of mixed chlorophenols in river water. | 2009 | 19623744 |
| [production of biodemulsifier by alcaligenes sp. xj-t-1 with waste frying oil]. | as a new member of demulsifier family, biodemulsifier is applied in oil-water emulsion breaking. a strain, xj-t-1, was isolated from petroleum-contaminated soil and identified as alcaligenes sp.. its physicochemical properties, demulsification capability and utilization of waste oil were further investigated. with waste frying oil (wfo) as carbon source, the demulsifier produced by alcaligenes sp. showed high demulsifying capability. moreover, the production of demulsifier was 4.6 times of that ... | 2009 | 19662868 |
| decreasing ammonium generation using hydrogenotrophic bacteria in the process of nitrate reduction by nanoscale zero-valent iron. | an integrated nitrate treatment using nanoscale zero-valent iron (nzvi) and alcaligenes eutrophus, which is a kind of hydrogenotrophic denitrifying bacteria, was conducted to remove nitrate and decrease ammonium generation. within 8 days, nitrate was removed completely in the reactors containing nzvi particles plus bacteria while the proportion of ammonium generated was only 33%. that is a lower reduction rate but a smaller proportion of ammonium relative to that in abiotic reactors. it was also ... | 2009 | 19665759 |
| characterization of sucrose-glutamic acid maillard products (sgmps) degrading bacteria and their metabolites. | two aerobic bacteria rnbs1 and rnbs3 capable to degrade and utilize sucrose-glutamic acid maillard products (sgmps) as carbon, nitrogen and energy source were isolated and characterized as alcaligenes faecalis (dq659619) and bacillus cereus (dq659620) respectively by 16s rrna gene sequence analysis. in present study, mixed bacterial culture was found more effective compared to axenic culture rnbs1 and rnbs3 decolourizing 73.79%, 66.80% and 62.56% sgmps, respectively. the sgmps catabolizing enzym ... | 2009 | 19665891 |
| paenalcaligenes hominis gen. nov., sp. nov., a new member of the family alcaligenaceae. | a beige-pigmented bacterium (strain ccug 53761a(t)) was isolated from human blood from an 85-year-old man in göteborg, sweden. comparative analysis of 16s rrna gene sequences showed that this bacterium displayed <95 % similarity to all described species of the genera of the family alcaligenaceae. it grouped within the radiation of the genus alcaligenes, but showed only 93.0-94.8 % similarity to type strains of members of this genus (alcaligenes faecalis subsp. parafaecalis, 94.8 %; alcaligenes f ... | 2010 | 19684310 |
| molecular and kinetic characterization of mixed cultures degrading natural and synthetic estrogens. | the biodegradation of estradiol (e2), estrone (e1), and ethinylestradiol (ee2) was investigated using mixed bacterial cultures enriched from activated sludge. enrichments were carried out on e2 or ee2 in batch conditions with acetonitrile as additional carbon source. degradation experiments were performed both using hormones as sole carbon source or with an additional source. the hormones were completely degraded by these cultures. estradiol was rapidly converted to e1 within 24 h. thereafter, e ... | 2010 | 19685239 |
| biphenyl-metabolizing bacteria in the rhizosphere of horseradish and bulk soil contaminated by polychlorinated biphenyls as revealed by stable isotope probing. | dna-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used in order to identify members of the microbial community that metabolize biphenyl in the rhizosphere of horseradish (armoracia rusticana) cultivated in soil contaminated with polychlorinated biphenyls (pcbs) compared to members of the microbial community in initial, uncultivated bulk soil. on the basis of early and recurrent detection of their 16s rrna genes in clone libraries construct ... | 2009 | 19700551 |
| bacterial growth and biofilm production on pyrene. | enrichment cultures inoculated with arctic soil yielded a biofilm that grew on pyrene and phenanthrene. in a 60-day period, the biofilm degraded 20 microg ml(-1) pyrene or 39 microg ml(-1) phenanthrene. single colonized pyrene crystals (approximately 1.5x0.75x0.35 mm) yielded 10(11) culturable heterotrophs and 10(5) biofilm propagules. analysis of ribosomal intergenic spacers identified six phylotypes in a clone library from the pyrene biofilm. analysis of 16s rrna gene sequences indicated that ... | 2002 | 19709207 |
