| edwardsiella tarda bacteremia. a rare but fatal water- and foodborne infection: review of the literature and clinical cases from a single centre. | edwardsiella tarda bacteremia (etb) can be a fatal disease in humans. | 2017 | 26744588 |
| expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from vibrio vulnificus. | undecaprenyl pyrophosphate phosphatase (uppp), a cell membrane integral enzyme, catalyzes the dephosphorylation of undecaprenyl pyrophosphate to undecaprenyl phosphate, which is an essential carrier lipid in bacterial cell wall synthesis. we previously purified e. coli uppp and concluded that its catalytic site is likely located in the periplasm. to search for additional natural uppp homologs to elucidate what constitutes a common catalytic mechanism and to gain a better chance of obtaining high ... | 2017 | 28302513 |
| plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters. | periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ... | 2017 | 28235124 |
| development of a regulatable expression system for the functional study of vibrio vulnificus essential genes. | we developed a regulatable gene expression system for vibrio vulnificus, which contains a laciq-ptrc cassette. monomeric red fluorescence protein (mrfp) was used as a reporter to test this system. the results showed that this system tightly controlled the expression of mrfp without leaky expression and was suitable for the controlled expression of genes encoding recombinant proteins in v. vulnificus. to demonstrate the utility of this system, a dominant negative form of v. vulnificus vvmo6_rs049 ... | 2017 | 28044234 |
| a c-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish, sebastes schlegelii. | c-type lectins (ctls) are important pattern recognition receptors (prrs) that play vital roles in innate immunity. in teleosts, a number of ctls have been reported, but their in vivo effects on host defense are still limited. in this study, a ctl homolog (sslec1) was identified from black rockfish, sebastes schlegelii, and its structure, expression and biological function was analyzed. the open reading frame of sslec1 is 633 bp, with a 5'- untranslated region (utr) of 36 bp and a 3'- utr of 117 ... | 2016 | 27569982 |
| functional analysis of vibrio vulnificus orthologs of escherichia coli rraa and rnase e. | rnase e plays an important role in the degradation and processing of rna in escherichia coli. the enzymatic activity of rnase e is controlled by the protein inhibitors rraa and rrab. the marine pathogenic bacterium vibrio vulnificus also contains homologs of rnase e and rraa, designated as rnase ev, rraav1, and rraav2. here, we report that rraav1 actively inhibits the enzymatic activity of rnase ev in vivo and in vitro by interacting with the c-terminal domain of rnase ev. coexpression of rraav1 ... | 2016 | 26888524 |
| vibrio spp. from macrobrachium amazonicum prawn farming are inhibited by moringa oleifera extracts. | to investigate the in vitro antimicrobial potential of extracts of stem, leaves, flowers, pods and seeds of moringa oleifera (m. oleifera) against vibrio spp. from hatchery water and the prawn macrobrachium amazonicum. | 2015 | 26614991 |
| ttrap is a critical factor in grouper immune response to virus infection. | ttrap (traf and tnf receptor-associated protein) is latest identified cytosolic protein that serves as a negative regulator for tnf signaling pathway. in this study, a member of tnf superfamily, ttrap gene (designed as ecttrap) was cloned from grouper, epinephelus coioides. there was an exo_endo_phos type domain in ecttrap, and it was well conserved when compared with other ttraps, especially the endonuclease activity related motifs. ecttrap exhibited prominent endonuclease activity against the ... | 2015 | 26172204 |
| vibrio vulnificus glycogen branching enzyme preferentially transfers very short chains: n1 domain determines the chain length transferred. | the glycogen branching enzyme from vibrio vulnificus (vvgbe) transfers short side chains (dp 3-5) significantly greater than any other bacterial glycogen branching enzyme (gbe). to elucidate the role of the n-domain of vvgbe in the unique branching pattern, domain-truncated (n1 and n) and n1-domain-swapped (with vvgbe n1 replacing the counter part of escherichia coli gbe) mutants were constructed. the truncation mutants synthesized branched products with a greatly reduced proportion of short cha ... | 2015 | 25819440 |
| hygiene and health risks associated with the consumption of edible lamellibranch molluscs. | edible lamellibranch molluscs are frequently the cause of very serious outbreaks of food poisoning. microbiological suitability for human consumption, according to ec reg. n. 1441/2007, is only based on two faecal bacteriological contamination parameters (escherichia coli and salmonella) and does not consider other microorganisms which are naturally present within the marine environment and are potentially pathogenic such as those belonging to the genus vibrio. in this study, a total of 540 samp ... | 2015 | 25744696 |
| functional analysis of vibrio vulnificus rnd efflux pumps homologous to vibrio cholerae vexab and vexcd, and to escherichia coli acrab. | resistance-nodulation-division (rnd) efflux pumps are associated with multidrug resistance in many gram-negative pathogens. the genome of vibrio vulnificus encodes 11 putative rnd pumps homologous to those of vibrio cholerae and escherichia coli. in this study, we analyzed three putative rnd efflux pumps, showing homology to v. cholerae vexab and vexcd and to e. coli acrab, for their functional roles in multidrug resistance of v. vulnificus. deletion of the vexab homolog resulted in increased su ... | 2015 | 25740377 |
| histidine phosphocarrier protein regulates pyruvate kinase a activity in response to glucose in vibrio vulnificus. | the bacterial phosphoenolpyruvate:sugar phosphotransferase system (pts) consists of two general energy-coupling proteins [enzyme i and histidine phosphocarrier protein (hpr)] and several sugar-specific enzyme iis. although, in addition to the phosphorylation-coupled transport of sugars, various regulatory roles of pts components have been identified in escherichia coli, much less is known about the pts in the opportunistic human pathogen vibrio vulnificus. in this study, we have identified pyruv ... | 2015 | 25598011 |
| efficacy of neutral electrolyzed water for reducing pathogenic bacteria contaminating shrimp. | pathogenic contamination is a food safety concern. this study was conducted to investigate the efficacy of neutral electrolyzed water (new) in killing pathogens, namely, vibrio parahaemolyticus, vibrio vulnificus, salmonella enteritidis, and escherichia coli in shrimp. pure cultures of each pathogen were submerged separately in new containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. for each concentration, three submersion times were tested: 1, 3, and 5 min. the popul ... | 2014 | 25474069 |
