| [possibilities for the differential diagnosis of tuberculosis and nontuberculous lung diseases complicated by spontaneous pneumothorax]. | | 1988 | 3129713 |
| [healing of the lung caverns based on data from bacteriological and morphological studies]. | | 1988 | 3129714 |
| [improved chemoprophylaxis of children from groups at increased risk of tuberculosis morbidity]. | | 1988 | 3129715 |
| serial lactate determinations in tuberculous meningitis. | lactate levels in the cerebrospinal fluid (csf) were determined in 21 patients with culture-proved tuberculous meningitis (tbm). all patients showed uniformly high lactate level (greater than or equal to 3.9 mmol/l). neither the clinical stage of tbm nor the prognosis was related to the csf lactate concentration. repeated csf studies demonstrated persistently high lactate levels in the early stage of illness despite adequate antituberculous therapy. | 1988 | 3129781 |
| pyrazinamidase activity of mycobacterium tuberculosis--a test of sensitivity to pyrazinamide. | pyrazinamidase activity has been found to correlate with pyrazinamide sensitivity in strains of mycobacterium tuberculosis. in vitro sensitivity to pyrazinamide in acidified löwenstein-jensen medium, and pyrazinamidase activity by the wayne method, were determined in 378 clinical isolates of m. tuberculosis. a close correlation was observed between the results of both tests. this method of detecting pyrazinamidase activity was found to be a rapid, simple and reliable substitute for pyrazinamide ... | 1987 | 3129848 |
| pyrazinamide is not effective against intracellularly growing mycobacterium tuberculosis. | | 1988 | 3129990 |
| the impact of respiratory failure on the diagnosis of tuberculosis. | six patients with hypoxic respiratory failure (arterial po2/alveolar po2 less than 0.50) resulting from active tuberculosis were evaluated to assess the impact of respiratory failure on the diagnosis of the underlying tuberculosis. all patients demonstrated anemia (hematocrit [mean +/- sem], 0.29 +/- 0.01 [29.0% +/- 1.0%]) and hypoalbuminemia (serum albumin, 22 +/- 2 g/l [2.2 +/- 0.2 g/dl]) and noted an illness longer than one week. findings on chest roentgenograms varied from a miliary pattern, ... | 1988 | 3130000 |
| [mycobacterioses in patients with hiv infection]. | mycobacterial infections were confirmed in 629 hiv-infected persons (1.4%). five patients had a generalized infection with mycobacterium avium complex (mac), four an extrapulmonary tuberculosis caused by mycobacterium tuberculosis (m. tbc). in general, the tuberculosis was the first severe opportunistic infection, while infections with mac were more frequent in patients with already manifest aids. common to all patients were a septic temperature and definite shift to the left of neutrophil granu ... | 1988 | 3130241 |
| turnover of phospholipids in whole cells and membranes of m. tuberculosis h37rv. | | 1987 | 3130308 |
| isolation, identification and drug sensitivity pattern of mycobacterial strains isolated from human patients. | | 1987 | 3130312 |
| serological studies of mycobacterium tuberculosis infection in north indian population. | | 1987 | 3130373 |
| consensus: management of tuberculin-positive children without evidence of disease. | | 1988 | 3130606 |
| gamma interferon in experimental murine tuberculosis. | | 1988 | 3130917 |
| [evaluation of the effectiveness of tuberculosis control measures in districts of the epidemiological survey in 1979]. | | 1987 | 3131028 |
| [fully supervised ambulatory short-course chemotherapy with an intermittent regimen for newly diagnosed smear-positive pulmonary tuberculosis]. | | 1987 | 3131029 |
| in vivo-activated mononuclear phagocytes and protective immunity to chlamydiae in mice. | peritoneal macrophages (m phi s) collected from chlamydia psittaci 6bc-immune mice after intraperitoneal challenge with 10(6) 6bc (immune-boosted [ib] m phi s) were compared by various functional criteria with other in vivo- and in vitro-activated m phi populations. while casein-, protease peptone-, and thioglycolate (thio)-elicited m phi s were equally susceptible to in vitro infection with 6bc, ib m phi s did not support chlamydial growth and m phi s from mycobacterium tuberculosis bcg- or lis ... | 1988 | 3131246 |
| serological activity of natural disaccharide octyl bovine serum albumin (nd-o-bsa) in sera from patients with leprosy, tuberculosis, and normal controls. | we studied the natural disaccharide-octyl-bovine serum albumin (nd-o-bsa) enzyme-linked immunosorbent assay (elisa) in sera from 151 leprosy patients, 20 tuberculosis patients, and 42 normal persons from a nonendemic area. the three elisas, whole mycobacterium leprae (wml), phenolic glycolipid-i (pgl-i), and nd-o-bsa, are all highly sensitive for detecting antibodies against m. leprae. the results indicate that the serological activity has highly significant, positive correlations among the thre ... | 1988 | 3131466 |