| microbial community composition at an ethane pyrolysis plant site at different hydrocarbon inputs. | aerobic biodegradation of c5+, a contaminant at ethane pyrolysis plants, consisting mainly of benzene, toluene, xylene, styrene, dicyclopentadiene (dcpd) and naphthalene, removes all components except the recalcitrant dcpd. the number (n) of total culturable heterotrophs in contaminated and uncontaminated soil cultures increased up to three orders of magnitude upon incubation with c5+, or with c5+ components other than dcpd. exposure of soil cultures to c5+ for 2-4 weeks and to dcpd for a furthe ... | 2002 | 19709231 |
| molecular characterization of microbial community in nitrate-removing activated sludge. | abstract the microbial community composition and dominant denitrifying populations in high-nitrate-removing (cr-i) and low-nitrate-removing (cr-ii) activated sludge from continuous bioreactors were investigated with most probable number (mpn) enumeration, fluorescence in situ hybridization (fish) and 16s rdna characterization. mpns of nitrate-reducing bacteria of sludge cr-i and sludge cr-ii were 2.82x10(7) and 2.69x10(4) colony-forming units ml(-1), respectively. eight denitrifying bacteria and ... | 2002 | 19709242 |
| nitrite reductase genes in halobenzoate degrading denitrifying bacteria. | abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ... | 2003 | 19719666 |
| eiseniicola composti gen. nov., sp. nov., with antifungal activity against plant pathogenic fungi. | a gram-negative, short rod-shaped bacterial strain, yc06271t, was isolated from the vermicompost (vc) collected at masan, korea and its taxonomic position was investigated by a polyphasic taxonomic approach. strain yc06271t grew optimally at 28-30 degrees c and at ph 7.0-9.0. the 16s rrna gene sequence of strain yc06271t was most closely related to members of the genera bordetella (96.4-95.8 %), achromobacter (96.0-95.7 %), alcaligenes (96.0-94.2 %), pusillimonas noertemannii (95.9 %), pigmentip ... | 2010 | 19734285 |
| [epidemiological survey of bacteria isolated from the respiratory tract of cystic fibrosis patients]. | with the aim of characterizing the evolution of the epidemiological profile of respiratory bacterial infections of patients having cystic fibrosis (cf), the authors conducted a retrospective analysis about it's incidence and prevalence in 78 cf patients followed at the cf specialized centre, paediatric department, santa maria hospital, lisbon, during a 5 years period (1995-1999). pseudomonas aeruginosa was the most frequently isolated bacteria during the first three years of the study (60-73%), ... | 2003 | 19771691 |
| characteristics and function of alcaligenes sp. nbrc 14130 esterase catalysing the stereo-selective hydrolysis of ethyl chrysanthemate. | alcaligenes sp. nbrc 14130 was found as a strain hydrolysing a mixture of (+/-)-trans- and (+/-)-cis ethyl chrysanthemates to (1r,3r)-(+)-trans-chrysanthemic acid. the alcaligenes cells also have hydrolytic activity for 6-aminohexanoate-cyclic dimer (6-ahcd, 1,8-diazacyclotetradecane-2,9-dione). the correlation of function on the enzyme from the alcaligenes strain with hydrolysis activities for both ethyl chrysanthemate and 6-ahcd was demonstrated. | 2010 | 19778353 |
| kinetics and metabolic versatility of highly tolerant phenol degrading alcaligenes strain tw1. | a bacterium that could completely metabolize phenol in batch culture supplied with up to 1200 mg phenol l(-1) at room temperature (25 degrees c) was isolated from the activated sludge of the industrial wastewater treatment plant of a coke company (cairo, egypt). morphological and physiological characterization showed strain tw1 was a motile, strictly aerobic, gram negative and short-rod occurring singly or in clusters. partial 16s rrna gene sequence analysis revealed strain tw1 belonged to the b ... | 2010 | 19783362 |
| incidence, clinical features, causative organisms, and visual outcomes of delayed-onset pseudophakic endophthalmitis. | to report the incidence, clinical features, causative organisms, and visual outcomes associated with delayed-onset pseudophakic endophthalmitis. | 2009 | 19787601 |
| [alcaligenes xylosoxidans bacteremia in a patient with acute lymphoblastic leukemia]. | alcaligenes xylosoxidans which is an aerobic, non-fermentative gram-negative bacillus found in aqueous environments and human flora, can lead to opportunistic infections. it causes infections in elderly, immunocompromised patients, patients with chronic disorders or premature infants. in this report, a case of a. xylosoxidans bacteremia that developed in a child with acute lymphoblastic leukemia (all) was presented. four-years-old male patient under all induction therapy was admitted with sympto ... | 2009 | 19795625 |