| characterization of the vibrio vulnificus 1-cys peroxiredoxin prx3 and regulation of its expression by the fe-s cluster regulator iscr in response to oxidative stress and iron starvation. | peroxiredoxins (prxs) are ubiquitous antioxidant enzymes that reduce toxic peroxides. a new vibrio vulnificus prx, named prx3, was identified and characterized in this study. biochemical and mutational analyses revealed that prx3 reduces h2o2, utilizing glutaredoxin 3 (grx3) and glutathione (gsh) as reductants, and requires only n-terminal peroxidatic cysteine for its catalysis. these results, combined with the monomeric size of prx3 observed under non-reducing conditions, suggested that prx3 is ... | 2014 | 25398878 |
| distinct characteristics of oxyr2, a new oxyr-type regulator, ensuring expression of peroxiredoxin 2 detoxifying low levels of hydrogen peroxide in vibrio vulnificus. | two peroxiredoxins, prx1 and prx2, were previously identified in vibrio vulnificus. besides oxyr1, a homologue of escherichia coli oxyr (ecoxyr), oxyr2 that shares low homology with ecoxyr was first identified in v. vulnificus. oxyr2 activated prx2 during aerobic growth, while oxyr1 activated prx1 only when exposed to exogenous h2o2. oxyr2 was oxidized to form a reversible c206 to c215 disulphide bond by sensing low levels of h2o2, which were insufficient to oxidize oxyr1, and only the oxidized ... | 2014 | 25041181 |
| vibrio vulnificus mo6-24/o lipopolysaccharide stimulates superoxide anion, thromboxane b₂, matrix metalloproteinase-9, cytokine and chemokine release by rat brain microglia in vitro. | although human exposure to gram-negative vibrio vulnificus (v. vulnificus) lipopolysaccharide (lps) has been reported to result in septic shock, its impact on the central nervous system's innate immunity remains undetermined. the purpose of this study was to determine whether v. vulnificus mo6-24/o lps might activate rat microglia in vitro and stimulate the release of superoxide anion (o₂⁻), a reactive oxygen species known to cause oxidative stress and neuronal injury in vivo. brain microglia we ... | 2014 | 24675728 |
| functional analysis of tolc homologs in vibrio vulnificus. | gram-negative bacteria use tripartite pumps to transport antibacterial drugs and other toxic compounds across the inner and outer membranes, which are separated by the periplasmic space. the tolc protein is an outer membrane factor that participates in the formation of tripartite efflux pumps. the genome of vibrio vulnificus encodes two e. coli tolc homologs, tolcv1 and tolcv2. here, we show that both tolcv1 and tolcv2 are involved in the efflux of antimicrobial agents. deletion of tolcv1 result ... | 2014 | 24515351 |
| interaction between the α-barrel tip of vibrio vulnificus tolc homologs and acra implies the adapter bridging model. | the acrab-tolc multidrug efflux pump confers resistance to escherichia coli against many antibiotics and toxic compounds. the tolc protein is an outer membrane factor that participates in the formation of type i secretion systems. the genome of vibrio vulnificus encodes two proteins homologous to the e. coli tolc, designated tolcv1 and tolcv2. here, we show that both tolcv1 and tolcv2 partially complement the e. coli tolc function and physically interact with the membrane fusion protein acra, a ... | 2014 | 24500479 |
| pvv3, a new shuttle vector for gene expression in vibrio vulnificus. | an efficient electroporation procedure for vibrio vulnificus was designed using the new cloning vector pvv3 (3,107 bp). transformation efficiencies up to 2 × 10(6) transformants per μg dna were achieved. the vector stably replicated in both v. vulnificus and escherichia coli and was also successfully introduced into vibrio parahaemolyticus and vibrio cholerae. to demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (gfp) gene and the vvhba hemolysin oper ... | 2014 | 24362421 |
| peroxidase and photoprotective activities of magnesium protoporphyrin ix. | magnesium-protoporphyrin ix (mg-ppn), which is formed through chelation of protoporphyrin ix (ppn) with mg ion by mg chelatase, is the first intermediate for the (bacterio)chlorophyll biosynthetic pathway. interestingly, mg-ppn provides peroxidase activity (approximately 4 × 10(-2) units/micrometer) detoxifying h2o2 in the presence of electron donor(s). the peroxidase activity was not detected unless ppn was chelated with mg ion. mg-ppn was found freely diffusible through the membrane of escheri ... | 2014 | 24317481 |
| functional conservation of rnase iii-like enzymes: studies on a vibrio vulnificus ortholog of escherichia coli rnase iii. | bacterial ribonuclease iii (rnase iii) belongs to the rnase iii enzyme family, which plays a pivotal role in controlling mrna stability and rna processing in both prokaryotes and eukaryotes. in the vibrio vulnificus genome, one open reading frame encodes a protein homologous to e. coli rnase iii, designated vv-rnase iii, which has 77.9 % amino acid identity to e. coli rnase iii. here, we report that vv-rnase iii has the same cleavage specificity as e. coli rnase iii in vivo and in vitro. express ... | 2014 | 24241331 |
| identification of 62-kda protein as an immunogenic antigen of vibrio vulnificus for humans. | vibrio vulnificus infection can cause necrotizing fasciitis and sepsis and can develop within a few days despite intensive care. the mortality rate is up to 60% in vulnerable people. most patients infected with this microbe have chronic liver disease, especially liver cirrhosis or cancer, as an underlying disease. v. vulnificus infection is opportunistic, and there is an urgent need to develop an anti- v. vulnificus vaccine. thus, it is important to identify immunogenic antigens. we collected hu ... | 2013 | 24040694 |
| ubiquitin reference technique and its use in ubiquitin-lacking prokaryotes. | in a pulse-chase assay, the in vivo degradation of a protein is measured through a brief labeling of cells with, for example, a radioactive amino acid, followed by cessation of labeling and analysis of cell extracts prepared at different times afterward ("chase"), using immunoprecipitation, electrophoresis and autoradiography of a labeled protein of interest. a conventional pulse-chase assay is fraught with sources of data scatter, as the efficacy of labeling and immunoprecipitation can vary, an ... | 2013 | 23825692 |
| live and dead gfp-tagged bacteria showed indistinguishable fluorescence in caenorhabditis elegans gut. | caenorhabditis elegans has been used for studying host-pathogen interactions since long, and many virulence genes of pathogens have been successfully identified. in several studies, fluorescent pathogens were fed to c. elegans and fluorescence observed in the gut was considered an indicator for bacterial colonization. however, the grinder in the pharynx of these nematodes supposedly crushes the bacterial cells, and the ground material is delivered to the intestine for nutrient absorption. theref ... | 2013 | 23812817 |
| the α-barrel tip region of escherichia coli tolc homologs of vibrio vulnificus interacts with the maca protein to form the functional macrolide-specific efflux pump macab-tolc. | tolc and its homologous family of proteins are outer membrane factors that are essential for exporting small molecules and toxins across the outer membrane in gram-negative bacteria. two open reading frames in the vibrio vulnificus genome that encode proteins homologous to escherichia coli tolc, designated tolcv1 and tolcv2, have 51.3% and 29.6% amino acid identity to tolc, respectively. in this study, we show that tolcv1 and tolcv2 functionally and physically interacted with the membrane fusion ... | 2013 | 23625214 |