| [tuberculous arthritis following trauma]. | | 1988 | 3131939 |
| the serodiagnosis of tuberculosis: a comparison of an enzyme-linked immunosorbent assay and a solid-phase radioimmunoassay. | antibodies to a cytosolic fraction of mycobacterium tuberculosis in sera from 104 patients with pulmonary tuberculosis and 141 age-matched healthy control subjects were quantitated by solid-phase radioimmunoassay (spria) and an enzyme-linked immunosorbent assay (elisa) technique. the spria gave a greater discrimination than elisa: in the former test 92% of patients had antibody levels in excess of those occurring in 97% of controls, while the corresponding figure with the latter was only 73%. | 1988 | 3131980 |
| improved isolation of mycobacteria other than mycobacterium tuberculosis on isoniazid-containing löwenstein-jensen medium. | the benefit of including isoniazid-containing löwenstein-jensen medium for primary isolation of mycobacteria was evaluated in 3,726 clinical specimens. this media increased the primary isolation of mycobacteria other than mycobacterium tuberculosis by 9.2%, facilitated macroscopical reading and aided presumptive identification of the isolated mycobacteria. | 1988 | 3132376 |
| the processing and presentation of mycobacterial antigens by human monocytes. | the processing and presentation of whole irradiated mycobacterium tuberculosis (mtb gamma) and its purified protein derivative (ppd) by the peripheral blood monocytes from healthy bacillus calmette guérin (bcg)-vaccinated individuals was investigated. to study processing and presentation as events distinct from t cell recognition and proliferation, monocytes were pulsed with antigens for varying time intervals and fixed. the kinetics of presentation indicate that up to 2 h was required for effec ... | 1988 | 3132396 |
| [study of the sputum for mycobacterium tuberculosis]. | | 1988 | 3132403 |
| application of floatation method in pulmonary tuberculosis centres. | | 1987 | 3132420 |
| immune responses to sulpholipids of mycobacterium tuberculosis h37 rv. | | 1987 | 3132424 |
| [congenital tuberculosis]. | | 1988 | 3132620 |
| stress proteins are immune targets in leprosy and tuberculosis. | to understand the immune response to infection by tuberculosis and leprosy bacilli and to develop improved vaccines, the nature of antigens that are involved in humoral and cell-mediated immunity was investigated. we have determined that five immunodominant protein antigens under study are homologues of stress proteins. this finding and observations with other pathogens suggest that infectious agents may respond to the host environment by producing stress proteins and that these proteins can be ... | 1988 | 3132709 |
| the detection by immunoassay of antibody to mycobacterial antigens and mycobacterial antigens in bronchoalveolar lavage fluid from patients with tuberculosis and control subjects. | bronchoalveolar lavage (bal) samples from patients with pulmonary tuberculosis and from control subjects were studied using direct and inhibition enzyme-linked immunosorbent assay (elisa) for the detection of igg and iga antibody to mycobacterium tuberculosis culture filtrate and purified antigen 5 and for the detection of mycobacterial antigens. the igg and iga antibodies were found in the majority of samples from tuberculous patients. the igg titers were higher than were iga titers. substantia ... | 1988 | 3133161 |
| prediction and identification of an hla-dr-restricted t cell determinant in the 19-kda protein of mycobacterium tuberculosis. | an allele-specific motif has been identified in the sequence of several peptides which are recognized by t cells in association with hla-dr1. in order to test the predictive values of such a motif we analyzed the 19-kda antigen from mycobacterium tuberculosis and identified a sequence containing a pattern characteristic of dr1 restriction. peripheral blood mononuclear leukocytes from every dr1 and 4 individual tested responded to the corresponding synthetic peptide. nine other donors, constituti ... | 1988 | 3133233 |
| primary drug resistance of mycobacterium tuberculosis to antituberculosis drugs. | | 1988 | 3133501 |
| [microbiological research on fluid obtained during bronchoalveolar lavage of patients with circumscribed pulmonary tuberculosis]. | | 1988 | 3133650 |
| [dynamics of the shedding of bacterial and ultrafine forms of mycobacteria during the chemotherapy of patients with pulmonary tuberculosis]. | the specific features of bacterial excretion by patients with pulmonary tuberculosis in the process of chemotherapy, depending on the duration of treatment, have been studied, and the time-course of the excretion of ultramicro forms of mycobacteria by patients with and without caverns in the lungs in the process of chemotherapy has been followed. the results of the detection of m. tuberculosis ultramicro forms with the use of the biological and bacteriological methods indicate that both these me ... | 1988 | 3133915 |