| purification and characterization of a cis-epoxysuccinic acid hydrolase from bordetella sp. strain 1-3. | purification of a cis-epoxysuccinic acid hydrolase was achieved by ammonium sulfate precipitation, ionic exchange chromatography, hydrophobic interaction chromatography followed by size-exclusion chromatography. the enzyme was purified 177-fold with a yield of 14.4%. the apparent molecular mass of the enzyme was determined to be 33kda under denaturing conditions. the optimum ph for enzyme activity was 7.0, and the enzyme exhibited maximum activity at about 45 degrees c in 50mm sodium phosphate b ... | 2010 | 19800407 |
| the susceptibility of nosocomial pathogens to ceftazidime. | the activities in vitro of ceftazidime and of cefuroxime have been compared against 201 strains of bacteria which had been isolated either from outbreaks of nosocomial infection or were typical of strains causing such outbreaks. ceftazidime was found to be about 1000 times more active against pseudomonas spp. and proteus vulgaris, and between 10 and 100 times more active against klebsiella pneumoniae, enterobacter spp., citrobacter freundii, serratia marcescens, proteus rettgeri, proteus morgani ... | 1981 | 19802966 |
| in vitro pharmacodynamics of levofloxacin and other aerosolized antibiotics under multiple conditions relevant to chronic pulmonary infection in cystic fibrosis. | the inhalational administration of antibiotics can provide high concentrations locally in the lungs of cystic fibrosis patients and, thus, can be useful for the treatment of chronic bacterial infections. the present study evaluated the in vitro activities of levofloxacin, ciprofloxacin, tobramycin, amikacin, and aztreonam against clinical isolates of pseudomonas aeruginosa, burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and staphylococcus aureus from cystic ... | 2010 | 19805554 |
| biosynthetic pathway of sugar nucleotides essential for welan gum production in alcaligenes sp. cgmcc2428. | welan gum is a microbial polysaccharide produced by alcaligenes sp. cgmcc2428 that has d-glucose, d-glucuronic acid, d-glucose, and l-rhamnose as the main structural unit. the biosynthetic pathway of sugar nucleotides essential for producing welan gum in this strain was established in the following ways: (1) the detection of the presence of several intermediates and key enzymes; (2) the analysis of the response upon addition of precursors to the culture medium; (3) the correlation of the activit ... | 2010 | 19838696 |
| immobilization of alcaligenes faecalis penicillin g acylase on epoxy-type supports. | alcaligenes faecalis penicillin g acylase has several desired features over other penicillin g acylases and its use in industry requires immobilization. in this work, two novel supports zh-ep (epoxy-type) and zh-ha (epoxy-amino type) were used to immobilize alcaligenes faecalis penicillin g acylase (afpga) with eupergit c as reference. the saturation of immobilized protein on zh-ep (269 mg/g, 116 h) and zh-ha (296 mg/g, 15 h) was obtained more rapidly than eupergit c (197 mg/g, 260 h). and the a ... | 2009 | 19845283 |
| comparative impact of cadmium on two phenanthrene-degrading bacteria isolated from cadmium and phenanthrene co-contaminated soil in china. | alcaligenes faecalis strain j08 and brevundimonas sp. strain x08 were isolated from soils co-contaminated by cadmium (cd) and polycyclic aromatic hydrocarbons (pahs) in northeast china. the two strains of bacteria were identified by phenotypic tests and 16s rdna. different cd treatments (0.01 mm, 0.1mm, 0.5mm) showed no significant influence (p>0.05) on the biodegradation of phenanthrene by a. faecalis strain j08. brevundimonas sp. strain x08 also presented no significant differences in the biod ... | 2010 | 19853994 |
| a case of endocarditis of difficult diagnosis in dialysis: could "pest" friends be involved? | we report a case of a. xylosoxidans endocarditis of larvate clinical presentation in a central venous catheter-dependent hemodialysis patient of difficult diagnosis and poor evolution despite a high index of suspicion and consequent assessment. a 50-year-old man on hemodialysis presented with inflammatory-malnutrition parameters during the months prior to diagnosis of endocarditis, whilst he was otherwise asymptomatic. no vegetations were detectable on his cardiac valves at repeated echocardiogr ... | 2009 | 19863886 |
| assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance. | to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health. | 2009 | 19880150 |
| effect of the earthworms lumbricus terrestris and aporrectodea caliginosa on bacterial diversity in soil. | earthworms ingest large amounts of soil and have the potential to radically alter the biomass, activity, and structure of the soil microbial community. in this study, the diversity of eight bacterial groups from fresh soil, gut, and casts of the earthworms lumbricus terrestris and aporrectodea caliginosa were studied by single-strand conformation polymorphism (sscp) analysis using both newly designed 16s rrna gene-specific primer sets targeting alphaproteobacteria, betaproteobacteria, gammaprote ... | 2010 | 19888626 |
| outbreak of alcaligenes faecalis pseudobacteraemia in neonatology and paediatric units. | | 2010 | 19900735 |
| protonation of type-1 cu bound histidines: a quantum chemical study. | the protonation of the solvent-exposed histidine ligands of the type-1 cu sites in five proteins, thiobacillus ferrooxidans rusticyanin, pseudomonas aeruginosa azurin, fern plastocyanin, alcaligenes faecalis pseudoazurin, and paracoccus versutus amicyanin, were studied with quantum chemical methods and conductorlike polarizable continuum model (cpcm). active site model molecules consisting of approximately 140 atoms were extracted from x-ray crystal structures and optimized with the homogeneous ... | 2010 | 20000723 |
| regioselective lipase-catalyzed synthesis of 3-o-acyl derivatives of resveratrol and study of their antioxidant properties. | one of the approaches to increasing the bioavailability of resveratrol is to protect its 3-oh phenolic group. in this work, regioselective acylation of resveratrol at 3-oh was achieved by transesterification with vinyl acetate catalyzed by immobilized lipase from alcaligenes sp. (lipase qlg). the maximum yield of 3-o-acetylresveratrol was approximately 75%, as the lipase also catalyzes its further acetylation affording the diester 3,4'-di-o-acetylresveratrol and finally the peracetylated derivat ... | 2010 | 20017485 |
| method to detect only viable cells in microbial ecology. | propidium monoazide can limit the analysis of microbial communities derived from genetic fingerprints to viable cells with intact cell membranes. however, pma treatment cannot completely suppress polymerase chain reaction (pcr) amplification when the targeted gene is too short. pma treatment in combination with two-step nested pcr was designed to overcome this problem. four experiments were performed to determine the limitation of pma treatment and to evaluate the suitability of the method by ap ... | 2010 | 20024544 |
| characterization of a para-nitrophenol catabolic cluster in pseudomonas sp. strain nyz402 and construction of an engineered strain capable of simultaneously mineralizing both para- and ortho-nitrophenols. | pseudomonas sp. strain nyz402 was isolated for its ability to grow on para-nitrophenol (pnp) as a sole source of carbon, nitrogen, and energy, and was shown to degrade pnp via an oxidization pathway. this strain was also capable of growing on hydroquinone or catechol. a 15, 818 bp dna fragment extending from a 800-bp dna fragment of hydroxyquinol 1,2-dioxygenase gene (pnpg) was obtained by genome walking. sequence analysis indicated that the pnp catabolic gene cluster (pnpabcdefg) in this fragme ... | 2010 | 20049512 |
| enzymatic flow injection method for rapid determination of choline in urine with electrochemiluminescence detection. | in order to determine trace choline in human urine, a flow injection analysis (fia) system has been developed by coupling an enzyme reactor with an electrochemiluminescence (ecl) detector. the enzyme reactor is prepared by covalently immobilizing choline oxidase (chox) onto the aminopropyl-controlled pore glass beads, which are then carefully packed into a micro column. the enzyme reactor catalyzes the production of hydrogen peroxide that is in direct proportion to the concentration of choline. ... | 2010 | 20051326 |
| first reported case of alcaligenes faecalis peritonitis. | | 2010 | 20056996 |
| trends in antimicrobial susceptibility of gram-negative bacteria isolated from blood in jakarta from 2002 to 2008. | this study examined the susceptibility of gram-negative bacteria in the bloodstream to antimicrobials with the aim of providing information relevant to the guidance of therapy. | 2009 | 20061679 |