| characterization of complement 1q binding protein of tiger shrimp, penaeus monodon, and its c1q binding activity. | the receptor for the globular heads of c1q, c1qbp/gc1qr/p33, is a multicompartmental, multifunctional cellular protein with an important role in infection and in inflammation. in the present study, we identified and characterized the complement component 1q subcomponent binding protein (c1qbp) from the tiger shrimp penaeus monodon (designated as pmc1qbp). the open reading frame of pmc1qbp encodes 262 amino acid residues with a conserved mam33 domain, an arginine-glycine-aspartate cell adhesion m ... | 2013 | 23085472 |
| identification of the vibrio vulnificus htpg gene and its influence on cold shock recovery. | an htpg gene encoding the heat shock protein htpg was identified and cloned from vibrio vulnificus. the deduced amino acid sequence of htpg from v. vulnificus exhibited 71 and 85% identity to those reported from escherichia coli and v. cholera, respectively. functions of htpg were assessed by the construction of an isogenic mutant whose htpg gene was deleted and by evaluating its phenotype changes during and after cold shock. the results demonstrated that recovery of the wild type from cold shoc ... | 2012 | 22923124 |
| oral administration of recombinant epinecidin-1 protected grouper (epinephelus coioides) and zebrafish (danio rerio) from vibrio vulnificus infection and enhanced immune-related gene expressions. | immunostimulatory effects of the oral administration of the recombinant epinecidin-1 protein from bl21 escherichia coli (containing the pet28a-epinecidin-1-dsred plasmid) were studied in grouper (epinephelus coioides) and zebrafish (danio rerio). for this purpose, fish were fed diets for 30 days containing the recombinant epinecidin-1 protein from bl21 e. coli (containing the pet28a-epinecidin-1-dsred plasmid) at different bacterial numbers (10(4), 10(6), 10(8), and 10(10) colony-forming units ( ... | 2012 | 22554570 |
| cyclic amp-receptor protein activates aerobactin receptor iuta expression in vibrio vulnificus. | the ferrophilic bacterium vibrio vulnificus can utilize the siderophore aerobactin of escherichia coli for iron acquisition via its specific receptor iuta. this siderophore piracy by v. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. in this study, we examined the effects of glucose, cyclic amp (camp), and camp-receptor protein (crp) on iuta expression in v. vulnificus. glucose dose-dependently repressed iuta expression. a mutation in cya ... | 2012 | 22538662 |
| crystallization and preliminary x-ray diffraction analysis of a novel wild-type blue fluorescent protein from vibrio vulnificus ckm-1. | the use of green fluorescent protein (gfp) for non-invasive in vivo imaging is limited to aerobic systems, as chromophore formation requires oxygen. however, a novel nadph-dependent blue fluorescent protein from vibrio vulnificus ckm-1 (bfpvv) that emits blue fluorescence in both aerobic and anaerobic systems has recently been discovered. wild-type bfpvv was overexpressed in escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. the resulting bfpvv crystals d ... | 2012 | 22505417 |
| hydrogen peroxide causes vibrio vulnificus bacteriolysis accelerated by sulfonyl fluoride compounds. | induction of bacteriolysis of vibrio vulnificus cells by 10 mm hydrogen peroxide (h(2)o(2)) was analyzed. all vibrio species examined, except for vibrio hollisae, were lysed by 10 mm h(2)o(2). bacteriophage induction was not the cause of h(2)o(2)-induced bacteriolysis. autolysis is also known to cause bacteriolysis. vvps protein is a serine protease of v. vulnificus essential for autolysis. vvps mutant underwent h(2)o(2)-induced bacteriolysis in the same manner as the wild type. protease inhibit ... | 2015 | 26316164 |
| vibrio vulnificus necrotizing fasciitis preceding herpes zoster. | a 74-year-old white man presented with unilateral radicular pain extending across the left side of his chest and back. a diagnosis of postherpetic neuralgia, a sequela of herpes zoster, was made. herpes zoster represents a reactivation of the varicella zoster virus that lies dormant in patients with past chickenpox. risk factors for the disease include advanced age, stress, immunodeficiency, and immunosuppression. treatment of herpes zoster entails traditional antiviral medications, while preven ... | 2013 | 23382617 |
| intranasal immunization with a flagellin-adjuvanted peptide anticancer vaccine prevents tumor development by enhancing specific cytotoxic t lymphocyte response in a mouse model. | human papillomavirus (hpv) is a significant cause of cervical cancer-related deaths worldwide. because hpv is a sexually transmitted mucosal pathogen, enhancement of antigen-specific mucosal immune response likely serves good strategy for vaccination. however, mucosal vaccines generally do not induce strong enough immune responses. previously we proved that a bacterial flagellin, vibrio vulnificus flab, induce strong antigen-specific immune responses by stimulating the toll-like receptor 5. in t ... | 2013 | 23858404 |
| flagellin enhances tumor-specific cd8⁺ t cell immune responses through tlr5 stimulation in a therapeutic cancer vaccine model. | tumor antigen (ta)-specific immunotherapy is an emerging approach for cancer treatment. potent adjuvants are prerequisites to the immunotherapy for overcoming the low immunogenicity of tas. we previously demonstrated that a bacterial flagellin, vibrio vulnificus flab, has potent adjuvant activity in various vaccination models. in this study, we investigated whether the flab protein could be a potent adjuvant for a human papillomavirus 16 e6 and e7 (e6/e7) peptide-based anticancer immunotherapy. ... | 2013 | 23831323 |
| tc38, a teleost tfpi-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids. | tissue factor pathway inhibitor 2 (tfpi-2) is an analog of tfpi-1 and a potent endogenous inhibitor of tissue factor (tf)-mediated blood coagulation. recent reports have proven that the c-terminal of tfpi-2 peptides in humans and several other vertebrates possesses antibacterial activity against gram-positive and gram-negative bacteria. in our previous study, we reported that the tfpi-2 peptide, tc38 in tongue sole (cynoglossus semilaevis) was active against micrococcus luteus. in this study, we ... | 2017 | 28263880 |
| immunological evaluation of vibrio alginolyticus, vibrio harveyi, vibrio vulnificus and infectious spleen and kidney necrosis virus (isknv) combined-vaccine efficacy in epinephelus coioides. | combined vaccines are immunological products intended for immunization against multifactorial infectious diseases caused by different types or variants of pathogens. in this study, the effectiveness of vibrio alginolyticus (va), vibrio harveyi (vh), vibrio vulnificus (vv) and infectious spleen and kidney necrosis virus (isknv), an iridovirus, combined-vaccine (vibrio and isknv combined vaccines, vicv), va+vh+vv inactive vaccine (viv) and isknv whole cell inactive vaccine (iwciv) in epinephelus c ... | 2012 | 23010220 |