| susceptibility of streptomycin-resistant mycobacterium tuberculosis strains to amikacin. | a total of 585 mycobacterium tuberculosis strains isolated during a three-year period in sweden were tested for in vitro susceptibility to amikacin in comparison with streptomycin. resistance to streptomycin was seen in 27 of the isolates (4.6%). these isolates had been obtained from 22 patients, a majority of which were non-european immigrants. all but one of the streptomycin-resistant isolates were susceptible to amikacin. none of 263 streptomycin-susceptible isolates tested was resistant to a ... | 1988 | 3134211 |
| [detection of tuberculosis in patients with chronic nonspecific diseases of the respiratory organs]. | | 1988 | 3134572 |
| use of radiolabelling and fluorescent staining methods in the evaluation of viability and drug susceptibility testing of mycobacterial cultures. | the utility of radiolabelling and fluorescent staining was investigated for the assessment of viability and drug susceptibility testing of mycobacteria. fast growing mycobacterium w was used as a test organism for optimization of experimental conditions. a fairly good correlation was found between the profiles obtained for radiolabelling with 14c-acetate and fluorescein diacetate-ethidium bromide (fda-eb) staining of heat-killed and live m.w. the reliability of two methods for determining the vi ... | 1988 | 3134768 |
| disseminated tuberculosis complicated by the hemophagocytic syndrome. | a 43-year-old polynesian presented with fever, abnormal liver function tests, bilateral pulmonary infiltrates, and a progressive neutropenia and thrombocytopenia. bone marrow examination showed extensive and striking hemophagocytosis which raised the diagnosis of malignant histiocytosis. mycobacterium tuberculosis was subsequently cultured from both urine and bone marrow. hemophagocytosis is now a recognised association of several infections and we draw attention to this finding, particularly as ... | 1988 | 3134883 |
| evaluation of a dual-antigen elisa test for the serodiagnosis of tuberculosis. | two antigen preparations from mycobacterium tuberculosis and m. bovis, respectively, were used in an enzyme-linked immunosorbent assay (elisa) to quantitate the level of igg antibodies in samples of 169 sera obtained in india from 75 patients with pulmonary tuberculosis and from 94 controls. the results of the two elisa tests were integrated using defined criteria. all the controls were correctly classified by this dual-elisa approach and only two patients were categorized as false negatives. th ... | 1988 | 3135125 |
| distinction of mycobacterium tuberculosis from other mycobacteria through dna hybridization. | | 1988 | 3135262 |
| antibody responses against mycobacterium tuberculosis in 11 strains of inbred mice: novel monoclonal antibody specificities generated by fusions, using spleens from balb.b10 and cba/j mice. | eleven strains of inbred mice were immunized with a culture filtrate of mycobacterium tuberculosis h37rv, and the quality of the antibody responses was determined by immunoblotting. the quantity of mycobacterial antigen used for each immunization ranged from 6 to 750 micrograms per inoculum. the culture filtrate of m. tuberculosis was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrotransferred to nitrocellulose filters. immunoblotting results were obtained with ... | 1988 | 3135266 |
| false-positive reactions with enzyme-linked immunosorbent assay of mycobacterium tuberculosis antigens in pleural fluid. | the value of enzyme-linked immunosorbent assay (elisa) for the diagnosis of tuberculous pleural effusion has not been defined. we performed elisa by a double antibody sandwich technique with anti-bcg antibody in the solid phase to detect mycobacterium tuberculosis antigen in pleural fluid from 36 patients with pleural effusion (tuberculosis 15, lung cancer 12, miscellaneous 9). pleural fluids from 12 of the tuberculosis patients, 12 of the cancer patients and one patient in the miscellaneous gro ... | 1988 | 3135407 |
| hip arthroplasty in quiescent mycobacterial infection of hip. | three patients underwent hip arthroplasty--one total hip replacement and two, thompson's hemiarthroplasty. the diagnosis was degenerative joint disease and subcapital fracture of the femur, respectively. histologic and bacteriologic examination revealed mycobacterial infection. none of the patients had a past history of mycobacterial infection. in one instance, there was a reactivation of the infection eight months following surgery, which was treated successfully by antituberculosis drugs. | 1986 | 3135526 |
| on the "cause" of tuberculosis. | in epidemiology, tuberculosis is currently considered to have multiple causes and "cause" is used for all secondary, aggravating and conditional factors in its pathogenesis. the tubercle bacillus is the cause of tuberculosis, and without it tuberculosis cannot occur. the failure to differentiate the cause from noncausative associated factors has wide and serious implications. it is retrogressive, scientifically inaccurate and to be avoided for the sake of logic and precision in scientific commun ... | 1987 | 3135527 |
