| trichloroethylene mineralization in a fixed-film bioreactor using a pure culture expressing constitutively toluene ortho -monooxygenase. | an aerobic, single-pass, fixed-film bioreactor was designed for the continuous degradation and mineralization of gas-phase trichloroethylene (tce). a pure culture of burkholderia cepacia pr1(23)(tom(23c)), a tn5transposon mutant of b. cepacia g4 that constitutively expresses the tce-degrading enzyme, toluene ortho-monooxygenase (tom), was immobilized on sintered glass (sirantrade mark carriers) and activated carbon. the inert open-pore structures of the sintered glass and the strongly, tce-absor ... | 1997 | 18636577 |
| [isolation and characterization of a phenanthrene-degrading bacterial strain]. | a bacterial strain that degrades phenanthrene was isolated from activated sludge of wastewater treatment plant from a chemical corporation. the strain can utilize phenanthrene as sole source of carbon and energy. it was identified as burkholderia sp. according to morphological and 16s rrna gene sequence analysis. phenanthrene (100 mg x l(-1)) was removed 74.1% within 84 h by this strain. it was suggested that the strain possessed the salicylic acid pathway by lc-ms and uv methods. and the strain ... | 2008 | 18637363 |
| proteomic profiling of cellular targets of lipopolysaccharide-induced signalling in nicotiana tabacum by-2 cells. | plants constantly monitor for pathogen challenge and utilize a diverse array of adaptive defense mechanisms, including differential protein regulation, during pathogen attack. a proteomic analysis of nicotiana tabacum by-2 cells was performed in order to investigate the dynamic changes following perception of bacterial lipopolysaccharides. a multiplexed proteome analysis, employing two-dimensional difference-in-gel-electrophoresis with cydye dige fluors, as well as ruthenium ii tris (bathophenan ... | 2008 | 18638580 |
| cutaneous melioidosis in the tropical top end of australia: a prospective study and review of the literature. | burkholderia pseudomallei is endemic in northern australia, and melioidosis is a common cause of sepsis in the region. | 2008 | 18643756 |
| burkholderia cenocepacia strains isolated from cystic fibrosis patients are apparently more invasive and more virulent than rhizosphere strains. | given the widespread presence of burkholderia cenocepacia in the rhizosphere it is important to determine whether rhizosphere strains are pathogenic for cystic fibrosis patients or not. eighteen b. cenocepacia strains of rhizosphere and clinical origin were typed by multi-locus sequence typing (mlst) analysis and compared for their ability to invade pulmonary epithelial cells and their virulence in a mouse model of airway infection. although there was great variability, clinical strains were the ... | 2008 | 18643926 |
| degradation of dimethyl carboxylic phthalate ester by burkholderia cepacia da2 isolated from marine sediment of south china sea. | burkholderia cepacia da2, isolated from marine sediment of the south china sea, is capable of utilizing dimethyl phthalate (dmp) as the sole source of carbon and energy. during the transformation of dmp in batch culture, its corresponding degradation intermediates were identified as monomethyl phthalate (mmp) and phthalate acid (pa) sequentially over the time of incubation. the biodegradation biochemical pathway of dmp was dmp to mmp and then to pa before mineralization. degradation of dmp by b. ... | 2008 | 18651216 |
| [identification and colonization of an antagonistic endophytic burkholderia cepacia lu10-1 isolated from mulberry]. | to identify and colonize an antagonistic bacterium, lu10-1, isolated from the healthy mulberry. | 2008 | 18652294 |
| gene-expression profiles in murine melioidosis. | melioidosis, caused by the bacterium burkholderia pseudomallei, is a septicemic illness, often associated with pneumonia and bacterial dissemination to distant sites. recently we reported the inflammatory mrna profile in blood leukocytes during human melioidosis. knowledge of the inflammatory gene expression profile in the pulmonary compartment after infection with b. pseudomallei, however, is highly limited. we therefore aimed to characterize the inflammatory mrna profile in the pulmonary and s ... | 2008 | 18653369 |
| inactivation of burkholderia pseudomallei bsaq results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles. | burkholderia pseudomallei, an infectious gram-negative bacterium, is the causative pathogen of melioidosis. in the present study, a b. pseudomallei strain with mutation in the bsaq gene, encoding a structural component of the type iii secretion system (t3ss), was constructed. this bsaq mutation caused a marked decrease in secretion of bope effector and bipd translocator proteins into culture supernatant. the b. pseudomallei bsaq mutant also exhibited decreased efficiencies of plaque formation, i ... | 2008 | 18654761 |
| immobilization of lipases on hydrophobilized zirconia nanoparticles: highly enantioselective and reusable biocatalysts. | our study has demonstrated for the first time that zirconia nanoparticles modified by a simple carboxylic surfactant of a very long alkyl chain can significantly enhance the activity of the immobilized lipases for asymmetric synthesis in organic media. zirconia nanoparticles of ca. 20 nm diameter were grafted with carboxylic surfactant modifiers from tween 85 and erucic acid. the surface of nanoparticles was successfully changed from hydrophilic to hydrophobic. lipases from candida rugosa and ps ... | 2008 | 18656972 |
| evaluation of recombinant antigens for diagnosis of melioidosis. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic to southeast asia and northern australia. clinical manifestations of the disease are diverse, ranging from chronic localized infection to acute septicaemia, with death occurring within 24-48 h after the onset of symptoms. definitive diagnosis of melioidosis involves bacterial culture and identification, with results obtained within 3-4 days. this delayed diagnosis is a major contributing factor to high mortality rates. rap ... | 2008 | 18657105 |
| lung transplant and cystic fibrosis: what's new from the uk and france? | | 2008 | 18663066 |
| tn5530 from burkholderia cepacia strain 2a encodes a chloride channel protein essential for the catabolism of 2,4-dichlorophenoxyacetic acid. | | 2008 | 18665945 |
| detection of n-acyl homoserine lactones using a trai-luxcdabe-based biosensor as a high-throughput screening tool. | bacteria use n-acyl homoserine lactone (ahl) molecules to regulate the expression of genes in a density-dependent manner. several biosensors have been developed and engineered to detect the presence of all types of ahls. | 2008 | 18667064 |