| necrotizing fasciitis and sepsis caused by vibrio vulnificus and klebsiella pneumoniae in diabetic patients. | vibrio vulnificus related necrotizing fasciitis is a fatal, rapidly progressive soft-tissue infection. necrotizing fasciitis caused by klebsiella pneumoniae is rare, which is indistinguishable from v. vulnificus infection in the emergency room. the purpose of this study was to compare the clinical characteristics and outcome between these two pathogens in diabetic patients. | 2016 | 25179718 |
| rt-pcr-dgge analysis to elucidate the dominant bacterial species of industrial spanish-style green table olive fermentations. | this paper describes the dominant bacterial species metabolically active through the industrial production of spanish-style manzanilla and gordal olives. for this purpose, samples (brines and fruits) obtained at 0, 15, and 90 fermentation days were analyzed by a culture-independent approach to determine viable cells by reverse transcription of rna and further pcr-dgge analysis, detecting at least 7 different species. vibrio vulnificus, lactobacillus plantarum group, and lactobacillus parafarragi ... | 2016 | 27582739 |
| a tfpi-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, cynoglossus semilaevis. | tissue factor pathway inhibitor 1 (tfpi-1) is a serine protease inhibitor that inhibits tissue factor (tf)-mediated coagulation. the c-terminal region of tfpi-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. in a previous study, a c-terminal peptide, tc24 (with 24 amino acids), derived from tongue sole (cynoglossus semilaevis) tfpi-1, was synthesized and found antibacterial against micrococcus luteus. in the present study, the antibacterial spectru ... | 2017 | 27840169 |
| development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals. | rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ... | 2014 | 24954661 |
| the membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution. | membrane localization domain (mld) was first proposed for a 4-helix-bundle motif in the crystal structure of the c1 domain of pasteurella multocida toxin (pmt). this structure motif is also found in the crystal structures of several clostridial glycosylating toxins (tcda, tcdb, tcsl, and tcna). the ras/rap1-specific endopeptidase (rrsp) module of the multifunctional autoprocessing repeats-in-toxins (martx) toxin produced by vibrio vulnificus has sequence homology to the c1-c2 domains of pmt, inc ... | 2017 | 27977897 |
| backbone and side-chain assignments of an effector membrane localization domain from vibrio vulnificus martx toxin. | (1)h, (13)c, and (15)n chemical shift assignments are presented for the isolated four-helical bundle membrane localization domain from the domain of unknown function 5 (duf5) effector (mld(vvduf5)) of the martx toxin from vibrio vulnificus in its solution state. we have assigned 97% of all backbone and side-chain carbon atoms, including 96% of all backbone residues. secondary chemical shift analysis using talos+ demonstrates four helices that align with those predicted by structure homology mode ... | 2014 | 23765285 |
| mechanisms for pseudoalteromonas piscicida-induced killing of vibrios and other bacterial pathogens. | pseudoalteromonas piscicida are gram-negative gammaproteobacteria found in the marine environment. three strains of pigmented p. piscicida were isolated from seawater and partially characterized by inhibition studies, electron microscopy, and proteolytic enzyme production. growth inhibition and death occurred around colonies of p. piscicida on lawns of the naturally-occurring marine pathogens vibrio vulnificus, v. parahaemolyticus, v. cholerae, photobacterium damselae and shewanella algae inhibi ... | 2017 | 28363962 |
| novel host-specific iron acquisition system in the zoonotic pathogen vibrio vulnificus. | vibrio vulnificus is a marine bacterium associated with human and fish (mainly farmed eels) diseases globally known as vibriosis. the ability to infect and overcome eel innate immunity relies on a virulence plasmid (pvvbt2) specific for biotype 2 (bt2) strains. in the present study, we demonstrated that pvvbt2 encodes a host-specific iron acquisition system that depends on an outer membrane receptor for eel transferrin called vep20. the inactivation of vep20 did not affect either bacterial growt ... | 2015 | 25630302 |
| predominant bacteria associated with red snapper from the northern gulf of mexico. | since the deepwater horizon oil spill in 2010, anecdotal observations of red snapper lutjanus campechanus from the northern gulf of mexico exhibiting unusual external lesions have been reported. two opportunistic bacterial fish pathogens, vibrio vulnificus and photobacterium damselae, were recovered from the fish and were deemed responsible for the abnormalities. however, the culturable microbiota of healthy red snapper has not yet been characterized. we analyzed the heterotrophic bacteria assoc ... | 2013 | 24341770 |
| nmr study of the o-specific polysaccharide and the core oligosaccharide from the lipopolysaccharide produced by plesiomonas shigelloides o24:h8 (strain cnctc 92/89). | the structures of the o-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from plesiomonas shigelloides o24:h8, strain cnctc 92/89, have been investigated by nmr spectroscopy and esi mass spectrometry. the o-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [→3)-α-fucpnac-(1→3)-α-galpnaca-(1→3)-α-quipnac-(1→] and of α-rhapnac (1→4) linked to the galpnaca residue. an identical structure has been reported for the capsular p ... | 2015 | 25834986 |
| zebrafish fed on recombinant artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon vibrio vulnificus infection. | artemia has been used extensively in aquaculture as fodder for larval fish, shrimp, and shellfish. epinecidin-1, an antimicrobial peptide, was isolated from grouper (epinephelus coioides) in 2005. epinecidin-1 has been previously reported to possess antimicrobial activity against several gram-positive and gram-negative bacterial species, including staphylococcus coagulase, pseudomonas aeruginosa, streptococcus pyogenes, and vibrio vulnificus. in this study, we used electroporation to introduce p ... | 2015 | 25462461 |
| necrotizing soft tissue infection with gas formation caused by vibrio vulnificus and misdiagnosed as pseudomonas aeruginosa. | | 2013 | 23158593 |
| characterization of prohibitin 1 as a host partner of vibrio vulnificus rtxa1 toxin. | rtxa1 toxin, which results in cytoskeletal rearrangement, contact cytotoxicity, hemolysis, tissue invasion, and lethality in mice, is the most potent cytotoxic virulence factor of vibrio vulnificus. bioinformatics analysis of rtxa1 predicted 4 functional domains that presumably performed discrete functions during host cell killing. v. vulnificus rtxa1 has a unique domain designated as rtxa1-d2, corresponding to amino acids 1951-2574, which is absent in vibrio cholerae multifunctional-autoprocess ... | 2016 | 26136468 |
| molecular cloning, characterization of one key molecule of teleost innate immunity from orange-spotted grouper (epinephelus coioides): serum amyloid a. | the orange-spotted grouper (epinephelus coioides), a favorite marine food fish, is widely cultured in china and southeast asian countries. however, little is known about its acute phase response (apr) caused by viral diseases. serum amyloid a (saa) is a major acute phase protein (app). in this study, a new saa homologous (ecsaa) gene was cloned from grouper, e. coioides, by rapid amplification of cdna ends (race) pcr. the full-length cdna sequence of saa was 508 bp and contained a 363 bp open re ... | 2013 | 23178260 |