| [diagnosis and evaluation of pulmonary tuberculosis activity]. | | 1988 | 3135665 |
| [chemotherapy of destructive tuberculosis with the study of the total bacteriostatic activity of the resected lung tissue]. | | 1988 | 3135669 |
| aspects of the antituberculous activity of 27753-rp, a new semisynthetic derivative of griselimycine. | the antituberculous activity of 27753-rp (rp), a new semisynthetic derivative of griselimycine, was determined both in vitro and in vivo, and its activity against atypical mycobacteria was also studied in vitro. 1) the minimal inhibitory concentration of rp against h37rv and its mutants resistant to streptomycin, isoniazide, kanamycin, rifampicin and enviomycin was 0.5 microgram/ml in liquid media, showing no cross-resistance. however, loss of potency occurred in ogawa's egg media, and 50 microg ... | 1987 | 3135829 |
| the role of chlamydiae in epididymitis. | in 21 men suffering from acute or chronic epididymitis who underwent scrotal surgical exploration, diagnostic microbiological studies consisting in cultures of the epididymal tissues and mid-stream urine specimens, as well as examination of the urethral swabs--before and after prostatic massage--were performed. laboratory tests revealed that 55 per cent of the patients younger than 40 years were infected by chlamydia trachomatis, and 36 per cent by urinary tract bacteria. on the other hand, in o ... | 1988 | 3136090 |
| specific detection of mycobacterium tuberculosis in radiometric cultures by using an immunoassay for antigen 5. | | 1988 | 3136212 |
| [recent trends of bone inflammations]. | | 1988 | 3136216 |
| [long-term results of short-course chemotherapy in pulmonary tuberculosis (final report). the third study: comparative study of two regimens, 6rhz and -6rhs (treatment was continued for 6 months after negative conversion of sputum by culture)]. | | 1988 | 3136270 |
| [mycobacteria encountered in djibouti]. | the authors studied 198 strains of mycobacteriae from different sources, but mainly from bronchial secretion and adenoid pus or poundings. because the preponderance of peripheral adenoid tuberculosis, we might fear a focus of mycobacterium bovis. but this study gives leave to state positively that mycobacterium tuberculosis hominis is almost exclusively the responsible and consequent by that there is an human reservoir almost exclusive. on the other hand, study of primary and secondary resistanc ... | 1988 | 3136293 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 35-1988. 63-year-old asplenic man with myelofibrosis, fever, and bloody diarrhea. | | 1988 | 3136332 |
| [sensitivity of 7 mycobacterial species to new quinolones]. | the authors have tested the sensibility to three new quinolones of forty one strains of mycobacteria belonging to seven different species. they have observed, in particular, susceptibility for the species m. tuberculosis, m. fortuitum, m. kansasii, m. marinum and m. xenopi to ciprofloxacin and ofloxacin and a limited sensibility for these same species to pefloxacin. in association with others antibiotics, amikacin, erythromycin, thienamycin, trimethoprim-sulfamethoxazole, the three have shown so ... | 1988 | 3136429 |
| [the virulence of mycobacterium tuberculosis isolated from the sputum and bronchial secretion of patients with specific forms of pulmonary tuberculosis]. | | 1988 | 3136445 |
| [studies with the bactec 460 system. isolation of mycobacterium tuberculosis from sputum. comparison of the radiometric with the conventional method after pretreatment with pancreatin-desogen, lauryl sulfate and n-acetyl-l-cysteine-naoh]. | | 1988 | 3136452 |
| radiometric studies on the oxidation of (u-14c) l-amino acids by drug-susceptible and drug-resistant mycobacteria. | | 1987 | 3136534 |
| [psychosocial risk factors in pulmonary tuberculosis and the phenomenon of drug resistance in its treatment]. | | 1988 | 3136589 |
| differences in uptake of mycobacteria by human monocytes: a role for complement. | we investigated the influence of serum factors on the uptake of various species of mycobacteria by human peripheral blood monocytes (pbm). on the basis of the percentage of pbm involved during in vitro uptake, the mycobacteria were of two distinct groups. the mycobacteria of one group, which consisted of mycobacterium avium complex and m. chelonae, were taken up by many pbm; the other group, consisting of m. tuberculosis, m. kansasii, m. fortuitum, and m. gordonae, were taken up by fewer pbm. m. ... | 1988 | 3137162 |