| plant host and sugar alcohol induced exopolysaccharide biosynthesis in the burkholderia cepacia complex. | the species that presently constitute the burkholderia cepacia complex (bcc) have multiple roles; they include soil and water saprophytes, bioremediators, and plant, animal and human pathogens. since the first description of pathogenicity in the bcc was based on sour skin rot of onion bulbs, this study returned to this plant host to investigate the onion-associated phenotype of the bcc. many bcc isolates, which were previously considered to be non-mucoid, produced copious amounts of exopolysacch ... | 2008 | 18667584 |
| functional analysis of the burkholderia cenocepacia j2315 bceaj protein with phosphomannose isomerase and gdp-d-mannose pyrophosphorylase activities. | the bcea(j) gene from the cystic fibrosis isolate burkholderia cenocepacia j2315 encodes a 56-kda bifunctional protein, with phosphomannose isomerase (pmi) and guanosine diphosphate (gdp)-mannose pyrophosphorylase (gmp) activities, a new member of the poorly characterised type ii pmi class of proteins. due to the lack of homology between the type ii pmis and the human pmi, this class of proteins are being regarded as interesting potential targets to develop new antimicrobials. the bcea(j) protei ... | 2008 | 18668237 |
| isolation and structural characterization of capistruin, a lasso peptide predicted from the genome sequence of burkholderia thailandensis e264. | lasso peptides are a structurally unique class of bioactive peptides characterized by a knotted arrangement, where the c-terminus threads through an n-terminal macrolactam ring. although ribosomally synthesized, only the gene cluster for the best studied lasso peptide mccj25 from escherichia coli consisting of the precursor protein mcja and the processing and immunity proteins mcjb, mcjc, and mcjd is known. through genome mining studies, we have identified homologues of all four proteins in burk ... | 2008 | 18671394 |
| the transcription regulator rcom-2 from burkholderia xenovorans is a cysteine-ligated hemoprotein that undergoes a redox-mediated ligand switch. | spectroscopic characterization of the newly discovered heme-pas domain sensor protein bxrcom-2 reveals that this protein undergoes redox-dependent ligand switching and co- and no-induced ligand displacement. the aerobic bacterium burkholderia xenovorans expresses two homologous heme-containing proteins that promote co-dependent transcription in vivo. these regulators of co metabolism, bxrcom-1 and bxrcom-2, are gas-responsive heme-pas domain proteins like mammalian neuronal pas domain protein 2 ... | 2008 | 18672900 |
| tlr 5, but neither tlr2 nor tlr4, is involved in lung epithelial cell response to burkholderia cenocepacia. | burkholderia cenocepacia is known to induce a harmful inflammatory response in the airways of cystic fibrosis patients. toll-like receptors (tlrs) play key roles in sensing microbial-associated molecular patterns and initiating host innate immunity, but their role in the inflammatory response elicited by b. cenocepacia has not been precisely examined. in this study, we evaluated the contribution of tlr2, tlr4 and tlr5 to the signaling pathways triggered by b. cenocepacia in human bronchial epith ... | 2008 | 18680520 |
| a possible link between infection with burkholderia bacteria and systemic lupus erythematosus based on epitope mimicry. | we previously demonstrated that purified polyclonal and monoclonal anti-dsdna antibodies bind a 15-mer peptide aspvtarvlwkashv in elisa and dot blot. this 15-mer peptide partial sequence arvlwkash shares similarity with burkholderia bacterial cytochrome b 561 partial sequence arvlwrath. in this study, we show that purified anti-dsdna antibodies react with burkholderia fungorum bacterial cell lysates in western blot. we used anti-dsdna antibodies to make an anti-dsdna antibodies affinity column a ... | 2008 | 18682819 |
| pseudomonas and aeromonas isolates from domestic reptiles and study of their antimicrobial in vitro sensitivity. | | 2008 | 18683065 |
| the structure and proinflammatory activity of the lipopolysaccharide from burkholderia multivorans and the differences between clonal strains colonizing pre and posttransplanted lungs. | the burkholderia cepacia complex is a group of gram-negative bacteria that are opportunistic pathogens for humans especially in cystic fibrosis patients. lipopolysaccharide (lps) molecules are potent virulence factors of gram-negative bacteria organisms essential for bacterial survival. a complete analysis of the bacterial lipopolysaccharide structure to function relationship is required to understand the chemical basis of the inflammatory process. we have therefore investigated the structures o ... | 2008 | 18684854 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| bacterial metabolism of polychlorinated biphenyls. | microbial metabolism is responsible for the removal of persistent organic pollutants including pcbs from the environment. anaerobic dehalogenation of highly chlorinated congeners in aquatic sediments is an important process, and recent evidence has indicated that dehalococcoides and related organisms are predominantly responsible for this process. such anaerobic dehalogenation generates lower chlorinated congeners which are easily degraded aerobically by enzymes of the biphenyl upper pathway (bp ... | 2008 | 18685266 |
| diversity of the c-terminal portion of the biphenyl dioxygenase large subunit. | the biphenyl dioxygenase (bpdo) catalyses a stereospecific dioxygenation of biphenyl and analogs of it. aside from being involved in the destruction and detoxification of toxic pollutants in soil, in the context of the green chemistry concept, this enzyme is a promising biocatalyst to design new more selective and more environmentally friendly approaches to manufacture fine chemicals. at this time, most of our knowledge about the variability of key residues determining the substrate specificity ... | 2008 | 18685267 |
| role of hemoglobin in improving biodegradation of aromatic contaminants under hypoxic conditions. | genetic engineering of bacteria using the vitreoscilla (bacterial) hemoglobin gene has been used to enhance bioremediation of several compounds which are models for, or are themselves, toxic chemicals which may contaminate soil and water. initial experiments, done mostly in shake flasks, with escherichia coli, burkholderia sp. dnt and pseudomonas aeruginosa demonstrated that expression of vitreoscilla hemoglobin in heterologous hosts can enhance biodegradation of several aromatic compounds as we ... | 2008 | 18685270 |