| two new benzoate derivatives and one new phenylacetate derivative from a marine-derived fungus engyodontium album. | two new benzoate derivatives, ethyl 3,5-dimethoxy-2-propionylbenzoate (1) and ethyl 3,5-dihydroxy-2-propionylbenzoate (2), and one new phenylacetate derivative, ethyl 3,5-dimethoxy-2-propionylphenylacetate (3), together with 9 known compounds, were isolated from the fermentation of engyodontium album derived from deep sea sediment. their structures were elucidated by spectroscopic techniques, such as nmr, ir and hresims. compound 3 exhibited inhibitory activities against methicillin-resistant st ... | 2017 | 27750436 |
| identification and expression analysis of dicer2 in black tiger shrimp (penaeus monodon) responses to immune challenges. | dicer is a key initiative protein of the rna interference (rnai) pathway that produces small interfering rnas (sirnas) or micro rnas (mirna), which then leads to rna-directed gene regulation or viral immunity. in the present study, we identified and characterized a dicer2 cdna from black tiger shrimp penaeus monodon (designated as pmdcr2). the full length cdna of pmdcr2 contains a 5' untranslated region (utr) of 109 bp, an open reading frame (orf) of 4509 bp and a 3' utr of 842 bp. the molecular ... | 2013 | 23583958 |
| isolation and characterization of homologous trbp cdna for rna interference in penaeus monodon. | the transactivation response rna-binding protein (trbp) interacts with dicer and binds to double-stranded rna as a critical component of the rna-induced silencing complex, which is a key complex in the rna interference pathway. the full-length cdna of trbp from the tiger prawn, penaeus monodon, (pmtrbp; 1548 bp long with a 1029 bp coding region) was isolated. the encoded polypeptide of 343 amino acids had a predicted molecular mass of 36.8 kda. sequence homology and phylogenetic analysis indicat ... | 2013 | 23207479 |
| autopsy cases of fulminant bacterial infection in adults: clinical onset depends on the virulence of bacteria and patient immune status. | to assist physicians in recognizing the potentially fatal onset of symptoms in cases of fulminant bacterial infection, we analyzed 11 autopsy cases of such infection (four caused by streptococcus pneumoniae, four by s. pyogenes, one by s. dysgalactiae subsp. equisimilis, one by staphylococcus aureus, and one by vibrio vulnificus). clinicohistopathologic features were evaluated. all patients experienced sudden onset of hypotension and multiple organ failure, leading to unexpected death. blood cul ... | 2012 | 22350403 |
| biotreatment of petrochemical wastewater: a case study from northern tunisia. | a full-scale study has been conducted to assess the bioaugmentation efficiency of trickling filter process to treat petrochemical wastewater from a lubricant industry recycling waste oils. during 45 weeks, the organic loading rate (olr) in the trickling filter was increased stepwise from 0.9 to 4 kg of chemical oxygen demand (cod)/(m3·day) at the end of the upgrading period as the flow rate (fr) reached the value of 30 m3/day. the removal, obtained in terms of percentage, for cod ranged from 60 ... | 2017 | 27094680 |
| electrotransfer of the tilapia piscidin 3 and tilapia piscidin 4 genes into skeletal muscle enhances the antibacterial and immunomodulatory functions of oreochromis niloticus. | tilapia piscidin 3 (tp3) and tilapia piscidin 4 (tp4) are antimicrobial peptides recently isolated from oreochromis niloticus. we previously showed that synthetic tp3 and tp4 possessed antimicrobial activities. here, we analyzed the bactericidal abilities and immunomodulatory properties of these amps following the electroporation of pcmv-gfp-tp3 or pcmv-gfp-tp4 plasmid into tilapia (o. niloticus) muscle and subsequent infection with vibrio vulnificus or streptococcus agalactiae. prior overexpres ... | 2016 | 26828260 |
| characterization of tilapia (oreochromis niloticus) viperin expression, and inhibition of bacterial growth and modulation of immune-related gene expression by electrotransfer of viperin dna into zebrafish muscle. | viperin is an anti-viral protein, induced by viral infection. in this study, we examined whether over-expression of viperin in fish muscle could inhibit bacterial growth. we first obtained the cdna sequence of tilapia viperin, through rt-pcr-mediated cloning and sequencing. the cdna sequence was similar to those of several fish viperins in genbank, and it was predicted to encode the conserved domain of radical s-adenosylmethionine superfamily proteins. phylogenetic analysis revealed that tilapia ... | 2013 | 23237906 |
| five different piscidins from nile tilapia, oreochromis niloticus: analysis of their expressions and biological functions. | piscidins are antimicrobial peptides (amps) that play important roles in helping fish resist pathogenic infections. through comparisons of tilapia est clones, the coding sequences of five piscidin-like amps (named tp1∼5) of nile tilapia, oreochromis niloticus, were determined. the complete piscidin coding sequences of tp1, -2, -3, -4, and -5 were respectively composed of 207, 234, 231, 270, and 195 bases, and each contained a translated region of 68, 77, 76, 89, and 64 amino acids. the tissue-sp ... | 2012 | 23226256 |
| responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus. | marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases. | 2017 | 28390964 |
| season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea. | vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ... | 2017 | 27836844 |
| prevalence and antimicrobial resistance of vibrio spp. in retail and farm shrimps in ecuador. | the aim of this study was to investigate the prevalence of vibrio spp. in shrimp at retail and in shrimp farms in ecuador and to determine the antimicrobial agent resistance patterns of farm isolates. the presence of genes linked to early mortality syndrome (ems) or acute hepatopancreatic necrosis disease (ahpnd) also was evaluated. vibrio spp. were isolated from retail shrimps in cuenca, ecuador, and farm shrimps originating from provinces el oro and guayas, ecuador. a total of 229 shrimp sampl ... | 2015 | 26555534 |
| deep-sea hydrothermal vent bacteria related to human pathogenic vibrio species. | vibrio species are both ubiquitous and abundant in marine coastal waters, estuaries, ocean sediment, and aquaculture settings worldwide. we report here the isolation, characterization, and genome sequence of a novel vibrio species, vibrio antiquarius, isolated from a mesophilic bacterial community associated with hydrothermal vents located along the east pacific rise, near the southwest coast of mexico. genomic and phenotypic analysis revealed v. antiquarius is closely related to pathogenic vibr ... | 2015 | 25964331 |