| rapid detection and identification of pathogenic mycobacteria by combining radiometric and nucleic acid probe methods. | the combination of radiometric methodology (bactec 12b) and probe technology for recovery and identification of mycobacteria was studied in two large hospital laboratories. the sediment from vials with positive growth indices was tested with dna probes specific for mycobacterium tuberculosis, mycobacterium avium, and mycobacterium intracellulare. the sensitivity of the radiometric method and the specificity of the probes resulted in a marked reduction in the time to the final report. biochemical ... | 1988 | 3137247 |
| effect of egg yolk on growth of mycobacterium tuberculosis in 7h12 liquid medium. | of 92 drug-resistant mycobacterium tuberculosis strains isolated from sputum specimens, 86 showed growth in two types of 7h12 broth, one with egg yolk and the other without egg yolk. in addition, two strains grew only in plain 7h12 broth without yolk, and four others were recovered only in the medium supplemented with egg yolk. the radiometrically detected growth was higher in the presence of egg yolk, corresponding to a higher number of cfu per milliliter in these cultures. the improvement of g ... | 1988 | 3137248 |
| [trial of a classification system for strains of mycobacterium tuberculosis using phagotypic markers]. | | 1988 | 3137646 |
| [obtaining hybridomas producing monoclonal antibodies with different specificities against mycobacterium tuberculosis of the human and bovine types]. | a procedure for isolation of hybridomes producing monoclonal antibodies (mcab) to tubercle bacilli is described. specificity of the mcabs was studied with the solid phase radioimmune and immunoenzyme tests. supernatant of tubercle bacilli destroyed with ultrasound was used as antigens. the mcabs did not practically react with antigens of the tubercle bacilli atypical forms. five ascitic monoclonal hybridomes were isolated. four of them produced antibodies with selective specificity to antigens o ... | 1988 | 3137905 |
| cell surface analysis of purified protein derivative-induced cytotoxic cells in tuberculous pleurisy. | high nk cell activity was observed in tuberculous pleural effusions from which mycobacterium tuberculosis was cultured. further we observed that cytotoxicity against k562 was augmented by purified protein derivative (ppd). we have now performed the surface analysis of the cells in ppd-induced cytotoxicity using various monoclonal antibodies (mab) (anti-leu 1, anti-leu 7, anti-leu 11, anti-hla-dr). leu 11+ cells have essential cytotoxicity in ppd-induced cytotoxicity, but neither leu 1+ nor leu 7 ... | 1988 | 3138478 |
| [the role of l variants of mycobacteria in the development and clinical course of recurrences of pulmonary tuberculosis]. | | 1988 | 3138685 |
| [use of biseptol for preventing the development of rifampicin-resistant mycobacterium tuberculosis]. | | 1988 | 3138686 |
| much's granules revisited. | | 1987 | 3138799 |
| the variability of mycobacterium tuberculosis in patients with cavitary pulmonary tuberculosis in the course of chemotherapy. | inoculation of guinea pigs with membrane-filtered homogenates prepared from the walls of open cavities in the lungs of experimental animals allowed us to detect invisible (ultra-fine) forms of the tuberculosis agent. the ultra-fine forms of m. tuberculosis were detected directly by electron microscopy, and indirectly by culture on liquid semi-synthetic media and subsequent microscopy, and by injection of the pathological material into the experimental animals. similar results were obtained in pa ... | 1987 | 3138800 |
| bacteriostatic and bactericidal activity of ciprofloxacin and ofloxacin against mycobacterium tuberculosis and mycobacterium avium complex. | mics of ciprofloxacin were lower than mics of ofloxacin for both m. tuberculosis and m. avium complex strains. the mics of both drugs for 41 m. tuberculosis strains were in a narrow range, and significantly lower than the achievable serum concentrations. the mics of ciprofloxacin for 46 m. avium strains were in a wide range, and in only 28% were the broth-determined mics of ciprofloxacin within the same range as for m. tuberculosis and lower than the achievable serum level. when compared with th ... | 1987 | 3138801 |
| neonatal bcg vaccination and mycobacterial cervical adenitis in childhood. | mycobacterial cervical adenitis is an uncommon disease in children in finland. during 10 years, from 1977-1986, its incidence was of the order of 0.3/year/100,000 children. of the 12 bacteriologically verified cases, m. avium-intracellulare was isolated in nine, m. malmoense in two and m. tuberculosis in only one case. neonatal bcg vaccination seemed to protect children against non-tuberculous mycobacterial infection, especially at 1-4 years of age. in sweden, where neonatal bcg vaccination has ... | 1987 | 3138802 |
| [features of bacteria elimination in patients with tuberculosis of the lungs]. | | 1988 | 3138823 |
| [effect of aggravating factors on the effectiveness of the treatment of newly detected patients with destructive pulmonary tuberculosis]. | | 1988 | 3138824 |