| clinical problem-solving. beware of first impressions. | | 2008 | 18687644 |
| activation of toll-like receptors by burkholderia pseudomallei. | melioidosis, a lethal tropical infection that is endemic in southeast asia and northern australia, is caused by the saprophytic gram-negative bacterium burkholderia pseudomallei. overall mortality approaches 40% yet little is known about mechanisms of host defense. toll-like receptors (tlrs) are host transmembrane receptors that recognize conserved pathogen molecular patterns and induce an inflammatory response. the lipopolysaccharide (lps) of gram-negative bacteria is a potent inducer of the ho ... | 2008 | 18691413 |
| bacterial endosymbiosis is widely present among zygomycetes but does not contribute to the pathogenesis of mucormycosis. | environmental isolates of the fungus rhizopus have been shown to harbor a bacterial endosymbiont (burkholderia) that produces rhixozin, a plant mycotoxin. we sought to define the role of rhizoxin production by endosymbionts in the pathogenesis of mucormycosis. endosymbiotic bacteria were identified by polymerase chain reaction in 15 (54%) of 28 clinical isolates of zygomycetes, with 33% of the bacterial strains showing 87% identity to burkholderia 16s rdna. the presence of rhizoxin in myclial ex ... | 2008 | 18694335 |
| [chronic bacterial infection and echocardiographic parameters indicative of pulmonary hypertension in patients with cystic fibrosis]. | to examine the relationship between chronic bacterial infection and pulmonary hypertension, using doppler echocardiography, in patients with cystic fibrosis (cf). | 2008 | 18695790 |
| [detection methods and changes in metallo-beta-lactamase-producing gram-negative rod isolates at nagasaki university hospital from 1991 to 2005]. | since plasmid-mediated metallo-beta-lactamase (mbl)-producing pseudimonas aeruginosa was reported in japan, mbl-producing gram-negative rods (gnrs) have emerged worldwide. we developed a way to detect mbl-producing gnrs in routine examination using broth microdilution with the mbl inhibitor sodium mercaptoacetate (sma) in frozen plates. between 1996 and 2005, we evaluated this and other methods, including broth microdilution with another mbl inhibitor dipicolinic acid (dpa) in dry plates, conven ... | 2008 | 18697478 |
| identification and functional characterization of gene components of type vi secretion system in bacterial genomes. | a new secretion system, called the type vi secretion system (t6ss), was recently reported in vibrio cholerae, pseudomonas aeruginosa and burkholderia mallei. a total of 18 genes have been identified to be belonging to this secretion system in v. cholerae. here we attempt to identify presence of t6ss in other bacterial genomes. this includes identification of orthologous sequences, conserved motifs, domains, families, 3d folds, genomic islands containing t6ss components, phylogenetic profiles and ... | 2008 | 18698408 |
| temocillin in cystic fibrosis: a retrospective pilot study. | temocillin is currently used in the treatment of acute pulmonary exacerbations caused by burkholderia cepacia complex and multi-resistant pseudomonas aeruginosa in cystic fibrosis (cf) patients despite little published clinical data. this study assessed if intravenous (iv) antibiotic therapy including temocillin was equivalent to standard combination therapy for an acute exacerbation. | 2008 | 18706868 |
| development of novel animal infection models for the study of acute and chronic burkholderia pseudomallei pulmonary infections. | burkholderia pseudomallei causes the disease melioidosis. the most common clinical presentation of melioidosis is pneumonia which can occur in acute and chronic forms. the tsunami of 2004 demonstrated a new risk factor for the acquisition of melioidosis and resulted in the proposal that direct delivery of b. pseudomallei into the lungs may result in the enhanced ability of this pathogen to cause disease. in the present studies, we present the development and characterization of rat models of acu ... | 2008 | 18707015 |
| relationship between antigenicity and pathogenicity for burkholderia pseudomallei and burkholderia mallei revealed by a large panel of mouse mabs. | burkholderia pseudomallei and burkholderia mallei are two closely related gram-negative bacterial species classified by the cdc as category b biowarfare agents. to develop monoclonal antibodies (mabs) that can recognize as many different strains and/or clinical isolates of these two pathogens as possible, we immunized mice with heat-killed bacterial whole cells and membrane preparations from multiple strains and/or clinical isolates of b. pseudomallei and b. mallei. more than 100 different hybri ... | 2008 | 18707541 |
| glanders. | | 2008 | 18710311 |
| survival of burkholderia pseudomallei in water. | the ability of burkholderia pseudomallei to survive in water likely contributes to its environmental persistence in endemic regions. to determine the physiological adaptations which allow b. pseudomallei to survive in aqueous environments, we performed microarray analyses of b. pseudomallei cultures transferred from luria broth (lb) to distilled water. | 2008 | 18710531 |
| application of ft-ir spectroscopy for control of the medium composition during the biodegradation of nitro aromatic compounds. | previous studies showed that cabbage leaf extract (cle) added to the growth medium can noticeably promote the degradation of nitro aromatic compounds by specific consortium of bacteria upon their growth. for further development of the approach for contaminated soil remediation it was necessary to evaluate the qualitative and/or quantitative composition of different origin cle and their relevance on the growth of explosives-degrading bacteria. six cle (different by species, cultivars and harvesti ... | 2008 | 18712533 |
| deployable laboratory response to emergence of melioidosis in central sri lanka. | a portable molecular diagnostic laboratory was used to provide molecular confirmation of suspected melioidosis cases seen at peradeniya hospital, central sri lanka. soil supernatants from rice field and rubber plantation samples also produced pcr-positive results. these procedures could be used for melioidosis field work in other remote locations. | 2008 | 18716231 |
| burkholderia pseudomallei infection in a cystic fibrosis patient from the caribbean: a case report. | burkholderia pseudomallei is a pathogen identified with increasing frequency in the respiratory tracts of cystic fibrosis (cf) patients from endemic areas such as southeast asia and northern australia. the following report describes the first known reported case in a cf patient from the caribbean attending a north american cf clinic. | 2008 | 18716683 |