| occurrence of virulence genes among vibrio cholerae and vibrio parahaemolyticus strains from treated wastewaters. | pathogenic vibrio species are an important cause of foodborne illnesses. the aim of this study was to describe the occurrence of potentially pathogenic vibrio species in the final effluents of a wastewater treatment plant and the risk that they may pose to public health. during the 1-year monitoring, a total of 43 vibrio strains were isolated: 23 vibrio alginolyticus, 1 vibrio cholerae, 4 vibrio vulnificus, and 15 vibrio parahaemolyticus. the pcr investigation of v. parahaemolyticus and v. chole ... | 2014 | 25023745 |
| multiplex pcr assays for the detection of vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae with an internal amplification control. | a multiplex polymerase chain reaction (pcr) assay that can simultaneously detect 4 major vibrio spp., vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae, in the presence of an internal amplification control (iac) was developed. species-specific pcr primers were designed based on the gyrb gene for v. alginolyticus, the collagenase gene for v. parahaemolyticus, the vvha gene for v. vulnificus, and the ompw gene for v. cholerae. additionally, an iac primer pair wa ... | 2014 | 24731836 |
| mechanistic and statistical models of total vibrio abundance in the neuse river estuary. | bacteria in the genus vibrio are ubiquitous to estuarine waters worldwide and are often the dominant genus recovered from these environments. this genus contains several potentially pathogenic species, including vibrio vulnificus, vibrio cholerae, vibrio parahaemolyticus, and vibrio alginolyticus. these bacteria have short generation times, as low as 20-30 min, and can thus respond rapidly to changing environmental conditions. a five-parameter mechanistic model was generated based on environment ... | 2013 | 23948561 |
| temporal and spatial distribution patterns of potentially pathogenic vibrio spp. at recreational beaches of the german north sea. | the number of reported vibrio-related wound infections associated with recreational bathing in northern europe has increased within the last decades. in order to study the health risk from potentially pathogenic vibrio spp. in the central wadden sea, the seasonal and spatial distribution of vibrio vulnificus, vibrio parahaemolyticus, vibrio alginolyticus and vibrio cholerae were investigated at ten recreational beaches in this area over a 2-year period. v. alginolyticus and v. parahaemolyticus w ... | 2013 | 23563708 |
| real-time pcr optimization to identify environmental vibrio spp. strains. | to identify vibrio vulnificus, vibrio cholerae and vibrio alginolyticus using standardized dna extraction method and real-time pcr assays, among a large number of bacterial strains isolated from marine environment. | 2012 | 22639795 |
| occurrence and virulence properties of vibrio and salinivibrio isolates from tropical lagoons of the southern caribbean sea. | the vibrionaceae are gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. the aim of this research was to investigate the occurrence and virulence properties of vibrio and salinivibrio isolated from lagoons at cuare wildlife refuge and margarita island in the southern caribbean sea. water, plankton and oyster samples were collected during october 2011 and march 2012 and examined by s ... | 2017 | 28324231 |
| role of non-coding regulatory rna in the virulence of human pathogenic vibrios. | in recent decades, the identification of small non-coding rnas in bacteria has revealed an important regulatory mechanism of gene expression involved in the response to environmental signals and to the control of virulence. in the family vibrionaceae, which includes several human and animal pathogens, small non-coding rnas (srnas) are closely related to important processes including metabolism, quorum sensing, virulence, and fitness. studies conducted in silico and experiments using microarrays ... | 2016 | 28123382 |
| cross-protection against vibrio cholerae infection by monoclonal antibodies against vibrio vulnificus rtxa1/martxvv. | gram-negative vibrio species secrete multifunctional autoprocessing repeats-in-toxin (martx) toxins associated with bacterial pathogenesis. here, the cross-reactivity and cross-protectivity of mabs against v. vulnificus rtxa1/martxvv was evaluated. passive administration of any of these mabs (21ra, 24ra, 46ra, 47ra and 50ra) provided strong protection against lethal v. cholerae infection. interestingly, 24ra and 46ra, which map to the cysteine protease domain of v. cholerae martxvc , inhibited c ... | 2016 | 27921342 |
| biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries. | the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ... | 2017 | 27871750 |
| non-cholera vibrios: the microbial barometer of climate change. | there is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. one particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. these gram-negative bacteria, including the species vibrio vulnificus, vibrio parahaemolyticus and vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambi ... | 2017 | 27843109 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus in the clam ruditapes philippinarum (adams & reeve, 1850) from emilia romagna and sardinia, italy. | marine vibrios, vibrio parahaemolyticus, v. vulnificus and v. cholerae are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. the aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the manila clam r. philippinarum from emilia romagna (er) and sardinia (sr) regions, italy. isolation was performed on chromagar(tm) vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) agar and m- ... | 2016 | 27800436 |
| quantification of vibrio species in oysters from the gulf of mexico with two procedures based on mpn and pcr. | oysters can accumulate potentially pathogenic water bacteria. the objective of this study was to compare two procedures to quantify vibrio species present in oysters to determine the most sensitive method. we analyzed oyster samples from the gulf of mexico, commercialized in mexico city. the samples were inoculated in tubes with alkaline peptone water (apw), based on three tubes and four dilutions (10(-1) to 10(-4)). from these tubes, the first quantification of vibrio species was performed (mos ... | 2016 | 27709461 |
| prevalence and distribution of vibrio spp. in wild aquatic birds of the southern caribbean sea, venezuela, 2011-12. | vibrio spp. are associated with waterbirds mainly in temperate latitudes. we evaluated the prevalence and distribution of vibrio spp. from fecal samples of resident and migratory aquatic birds collected during october 2011 and march 2012 at two coastal sites in the tropical southern caribbean sea. we amplified dna by pcr in 40% of samples, resulting in 47% and 36% estimated prevalence for resident and migratory birds in cuare wildlife refuge, and 33% and 44% in margarita island, respectively. we ... | 2016 | 27224211 |
| spatiotemporal dynamics of vibrio spp. within the sydney harbour estuary. | vibrio are a genus of marine bacteria that have substantial environmental and human health importance, and there is evidence that their impact may be increasing as a consequence of changing environmental conditions. we investigated the abundance and composition of the vibrio community within the sydney harbour estuary, one of the most densely populated coastal areas in australia, and a region currently experiencing rapidly changing environmental conditions. using quantitative pcr (qpcr) and vibr ... | 2016 | 27148171 |
