| complete cryspovirus genome sequences from cryptosporidium parvum isolate iowa. | bisegmented dsrna viruses that infect most or all isolates of apicomplexan parasite cryptosporidium parvum are currently assigned to a single species, cryptosporidium parvum virus 1, in genus cryspovirus, family partitiviridae. an analysis of existing sequence data suggested that the complete sequences of both cryspovirus genome segments, dsrna1 and dsrna2, had yet to be determined. we therefore set out to accomplish this for the virus strain that infects c. parvum isolate iowa. the results sugg ... | 2017 | 28477174 |
| structurally linked dynamics in lactate dehydrogenases of evolutionarily distinct species. | we present new findings about how primary and secondary structure affects the role of fast protein motions in the reaction coordinates of enzymatic reactions. using transition path sampling and committor distribution analysis, we examined the difference in the role of these fast protein motions in the reaction coordinate of lactate dehydrogenases (ldhs) of apicomplexa organisms plasmodium falciparum and cryptosporidium parvum. having evolved separately from a common malate dehydrogenase ancestor ... | 2017 | 28445027 |
| characterization of host cell mutants significantly resistant to cryptosporidium parvum infection. | cryptosporidium parvum is a parasitic protist and a causative agent of mild-to-severe diarrheal diseases in humans and animals. despite its globally recognized importance, knowledge on the mechanism of parasite invasion and molecular interactions between host cells and the parasite is limited. here, we report the establishment of 43 mutant cell lines derived from hct-8 cells by uv-induced mutagenesis and the characterization of three mutants with significantly reduced susceptibility to cryptospo ... | 2017 | 28432811 |