| development of selective media for the isolation of yeasts and filamentous fungi from the sputum of adult patients with cystic fibrosis (cf). | yeasts and filamentous fungi are beginning to emerge as significant microbial pathogens in patients with cystic fibrosis (cf), particularly in relation to allergic-type responses, as seen in patients with allergic bronchopulmonary aspergillosis (abpa), aspergillus bronchitis and in invasive fungal disease in lung transplant patients. four fungal media were compared in this study, including sabouraud dextrose agar (sda) and medium b, with and without the addition of selective antibiotics, where a ... | 2008 | 18723404 |
| simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan. | a strategy for simultaneous purification and refolding of proteins overexpressed with an intein tag is described. a recombinant lipase overexpressed in escherichia coli er2566 with the intein tag and obtained as inclusion bodies was solubilized in buffer containing 8 m urea or cetyltrimethylammonium bromide. the solubilized lipase was precipitated with chitosan and the affinity complex of the polymer with the fusion protein was obtained. the intein tag was cleaved with dithiothreitol and the ref ... | 2008 | 18725331 |
| role of methoxypolyethylene glycol on the hydration, activity, conformation and dynamic properties of a lipase in a dry film. | a combined approach based on the use of atr-ft/ir and steady-state fluorescence spectroscopy allowed to shed light on the effects of the additive methoxypolyethylene glycol (mepeg) on the hydration, conformation and dynamic properties of lipase from burkholderia cepacia dehydrated to form a film. spectroscopic data show that the additive has little effect on the structure of the protein; however, h/d exchange kinetic and fluorescence anisotropy suggest a more flexible enzyme molecule when in the ... | 2008 | 18727030 |
| specific anion effects on enzymatic activity in nonaqueous media. | the present work shows that salt anions affect the activity of pseudomonas cepacia lipase both in aqueous and in nonaqueous media (nam) according to a hofmeister series. the biocatalytic assay in water was the hydrolysis of p-nitrophenyl acetate, whereas the esterification between 1-hexyl-beta-d-galactopyranoside and palmitic acid was followed in an organic solvent. the solid lipase preparations to be used in nam were obtained through lyophilization in the presence of concentrated solutions of h ... | 2008 | 18729508 |
| roles and interactions of burkholderia pseudomallei bpsir quorum-sensing system determinants. | the burkholderia pseudomallei quorum-sensing system (qss), designated bpsir, is encoded by five bpsr genes and three bpsi genes. this study investigated the roles and interactions of the qss determinants in terms of gene regulation and protein interaction. we report two novel findings, that bpsr can function as an activator and a repressor for bpsi expression and that bpsr may form homodimers and heterodimers. | 2008 | 18757538 |
| emerging pathogens in cystic fibrosis: ten years of follow-up in a cohort of patients. | in patients with cystic fibrosis (cf), there is an increasing incidence of some uncommon respiratory pathogens, such as burkholderia cepacia complex (bcc), stenotrophomonas maltophilia, and achromobacter xylosoxidans. in order to evaluate the prevalence and the clinical impact of these pathogens, we retrospectively studied a total of 109 patients followed in our center from 1996 to 2006 and reviewed the results of 1,550 sputum samples. the isolation of pseudomonas aeruginosa slightly decreased o ... | 2009 | 18758832 |
| diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots. | forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ... | 2008 | 18759227 |
| a genome level survey of burkholderia pseudomallei immunome expressed during human infection. | burkholderia pseudomallei is the etiological agent of melioidosis, a severe infectious disease of humans and animals. the role of the bacterium's proteins expressed in vivo during human melioidosis continues to remain an enigma. this study's aim was to identify b. pseudomallei target proteins that elicit the humoral immune response in infected humans. a small insert genomic expression library was constructed and immunoscreened to identify peptides that reacted exclusively with melioidosis patien ... | 2008 | 18761419 |
| [cloning and sequence analysis of 1,2,4-trichlorobenzene dioxygenase and dehydrogenase genes]. | pseudomonas nitroreducens j5-1 is able to use monochlorobenzene, 1,2-dichlorobenzene, 1,3-dichlorobenzene and 1,2,4-trichlorobenzene as sole carbon and energy sources, and it differs from those 1,2,4-trichlorobenzene degrading bacteria reported in substrate utilizing characters. pcr technique was used to amplify the genes of chlorobenzene dioxygenase and dehydrogenase of j5-1, and they were named as tcba and tcbb, respectively. homology analysis indicated that these genes and gene products were ... | 2008 | 18763518 |
| burkholderia sabiae sp. nov., isolated from root nodules of mimosa caesalpiniifolia. | two rhizobial strains, br3407(t) and br3405, were isolated from nitrogen-fixing nodules on the roots of mimosa caesalpiniifolia, a legume tree native to brazil. on the basis of 16s rrna gene sequence similarities, both strains were shown previously to belong to the genus burkholderia. a polyphasic approach, including dna-dna hybridizations, pulsed-field gel electrophoresis of whole-genome dna profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical charac ... | 2008 | 18768625 |
| effect of bacterial interference on biofilm development by legionella pneumophila. | in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ... | 2008 | 18769851 |
| isolation of dieldrin- and endrin-degrading bacteria using 1,2-epoxycyclohexane as a structural analog of both compounds. | this report describes the selective isolation of dieldrin- and endrin-degrading bacteria from soil with high degradation activity toward dieldrin and endrin. several enrichment cultures from the soil were arranged with several structural analogs of dieldrin and endrin as a growth substrate and examined for their degradation activities toward dieldrin and endrin. an enrichment culture with 1,2-epoxycyclohexane (ech) was found to aerobically degrade dieldrin and endrin. denaturing gradient gel ele ... | 2008 | 18769917 |