| isolation and molecular identification of vibrio spp. by sequencing of 16s rdna from seafood, meat and meat products in libya. | the genus vibrio includes several food-borne pathogens that cause a spectrum of clinical conditions including septicemia, cholera and milder forms of gastroenteritis. several vibrio spp. are commonly associated with food-borne transmission including vibrio cholerae, vibrio parahemolyticus, and vibrio vulnificus. microbiological analysis for enumeration and isolation of vibrio spp. were carried out for a total of 93 samples of seafood, meat and meat products from different geographic localities i ... | 2016 | 27004169 |
| detection and quantification of vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus in coastal waters of guinea-bissau (west africa). | v. cholerae, v. parahaemolyticus, and v. vulnificus are recognized human pathogens. although several studies are available worldwide, both on environmental and clinical contexts, little is known about the ecology of these vibrios in african coastal waters. in this study, their co-occurrence and relationships to key environmental constraints in the coastal waters of guinea-bissau were examined using the most probable number-polymerase chain reaction (mpn-pcr) approach. all vibrio species were uni ... | 2016 | 26940502 |
| enterobacteria and vibrio from macrobrachium amazonicum prawn farming in fortaleza, ceará, brazil. | to investigate the isolation of enterobacteria associated with macrobrachium amazonicum (m. amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of vibrio strains. | 2016 | 26851782 |
| occurrence and molecular characterisation of vibrio parahaemolyticus in crustaceans commercialised in venice area, italy. | infections due to the pathogenic human vibrios, vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus, are mainly associated with consumption of raw or partially cooked bivalve molluscs. at present, little is known about the presence of vibrio species in crustaceans and the risk of vibriosis associated with the consumption of these products. the aim of the present study was to evaluate the prevalence and concentration of the main pathogenic vibrio spp. in samples of crustaceans (n=143) ... | 2016 | 26773255 |
| survey on antimicrobial resistance patterns in vibrio vulnificus and vibrio cholerae non-o1/non-o139 in germany reveals carbapenemase-producing vibrio cholerae in coastal waters. | an increase in the occurrence of potentially pathogenic vibrio species is expected for waters in northern europe as a consequence of global warming. in this context, a higher incidence of vibrio infections is predicted for the future and forecasts suggest that people visiting and living at the baltic sea are at particular risk. this study aimed to investigate antimicrobial resistance patterns among vibrio vulnificus and vibrio cholerae non-o1/non-o139 isolates that could pose a public health ris ... | 2015 | 26579088 |
| rapid proliferation of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae during freshwater flash floods in french mediterranean coastal lagoons. | vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae of the non-o1/non-o139 serotype are present in coastal lagoons of southern france. in these mediterranean regions, the rivers have long low-flow periods followed by short-duration or flash floods during and after heavy intense rainstorms, particularly at the end of the summer and in autumn. these floods bring large volumes of freshwater into the lagoons, reducing their salinity. water temperatures recorded during sampling (15 to 24° ... | 2015 | 26319881 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae, and vibrio vulnificus in the aquacultural environments of taiwan. | the occurrence of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae in a total of 72 samples from six aquaculture ponds for groupers, milk fish, and tilapia in southern taiwan was examined by the membrane filtration and colony hybridization method. the halophilic v. parahaemolyticus was only recovered in seawater ponds, with a high isolation frequency of 86.1% and a mean density of 2.6 log cfu/g. v. cholerae was found in both the seawater and freshwater ponds but preferentially in ... | 2015 | 25951392 |
| differential specificity of selective culture media for enumeration of pathogenic vibrios: advantages and limitations of multi-plating methods. | plating environmental samples on vibrio-selective chromogenic media is a commonly used technique that allows one to quickly estimate concentrations of putative vibrio pathogens or to isolate them for further study. although this approach is convenient, its usefulness depends directly on how well the procedure selects against false positives. we tested whether a chromogenic medium, chromagar vibrio (cav), used alone (single-plating) or in combination (double-plating) with a traditional medium thi ... | 2015 | 25602161 |
| abundance of vibrio cholerae, v. vulnificus, and v. parahaemolyticus in oysters (crassostrea virginica) and clams (mercenaria mercenaria) from long island sound. | vibriosis is a leading cause of seafood-associated morbidity and mortality in the united states. typically associated with consumption of raw or undercooked oysters, vibriosis associated with clam consumption is increasingly being reported. however, little is known about the prevalence of vibrio spp. in clams. the objective of this study was to compare the levels of vibrio cholerae, vibrio vulnificus, and vibrio parahaemolyticus in oysters and clams harvested concurrently from long island sound ... | 2014 | 25281373 |
| identification of capsule, biofilm, lateral flagellum, and type iv pili in vibrio mimicus strains. | vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. it is distributed worldwide. factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. in pathogenic vibrio species such as v. cholerae, vibrio. parahaemolyticus and vibrio vulnificu ... | 2014 | 25246027 |
| pathogenic vibrios in environmental, seafood and clinical sources in germany. | bacteria of the family vibrionaceae naturally occur in marine and estuarine environments. only few species of vibrionaceae are associated with human cases of gastroenteritis, ear and wound infections, caused by ingestion of seafood or contact with vibrio containing water. increasing consumption of seafood (fish, fishery products and shellfish) poses a possible source of vibrio infections in germany. additionally, there is a growing concern that abundances of pathogenic vibrios may increase in ge ... | 2014 | 25129553 |
| occurrence of the three major vibrio species pathogenic for human in seafood products consumed in france using real-time pcr. | vibrio spp. have emerged as a serious threat to human health worldwide. vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus are of particular concern as they have been linked to gastrointestinal infections and septicemia associated with the consumption of raw or undercooked seafood. we developed hydrolysis probe-based real-time pcr systems with an internal amplification control for the detection of these species. we applied these systems to a total of 167 fresh or frozen crustacean, f ... | 2014 | 25128747 |