| improved detection of mycobacterium avium complex with the bactec radiometric system. | a reconsideration of the laboratory methods used for primary isolation of mycobacteria other than mycobacterium tuberculosis is needed due to the increasingly recognized importance of such mycobacterial infections in immunocompromised patients. one example of this is the severe opportunistic infections caused by mycobacterium avium complex among aids patients. in this study, the bactec radiometric system was compared to conventional culture on solid medium for the detection of m. avium complex i ... | 1988 | 3139355 |
| direct antimicrobial drug susceptibility testing of mycobacterium tuberculosis by the radiometric method. | direct-drug-susceptibility tests were performed on clinical specimens positive for acid-fast bacilli by either ziehl-neelsen or fluorochrome staining. the results of conventional agar dilution (vestal, 1975) and a modified radiometric (bactec) method were compared. a total of 580 smear-positive specimens were tested by the bactec method at three separate sites. three hundred and seventy-seven of these were culture positive for m. tuberculosis, and 343 (91%) yielded acceptable direct-susceptibili ... | 1988 | 3139356 |
| tuberculous peritonitis in an adolescent male. | tuberculosis (tb) is a less common disease in adolescents than in any other age group. however, when it occurs it is extrapulmonary 10% of the time. we report a case of tb peritonitis in an adolescent patient who presented without a history of prior tb or tb exposure. tuberculosis must be included in the differential diagnosis of any occult febrile illness, even in the absence of pulmonary disease. | 1988 | 3139591 |
| antibody and antigen detection for the immunodiagnosis of tuberculosis: why not? what more is needed? where do we stand today? | | 1988 | 3139774 |
| rapid diagnosis of tuberculous meningitis by using an enzyme-linked immunosorbent assay to detect mycobacterial antigen and antibody in cerebrospinal fluid. | rapid diagnostic tests for tuberculous meningitis are urgently needed because delayed treatment increases the already high mortality rate of this disease. direct acid-fast staining of cerebrospinal fluid is the only quick method generally available, but it lacks sensitivity. therefore, we evaluated the use of an enzyme-linked immunosorbent assay (elisa) to mycobacterial antigen and antibody in the cerebrospinal fluid of 29 patients with proven tuberculous meningitis, 83 patients with nontubercul ... | 1988 | 3139775 |
| the secreted antigens of mycobacterium tuberculosis and their relationship to those recognized by the available antibodies. | proteins secreted by strains of mycobacterium tuberculosis during short-term, zinc-sufficient batch culture were identified in order to define antigens likely to be relevant to the pathogenesis of human disease. [35s]methionine-labelled proteins in supernatants of 4-7 d cultures were separated by page under both denaturing and non-denaturing conditions, and the position of labelled material was determined. secreted protein patterns of m. tuberculosis were quite similar to those of bacillus calme ... | 1988 | 3139828 |
| fluorescence histochemical double staining for the localization of intracellular hydrolases and tubercle bacilli. | fluorescence histochemical techniques are described to localize intracellular enzyme and tubercle bacilli in alveolar macrophages obtained from infected guinea pigs. the study demonstrated a double-staining technique using 4-methylumbelliferyl substrates for enzyme localization followed by auramine-o staining for intracellular tubercle bacilli. upon enzyme substrate reaction, released 4-mu remains retained inside the cell and gives a green fluorescence, whereas tubercle bacilli give a yellow flu ... | 1988 | 3139996 |
| dna probes for mycobacteria. i. isolation of dna probes for the identification of mycobacterium tuberculosis complex and for mycobacteria other than tuberculosis (mott). | traditional methods used in identifying mycobacteria such as acid-fast bacillus stains and culture are often time-consuming, insensitive and non-specific. the isolation of dna probes, coupled to a non-radioactive, e.g. biotin-based detection system, have the potential to foster the development of clinical assays for mycobacterium tuberculosis and mycobacteria other than tuberculosis (mott) that are rapid, sensitive and specific. to this end, we have isolated two different probes: one which is sp ... | 1988 | 3139997 |
| booster effect of tuberculin testing in healthy 6-year-old school children vaccinated with bacillus calmette-guérin at birth in santiago, chile. | in order to determine whether tuberculin testing caused a booster effect in children vaccinated with bacillus calmette-guérin (bcg) at birth, we studied forty 6-year-olds by repeat tuberculin testing 2 weeks later on the contralateral forearm. all children were healthy and had no known exposure to tuberculosis. none of the children had a history of mycobacteriosis other than tuberculosis. the mean induration was 2.3 +/- 1.8 mm for the first tuberculin reaction and 7.6 +/- 3.3 mm for the second t ... | 1988 | 3140208 |