| burkholderia cenocepacia j2315 acyl carrier protein: a potential target for antimicrobials' development? | this work describes the isolation and characterization of an acyl carrier protein (acp) mutant from burkholderia cenocepacia j2315, a strain of the burkholderia cepacia complex (bcc). bcc comprises at least 9 species that emerged as opportunistic pathogens able to cause life-threatening infections, particularly severe among cystic fibrosis patients. bacterial acps are the donors of the acyl moiety involved in the biosynthesis of fatty acids, which play a central role in metabolism. the mutant wa ... | 2008 | 18771721 |
| thermotemporal dynamics of contaminant bacteria and antimicrobials in extended porcine semen. | bacterial contamination of extended porcine semen has been associated with deleterious effects on both semen quality and sow fertility. retrospective, prospective and in vitro studies were performed to delineate the prevalence and behavior of certain bacterial contaminants in extended semen, and antimicrobial pharmacodynamics in various semen diluents. retrospective review of extended semen samples submitted from north american boar studs for microbiological screening at the university of pennsy ... | 2008 | 18774598 |
| a functional phenylacetic acid catabolic pathway is required for full pathogenicity of burkholderia cenocepacia in the caenorhabditis elegans host model. | burkholderia cenocepacia is a member of the burkholderia cepacia complex, a group of metabolically versatile bacteria that have emerged as opportunistic pathogens in cystic fibrosis and immunocompromised patients. previously a screen of transposon mutants in a rat pulmonary infection model identified an attenuated mutant with an insertion in paae, a gene related to the phenylacetic acid (pa) catabolic pathway. in this study, we characterized gene clusters involved in the pa degradation pathway o ... | 2008 | 18776009 |
| novel oxidized derivatives of antifungal pyrrolnitrin from the bacterium burkholderia cepacia k87. | the screening of antifungal active compounds from the fermentation extracts of soil-borne bacterium burkholderia cepacia k87 afforded pyrrolnitrin (1) and two new pyrrolnitrin analogs, 3-chloro-4-(3-chloro-2-nitrophenyl)-5-methoxy-3-pyrrolin-2-one (2) and 4-chloro-3-(3-chloro-2-nitrophenyl)-5-methoxy-3-pyrrolin-2-one (3). pyrrolnitrin showed strong antifungal activity against rhizoctonia solani but the analogs (2 and 3) were found to be marginally active. the isolates, 2 and 3, are believed to b ... | 2008 | 18776654 |
| dose-response model for burkholderia pseudomallei (melioidosis). | the objective of this study was development of a dose-response model for exposure to burkholderia pseudomallei in different animal hosts and analysis of the results. the data sets with which the model was developed were taken from the open literature. | 2008 | 18778292 |
| burkholderia thailandensis as a model system for the study of the virulence-associated type iii secretion system of burkholderia pseudomallei. | burkholderia pseudomallei is a bacterial pathogen that causes a broad spectrum of clinical symptoms collectively known as melioidosis. since it can be acquired by inhalation and is difficult to eradicate due to its resistance to a wide group of antibiotics and capacity for latency, work with b. pseudomallei requires a biosafety level 3 (bsl-3) containment facility. the bsa (burkholderia secretion apparatus)-encoded type iii secretion system (ttss) has been shown to be required for its full virul ... | 2008 | 18779342 |
| non-septicemic melioidosis presenting as cardiac tamponade. | melioidosis is endemic in taiwan. it is caused by infection with burkholderia pseudomallei. a prolonged course of oral eradication therapy to avoid relapse after an intensive intravenous therapy is recommended to treat melioidosis. melioidosis with cardiac involvement is rare and is often combined with septicemia, for which the mortality rate is 20-60%. the initial clinical presentations of melioidosis mimic mycobacterium tuberculosis infection, which is the most common etiology of bacterial per ... | 2008 | 18784243 |
| potential plant growth promoting traits and bioacidulation of rock phosphate by thiosulfate oxidizing bacteria isolated from crop plants. | thiosulfate oxidizing bacteria isolated from crop plants were tested for their traits related to plant growth promotion and their ability to solubilize morocco rock phosphate (rp) through oxidation of thiosulfate to sulfuric acid. all the tested strains grew in nfb medium (except dyella ginsengisoli) and possessed beta-1,3 glucanase activity (except burkholderia kururiensis). of the fourteen tested strains, 2 were found positive for siderophore production, 3 each for phytohormones (iaa), and sal ... | 2008 | 18785656 |
| microbiological safety of bottled mineral water in patients susceptible to infections. | we evaluated the microbiological safety of bottled mineral water products commercially available in japan. of 10 bottled mineral water products manufactured in japan, no bacteria or fungi were detected in 9 (90%), but 1 (10%) contained 1.8x10(3) colony-forming units (cfu)/ml. of 12 bottled mineral water products manufactured in the eu, 11 (91.7%) contained 23-3.5x10(4) cfu/ml. on the other hand, of 5 bottled mineral water products manufactured in north america, 2 (40%) contained 2.3x10(2)-2.5x10 ... | 2008 | 18787316 |
| recyclable chaperone-conjugated magnetic beads for in vitro refolding of burkholderia cepacia lipase. | polymer-coated magnetic beads have become widely used in biological applications because of their facile recovery and easily modifiable surface. herein, we report the application of magnetic beads to in vitro refolding of b. cepacia lipase. magnetic particles (fe3o4) prepared by co-precipitation of fe2+ and fe3+ ions under basic conditions were subsequently coated with carboxylic acid-containing polystyrene by emulsion polymerization. the polymer-coated magnetic beads were then conjugated with m ... | 2009 | 18791662 |
| genomics of pyrrolnitrin biosynthetic loci: evidence for conservation and whole-operon mobility within gram-negative bacteria. | pyrrolnitrin (prn) is a tryptophan-derived secondary metabolite produced by a narrow range of gram-negative bacteria. the prn biosynthesis by rhizobacteria presumably has a key role in their life strategies and in the biocontrol of plant diseases. the biosynthetic operon that encodes the pathway that converts tryptophan to prn is composed of four genes, prna through d, whose diversity, genomic context and spread over bacterial genomes are poorly understood. therefore, we launched an endeavour ai ... | 2009 | 18793314 |