| sequence and expression divergence of an ancient duplication of the chaperonin groesel operon in vibrio species. | heat-shock proteins are molecular chaperones essential for protein folding, degradation and trafficking. the human pathogen vibrio vulnificus encodes a copy of the groesel operon in both chromosomes and these genes share <80 % similarity with each other. comparative genomic analysis was used to determine whether this duplication is prevalent among vibrionaceae specifically or gammaproteobacteria in general. among the vibrionaceae complete genome sequences in the database (31 species), seven vibr ... | 2014 | 24913685 |
| glycan specificity of the vibrio vulnificus hemolysin lectin outlines evolutionary history of membrane targeting by a toxin family. | pore-forming toxins (pfts) are a class of pathogen-secreted molecules that oligomerize to form transmembrane channels in cellular membranes. determining the mechanism for how pfts bind membranes is important in understanding their role in disease and for developing possible ways to block their action. vibrio vulnificus, an aquatic pathogen responsible for severe food poisoning and septicemia in humans, secretes a pft called v. vulnificus hemolysin (vvh), which contains a single c-terminal target ... | 2014 | 24862282 |
| vibrio vulnificus phage pv94 is closely related to temperate phages of v. cholerae and other vibrio species. | vibrio vulnificus is an important pathogen which can cause serious infections in humans. yet, there is limited knowledge on its virulence factors and the question whether temperate phages might be involved in pathogenicity, as is the case with v. cholerae. thus far, only two phages (ssp002 and vvaw1) infecting v. vulnificus have been genetically characterized. these phages were isolated from the environment and are not related to vibrio cholerae phages. the lack of information on temperate v. vu ... | 2014 | 24732980 |
| high-salt preadaptation of vibrio parahaemolyticus enhances survival in response to lethal environmental stresses. | adaptation to changing environmental conditions is an important strategy for survival of foodborne bacterial pathogens. vibrio parahaemolyticus is a gram-negative seafoodborne enteric pathogen found in the marine environment both free living and associated with oysters. this pathogen is a moderate halophile, with optimal growth at 3% nacl. among the several stresses imposed upon enteric bacteria, acid stress is perhaps one of the most important. v. parahaemolyticus has a lysine decarboxylase sys ... | 2014 | 24490918 |
| relationship of aquatic environmental factors with the abundance of vibrio cholerae, vibrio parahaemolyticus, vibrio mimicus and vibrio vulnificus in the coastal area of guaymas, sonora, mexico. | members of the genus vibrio are common in aquatic environments. among them are v. cholerae, v. vulnificus, v. parahaemolyticus and v. mimicus. several studies have shown that environmental factors, such as temperature, salinity, and dissolved oxygen, are involved in their epidemiology. therefore, the main objective of this study is to determine if there is a correlation between the presence/amount of v. cholerae, v, vulnificus, v. parahaemolyticus and v. mimicus and the environmental conditions ... | 2013 | 24334844 |
| multiplex real-time polymerase chain reaction assays for simultaneous detection of vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus. | a multiplex real-time polymerase chain reaction (rt-pcr) method was developed for the identification of three vibrio species: vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus. | 2013 | 24159544 |
| detection of vibrio parahaemolyticus, vibrio vulnificus and vibrio cholerae with respect to seasonal fluctuations in temperature and plankton abundance. | over a 1-year period, bi-monthly estuarine surface water and plankton samples (63-200 and > 200 μm fractions) were assayed by polymerase chain reaction for the prevalence of total vibrio parahaemolyticus, v. vulnificus and v. cholerae and select genes associated with clinical strains found in each species. neither temperature nor plankton abundance was a significant correlate of total v. parahaemolyticus; however, the prevalence of genes commonly associated with clinical strains (trh, tdh, orf8) ... | 2014 | 24024909 |
| taxonomic and functional metagenomic profiling of gastrointestinal tract microbiome of the farmed adult turbot (scophthalmus maximus). | metagenomics combined with 16s rrna gene sequence analyses was applied to unveil the taxonomic composition and functional diversity of the farmed adult turbot gastrointestinal (gi) microbiome. proteobacteria and firmicutes which existed in both gi content and mucus were dominated in the turbot gi microbiome. 16s rrna gene sequence analyses also indicated that the turbot gi tract may harbor some bacteria which originated from associated seawater. functional analyses indicated that the clustering- ... | 2013 | 23802730 |
| quantification of vibrio parahaemolyticus, vibrio vulnificus and vibrio cholerae in french mediterranean coastal lagoons. | vibrio parahaemolyticus, vibrio vulnificus and vibrio cholerae are human pathogens. little is known about these vibrio spp. in the coastal lagoons of france. the purpose of this study was to investigate their incidence in water, shellfish and sediment of three french mediterranean coastal lagoons using the most probable number-polymerase chain reaction (mpn-pcr). in summer, the total number of v. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from ... | 2013 | 23770313 |
| abundance and distribution of vibrio cholerae, v. parahaemolyticus, and v. vulnificus following a major freshwater intrusion into the mississippi sound. | in response to a major influx of freshwater to the mississippi sound following the opening of the bonnet carre spillway, water samples were collected from three sites along the mississippi shoreline to assess the impact of altered salinity on three pathogenic vibrio species. salinity readings across the affected area during the 2011 sample period ranged from 1.4 to 12.9 ppt (mean = 7.0) and for the 2012 sample period from 14.1 to 23.6 ppt (mean = 19.8). analyses of the data collected in 2011 sho ... | 2013 | 23494573 |
| genome sequence of the human-pathogenic bacterium vibrio vulnificus type strain atcc 27562. | vibrio vulnificus, which is, like vibrio cholerae and vibrio parahaemolyticus, a pathogen of humankind, is a gram-negative, curved, motile, and rod-shaped bacterium [corrected] here, we present the draft genome sequence of the type strain, atcc 27562, which was the first isolated vibrio vulnificus strain. | 2012 | 23209214 |
| development of a groel gene-based species-specific multiplex polymerase chain reaction assay for simultaneous detection of vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. | to develop an effective multiplex polymerase chain reaction (pcr) for the simultaneous detection of three important vibrio species, vibrio cholerae (vc), v. parahaemolyticus (vp) and v. vulnificus (vv) using the groel gene, a potential phylogenetic marker. | 2013 | 23121500 |