| erythema induratum (of bazin) in a patient with endometrial tuberculosis. | we report erythema induratum in a patient who was found to have active endometrial tuberculosis. this case report emphasizes the importance of an exhaustive search for active tuberculosis in patients with erythema induratum, especially in countries where tuberculosis is prevalent, as the indiscriminate treatment of erythema induratum with steroids may be harmful. | 1988 | 3140229 |
| new imidazo [4,5-b]pyridine carboxylic acids and their derivatives with expected tuberculostatic activity. | | 1988 | 3140255 |
| circulating immune complexes in patients with advanced pulmonary tuberculosis detected by a polyethylene glycol precipitation-complement consuming test (peg-cc test). | | 1988 | 3140341 |
| [tuberculous monoarthritis]. | | 1988 | 3140344 |
| effects of isoniazid and of ceforanide against virulent tubercle bacilli in cultured human macrophages. | isoniazid (inh) is said to inhibit tubercle bacilli equally well in vivo and in vitro, and to be mycobactericidal. ceforanide (cef) can inhibit tubercle bacilli in vitro but has been found ineffective clinically. these two drugs were tested against virulent tubercle bacilli in cultured human macrophages (mp), partly to compare the results with clinical experience, and partly for a better understanding of antituberculosis activities of these drugs in human beings. inh had the same minimal inhibit ... | 1988 | 3140456 |
| the detection of mycobacterial dna sequences in uncultured clinical specimens with cloned mycobacterium tuberculosis dna as probes. | a plasmid dna library was constructed from restriction endonuclease digested genomic deoxyribonucleic acid (dna) of a virulent strain of mycobacterium tuberculosis isolated from sputum of a patient. the sensitivity and specificity of two of the cloned dna fragments in detecting m. tuberculosis and its related dna sequences were analysed by dna-to-dna hybridization. the level of detection was determined to be 50 picograms of m. tuberculosis dna, which is approximately equivalent to 10,000 mycobac ... | 1988 | 3140457 |
| primary antituberculosis drug resistance and acquired rifampicin resistance in gujarat, india. | the prevalence of primary antituberculosis drug resistance in gujarat, as studied between 1983 and 1986, was found to be significantly high, especially for isoniazid (13.9%) and streptomycin (7.4%). primary rifampicin and pyrazinamide resistance were not detected in any strain. the prevalence of rifampicin resistance among treatment failure and relapse cases of pulmonary tuberculosis increased significantly from 2.8% in 1980 to 37.3% in 1986. in about 95% of the rifampicin resistant strains ther ... | 1988 | 3140458 |
| bacteriophage typing of mycobacterium tuberculosis cultures from incidents of suspected laboratory cross-contamination. | bacteriophage typing was performed on 235 mycobacterium tuberculosis cultures submitted from 31 laboratories. in each instance, either the attending physician questioned the misdiagnosis of tuberculosis or the laboratory supervisor suspected that laboratory cross-contamination had occurred. phage typing data confirmed these suspicions. phage typing is a useful adjunct in the investigation of suspected cross-contamination of laboratory cultures of m. tuberculosis. | 1988 | 3140459 |
| tuberculous rheumatism (poncet's disease) in a child. | a 2-year-old boy who had increasing difficulty walking and had large, warm, sterile knee and ankle effusions was found to have active vertebral tuberculosis and a large prevertebral abscess. lymphocyte proliferation assays demonstrated increased purified protein derivative-induced reactivity of synovial fluid lymphocytes compared with peripheral blood lymphocytes. the arthritis responded rapidly to antituberculous and antiinflammatory drugs. this patient's disease represented an example of tuber ... | 1988 | 3140822 |
| preliminary evaluation of a mycobacterium tuberculosis phenolglycolipid antigen in the serologic diagnosis of tuberculosis. | | 1988 | 3140845 |
| characterization of fibronectin-binding antigens released by mycobacterium tuberculosis and mycobacterium bovis bcg. | fibronectin (fn)-binding antigens are prominent components of short-term culture supernatants of mycobacterium tuberculosis. in 3-day-old supernatants, a 30-kilodalton (kda) protein was identified as the major fn-binding molecule. in 21-day-old supernatants, fn bound to a double protein band of 30 and 31 kda, as well as to a group of antigens of larger molecular mass (57 to 60 kda). fn-binding molecules in this size range, but not of 30 to 31 kda, were also found in sonicates. we showed that the ... | 1988 | 3141278 |