| swarming motility: a multicellular behaviour conferring antimicrobial resistance. | swarming is a type of social motility allowing the migration of highly differentiated bacterial cells. swarming shares many similarities with biofilm communities, which are notable for their high resistance to antimicrobial agents. we investigate here if the swarming behaviour could also be associated with a widespread antimicrobial resistant phenotype. challenged with 13 antibiotics from various classes, swarm cells of pseudomonas aeruginosa, escherichia coli, serratia marcescens, burkholderia ... | 2009 | 18793317 |
| genomematcher: a graphical user interface for dna sequence comparison. | the number of available genome sequences is increasing, and easy-to-use software that enables efficient comparative analysis is needed. | 2008 | 18793444 |
| endophytic colonization of rice (oryza sativa l.) by the diazotrophic bacterium burkholderia kururiensis and its ability to enhance plant growth. | burkholderia kururiensis is a diazotrophic bacterium originally isolated from a polluted aquifer environment and presents a high level of similarity with the rice endophyte "b. brasilensis" species. this work assessed the ability of b. kururiensis to endophytically colonize rice plantlets by monitoring different tissues of root-inoculated plants for the presence of bacterial growth in different media, electron microscopy and by 16s rdna analysis. observations of roots, stems and leaves of inocul ... | 2008 | 18797800 |
| hospital outbreak of burkholderia stabilis bacteraemia related to contaminated chlorhexidine in haematological malignancy patients with indwelling catheters. | burkholderia cepacia complex (bcc) is an opportunistic pathogen that occasionally causes hospital outbreaks. this paper describes an outbreak of bcc bacteraemia in haematological malignancy patients related to a contaminated chlorhexidine gluconate solution. eight bcc isolates were obtained from patients hospitalised in the same ward of a cancer centre in a korean hospital. a further three bcc isolates were obtained from 0.5% chlorhexidine gluconate used in the same ward. the isolates were ident ... | 2008 | 18799235 |
| toward modern inhalational bacteriophage therapy: nebulization of bacteriophages of burkholderia cepacia complex. | antibiotic-resistant bacterial infections have renewed interest in finding substitute methods of treatment. the purpose of the present in vitro study was to investigate the possibility of respiratory delivery of a burkholderia cepacia complex (bcc) bacteriophage by nebulized aerosol administration. bacteriophages in isotonic saline were aerosolized with pari lc star and eflow nebulizers, at titers with mean value (standard deviation) of 2.15 x 10(8) (1.63 x 10(8)) plaque-forming unit (pfu)/ml in ... | 2008 | 18800880 |
| gene expression changes linked to antimicrobial resistance, oxidative stress, iron depletion and retained motility are observed when burkholderia cenocepacia grows in cystic fibrosis sputum. | bacteria from the burkholderia cepacia complex (bcc) are the only group of cystic fibrosis (cf) respiratory pathogens that may cause death by an invasive infection known as cepacia syndrome. their large genome (> 7000 genes) and multiple pathways encoding the same putative functions make virulence factor identification difficult in these bacteria. | 2008 | 18801206 |
| effect of antibiotic co-administration on young and mature biofilms of cystic fibrosis clinical isolates: the importance of the biofilm model. | the prognosis of patients with cystic fibrosis (cf) has improved dramatically over the last three decades although the majority of patients still die in early adulthood. infection with pseudomonas aeruginosa has generally been associated with declining lung function and increased mortality in patients. this study aimed to investigate the in vitro activity of tobramycin/clarithromycin combination on biofilms of clinical isolates of p. aeruginosa, meticillin-susceptible and -resistant staphylococc ... | 2009 | 18801647 |
| tuberculosis mimicked by melioidosis. | in regions endemic for tuberculosis (tb) such as india, presumptive anti-tuberculosis treatment is often prescribed. melioidosis, caused by burkholderia pseudomallei, is underdiagnosed in india, due to lack of awareness and a low index of suspicion. | 2008 | 18812053 |
| fluorescence spectroscopy as a promising tool for a polyphasic approach to pseudomonad taxonomy. | fluorescence spectroscopy is an emerging tool for the analysis of biomolecules from complex matrices. we explored the potentialities of the method for the pseudomonad taxonomic purpose at the genus and species level. emission spectra of three intrinsic fluorophores (namely, nadh, tryptophan, and the complex of aromatic amino acids and nucleic acid) were collected from whole bacterial cells. their comparisons were performed through principal component analysis and factorial discriminant analysis. ... | 2009 | 18815829 |
| comparative study of interleukin-1beta expression by peripheral blood mononuclear cells and purified monocytes experimentally infected with burkholderia pseudomallei and burkholderia thailandensis. | burkholderia pseudomallei is a causative agent of melioidosis. the present study investigated il-1beta mrna and protein expression by peripheral blood mononuclear cells and purified monocytes (n = 10) in response to infection with b. pseudomallei and b. thailandensis. similarly increased il-1beta mrna and protein expression was found in both pbmc and purified monocytes stimulated with b. pseudomallei and b. thailandensis. thus, this study suggests that il-1beta response does not differ between i ... | 2008 | 18821217 |
| [phenotypic and genotypic identification of bacteria from the burkholderia cepacia complex]. | evaluation of the diagnostic value of pheno- and genotypic characteristics of b. cepacia strains collection. | 2008 | 18822500 |
| host immunity in the protective response to vaccination with heat-killed burkholderia mallei. | we performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed burkholderia mallei in a susceptible balb/c mouse model of infection. | 2008 | 18823549 |
| a type iv secretion system contributes to intracellular survival and replication of burkholderia cenocepacia. | burkholderia cenocepacia is an important respiratory pathogen in persons with cystic fibrosis (cf). recent studies indicate that b. cenocepacia survives within macrophages and airway epithelial cells in vitro by evading endosome-lysosome fusion. we investigated the role of a plasmid-encoded type iv secretion system in the intracellular survival, replication, and processing of b. cenocepacia. both a wild-type strain (k56-2) and its type iv secretion system mutant (designated lc101) entered and re ... | 2008 | 18824538 |
| burkholderia pseudomallei meningitis following inadequate treatment of melioidotic mycotic aneurysm. | we report a 64-year-old man presenting with meningitis caused by burkholderia pseudomallei predisposed by persistent aortic graft infection following inadequate treatment of a melioidotic mycotic aneurysm. the relapse of melioidosis presenting as acute meningitis is a unique event. successful treatment of deep-seated melioidosis can only be achieved when robust antimicrobial therapy is combined with appropriate surgical debridement. | 2008 | 18830523 |
| chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxb) in rhizobacteria isolated from crop plants. | twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16s rrna analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the beta and gamma subclasses of proteobacteria and actinobacteria. among these, five genera, including dyella, burkholderia, alcaligenes, microbacterium and leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. the thiosulfate-oxidizing dyella, burkholderia, alcaligenes, microbacterium, lei ... | 2008 | 18832027 |
| an antibacterial pyrazole derivative from burkholderia glumae, a bacterial pathogen of rice. | burkholderia glumae, a bacterial pathogen on rice, produced compounds in liquid culture that, in agar diffusion assays, gave strong inhibitory action against erwinia amylovora, the bacterium responsible for fire blight disease of apple and pear trees. products were isolated from culture medium by cation exchange and then purified by bioassay-guided chromatographic methods. two major products were obtained, one of which was not active when fully purified. each product showed a single ninhydrin-st ... | 2008 | 18834606 |
| microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge. | the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ... | 2008 | 18838186 |
| burkholderia pseudomallei infection in a patient with diabetes presenting with multiple splenic abscesses and abscess in the foot: a case report. | abstract: introduction : melioidosis or infection with burkholderia pseudomallei presents with protean manifestations. we present a case of melioidosis in a diabetic patient from india. the case is presented to highlight the importance of early microbiologic diagnosis and subsequent institution of appropriate therapy to achieve a better prognosis case presentation: a male bachelor around 50 years of age from india presented with low grade fever, bilateral ankle swelling and hypochondrial pain. o ... | 2008 | 18840270 |
| bayesian modeling of recombination events in bacterial populations. | we consider the discovery of recombinant segments jointly with their origins within multilocus dna sequences from bacteria representing heterogeneous populations of fairly closely related species. the currently available methods for recombination detection capable of probabilistic characterization of uncertainty have a limited applicability in practice as the number of strains in a data set increases. | 2008 | 18840286 |
| the burkholderia genome database: facilitating flexible queries and comparative analyses. | as the genome sequences of multiple strains of a given bacterial species are obtained, more generalized bacterial genome databases may be complemented by databases that are focused on providing more information geared for a distinct bacterial phylogenetic group and its associated research community. the burkholderia genome database represents a model for such a database, providing a powerful, user-friendly search and comparative analysis interface that contains features not found in other genome ... | 2008 | 18842600 |
| pbad-based shuttle vectors for functional analysis of toxic and highly regulated genes in pseudomonas and burkholderia spp. and other bacteria. | we report the construction of a series of escherichia-pseudomonas broad-host-range expression vectors utilizing the p(bad) promoter and the arac regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads. | 2008 | 18849445 |
| origin and diversity of metabolically active gut bacteria from laboratory-bred larvae of manduca sexta (sphingidae, lepidoptera, insecta). | cultivation-independent analyses based on genetic profiling of partial bacterial 16s rrna genes by pcr-single-strand conformation polymorphism (pcr-sscp), reverse transcriptase (rt)-pcr-sscp of the 16s rrna itself, and stable isotope probing (sip), followed by rt-pcr-sscp, were applied to characterize the diversity of metabolically active bacteria in the larval gut of manduca sexta bred on tobacco leaves under greenhouse conditions. for sip, hatching larvae were fed with leaves from tobacco plan ... | 2008 | 18849461 |
| specific detection of xanthomonas oryzae pv. oryzicola in infected rice plant by use of pcr assay targeting a membrane fusion protein gene. | successful control of xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak, requires a specific and reliable diagnostic tool. a pathovar-specific pcr assay was developed for the rapid and accurate detection of the plant pathogenic bacterium xanthomonas oryzae pv. oryzicola in diseased plant. based on differences in a membrane fusion protein gene of xanthomonas oryzae pv. oryzicola and other microorganisms, which was generated from ncbi (http://www.ncbi.nlm.nih.gov/) and cm ... | 2008 | 18852502 |
| improved cultural selectivity of medically significant fungi by suppression of contaminating bacterial flora employing gallium (iii) nitrate. | incorporation of gallium (iii) nitrate into unsupplemented sabouraud dextrose agar to a final concentration of 512 mg/l (2 mm) suppressed bacterial growth, of the following genera escherichia, enterococcus, klebsiella, listeria, pseudomonas and staphylococcus, in contrast growth of burkholderia cenocepacia, and yeast and filamentous fungi was not affected. supplementation of selective mycological media with gallium (iii) may aid in the selectivity of such media, particularly where clinical speci ... | 2009 | 18854199 |
| sporadic and outbreak cases of melioidosis in southern taiwan: clinical features and antimicrobial susceptibility. | melioidosis has been well known to be endemic in thailand and northern australia, and was reported sporadically in taiwan before 2005. | 2009 | 18854938 |
| cd14 impairs host defense against gram-negative sepsis caused by burkholderia pseudomallei in mice. | cd14 is a pattern-recognition receptor that can facilitate the presentation of bacterial components to either toll-like receptor 2 (tlr2) or tlr4. we have recently shown that during melioidosis, a severe infection caused by the gram-negative bacterium burkholderia pseudomallei, tlr2 but not tlr4 impacts the immune response of the intact host in vivo. | 2008 | 18855560 |