| induction of nonspecific acquired resistance and delayed-type hypersensitivity, but not specific acquired resistance in mice inoculated with killed mycobacterial vaccines. | a number of nonliving mycobacterial preparations were tested in vivo for their capacity to generate various relevant parameters of cellular immunity. all preparations tested had some detectable activity in raising resistance to challenge with mycobacterium tuberculosis or with listeria monocytogenes and in conferring the ability to mount a delayed-type hypersensitivity response to tuberculin. this report presents the first evidence, however, that none of these preparations were able to generate ... | 1988 | 3141288 |
| identification of mycobacteria from culture by using the gen-probe rapid diagnostic system for mycobacterium avium complex and mycobacterium tuberculosis complex. | commercially available kits (mycobacterium avium complex rapid diagnostic system and mycobacterium tuberculosis complex rapid diagnostic system; gen-probe, inc., san diego, calif.) utilizing nucleic acid hybridization for the rapid identification of members of the m. avium-m. intracellulare complex and m. tuberculosis complex were evaluated by using 339 clinical and american type culture collection (rockville, md.) isolates. the tests, which can be performed in approximately 2 h, use specific [1 ... | 1988 | 3141459 |
| serological responses of patients with lepromatous and tuberculoid leprosy to 30-, 31-, and 32-kilodalton antigens of mycobacterium tuberculosis. | sera from patients with lepromatous and tuberculoid leprosy were examined in immunoblot assays for antibodies to mycobacterium tuberculosis culture filtrate antigens. antibodies to 30- and 31-kilodalton proteins were present in 88 and 81%, respectively, of 16 patients with lepromatous disease and absent in 16 patients with tuberculoid disease. antibodies to a 32-kilodalton protein were found in 12 and 38% of lepromatous and tuberculoid patients, respectively. these reactivities may be useful for ... | 1988 | 3141462 |
| monoclonal antibodies selectively directed against the cell wall surface of mycobacterium tuberculosis. | in the last few years several monoclonal antibodies against mycobacterium tuberculosis have been described, but their use as diagnostic tools has been limited. in this study we describe eight monoclonal antibodies against m. tuberculosis for diagnostic purposes. the monoclonal antibodies were selected after enzyme-linked immunosorbent assay screening with whole bacterial suspensions of mycobacteria and other bacterial species. four monoclonal antibodies (bs100, bs101, bs102, and bs104) reacted w ... | 1988 | 3141465 |
| mycobactins and exochelins of mycobacterium tuberculosis, m. bovis, m. africanum and other related species. | following iron-deficient growth, mycobactins and exochelins were isolated from 11 strains of mycobacterium tuberculosis (including type strains of the virulent h37rv and avirulent h37ra organisms) as well as from m. bovis (one strain), m. bovis bcg (two strains), m. africanum (eight strains) and m. xenopi (one strain) but not from m. microti (one strain). the mycobactins from the tuberculosis group (m. tuberculosis, m. bovis and m. africanum) were identical and could each be resolved into four c ... | 1988 | 3141575 |
| [a case of chilaiditi's syndrome appeared in drug-resistant pulmonary tuberculosis following lobectomy]. | | 1988 | 3141667 |
| [the bactec 460 system. 4. comparison of bactec 460 and an animal experiment for the detection of tuberculosis bacteria]. | | 1988 | 3141914 |
| [the role of broncho-alveolar lavage in the diagnosis of pulmonary diseases]. | | 1988 | 3141939 |
| [evaluation of bk positivity and resistance of mycobacterium tuberculosis 1983-1986 in areas of bosnia and herzegovina]. | | 1988 | 3141940 |
| determination of pyrazinamide mics for mycobacterium tuberculosis at different phs by the radiometric method. | the mics of pyrazinamide (pza) were determined for mycobacterium tuberculosis cultivated under different ph conditions in 7h12 liquid medium. mycobacterial growth was monitored by the radiometric method (bactec system; johnston laboratories, inc., towson, md.). we observed a predictable eightfold difference between the mics determined at ph 5.5 and those determined at ph 5.95. the highest mics for 21 susceptible strains were 50.0 micrograms/ml at ph 5.5 and 400 micrograms/ml at ph 5.95. this eig ... | 1988 | 3142340 |
| rapid susceptibility testing of mycobacterium tuberculosis by bioluminescence assay of mycobacterial atp. | mycobacterial growth was monitored by bioluminescence assay of mycobacterial atp. cultures of mycobacterium tuberculosis h37rv and of 25 clinical isolates of the same species were exposed to serial dilutions of ethambutol, isoniazid, rifampin, and streptomycin. a suppression of atp, indicating growth inhibition, occurred for susceptible but not resistant strains within 5 to 7 days of incubation. breakpoint concentrations between susceptibility and resistance were determined by comparing these re ... | 1988 | 3142348 |