| mycophagous growth of collimonas bacteria in natural soils, impact on fungal biomass turnover and interactions with mycophagous trichoderma fungi. | bacteria of the genus collimonas are widely distributed in soils, although at low densities. in the laboratory, they were shown to be mycophagous, that is, they are able to grow at the expense of living hyphae. however, so far the importance of mycophagy for growth and survival of collimonads in natural soil habitats is unknown. using a collimonas-specific real-time pcr assay, we show here that the invasion of field soils by fungal hyphae (absidia sp.) resulted in a short-term, significant incre ... | 2009 | 18923455 |
| artificial plasmid engineered to simulate multiple biological threat agents. | the objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. we identified and selected specific genes to detect yersinia pestis, francisella tularensis, burkholderia mallei, burkholderia pseudomallei, rickettsia sp., coxiella burnetii, brucella sp., enterohemorrhagic escherichia coli o157:h7, bacillus anthracis, and variola (smallpox) virus. we then designed a ... | 2009 | 18923830 |
| the core and accessory genomes of burkholderia pseudomallei: implications for human melioidosis. | natural isolates of burkholderia pseudomallei (bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. using whole-genome bp microarrays, we examined patterns of gene presence and absence across 94 south east asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the bp k96243 reference genome was common to all the strains representing the bp "core genome", comprising genes largely ... | 2008 | 18927621 |
| dgge with genomic dna: suitable for detection of numerically important organisms but not for identification of the most abundant organisms. | identification of all important community members as well as of the numerically dominant members of a community are key aspects of microbial community analysis of bioreactor samples. a systematic study was conducted with artificial consortia to test whether denaturing gradient gel electrophoresis (dgge) is a reliable technique to obtain such community data under conditions where results would not be affected by differences in dna extraction efficiency from cells. a total of 27 consortia were est ... | 2008 | 18929384 |
| effects of inoculum, delayed loading, and storage temperature of blood culture bottles on the detection of burkholderia pseudomallei. | detection of burkholderia pseudomallei in blood culture is critical to prompt appropriate treatment. various factors may affect culture positivity. this study evaluated effects of various inocula (0.2-100 cfu/ml), delayed bottle loading periods (12-48 h), and storage temperatures (ambient, 4 and 35 degrees c) for the detection of b. pseudomallei by the bact/alert system. the results suggested that bottles may be preincubated at 35 degrees c for up to 12 h, or held at ambient temperature for up t ... | 2008 | 18929457 |
| risa-hplc analysis of lung bacterial colonizers of cystic fibrosis children. | microbiological analysis of sputum samples, from children affected by cystic fibrosis (cf) and showing signs of acute or chronic infections, is routinely performed by culture-dependent approaches involving selective media and biochemical tests. these identification schemes are time-consuming, and may lead to false negative results. the aim of this work was to evaluate the efficacy of a ribosomal intergenic spacer analysis (risa) coupled to high performance liquid chromatography (hplc) for the de ... | 2009 | 18929602 |
| the genome of burkholderia cenocepacia j2315, an epidemic pathogen of cystic fibrosis patients. | bacterial infections of the lungs of cystic fibrosis (cf) patients cause major complications in the treatment of this common genetic disease. burkholderia cenocepacia infection is particularly problematic since this organism has high levels of antibiotic resistance, making it difficult to eradicate; the resulting chronic infections are associated with severe declines in lung function and increased mortality rates. b. cenocepacia strain j2315 was isolated from a cf patient and is a member of the ... | 2009 | 18931103 |
| mycotic aneurysm in northeast thailand: the importance of burkholderia pseudomallei as a causative pathogen. | forty cases of mycotic aneurysm that occurred during the period 1993-2007 were reviewed. the most common causative pathogen was burkholderia pseudomallei (17 cases; 42.5%). postoperative complications and bacteremia were significantly more common among patients with mycotic aneurysm due to b. pseudomallei than they were among patients with mycotic aneurysm that was not attributable to b. pseudomallei. in a region in which melioidosis is endemic, empirical antimicrobial therapy for suspected myco ... | 2008 | 18937581 |
| in silico microarray probe design for diagnosis of multiple pathogens. | with multiple strains of various pathogens being sequenced, it is necessary to develop high-throughput methods that can simultaneously process multiple bacterial or viral genomes to find common fingerprints as well as fingerprints that are unique to each individual genome. we present algorithmic enhancements to an existing single-genome pipeline that allows for efficient design of microarray probes common to groups of target genomes. the enhanced pipeline takes advantage of the similarities in t ... | 2008 | 18940003 |
| bacterial populations associated with rice seed in the tropical environment. | abstract during the 1995 wet season, harvested rice seed was collected from farmers' fields at different locations in iloilo, philippines. bacterial isolations from crushed seed yielded 428 isolates. the isolates were characterized by box-polymerase chain reaction fingerprinting of total genomic dna and represented 151 fingerprint types (fpt). most fpts were found on a single occasion, although matching fingerprints for isolates from different samples also were found. identifications were made b ... | 2001 | 18943348 |
| postinfection biological control of oomycete pathogens of pea by burkholderia cepacia ammdr1. | abstract burkholderia cepacia ammdr1 is a biocontrol agent that reduces pythium damping-off and aphanomyces root rot severity on peas in the field. we studied the effect of b. cepacia ammdr1 on post-infection stages in the life cycles of these pathogens, including mycelial colonization of the host, production of oogonia, and production of secondary zoospore inoculum. we used burkholderia cepacia 1324, a seed and rootcolonizing but antibiosis-deficient tn5 mutant of b. cepacia ammdr1, to study me ... | 2001 | 18943851 |